BE902457A - METHOD FOR EXAMINING CELL SCREENING. - Google Patents

METHOD FOR EXAMINING CELL SCREENING.

Info

Publication number
BE902457A
BE902457A BE0/215039A BE215039A BE902457A BE 902457 A BE902457 A BE 902457A BE 0/215039 A BE0/215039 A BE 0/215039A BE 215039 A BE215039 A BE 215039A BE 902457 A BE902457 A BE 902457A
Authority
BE
Belgium
Prior art keywords
cells
capsules
cell
cell screening
substance
Prior art date
Application number
BE0/215039A
Other languages
French (fr)
Inventor
A P Jarvis Jr
G A Koch
P G Abrams
Original Assignee
Damon Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Damon Biotech Inc filed Critical Damon Biotech Inc
Publication of BE902457A publication Critical patent/BE902457A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C12N5/163—Animal cells one of the fusion partners being a B or a T lymphocyte
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Procédé d'examen de dépistage de cellules par lequel on encapsule des cellules (génétiquement modifiées et/ou hybridomes) au sein de membranes perméables à une substance intéressante sécrétée par la cellule à dépister, mais sensiblement imperméables aux cellules. Dans un appareil à plusieurs compartiments, dont chacun contient un milieu extracapsulaire et des capsules contenant les cellules, on cultive les cellules dans les capsules. La substance intéressante, sécrétée dans la capsule, traverse la membrane et passe dans le milieu extracapsulaire que l'on examine. On répète le processus pour les capsules des compartiments positifs, jusqu'à identification d'une cellule secrétant la substance intéressante.Cell screening examination method by which cells (genetically modified and / or hybridomas) are encapsulated within membranes permeable to an interesting substance secreted by the cell to be screened, but substantially impermeable to cells. In a multi-compartment apparatus, each of which contains an extracapsular medium and capsules containing the cells, the cells are cultured in the capsules. The interesting substance, secreted in the capsule, crosses the membrane and passes into the extracapsular medium which is examined. The process is repeated for the capsules of the positive compartments, until a cell secreting the substance of interest is identified.

BE0/215039A 1984-05-24 1985-05-20 METHOD FOR EXAMINING CELL SCREENING. BE902457A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US61323584A 1984-05-24 1984-05-24

Publications (1)

Publication Number Publication Date
BE902457A true BE902457A (en) 1985-09-16

Family

ID=24456442

Family Applications (1)

Application Number Title Priority Date Filing Date
BE0/215039A BE902457A (en) 1984-05-24 1985-05-20 METHOD FOR EXAMINING CELL SCREENING.

Country Status (11)

Country Link
JP (1) JPS60250256A (en)
AU (1) AU3450684A (en)
BE (1) BE902457A (en)
DE (1) DE3509210A1 (en)
DK (1) DK57885A (en)
FR (1) FR2564858A1 (en)
GB (1) GB2159171A (en)
IL (1) IL73344A0 (en)
IT (1) IT1179899B (en)
NL (1) NL8500451A (en)
SE (1) SE8405105L (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4130272A (en) * 1977-09-22 1978-12-19 Emmie George W Flexible picket fence
CA1232849A (en) * 1983-12-01 1988-02-16 The Washington University Circulating antigens of dirofilaria immitis, monoclonal antibodies specific therefor and methods of preparing such antibodies and detecting such antigens
US4686098A (en) * 1984-05-14 1987-08-11 Merck & Co., Inc. Encapsulated mouse cells transformed with avian retrovirus-bovine growth hormone DNA, and a method of administering BGH in vivo
US4680174A (en) * 1984-05-24 1987-07-14 Damon Biotech, Inc. Induction of immune response by immunization with encapsulated antigen-producing cells
IL79052A0 (en) * 1986-06-06 1986-11-30 Univ Ramot Device and process for production of alginate-shell beads containing biologically active material
EP0975969A4 (en) * 1997-03-18 2002-07-31 Chromaxome Corp Methods for screening compounds using encapsulated cells
EP2668284B1 (en) * 2011-01-28 2014-10-15 Amyris, Inc. Gel-encapsulated microcolony screening

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4352883A (en) * 1979-03-28 1982-10-05 Damon Corporation Encapsulation of biological material
US4663286A (en) * 1984-02-13 1987-05-05 Damon Biotech, Inc. Encapsulation of materials

Also Published As

Publication number Publication date
GB2159171A (en) 1985-11-27
SE8405105D0 (en) 1984-10-12
JPS60250256A (en) 1985-12-10
GB8428320D0 (en) 1984-12-19
DE3509210A1 (en) 1985-11-28
SE8405105L (en) 1985-11-25
FR2564858A1 (en) 1985-11-29
DK57885D0 (en) 1985-02-07
DE3509210C2 (en) 1988-03-31
NL8500451A (en) 1985-12-16
IT1179899B (en) 1987-09-16
DK57885A (en) 1985-11-25
IL73344A0 (en) 1985-01-31
AU3450684A (en) 1985-11-28
IT8468287A0 (en) 1984-12-28

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Legal Events

Date Code Title Description
RE Patent lapsed

Owner name: DAMON BIOTECH INC.

Effective date: 19870531