BE1029878B1 - Biofermentation agent for composting cattails, process for its production and use - Google Patents

Abstract

The present invention discloses a biofermentative for composting cattails, a method for its production, the biofermentative comprises Bacillus subtilis, Bacillus belleus, Corynebacterium glutamicum, Trichoderma viride and Pseudomonas stutzeri. The composite bacterial agent of the present invention has a strong combination ability, synergistic ability and complementarity, which can coordinate with each other in the compost, increasing the fermentation temperature quickly and keeping it at a high temperature for a long time, which promotes the decomposition of cattail plants, and whereby high levels of nutrients such as organic matter can be obtained after composting, by promoting efficient nutrient cycling in agricultural aquatic ecosystems, composting can effectively improve growth and productivity.

Description

" BE2022/5783

Biofermentation agent for composting cattails, method for its

manufacture and use

TECHNICAL AREA

The present invention relates to the field of microbial

Fermentation technology, in particular a biofermentative for composting cattails, a process for its production and use.

STATE OF THE ART

So far, the resource use research of cattails is mainly the

Paper production, medical use, production of silage, production of organic fertilizer. However, such cannot easily be extended in practical use, e.g. B. No precise and workable technical solutions are in the process of

Production of organic fertilizer from cattails. Furthermore, the existing

Technology a long fermentation time, complex operation, low fertilizer nutrients and other problems that result in bad fertilizer.

For this reason it is important to find a simpler, cheaper and more effective

Biofermentation agent for composting cattails and a method for its

provide manufacturing.

CONTENT OF THE PRESENT INVENTION

In view of the above description, the present invention establishes

Biofermentative for composting cattails, a process for its

Ready to manufacture as well as use to effectively utilize the aquatic plant waste and promote efficient nutrient cycling in terrestrial-aquatic ecosystems, where composting cattails can effectively improve the growth and yield of saline plants.

In order to achieve the above object, the following technical solutions are used in the present invention:

A biofermentative for composting cattails, comprising the composite germs: Bacillus subtilis, Bacillus belleus, Corynebacterium glutamicum, Trichoderma viride and Pseudomonas stutzeri.

Preferably, the composite seed includes the following mass fractions

Raw materials: Bacillus subtilis 25 - 35%, Bacillus belleus 15 - 25%, Corynebacterium glutamicum 15 - 25%, Trichoderma viride 15 - 25% and Pseudomonas stutzeri 15 - 25%.

Preferably, the composite seed includes the following mass fractions

Raw materials: Bacillus subtilis 30%, Bacillus belleus 20%, Corynebacterium glutamicum 20%,

Trichoderma viride 10% and Pseudomonas stutzeri 20%.

° BE2022/5783

Preferably the Bacillus subtilis has a colony forming unit (CFU) of 0.5 -1 x 10° cfu/g, the Bacillus belleus a colony forming unit (CFU) of 0.05 - 0.15 x 10° cfu/g, the

Corynebacterium glutamicum a colony-forming unit (CFU) of 0.05 - 0.15 x 10° cfu/g, the Trichoderma viride a colony-forming unit (CFU) of 0.03 - 0.09 x 10° cfu/g, whereby

Pseudomonas stutzeri has a colony forming unit (CFU) of 0.01 - 0.05 x 10° cfu/g; with the total active live bacteria being 0.65 - 1.5 x 10° cfu/g.

Preferably, it further comprises an excipient and a soluble starch, the combined germ, excipients and soluble starch having a mass fraction of (10-30%):(10-20%):(20-70%).

Preferably, the combined germ, excipients and soluble starch have a mass fraction of 30%:20%:50%.

The excipient is preferably a mixture of protein powder,

pullulan polysaccharide and humic acid in the mass ratio of (3-6):(2-3):(0.2-1), and the soluble starch comprises one of the group consisting of rice, corn, potato and yams.

