BR0112566A - Polinucleotìdeo, polinucleotìdeo isolado, construção quimérica, célula hospedeira isolada, célula hospedeira, método de seleção de um polinucleotìdeo isolado, método de obtenção de um fragmento de ácido nucléico e método para a produção de ácidos graxos - (delta)¹²-epóxi em uma célula hospedeira - Google Patents
Polinucleotìdeo, polinucleotìdeo isolado, construção quimérica, célula hospedeira isolada, célula hospedeira, método de seleção de um polinucleotìdeo isolado, método de obtenção de um fragmento de ácido nucléico e método para a produção de ácidos graxos - (delta)¹²-epóxi em uma célula hospedeiraInfo
- Publication number
- BR0112566A BR0112566A BR0112566-4A BR0112566A BR0112566A BR 0112566 A BR0112566 A BR 0112566A BR 0112566 A BR0112566 A BR 0112566A BR 0112566 A BR0112566 A BR 0112566A
- Authority
- BR
- Brazil
- Prior art keywords
- isolated
- polynucleotide
- host cell
- cell
- fatty acids
- Prior art date
Links
- 239000004593 Epoxy Substances 0.000 title abstract 4
- 235000014113 dietary fatty acids Nutrition 0.000 title abstract 4
- 229930195729 fatty acid Natural products 0.000 title abstract 4
- 239000000194 fatty acid Substances 0.000 title abstract 4
- 150000004665 fatty acids Chemical class 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 4
- 239000002157 polynucleotide Substances 0.000 title abstract 4
- 102000040430 polynucleotide Human genes 0.000 title abstract 4
- 108091033319 polynucleotide Proteins 0.000 title abstract 4
- 238000004519 manufacturing process Methods 0.000 title abstract 3
- 150000007523 nucleic acids Chemical group 0.000 title abstract 2
- 102000002004 Cytochrome P-450 Enzyme System Human genes 0.000 abstract 3
- 108010015742 Cytochrome P-450 Enzyme System Proteins 0.000 abstract 3
- 230000015572 biosynthetic process Effects 0.000 abstract 3
- 238000003786 synthesis reaction Methods 0.000 abstract 3
- 238000010276 construction Methods 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 235000013311 vegetables Nutrition 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
- C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/02—Oxygen as only ring hetero atoms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
Abstract
"POLINUCLEOTìDEO, POLINUCLEOTìDEO ISOLADO, CONSTRUçãO QUIMéRICA, CéLULA HOSPEDEIRA ISOLADA, CéLULA HOSPEDEIRA, MéTODO DE SELEçãO DE UM POLINUCLEOTìDEO ISOLADO, MéTODO DE OBTENçãO DE UM FRAGMENTO DE áCIDO NUCLéICO E MéTODO PARA A PRODUçãO DE áCIDOS GRAXOS A <30>^ 12^-EPóXI EM UMA CéLULA HOSPEDEIRA". é descrito um fragmento de ácido nucléico isolado codificador de uma enzima citocromo P450 associada à síntese de ácidos graxos <30>^ 12^-epóxi vegetais. A presente invenção também se refere à produção de uma construção quimérica que codifique as enzimas citocromo P450, no todo ou em parte, associadas à síntese de ácidos graxos <30>^ 12^-epóxi vegetais, em orientação com sentido ou sem sentido, em que a expressão da construção quimérica resulta na produção de níveis alterados das enzimas citocromo P450 associadas à síntese de ácidos graxos <30>^ 12^-epóxi vegetal em células hospedeiras transformadas.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US21983300P | 2000-07-21 | 2000-07-21 | |
PCT/US2001/022790 WO2002008269A2 (en) | 2000-07-21 | 2001-07-19 | A cytochrome p450 enzyme associated with the synthesis of δ12-epoxy groups in fatty acids of plants |
Publications (1)
Publication Number | Publication Date |
---|---|
BR0112566A true BR0112566A (pt) | 2004-02-10 |
Family
ID=22820966
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR0112566-4A BR0112566A (pt) | 2000-07-21 | 2001-07-19 | Polinucleotìdeo, polinucleotìdeo isolado, construção quimérica, célula hospedeira isolada, célula hospedeira, método de seleção de um polinucleotìdeo isolado, método de obtenção de um fragmento de ácido nucléico e método para a produção de ácidos graxos - (delta)¹²-epóxi em uma célula hospedeira |
Country Status (16)
Country | Link |
---|---|
US (2) | US20030066103A1 (pt) |
EP (1) | EP1309698B1 (pt) |
JP (1) | JP2004513623A (pt) |
AT (1) | ATE360075T1 (pt) |
AU (1) | AU2001277924A1 (pt) |
BR (1) | BR0112566A (pt) |
CA (1) | CA2410663C (pt) |
DE (1) | DE60127967T2 (pt) |
DK (1) | DK1309698T3 (pt) |
ES (1) | ES2284666T3 (pt) |
HU (1) | HUP0400496A3 (pt) |
MX (1) | MXPA03000507A (pt) |
NO (1) | NO20030229L (pt) |
PT (1) | PT1309698E (pt) |
SI (1) | SI1309698T1 (pt) |
WO (1) | WO2002008269A2 (pt) |
Families Citing this family (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7364901B2 (en) | 2002-07-15 | 2008-04-29 | University Of Kentucky Research Foundation | Recombinant Stokesia epoxygenase gene |
US20040172682A1 (en) | 2003-02-12 | 2004-09-02 | Kinney Anthony J. | Production of very long chain polyunsaturated fatty acids in oilseed plants |
US8084074B2 (en) | 2003-02-12 | 2011-12-27 | E. I. Du Pont De Nemours And Company | Production of very long chain polyunsaturated fatty acids in oil seed plants |
EP1685239B1 (en) | 2003-11-12 | 2014-05-21 | E.I. Du Pont De Nemours And Company | Delta-15 desaturases suitable for altering levels of polyunsaturated fatty acids in oilseed plants and oleaginous yeast |
US7256033B2 (en) | 2004-06-25 | 2007-08-14 | E. I. Du Pont De Nemours And Company | Delta-8 desaturase and its use in making polyunsaturated fatty acids |
US20060156430A1 (en) * | 2005-01-13 | 2006-07-13 | Mcgonigle Brian | Novel cytochrome P450 monooxygenase |
US20070199096A1 (en) | 2005-11-14 | 2007-08-23 | E.I. Du Pont De Nemours And Company | Compositions and Methods for Altering Alpha- and Beta-Tocotrienol Content |
CA2625855C (en) | 2005-11-23 | 2016-04-19 | E. I. Du Pont De Nemours And Company | Delta-9 elongases and their use in making polyunsaturated fatty acids |
US7943823B2 (en) | 2006-04-28 | 2011-05-17 | E.I. Du Pont De Nemours And Company | Delta-8 desaturase and its use in making polyunsaturated fatty acids |
US7695950B2 (en) | 2006-05-17 | 2010-04-13 | E. I. Du Pont De Nemours And Company | Δ5 desaturase and its use in making polyunsaturated fatty acids |
BRPI0716306A2 (pt) | 2006-10-23 | 2015-06-16 | Du Pont | "polinucleotídeo isolado, constructo de dna recombinante célula, yarrowia sp transformada, método para transformar uma célula, método para produzir uma planta transformada, método para produzir leveduras, sementes trangênicas, método para produzir ácidos graxos poli-insaturados de cadeia longa em ima célula, óleo ou subprodutos, método para produzir pelo menos um ácido graxo poli-insaturada em uma célula vegetal oleaginosa, plantas oleaginosas, sementes trangênicas, alimentos ou alimentações e plantas da progênie" |
BRPI0806354A2 (pt) | 2007-02-12 | 2011-09-06 | Du Pont | plantas oleaginosas transgências, sementes, óleos, produtos alimentìcios ou análogos a alimento, produtos alimentìcios medicinais ou análogos alimentìcios medicinais, produtos farmacêuticos, bebidas fórmulas para bebês, suplementos nutricionais, rações para animais domésticos, alimentos para aquacultura, rações animais, produtos de sementes inteiras, produtos de óleos misturados, produtos, subprodutos e subprodutos parcialmente processados |
US7790156B2 (en) | 2007-04-10 | 2010-09-07 | E. I. Du Pont De Nemours And Company | Δ-8 desaturases and their use in making polyunsaturated fatty acids |
US8119860B2 (en) | 2007-04-16 | 2012-02-21 | E. I. Du Pont De Nemours And Company | Delta-9 elongases and their use in making polyunsaturated fatty acids |
US7943365B2 (en) | 2007-05-03 | 2011-05-17 | E.I. Du Pont De Nemours And Company | Δ-5 desaturases and their use in making polyunsaturated fatty acids |
CA2685309C (en) | 2007-05-24 | 2019-06-25 | E. I. Du Pont De Nemours And Company | Dgat genes from yarrowia lipolytica for increased seed storage lipid production and altered fatty acid profiles in soybean |
EP2620500A3 (en) | 2008-05-23 | 2014-01-08 | E. I. du Pont de Nemours and Company | DGAT genes from oleaginous organisms for increased seed storage lipid production and altered fatty acid profiles in oilseed plants |
WO2010114989A1 (en) | 2009-04-01 | 2010-10-07 | E. I. Du Pont De Nemours And Company | Use of a seed specific promoter to drive odp1 expression in cruciferous oilseed plants to increase oil content while maintaining normal germination |
AR078829A1 (es) | 2009-10-30 | 2011-12-07 | Du Pont | Plantas y semillas con niveles alterados de compuesto de almacenamiento, construcciones relacionadas y metodos relacionados con genes que codifican proteinas similares a las aldolasas bacterianas de la clase ii del acido 2,4- dihidroxi-hept-2-eno-1,7-dioico |
