SE8405105D0 - SET FOR SCREENING CELLS - Google Patents

SET FOR SCREENING CELLS

Info

Publication number
SE8405105D0
SE8405105D0 SE8405105A SE8405105A SE8405105D0 SE 8405105 D0 SE8405105 D0 SE 8405105D0 SE 8405105 A SE8405105 A SE 8405105A SE 8405105 A SE8405105 A SE 8405105A SE 8405105 D0 SE8405105 D0 SE 8405105D0
Authority
SE
Sweden
Prior art keywords
substance
cells
cell
capsules
compartment
Prior art date
Application number
SE8405105A
Other languages
Swedish (sv)
Other versions
SE8405105L (en
Inventor
Jr A P Jarvis
G A Koch
P G Abrams
Original Assignee
Damon Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Damon Biotech Inc filed Critical Damon Biotech Inc
Publication of SE8405105D0 publication Critical patent/SE8405105D0/en
Publication of SE8405105L publication Critical patent/SE8405105L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C12N5/163—Animal cells one of the fusion partners being a B or a T lymphocyte
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

An improved process for screening cell cultures, e.g., cultures of genetically modified cells or hybridomas, for cells secreting a specific substance involves encapsulating cells within membranes permeable to the substance of interest but substantially impermeable to the cells. The cell-containing capsules are cultured in an apparatus having plural compartments, e.g. a microtiter tray, each compartment containing an extracapsular medium and capsules containing cells. The encapsulated cell producing the substance of interest synthesizes and secretes the substance into the intracapsular volume of the capsule and the secreted substance passes through the membrane into the extracapsular medium. The extracapsular medium of each compartment is assayed for the substance and the process is repeated for the capsules from all compartments yielding a positive assay. Thus, by separating the capsules in a positive-assaying compartment from one another and placing them anew in separate compartments, the assay can be repeated to locate that compartment containing the cell which secretes the substance of interest, thereby to identify the cell and differentiate it from other cells.
SE8405105A 1984-05-24 1984-10-12 SET FOR SCREENING CELLS SE8405105L (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US61323584A 1984-05-24 1984-05-24

Publications (2)

Publication Number Publication Date
SE8405105D0 true SE8405105D0 (en) 1984-10-12
SE8405105L SE8405105L (en) 1985-11-25

Family

ID=24456442

Family Applications (1)

Application Number Title Priority Date Filing Date
SE8405105A SE8405105L (en) 1984-05-24 1984-10-12 SET FOR SCREENING CELLS

Country Status (11)

Country Link
JP (1) JPS60250256A (en)
AU (1) AU3450684A (en)
BE (1) BE902457A (en)
DE (1) DE3509210A1 (en)
DK (1) DK57885A (en)
FR (1) FR2564858A1 (en)
GB (1) GB2159171A (en)
IL (1) IL73344A0 (en)
IT (1) IT1179899B (en)
NL (1) NL8500451A (en)
SE (1) SE8405105L (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4130272A (en) * 1977-09-22 1978-12-19 Emmie George W Flexible picket fence
CA1232849A (en) * 1983-12-01 1988-02-16 The Washington University Circulating antigens of dirofilaria immitis, monoclonal antibodies specific therefor and methods of preparing such antibodies and detecting such antigens
US4686098A (en) * 1984-05-14 1987-08-11 Merck & Co., Inc. Encapsulated mouse cells transformed with avian retrovirus-bovine growth hormone DNA, and a method of administering BGH in vivo
US4680174A (en) * 1984-05-24 1987-07-14 Damon Biotech, Inc. Induction of immune response by immunization with encapsulated antigen-producing cells
IL79052A0 (en) * 1986-06-06 1986-11-30 Univ Ramot Device and process for production of alginate-shell beads containing biologically active material
AU6571598A (en) * 1997-03-18 1998-10-12 Chromaxome Corporation Methods for screening compounds using encapsulated cells
US20120196770A1 (en) * 2011-01-28 2012-08-02 Amyris, Inc. Gel-encapsulated microcolony screening

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4352883A (en) * 1979-03-28 1982-10-05 Damon Corporation Encapsulation of biological material
US4663286A (en) * 1984-02-13 1987-05-05 Damon Biotech, Inc. Encapsulation of materials

Also Published As

Publication number Publication date
GB8428320D0 (en) 1984-12-19
IT1179899B (en) 1987-09-16
FR2564858A1 (en) 1985-11-29
GB2159171A (en) 1985-11-27
DE3509210A1 (en) 1985-11-28
DK57885A (en) 1985-11-25
SE8405105L (en) 1985-11-25
DE3509210C2 (en) 1988-03-31
DK57885D0 (en) 1985-02-07
JPS60250256A (en) 1985-12-10
NL8500451A (en) 1985-12-16
IL73344A0 (en) 1985-01-31
IT8468287A0 (en) 1984-12-28
AU3450684A (en) 1985-11-28
BE902457A (en) 1985-09-16

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Legal Events

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Effective date: 19880501