The dosage excipients in the present invention can improve the stability of the compound bacterial agent, enhance the effect of the compound bacterial agent, and have strong plasticity and solubility.

The excipient is preferably a mixture of protein powder,

Pullulan polysaccharide and humic acid in a mass ratio of 5:2.5:0.8.

Process for producing a biofermentative for composting

Cattails as described above, comprising the following specific steps: (1) Weighing raw materials by mass percentage of Bacillus subtilis, Bacillus belleus,

Corynebacterium glutamicum, Trichoderma viride, Pseudomonas stutzeri, the excipient and the soluble starch; (2) Inoculation of Bacillus subtilis, Bacillus belleus, Corynebacterium glutamicum,

Trichoderma viride, Pseudomonas stutzeri into the culture medium to obtain a stock solution of the assembled germs, then the excipients and soluble starch are added one by one and mixed well to obtain a biofermentative for

Obtain composting cattails.

Preferably, the culture medium contains: peptone 5-10 g/l, glucose 1.0-1.5 g/l, potato 100-200 g/l, NaCl 2-5 g/l, KH2PO4 1.0-1.5 g /l, MgSO4:7H20 0.5 - 1.0 g/l.

Preferably, the culture medium contains: peptone 10 g/l, glucose 1.25 g/l, potato 200 g/l (with leaching juice), NaCl 5 g/l, KH2PO4 1.5 g/l, MgSO4:7H2O 0.5 g /l.

Preferably the medium has a pH of 5-7, preferably 6.8.

The mass percentage of the inoculation is preferably 4-8%, the incubation takes place under incubation conditions of 32-37° C. for 12-24 h and 150-200 rpm shaking speed.

The incubation conditions are preferably 6°C for 18 hours and one

Shaker speed of 180 rpm.

° BE2022/5783

Use of the biofermentative agent described above or the biofermentative agent prepared as described above in the composting of cattails.

Preferably, the method of fermenting the compost of cattails such as

Following: (1) Calculate Each material in parts by weight: 800 to 860 parts by weight

cattails, 140 to 200 parts by weight of sheep or chicken manure, 70 to 100 parts by weight of urea and 0.5 to 1.5 parts by weight of the biofermentation agent; (2) Chopping the harvested and air-dried cattail after removing the

Residues with the help of shredders to less than 5 cm; then the air-dried and crushed cattail is mixed with sheep or chicken manure and

Urea is added to the mixture at a ratio of 20:1 to 25:1

adjust carbon/nitrogen; then the biofermentation agent is added to the above mixture and the water content is adjusted to 60-70% by adding water; (3) Stacking the heap after adjusting the carbon-nitrogen ratio and the water content of the mixture of heaps into a cone or trapezoidal heap, being in the high-temperature composting, according to the actual situation of

Ambient temperature, the tarpaulin and non-woven fabric can be used to cover and keep the humidity and temperature; where the high temperature is 50 to 75°C, when the central temperature of the heap is higher than 70°C, the heap is rotated and supplemented with water to ensure that the moisture content of the heap is kept at about 60%; in the first phase the heap is turned every 3 to 5 days, and after 15 days turned every 7 to 10 days and stored for a total of 35 to 45 days.

The organic substance content of the cattail compost produced by the process according to the invention is 25-30%, the total nitrogen content is 2.2-2.8%, the total phosphorus content is 0.6-1.2% and the total potassium content is 1.4-2.0% what dem

is superior to the prior art.

Cattail compost is preferably used to improve saline soils.

Cattail compost is preferably used in plant cultivation.

As can be seen from the above technical solutions, the present invention

Compared to the prior art, the following advantageous effects. (1) The composite bacterial agent of the present invention has a strong

Combining ability, synergistic ability and complementarity, which can coordinate with each other in the compost — with the fermentation temperature increasing rapidly and keeping at a high temperature for a long time, which promotes the decomposition of cattail plants, and with a high content of nutrients such as organic matter after

composting can be obtained;

* BE2022/5783 (2) the biofermentative agent of the present invention can promote plant growth and prevent the occurrence of pests and diseases; (3) The biofermentation agent of the present invention can be in a powder form and can be completely soluble in water, the method of use is simple and diversified.