US8637733B2 (en) | 2009-12-24 | 2014-01-28 | E.I. Du Pont De Nemours And Company | Plant membrane bound O-acyl transferase (MBOAT) family protein sequences and their uses for altering fatty acid compositions |
BR112012022045A8 (pt) | 2010-03-03 | 2018-06-19 | Du Pont | ''planta transgênica,semente transgênica,método para produzir uma planta transgênica,método para produzir sementes transgênicas,produto e/ou subproduto,polinucleotídeo isolado e planta ou semente''. |
AU2011271497A1 (en) | 2010-07-01 | 2013-01-10 | E. I. Du Pont De Nemours And Company | Plant seeds with altered storage compound levels, related constructs and methods involving genes encoding PAE and PAE-like polypeptides |
US10036030B2 (en) | 2011-12-22 | 2018-07-31 | E I Du Pont De Nemours And Company | Use of the soybean sucrose synthase promoter to increase plant seed lipid content |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5190931A (en) * | 1983-10-20 | 1993-03-02 | The Research Foundation Of State University Of New York | Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA |
US4945050A (en) * | 1984-11-13 | 1990-07-31 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues and apparatus therefor |
US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US5283323A (en) * | 1985-08-07 | 1994-02-01 | The United States Of America As Represented By The Department Of Health And Human Services | Method of producing immune response |
US4800159A (en) * | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
US5107065A (en) * | 1986-03-28 | 1992-04-21 | Calgene, Inc. | Anti-sense regulation of gene expression in plant cells |
US5231020A (en) * | 1989-03-30 | 1993-07-27 | Dna Plant Technology Corporation | Genetic engineering of novel plant phenotypes |
DK0975765T3 (da) * | 1997-04-15 | 2007-02-19 | Commw Scient Ind Res Org | Plantefedtsyre-epoxygenase-gener og anvendelse deraf |
US5846784A (en) * | 1997-06-11 | 1998-12-08 | E. I. Du Pont De Nemours And Company | Fatty acid modifying enzymes from developing seeds of Vernonia galamenensis |
US6083731A (en) * | 1997-06-24 | 2000-07-04 | Washington State University Research Foundation | Recombinant materials and methods for the production of limonene hydroxylases |
-
2001
- 2001-07-19 DK DK01955872T patent/DK1309698T3/da active
- 2001-07-19 HU HU0400496A patent/HUP0400496A3/hu unknown
- 2001-07-19 WO PCT/US2001/022790 patent/WO2002008269A2/en active IP Right Grant
- 2001-07-19 DE DE60127967T patent/DE60127967T2/de not_active Expired - Lifetime
- 2001-07-19 SI SI200130724T patent/SI1309698T1/sl unknown
- 2001-07-19 CA CA2410663A patent/CA2410663C/en not_active Expired - Fee Related
- 2001-07-19 MX MXPA03000507A patent/MXPA03000507A/es active IP Right Grant
- 2001-07-19 JP JP2002514173A patent/JP2004513623A/ja active Pending
- 2001-07-19 EP EP01955872A patent/EP1309698B1/en not_active Expired - Lifetime
- 2001-07-19 PT PT01955872T patent/PT1309698E/pt unknown
- 2001-07-19 AT AT01955872T patent/ATE360075T1/de not_active IP Right Cessation
- 2001-07-19 ES ES01955872T patent/ES2284666T3/es not_active Expired - Lifetime
- 2001-07-19 AU AU2001277924A patent/AU2001277924A1/en not_active Abandoned
- 2001-07-19 BR BR0112566-4A patent/BR0112566A/pt not_active IP Right Cessation
- 2001-07-20 US US09/909,566 patent/US20030066103A1/en not_active Abandoned
-
2003
- 2003-01-17 NO NO20030229A patent/NO20030229L/no unknown
-
2004
- 2004-01-15 US US10/759,813 patent/US20040139499A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
WO2002008269A3 (en) | 2003-02-27 |
NO20030229D0 (no) | 2003-01-17 |
DK1309698T3 (da) | 2007-06-04 |
CA2410663A1 (en) | 2002-01-31 |
AU2001277924A1 (en) | 2002-02-05 |
JP2004513623A (ja) | 2004-05-13 |
US20040139499A1 (en) | 2004-07-15 |
DE60127967T2 (de) | 2008-01-17 |
SI1309698T1 (sl) | 2007-08-31 |
DE60127967D1 (de) | 2007-05-31 |
MXPA03000507A (es) | 2003-10-06 |
PT1309698E (pt) | 2007-06-22 |
WO2002008269A2 (en) | 2002-01-31 |
EP1309698B1 (en) | 2007-04-18 |
NO20030229L (no) | 2003-03-04 |
ES2284666T3 (es) | 2007-11-16 |
HUP0400496A3 (en) | 2005-06-28 |
ATE360075T1 (de) | 2007-05-15 |
EP1309698A2 (en) | 2003-05-14 |
CA2410663C (en) | 2011-12-13 |
HUP0400496A2 (hu) | 2004-06-28 |
US20030066103A1 (en) | 2003-04-03 |
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B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
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