BR0014857A - Preparação e seleção de células doadoras para transplante nuclear - Google Patents

Preparação e seleção de células doadoras para transplante nuclear

Info

Publication number
BR0014857A
BR0014857A BR0014857-1A BR0014857A BR0014857A BR 0014857 A BR0014857 A BR 0014857A BR 0014857 A BR0014857 A BR 0014857A BR 0014857 A BR0014857 A BR 0014857A
Authority
BR
Brazil
Prior art keywords
selection
cells
nuclear
cell preparation
donor cell
Prior art date
Application number
BR0014857-1A
Other languages
English (en)
Inventor
James M Robl
Kasinathan Poothappillai
Jason Knott
Joseph D Jerry
Original Assignee
Univ Ofd Massachusetts Public
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Ofd Massachusetts Public filed Critical Univ Ofd Massachusetts Public
Publication of BR0014857A publication Critical patent/BR0014857A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/10—Conditioning of cells for in vitro fecondation or nuclear transfer

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)

Abstract

"PREPARAçãO E SELEçãO DE CéLULAS DOADORAS PARA TRANSPLANTE NUCLEAR". A presente invenção refere-se a um método de sincronização de uma população de células somáticas em G~ 1~, com a finalidade de preparar as células para transferência nuclear ou transplante nuclear, mediante uso de agitação mecânica e seleção de células mitóticas parelhas. Esse método pode compreender ainda o resfriamento das células ou outros meios de sincronização das células na fase G~ 1~, por períodos de tempo mais longos. A invenção também refere-se ao uso de uma população sincronizada de células somáticas obtidas por esses métodos que se dividem rapidamente, como uma fonte de núcleos doadores ou de cromatina para uso na transferência nuclear ou no transplante nuclear.
BR0014857-1A 1999-10-14 2000-10-13 Preparação e seleção de células doadoras para transplante nuclear BR0014857A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15936899P 1999-10-14 1999-10-14
PCT/US2000/028287 WO2001026454A1 (en) 1999-10-14 2000-10-13 Preparation and selection of donor cells for nuclear transplantation

Publications (1)

Publication Number Publication Date
BR0014857A true BR0014857A (pt) 2003-07-08

Family

ID=22572315

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0014857-1A BR0014857A (pt) 1999-10-14 2000-10-13 Preparação e seleção de células doadoras para transplante nuclear

Country Status (11)

Country Link
US (1) US20050273870A1 (pt)
EP (1) EP1225800A1 (pt)
JP (1) JP2003514515A (pt)
CN (1) CN1209457C (pt)
AU (1) AU781128B2 (pt)
BR (1) BR0014857A (pt)
CA (1) CA2387488A1 (pt)
IL (1) IL149071A0 (pt)
MX (1) MXPA02003732A (pt)
NZ (1) NZ518244A (pt)
WO (1) WO2001026454A1 (pt)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020142397A1 (en) 2000-12-22 2002-10-03 Philippe Collas Methods for altering cell fate
CN100357316C (zh) * 2004-06-23 2007-12-26 中国科学技术大学 人类细胞中体的蛋白质复合物的分离方法
EP1984487B1 (en) 2005-08-03 2022-10-12 Astellas Institute for Regenerative Medicine Improved methods of reprogramming animal somatic cells
EP3190178A1 (en) 2007-02-23 2017-07-12 Astellas Institute for Regenerative Medicine Highly efficient methods for reprogramming differentiated cells and for generating animals and embryonic stem cells from reprogrammed cells
WO2009005844A1 (en) * 2007-07-02 2009-01-08 Gregory Aharonian Methods for female mammalian spermatogenesis and male mammalian oogenesis using synthetic nanobiology
US20090170203A1 (en) * 2008-01-01 2009-07-02 Aharonian Gregory P Methods for female mammalian spermatogenesis and male mammalian oogenesis using synthetic nanobiology
JP2010075099A (ja) * 2008-09-26 2010-04-08 Japan Science & Technology Agency 哺乳動物核移植胚のスクリーニング方法、非ヒト哺乳動物核移植胚、クローン非ヒト哺乳動物、及びスクリーニングキット
CZ302682B6 (cs) * 2009-04-16 2011-08-31 Univerzita Palackého Zpusob produkce synchronizovaných adherentne rostoucích bunecných linií a prístroj pro provádení tohoto zpusobu
WO2013010045A1 (en) 2011-07-12 2013-01-17 Biotime Inc. Novel methods and formulations for orthopedic cell therapy
WO2016036754A1 (en) * 2014-09-02 2016-03-10 The Regents Of The University Of California Methods and compositions for rna-directed target dna modification
CN115433684A (zh) * 2022-10-20 2022-12-06 云南师范大学 一种盘基网柄菌细胞同步化方法