BRIEF DESCRIPTION OF THE DRAWINGS

Figure 1 shows the change curve of the temperature of the heap from the

Application examples 1 - 3 of the present invention.

DETAILED DESCRIPTION

embodiments

example 1

The specific steps of a process for producing a biofermentative for composting cattails are as follows. (1) Preparation of the culture medium: peptone (10 g/l), glucose (1.25 g/l), potato (200 g/l) (with leaching juice), NaCl (5 g/l), KH2PO«4 (1 .5 g/l), MgSO4:7H2O (0.5 g/l), water 1000 ml, pH 6.8;

Preparation of a composite bacterial agent: the percentage by weight of the

Mixture of germs in the composite bacterial agent is: Bacillus subtilis:

Bacillus belleus: Corynebacterium glutamicum: Trichoderma viride: Pseudomonas stutzeri = 30%: 20%: 20%: 10%: 20%; wherein the colony forming unit (CFU) of Bacillus subtilis is 0.5 X 109 cfu/g, the colony forming unit (CFU) of Bacillus belleus is 0.06 X 109 cfu/g, the colony forming unit (CFU) of Corynebacterium glutamicum is 0, 05 X 109 cfu/g, the colony forming unit (CFU) of Trichoderma viride was 0.05 X 109 cfu/g and the colony forming unit (CFU) of Pseudomonas stutzeri is 0.02 X 109 cfu/g, the total effective live bacteria are 0.68 X 109 cfu/g.

Preparation of excipients: The mixture weight percentage of the compositions in the excipients is: protein powder: pullulan polysaccharide: humic acid = 5.5:2:0.6; the

Weight percentage of soluble starch such as potato starch and water is 20%:80%;

The weight percentage of the composite bacterial agent, adjuvants and soluble starch is 20%:20%:60%. (2) The compound sprouts are inoculated at 5% by weight into the medium with bed shaking at 180 rpm and incubation is carried out at 36°C for 18 hours to obtain the compound sprouts stock solution; then they will

Stock solution of the compound germ, the adjuvants and the soluble starch are uniformly mixed to obtain a biofermentative for composting cattails.

° BE2022/5783

example 2

The specific steps of a process for producing a biofermentative for composting cattails are as follows. (1) Preparation of culture medium: as in Example 1.

Preparation of a composite bacterial agent: the percentage by weight of the

Mixture of germs in the composite bacterial agent is: Bacillus subtilis:

Bacillus belleus: Corynebacterium glutamicum. Trichoderma viride: Pseudomonas stutzeri = 30%: 20%: 20%: 10%: 20%; wherein the colony forming unit (CFU) of Bacillus subtilis is 0.6 X 10°cfu/g, the colony forming unit (CFU) of Bacillus belleus is 0.08 X 10° cfu/g, the colony forming unit (CFU) of Corynebacterium glutamicum is 0.06 x 10°cfu/g, the colony forming unit (CFU) of Trichoderma viride was 0.06 x 10°cfu/g and the colony forming unit (CFU) of Pseudomonas stutzeri is 0.03 x 10°cfu/g , where the total effective live bacteria is 0.83 x 10°cfu/g.

Preparation of excipients: The mixture weight percentage of the compositions in the excipients is: protein powder: pullulan polysaccharide: humic acid = 5:2.5:0.8; the

Weight percentage of soluble starch such as potato starch and water is 25%:75%;

The weight percentage of the composite bacterial agent, adjuvants and soluble starch is 30%:20%:50%. (2) The compound sprouts are inoculated at 6% by weight into the medium with bed shaking at 180 rpm and incubation is carried out at 36°C for 18 hours to obtain the stock solution of the compound sprouts; then they will

Stock solution of the compound germ, the adjuvants and the soluble starch are uniformly mixed to obtain a biofermentative for composting cattails.

Example 3

The specific steps of a process for producing a biofermentative for composting cattails are as follows. (1) Preparation of culture medium: as in Example 1.

Preparation of a composite bacterial agent: the percentage by weight of the

Mixture of germs in the composite bacterial agent is: Baci//us subtilis:

Bacillus belleus: Corynebacterium glutamicum: Trichoderma viride: Pseudomonas stutzeri = 30% : 20% : 20% : 10% : 20%; wherein the colony forming unit (CFU) of Bacillus subtilis is 0.7 X 10°cfu/g, the colony forming unit (CFU) of Bacillus belleus is 0.08 X 10°cfu/g, the colony forming unit (CFU) of Corynebacterium glutamicum is 0.08 x 10°cfu/g, the colony forming unit (CFU) of Trichoderma viride was 0.06 x 10°cfu/g and the colony forming unit (CFU) of Pseudomonas stutzeri is 0.03 x 10% cfu/g , where the total effective live bacteria is 0.95 x 10°cfu/g.

° BE2022/5783

Preparation of excipients: The mixture weight percentage of the compositions in the excipients is: protein powder: pullulan polysaccharide: humic acid = 4.8:2.2:1; the

weight percentage of soluble starch such as potato starch and water is 25% : 75%;

The weight percentage of the composite bacterial agent, adjuvants and soluble starch is 30%:10%:60%. (2) The compound sprouts are inoculated at 8% by weight into the medium with bed shaking at 180 rpm and incubation is carried out at 36°C for 18 hours to obtain the stock solution of the compound sprouts; then they will

Stock solution of the compound germ, the adjuvants and the soluble starch are uniformly mixed to obtain a biofermentative for composting cattails.

Application example 1

The application method of the biofermentative in Example 1 for composting cattails, comprising the following steps: (1) providing the materials in parts by weight: 850 parts of cattails, 180 parts of sheep manure, 85 parts of urea, and 0.8 parts of the biofermentative; (2) Chopping the harvested and air-dried cattail after removing the

Residues with the help of shredders to less than 5 cm; then the air-dried and crushed cattail is mixed with sheep or chicken manure and

added urea to adjust the mixture to a 25:1 carbon/nitrogen ratio; then the biofermentative is added to the above mixture and the water content is adjusted to 60% by adding water; (3) Stacking the heap into a cone after adjusting the carbon-nitrogen ratio and the water content of the mixture of heap with the tonneau cover

cover and keeping the humidity and temperature used; if the central

If the temperature of the heap is higher than 70 °C, the heap is turned over and water is added to ensure that the moisture content of the heap is kept at 60%; in the first phase the heap is turned every 5 days, and after 15 days turned every 10 days and stored for a total of 45 days. The resulting compost has an organic matter content of 28.1%, a total nitrogen content of 2.52%, a

0.97% total phosphorus and 1.88% total potassium.

Application example 2

The application method of the biofermentative in Example 2 for composting cattails, comprising the following steps: (1) Providing the materials in parts by weight: 800 parts of cattails, 150 parts of

chicken manure, 80 parts of urea and 0.5 parts of the biofermentative;

/ BE2022/5783 (2) Adjusting the carbon/nitrogen ratio to 24:1 and the water content of the

mixture to 65%; (3) Maintaining the water content of the heap at about 70%.

All other steps are the same as in application example 1.

The resulting compost has an organic matter content of 25.3%, a

total nitrogen content of 2.34%, a total phosphorus content of 0.66% and a

Total Potassium Content of 1.52%.

Application example 3

The application method of the biofermentative in Example 3 for composting cattails, comprising the following steps: (1) Providing the materials in parts by weight: 860 parts of cattails, 180 parts of

sheep manure, 90 parts of urea and 1.2 parts of the biofermentant; (2) Adjust the carbon/nitrogen ratio to 22:1 and the water content of the

Mix to 70%. (3) maintaining the water content of the heap at about 70%; where the heap in the first

phase is turned once every 3 days and after 15 days of composting once every 7 days, for a total of 43 days;

All other steps are the same as in application example 1.

The resulting compost has an organic matter content of 27.5%, a

total nitrogen content of 2.49%, a total phosphorus content of 0.73% and a

Total Potassium Content of 1.66%.

In the above application examples 1 - 3 for compost treatment, every 3 days before turning at 15:00 to determine the temperature of the heap with a thermometer, take the periphery of the heap and the center as the measuring point, the measuring point being a

depth of 25 cm, take the average of 5 points as the temperature of the

heaps, the corresponding temperature changes is shown in Fig.1, from which it can be seen that the compound bacterial agent promotes the decomposition of cattail plants, and the fermentation temperature increases rapidly and keeps at a high temperature for a long time, with the treatment im Use case 1 is relatively better.

Application example 4

Antagonistic experiments with different bacterial strains.

Six bacterial strains screened out are placed in two crossed lines on sodium

Carboxymethyl cellulose medium inoculated and incubated at 37 °C constant temperature.

Then, the medium is observed to see if colony growth can occur at the area where different strains of bacteria cross. If a colony growth is seen intact, it means that no antagonism between the strains is present

° BE2022/5783 is thus able to carry out the preparation of composite bacterial agents that

Results are shown in Table 1.

A: Bacillus subtilis; B: Bacillus belleus; C: Corynebacterium glutamicum, D: Trichoderma viride, E: Pseudomonas stutzeri, F: Lactobacillus acidophilus

The results in Table 1 show that the strains are not antagonistic to each other, with the exception of À and F, which are antagonistic to each other.

Table 1 Results of antagonism experiments with 6 strains

tribes) A | B] 81DL EL FF)

LOUD)

LB UT T4) 5LT TT JT

LB

LE TT TT TN)

FL LLL LL

Note: + means an antagonistic reaction, - means no antagonistic

Reaction.

Application example 5

Investigation of the effect of different inoculum amounts on the degradation of

Cattails using a culture experiment.

Preparation of the culture medium: as in example 1.

The medium is supplemented with a composite germ (Bacillus subtilis 30%, Bacillus belleus 20%, Corynebacterium glutamicum 20%, Trichoderma viride 10% and Pseudomonas stutzeri 20%) in proportions by weight of 1%, 3%, 5%, 7% and 9% inoculated. After three days

Incubation at 36 °C on a 180 rpm shaker is put in 10 g of sterilized cattails and after 15 days of rest sampling is carried out. The

Rates of degradation of lignin, hemicellulose and cellulose are measured; the

Results are listed in Table 2.

Table 2: Effect of different inoculum amounts on the degrading effect of cattails 1% 1% 1% po # | #| æ | -po$| #| | - pu Ÿ | | & | =

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Application example 6

Investigation of the effect of different inoculation times on the degradation of

Cattails using a culture experiment.

Preparation of the culture medium: as in example 1.

The medium is mixed with a sprouting compound (as in Example 5) in

Weight proportions of 5% inoculated. After three days of incubation at 36 °C on a 180 rpm shaker, 10 g of sterilized cattails are put in, and after 1, 5, 10, 15 and 20 days

Sampling is carried out during the rest period. The rate of degradation of lignin, hemicellulose and cellulose are measured; the results are shown in Table 3. From the table it can be seen that a period of 15 days is optimized for cattail degradation effect.

Table 3: Effect of different inoculation times on cattail degradation 1% 1% 1%

P.T. | | #4 pu 5 | #1] w | -

Application example 7

Investigation of the effect of various monogerms and compound germs on the breakdown of cattails by means of a culture experiment.

Preparation of the culture medium: as in example 1.

A total of 10 treatments, 5 monosprout and 5 compound sprouts.

A: Bacillus subtilis; B: Bacillus belleus; C: Corynebacterium glutamicum, D: Trichoderma viride; E: Pseudomonas Stutzer.

Z1: Bacillus subtilis 30%, Bacillus belleus 20%, Corynebacterium glutamicum 20%,

Trichoderma viride 10% and Pseudomonas stutzeri 20%.

Z2: Bacillus subtilis 25%, Bacillus belleus 25%, Corynebacterium glutamicum 20%, - Trichoderma viride 15% and Pseudomonas stutzeri 15%.

Z3: Bacillus subtilis 35%, Bacillus belleus 15%, Corynebacterium glutamicum 25%,

Trichoderma viride 5% and Pseudomonas stutzeri 20%.

ZA: Bacillus subtilis 30%, Bacillus belleus 15%, Corynebacterium glutamicum 15%,

Trichoderma viride 15% and Pseudomonas stutzeri 25%.

Z5: Bacillus subtilis 30%, Bacillus belleus 20%, Corynebacterium glutamicum 25%,

Trichoderma viride 10% and Pseudomonas stutzeri 15%.

The medium is mixed with various mono - and compound germs in

Weight proportions of 5% inoculated. After 3 days of incubation at 36 °C on a shaker at 180 rpm, 10 g of sterilized cattails are put in, and after 15 days of rest, they are used

sampling performed. The rate of degradation of lignin, hemicellulose and cellulose are measured; the results are shown in Table 4.

From the results in Table 4, it can be seen that the above five bacteria have a high synergistic effect and the composite bacterial agent of the present invention has a very significant cattail decomposing effect, with the composite strain having the composition Z1 having the best cattail decomposing effect has cattails.

Table 4 Effect of various mono - and compound germs on the

Decomposition effect of cattails 1% 1%

PUR | 9 | mæ | -

PM u8 | ## | w# [me | #9 |;

PP | 8 | 84 | -

FE [Le

La A

Application example 8

In a pot experiment, the effect of cattail compost mori on the growth of wheat on saline soils is examined.

A control group and an experimental group are formed, with a saline soil not mixed with compost as a control group 1, and a flower pot mixture of a one prepared by commercially available BM straw decomposition (Henan Baorong Biotechnology Co., Ltd.).

Cattail compost and brine in a ratio of 1:9 as control group 2.

The compost prepared from the biofermentation agent in Examples 1 to 3 is mixed with the brine in a ratio of 1:9 and filled into the flowerpots for treatments 1 to 3, with three for both the control and experimental groups

repetitions are set.

" BE2022/5783

After the soil is mixed with the compost, the experimental

Soil samples packed in pots, each pot contained about 3 kg of soil samples, and the pots are marked after filling. 15 wheat seeds are planted in each pot, and watered regularly every day, after the wheat seedlings grow, the

Interplanted seedlings leaving 10 seedlings with the same growth potential. The plants are harvested after 25 days of growth and the height, root length,

Leaf width and biomass (dry weight) of the wheat plants are measured, and the

Results are shown in Table 5.

Table 5 Effect of compost on wheat biomass

Treatment seedlings mm [ee Se

As can be seen from Table 5, the height of the seedlings and biomass are the

Wheat plants in the improved compost treatment significantly higher than in the

Control group 1. There is no significant difference in the effect of biofermentation-

Composting of the test groups 1 - 3 and the commercial BM straw decomposition

Composting of the control group 2 on the height and biomass of the wheat, the

Experimental group 2 has the relatively best effect.

Application example 9

In a field test, the effect of cattail compost of test group 2 on the yield of rice in saline farmland is examined.

The organic matter content is 28.1%, the total nitrogen content is 2.52%, the total phosphorus content is 0.97% and the total potassium content is 1.68% in the

Cattail compost of experimental group 2.

The tentatively saline farmland has a pH of 8.45 and a

Total salinity of 0.36 g/kg in the soil layer from 0 -20 cm, therefore it belongs to moderately saline soils.

A part of the chemical nitrogen fertilizer is made by the cattail compost

Experimental Group 2 is substituted as the base fertilizer, and it is evenly applied to the surface layer of the tilled soil by the sprinkling method and worked several times to mix well with the cultivated soil; the corresponding results are shown in Table 6.

Table 6 Effect of Compost on Rice Yield; thousand kom weight

Treatment

Chemical | com- ;

Poire | | Pre Ge | 00 | 0 | ge

fertilizer post

rx | 9 10] 0 | ## | 0 | FS | mon. naw | | 0 | 0 | we | 7e | 5 | from ne | 0 60 | 300 | 20887 | 18:96 | 2889 | 5e)

From the table above it can be seen that the combination of the data

Cattail compost with nitrogen fertilizer has a trend to improve the grain count of rice ear, and thus the rice yield, the effect is relatively better when the

Cattail compost replaced 40% by chemical nitrogen fertilizer, and applied in an amount of about 300 kg/are.

The above description of the disclosed embodiments allows for this

skilled in the art to make or use the present invention. A multitude of

Modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Accordingly, the

This invention is not limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles disclosed herein and new ones

characteristics is compatible.

Claims (10)

EXPECTATIONS A biofermentative for composting cattails, comprising a combination of the following microorganisms: Bacillus subtilis, Bacillus belleus, Corynebacterium glutamicum, Trichoderma viride and Pseudomonas stutzeri. 2. Biofermentative for composting cattails according to claim 1, wherein the combination of microorganisms comprises the following mass fractions: Bacillus subtilis 25-35%, Bacillus belleus 15-25%, Corynebacterium glutamicum 15-25%, Trichoderma viride 15-25% and Pseudomonas Stutzeri 15 - 25%. 3. Biofermentative for composting cattails according to claim 1 or 2, further comprising an adjuvant and a soluble starch, wherein the combination of the microorganisms, the adjuvants and the soluble starch has a mass fraction of (10-30%):(10-20% ) : (20 - 70%) have. 4. Biofermentative for composting cattails according to claim 3, wherein the adjuvant is a mixture of protein powder, pullulan polysaccharide and humic acid in a mass ratio of (3-6):(2-3):(0.2-1) and wherein the soluble starch comprises one from the group consisting of rice, corn, potato and sweet potatoes. 5. A method for producing a biofermentative for composting cattails according to any one of claims 1 to 4, which method comprises the steps of: (1) weighing raw materials by mass percentage of Bacillus subtilis, Bacillus belleus, Corynebacterium glutamicum, Trichoderma viride, Pseudomonas stutzeri, the excipient and the soluble starch; (2) Inoculating Bacillus subtilis, Bacillus belleus, Corynebacterium glutamicum, Trichoderma viride, Pseudomonas stutzeri into a culture medium to obtain a stock solution of the combination of the microorganisms, then the excipients and soluble starch are added one by one and mixed well to obtain the biofermentation agent to obtain for composting cattails. 6. The method for producing a biofermentative for composting cattails according to claim 5, wherein the culture medium contains: peptone 5-10 g/l, glucose 1.0-1.5 g/l, potato 100-200 g/l, NaCl 2 -5 g/l, KH2PO4 1.0 - 1.5 g/l, MgSO4-7H20 0.5 - 1.0 g/l. 7. The method for producing a biofermentative for composting cattails according to claim 5, wherein the mass percentage of inoculation is 4-8% and the incubation is carried out at incubation conditions of 32-37°C for 12-24 hours. 8. Use of a biofermentation agent according to any one of claims 1-4 or a biofermentation agent prepared according to any one of claims 5-7 in the composting of cattails. 9. Use of composting cattails according to claim 8 for improving saline soil. 10. Use of composting cattails according to claim 8 in crop production.