BE1030108A1 - PROPAGATION METHOD OF CUTTINGS FOR REJUVENATION OF PINE CUTTINGS - Google Patents

Abstract

The present invention provides a cutting propagation method for rejuvenating pine cuttings, comprising rejuvenation treatment, selection of cuttings, dosage of cutting propagation substrate, treatment with a home-made rooting agent, management of cuttings, and training of cuttings. through the breeding of young plants by container cuttings propagation from older mother trees of pine, after rejuvenation and treatment with rooting agents, the rooting time shortens from 50 days to 35 days, the rate of lateral root sprouting increases by 45%, and the survival rate of the cuttings reaches more than 80% compared to control with no regeneration and nursery soil cuttings propagation. The method of the present invention is simple, clear and workable, and has good stability and repeatability.

Description

PROPAGATION METHOD OF CUTTINGS FOR REJUVENATION OF PINE CUTTINGS “0923/5389

TECHNICAL AREA

The present invention relates to the technical field of plant breeding and a

Method of propagating plants by cuttings, in particular a container

Cutting propagation method for rejuvenation of pine trees.

STATE OF THE ART

The pine is a widespread type of afforestation. Due to natural factors and human felling, many good stands of pine have suffered devastating damage.

At present, propagation by cuttings is an important means of protecting and utilizing pine stands. However, the existing cuttings propagation is limited by the "maturation effect", i.e. the collected cuttings bear the physiological age of the

mother tree, which directly affects the survival rate of the cuttings and, above all, leads to a phenotypic decline in the cuttings when they reach adulthood, causing incalculable damage to the structure of the plantation. Hence the

Rejuvenation of mature trees is an effective way to restore vitality to good germplasm stocks. Most existing pine cutting propagation will be in

Nurseries conducted, and the survival rate of the cuttings is low. It is therefore necessary to improve the existing technology to counteract the decline in pine trees after the

Prevent cutting propagation and ensure the proper establishment of the plantation.

CONTENT OF THE PRESENT INVENTION

To solve the problems that the "maturation effect" of older mother trees leads to low cutting survival rate and the phenotypic decline after the

reaching adulthood, the present invention provides a method for

Disposal that realizes the rejuvenation of cuttings from older mother trees and the

Increased survival rate of cuttings.

The present invention is realized by the following technical solution: a

A method of propagation by cuttings for rejuvenating pine cuttings, comprising the

Rejuvenation treatment, the selection of the cuttings, the dosage of the

Cuttings propagation substrate, treatment with a self-made

Rooting agent, management of the cuttings and training of the cuttings, BE2023/5389 characterized as comprising the following steps:

A. Morning or late evening, healthy, semi-lignified current year cuttings are taken from lateral branches above the crown of a pine tree trunk aged 20-50

Collected years old, cut to a length of 10-12 cm. The needles are cut off from the base to 2/3 of the top, and the cuttings are wrapped in a damp towel so that they remain moist;

B. Soak the cuttings for 2-5 min in a 1000X solution of 75%

Carbendazim wetting powder, then soaking the incisions of the cuttings for 40-60 min in an IBA solution of 400-600mg/L and sticking the cuttings with the incisions down into a container by 3 cm, which contains the treated substrate with the following Volume ratio contains:

Peat soil: vermiculite: perlite = 1:1:1 where substrate is treated as follows: uniformly mixing the substrate with an appropriate amount of a 1000-fold solution of 75% carbendazim-

Wetting powder, cover and leave for 3 days, or perform one directly

High temperature sterilization for the substrate, dividing the substrate into containers with a

length of 10-15 cm, a width of 10-15 cm and a height of 12 -20cm a day before

propagation from cuttings, and thorough watering;

C. Leaving the containers after the propagation of cuttings at a temperature of 25°C, a light intensity of 6000 Lx and a humidity of 85% for 16 hours a day and at a temperature of 22°C, a light intensity of 6000 Lx and a humidity of 85% for 8 hours at night;

D. Spraying the 1000 fold solution of 75% carbendazim wetting powder every 5-7 days, and pouring the following rooting agents: 80g sucrose, 0.1g salicylic acid, adding water and bring this to 1L, mixing evenly, the interval between the application of carbendazim and rooting agent is half an hour until the

rooting cuttings;

E. After the cuttings rooted, container seedlings are transferred to the nursery, stretching a 50% shading net, watering an appropriate amount of water, keeping the soil in the containers moist, and transplanting the seedlings for afforestation after usual training for two weeks become;

F. When the cuttings are 3-6 years old, the rejuvenated cuttings are collected, and 7023/5369

Repeating steps B, C, D and E to complete the regeneration and container cutting propagation of older pine mother trees.

The present invention has the following advantages and positive effects: using the above solution for growing young plants through the container

Propagation by cuttings from older mother trees of pine shortens after

Rejuvenation and treatment with rooting agents, the rooting time from 50 days to 35 days, the rate of lateral rooting increases by 45% and the

Survival rate of cuttings reaches more than 80% compared to control without

Rejuvenation and nursery soil cutting propagation. The procedure of the present

Invention is simple, clear and easy to implement and has good stability and

repeatability up.

DETAILED DESCRIPTION

The present invention is explained in more detail below in connection with exemplary embodiments.

Example 1

A. In the morning, take healthy, semi-lignified current year cuttings from laterals

Branches are collected above the crown of a pine trunk tree 30 years old, cut to a length of 10 cm, the needles are cut off from the base to 2/3 of the top, and the cuttings are wrapped in a damp towel to keep them moist ;

B. Soak the cuttings for 2 min in a 1000X solution of 75% carbendazim-

Wetting powder, then soaking the incisions of the cuttings for 60 min in a

IBA solution of 400mg/L and insert the cuttings with the side down

Incisions in a container by 3 cm containing the treated substrate with the following

Volume ratio contains:

Peat soil: vermiculite: perlite = 1:1:1 where substrate is treated as follows: uniformly mixing the substrate with an appropriate amount of a 1000-fold solution of 75% carbendazim-

Wetting powder, cover and leave for 3 days, or perform one directly

High temperature sterilization for the substrate, dividing the substrate into containers with a

Length of 10 cm, width of 15 cm and height of 12 cm one day before BE2023/5389

propagation from cuttings, and thorough watering;

C. Leaving the containers after the propagation of cuttings at a temperature of 25°C, a light intensity of 6000 Lx and a humidity of 85% for 16 hours a day and at a temperature of 22°C, a light intensity of 6000 Lx and a humidity of 85% for 8 hours at night;

D. Spraying 1000X solution of 75% carbendazim wetting powder every 5 days and pouring the following rooting agents: 80g sucrose, 0.1g salicylic acid, adding water and bringing this to 1 L, mixing evenly, the interval between the Application of carbendazim and rooting agent is half an hour until the

rooting cuttings;

E. After the cuttings rooted, container seedlings are taken to the nursery, putting up a 50% shading net, pouring an appropriate amount of water, keeping the soil in the containers moist, and transplanting the seedlings for afforestation after usual training for two weeks become;

F. When the cuttings are 3 years old, the rejuvenated cuttings are collected, and

Repeating steps B, C, D and E to complete the regeneration and container cutting propagation of older pine mother trees.

The survival rate of the cuttings is more than 80%, while the survival rate of non-rejuvenated cuttings in the prior art is less than 50%.

Example 2

A. In the morning, take healthy, semi-lignified current year cuttings from laterals

Branches are collected above the crown of a pine trunk tree with an age of 40 years, cut to a length of 12 cm, the needles are cut off from the base to 2/3 of the top, and the cuttings are wrapped in a damp towel so that they are moist remain;

B. Soak the cuttings for 5 min in a 1000X solution of 75% carbendazim-

Wetting powder, then soaking the incisions of the cuttings for 40 min in a

IBA solution of 600 mg/L and insert the cuttings with the side down

Incisions in a container by 3 cm containing the treated substrate with the following

Volume ratio contains:

Peat soil:vermiculite:perlite = 1:1:1 where substrate is treated as follows: uniformly mixing the substrate with an appropriate amount of BE2023/5389 a 1000X solution of 75% carbendazim

Wetting powder, cover and leave for 3 days, or perform one directly

High-temperature sterilization for the substrate, dividing the substrate into containers with a length of 15 cm, a width of 15 cm and a height of 12 cm one day before the

propagation from cuttings, and thorough watering;

C. Leaving the containers after the propagation of cuttings at a temperature of 25°C, a light intensity of 6000 Lx and a humidity of 85% for 16 hours a day and at a temperature of 22°C, a light intensity of 6000 Lx and a humidity of 85% for 8 hours at night;

D. Spraying 1000X solution of 75% carbendazim wetting powder every 7 days and pouring the following rooting agents: 80g sucrose, 0.1g salicylic acid, adding water and bringing this to 1 L, mixing evenly, the interval between the Application of carbendazim and rooting agent is half an hour until the

rooting cuttings;

E. After the cuttings rooted, container seedlings are transferred to the nursery, stretching a 50% shading net, watering an appropriate amount of water, keeping the soil in the containers moist, and transplanting the seedlings for afforestation after usual training for two weeks become;

F. When the cuttings are 6 years old, the rejuvenated cuttings are collected, and

Repeating steps B, C, D and E to complete the regeneration and container cutting propagation of older pine mother trees.

The survival rate of the cuttings is more than 85%, while the survival rate of non-rejuvenated cuttings in the prior art is less than 50%.

Example 3

A. Morning healthy, semi-lignified current year cuttings from lateral

Branches are collected above the crown of a pine tree with an age of 50 years, cut to a length of 11 cm, the needles are cut off from the base to 2/3 of the top, and the cuttings are wrapped in a damp towel so that they are moist remain;

B. Soak the cuttings for 3 min in a 1000X solution of 75% carbendazim-

Wetting powder, then soaking the incisions of the cuttings for 50 min in a

IBA solution of 500 mg/L and insert the cuttings with the side down

Cut 3 cm into a container containing the treated substrate with the following BE2023/5389

Volume ratio contains:

Peat soil: vermiculite: perlite = 1:1:1 where substrate is treated as follows: uniformly mixing the substrate with an appropriate amount of a 1000-fold solution of 75% carbendazim-

Wetting powder, cover and leave for 3 days, or perform one directly

High temperature sterilization for the substrate, dividing the substrate into containers with a

Length of 12 cm, a width of 12 cm and a height of 12cm a day before

propagation from cuttings, and thorough watering;

C. Leaving the containers after the propagation of cuttings at a temperature of 25°C, a light intensity of 6000 Lx and a humidity of 85% for 16 hours a day and at a temperature of 22°C, a light intensity of 6000 Lx and a humidity of 85% for 8 hours at night;

D. Spraying 1000X solution of 75% carbendazim wetting powder every 6 days and pouring the following rooting agents: 80g sucrose, 0.1g salicylic acid, adding water and bringing it to 1 L, mixing evenly, noting the interval between the Application of carbendazim and rooting agent is half an hour until the

rooting cuttings;

E. After the cuttings rooted, container seedlings are transferred to the nursery, stretching a 50% shading net, watering an appropriate amount of water, keeping the soil in the containers moist, and transplanting the seedlings for afforestation after usual training for two weeks become;

F. When the cuttings are 4 years old, the rejuvenated cuttings are collected, and

Repeating steps B, C, D and E to complete the regeneration and container cutting propagation of older pine mother trees.

The survival rate of the cuttings is more than 82%, while the survival rate of non-rejuvenated cuttings in the prior art is less than 50%.

Claims (1)

CLAIMS BE2023/5389 A cuttings propagation method for rejuvenating pine cuttings, comprising the rejuvenation treatment, the selection of the cuttings, the dosage of the cuttings propagation substrate, the treatment with a home-made rooting agent, the management of the cuttings and the training of the cuttings, characterized in that it comprises the following steps: A. In the morning or towards the evening, healthy semi-lignified cuttings of the current year are collected from lateral branches above the crown of a pine trunk tree 20-50 years old, cut to a length of 10-12 cm, the needles from the base up to 2 /3 of the top are cut off, and the cuttings are wrapped in a damp towel to keep them moist; B. Soaking the cuttings for 2-5 min in a 1000X solution of 75% carbendazim wetting powder, then soaking the incisions of the cuttings for 40-60 min in an IBA solution of 400-600mg/L and plugging the cuttings with the incisions pointing downwards, into a container by 3 cm, containing the treated substrate with the following volume ratio: peat soil:vermiculite:perlite = 1:1:1 substrate being treated as follows: uniformly mixing the substrate with an appropriate amount of a 1000-fold solution of 75% carbendazim wetting powder, cover and leave for 3 days, or directly conduct high-temperature sterilization for the substrate, dividing the substrate into containers 10-15 cm long, 10-10 cm wide 15 cm and a height of 12-20 cm a day before the cuttings propagation, and watering thoroughly; C. Leaving the containers after the propagation of cuttings at a temperature of 25°C, a light intensity of 6000 Lx and a humidity of 85% for 16 hours a day and at a temperature of 22°C, a light intensity of 6000 Lx and a humidity of 85% for 8 hours at night; D. Spraying the 1000 fold solution of 75% carbendazim wetting powder every 5-7 days, and pouring the following rooting agents: 80g sucrose, 0.1g salicylic acid, adding water and bring this to 1L, mixing evenly, the interval half an hour between application of carbendazim and rooting agent until the cuttings take root; E. After cuttings have rooted, container seedlings are transferred to nursery PE2029/5989, set up 50% shading net, water appropriate amount of water, keeping soil in containers moist, and seedlings after usual training for two weeks be transplanted for afforestation; F. When the cuttings are 3-6 years old, the rejuvenated cuttings are collected, and repeating steps B, C, D and E to complete the rejuvenation and container cuttings propagation from older mother trees of pine.