Also Published As

Publication number Publication date
CN1399513A (zh) 2003-02-26
JP2003514515A (ja) 2003-04-22
WO2001026454A1 (en) 2001-04-19
WO2001026454A9 (en) 2002-08-01
AU1200101A (en) 2001-04-23
MXPA02003732A (es) 2004-09-10
CA2387488A1 (en) 2001-04-19
EP1225800A1 (en) 2002-07-31
US20050273870A1 (en) 2005-12-08
NZ518244A (en) 2003-10-31
AU781128B2 (en) 2005-05-05
CN1209457C (zh) 2005-07-06
IL149071A0 (en) 2009-02-11

Similar Documents

Publication Publication Date Title
BR0014857A (pt) Preparação e seleção de células doadoras para transplante nuclear
BR0113216A (pt) Criopreservação de esperma
BR0209083A (pt) Métodos e reagentes para transplante celular
BR8707673A (pt) Processo para reproduzir medula ossea
MXPA02006094A (es) Metodos para producir embriones clonadod y adultos a partir de celulas cultivadas.
EP1254210A4 (en) METHOD FOR CLONING ANIMALS WITH TARGETED GENETIC CHANGES BY TRANSFER OF MALE OR FEMALE SOMATIC CELL CORES FROM LONG-TERM CULTURE INCLUDING ARTIFICIALLY INDUCED GENETIC CHANGES IN CORE RECEIVERS
BR0008536A (pt) Usos de um meio de cultura, e de pelo menos um mas, análogo de mas ou um aditivo ou aditivos, e, processo para fertilização in vitro
DK1140149T3 (da) Anvendelse af humant væksthormon til forögelse af antallet af cirkulerende CD34+-celler, tilsigtet til genetablering af de hematopoietiske system og immunsystemet fölgende myeloablative eller antibalstiske terapier ved transplantation......
Collas Formation of the sea urchin male pronucleus in cell‐free extracts
AU3927599A (en) Source of nuclei for nuclear transfer
Pan The Cultivation of Comprehensive Abilities of Clinical Postgraduate
Gupta et al. In vitro culture of skin fibroblast cells for potential cloning by nuclear transfer
Zhang Plasticity of bone marrow stem cells
Jun et al. From Adult Bone Marrow Cells to Other Cell Lineages: Transdifferentiation or Cells Fusion
Zhang Set up a course of Critical Thinking Improve the ability of clinical thought
LI et al. Mechanisms of cholic acid for reducing damage to human live cell lines L-O2 induced by amanita toxic peptides
Kao et al. Exploration into the Cultivation of Teaching Ability of Clinical Graduate
Abdelaziz et al. Risk perception for hepatitis B and C among Tanta university students
LI et al. The Experience of Bilingual Teaching in Surgery
Wang et al. The Effects of Carbamates and Soman on the Metabolism of Nicotinic Acetylcholine Receptors in Cultured Skeletal Muscle Cells
XU Antiapoptotic effects of rat hypertrophic cardiomyocytes induced with hypoxia/reoxygenation by dichloroacetate
Liu et al. Taking the opportunity of clinical skill center construction to promote the reforms on the teaching of clinical practice
Na et al. Effects of dietary forage-to-concentrate ratio on nutrient digestibility and enteric methane production in growing goats () and Sika deer ()
PT1185625E (pt) Metodo para a preparacao de material celular neuronal
Bondioli In vitro fertilization of ovine and bovine oocytes.

Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 9A , 10A E 11A ANUIDADES.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2138 DE 27/12/2011 E CONSIDERANDO A AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS. INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE.