AU770915B2 - Enterococcus faecalis polynucleotides and polypeptides - Google Patents

Description

P/00/011 28/5/91 Regulation 3.2

AUSTRALIA

Patents Act 1990

ORIGINAL

COMPLETE SPECIFICATION STANDARD PATENT Name of Applicant: Actual Inventors: Address for service is: Human Genome Sciences, Inc.

Charles A KUNSCH Gil H CHOI Camella BAILEY Alex HROMOCKYJ WRAY ASSOCIATES 239 Adelaide Terrace Perth, WA 6000 Attorney code: WR Invention Title: "Enterococcus faecalis Polynucleotides and Polypeptides" This application is a divisional application by virtue of Section 39 of Australian Patent Application 72793/98 filed on 4 May 1998.

The following statement is a full description of this invention, including the best method of performing it known to me:- 1/2 Enterococcus faecalis polynucleotides and polypeptides Field of the Invention The present invention relates to novel Enterococcusfaecalis genes faecalis) nucleic acids and polypeptides. Also provided are vectors, host cells and recombinant methods for producing the same. Further provided are diagnostic methods for detecting Enterococcusfaecalis using probes, primers, and antibodies to the E. faecalis nucleic acids and polypeptides of the present invention. The invention further relates to screening methods for identifying agonists and antagonists of E. faecalis lo polypeptide activity and to vaccines using E. faecalis nucleic acids and polypeptides.

Background of the Invention Enterococci have been recognized as being pathogenic for humans since the turn of the century when they were first described by Thiercelin in 1988 as microscopic organisms. The genus Enterococcus includes the species Enterococcus faecalis or E. faecalis which is the most common pathogen in the group, accounting for 90 percent of all enterococcal infections. See Lewis et al. (1990) Eur J. Clin Microbiol Infect Dis.9: 111-117.

The incidence of enterococcal infections has increased in recent years and enterococci are now the second most frequently reported nosocomial pathogens.

Enterococcal infection is of particular concern because of its resistance to antibiotics.

Recent attention has focused on enterococci not only because of their increasing role in nosocomial infections, but also because of their remarkable and increasing resistance to antimicrobial agents. These factors are mutually reinforcing since resistance allows enterococci to survive in an environment in which antimicrobial agents are heavily used; the hospital setting provides the antibiotics which eliminate or suppress susceptible bacteria, thereby providing a selective advantage for resistant organisms, and the hospital also provides the potential for dissemination of resistant enterococci via the usual routes of hand and environmental contamination.

Antimicrobial resistance can be divided into two general types, inherent or intrinsic property and that which is acquired. The genes for intrinsic resistance, like other species characteristics, appear to reside on the chromosome. Acquired resistance results from either a mutation in the existing DNA or acquisition of new DNA. The various inherent traits expressed by enterococci include resistance to semisynthetic penicillinase-resistant penicillins, cephalosporins, low levels of aminoglycosides, and low levels of clindamycin. Examples of acquired resistance include resistance to chloramphenicol, erythromycin, high levels of clindamycin, tetracycline, high levels of aminoglycosides, penicillin by means of penicillinase, fluoroquinolones, and vancomycin. Resistance to high levels of penicillin without penicillinase and resistance to fluoroquinolones are not known to be plasmid or transposon mediated and presumably are due to mutation(s).

Although the main reservoir for enterococci in humans is the gastrointestinal tract, the bacteria can also reside in the gallbladder, urethra and vagina.

15 E. faecalis has emerged as an important pathogen in endocarditis, bacteremia, urinary tract infections (UTIs), intraabdominal infections, soft tissue infections, and neonatal sepsis. See Lewis et al. (1990) supra.. In the 1970s and 1980s enterococci became finnly established as major nosocomial pathogens. They are now the fourth leading cause of hospital-acquired infection and the third leading cause of bacteremia in the United States. Fatality ratios for enterococcal bactermia range from 12% to 68%, with death due to enterococcal sepsis in 4 to 50% of these cases. See T.G. Emori (1993) Clin. Microbiol. Rev. 6:428-442.

The ability of enterococci to colonize the gastrointestinal tract, plus the many intrinsic and acquired resistance traits, means that these organisms, which usually seem to have relatively low intrinsic virulence, are given an excellent opportunity to become secondary invaders. Since nosocomial isolates of enterococci have displayed resistance to essentially every useful antimicrobial agent, it will likely become increasingly difficult to successfully treat and control enterococcal infections.

Particularly when the various resistance genes come together in a single strain, an event almost certain to occur at some time in the future.

The etiology of diseases mediated or exacerbated by Enterococcus faecalis, involves the programmed expression ofE. faecalis genes, and that characterizing these genes and their patterns of expression would dramatically add to our understanding of the organism and its host interactions. Knowledge of the E.faecalis gene and genomic organization would improve our understanding of disease etiology and lead to improved and new ways of preventing, treating and diagnosing diseases. Thus, there is a need to characterize the genome of E. faecalis and for polynucleotides of this organism.

Summary of the Invention The present invention provides for isolated E. faecalis polynucleotides and polypeptides shown in Table 1 and SEQ ID NO: 1 through SEQ ID NO:496 15 (polynucleotide sequences having odd SEQ ID NOs and polypeptide sequences having even SEQ ID NOs). One aspect of the invention provides isolated nucleic acid molecules comprising polynucleotides having a nucleotide sequence selected from the group consisting of: a nucleotide sequence shown in Table 1; a nucleotide :e sequence encoding any of the amino acid sequences of the polypeptides shown in Table 1; and a nucleotide sequence complementary to any of the nucleotide sequences in or The invention further provides for fragments of the nucleic S. acid molecules of(a), above.

Further embodiments of the invention include isolated nucleic acid molecules that comprise a polynucleotide having a nucleotide sequence at least 90% identical, and more preferably at least 95%, 96%, 97%, 98% or 99% identical, to any of the nucleotide sequences in or above, or a polynucleotide which hybridizes under stringent hybridization conditions to a polynucleotide in or above.

Additional nucleic acid embodiments of the invention relate to isolated nucleic acid molecules comprising polynucleotides which encode the amino acid sequences of epitope-bearing portions of a E. faecalis polypeptide having an amino acid sequence in above.

The present invention also relates to recombinant vectors, which include the isolated nucleic acid molecules of the present invention, and to host cells containing the recombinant vectors, as well as to methods of making such vectors and host cells.

The present invention further relates to the use of these vectors in the production of E. faecalis polypeptides or peptides by recombinant techniques.

The invention further provides isolated E. faecalis polypeptides having an amino acid sequence selected from the group consisting of an amino acid sequence of any of the polypeptides described in Table 1 or fragments thereof.

The polypeptides of the present invention also include polypeptides having an amino acid sequence with at least 70% similarity, and more preferably at least 85%, 90%, 95%, 96%, 97%, 98%, or 99% similarity to those described in Table 1, as well as polypeptides having an amino acid sequence at least 70% identical, more preferably at least 75% identical, and still more preferably 80%, 85%, 90%, 96%, 97%, 98%, or 99% identical to those above; as well as isolated nucleic acid molecules encoding such polypeptides.

The present invention further provides a single or multi-component vaccine comprising one or more of the E. faecalis polynucleotides or polypeptides described in Table 1, or fragments thereof, together with a pharmaceutically acceptable diluent, carrier, or excipient, wherein the E. faecalis polypeptide(s) are present in an amount effective to elicit an immune response to members of the Enterococcus genus, or at least E. faecalis in an animal. The E. faecalis polypeptides of the present invention may further be combined with one or more immunogens of one or more other Enterococcal or non-Enterococcal organisms to produce a multi-component vaccine intended to elicit an immunological response against members of the Enterococcus genus and, optionally, one or more non-Enterococcal organisms.

The vaccines of the present invention can be administered in a DNA form, e.g., "naked" DNA, wherein the DNA encodes one or more Enterococcal polypeptides and, optionally, one or more polypeptides of a non-Enterococcal organism. The DNA encoding one or more polypeptides may be constructed such that these polypeptides are expressed as fusion proteins.

The vaccines of the present invention may also be administered as a component of a genetically engineered organism or host cell. Thus, a genetically engineered organism or host cell which expresses one or more E. faecalis polypeptides may be administered to an animal. For example, such a genetically engineered organism or host cell may contain one or more E. faecalis polypeptides of the present invention intracellularly, on its cell surface, or in its periplasmic space. Further, such a genetically engineered organism or host cell may secrete one or more E. faecalis polypeptides. The vaccines of the present invention may also be co-administered to an animal with an immune system modulator CD86 and GM-CSF).

o The invention also provides a method of inducing an immunological response in an animal to one or more members of the Enterococcus genus, preferably one or 15 more isolates of the E.faecalis species, comprising administering to the animal a vaccine as described above.

The invention further provides a method of inducing a protective immune response in an animal, sufficient to prevent, attenuate, or control an infection by members of the Enterococcus genus, preferably at least E. faecalis species, comprising administering to the animal a composition comprising one or more of the polynucleotides or polypeptides described in Table 1, or fragments thereof. Further, these polypeptides, or fragments thereof, may be conjugated to another immunogen and/or administered in admixture with an adjuvant.

The invention further relates to antibodies elicited in an animal by the administration of one or more E. faecalis polypeptides of the present invention and to methods for producing such antibodies and fragments thereof. The invention further relates to recombinant antibodies and fragments thereof and to methods for producing such antibodies and fragments thereof.

The invention also provides diagnostic methods for detecting the expression of the polynucleotides of Table 1 by members of the Enterococcus genus in an animal.

One such method involves assaying for the expression of a polynucleotide encoding E. faecalis polypeptides in a sample from an animal. This expression may be assayed either directly by assaying polypeptide levels using antibodies elicited in response to amino acid sequences described in Table 1) or indirectly by assaying for antibodies having specificity for amino acid sequences described in Table The expression of polynucleotides can also be assayed by detecting the nucleic acids of Table 1. An example of such a method involves the use of the polymerase chain reaction (PCR) to amplify and detect Enterococcus nucleic acid sequences.

The present invention also relates to nucleic acid probes having all or part of a nucleotide sequence described in Table 1 (odd SEQ ID NOs) which are capable of hybridizing under stringent conditions to Enterococcus nucleic acids. The invention further relates to a method of detecting one or more Enterococcus nucleic acids in a biological sample obtained from an animal, said one or more nucleic acids encoding 15 Enterococcus polypeptides, comprising: contacting the sample with one or more of the above-described nucleic acid probes, under conditions such that hybridization occurs, and detecting hybridization of said one or more probes to the Enterococcus nucleic acid present in the biological sample.

Other uses of the polypeptides of the present invention include: inter alia. to 20 detect E. faecalls in immunoassays, as epitope tags, as molecular weight markers on SDS-PAGE gels, as molecular weight markers for molecular sieve gel filtration columns, to generate antibodies that specificaly bind E. faecalis polypeotides of the present invention for the detection E. faecalis in immunoassays, to generate an immune response against E. faecalis and other Enterococcus species, and as vaccines against E. faecalis, other Enterococcus species and other bacteria genuses.

Isolated nucleic acid molecules of the present invention, particularly DNA molecules, are useful as probes for gene mapping and for identifying E. faecalis in a biological samples, for instance, by Southern and Northern blot analysis.

Polynucleotides of the present invention are also useful in detecting E. faecalis by PCR using primers for a particular E. faecalis polynucleotide. Isolated polynucleotides of the present invention are also useful in making the polypeptides of the present invention.

Detailed Description The present invention relates to recombinant E. faecalis nucleic acids and fragments thereof. The present invention further relates to recombinant E. faecalis polypeptides and fragments thereof. The invention also relates to methods for using these polypeptides to produce immunological responses and to confer immunological In protection to disease caused by members of the genus Enterococcus, at least isolates of the E. faecalis genus. The invention further relates to nucleic acid sequences which encode antigenic E. faecalis polypeptides and to methods for detecting E. faecalis nucleic acids and polypeptides in biological samples. The invention also relates to antibodies specific for the polypeptides and peptides of the present invention and 15 methods for detecting such antibodies produced in a host animal.

S* Definitions The following definitions are provided to clarify the subject matter which the inventors consider to be the present invention.

20 As used herein, the phrase "pathogenic agent" means an agent which causes a disease state or affliction in an animal. Included within this definition, for examples, are bacteria, protozoans, fungi, viruses and metazoan parasites which either produce a disease state or render an animal infected with such an organism susceptible to a disease state a secondary infection). Further included are species and strains of the genus Enterococcus which produce disease states in animals.

As used herein, the term "organism" means any living biological system, including viruses, regardless of whether it is a pathogenic agent.

As used herein, the term "Enterococcus" means any species or strain of bacteria which is members of the genus Enterococcus. Such species and strains are known to those of skill in the art, and include those that are pathogenic and those that are not.

As used herein, the phrase "one or more E. faecalis polypeptides of the present invention" means polypeptides comprising the amino acid sequence of one or more of the E. faecalis polypeptides described in Table 1 (even SEQ ID NOs). These polypeptides may be expressed as fusion proteins wherein the E. faecalis polypeptides of the present invention are linked to additional amino acid sequences which may be of Enterococcal or non-Enterococcal origin. This phrase further includes polypeptide comprising fragments of the E. faecalis polypeptides of the I present invention. Additional definitions are provided throughout the specification.

Explanation of Table I Table 1, below, provides information describing genes which encode polypeptides of E.faecalis. The table lists the gene identifier which consists of the 15 letters EF, which denote E.faecalis, followed imunediately by a three digit numeric code, which arbitrarily number the E.faecalis genes of the present invention. A number from 1 through 4 follows the three digit number. A number 1 represents the full length open reading frame of the gene specified by the preceeding three digit number. A number 2 represents the full leng-tpolypeptide encoded by the gene specified the preceeding three digit number. A number 3 represents a polynucleotide fragment, of the gene represented by the preceeding three digit number, used to produce an antigenic polypeptide. A number 4 represents an antigenic polypeptide fragment, of the gene represented by the preceeding three digit number, used to stimulate an immune response or as a vaccine. The nucleotide and amino acid sequences of each gene and fragment are also shown in the Sequence Listing under the SEQ ID NO listed in Table 1.

Explanation of Table 2 Table 2 lists accession numbers for the closest matching sequences between the polypeptides of the present invention and those available through GenBank and Derwent databases. These reference numbers are the database entry numbers commonly used by those of skill in the art, who will be familar with their denominations. The descriptions of the numenclature for GenBank are available from the National Center for Biotechnology Information. Column I lists the gene or ORF of the present invention. Column 2 lists the accession number of a "match" gene sequence in GenBank or Derwent databases. Column 3 lists the description of the "match" gene sequence. Columns 4 and 5 are the high score and smallest sum probability, respectively, calculated by BLAST. Polypeptides of the present invention that do not share significant identity/similarity with any polypeptide sequences of GenBank and Derwent are not represented in Table 2. Polypeptides of the present invention that share significant identity/similarity with more than one of the polypeptides of GenBank and Derwent are represented more than once.

15 Explanation of Table 3.

The E. faecalis polypeptides of the present invention may include one or more conservative amino acid substitutions from natural mutations or human manipulation as indicated in Table 3. Changes are preferably of a minor nature, such as conservative amino acid substitutions that do not significantly affect the folding or activity of the 20 protein. Residues from the following groups, as indicated in Table 3, may be substituted for one another: Aromatic, Hydrophobic, Polar, Basic, Acidic, and Small, Explanation of Table 4 Table 4 lists residues comprising antigenic epitopes of antigenic epitopebearing fragments present in each of the full length E. faecalis polypeptides described in Table 1 as predicted by the inventors using the algorithm of Jameson and Wolf, (1988) Comp. Appl. Biosci. 4:181-186. The Jameson-Wolf antigenic analysis was performed using the computer program PROTEAN (Version 3.11 for the Power Macintosh, DNASTAR, Inc., 1228 South Park Street Madison, WI). E. faecalis polypeptide shown in Table 1 may one or more antigenic epitopes comprising residues described in Table 4. It will be appreciated that depending on the analytical criteria used to predict antigenic determinants, the exact address of the determinant may vary slightly. The residues and locations shown described in Table 4 correspond to the amino acid sequences for each full length gene sequence shown in Table I and in the Sequence Listing. Polypeptides of the present invention that do not have antigenic epitopes recognized by the Jameson-Wolf algorithm are not represented in Table 2.

Selection of Nucleic Acid Sequences Encoding Antigenic E. faecalis Polypeptides Sequenced E. faecalis genomic DNA was obtained from the E. faecalis strain V586. The E. faecalis strain V586 was deposited 2 May 1997 at the ATCC, 10801 e" University Blvd. Manassas, VA 20110-2209, and given accession number 55969.

Some ORFs contained in the subset of fragments of the E. faecalis genome 15 disclosed herein were derived through the use of a number of screening criteria detailed below. The ORFs are bounded at the amino terminus by a methionine or valine residue and usually at the carboxy terminus by a stop codon.

Most of the selected sequences consist of complete ORFs. The polypeptides that do not comprise a complete ORF can be determined by determining whether the 20 corresponding polynucleotide sequence comprises a stop codon after the codon for the last amino acid residue in the polypeptide sequence. It is not always preferred to a express a complete ORF in a heterologous system. It may be challenging to express and purify a highly hydrophobic protein by common laboratory methods. Some of the polypeptide vaccine candidates described herein have been modified slightly to simplify the production of recombinant protein. For example, nucleotide sequences which encode highly hydrophobic domains, such as those found at the amino terminal signal sequence, have been excluded from some constructs used for expression of the polypeptides. Furthermore, any highly hydrophobic amino acid sequences occurring at the carboxy terminus have also been excluded from the recombinant expression constructs. Thus, in one embodiment, a polypeptide which represents a truncated or modified ORF may be used as an antigen.

While numerous methods are known in the art for selecting potentially immunogenic polypeptides, many of the ORFs disclosed herein were selected on the basis of screening Enterococcusfaecalis ORFs for several aspects of potential irmnunogenicity. One set of selection criteria are as follows: 1. Type I signal sequence: An amino terminal type I signal sequence generally directs a nascent protein across the plasma and outer membranes to the exterior of the bacterial cell. Experimental evidence obtained from studies with Escherichia coli suggests that the typical type I signal sequence consists of the following biochemical and physical attributes (Izard, J. W. and Kendall, D. A. Mol. Microbiol. 13:765-773 (1994)). The length of the type I signal sequence is approximately 15 to 25 primarily hydrophobic amino acid residues with a net positive charge in the extreme amino terminus. In addition, the central region of the signal sequence. adopts an alpha-helical conformation in a hydrophobic environment. Finally, the region surrounding the actual site of cleavage is ideally six residues long, with small side-chain amino acids in i the -1 and -3 positions.

2. Type IV signal sequence: The type IV signal sequence is an example of the several types of functional signal sequences which exist in addition to the type I signal 20 sequence detailed above. Although functionally related, the type IV signal sequence possesses a unique set of biochemical and physical attributes (Strom, M. S. and Lory, J. Bacteriol. 174:7345-7351 (1992)). These are typically six to eight amino acids with a net basic charge followed by an additional sixteen to thirty primarily hydrophobic residues. The cleavage site of a type IV signal sequence is typically after the initial six to eight amino acids at the extreme amino terminus. In addition, type IV signal sequences generally contain a phenylalanine residue at the +1 site relative to the cleavage site.

3. Lipoprotein: Studies of the cleavage sites of twenty-six bacterial lipoprotein precursors has allowed the definition of a consensus amino acid sequence for lipoprotein cleavage. Nearly three-fourths of the bacterial lipoprotein precursors examined contained the sequence at positions -3 to relative to the point of cleavage (Hayashi, S. and Wu, H. Bioenerg. Biomembr. 22:451-471 (1990)).

4. LPXTG motif It has been experimentally determined that most anchored proteins found on the surface of gram-positive bacteria possess a highly conserved carboxy terminal sequence. More than fifty such proteins from organisms such as S.

pyogenes, S. mutans, E. faecalis, S. pneumoniae, and others, have been identified based on their extracellular location and carboxy terminal amino acid sequence (Fischetti, V.

ASMNews 62:405-410 (1996)). The conserved region consists of six charged amino acids at the extreme carboxy terminus coupled to 15-20 hydrophobic amino acids presumed to function as a transmembrane domain. Immediately adjacent to the transmembrane domain is a six amino acid sequence conserved in nearly all proteins examined. The amino acid sequence of this region is L-P-X-T-G-X, where X is any amino acid.

An algorithm for selecting antigenic and immunogenic Enterococcusfaecalis "mo: polypeptides including the foregoing criteria was developed. The algorithm is similar to that described in U.S. patent application 08/781,986, filed January 3, 1997, which is fully incorporated by reference herein. Use of the algorithm by the inventors to select immunologically useful Enterococcusfaecalis polypeptides resulted in the selection of a number of the disclosed ORFs. Polypeptides comprising the polypeptides identified in this group may be produced by techniques standard in the art and as further described herein.

Nucleic Acid Molecules Sequenced E.faecalis genomic DNA was obtained from the E. faecalis strainV586. As discussed elsewhere hererin, polynucleotides of the present invention readily may be obtained by routine application of well known and standard procedures for cloning and sequencing DNA. Detailed methods for obtaining libraries and for sequencing are provided below, for instance. A wide variety of Enterococcusfaecalis strains that can be used to prepare E.faecalis genomic DNA for cloning and for obtaining polynucleotides and polypeptides of the present invention. A wide variety of Enterococcusfaecalis strains are available to the public from recognized depository institutions, such as the American Type Culture Collection (ATCC). It is recognized that minor variation is the nucleic acid and amino acid sequence may be expected from Efaecalis strain to strain. The present invention provides for genes, including both polynucleotides and polypeptides, of the of the present invention from all the Enterococcusfaecalis strains.

Unless otherwise indicated, all nucleotide sequences determined by sequencing a DNA molecule herein were determined using an automated DNA sequencer (such as the Model 373 from Applied Biosystems, Inc., Foster City, CA), and all amino acid sequences of polypeptides encoded by DNA molecules determined herein were predicted by translation of a DNA sequence determined as above. Therefore, as is 15 known in the art for any DNA sequence determined by this automated approach, any nucleotide sequence determined herein may contain some errors. Nucleotide sequences detennined by automation are typically at least about 90% identical, more typically at least about 95% to at least about 99.9% identical to the actual nucleotide sequence of the sequenced DNA molecule. The actual sequence can be more precisely determined by other approaches including manual DNA sequencing methods well known in the art. As is also known in the art, a single insertion or deletion in a determined nucleotide sequence compared to the actual sequence will cause a frame shift in translation of the nucleotide sequence such that the predicted amino acid sequence encoded by a determined nucleotide sequence will be completely different from the amino acid sequence actually encoded by the sequenced DNA molecule, beginning at the point of such an insertion or deletion. In case of conflict between Table 1 and either the nucleic acid sequence of the clones listed in Table 1 or the amino acid sequence of the protein expressed by the clones listed in Table 1, the clones listed in Table 1 are controlling. By "nucleotide sequence" of a nucleic acid molecule or polynucleotide is intended to mean either a DNA or RNA sequence.Using the information provided herein, such as the nucleotide sequence in Table 1, a nucleic acid molecule of the present invention encoding a E. faecalis polypeptide may be obtained using standard cloning and screening procedures, such as those for cloning DNAs using genomic DNA as starting material. See, Sambrook et al. MOLECULAR CLONING: A LABORATORY MANUAL (Cold Spring Harbor, N.Y. 2nd ed.

1989); Ausubel et al., CURRENT PROTOCALS IN MOLECULAR BIOLOGY (John Wiley and Sons, N.Y. 1989). Illust ative of the invention, the nucleic acid molecule described in Table 1 was discovered in a DNA library derived from a E.

faecalis genomic DNA.

Nucleic acid molecules of the present invention may be in the form of RNA, such as mRNA, or in the form of DNA, including, for instance, DNA and genomic DNA obtained by cloning or produced synthetically. The DNA may be double-stranded or single-stranded. Single-stranded DNA or RNA may be the coding 15 strand, also known as the sense strand, or it may be the non-coding strand, also referred to as the anti-sense strand.

By "isolated" nucleic acid molecule(s) is intended a nucleic acid molecule, DNA or RNA, which has been removed from its native environment. This includes segments of DNA comprising the E. faecalis polynucleotides of the present invention isolated from the native chromosome. These fragments include both isolated fragments consisting only of E. faecalis DNA and fragments comprising heterologous sequences such as vector sequences or other foreign DNA. For example, recombinant DNA molecules contained in a vector are considered isolated for the purposes of the present invention. Further examples of isolated DNA molecules include recombinant DNA molecules maintained in heterologous host cells or purified (partially or substantially) DNA molecules in solution. Isolated RNA molecules include in vivo or in vitro RNA transcripts of the DNA molecules of the present invention. Isolated nucleic acid molecules according to the present invention further include such molecules produced synthetically.

In addition, isolated nucleic acid molecules of the invention include DNA molecules which comprise a sequence substantially different from those described above but which, due to the degeneracy of the genetic code, still encode a E. faecalis polypeptides and peptides of the present invention polypeptides of Table 1).

That is, all possible DNA sequences that encode the E. faecalis polypeptides of the present invention. This includes the genetic code and species-specific codon preferences known in the art. Thus, it would be routine for one skilled in the art to generate the degenerate variants described above, for instance, to optimize codon expression for a particular host change codons in the bacteria mRNA to those preferred by a mammalian or other bacterial host such as E. coli).

The invention further provides isolated nucleic acid molecules having the nucleotide sequence shown in Table 1 or a nucleic acid molecule having a sequence complementary to one of the above sequences. Such isolated molecules, particularly DNA molecules, are useful as probes for gene mapping and for identifying E.faecalis in a biological sample, for instance, by PCR, Southern blot, Northern blot, or other form of hybridization analysis.

The present invention is further directed to nucleic acid molecules encoding portions or fragments of the nucleotide sequences described herein. Fragments include S* portions of the nucleotide sequences of Table 1, or the E. faecalis nucleotide sequences contained in the plasimd clones listed in Table 1, at least 10 contiguous nucleotides in length selected from any two integers, one of which representing a nucleotide position and a second of which representing a 3' nucleotide position, where the first nucleotide for each nucleotide sequence in Table 1 is position 1. That is, every combination of a 5' and 3' nucleotide position that a fragment at least contiguous nucleotides in length could occupy is included in the invention. At least means a fragment may be 10 contiguous nucleotide bases in length or any integer between 10 and the length of an entire nucleotide sequence of Table 1 minus 1.

Therefore, included in the invention are contiguous fragments specified by any 5' and 3' nucleotide base positions of a nucleotide sequences of Table 1 wherein the contiguous fragment is any integer between 10 and the length of an entire nucleotide sequence minus 1.

Further, the invention includes polynucleotides comprising fragments specified by size, in nucleotides, rather than by nucleotide positions. The invention includes any fragment size, in contiguous nucleotides, selected from integers between 10 and the length of an entire nucleotide sequence minus 1. Preferred sizes of contiguous nucleotide fragments include 20 nucleotides, 30 nucleotides, 40 nucleotides, nucleotides. Other preferred sizes of contiguous nucleotide fragments, which may be useful as diagnostic probes and primers, include fragments 50-300 nucleotides in length which include, as discussed above, fragment sizes representing each integer between 50-300. Larger fragments are also useful according to the present invention corresponding to most, if not all, of the nucleotide sequences shown in Table 1 or of the E. faecalis nucleotide sequences of the plasimd clones listed in Table 1. The preferred sizes are, of course, meant to exemplify not limit the present invention as all size fragments, representing any integer between 10 and the length of an entire Snucleotide sequence minus 1, are included in the invention. Additional preferred nucleic acid fragments of the present invention include nucleic acid molecules encoding epitope-bearing portions of E. faecalis polypeptides identified in Table 4.

•The present invention also provides for the exclusion of any fragment, specified by 5' and 3' base positions or by size in nucleotide bases as described above for any nucleotide sequence of Table 1 or the plasimd clones listed in Table 1. Any number of fragments of nucleotide sequences in Table 1 or the plasimd clones listed in Table 1, specified by 5' and 3' base positions or by size in nucleotides, as described above, may be excluded from the present invention.

In another aspect, the invention provides an isolated nucleic acid molecule comprising a polynucleotide which hybridizes under stringent hybridization conditions to a portion of a polynucleotide in a nucleic acid molecules of the invention described above, for instance, nucleotide sequences of Table 1 or the E. faecalis sequences of the plasimd clones listed in Table 1. By "stringent hybridization conditions" is intended overnight incubation at 42 0 C in a solution comprising: formamide, 5x SSC (150 mM NaCI, 15 mM trisodium citrate), 50 mM sodium phosphate (pH 5x Denhardt's solution, 10% dextran sulfate, and 20 p.g/ml denatured, sheared salmon sperm DNA, followed by washing the filters in 0. x SSC at about By a polynucleotide which hybridizes to a "portion" of a polynucleotide is intended a polynucleotide (either DNA or RNA) hybridizing to at least about nucleotides bases, and more preferably ai least about 20 nucleotides bases, still more preferably at least about 30 nucleotides bases, and even more preferably about 30-70 50) nucleotides bases of the reference polynucleotide. These are useful as diagnostic probes and primers as discussed above. By a portion of a polynucleotide of "at least 20 nucleotides bases in length," for example, is intended 20 or more contiguous nucleotides bases nucleotides from the nucleotide sequence of the reference "polynucleotide the nucleotide sequence as shown in Table Portions of a polynucleotide which hybridizes to a nucleotide sequence in Table 1, which can be used as probes and primers, may also be precisely specified by 5' and 3' base positions or by size in nucleotide bases as described above or precisely excluded in the same manner.

The nucleic acid molecules of the present invention include those encoding the full length E.faecalis polypeptides of Table 1 and portions of the E. faecalis polypeptides of Table 1. Also included in the present invention are nucleic acids encoding the above full length sequences and further comprise additional sequences, such as those encoding an added secretory leader sequence, such as a pre-, or pro- or prepro- protein sequence. Further included in the present invention are nucleic acids encoding the above full length sequences and portions thereof and further comprise additional heterologous amino acid sequences encoded by nucleic acid sequences from a different source.

Also included in the present invention are nucleic acids encoding the above protein sequences together with additional, non-coding sequences, including for example, but not limited to non-coding 5' and 3' sequences. These sequences include transcribed, non-translated sequences that may play a role in transcription, and mRNA processing, for example, ribosome binding and stability ofmRNA. Also included in the present invention are additional coding sequences which provide additional functionalities.

Thus, a nucleotide sequence encoding a polypeptide may be fused to a marker sequence, such as a sequence encoding a peptide which facilitates purification of the fused polypeptide. In certain preferred 6imbodiments of this aspect of the invention, the marker amino acid sequence is a hexa-histidine peptidd, such as the tag provided in a pQE vector (QIAGEN, Inc., 9259 Eton Avenue, Chatsworth, CA, 91311), among others, many of which are commercially available. For instance, hexa-histidine provides for convenient purification of the fusion protein. See Gentz et al. (1989) Proc. Natl. Acad. Sci. 86:821-24. The "HA" tag is another peptide useful for purification which corresponds to an epitope derived from the influenza hemagglutinin protein. See Wilson et al. (1984) Cell 37:767. As discussed below, other such fusion proteins include the E. faecalis polypeptides of the present invention fused to Fc at the N- or C-terminus.

Variant and Mutant Polynucleotides The present invention further relates to variants of the nucleic acid molecules which encode portions, analogs or derivatives of a E. faecalis polypeptides of Table 1 S[and variant polypeptides thereof including portions, analogs, and derivatives of the E.

faecalis polypeptides. Variants may occur naturally, such as a natural allelic variant.

By an "allelic variant" is intended one of several alternate forms of a gene occupying a given locus on a chromosome of an organism. See, B. Lewin, Genes IV (1990).

Non-naturally occurring variants may be produced using art-known mutagenesis techniques.

Such nucleic acid variants include those produced by nucleotide substitutions, deletions, or additions. The substitutions, deletions, or additions may involve one or more nucleotides. The variants may be altered in coding regions, non-coding regions, or both. Alterations in the coding regions may produce conservative or non-conservative amino acid substitutions, deletions or additions. Especially preferred among these are silent substitutions, additions and deletions, which do not alter the properties and activities of a E. faecalis protein of the present invention or portions thereof. Also especially preferred in this regard are conservative substitutions.

Such polypeptide variants include those produced by amino acid substitutions, deletions or additions. The substitutions, deletions, or additions may involve one or more residues. Alterations may produce conservative or non-conservative amino acid substitutions, deletions, or additions. Especially preferred among these are silent substitutions, additions and deletions, which do not alter the properties and activities of a E. faecalis protein of the present invention or "portions thereof. Also especially preferred in this regard are conservative substitutions.

SThe present invention also relates to recombinant vectors, which include the S. isolated nucleic acid molecules of the present invention, and to host cells containing the recombinant vectors, as well as to methods of making such vectors and host cells S"and for using them for production of E. faecalis polypeptides or peptides by recombinant techniques.

The present application is directed to nucleic acid molecules at least 95%, 96%, 97%, 98% or 99% identical to a nucleic acid sequence shown in Table 1.

The above nucleic acid sequences are included irrespective of whether they encode a polypeptide having E. faecalis activity. This is because even where a particular nucleic acid molecule does not encode a polypeptide having E. faecalis activity, one of skill in the art would still know how to use the nucleic acid molecule, for instance, as a hybridization probe. Uses of the nucleic acid molecules of the present invention that do not encode a polypeptide having E. faecalis activity include, inter alia, isolating an E. faecalis gene or allelic variants thereof from a DNA library, and detecting E. faecalis mRNA expression samples, environmental samples, suspected of containing E.

faecalis by Northern Blot analysis.

Preferred, are nucleic acid molecules having sequences at least 90%, 95%, 96%, 97%, 98% or 99% identical to the nucleic acid sequence shown in Table 1, which do, in fact, encode a polypeptide having E. faecalis protein activity By "a polypeptide having E. faecalis activity" is intended polypeptides exhibiting activity similar, but not necessarily identical, to an activity of the E. faecalis protein of the invention, as measured in a particular biological assay suitable for measuring activity of the specified protein.

Due to the degeneracy of the genetic code, one of ordinary skill in the art will immediately recognize that a large number of the nucleic acid molecules having a sequence at least 90%, 95%, 96%, 97%, 98%, or 99% identical to the nucleic acid sequences shown in Table I will encode a polypeptide having E. faecalis protein activity. In fact, since degenerate variants of these nucleotide sequences all encode the same polypeptide, this will be clear to the skilled artisan even without performing the above described comparison assay. It will be further recognized in the art that, for such nucleic acid molecules that are not degenerate variants, a reasonable number will also encode a polypeptide having E. faecalis protein activity. This is because the skilled artisan is fully aware of amino acid substitutions that are either less likely or not likely to significantly effect protein function replacing one aliphatic amino acid with a second aliphatic amino acid), as further described below.

The biological activity or function of the polypeptides of the present invention are expected to be similar or identical to polypeptides from other bacteria that share a high degree of structural identity/similarity. Tables 2 lists accession numbers and descriptions for the closest matching sequences of polypeptides available through Genbank and Derwent databases. It is therefore expected that the biological activity or function of the polypeptides of the present invention will be similar or identical to those polypeptides from other bacterial genuses, species, or strains listed in Table 2.

By a polynucleotide having a nucleotide sequence at least, for example, "identical" to a reference nucleotide sequence of the present invention, it is intended that the nucleotide sequence of the polynucleotide is identical to the reference sequence except that the polynucleotide sequence may include up to five point s mutations per each 100 nucleotides of the reference nucleotide sequence encoding the E. faecalis polypeptide. In other words, to obtain a polynucleotide having a nucleotide sequence at least 95% identical to a reference nucleotide sequence, up to of the nucleotides in the reference sequence may be deleted, inserted, or substituted with another nucleotide. The query sequenceanay be an entire sequence n1 shown in Table 1, the ORF (open reading frame), or any fragment specified as described herein.

As a practical matter, whether any particular nucleic acid molecule or polypeptide is at least 90%, 95%, 96%, 97%, 98% or 99% identical to a nucleotide S sequence of the presence invention can be determined conventionally using known computer programs. A preferred method for determining the best overall match between a query sequence (a sequence of the present invention) and a subject sequence, also referred to as a global sequence alignment, can be determined using the FASTDB computer program based on the algorithm of Brutlag et al. See Brutlag et al. (1990) Comp. App. Biosci. 6:237-245. In a sequence alignment the query and subject sequences are both DNA sequences. An RNA sequence can be compared by first converting U's to T's. The result of said global sequence alignment is in percent identity. Preferred parameters used in a FASTDB alignment of DNA sequences to calculate percent identity are: Matrix=Unitary, k-tuple=4, Mismatch Penalty 1, Joining Penalty=30, Randomization Group Length=0, Cutoff Score=l, Gap *4 Penalty=5, Gap Size Penalty 0.05, Window Size=500 or the lenght of the subject nucleotide sequence, whichever is shorter.

If the subject sequence is shorter than the query sequence because of 5' or 3' deletions, not because of internal deletions, a manual correction must be made to the results. This is because the FASTDB program does not account for 5' and 3' truncations of the subject sequence when calculating percent identity. For subject sequences truncated at the 5' or 3' ends, relative to the query sequence, the percent identity is corrected by calculating the number of bases of the query sequence that are and 3' of the subject sequence, which are not matched/aligned, as a percent of the total bases of the query sequence. Whether a nucleotide is matched/aligned is determined by results of the FASTDB sequence alignment. This percentage is then subtracted from the percent identity, calculated by the above FASTDB program using the specified parameters, to arrive at a final percent identity score. This corrected score is what is used for the purposes of the present invention. Only nucleotides outside the 5' and 3' nucleotides of the subject sequence, as displayed by the FASTDB alignment, which are not matched/aligned with the query sequence, are calculated for the purposes of manually adjusting the percent identity score.

For example, a 90 nucleotide subject sequence is aligned to a 100 nucleotide query sequence to determine percent identity. The deletions occur at the 5' end of the 15 subject sequence and therefore, the FASTDB alignment does not show a matched/alignment of the first 10 nucleotides at 5' end. The 10 unpaired nucleotides represent 10% of the sequence (number of nucleotides at the 5' and 3' ends not matched/total number of nucleotides in the query sequence) so 10% is subtracted from the percent identity score calculated by the FASTDB program. If the remaining 20 nucleotides were perfectly matched the final percent identity would be 90%. In another example, a 90 nucleotide subject sequence is compared with a 100 nucleotide query sequence. This time the deletions are internal deletions so that there are no nucleotides on the 5' or 3' of the subject sequence which are not matched/aligned with the query. In this case the percent identity calculated by FASTDB is not manually corrected. Once again, only nucleotides 5' and 3' of the subject sequence which are not matched/aligned with the query sequence are manually corrected for. No other manual corrections are to made for the purposes of the present invention.

Vectors and Host Cell The present invention also relates to vectors which include the isolated DNA molecules of the present invention, host cells comprising the recombinant vectors, and the production of E. faecalis polypeptides and peptides of the present invention expressed by the host cells.

Recombinant constructs may be introduced into host cells using well known techniques such as infection, transduction, transfection, transvection, electroporation and transformation. The vector may be, for example, a phage, plasmid, viral or retroviral vector. Retroviral vectors may be replication competent or replication defective. In the latter case, viral propagation generally Will occur only in complementing host cells.

The polynucleotides may be joined to a vector containing a selectable marker for propagation in a host. Generally, a plasmid vector is introduced in a precipitate, such as a calcium phosphate precipitate, or in a complex with a charged lipid. If the vector is a virus, it may be packaged in vitro using an appropriate packaging cell line 15 and then transduced into host cells.

Preferred are vectors comprising cis-acting control regions to the S. polynucleotide of interest. Appropriate trans-acting factors may be supplied by the host, supplied by a complementing vector or supplied by the vector itself upon introduction into the host.

In certain preferred embodiments in this regard, the vectors provide for specific expression, which may be inducible and/or cell type-specific. Particularly preferred among such vectors are those inducible by environmental factors that are easy to manipulate, such as temperature and nutrient additives.

Expression vectors useful in the present invention include chromosomal-, episomal- and virus-derived vectors, vectors derived from bacterial plasmids, bacteriophage, yeast episomes, yeast chromosomal elements, viruses such as baculoviruses, papova viruses, vaccinia viruses, adenoviruses, fowl pox viruses, pseudorabies viruses and retroviruses, and vectors derived from combinations thereof, such as cosmids and phagemids.

The DNA insert should be operatively linked to an appropriate promoter, such as the phage lambda PL promoter, the E. coli lac, trp and lac promoters, the early and late promoters and promoters of retroviral LTRs, to name a few.

Other suitable promoters will be known to the skilled artisan. The expression constructs will further contain sites for transcription initiation, termination and, in the transcribed region, a ribosome binding site for translation. The coding portion of the mature transcripts expressed by the constructs will preferably include a translation initiating site at the beginning and a termination codon (UAA, UGA or UAG) appropriately positioned at the end of the polypeptide to.be translated.

As indicated, the expression vectors will preferably include at least one selectable marker. Such markers include dihydrofolate reductase or neomycin resistance for eukaryotic cell culture and tetracycline, kanamycin, or ampicillin resistance genes for culturing in E. coli and other bacteria. Representative examples of appropriate hosts include, but are not limited to, bacterial cells, such as E. coli, Streptomyces and Salmonella typhimurium cells; fungal cells, such as yeast cells; insect cells such as Drosophila S2 and Spodoptera Sf9 cells; animal cells such as CHO, COS and Bowes melanoma cells; and plant cells. Appropriate culture mediums and conditions for the above-described host cells are known in the art.

Among vectors preferred for use in bacteria include pQE70, pQE60 and pQE9, pQE10 available from Qiagen; pBS vectors, Phagescript vectors, Bluescript vectors, pNH8A, pNH 16a, pNH18A, pNH46A available from Stratagene; pET series of vectors available from Novagen; and ptrc99a, pKK223-3, pKK233-3, pDR540, available from Pharmacia. Among preferred eukaryotic vectors are pWLNEO, pSV2CAT, pOG44, pXTI and pSG available from Stratagene; and pSVK3, pBPV, pMSG and pSVL available from Pharmacia. Other suitable vectors will be readily apparent to the skilled artisan.

Among known bacterial promoters suitable for use in the present invention include the E. coli lad and lacZ promoters, the T3, T5 and T7 promoters, the gpt promoter, the lambda PR and PL promoters and the trp promoter. Suitable eukaryotic promoters include the CMV immediate early promoter, the HSV thymidine kinase promoter, the early and late SV40 promoters, the promoters of retroviral LTRs, such as those of the Rous sarcoma virus (RSV), and metallothionein promoters, such as the mouse metallothioiein-1 promoter.

Introduction of the construct into the host cell can be effected by calcium phosphate transfection, DEAE-dextran mediated transfection, cationic lipid-mediated transfection, electroporation, transduction, infection or other methods. Such methods are described in many standard laboratory manuals (for example, Davis, et al., Basic Methods In Molecular Biology (1986)).

Transcription of DNA encoding the polypeptides of the present invention by higher eukaryotes may be increased by inserting an enhancer sequence into the vector.

Enhancers are cis-acting elements of DNA, usually about from 10 to 300 nucleotides that act to increase transcriptional activity of a promoter in a given host cell-type.

Examples of enhancers include the SV40 enhancer, which is located on the late side of 15 the replication origin at nucleotides 100 to 270, the cytomegalovirus early promoter enhancer, the polyoma enhancer on the late side of the replication origin, and adenovirus enhancers.

For secretion of the translated polypeptide into the lumen of the endoplasmic reticulum, into the periplasmic space or into the extracellular environment, 20 appropriate secretion signals may be incorporated into the expressed polypeptide, for example, the amino acid sequence KDEL. The signals may be endogenous to the polypeptide or they may be heterologous signals.

The polypeptide may be expressed in a modified form, such as a fusion protein, and may include not only secretion signals, but also additional heterologous functional regions. For instance, a region of additional amino acids, particularly charged amino acids, may be added to the N-terminus of the polypeptide to improve stability and persistence in the host cell, during purification, or during subsequent handling and storage. Also, peptide moieties may be added to the polypeptide to facilitate purification. Such regions may be removed prior to final preparation of the polypeptide. The addition of peptide moieties to polypeptides to engender secretion or excretion, to improve stability and to facilitate purification, among others, are familiar and routine techniques in the art. A preferred fusion protein comprises a heterologous region from immunoglobulin that is useful to solubilize proteins. For example, EP-A-O 464 533 (Canadian counterpart 2045869) discloses fusion proteins comprising various portions of constant region of immunoglobulin molecules together with another human protein or part thereof. In many cases, the Fc part in a fusion protein is thoroughly advantageous for use in therapy and diagnosis and thus results, for example, in improved pharmacokinetic properties (EP-A 0232 262). On the other hand, for some uses it would be desirable to be able to delete the Fc part after the fusion protein has been expressed, detected and purified in the advantageous manner described. This is the case when Fc portion proves to be a hindrance to use in therapy and diagnosis, for example when the fusion protein is to be used as antigen for immunizations. In drug discovery, for example, human proteins, such as, hlL5-receptor has been fused with Fc portions for the purpose of high-throughput screening assays to identify antagonists of hIL-5. See Bennett, D. et al. (1995) J.

Molec. Recogn. 8:52-58 and Johanson, K. et al. (1995) J. Biol. Chem. 270 (16):9459-9471.

The E. faecalis polypeptides can be recovered and purified from recombinant 20 cell cultures by well-known methods including ammonium sulfate or ethanol precipitation, acid extraction, anion or cation exchange chromatography, phosphocellulose chromatography, hydrophobic interaction chromatography, affinity chromatography, hydroxylapatite chromatography, lectin chromatography and high performance liquid chromatography ("HPLC") is employed for purification.

Polypeptides of the present invention include naturally purified products, products of chemical synthetic procedures, and products produced by recombinant techniques from a prokaryotic or eukaryotic host, including, for example, bacterial, yeast, higher plant, insect and mammalian cells.

Polypeptides and Fragments The invention further provides an isolated E. faecalis polypeptide having an amino acid sequence in Table 1, or a peptide or polypeptide comprising a portion of the above polypeptides.

Variant and Mutant Polypeptides To improve or alter the characteristics of E. faecalis polypeptides of the present invention, protein engineering may be employed. Recombinant DNA technology known to those skilled in the art can be used to create novel mutant proteins or muteins including single or multiple amino acid substitutions, deletions, additions, or fusion proteins. Such modified polypeptides can show, enhanced activity or increased stability. In addition, they may be purified in higher yields and show better solubility than the corresponding natural polypeptide, at least under certain purification and storage conditions.

N-Terminal and C-Terminal Deletion Mutants

S

It is known in the art that one or more amino acids may be deleted from the N-terminus or C-terminus without substantial loss of biological function. For instance, Ron et al. J. Biol. Chem., 268:2984-2988 (1993), reported modified KGF 20 proteins that had heparin binding activity even if 3, 8, or 27 N-terminal amino acid residues were missing. Accordingly, the present invention provides polypeptides **having one or more residues deleted from the amino terminus of the amino acid sequence of the E. faecalis polypeptides shown in Table 1, and polynucleotides encoding such polypeptides.

25 Similarly, many examples of biologically functional C-terminal deletion muteins are known. For instance, Interferon gamma shows up to ten times higher activities by deleting 8-10 amino acid residues from the carboxy terminus of the protein See, Dobeli, et al. (1988) J. Biotechnology 7:199-216. Accordingly, the present invention provides polypeptides having one or more residues from the carboxy terminus of the amino acid sequence of the E.faecalis polypeptides shown in Table 1. The invention also provides polypeptides having one or more amino acids deleted from both the amino and the carboxyl termini as described below.

The present invention is further directed to polynucleotide encoding portions or fragments of the amino acid sequences described herein as well as to portions or fragments of the isolated amino acid sequences described herein. Fragments include portions of the amino acid sequences of Table 1, are at least 5 contiguous amino acid in length, are selected from any two integers, one of which representing a N-terminal position. The initiation codon of the polypeptides of thd present inventions position 1. Every combination of a N-terminal and C-terminal position that a fragment at least contiguous amino acid residues in length could occupy, on any given amino acid sequence of Table 1 is included in the invention. At least means a fragment may be contiguous amino acid residues in length or any integer between 5 and the number of residues in a full length amino acid sequence minus 1. Therefore, included in the 15 invention are contiguous fragments specified by any N-terminal and C-terminal positions of amino acid sequence set forth in Table 1 wherein the contiguous fragment S. is any integer between 5 and the number of residues in a full length sequence minus 1.

Further, the invention includes polypeptides comprising fragments specified by size, in amino acid residues, rather than by N-terminal and C-terminal positions.

The invention includes any fragment size, in contiguous amino acid residues, selected from integers between 5 and the number of residues in a full length sequence minus 1.

Preferred sizes of contiguous polypeptide fragments include about 5 amino acid residues, about 10 amino acid residues, about 20 amino acid residues, about 30 amino acid residues, about 40 amino acid residues, about 50 amino acid residues, about 100

S.

amino acid residues, about 200 amino acid residues, about 300 amino acid residues, and about 400 amino acid residues. The preferred sizes are, of course, meant to exemplify, not limit, the present invention as all size fragments representing any integer between 5 and the number of residues in a full length sequence minus 1 are included in the invention. The present invention also provides for the exclusion of any fragments specified by N-terminal and C-terminal positions or by size in amino acid residues as described above. Any number of fragments specified by N-terminal and C-terminal positions or by size in amino acid residues as described above may be excluded.

The above fragments need not be active since they would be useful, for example, in immunoassays, in epitope mapping, epitope tagging, to generate antibodies to a particular portion of the protein, as vaccines, and as molecular weight markers.

Other Mutants In addition to N- and C-terminal deletion forms of the protein discussed above, it also will be recognized by one of ordinary skill in the art that some amino acid sequences of the E. faecalis polypeptide can be varied without significant effect of the Sb'. structure or function of the protein. If such differences in sequence are contemplated, it should be remembered that there will be critical areas on the protein which determine activity.

Thus, the invention further includes variations of the E. faecalis polypeptides which show substantial E.faecalis polypeptide activity or which include regions of E.

•faecalis protein such as the protein portions discussed below. Such mutants include deletions, insertions, inversions, repeats, and type substitutions selected according to general rules known in the art so as to have little effect on activity. For example, guidance concerning how to make phenotypically silent amino acid substitutions is provided. There are two main approaches for studying the tolerance of an amino acid sequence to change. See, Bowie, J. U. et al. (1990), Science 247:1306-1310. The first method relies on the process of evolution, in which mutations are either accepted or rejected by natural selection. The second approach uses genetic engineering to introduce amino acid changes at specific positions of a cloned gene and selections or screens to identify sequences that maintain functionality.

These studies have revealed that proteins are surprisingly tolerant of amino acid substitutions. The studies indicate which amino acid changes are likely to be permissive at a certain position of the protein. For example, most buried amino acid residues require nonpolar side chains, whereas few features of surface side chains are generally conserved. Other such phenotypically silent substitutions are described by Bowie et al. (supra) and the references cited therein. Typically seen as conservative substitutions are the replacements, one for another, among the aliphatic amino acids Ala, Val, Leu and lie; interchange of the hydroxyl residues Ser and Thr, exchange of the acidic residues Asp and Glu, substitu-tion between the amide residues Asn and Gin, exchange of the basic residues Lys and Arg and replacements among the aromatic residues Phe, Tyr.

Thus, the fragment, derivative, analog, or homolog of the polypeptide of Table 1, or that encoded by the plaimds listed in Table 1, may be: one in which one or more of the amino acid residues are substituted with a conserved or non-conserved 0 amino acid residue (preferably a conserved amino acid residue) and such substituted 15 amino acid residue may or may not be one encoded by the genetic code: or (ii) one in which one or more of the amino acid residues includes a substituent group: or (iii) one 0* in which the E. faecalis polypeptide is fused with another compound, such as a compound to increase the half-life of the polypeptide (for example, polyethylene glycol): or (iv) one in which the additional amino acids are fused to the above form of the polypeptide, such as an IgG Fc fusion region peptide or leader or secretory sequence or a sequence which is employed for purification of the above form of the polypeptide or a proprotein sequence. Such fragments, derivatives and analogs are deemed to be within the scope of those skilled in the art from the teachings herein.

Thus, the E. faecalis polypeptides of the present invention may include one or more amino acid substitutions, deletions, or additions, either from natural mutations or human manipulation. As indicated, changes are preferably of a minor nature, such as conservative amino acid substitutions that do not significantly affect the folding or activity of the protein (see Table 3).

Amino acids in the E. faecalis proteins of the present invention that are essential for function can be identified by methods known in the art, such as sitedirected mutagenesis or alanine-scanning mutagenesis. See, Cunningham et al.

(1989) Science 244:1081-1085. The latter procedure introduces single alanine mutations at every residue in the molecule. The resulting mutant molecules are then tested for biological activity using assays appropriate for measuring the function of the particular protein.

Of special interest are substitutions of charged amino acids with other charged or neutral amino acids which may produce proteins with highly desirable improved characteristics, such as less aggregation. Aggregation may not only reduce activity but also be problematic when preparing pharmaceutical formulations, because aggregates can be immunogenic. See, Pinckard et al., (1967) Clin. Exp. Immunol. 2:331-340; Robbins, et al., (1987) Diabetes 36:838-845; Cleland, et al., (1993) Crit. Rev.

Therapeutic Drug Carrier Systems 10:307-377.

The polypeptides of the present invention are preferably provided in an 15 isolated form, and preferably are substantially purified. A recombinantly produced version of the E. faecalis polypeptide can be substantially purified by the one-step method described by Smith et al. (1988) Gene 67:31-40. Polypeptides of the invention also can be purified from natural or recombinant sources using antibodies 6*oooS directed against the polypeptides of the invention in methods which are well known in the art of protein purification.

The invention further provides for isolated E. faecalis polypeptides comprising an amino acid sequence selected from the group consisting of: the amino acid sequence of a full-length E. faecalis polypeptide having the complete amino acid sequence shown in Table 1; the amino acid sequence of a full-length E.

faecalis polypeptide having the complete amino acid sequence shown in Table I excepting the N-terminal methionine; the complete amino acid sequence encoded by the plaimds listed in Table 1; and the complete amino acid sequence excepting the N-terminal methionine encoded by the plaimds listed in Table 1. The polypeptides of the present invention also include polypeptides having an amino acid sequence at least 80% identical, more preferably at least 90% identical, and still more preferably 95%, 96%, 97%, 98% or 99% identical to those described in and above.

Further polypeptides of the present invention include polypeptides which have at least 90% similarity, more preferably at least 95% similarity, and still more preferably at least 96%, 97%, 98% or 99% similarity to those described above.

A further embodiment of the invention relates to a polypeptide which comprises the amino acid sequence of a E. faecalis polypeptide having an amino acid sequence which contains at least one conservative amino acid substitution, but not more than 50 conservative amino acid substitutions, not more than 40 conservative amino acid substitutions, not more than 30 conservative amino acid substitutions, and not more than 20 conservative amino acid substitutions. Also provided are polypeptides which comprise the amino acid sequence of a E. faecalis polypeptide, having at least one, but not more than 10, 9, 8, 7, 6, 5, 4, 3, 2 or 1 conservative amino 15 acid substitutions.

By a polypeptide having an amino acid sequence at least, for example, "identical" to a query amino acid sequence of the present invention, it is intended that the amino acid sequence of the subject polypeptide is identical to the query sequence except that the subject polypeptide sequence may include up to five amino acid alterations per each 100 amino acids of the query amino acid sequence. In other words, to obtain a polypeptide having an amino acid sequence at least 95% identical to a query amino acid sequence, up to 5% of the amino acid residues in the subject sequence may be inserted, deleted, (indels) or substituted with another amino acid.

;These alterations of the reference sequence may occur at the amino or carboxy terminal positions of the reference amino acid sequence or anywhere between those terminal positions, interspersed either individually among residues in the reference sequence or in one or more contiguous groups within the reference sequence.

As a practical matter, whether any particular polypeptide is at least 96%, 97%, 98% or 99% identical to, for instance, the amino acid sequences shown in Table 1 or to the amino acid sequence encoded by the plaimds listed in Table I can be determined conventionally using known computer programs. A preferred method for determining the best overall match between a query sequence (a sequence of the present invention) and a subject sequence, also referred to as a global sequence alignment, can be determined using the FASTDB computer program based on the algorithm of Brutlag et al., (1990) Comp. App. Biosci. 6:237-245. In a sequence alignment the query and subject sequences are both amino acid sequences. The result of said global sequence alignment is in percent identity. Preferred parameters used in a FASTDB amino acid alignment are: Matrix=PAM 0, k-tuple=2, Mismatch to Penalty=1, Joining Penalty=20, Randomization Group Length=0, Cutoff Score=l, Window Size=sequence length, Gap Penalty=5, Gap Size Penalty=0.05, Window Size=500 or the length of the subject amino acid sequence, whichever is shorter.

If the subject sequence is shorter than the query sequence due to N- or Cterminal deletions, not because of internal deletions, the results, in percent identity, I* must be manually corrected. This is because the FASTDB program does not account for N- and C-terminal truncations of the subject sequence when calculating global percent identity. For subject sequences truncated at the N- and C-termini, relative to the query sequence, the percent identity is corrected by calculating the number of •residues of the query sequence that are N- and C-terminal of the subject sequence, which are not matched/aligned with a corresponding subject residue, as a percent of the total bases of the query sequence. Whether a residue is matched/aligned is determined by results of the FASTDB sequence alignment. This percentage is then subtracted from the percent identity, calculated by the above FASTDB program using the specified parameters, to arrive at a final percent identity score. This final percent identity score is what is used for the purposes of the present invention. Only residues to the N- and C-termini of the subject sequence, which are not matched/aligned with the query sequence, are considered for the purposes of manually adjusting the percent identity score. That is, only query amino acid residues outside the farthest N- and C-terminal residues of the subject sequence.

For example, a 90 amino acid residue subject sequence is aligned with a 100 residue query sequence to determine percent identity. The deletion occurs at the Nterminus of the subject sequence and therefore, the FASTDB alignment does not match/align with the first 10 residues at the N-terminus. The 10 unpaired residues represent 10% of the sequence (number of residues at the N- and C- termini not matched/total number of residues in the query sequence) so 10% is subtracted from the percent identity score calculated by the FASTDB program. If the remaining residues were perfectly matched the final percent identity would be 90%. In another example, a 90 residue subject sequence is compared with a 100 residue query sequence. This time the deletions are internal so there are no residues at the N- or Ctermini of the subject sequence which are not matched/aligned with the query. In this case the percent identity calculated by FASTDB is not manually corrected. Once again, only residue positions outside the N- and C-terminal ends of the subject sequence, as displayed in the FASTDB alignment, which are not matched/aligned 15 with the query sequence are manually corrected. No other manual corrections are to made for the purposes of the present invention.

The above polypeptide sequences are included irrespective of whether they have their normal biological activity. This is because even where a particular polypeptide molecule does not have biological activity, one of skill in the art would 20 still know how to use the polypeptide, for instance, as a vaccine or to generate antibodies. Other uses of the polypeptides of the present invention that do not have E. faecalis activity include, inter alia, as epitope tags, in epitope mapping, and as molecular weight markers on SDS-PAGE gels or on molecular sieve gel filtration columns using methods known to those of skill in the art.

As described below, the polypeptides of the present invention can also be used to raise polyclonal and monoclonal antibodies, which are useful in assays for detecting E. faecalis protein expression or as agonists and antagonists capable of enhancing or inhibiting E. faecalis protein function. Further, such polypeptides can be used in the yeast two-hybrid system to "capture" E. faecalis protein binding proteins which are also candidate agonists and antagonists according to the present invention.

See, Fields et al. (1989) Nature 340:245-246.

Epilope-Bearing Portions In another aspect, the invention provides peptides and polypeptides comprising epitope-bearing portions of the E. faecalis polypeptides of the present invention. These epitopes are immunogenic or antigenic epitopes of the polypeptides of the present invention. An "immunogenic epitope" is defined as a part of a protein that elicits an antibody response when the whole protein or polypeptide is the irmmunogen. These immunogenic epitopes are believed to be confined to a few loci on the molecule. On the other hand, a region of a protein molecule to which an antibody can bind is defined as an "antigenic determinant" or "antigenic epitope." The number of immunogenic epitopes of a protein generally is less than the number of antigenic epitopes. See, Geysen, et al. (1983) Proc. Natl. Acad. Sci. USA 81:3998- 4002.

is' 5 Predicted antigenic epitopes are shown in Table 4, below. It is pointed out that Table 4 only lists amino acid residues comprising epitopes predicted to have the highest degree of antigenicity. The polypeptides not listed in Table 4 and portions of polypeptides not listed in Table 4 are not considered non-antigenic. This is because they may still be antigenic in vivo but merely not recognized as such by the particular algorithm used. Thus, Table 4 lists the amino acid residues comprising preferred antigenic epitopes but not a complete list. Amino acid residues comprising other anigenic epitopes may be determined by algorithms similar to the Jameson-Wolf analysis or by in vivo testing for an antigenic response using the methods described herein or those known in the art.

As to the selection of peptides or polypeptides bearing an antigenic epitope that contain a region of a protein molecule to which an antibody can bind), it is well known in that art that relatively short synthetic peptides that mimic part of a protein sequence are routinely capable of eliciting an antiserum that reacts with the partially mimicked protein. See, Sutcliffe, et al., (1983) Science 219:660-666.

Peptides capable of eliciting protein-reactive sera are frequently represented in the primary sequence of a protein, can be characterized by a set of simple chemical rules, and are confined neither to immunodominant regions of intact proteins immunogenic epitopes) nor to the amino or carboxyl terminals. Peptides that are extremely hydrophobic and those of six or fewer residues generally are ineffective at inducing antibodies that bind to the mimicked protein; longer, peptides, especially those containing proline residues, usually are effective. See, Sutcliffe, et al., supra, p.

661. For instance, 18 of 20 peptides designed according to these guidelines, containing 8-39 residues covering 75% of the sequence of the influenza virus hemagglutinin HA1 polypeptide chain, induced antibodies that reacted with the HAl protein or intact virus; and 12/12 peptides from the MuLV polymerase and 18/18 from the rabies glycoprotein induced antibodies that precipitated the respective proteins.

Antigenic epitope-bearing peptides and polypeptides of the invention are therefore useful to raise antibodies, including monoclonal antibodies, that bind 15 specifically to a polypeptide of the invention. Thus, a high proportion of hybridomas obtained by fusion of spleen cells from donors immunized with an antigen epitope-bearing peptide generally secrete antibody reactive with the native protein.

See Sutcliffe, et al., supra, p. 663. The antibodies raised by antigenic epitope-bearing peptides or polypeptides are useful to detect the mimicked protein, and antibodies to 20 different peptides may be used for tracking the fate of various regions of a protein precursor which undergoes post-translational processing. The peptides and oo• anti-peptide antibodies may be used in a variety of qualitative or quantitative assays for the mimicked protein, for instance in competition assays since it has been shown that even short peptides about 9 amino acids) can bind and displace the larger 25 peptides in immunoprecipitation assays. See, Wilson, et al., (1984) Cell 37:767-778. The anti-peptide antibodies of the invention also are useful for purification of the mimicked protein, for instance, by adsorption chromatography using methods known in the art.

Antigenic epitope-bearing peptides and polypeptides of the invention designed according to the above guidelines preferably contain a sequence of at least seven, more preferably at least nine and most preferably between about 10 to about amino acids any integer between 7 and 50) contained within the amino acid sequence of a polypeptide of the invention. However, peptides or polypeptides comprising a larger portion of an amino acid sequence of a polypeptide of the invention, containing about 50 to about 100 amino acids, or any length up to and including the entire amino acid sequence of a polypeptide of the invention, also are considered epitope-bearing peptides or polypeptides of the invention and also are useful for inducing antibodies that react with the mimicked protein. Preferably, the amino acid sequence of the epitope-bearing peptide is selected to provide substantial solubility in aqueous solvents the sequence includes relatively hydrophilic residues and highly hydrophobic sequences are preferably avoided); and sequences containing proline residues are particularly preferred.

Non-limiting examples of antigenic polypeptides or peptides that can be used to generate an enterococcal-specific immune response or antibodies include portions of the amino acid sequences identified in Table 1. More specifically, Table 4 discloses a list of non-limiting residues that are involved in the antigenicity of the epitope-bearing fragments of the present invention. Therefore, the present inventions provides for isolatd and purified antigenic epitope-bearing fragements of the polypeptides of the present invention comprising a peptide sequences of Table 4. The antigenic epitopebearing fragments comprising a peptide sequence of Table 4 preferably contain a sequence of at least seven, more preferably at least nine and most preferably between about 10 to about 50 amino acids any integer between 7 and 50) of a polypeptide of the present invention. That is, included in the present invention are antigenic polypeptides between the integers of 7 and 50 amino acid in length comprising one or more of the sequences of Table 4. Therefore, in most cases, the polypeptides of Table 4 make up only a portion of the antigenic polypeptide. All combinations of sequences between the integers of 7 and 50 amino acid in length comprising one or more of the sequences of Table 4 are included. The antigenic epitope-bearing fragements may be specified by either the number of contiguous amino acid residues or by specific N-terminal and C-terminal positions as described above for the polypeptide fragements of the present invention, wherein the initiation codon is residue 1. Any number of the described antigenic epitope-bearing fragements of the present invention may also be excluded from the present invention in the same manner.

The epitope-bearing peptides and polypeptides of the invention may be produced by any conventional means for making peptides or polypeptides including recombinant means using nucleic acid molecules of the invention. For instance, an epitope-bearing amino acid sequence of the present invention may be fused to a larger polypeptide which acts as a carrier during recombinant production and purification, as well as during immunization to produce anti-peptide antibodies. Epitope-bearing peptides also may be synthesized using known methods of chemical synthesis. For "instance, Houghten has described a simple method for synthesis of large numbers of 15 peptides, such as 10-20 mg of 248 different 13 residue peptides representing single amino acid variants of a segment of the HA1 polypeptide which were prepared and characterized (by ELISA-type binding studies) in less than four weeks (Houghten, R.

A. Proc. Natl. Acad. Sci. USA 82:5131-5135 (1985)). This "Simultaneous Multiple Peptide Synthesis (SMPS)" process is further described in U.S. Patent No. 4,631,211 to.Houghten and coworkers (1986). In this procedure the individual resins for the solid-phase synthesis of various peptides are contained in separate solvent-permeable packets, enabling the optimal use of the many identical repetitive steps involved in solid-phase methods. A completely manual procedure allows 500-1000 or more syntheses to be conducted simultaneously (Houghten et al. (1985) Proc. Natl. Acad.

Sci. 82:5131-5135 at 5134.

Epitope-bearing peptides and polypeptides of the invention are used to induce antibodies according to methods well known in the art. See, Sutcliffe, et al., supra;; Wilson, et al., supra;; and Bittle, et al. (1985) J. Gen. Virol. 66:2347-2354.

Generally, animals may be immunized with free peptide; however, anti-peptide antibody titer may be boosted by coupling of the peptide to a macromolecular carrier, such as keyhole limpet hemacyanin (KLH) or tetanus toxoid. For instance, peptides containing cysteine may be coupled to carrier using a linker such as m-maleimidobenzoyl-N-hydroxysuccinimide ester (MBS), while other peptides may be coupled to carrier using a more general linking agent such as glutaraldehyde.

Animals such as rabbits, rats and mice are immunized with either free or carrier-coupled peptides, for instance, by intraperitoneal and/or intradermal injection of emulsions containing about 100 p.g peptide or carrier protein and Freund's adjuvant.

Several booster injections may be needed, for instance, at intervals of about two weeks, to provide a useful titer of anti-peptide antibody which can be detected, for example, by ELISA assay using free peptide adsorbed to a solid surface. The titer of anti-peptide antibodies in serum from an immunized animal may be increased by selection of anti-peptide antibodies, for instance, by adsorption to the peptide on a solid support and elution of the selected antibodies according to methods well known in the art.

Immunogenic epitope-bearing peptides of the invention, those parts of a protein that elicit an antibody response when the whole protein is the immunogen, are identified according to methods known in the art. For instance, Geysen, el al., supra, I discloses a procedure for rapid concurrent synthesis on solid supports of hundreds of peptides of sufficient purity to react in an ELISA. Interaction of synthesized peptides with antibodies is then easily detected without removing them from the support. In this manner a peptide bearing an immunogenic epitope of a desired protein may be identified routinely by one of ordinary skill in the art. For instance, the immunologically important epitope in the coat protein of foot-and-mouth disease virus was located by Geysen el al. supra with a resolution of seven amino acids by synthesis of an overlapping set of all 208 possible hexapeptides covering the entire 213 amino acid sequence of the protein. Then, a complete replacement set of peptides in which all 20 amino acids were substituted in turn at every position within the epitope were synthesized, and the particular amino acids conferring specificity for the reaction with antibody were determined. Thus, peptide analogs of the epitope-bearing peptides of the invention can be made routinely by this method. U.S. Patent No.

4,708,781 to Geysen (1987) further describes this method of identifying a peptide bearing an imnnunogenic epitope of a desired protein.

Further still, U.S. Patent No. 5,194,392, to Geysen (1990), describes a general method of detecting or determining the sequence of monomers (amino acids or other compounds) which is a topological equivalent of the epitope a "mimotope") which is complementary to a particular paratope (antigen binding site) of an antibody of interest. More generally, U.S. Patent No. 4,433,092, also to Geysen (1989), describes a method of detecting or determining a sequence of monomers which is a topographical equivalent of a ligand which is complementary to the ligand binding site of a particular receptor of interest. Similarly, U.S. Patent No. 5,480,971 to Houghten, R. A. et al. (1996) discloses linear C 1

-C

7 -alkyl peralkylated oligopeptides and sets and libraries of such peptides, as well as methods for using such oligopeptide sets and 15 libraries for determining the sequence of a peralkylated oligopeptide that preferentially binds to an acceptor molecule of interest. Thus, non-peptide analogs of the epitope-bearing peptides of the invention also can be made routinely by these methods. The entire disclosure of each document cited in this section on "Polypeptides and Fragments" is hereby incorporated herein by reference.

As one of skill in the art will appreciate, the polypeptides of the present invention and the epitope-bearing fragments thereof described above can be combined with parts of the constant domain of immunoglobulins (IgG), resulting in chimeric polypeptides. These fusion proteins facilitate purification and show an increased half-life in vivo. This has been shown, for chimeric proteins consisting of the first two domains of the human CD4-polypeptide and various domains of the constant regions of the heavy or light chains of mammalian immunoglobulins. (EPA 0,394,827; Traunecker et al. (1988) Nature 331:84-86. Fusion proteins that have a disulfide-linked dimeric structure due to the IgG part can also be more efficient in binding and neutralizing other molecules than a monomeric E. faecalis polypeptide or fragment thereof alone. See Fountoulakis et al. (1995) J. Biochem. 270:3958-3964.

Nucleic acids encoding the above epitopes of E. faecalis polypeptides can also be recombined with a gene of interest as an epitope tag to aid in detection and purification of the expressed polypeptide.

Antibodies E. faecalis protein-specific antibodies for use in the present invention can be raised against the intact E. faecalis protein or an antigenic polypeptide fragment thereof, which may be presented together with a carrier protein, such as an albumin, to an animal system (such as rabbit or mouse) or, if it is long enough (at least about amino acids), without a carrier.

As used herein, the term "antibody" (Ab) or "monoclonal antibody" (Mab) is meant to include intact molecules, single chain whole antibodies, and antibody fragments. Antibody fragments of the present invention include Fab and F(ab')2 and other fragments including single-chain Fvs (scFv) and disulfide-linked Fvs (sdFv).

"Also included in the present invention are chimeric and humanized monoclonal antibodies and polyclonal antibodies specific for the polypeptides of the present invention. The antibodies of the present invention may be prepared by any of a variety of methods. For example, cells expressing a polypeptide of the present invention or an antigenic fragment thereof can be administered to an animal in order to induce the production of sera containing polyclonal antibodies. For example, a preparation of E. faecalis polypeptide or fragment thereof is prepared and purified to render it substantially free of natural contaminants. Such a preparation is then introduced into an animal in order to produce polyclonal antisera of greater specific activity.

In a preferred method, the antibodies of the present invention are monoclonal antibodies or binding fragments thereof. Such monoclonal antibodies can be prepared using hybridoma technology. See, Harlow et al., ANTIBODIES: A LABORATORY MANUAL, (Cold Spring Harbor Laboratory Press, 2nd ed. 1988); Hammerling, et al., in: MONOCLONAL ANTIBODIES AND T-CELL HYBRIDOMAS 563-681 (Elsevier, 1981). Fab and F(ab')2 fragments may be produced by proteolytic cleavage, using enzymes such as papain (to produce Fab fragments) or pepsin (to produce F(ab')2 fragments). Alternatively, E. faecalis polypeptide-binding fragments, chimeric, and humanized antibodies can be produced through the application of recombinant DNA technology or through synthetic chemistry using methods known in the art.

Alternatively, additional antibodies capable of binding to the polypeptide antigen of the present invention may be produced in a two-step procedure through the use of anti-idiotypic antibodies. Such a method makes use of the fact that antibodies are themselves antigens, and that, therefore, it is possible to obtain an antibody which binds to a second antibody. In accordance with this method, E. faecalis polypeptide-specific antibodies are used to immunize an animal, preferably a mouse.

The splenocytes of such an animal are then used to produce hybridoma cells, and the 15 hybridoma cells are screened to identify clones which produce an antibody whose ability to bind to the E. faecalis polypeptide-specific antibody can be blocked by the E. faecalis polypeptide antigen. Such antibodies comprise anti-idiotypic antibodies to the E.faecalis polypeptide-specific antibody and can be used to immunize an animal *to induce formation of further E. faecalis polypeptide-specific antibodies.

20 Antibodies and fragements thereof of the present invention may be described by the portion of a polypeptide of the present invention recognized or specifically bound by the antibody. Antibody binding fragements of a polypeptide of the present invention may be described or specified in the same manner as for polypeptide fragements discussed above., i.e, by N-terminal and C-terminal positions or by size in contiguous amino acid residues. Any number of antibody binding fragments, of a polypeptide of the present invention, specified by N-terminal and C-terminal positions or by size in amino acid residues, as described above, may also be excluded from the present invention. Therefore, the present invention includes antibodies the specifically bind a particuarlly discribed fragement of a polypeptide of the present invention and allows for the exclusion of the same.

Antibodies and fragements thereof of the present invention may also be described or specified in terms of their cross-reactivity. Antibodies and fragements that do not bind polypeptides of any other species of Enterococcus other than E.

faecalis are included in the present invention. Likewise, antibodies and fragements that bind only species of Enterococcus, i.e. antibodies and fragements that do not bind bacteria from any genus other than Enterococcus, are included in the present invention.

Diagnostic Assays The present invention further relates to methods for assaying staphylococcal infection in an animal by detecting the expression of genes encoding staphylococcal polypeptides of the present invention. The methods comprise analyzing tissue or body fluid from the animal for Enterococcus-specific antibodies, nucleic acids, or proteins. Analysis of nucleic acid specific to Enterococcus is assayed by PCR or hybridization techniques using nucleic acid sequences of the present invention as either hybridization probes or primers. See, Sambrook et al. Molecular cloning: A Laboratory Manual (Cold Spring Harbor Laboratory Press, 2nd ed., 1989, page 54 *:reference); Eremeeva et al. (1994) J. Clin. Microbiol. 32:803-810 (describing differentiation among spotted fever group Rickettsiae species by analysis of restriction fragment length polymorphism of PCR-amplified DNA) and Chen et al. 1994 J. Clin.

Microbiol. 32:589-595 (detecting B. burgdorferi nucleic acids via PCR).

Where diagnosis of a disease state related to infection with Enterococcus has already been made, the present invention is useful for monitoring progression or regression of the disease state whereby patients exhibiting enhanced Enterococcus gene expression will experience a worse clinical outcome relative to patients expressing these gene(s) at a lower level.

By "biological sample" is intended any biological sample obtained from an animal, cell line, tissue culture, or other source which contains Enterococcus polypeptide, mRNA, or DNA. Biological samples include body fluids (such as saliva, blood, plasma, urine, mucus, synovial fluid, etc.) tissues (such as muscle, skin, and cartilage) and any other biological source suspected of containing Enterococcus polypeptides or nucleic acids. Methods for obtaining biological samples such as tissue are well known in the art.

The present invention is useful for detecting diseases related to Enterococcus infections in animals. Preferred animals include monkeys, apes, cats, dogs, birds, cows, pigs, mice, horses, rabbits and humans. Particularly preferred are humans.

Total RNA can be isolated from a biological sample using any suitable technique such as the single-step guanidinium-thiocyanate-phenol-chloroform method described in Chomczynski et al. (1987) Anal. Biochem. 162:156-159. mRNA encoding Enterococcus polypeptides having sufficient homology to the nucleic acid sequences identified in Table 1 to allow for hybridization between complementary sequences are then assayed using any appropriate method. These include Northern blot analysis, S 1 nuclease mapping, the polymerase chain reaction (PCR), reverse transcription in combination with the polymerase chain reaction (RT-PCR), and reverse transcription in combination with the ligase chain reaction (RT-LCR).

Northern blot analysis can be performed as described in Harada et al. (1990) Cell 63:303-312. Briefly, total RNA is prepared from a biological sample as described above. For the Northern blot, the RNA is denatured in an appropriate buffer (such as glyoxal/dimethyl sulfoxide/sodium phosphate buffer), subjected to agarose gel electrophoresis, and transferred onto a nitrocellulose filter. After the RNAs have been linked to the filter by a UV linker, the filter is prehybridized in a solution containing formamide, SSC, Denhardt's solution, denatured salmon sperm, SDS, and sodium 25 phosphate buffer. A E. faecalis polynucleotide sequence shown in Table 1 labeled according to any appropriate method (such as the 2 P-multiprimed DNA labeling system (Amersham)) is used as probe. After hybridization overnight, the filter is washed and exposed to x-ray film. DNA for use as probe according to the present invention is described in the sections above and will preferably at least 15 nucleotides in length.

S1 mapping can be performed as described in Fujita et al. (1987) Cell 49:357-367. To prepare probe DNA for use in SI mapping, the sense strand of an above-described E.faecalis DNA sequence of the present invention is used as a template to synthesize labeled antisense DNA. The antisense DNA can then be digested using an appropriate restriction endonuclease to generate further DNA probes of a desired length. Such antisense probes are useful for visualizing protected bands corresponding to the target mRNA mRNA encoding Enterococcus polypeptides).

Levels of mRNA encoding Enterococcus polypeptides are assayed, for e.g., using the RT-PCR method described in Makino ct al. (1990) Technique 2:295-301.

By this method, the radioactivities of the "amplicons" in the polyacrylamide gel bands are linearly related to the initial concentration of the target mRNA. Briefly, this method involves adding total RNA isolated from a biological sample in a reaction 15 mixture containing a RT primer and appropriate buffer. After incubating for primer annealing, the mixture can be supplemented with a RT buffer, dNTPs, DTT, RNase inhibitor and reverse transcriptase. After incubation to achieve reverse transcription of the RNA, the RT products are then subject to PCR using labeled primers.

Alternatively, rather than labeling the primers, a labeled dNTP can be included in the 2 PCR reaction mixture. PCR amplification can be performed in a DNA thermal cycler according to conventional techniques. After a suitable number of rounds to achieve amplification, the PCR reaction mixture is electrophoresed on a polyacrylamide gel.

After drying the gel, the radioactivity of the appropriate bands (corresponding to the mRNA encoding the Enterococcus polypeptides of the present invention) are 25 quantified using an imaging analyzer. RT and PCR reaction ingredients and conditions, reagent and gel concentrations, and labeling methods are well known in the art. Variations on the RT-PCR method will be apparent to the skilled artisan. Other PCR methods that can detect the nucleic acid of the present invention can be found in PCR PRIMER: A LABORATORY MANUAL Dieffenbach et al. eds., Cold Spring Harbor Lab Press, 1995).

The polynucleotides of the present invention, including both DNA and RNA, may be used to detect polynucleotides of the present invention or Enterococcal species including E. faecalis using bio chip technology. The present invention includes both high density chip arrays (>1000 oligonucleotides per cm 2 and low density chip arrays (<1000 oligonucleotides per cm 2 Bio chips comprising arrays of polynucleotides of the present invention may be used to detect Enterococcal species, including E. faecalis, in biological and environmental samples and to diagnose an animal, including humans, with an E. faecalis or other Enterococcal infection. The bio chips of the present invention may comprise polynucleotide sequences of other pathogens including bacteria, viral, parasitic, and fungal polynucleotide sequences, in addition to the polynucleotide sequences of the present invention, for use in rapid diffenertial pathogenic detection and diagnosis. The bio chips can also be used to monitor an E. faecalis or other Enterococcal infections and to monitor the genetic 15 changes (deletions, insertions, mismatches, etc.) in response to drug therapy in the "clinic and drug development in the laboratory. The bio chip technology comprising arrays of polynucleotides of the present invention may also be used to simultaneously monitor the expression of a multiplicity of genes, including those of the present invention. The polynucleotides used to comprise a selected array may be specified in the same manner as for the fragements, i.e, by their 5' and 3' positions or length in contigious base pairs and include from. Methods and particular uses of the polynucleotides of the present invention to detect Enterococcal species, including E.

faecalis, using bio chip technology include those known in the art and those of: U.S.

Patent Nos. 5510270, 5545531, 5445934, 5677195, 5532128, 5556752, 5527681, 25 5451683, 5424186, 5607646, 5658732 and World Patent Nos. WO/9710365, WO/9511995, WO/9743447, WO/9535505, each incorporated herein in their entireties.

Biosensors using the polynucleotides of the present invention may also be used to detect, diagnose, and monitor E. faecalis or other Enterococcal species and infections thereof. Biosensors using the polynucleotides of the present invention may also be used to detect particular polynucleotides of the present invention. Biosensors using the polynucleotides of the present invention may also be used to monitor the genetic changes (deletions, insertions, mismatches, etc.) in response to drug therapy in the clinic and drug development in the laboratory. Methods and particular uses of the polynucleotides of the present invention to detect Enterococcal species, including E.

faecalis, using biosenors include those known in the art and those of: U.S. Patent Nos 5721102, 5658732, 5631170, and WorldPatentNos. W097/35011, WO/9720203, each incorporated herein in their entireties.

Thus, the present invention includes both bio chips and biosensors comprising polynucleotides of the present invention and methods of their use.

Assaying Enterococcus polypeptide levels in a biological sample can occur using any art-known method, such as antibody-based techniques. For example, Enterococcus polypeptide expression in tissues can be studied with classical 15 immunohistological methods. In these, the specific recognition is provided by the primary antibody (polyclonal or monoclonal) butthe secondary detection system can utilize fluorescent, enzyme, or other conjugated secondary antibodies. As a result, an immunohistological staining of tissue section for pathological examination is obtained.

Tissues can also be extracted, with urea and neutral detergent, for the liberation of

S

Enterococcus polypeptides for Western-blot or dot/slot assay. See, Jalkanen, M.

etal. (1985) J. Cell. Biol. 101:976-985; Jalkanen, M. et al. (1987) J. Cell. Biol.

105:3087-3096. In this technique, which is based on the use of cationic solid phases, quantitation of a Enterococcus polypeptide can be accomplished using an isolated Enterococcus polypeptide as a standard. This technique can also be applied to body 25 fluids.

Other antibody-based methods useful for detecting Enterococcus polypeptide gene expression include immunoassays, such as the ELISA and the radioimmunoassay (RIA). For example, a Enterococcus polypeptide-specific monoclonal antibodies can be used both as an immunoabsorbent and as an enzyme-labeled probe to detect and quantify a Enterococcus polypeptide. The amount of a Enierococcus polypeptide present in the sample can be calculated by reference to the amount present in a standard preparation using a linear regression computer algorithm. Such an ELISA is described in lacobelli et al. (1988) Breast Cancer Research and Treatment 11:19-30. In another ELISA assay, two distinct specific monoclonal antibodies can be used to detect Enterococcus polypeptides in a body fluid. In this assay, one of the antibodies is used as the immunoabsorbent and the other as the enzyme-labeled probe.

The above techniques may be conducted essentially as a "one-step" or "two-step" assay. The "one-step" assay involves contacting the Enterococcus to polypeptide with irmnobilized antibody and, without washing, contacting the mixture with the labeled antibody. The "two-step" assay involves washing before contacting the mixture with the labeled antibody. Other conventional methods may also be employed as suitable. It is usually desirable to immobilize one component of the assay system on a support, thereby allowing other components of the system to be 15 brought into contact with the component and readily removed from the sample.

Variations of the above and other immunological methods included in the present invention can also be found in Harlow et al., ANTIBODIES: A LABORATORY *so MANUAL, (Cold Spring Harbor Laboratory Press, 2nd .ed. 1988).

Suitable enzyme labels include, for example, those from the oxidase group, 0 20 which catalyze the production of hydrogen peroxide by reacting with substrate.

Glucose oxidase is particularly preferred as it has good stability and its substrate (glucose) is readily available. Activity of an oxidase label may be assayed by measuring the concentration of hydrogen peroxide formed by the enzyme-labeled antibody/substrate reaction. Besides enzymes, other suitable labels include 25 radioisotopes, such as iodine (125, 1211), carbon 4 sulphur (35S), tritium 3

H),

indium 1 2 1n), and technetium and fluorescent labels, such as fluorescein and rhodamine, and biotin.

Further suitable labels for the Enterococcus polypeptide-specific antibodies of the present invention are provided below. Examples of suitable enzyme labels include malate dehydrogenase, Enterococcal nuclease, delta-5-steroid isomerase, yeast-alcohol dehydrogenase, alpha-glycerol phosphate dehydrogenase, triose phosphate isomerase, peroxidase, alkaline phosphatase, asparaginase, glucose oxidase, beta-galactosidase, ribonuclease, urease, catalase, glucose-6-phosphate dehydrogenase, glucoamylase, and acetylcholine esterase.

Examples of suitable radioisotopic labels include 3 H, 1251, 1311 32 P, 35

S,

14C, 51 Cr, sTTo, 5 sCo, 59 Fe, 75 Se, 5 2 Eu, 90Y, 67 Cu, 217 Ci, 21'At, 212 Pb, 4 7Sc, '1Pd, etc.

In is a preferred isotope where in vivo imaging is used since its avoids the problem of dehalogenation of the 1251 or 31 -labeled monoclonal antibody by the liver. In addition, this radionucleotide has a more favorable gamma emission energy for imaging.

See, Perkins et al. (1985) Eur. J. Nucl. Med. 10:296-301; Carasquillo et al.

(1987) J. Nucl. Med. 28:281-287. For example, "In coupled to monoclonal antibodies with -(P-isothiocyanatobenzyl)-DPTA has shown little uptake in non-tumors tissues, particularly the liver, and therefore enhances specificity of tumor 15 localization. See, Esteban et al. (1987) J. Nucl. Med. 28:861-870.

Examples of suitable non-radioactive isotopic labels include 57 Gd, "Mn, i 62 Dy, 52 Tr, and 5 6 Fe.

Examples of suitable fluorescent labels include an 5 2 Eu label, a fluorescein label, an isothiocyanate label, a rhodamine label, a phycoerythrin label, a phycocyanin label, an allophycocyanin label, an o-phthaldehyde label, and a fluorescamine label.

Examples of suitable toxin labels include, Pseudomonas toxin, diphtheria toxin, ricin, and cholera toxin.

Examples of chemiluminescent labels include a luminal label, an isoluminal label, an aromatic acridinium ester label, an imidazole label, an acridinium salt label, an oxalate ester label, a luciferin label, a luciferase label, and an aequorin label.

Examples of nuclear magnetic resonance contrasting agents include heavy metal nuclei such as Gd, Mn, and iron.

Typical techniques for binding the above-described labels to antibodies are provided by Kennedy et al. (1976) Clin. Chim. Acta 70:1-31, and Schurs et al. (1977) Clin. Chim. Acta 81:1-40. Coupling techniques mentioned in the latter are the glutaraldehyde method, the periodate method, the dimaleimide method, the m-maleimidobenzyl-N-hydroxy-succinimide ester method, all of which methods are incorporated by reference herein.

In a related aspect, the invention includes a diagnostic kit for use in screening serum containing antibodies specific against E. faecalis infection. Such a kit may include an isolated E. faecalis antigen comprising an epitope which is specifically immunoreactive with at least one anti-E.faecalis antibody. Such a kit also includes means for detecting the binding of said antibody to the antigen. In specific embodiments, the kit may include a recombinantly produced or chemically synthesized peptide or polypeptide antigen. The peptide or polypeptide antigen may be attached to a solid support.

In a more specific embodiment, the detecting means of the above-described kit includes a solid support to which said peptide or polypeptide antigen is attached.

Such a kit may also include a non-attached reporter-labeled anti-human antibody. In this embodiment, binding of the antibody to the E. faecalis antigen can be detected by binding of the reporter labeled antibody to the anti-E. faecalis polypeptide antibody.

In a related aspect, the invention includes a method of detecting E. faecalis infection in a subject. This detection method includes reacting a body fluid, preferably 20 serum, from the subject with an isolated E. faecalis antigen, and examining the antigen for the presence of bound antibody. In a specific embodiment, the method includes a polypeptide antigen attached to a solid support, and serum is reacted with the support. Subsequently, the support is reacted with a reporter-labeled anti-human antibody. The support is then examined for the presence of reporter-labeled antibody.

The solid surface reagent employed in the above assays and kits is prepared by known techniques for attaching protein material to solid support material, such as polymeric beads, dip sticks, 96-well plates or filter material. These attachment methods generally include non-specific adsorption of the protein to the support or covalent attachment of the protein typically through a free amine group, to a chemically reactive group on the solid support, such as an activated carboxyl, hydroxyl, or aldehyde group. Alternatively, streptavidin coated plates can be used in conjunction with biotinylated antigen(s).

The polypeptides and antibodies of the present invention, including fragments thereof, may be used to detect Enterococcal species including E. faecalis using bio chip and biosensor technology. Bio chip and biosensors of the present invention may comprise the polypeptides of the present invention to detect antibodies, which specifically recognize Enterococcal species, including E. faecalis. Bio chip and biosensors of the present invention may also comprise antibodies which specifically recognize the polypeptides of the present invention to detect Enterococcal species, including E. faecalis or specific polypeptides of the present invention. Bio chips or biosensors comprising polypeptides or antibodies of the present invention may be "used todetect Enterococcal species, including E. faecalis, in biological and S. 15 environmental samples and to diagnose an animal, including humans, with an E.

faecalis or other Enterococcal infection. Thus, the present invention includes both bio "chips and biosensors comprising polypeptides or antibodies of the present invention oo and methods of their use.

The bio chips of the present invention may further comprise polypeptide sequences of other pathogens including bacteria, viral, parasitic, and fungal polypeptide sequences, in addition to the polypeptide sequences of the present invention, for use in rapid diffenertial pathogenic detection and diagnosis. The bio chips of the present invention may further comprise antibodies or fragements thereof specific for other pathogens including bacteria, viral, parasitic, and fungal polypeptide sequences, in addition to the antibodies or fragements thereof of the present invention, for use in rapid diffenertial pathogenic detection and diagnosis. The bio chips and biosensors of the present invention may also be used to monitor an E. faecalis or other Enterococcal infection and to monitor the genetic changes (amio acid deletions, insertions, substitutions, etc.) in response to drug therapy in the clinic and drug development in the laboratory. The bio chip and biosensors comprising polypeptides or antibodies of the present invention may also be used to simultaneously monitor the expression of a multiplicity of polypeptides, including those of the present invention.

The polypeptides used to comprise a bio chip or biosensor of the present invention may be specified in the same manner as for the fragements, i.e, by their N-tenninal and C-terminal positions or length in contigious amino acid residue. Methods and particular uses of the polypeptides and antibodies of the present invention to detect Enterococcal species, including E. faecalis, or specific polypeptides using bio chip and biosensor technology include those known in the art, those of the U.S. Patent Nos.

and World Patent Nos. listed above for bio chips and biosensors using polynucleotides of the present invention, and those of: U.S. Patent Nos. 5658732, 5135852, 5567301, 5677196, 5690894 and World Patent Nos. W09729366, 1 W09612957, each incorporated herein in their entireties.

15 Treatment: Agonists and Antagonists Assays and Molecules.

The invention also provides a method of screening compounds to identify those which enhance or block the biological activity of the E. faecalis polypeptides of the present invention. The present invention further provides where the compounds kill or slow the growth of E.faecalis. The ability of E.faecalis antagonists, including E. faecalis ligands, to prophylactically or therapeutically block antibiotic resistance may be easily tested by the skilled artisan. See, Straden et al. (1997) J Bacteriol.

179(1):9-16.

An agonist is a compound which increases the natural biological function or which functions in a manner similar to the polypeptides of the present invention, while antagonists decrease or eliminate such functions. Potential antagonists include small organic molecules, peptides, polypeptides, and antibodies that bind to a polypeptide of the invention and thereby inhibit or extinguish its activity.

The antagonists may be employed for instance to inhibit peptidoglycan cross bridge formation. Antibodies against E. faecalis may be employed to bind to and inhibit E. faecalis activity to treat antibiotic resistance. Any of the above antagonists may be employed in a composition with a pharmaceutically acceptable carrier.

Vaccines The present invention also provides vaccines comprising one or more polypeptides of the present invention. Heterogeneity in the composition of a vaccine may be provided by combining E. faecalis polypeptides of the present invention.

Multi-component vaccines of this type are desirable becautse they are likely to be more effective in eliciting protective immune responses against multiple species and strains of the Enterococcus genus than single polypeptide vaccines.

Multi-component vaccines are known in the art to elicit antibody production to numerous immunogenic components. See, Decker et al. (1996) J. Infect. Dis.

174:S270-275. In addition, a hepatitis B, diphtheria, tetanus, pertussis tetravalent 15 vaccine has recently been demonstrated to elicit protective levels of antibodies in human infants against all four pathogenic agents. See, Aristegui, J. et al. (1997) Vaccine 15:7-9.

The present invention in addition to single-component vaccines includes multi-component vaccines. These vaccines comprise more than one polypeptide, o. 20 immunogen or antigen. Thus, a multi-component vaccine would be a vaccine comprising more than one of the E. faecalis polypeptides of the present invention.

Further within the scope of the invention are whole cell and whole viral vaccines. Such vaccines may be produced recombinantly and involve the expression of one or more of the E. faecalis polypeptides described in Table 1. For example, the E. faecalis polypeptides of the present invention may be either secreted or localized intracellular, on the cell surface, or in the periplasmic space. Further, when a recombinant virus is used, the E. faecalis polypeptides of the present invention may, for example, be localized in the viral envelope, on the surface of the capsid, or internally within the capsid. Whole cells vaccines which employ cells expressing heterologous proteins are known in the art. See, Robinson, K. et al. (1997) Nature Biotech. 15:653-657; Sirard, J. et al. (1997) Infect. Immun. 65:2029-2033; Chabalgoity, J. et al. (1997) Infect. Immun. 65:2402-2412 These cells may be administered live or may be killed prior to administration. Chabalgoity, J. et al., supra, for example, report the successful use in mice of a live attenuated Sahnonella vaccine strain which expresses a portion of a platyhelminth fatty acid-binding protein as a fusion protein on its cells surface.

A multi-component vaccine can also be prepared using techniques known in the art by combining one or more E. faecalis polypeptides of the present invention, or fragments thereof, with additional non-Enterococcal components diphtheria toxin or tetanus toxin, and/or other compounds known to elicit an immune response).

Such vaccines are useful for eliciting protective immune responses to both members of the Enterococcus genus and non-Enterococcal pathogenic agents.

The vaccines of the present invention also include DNA vaccines. DNA vaccines are currently being developed for a number of infectious diseases. See, et al., Boyer, et al. (1997) Nat. Med. 3:526-532; reviewed in Spier, R. (1996) Vaccine 14:1285-1288. Such DNA vaccines contain a nucleotide sequence encoding one or more E. faecalis polypeptides of the present invention oriented in a manner that allows for expression of the subject polypeptide. For example, the direct administration of plasmid DNA encoding B. burgdorgeri OspA has been shown to elicit protective immunity in mice against borrelial challenge. See, Luke et al. (1997) J.

Infect. Dis. 175:91-97.

"The present invention also relates to the administration of a vaccine which is co-administered with a molecule capable of modulating immune responses. Kim et al.

(1997) Nature Biotech. 15:641-646, for example, report the enhancement of immune responses produced by DNA immunizations when DNA sequences encoding molecules which stimulate the immune response are co-administered. In a similar fashion, the vaccines of the present invention may be co-administered with either nucleic acids encoding immune modulators or the immune modulators themselves.

These immune modulators include granulocyte macrophage colony stimulating factor (GM-CSF) and CD86.

The vaccines of the present invention may be used to confer resistance to Enterococcal infection by either passive or active immunization. When the vaccines of the present invention are used to confer resistance to Enterococcal infection through active immunization, a vaccine of the present invention is administered to an animal to elicit a protective immune response which either prevents or attenuates a Enterococcal infection. When the vaccines of the present invention are used to confer resistance to Enterococcal infection through passive immunization, the vaccine is provided to a host animal human, dog, or mouse), and the antisera elicited by this antisera is recovered and directly provided to a recipient suspected of having an infection caused S. by a member of the Enterococcus genus.

The ability to label antibodies, or fragments of antibodies, with toxin molecules provides an additional method for treating Enterococcal infections when passive immunization is conducted. In this embodiment, antibodies, or fragments of antibodies, capable of recognizing the E. faecalispolypeptides disclosed herein, or fragments thereof, as well as other Enterococcus proteins, are labeled with toxin molecules prior to their administration to the patient. When such toxin derivatized antibodies bind to Enterococcus cells, toxin moieties will be localized to these cells and 20 will cause their death.

The present invention thus concerns and provides a means for preventing or attenuating a Enterococcal infection resulting from organisms which have antigens that are recognized and bound by antisera produced in response to the polypeptides of the present invention. As used herein, a vaccine is said to prevent or attenuate a disease if its administration to an animal results either in the total or partial attenuation suppression) of a symptom or condition of the disease, or in the total or partial immunity of the animal to the disease.

The administration of the vaccine (or the antisera which it elicits) may be for either a "prophylactic" or "therapeutic" purpose. When provided prophylactically, the compound(s) are provided in advance of any symptoms of Enterococcal infection.

The prophylactic administration of the compound(s) serves to prevent or attenuate any subsequent infection. When provided therapeutically, the compound(s) is provided upon or after the detection of symptoms which indicate that an animal may be infected with a member of the Enterococcus genus. The therapeutic administration of the compound(s) serves to attenuate any actual infection. Thus, the E. faecalis polypeptides, and fragments thereof, of the present invention may be provided either prior to the onset of infection (so as to prevent or attenuate an anticipated infection) or after the initiation of an actual infection.

The polypeptides of the invention, whether encoding a portion of a native protein or a functional derivative thereof, may be administered in pure form or may be coupled to a macromolecular carrier. Example of such carriers are proteins and carbohydrates. Suitable proteins which may act as macromolecular carrier for enhancing the immunogenicity of the polypeptides of the present invention include keyhole limpet hemacyanin (KLH) tetanus toxoid, pertussis toxin, bovine serum albumin, and ovalbumin. Methods for coupling the polypeptides of the present invention to such macromolecular carriers are disclosed in Harlow et al., ANTIBODIES: A LABORATORY MANUAL, (Cold Spring Harbor Laboratory Press, 2nd ed. 1988).

20 A composition is said to be "pharmacologically or physiologically acceptable" if its administration can be tolerated by a recipient animal and is otherwise suitable for administration to that animal. Such an agent is said to be administered in a "therapeutically effective amount" if the amount administered is physiologically significant. An agent is physiologically significant if its presence results in a detectable change in the physiology of a recipient patient.

While in all instances the vaccine of the present invention is administered as a pharmacologically acceptable compound, one skilled in the art would recognize that the composition of a pharmacologically acceptable compound varies with the animal to which it is administered. For example, a vaccine intended for human use will generally not be co-administered with Freund's adjuvant. Further, the level of purity of the E. faecalis polypeptides of the present invention will normally be higher when administered to a human than when administered to a non-human animal.

As would be understood by one of ordinary skill in the art, when the vaccine of the present invention is provided to an animal, it may be in a composition which may contain salts, buffers, adjuvants, or other substances which are desirable for improving the efficacy of the composition. Adjuvants are substances that can be used to specifically augment a specific immune response. These substances generally perform two functions: they protect the antigen(s) frdm being rapidly catabolized after administration and they nonspecifically stimulate immune responses.

Normally, the adjuvant and the composition are mixed prior to presentation to the immune system, or presented separately, but into the same site of the animal being immunized. Adjuvants can be loosely divided into several groups based upon their composition. These groups include oil adjuvants (for example, Freund's complete and 15 incomplete), mineral salts (for example, AIK(S0 4 2 AINa(SO4) 2

AINH

4 (SO4), silica, kaolin, and carbon), polynucleotides (for example, poly IC and poly AU acids), and certain natural substances (for example, wax D from Mycobacterium tuberculosis, as well as substances found in Corynebacterium parvum, or Bordetella pertussis, and members of the genus Brucella. Other substances useful as adjuvants are the saponins 20 such as, for example, Quil A. (Superfos A/S, Denmark). Preferred adjuvants for use in the present invention include aluminum salts, such as AIK(SO 4 2 AINa(SO 4 2 and

AINH

4 (S0 4 Examples of materials suitable for use in vaccine compositions are provided in REMINGTON'S PHARMACEUTICAL SCIENCES 1324-1341 (A.

Osol, ed, Mack Publishing Co, Easton, PA, (1980) (incorporated herein by reference).

The therapeutic compositions of the present invention can be administered parenterally by injection, rapid infusion, nasopharyngeal absorption (intranasopharangeally), dermoabsorption, or orally. The compositions may alternatively be administered intramuscularly, or intravenously. Compositions for parenteral administration include sterile aqueous or non-aqueous solutions, suspensions, and emulsions. Examples of non-aqueous solvents are propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable organic esters such as ethyl oleate. Carriers or occlusive dressings can be used to increase skin permeability and enhance antigen absorption. Liquid dosage forms for oral administration may generally comprise a liposome solution containing the liquid dosage form. Suitable forms for suspending liposomes include emulsions, suspensions, solutions, syrups, and elixirs containing inert diluents commonly used in the art, such as purified water. Besides the inert diluents, such compositions can also include adjuvants, wetting agents, emulsifying and suspending agents, or sweetening, I o flavoring, or perfuming agents.

Therapeutic compositions of the present invention can also be administered in encapsulated form. For example, intranasal immunization using vaccines encapsulated in biodegradable microsphere composed of poly(DL-lactide-co-glycolide). See, Shahin, R. et al. (1995) Infect. Immun. 63:1195-1200. Similarly, orally administered encapsulated Salmonella typhimurium antigens can also be used. Allaoui-Attarki, K.

et al. (1997) Infect. Immun. 65:853-857. Encapsulated vaccines of the present invention can be administered by a variety of routes including those involving contacting the vaccine with mucous membranes intranasally, intracolonicly, Sintraduodenally).

S 20 Many different techniques exist for the timing of the immunizations when a multiple administration regimen is utilized. It is possible to use the compositions of the invention more than once to increase the levels and diversities of expression of the immunoglobulin repertoire expressed by the immunized animal. Typically, if multiple immunizations are given, they will be given one to two months apart.

According to the present invention, an "effective amount" of a therapeutic composition is one which is sufficient to achieve a desired biological effect. Generally, the dosage needed to provide an effective amount of the composition will vary depending upon such factors as the animal's or human's age, condition, sex, and extent of disease, if any, and other variables which can be adjusted by one of ordinary skill in the art.

The antigenic preparations of the invention can be administered by either single or multiple dosages of an effective amount. Effective amounts of the compositions of the invention can vary from 0.01-1,000 ig/ml per dose, more preferably 0.1-500 p.g/ml per dose, and most preferably 10-300 gg/ml per dose.

Examples Example 1: Isolation of a Selected DNA Clone From the Deposited Sample ofE.

faecalis Three approaches can be used to isolate a E.faecalis clone comprising a polynucleotide of the present invention from any E. faecalis genomic DNA library.

The E.faecalis strain V586 has been deposited as a convienent source for obtaining a E. faecalis strain although a wide varity of strains E. faecalis strains can be used which are known in the art.

E. faecalis genomic DNA is prepared using the following method. A overnight bacterial culture grown in a rich medium Trypticase Soy Broth, Brain Heart Infusion broth or Super broth), pelleted, washed two times with TES Tris-pH 8.0, 25mM EDTA, 50mM NaCI), and resuspended in 5ml high salt TES NaCI). Lysostaphin is added to final concentration of approx 50ug/ml and the 20 mixture is rotated slowly 1 hour at 37C to make protoplast cells. The solution is then placed in incubator (or place in a shaking water bath) and warmed to 55C. Five hundred micro liter of 20% sarcosyl in TES (final concentration is then added to lyse the cells. Next, guanidine HCI is added to a final concentration of 7M (3.69g in ml). The mixture is swirled slowly at 55C for 60-90 min (solution should clear).

A CsCI gradient is then set up in SW41 ultra clear tubes using 2.0ml 5.7M CsCI and overlaying with 2.85M CsCI. The gradient is carefully overlayed with the DNAcontaining GuHCI solution. The gradient is spun at 30,000 rpm, 20C for 24 hr and the lower DNA band is collected. The volume is increased to 5 ml with TE buffer.

The DNA is then treated with protease K (10 ug/ml) overnight at 37 C, and precipitated with ethanol. The precipitated DNA is resuspended in a desired buffer.

In the first method, a plasmid is directly isolated by screening a plasmid E.

faecalis genomic DNA library using a polynucleotide probe corresponding to a polynucleotide of the present invention. Particularly, a specific polynucleotide with 30-40 nucleotides is synthesized using an Applied Biosystems DNA synthesizer according to the sequence reported. The oligonucleotide is labeled, for instance, with 32P-y-ATP using T4 polynucleotide kinase and purified according to routine methods.

(See, Maniatis et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Press, Cold Spring, NY (1982).) The library is transformed into a suitable host, as indicated above (such as XL-I Blue (Stratagene)) using techniques known to those of skill in the art. See, Sambrook et al. MOLECULAR CLONING: A LABORATORY MANUAL (Cold Spring Harbor, N.Y. 2nd ed. 1989); Ausubel et al., CURRENT PROTOCALS IN MOLECULAR BIOLOGY (John Wiley and Sons, N.Y. 1989). The transformants are plated on 1.5% agar plates (containing the 15 appropriate selection agent, ampicillin) to a density of about 150 transformants (colonies) per plate. These plates are screened using Nylon membranes according to routine methods for bacterial colony screening. See, Sambrook et al.

MOLECULAR CLONING: A LABORATORY MANUAL (Cold Spring Harbor, N.Y. 2nd ed. 1989); Ausubel et al., CURRENT PROTOCALS IN MOLECULAR 20 BIOLOGY (John Wiley and Sons, N.Y. 1989) or other techniques known to those of skill in the art.

Alternatively, two primers of 15-25 nucleotides derived from the 5' and 3' ends of a polynucleotide of Table 1 are synthesized and used to amplify the desired DNA by PCR using a E. faecalis genomic DNA prep as a template. PCR is carried out under routine conditions, for instance, in 25 tl of reaction mixture with 0.5 ug of the above DNA template. A convenient reaction mixture is 1.5-5 mM MgCI 2 0.01% gelatin, 20 pM each of dATP, dCTP, dGTP, dTTP, 25 pmol of each primer and 0.25 Unit of Taq polymerase. Thirty five cycles of PCR (denaturation at 94C for 1 min; annealing at 55 0 C for 1 min; elongation at 72 0 C for 1 min) are performed with a Perkin-Elmer Cetus automated thermal cycler. The amplified product is analyzed by agarose gel electrophoresis and the DNA. band with expected molecular weight is excised and purified. The PCR product is verified to be the selected sequence by subcloning and sequencing the DNA product.

Finally, overlapping oligos of the DNA sequences of Table 1 can be chemically synthesized and used to generate a nucleotide sequence of desired length using PCR methods known in the art.

Example Expression and Purification Enterococcal polypeptides in E. coli The bacterial expression vector pQE60 was used for bacterial expression of some of the polypeptide fragements used in the soft tissue and systemic infection models discussed below. (Q1AGEN, Inc., 9259 Eton Avenue, Chatsworth, CA, 91311). pQE60 encodes ampicillin antibiotic resistance ("Ampr") and contains a bacterial origin of replication an IPTG inducible promoter, a ribosome binding 15 site six codons encoding histidine residues that allow affinity purification using nickel-nitrilo-tri-acetic acid ("Ni-NTA") affinity resin (QIAGEN, Inc., supra) and suitable single restriction enzyme cleavage sites. These elements are arranged such that an inserted DNA fragment encoding a polypeptide expresses that polypeptide with the six His residues a "6 X His tag") covalently linked to the carboxyl terminus of that polypeptide.

The DNA sequence encoding the desired portion of a E. faecalis protein of the present invention was amplified from E. faecalis genomic DNA using PCR oligonucleotide primers which anneal to the 5' and 3' sequences coding for the portions of the E. faecalis polynucleotide shown in Table 1. Additional nucleotides containing restriction sites to facilitate cloning in the pQE60 vector are added to the and 3' sequences, respectively.

For cloning the mature protein, the 5' primer has a sequence containing an appropriate restriction site followed by nucleotides of the amino terminal coding sequence of the desired E. faecalis polynucleotide sequence in Table 1. One of ordinary skill in the art would appreciate that the point in the protein coding sequence where the 5' and 3' primers begin may be varied to amplify a DNA segment encoding any desired portion of the complete protein shorter or longer than the mature form.

The 3' primer has a sequence containing an appropriate restriction site followed by nucleotides complementary to the 3' end of the polypeptide coding sequence of Table 1, excluding a stop codon, with the coding sequence aligned with the restriction site so as to maintain its reading frame with that of the six His codons in the pQE60 vector.

The amplified E. faecalis DNA fragment and the vector pQE60 were digested with restriction enzymes which recognize the sites in the primers and the digested DNAs were then ligated together. The E. faecalis DNA was inserted into the restricted pQE60 vector in a manner which places the E.faecalis protein coding region downstream from the IPTG-inducible promoter and in-frame with an initiating AUG and the six histidine codons.

SThe ligation mixture was transformed into competent E. coli cells using 15 standard procedures such as those described by Sambrook et al., supra.. E. coli strain M 15/rep4, containing multiple copies of the plasmid pREP4, which expresses the lac repressor and confers kanamycin resistance was used in carrying out the illustrative example described herein. This strain, which was only one of many that are suitable for expressing a E.faecalis polypeptide, is available commercially 20 (QIAGEN, Inc., supra). Transformants were identified by their ability to grow on LB agar plates in the presence of ampicillin and kanamycin. Plasmid DNA was isolated from resistant colonies and the identity of the cloned DNA confirmed by restriction analysis, PCR and DNA sequencing.

Clones containing the desired constructs were grown overnight in liquid culture in LB media supplemented with both ampicillin (100 p.g/ml) and kanamycin (25 p.g/ml). The O/N culture was used to inoculate a large culture, at a dilution of approximately 1:25 to 1:250. The cells were grown to an optical density at 600 nm ("OD600") of between 0.4 and 0.6. Isopropyl-p-D-thiogalactopyranoside ("IPTG") was then added to a final concentration of 1 mM to induce transcription from the lac repressor sensitive promoter, by inactivating the lad repressor. Cells subsequently were incubated further for 3 to 4 hours. Cells then were harvested by centrifugation.

The cells were then stirred for 3-4 hours at 4 0 C in 6M guanidine-HCl, pH 8.

The cell debris was removed by centrifugation, and the supernatant containing the E.

faecalis polypeptide was loaded onto a nickel-nitrilo-tri-acetic acid ("Ni-NTA") affinity resin column (QIAGEN, Inc., supra). Proteins with a 6 x His tag bind to the Ni-NTA resin with high affinity were purified in a simple one-step procedure (for details see: The QlAexpressionist, 1995, QIAGEN, Inc., supra). Briefly the supernatant was loaded onto the column in 6 M guanidine-HC1, pH 8, the column was first washed with 10 volumes of 6 M guanidine-HCl, pH 8, then washed with volumes of 6 M guanidine-HCI pH 6, and finally the E. faecalis polypeptide was eluted with 6 M guanidine-HCI, pH The purified protein was then renatured by dialyzing it against 15s phosphate-buffered saline (PBS) or 50 mM Na-acetate, pH 6 buffer plus 200 mM NaCI. Alternatively, the protein could be successfully refolded while immobilized on the Ni-NTA column. The recommended conditions are as follows: renature using a linear 6M-1M urea gradient in 500 mM NaCI, 20% glycerol, 20 mM Tris/HC1 pH 7.4, containing protease inhibitors. The renaturation should be performed over a period of 20 1.5 hours or more. After renaturation the proteins can be eluted by the addition of 250 mM immidazole. Immidazole was removed by a final dialyzing step against PBS or 50 mM sodium acetate pH 6 buffer plus 200 mM NaCI. The purified protein was stored at 40 C or frozen at -80° C.

Some of the polypeptide of the present invention were prepared using a nondenaturing protein purification method. For these polypeptides, the cell pellet from each liter of culture was resuspended in 25 mls of Lysis Buffer A at 4 0 C (Lysis Buffer A 50 mM Na-phosphate, 300 mM NaCI, 10 mM 2-mercaptoethanol, Glycerol, pH 7.5 with 1 tablet of Complete EDTA-free protease inhibitor cocktail (Boehringer Mannheim #1873580) per 50 ml of buffer). Absorbance at 550 nm was approximately 10-20 O.D./ml. The suspension was then put through three freeze/thaw cycles from -70 0 C (using a ethanol-dry ice bath) up to room temperature.

The cells were lysed via sonication in short 10 sec bursts over 3 minutes at approximately 80W while kept on ice. The sonicated sample was then centrifuged at 15,000 RPM for 30 minutes at 4 0 C. The supernatant was passed through a column containing 1.0 ml of CL-4B resin to pre-clear the sample of any proteins that may bind to agarose non-specifically, and the flow-through fraction was collected.

The pre-cleared flow-through was applied to a nickel-nitrilo-tri-acetic acid ("Ni-NTA") affinity resin column (Quiagen, Inc., supra). Proteins with a 6 X His tag to bind to the Ni-NTA resin with high affinity and can be purified in a simple one-step procedure. Briefly, the supernatant was loaded onto the column in Lysis Buffer A at 4°C, the column was first washed with 10 volumes of Lysis Buffer A until the A280 of the eluate returns to the baseline. Then, the column was washed with 5 volumes of 40 mM Imidazole (92% Lysis Buffer A 8% Buffer B) (Buffer B 50 mM Na- 15 Phosphate, 300 mM NaCI, 10% Glycerol, 10 mM 2-mercaptoethanol, 500 mM Imidazole, pH of the final buffer should be The protein was eluted off of the column with a series of increasing Imidazole solutions made by adjusting the ratios of Lysis Buffer A to Buffer B. Three different concentrations were used: 3 volumes of mM Imidazole, 3 volumes of 150 mM Imidazole, 5 volumes of 500 mM 20 Imidazole. The fractions containing the purified protein were analyzed using 8 or 14% SDS-PAGE depending on the protein size. The purified protein was then dialyzed 2X against phosphate-buffered saline (PBS) in order to place it into an easily workable buffer. The purified protein was stored at 40 C or frozen at The following alternative method may be used to purify E. faecalis expressed in E coli when it is present in the form of inclusion bodies. Unless otherwise specified, all of the following steps are conducted at 4-10°C.

Upon completion of the production phase of the E. coli fermentation, the cell culture is cooled to 4-10°C and the cells are harvested by continuous centrifugation at 15,000 rpm (Heraeus Sepatech). On the basis of the expected yield of protein per unit weight of cell paste and the amount of purified protein required, an appropriate amount of cell paste, by weight, is suspended in a buffer solution containing 100 mM Tris, 50 mM EDTA, pH 7.4. The cells are dispersed to a homogeneous suspension using a high shear mixer.

The cells are then lysed by passing the solution through a microfluidizer (Microfuidics, Corp. or APV Gaulin, Inc.) twice at 4000-6000 psi. The homogenate is then mixed with NaCI solution to a final concentration of 0.5 M NaCI, followed by centrifugation at 7000 x g for 15 min. The resultant pellet is washed again using NaCI, 100 mM Tris, 50 mM EDTA, pH 7.4.

to The resulting washed inclusion bodies are solubilized with 1.5 M guanidine hydrochloride (GuHCI) for 2-4 hours. After 7000 x g centrifugation for 15 min., the pellet is discarded and the E.faecalis polypeptide-containing supernatant is incubated at 4 0 C overnight to allow further GuHCI extraction.

Following high speed centrifugation (30,000 x g) to remove insoluble particles, 15 the GuHCl solubilized protein is refolded by quickly mixing the GuHCI extract with volumes of buffer containing 50 mM sodium, pH 4.5, 150 mM NaCI, 2 mM EDTA by vigorous stirring. The refolded diluted protein solution is kept at 4 C without mixing for 12 hours prior to further purification steps.

To clarify the refolded E. faecalis polypeptide solution, a previously prepared tangential filtration unit equipped with 0.16 lm membrane filter with appropriate surface area Filtron), equilibrated with 40 mM sodium acetate, pH 6.0 is S: employed. The filtered sample is loaded onto a cation exchange resin Poros HS- Perseptive Biosystems). The column is washed with 40 mM sodium acetate, pH and eluted with 250 mM, 500 mM, 1000 mM, and 1500 mM NaCI in the same buffer, in a stepwise manner. The absorbance at 280 mm of the effluent is continuously monitored. Fractions are collected and further analyzed by SDS-PAGE.

Fractions containing the E.faecalis polypeptide are then pooled and mixed with 4 volumes of water. The diluted sample is then loaded onto a previously prepared set of tandem columns of strong anion (Poros HQ-50, Perseptive Biosystems) and weak anion (Poros CM-20, Perseptive Biosystems) exchange resins.

The columns are equilibrated with 40 mM sodium acetate, pH 6.0. Both columns are washed with 40 mM sodium acetate, pH 6.0, 200 mM NaC1. The CM-20 column is then eluted using a 10 column volume linear gradient ranging from 0.2 M NaCI, mM sodium acetate, pH 6.0 to 1.0 M NaCI, 50 mM sodium acetate, pH Fractions are collected under constant A 28 monitoring of the effluent. Fractions containing the E. faecalis polypeptide (determined, for instance, by 16% SDS-PAGE) are then pooled.

The resultant E. faecalis polypeptide exhibits greater than 95% purity after the above refolding and purification steps. No major contaminant bands are observed from Commassie blue stained 16% SDS-PAGE gel when 5 Ig of purified protein is loaded. The purified protein is also tested for endotoxin/LPS contamination, and typically the LPS content is less than 0.1 ng/ml according to LAL assays.

S 15 Example Alernative Expression and Purification Enterococcalpolypeptides in E.

coli Tthe vector pQE10 was alternatively used to clone and express some of the polypeptides of the present invention for use in the soft tissue and systemic infection models discussed below. The difference being such that an inserted DNA fragment 20 encoding a polypeptide expresses that polypeptide with the six His residues a "6 X His tag") covalently linked to the amino terminus of that polypeptide. The bacterial s* expression vector pQE10 (QIAGEN, Inc., 9259 Eton Avenue, Chatsworth, CA, 91311) was used in this example. The components of the pQEO1 plasmid are arranged such that the inserted DNA sequence encoding a polypeptide of the present invention expresses the polypeptide with the six His residues a "6 X His tag")) covalently linked to the amino terminus.

The DNA sequences encoding the desired portions of a polypeptide of Table 1 were amplified using PCR oligonucleotide primers from genomic E. faecalis DNA.

The PCR primers anneal to the nucleotide sequences encoding the desired amino acid sequence of a polypeptide of the present invention. Additional nucleotides containing restriction sites to facilitate cloning in the pQE 10 vector were added to the 5' and 3' primer sequences, respectively.

For cloning a polypeptide of the present invention, the 5' and 3' primers were selected to amplify their respective nucleotide coding sequences. One of ordinary skill in the art would appreciate that the point in the protein coding sequence where the and 3' primers begins may be varied to amplify a DNA segment encoding any desired portion of a polypeptide of the present invention. The 5' primer was designed so the coding sequence of the 6 X His tag is aligned with the restriction site so as to maintain its reading frame with that of E. faecalis polypeptide. The 3' was designed to include an stop codon. The amplified DNA fragment was then cloned, and the protein expressed, as described above for the pQE60 plasmid.

The DNA sequences encoding the amino acid sequences of Table 1 may also be cloned and expressed as fusion proteins by a protocol similar to that described *15 directly above, wherein the pET-32b(+) vector (Novagen, 601 Science Drive, Madison, WI 53711) is preferentially used in place ofpQElO.

.The above methods are not limited to the polypeptide fragements actually produced. The above method, like the methods below, can be used to produce either full length polypeptides or desired fragements therof.

Example Alternative Expression and Purification of Enterococcal polypeptides in E. coli The bacterial expression vector pQE60 is used for bacterial expression in this example (QIAGEN, Inc., 9259 Eton Avenue, Chatsworth, CA, 91311). However, in this example, the polypeptide coding sequence is inserted such that translation of the six His codons is prevented and, therefore, the polypeptide is produced with no 6 X His tag.

The DNA sequence encoding the desired portion of the E. faecalis amino acid sequence is amplified from an E. faecalis genomic DNA prep the deposited DNA clones using PCR oligonucleotide primers which anneal to the 5' and 3' nucleotide sequences corresponding to the desired portion of the E. faecalis polypeptides.

Additional nucleotides containing restriction sites to facilitate cloning in the vector are added to the 5' and 3' primer sequences.

For cloning a E. faecalis polypeptides of the present invention, 5' and 3' primers are selected to amplify their respective nucleotide coding sequences. One of ordinary skill in the art would appreciate that the point in the protein coding sequence where the 5' and 3' primers begin may be varied to amplify a DNA segment encoding any desired portion of a polypeptide of the present invention. The 3' and 5' primers contain appropriate restriction sites followed by nucleotides complementary to the and 3' ends of the coding sequence respectively. The 3' primer is additionally designed to include an in-frame stop codon.

The amplified E. faecalis DNA fragments and the vector pQE60 are digested with restriction enzymes recognizing the sites in the primers and the digested DNAs 15 are then ligated together. Insertion of the E.faecalis DNA into the restricted vector places the E. faecalis protein coding region including its associated stop codon downstream from the IPTG-inducible promoter and in-frame with an initiating AUG.

The associated stop codon prevents translation of the six histidine codons downstream of the insertion point.

The ligation mixture is transformed into competent E. coli cells using standard procedures such as those described by Sambrook et al. E. coli strain M15/rep4, containing multiple copies of the plasmid pREP4, which expresses the lac repressor *o and confers kanamycin resistance is used in carrying out the illustrative example described herein. This strain, which is only one of many that are suitable for expressing E. faecalis polypeptide, is available commercially (QIAGEN, Inc., supra).

Transformants are identified by their ability to grow on LB plates in the presence of ampicillin and kanamycin. Plasmid DNA is isolated from resistant colonies and the identity of the cloned DNA confirmed by restriction analysis, PCR and DNA sequencing.

Clones containing the desired constructs are grown overnight in liquid culture in LB media supplemented with both ampicillin (100 p.g/ml) and kanamycin The O/N culture is used to inoculate a large culture, at a dilution of approximately 1:25 to 1:250. The cells are grown to an optical density at 600 nm ("OD600") of between 0.4 and 0.6. isopropyl-b-D-thiogalactopyranoside ("IPTG") is then added to a final concentration of 1 mM to induce transcription from the lac repressor sensitive promoter, by inactivating the lad repressor. Cells subsequently are incubated further for 3 to 4 hours. Cells then are harvested by centrifugation.

To purify the E. faecalis polypeptide, the cells are then stirred for 3-4 hours at 4 0 C in 6M guanidine-HC1, pH 8. The cell debris is removed by centrifugation, and the supernatant containing the E. faecalis polypeptide is dialyzed against 50 mM Naacetate buffer pH 6, supplemented with 200 mM NaCI. Alternatively, the protein can be successfully refolded by dialyzing it against 500 mM NaCI, 20% glycerol, mM Tris/HCl pH 7.4, containing protease inhibitors. After renaturation the protein 15 can be purified by ion exchange, hydrophobic interaction and size exclusion chromatography. Alternatively, an affinity chromatography step such as an antibody column can be used to obtain pure E. faecalis polypeptide. The purified protein is stored at 40 C or frozen at -80° C.

The following alternative method may be used to purify E. faecalis S 20 polypeptides expressed in E coli when it is present in the form of inclusion bodies.

Unless otherwise specified, all of the following steps are conducted at 4-10°C.

S. Upon completion of the production phase of the E. coli fermentation, the cell culture is cooled to 4-10 0 C and the cells are harvested by continuous centrifugation at 15,000 rpm (Heraeus Sepatech). On the basis of the expected yield of protein per unit weight of cell paste and the amount of purified protein required, an appropriate amount of cell paste, by weight, is suspended in a buffer solution containing 100 mM Tris, 50 mM EDTA, pH 7.4. The cells are dispersed to a homogeneous suspension using a high shear mixer.

The cells ware then lysed by passing the solution through a microfluidizer (Microfuidics, Corp. or APV Gaulin, Inc.) twice at 4000-6000 psi. The homogenate is then mixed with NaCI solution to a final concentration of 0.5 M NaCI, followed by centrifugation at 7000 x g for 15 min. The resultant pellet is washed again using NaCI, 100 mMl Tris, 50 mM EDTA, pH 7.4.

The resulting washed inclusion bodies are solubilized with 1.5 M guanidine hydrochloride (GuHCI) for 2-4 hours. After 7000 x g centrifugation for 15 min., the pellet is discarded and the E.faecalis polypeptide-containing supernatant is incubated at 4 0 C overnight to allow further GuHCI extraction.

Following high speed centrifugation (30,000 x g) to remove insoluble particles, to the GuHCI solubilized protein is refolded by quickly mixing the GuHCI extract with volumes of buffer containing 50 mM sodium, pH 4.5, 150 mM NaCI, 2 mM EDTA by vigorous stirring. The refolded diluted protein solution is kept at 4°C without mixing for 12 hours prior to further purification steps.

To clarify the refolded E. faecalis polypeptide solution, a previously prepared S^ 15 tangential filtration unit equipped with 0.16 prm membrane filter with appropriate surface area Filtron), equilibrated with 40 mM sodium acetate, pH 6.0 is employed. The filtered sample is loaded onto a cation exchange resin Poros HS- Perseptive Biosystems). The column is washed with 40 mM sodium acetate, pH 6.0 and eluted with 250 mM, 500 mM, 1000 mM, and 1500 mM NaCI in the same buffer, in a stepwise manner. The absorbance at 280 mm of the effluent is continuously monitored. Fractions are collected and further analyzed by SDS-PAGE.

Fractions containing the E.faecalis polypeptide are then pooled and mixed with 4 volumes of water. The diluted sample is then loaded onto a previously prepared set of tandem columns of strong anion (Poros HQ-50, Perseptive Biosystems) and weak anion (Poros CM-20, Perseptive Biosystems) exchange resins.

The columns are equilibrated with 40 mM sodium acetate, pH 6.0. Both columns are washed with 40 mM sodium acetate, pH 6.0, 200 mM NaCI. The CM-20 column is then eluted using a 10 column volume linear gradient ranging from 0.2 M NaCI, mM sodium acetate, pH 6.0 to 1.0 M NaCI, 50 mM sodium acetate, pH Fractions are collected under constant Azso monitoring of the effluent. Fractions containing the E. faecalis polypeptide (determined, for instance, by 16% SDS-PAGE) are then pooled.

The resultant E. faecalis polypeptide exhibits greater than 95% purity after the above refolding and purification steps. No major contaminant bands are observed from Commassie blue stained 16% SDS-PAGE gel when 5 p.g of purified protein is loaded. The purified protein is also tested for endotoxin/LPS contamination, and typically the LPS content is less than 0.1 ng/ml according to LAL assays.

o1 Example Cloning and Expression of E. faecalis in Other Bacteria E. faecalis polypeptides can also be produced in: E.faecalis using the methods of S. Skinner et al., (1988) Mol. Microbiol. 2:289-297 or J. 1. Moreno (1996) Protein Expr. Purif. 8(3):332-340; Lactobacillus using the methods of C. Rush et al., 1997 Appl. Microbiol. Biotechnol. 47(5):537-542; or in Bacillus subtilis using the methods 15 Chang et al., U.S. Patent No. 4,952,508.

Example 3: Cloning and Expression in COS Cells A E. faecalis expression plasmid is made by cloning a portion of the DNA encoding a E. faecalis polypeptide into the expression vector pDNAI/Amp or pDNAIIl (which can be obtained from Invitrogen, Inc.). The expression vector pDNAI/amp contains: an E. coli origin of replication effective for propagation in E. coli and other prokaryotic cells; an ampicillin resistance gene for selection of plasmid-containing prokaryotic cells; an SV40 origin of replication for propagation in eukaryotic cells; a CMV promoter, a polylinker, an SV40 intron; several codons encoding a hemagglutinin fragment an "HA" tag to facilitate purification) followed by a termination codon and polyadenylation signal arranged so that a DNA can be conveniently placed under expression control of the CMV promoter and operably linked to the SV40 intron and the polyadenylation signal by means of restriction sites in the polylinker. The HA tag corresponds to an epitope derived from the influenza hemagglutinin protein described by Wilson et al. 1984 Cell 37:767.

The fusion of the HA tag to the target protein allows easy detection and recovery of the rccombinant protein with an antibody that recognizes the HA epitope. pDNAIll contains, in addition, the selectable neomycin marker.

A DNA fragment encoding a E. faecalis polypeptide is cloned into the polylinker region of the vector so that recombinant protein expression is directed by the CMV promoter. The plasmid construction strategy is as follows. The DNA from a E. faecalis genomic DNA prep is amplified using primers that contain convenient restriction sites, much as described above for construction of vectors for expression of E. faecalis in E. coli. The 5' primer contains a Kozak sequence, an AUG start codon, and nucleotides of the 5' coding region of the E. faecalis polypeptide. The 3' primer, contains nucleotides complementary to the 3' coding sequence of the E. faecalis DNA, a stop codon, and a convenient restriction site.

The PCR amplified DNA fragment and the vector, pDNAI/Amp, are digested with appropriate restriction enzymes and then ligated. The ligation mixture is transformed into an appropriate E. coli strain such as SURET (Stratagene Cloning Systems, La Jolla, CA 92037), and the transformed culture is plated on ampicillin media plates which then are incubated to allow growth of ampicillin resistant colonies.

Plasmid DNA is isolated from resistant colonies and examined by restriction analysis or other means for the presence of the fragment encoding the E. faecalis polypeptide For expression of a recombinant E. faecalis polypeptide, COS cells are transfected with an expression vector, as described above, using DEAE-dextran, as described, for instance, by Sambrook et al. (supra). Cells are incubated under conditions for expression of E. faecalis by the vector.

Expression of the E. faecalis-HA fusion protein is detected by radiolabeling and immunoprecipitation, using methods described in, for example Harlow et al., supra.. To this end, two days after transfection, the cells are labeled by incubation in media containing "S-cysteine for 8 hours. The cells and the media are collected, and the cells are washed and the lysed with detergent-containing RIPA buffer: 150 mM NaCI, 1% NP-40, 0.1% SDS, 1% NP-40, 0.5% DOC, 50 mM TRIS, pH 7.5, as described by Wilson et al. (supra Proteins are precipitated from the cell lysate and from the culture media using an HA-specific monoclonal antibody. The precipitated proteins then are analyzed by SDS-PAGE and autoradiography. An expression product of the expected size is seen in the cell lysate, which is not seen in negative controls.

Example 4. Cloning and Expression in CHO Cells The vector pC4 is used for the expression of E. faecalis polypeptide in this example. Plasmid pC 4 is a derivative of the plasmid pSV2-dhfr (ATCC Accession No. 37146). The plasmid contains the mouse DHFR gene under control of the early promoter. Chinese hamster ovary cells or other cells lacking dihydrofolate activity that are transfected with these plasmids can be selected by growing the cells in a selective medium (alpha minus MEM, Life Technologies) supplemented with the 15 chemotherapeutic agent methotrexate. The amplification of the DHFR genes in cells resistant to methotrexate (MTX) has been well documented. See, Alt et al., 1978, J. Biol. Chem. 253:1357-1370; Hamlin et al., 1990, Biochem. et Biophys. Acta, 1097:107-143; Page et al., 1991, Biotechnology 9:64-68. Cells grown in increasing concentrations of MTX develop resistance to the drug by overproducing the target enzyme, DHFR, as a result of amplification of the DHFR gene. If a second gene is linked to the DHFR gene, it is usually co-amplified and over-expressed. It is known in the art that this approach may be used to develop cell lines carrying more than S1,000 copies of the amplified gene(s). Subsequently, when the methotrexate is withdrawn, cell lines are obtained which contain the amplified gene integrated into one or more chromosome(s) of the host cell.

Plasmid pC4 contains the strong promoter of the long terminal repeat (LTR) of the Rouse Sarcoma Virus, for expressing a polypeptide of interest, Cullen, et al.

(1985) Mol. Cell. Biol. 5:438-447; plus a fragment isolated from the enhancer of the immediate early gene of human cytomegalovirus (CMV), Boshart, et al., 1985, Cell 41:521-530. Downstream of the promoter are the following single restriction enzyme cleavage sites that allow the integration of the genes: Barn HI, Xba 1, and Asp 718.

Behind these cloning sites the plasmid contains the 3' intron and polyadenylation site of the rat preproinsulin gene. Other high efficiency promoters can also be used for the expression, the human 1-actin promoter, the SV40 early or late promoters or the long terminal repeats from other retroviruses, HIV and HTLVI. Clontech's Tet- Off and Tet-On gene expression systems and similar systems can be used to express the E. faecalis polypeptide in a regulated way in mammalian cells (Gossen et al., 1992, Proc. Natl. Acad. Sci. USA 89:5547-5551. For the polyadenylation of the mRNA other signals, from the human growth hormone or globin genes can be used as well. Stable cell lines carrying a gene of interest integrated into the chromosomes can also be selected upon co-transfection with a selectable marker such as gpt, G418 or hygromycin. It is advantageous to use more than one selectable marker in the beginning, G418 plus methotrexate.

15 The plasmid pC4 is digested with the restriction enzymes and then dephosphorylated using calf intestinal phosphates by procedures known in the art.

The vector is then isolated from a 1% agarose gel. The DNA sequence encoding the E.

faecalis polypeptide is amplified using PCR oligonucleotide primers corresponding to the 5' and 3' sequences of the desired portion of the gene. A 5' primer containing a 20 restriction site, a Kozak sequence, an AUG start codon, and nucleotides of the coding region of the E.faecalis polypeptide is synthesized and used. A 3' primer, containing a restriction site, stop codon, and nucleotides complementary to the 3' coding sequence of the E. faecalis polypeptides is synthesized and used. The amplified fragment is digested with the restriction endonucleases and then purified again on a 1% agarose gel. The isolated fragment and the dephosphorylated vector are then ligated with T4 DNA ligase. E. coli HB 101 or XL-1 Blue cells are then transformed and bacteria are identified that contain the fragment inserted into plasmid pC4 using, for instance, restriction enzyme analysis.

Chinese hamster ovary cells lacking an active DHFR gene are used for transfection. Five p.g of the expression plasmid pC4 is cotransfected with 0.5 j.g of the plasmid pSVneo using a lipid-mediated transfection agent such as Lipofectin T or LipofectAMINE.

T

r (LifeTechnologies Gaithersburg, MD). The plasmid pSV2-neo contains a dominant selectable marker, the neo gene from Tn5 encoding an enzyme that confers resistance to a group of antibiotics including G418. The cells are seeded in alpha minus MEM supplemented with 1 mg/ml G41 8. After 2 days, the cells are trypsinized and seeded in hybridoma cloning plates (Greiner, Germany) in alpha minus MEM supplemented with 10, 25, or 50 ng/ml of methotrexate plus 1 mg/ml G418. After about 10-14 days single clones are trypsinized and then seeded in 6-well petri dishes or 10 ml flasks using different concentrations of methotrexate (50 nM, 100 nM, 200 nM, 400 nM, 800 nM). Clones growing at the highest concentrations of methotrexate are then transferred to new 6-well plates containing even higher concentrations of methotrexate (1 pM, 2 .tM, 5 p.M, 10 mM, 20 mM). The same procedure is repeated until clones are obtained which grow at a concentration of 15 100-200 ptM. Expression of the desired gene product is analyzed, for instance, by SDS-PAGE and Western blot or by reversed phase HPLC analysis.

Example 5. Quantitative Murine Soft Tissue Infection Model for E. faecalis Compositions of the present invention, including polypeptides and peptides, are assayed for their ability to function as vaccines or to enhance/stimulate an immune response to a bacterial species E. faecalis) using the following quantitative murine soft tissue infection model. Mice NIH Swiss female mice, approximately 7 weeks old) are first treated with a biologically protective effective amount, or immune enhancing/stimulating effective amount of a composition of the present invention using methods known in the art, such as those discussed above. See,e.g., Harlow et al., ANTIBODIES: A LABORATORY MANUAL, (Cold Spring Harbor Laboratory Press, 2nd ed. 1988). An example of an appropriate starting dose is per animal.

The desired bacterial species used to challenge the mice, such as E. faecalis, is grown as an overnight culture. The culture is diluted to a concentration of 5 X 108 cfu/ml, in an appropriate media, mixed well, serially diluted, and titered. The desired doses are further diliuted 1:2 with sterilized Cytodex 3 microcarrier beads preswollen in sterile PBS (3g/100ml). Mice are anesthetize briefly until docile, but still mobile and injected with 0.2 ml of the Cytodex 3 bead/bacterial mixture into each animal subcutaneously in the inguinal region. After four days, counting the day of injection as day one, mice are sacrificed and the contents ofthe abscess is excised and placed in a 15 ml conical tube containing 1.0ml of sterile PBS. The contents of the abscess is then enzymatically treated and plated as follows.

The abscess is first disrupted by vortexing with sterilized glass beads placed in the tubes. 3.0mls of prepared enzyme mixture (1.0ml Collagenase D (4.0 mg/ml), 1.0ml Trypsin (6.0 mg/ml) and 8.0 mis PBS) is then added to each tube followed by a min. incubation at 37C. The solution is then centrifuged and the supernatant drawn off. 0.5 ml dH20 is then added and the tubes are vortexed and then incubated for 10 min. at room temperature. 0.5 ml media is then added and samples are serially i diluted and plated onto agar plates, and grown overnight at 37C. Plates with distinct and separate colonies are then counted, compared to positive and negative control samples, and quantified. The method can be used to identify composition and 20 determine appropriate and effective doses for humans and other animals by comparing the effective doses of compositions of the present invention with compositions known in the art to be effective in both mice and humans. Doses for the effective treatment of humans and other animals, using compositions of the present invention, are extrapolated using the data from the above experiments of mice. It is appreciated that further studies in humans and other animals may be needed to determine the most effective doses using methods of clinical practice known in the art.

Example 6: Murine Systemic Neutropenic Model for E. faecalis Infection Compositions of the present invention, including polypeptides and peptides, are assayed for their ability to function as vaccines or to enhance/stimulate an immune response to a bacterial species E.faecalis) using the following qualitative murine systemic neutropenic model. Mice N1H Swiss female mice, approximately 7 weeks old) are first treated with a biologically protective effective amount, or immune enhancing/stimulating effective amount of a composition of the present invention using methods known in the art, such as those discussed above. See,e.g., Harlow et al., ANTIBODIES: A LABORATORY IMANUAL, (Cold Spring Harbor Laboratory Press, 2nd ed. 1988). An example of an appropriate starting dose is 20ug per animal.

in Mice are then injected with 250 300 mg/kg cyclophosphamide intraperitonially.

Counting the day of C.P. injection as day one, the mice are left untreated for 5 days to begin recovery of PMNL'S.

The desired bacterial species used to challenge the mice, such as E. faecalis, is grown as an overnight culture. The culture is diluted to a concentration of 5 X 108 S 5 cfu/ml, in an appropriate media, mixed well, serially diluted, and titered. The desired doses are further diliuted 1:2 in 4% Brewer's yeast in media.

Mice are injected with the bacteria/brewer's yeast challenge intraperitonially. The Brewer's yeast solution alone is used as a control. The mice are then monitered twice daily for the first week following challenge, and once a day for the next week to ascertain morbidity and mortality. Mice remaining at the end of the experiment are sacrificed. The method can be used to identify compositions and determine appropriate and effective doses for humans and other animals by comparing the effective doses of compositions of the present invention with compositions known in the art to be effective in both mice and humans. Doses for the effective treatment of humans and other animals, using compositions of the present invention, are extrapolated using the data from the above experiments of mice. It is appreciated that further studies in humans and other animals may be needed to determine the most effective doses using methods of clinical practice known in the art.

The disclosure of all publications (including patents, patent applications, journal articles, laboratory manuals, books, or other documents) cited herein are hereby incorporated by reference in their entireties.

The present invention is not to be limited in scope by the specific embodiments described herein, which are intended as single illustrations of individual aspects of the invention. Functionally equivalent methods and components are within the scope of the invention, in addition to those shown and described herein and will become apparant to those skilled in the art from the foregoing description and accompanying drawings. Such modifications are intended to fall within the scope of the appended claims.

Throughout the specification, unless the context requires otherwise, the word "comprise" or variations such as "comprises" or "comprising", will be understood to imply the inclusion of a stated integer or group of integers but not the exclusion of any other integer or group of integers.

e 79 TABLE 1. Nucleotide and Amiho Acid Seqeuences of E. faecalis Genes.

EFOO1-1 (SEQ ID NQ:l)

TGAAAGAATA

AAAAAAATGA

TTGGGGGCVIT

AAGGAAGATA

ACAAAATTAA

CAATCTGCTT

TTACCAACAA

TTAGTGGACT

ATTCGTGAAG

AATAAATCGA

GTACCGAAAA

AAAGAAGTCG

AACAAAGGCG

GTGGACTATT

TATTIATCTG

GCTGGTT'rTG CCTrGAAAAAA

GAACAACGGA

TACTGGGCAC

TACAAAAATT

TTCGCTATCC

GAAAGTATTT

CAATTTGAAC

TTGCCAGAAC

AG 1TCAAAAC

GTGGTAACGG

CCACAATCAC

CGAAAGACT

ACCCTGATTT

TTACCCA.AC

TAAA.ACCATA

TATTGTTAGA

CAGAAATGTT

CATTAGAGGA

TTGGTGCTGG

TTGATTTTAA

ACCGTGATGG

GCCCATTTGC

TTCAAAAAGA

TCAACTTACA

CAGCGGCATT

AACAAACAGG

CTAAGACAAC

CAGTAGAAGA

TTGC-TTCATC

AAGCATGGAA

GTGGCGAGCA

TCTAGCAACA

TAATGGGGCC

TTrCTGGCAT

CATGAAAGAA

ACAAGCCAAA

GTACCCAGGC

TATGGATGAT

CGGCGCCAAA

ATTCTATAAT

ATTAAA.AGAA

rTIGACTCG

TAAAGACTTA

TATCGAAGCA

AAACAAAAAG

TGCTGAAGCT

ACAAGGAACA

TGAATTCATG

TTATATGCCA

CAAAGTACCT

AAATGCTGAT

AAATAAAGAC

CCAATAA

AATTOTI'TTA

ACAGTGTTAG

AAAGAATCAA

GCAATGAATG

AATCCAAAAA

ATCAATTCGA

TGGTTATGGA

GACACAATCG

ATCGACGGCA

G6TGATTGT

GCTTCTAAAA

TTAAATAACT

GATTAACAA

GGTTACTTCC

GTAGCAATGT

GGTGGCTATG

GATATTTATA

AA.ATTCTTAG

ATTFAGAAT

GCACAACTTG

TCAGCCTATA

ACGAGAAA-AT

TAAATTTTTT

CAACCGCAGC

ACGATATTGT

GGGTTCAAGA

TTAAAGTGGA

CTTTAACTTC

ATGCTGCACA

GCTGGAAAGA

AACAATACGG

TGAAAGAATA

CAATTTACrGA

ATTACGPAAT

GCAAAGATTC

GCACAGCT-GG

TI'GTCGGT2AG

AATACGGTGT

TGITCGATAG

CTACTCCTGA

CTGTTTTACA

AAAACGCAGT

ATGAAATGCG

TATTGAAAGA

TAAGGGAGAG

TATTCGCA

GAAAGAAGTG

AGAAGCGTTA

ATTACAAAAT

ACCAAAAGAT

AGATGAAATG

TrGCAGAGCCA

CATTCCATTT

TGGTGTTGAA

AAAATCCAAC

TGGAATGAA.A

ACAAGAAGTC

TTCAGATAAA

TATTGCTGGT

TGCACCACGT

TGCTACGCCA

TTCACAATTG

CAGTGATGAG

AAAAGATTTA

GACAATTATG

TGCAACATCA

EF001-2 (SEQ ID NO:2) MFXTLATT VLATAAIFALj GACGNGNGAK ESNDIVKEVK, a.

EDTTITFWHA

PTITQAYPGW

KSTEMLFYNA

KGVDFNKDLD

GFVQKDAE-AG

WAQQTG;YMP I S IFASSNKDT M4NGVQEEALT LWNAAQDE4L

DLLKEYGVEV

LTSKDSQEVV

GYEYGVAPRP

LESVLHSDEY

RKLLKDATSQ

KLTKDFI<EN

VDLKPYMDDD

PKTLEELKEA

DYYRDGIEAG

EKINLQQGTD

KNSKTTKVPA

FEQAWNQ

PK IKVELQNQ

TIGWKCDAEPI

SKTIYEKSNfl

YFRTAGSOKY

IYMFDSATPE

QLEN'AVKDLF

SAYPDLQAKI

REVLLDGAKI

EVVGAGFDSL

LSGPFANXKV

QRTAAFEFMfl

AIPVEENADS

NSTLTSPKDL

DGKQYGIPFN

NNY"YAIGMKN

AMFVGS IAGA

FLATPOSQLY

AYNEMRTIME

EF001-3 (SEQ ID NO:3) TT GTGGTAACGG TAATGGGGCC AAAGAATCAA ACGATATTGT GAAAG-AGTG

AAGGAAGATA

ACAAAMTTAA

CAATCTGCTT

TTACCAACAA

TTAGTGGACT

ATTCGTGAAG

AATAAATCGA

GTACCGAAAA

AAAGAAGTCG

AACAAAGGCG

GTGGACTATT

TArI-rATCTG

CGACAATCAC

CGAAAGACTT

ACCCTGATTT

TTACGCAAGC

TAAAACCATA

TATTGTTAGA

CAGAAATGTT

CATTAGAGGA

TTGGTGCTGG

TTGATTTTAA

ACCGTGATGG

CCCCATTTrGC

TTTCTGGCAT

CATGAAAGAA

ACMA.GCCAAA

GTACCCAGGC

TATGGATGAT

CGGCGCCAAA

ATTCTATAAT

ATTAAAAGAA

TrTTGACTCG

TAAAGACTTA

TATCGAAGCA

AAACAAAAAG

GCAATGAAG

AATCCAAAAA

ATCAATTCGA

TGGTITATIGGA

GACACAATCG

ATCGACGGCA

GCTGATTTGT

GCTTCTAAAA

TTAAATAACT

GATTTAZACAA

GGTTACITCC

GTAGCAATGT

GGGTTCA.AGA

TTAAAGTGGA

CTTAACTTC

ATGCTGCACA

GCTGGAAAGA

AACAATACGG

TGAAAGAATA

cAATTTACGA

ATTACCCAAT

GCAAAGATTC

GCACAGCTGG

TTGTCGGTAG

AGAAGCGTT1A

ATTACAAAAT

ACCAAAAGAT

AGATGAAATG

TGC-AGAGCCA

CATTCCATT

TGGTGTTGAA

AAAATCCAAC

TGGAATGAAA

ACAAGAAGTC

TTICAGATAAA

TATTGCTGGT

TABLE 1. Nucleofide and Amino Acid Seqeuences of E.f[aecalis Genes.

GCTGGTTTG

CCTGAAAAAA

GAACAACGGA

TACTCGGCAC

TACAAAAATT

TTCGCTATCC

GAAAGTATTT

CAATTTGAAC

TTCAAAA.AGA

TCAACTTACA

CAGCGGCATT

AACAAACAGG

CTAAGACAAC

CAGTAGAAGA

TTGCTTCATC

AAGCATGGA.A

TGCTGAAGCT

ACAAGGAACA

TGAATTICATG

TTATATGCCA

CAAAGTACCT

AAATGCTGAT

AAATAAAGAC

CCAA

GGTGGCTATC

GATATTATA

AAATTCTTAG

ATT~rAGA-AT

GCACAACI'

TCAGCCTATA

ACCAGAAAAT

AATACGGTrGT

TGOTTCGATAG

CTACTCCTGA

CTG'T=TACA

AAAACGCAGT

ATGAAAT'GCG

TATTGAAAGA

TGCACCACCT

TGCTACGCCA

TrCACAl-rG

CACTGATGAG

AAAAGATrA

GACAATI'ATG

TGCAACATCA

EF001-4 (SEQ ID NO:4) CGNGNGA< ESNDIVKEVK EDTTITFWHA MNGVQEE-ALT PTITQAYPGW LWNAAQDEML KSTZEMLFYNA DLLKEYGVEV KGVDFNKDLD LTSKDSQEVV GFVQKDAEAG GYEYGVAPRP WAQQTGYMP I LESVLHSDEY SIFASSNXDT RKLLKDATSQ FF002-1 (SEQ ID KLTKDFrMEN

VDLKPYMDDD

PKTLEELKEA

DYYREXMEAG

EKINLQQGTD

KNSKTI'KVPA

FEQAWNQ

PKIKVFELQNQ

TIGWKDAEPI

SKTIYEKSNK

YFRTAGSDKY

IYMFDSATPE

QLENAVYKDLF

SAYPDLQAKI

REVLLDGAKI

EVVGAGFDSL

LSGPFANKKV

QRTAAFEFMK

Al PVEENADS

NSTLTSPKDL

DGKQYGIPFN

NNYYA IGbM(N AMFVGS IAGA

FLATPDSQLY

AYNEMRTIME

S S

S

5 p

TAAATAGCGG

TTAGCAGTGG

GAAAAGAGTG

CCAGAATTTG

CCGGTGGACA

GATACGACGG

AATCAATTGG

AGTTACGAGA

TGGGTATTGT

TTAACTI'GGG

TATGGTG CCT

AATGCCAATT

AGAATGCAAA

TATCAATCAC

GGGACTTI'AT

ATACCACAAC

AATAAAAACA

GAAGGTGCAA

GATAAAATCT

AGGTAGTACA

CGGCACTAAC

AAGATGGCAA

AGAAATTA T

TTGCTTCAGA

ATATTTTAAC

T'GGATTTAAC

TGTATGAAAT

ATTACAATAA

ATGAATATGA

ATCAACATAC

TGATTGAACC

AAGATCAATC

AATI!TGAAAA

TAACAAACAT

AAGAAAAGG

GTAAAAAACA

AACTT'ITAGC

AC'ITTGCAAG

AATGAAATI"I

TTTAACAGCA

AACAAAATTA

CAGAGCTTTT

TGATTATGAC

CATGAA-AAAC

CGATCACG'11

CGATGGTAAA

AAAAATGTTT

AGCGTTAGCG

TTGGCGCTCA

AAAATACAAT

ACAAATGGAT

TCAAAAGCG

TGATGATGGC

CAAAGCAACT

AAAAGCTGCT

AGAAGTAGGG

AAAAGGAATG

TGGAAAA-AAG

TGTGGTGGTT

ACAGTAACTA

GAAGCGGAAA

ACAAAAGTAA

TTACIFICAT

AAAGATTTAG

ACCTATGCTC

GATGAAGCCG

AAAAAATTAT

ACCGT1'CAAG

TATATGGAAA

TTTGGAACAG

GCGATGATGT

AAAACAAATG

ACCTTI'1'GCT

CAAAAATTCT

GTGGTTCCTT

CCTI'CAGACG

GCTTAACAGC

CAAGTGAAAA

CT-TGGAATTA

ATCCTGATAT

CAACGATGCT

'ATTCTAATTA

ATATCGAACC

AGCCTTACCG

GAATTGCCTA

CTAAACCAGA

CGATI'GCTGC

CTrATTATGA

CAAAATCAAC

ACATGGGTAG

TCGAATGGGG

CACCGACAAC

TAGACTTTG-C

C'FrATAAAAC

AGTCTCACAA

GGCAGCGCTG

GAAACCAACT

TGACACGACC

CACTATTGAA

'PrCATCAGGA

CGCGCTACGC

TGCCAAAGCA

TACAGATTC

TCCCCATAAC

AGAACAAGTA

TGCTCAAAAC

TCGCGCATTG

AAAAGTAACG

CTGGTACATG

GATTGCCGAA

TTTTGCAATT

TICAGGTAAA

AGATGAAATT

AAAGCC'ITTA

ACCCAGATAC AATTAATTTA G EF002-2 (SEQ ID NO:6) MXFW KXGLTAAALL AVAAVTLTAC GGSSEKKATE KSEDGKTXLT VMITNYDIfP EFEKLFRAFE AENPDITIEP VDIASDDYDT KVTI'MLSSGD TTDI LTMKJNL LSYSNYALRN QLVDLTDHVI( DLDIEPAKAS YEMYEIDGKT YAQPYRTDFW VLYYNKKMFD EAGIAYPDNL TWDEYE-ALAX KLSKPEEQVY GAYQHWRST VQAIAAAQNN ANLIEPRYNY METYYDRALR MQKDQSQMDF GTAKSTKVTY QSQFENSKAA MMYMGSWYMG TLLTNIDDGK TNVEWGIAEI PQQEKGKATT FGSPTSFAIN KNSKKQKAAQ KFLDFASGKE GAKLLAEVGV VPSYKTDEID 81 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.f[aecalis Genes.

KIYFARKGMP SDESHKKPLT QIQLI FQO02-3 (SEQ ID NO:7) A TGTGGTGGTT CAAGTGAAAA GAAACCAAC GAAAAGAGTC- A.AGATG.GCAA AACAAAA LTA

CCAGAATI'G

CCGGTGGACA

GATACGACGG

AATCAATTGG

AGTTACGAGA

TGGCCTATTGT

TTAACTTGGG

TATGGTG-CCT

AATGCCAArI'

AGAATGCAAA

TATCAATCAC

GGGACTTTAT

ATACCACAAC

AATAAAAACA

CAAGGTGCAA

GATAAAATCT

ACCCAGATAC

AGAAATTATT

TTGCTTCAGA

ATA TITTAAC

TGGATTTAAC

TGTATGAAAT

ATTACAATA

ATGAATATGA

ATCAACATAC

TGATTGAACC

AAGATCAATC

AATTTGAAAA

TAACAAACAT

AAGAAAAAGG

GTAAAAAACA

AACTITTAC

ACTTI'GCAAG

AATTAATT

CAGAGCTTTT

TGATTATGAC

CATGAAAAAC

CGATCACGTT

CGATGGTAAA

AAAAATGTTT

AGCGTTAGCG

TTGGCGCTCA

AAAATACAAT

ACAAATGGAT

TTCAAAAGCG

TGATGATGC

CAAAGCAACT

AAAAGCTGCT

AGAAGTAGGG

AAAAGGAATG

T

ACAGTAACTA

GAAGCGGAAA

ACAAALAGTAA

TTACTTTCAT

AALAGATTAG

ACCTATGCTC

GATGAAGCCG

AAAAAATTAT

ACCGTTCAAG

TATATGGAAA

TTTGGAACAG

GCGATGATGT

AA.AACAAATG

ACCTTTGGcT

CAAAAATTCT

GTGGTTCCTT

CCTTCAGACG

cTI'GGAATTA

ATCCTGATAT

CAACGATGCT

ATTCTAATTA

ATATCGAACC

AGC CTTACCG

GAATTGCCTA

CTA-A.ACCAGA

CGATT GCTGC CTTAkTTATGA

CAAAATCAAC

ACATGGGTAG

TCGAATGGGG

CACCGACAAG

TAGACTTTGC

C'TATAAAAC

IAGTCTCACAP

TGACACGACC

CACTATTGAA

TTCATCAGGA

CGCGCTACGC

TGCCAAAGCA

TACAGATTTC

TCCCGATAAC

AGAACAAGTA

TGCTCAAAAC

TCGCGCATTG

AAAAGTAACG

CTGGTACATG

GATTGCCGAA

TTTGCAATT

TTCAGGTAAA

AGATGAAATT

AAAGCCTTTA

9* 9* S S EF002-4 (SEQ ID N0:8) C GGSSEKKATE KSEDGKTKLT VTTWNYDTTP EFEKLFRAFE AENPDITIEP VDIASDDYDT KVTTMLSSGD TTDILTMKNL QLVDLTDHVK OLDIEPAKAS YEMYEIDGKT YAQPYRTDFW VLYYNKK<MFD TWDEYEALAK KLSKPEEQVY GAYQHTWRST VQAIAAAQNN ANL IEPYYNY MQKDQSQMDF GTAKSTKVTY QSQFENSKAA MJ4YMGSWYMG TLLTN~IDDGK PQQEKGKATT FGSPTSFAIN KNSKKQKAAQ KFLDFASGKE GA.KLLAEVGV KIYFARKGMP SDESHKKPLT QIQLI

LSYSNYALRN

EAG lAY PDNL

METYYDRALR

TNVEWGIAEI

VPSYKTDEID

EF003-1 (SEQ ID NO:9) 5*55 *5SS *5 S

S.

a.

TAGGAGGACA

AIITAGCTG

GTTGCCGTC

AAAAAAGGGT

GTGCAACATG

AACAAAGAGA

TTCTATTCAA

CCTAGCGATC

AAATTAAAAG

AACATCACTr

ATCGCTATGG

GCGATCTTGT

AAAGGCGAAA

G'ITGCTGAAT

AAAGAATGAA

CCTGTGGGGG

AATTGGAATC

ACAAAATTAA

ACGAAGCGGA

AAAAAGCTGA

AAGAATACCA

CAACCAATGA

AAGGTGTCGG

TTGAAAGCAT

TGTTCTGCTA

TAGAAGATAA

AAGATAGCGA

ACATCAAGAA

GAAGTTTTAT

AAATAAACAA

TTCAAAAGAT

CATTATGGAA

TGCTAATTT

TTAGTGGCT

AGATGCGAA-A

AGGTCGTGCT

CTrTAACGGC

TGATTTACTG

CCCAGCCTAC

AGAAGCAAGT

AAAAATCAAG

TTAGCNACAT

GCAGACCAGA

ATCTTGGAGA

GTGAGCGACA

GCGCAACATC

GTGCAACCGA

GATTIACCTG

TTAGCAA'TT

ACGGTGGCAG

AATTAGCTA

TTAGAACCTG

AAACATTACG

GTTTTAAAAG

TCGCTGTIAT

AAGAAGACAA

TTGCCA-AGAA

ATcGTTGCCTA

AACCCTTCAT

TTTATTATT

AAAATGCCAA

TAAATGCAAA

ATGTCGTGGA

AAGCCTATGA

CTGGTTTAAC

CATTGCAAGT

AAGCGATGAC

TTCCTGCGTT

TGCAACAGT

GGAGATTACC

AGAAGCTGAG

CAACGATGCC

GGAAATGTTT

TGCTGGTGGT

AGTGrGGGATT

CGGCGTGATT

AAATCCTAAA

TGAAAAAGAC

AACGAAAGAT

TGTGACACGC

AACAAAAGAA

TTAA

AAATTCTAA.A GGCGCCAATA EF003-2 (SEQ ID NO:lO) 82 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

M"flKFYL ATFAVIATVI LAACGCNXQA DQKEDKEITV AVQLESSKDIl LEIAKXEAEK KGYKINIMEV SDNVAYNDAV QHDEADANFA QHQPFMEMFN KEKKADEJVAV QPIYYFAGGF YSKEYQDAKD LPENAKVGIP SOPTNEGRAL AILNANGVIK LKEGVGFNGT VADVVENPKN ITFESIDLLN L.AKAYDERDI AMVFCYPAYL EPAGLF1'KDA ILLEDKEASK HYALQVVTRK GEKDSEKIKV LKEAIM4I'EV AEYIKKNSKG ANIPAF EF003-3 (SEQ ID NO:11) CTGTGGGGG AAATAAACAA GCAGACCAGA AAGAAGACAA GGAGATTACC

GTTGCCGTGC

AAAAAAGGGT

GTGCAACATG

AACAAAGAGA

TTCTATTCAA

CCTAGCGATC

AAA ITAAAAG

AACATCACTT

ATCGCTATGG

GCGATCTTGT

AAAGGCGAAA

GTTGCTGAAT

AATTGCAATC

ACAAAAT'AA

ACGAAGCGGA

AAAAAGCTGA

AAGAATACCA

CAACCAATGA

AAGGTGTCGG

TTGAA.AGCAT

TGTTCTGCTA

TAGAAGATAA

AAGATACCGA

ACATCAAGAA

TI'CAAAAGAT

CATTATGGAA

TGCTAATTTT

TTTAGTGGCT

AGATGCGAAA

AGGTCGTGCT

CTI'TAACGGC

TGATTTACTC

CCCAGCCTAC

AGAAGCAAGT

AAAAATCAAG

AAATTCTAA.A

ATCTGGAGA

GTGAGCGACA

6CGCAACATC

GTGCAACCGA

GAT=ACCTG

TTAGCAATT

ACGGTGGCAG

AATTTAGCTA

TTAGAACCTG

AAACATTACG

GTTTAAAAG

GGCGCCAATA

TTGCCAACAA

ATrGTTGCCTA AACCC7TTCAT

TTATTATT

AAAAT13CCAA

TAA.ATGCAAA

ATGTCGTGGA

AAGCCTATGA

CTGGTTI'AAC

CATTGCA.AGT

AAGCGATGAC

TTCCTGCGTT

AGAAGCTGAG

CAACGATCC

GGAAATGTT

TGCTGGTGGT

AGTGGGC-ATT

CGCGCTGATT

AAATCCTAAA

TGAAAAAGAC

AACCAAAGAT

TGTGACACGC

AACAkAAAGAA

T

S

*5 5* S S S S

S

b 5S55

S

S. S. S

S.

EF003-4 (SEQ ID NO:12) CGGNKQA DQKEDKEITV AVQLESSKDI LEIAKKEAEXK KGYKINIMEV SDNVAYNDAV QHDEADANFA QHQPFMEMFN YSKEYQDAXD LPENAKVGIP SDPTNEGRAL *AILNANGVIX ITFESIDLLN LAKAYDEKOI AMVFCYPAYL EPAGLTTKDA GEKDSEKIKV LKEAMTTKEV AEYIKXNSKG ANIPAF KEKXADLVAV QP IYYFAGGF LKEGVGFNGT VADWVENPKN ILLEDKEASK HYALQVVTRK EF004-1 (SEQ ID NO:13)

TAAATCGAAA

ATTTGCAG

GAAACTGCTG

ACAGAAAACA

TTAGTAAAAA

GTCAFI'ACGG

GCGATAGAT

AATGTTGAAG

GAAGGATGAT

CTCTTCTCTC

CCCAGTCAAG

TAACCCAAC

TAGATCGITAA

TTAAGGTAAT

CCAACTCAGG

AAGTAGCCAA

AGAAATGAAA

TGCCTGTAC

CACTA'ITGAA

AGTTAAACAG

AAATAATGT'

TAATCATGAA

TACAGAGGCA

AACATTAGAA

CAGAACCATA

AAAATGATTA

AACGCAAAAA

GCTI'CAGACA

TTAGA.AGAAA

AAAGATGACA

GCAAAAAAAA

CAAA.AGACTG

AATGATAACG

CCAAAATCAA

AATTTGCAGG

ATAATACACA

GTAACGAAAA

AATTAACTC

CATCAGATAA

ATATGCAACA

CCATATACGG

ATGT'rAI=C

CTAAAAATGA

CATTGCTCTT

APLAGAAAG' CC

CGACCCTAAT

TGACGAGAAA

CCCTCACGCT

AATGCAGACT

AATTCAATTA

MTCATCACA

AAATAWI'ATT

CCTTACACGA ATGGGAACCA CCGTTAGTAA AATAA EF004-2 (SEQ ID NO:14) MKK MIKFAGIALI FAALLSACSN AKNNTQKKAE TAAQSSTIEA SDSNENEPNT ENITQAVKQL EE-KFNSDEXL VKIDVKNNVK DDTSDNP14AV ITVKVINDEA KKNMEEMQTA IDSNSGTEAQ KTAIYGIQLN VEEVAKTLEN DNDVISFITP YTNGNDRTIA KSTKNEN~IIP

LVK

EF004-3 (SEQ ID 83 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Gencs.

CTGTAGC AACCCAAAAA ATAATACACA AAAGAAAGCC GAAACTGCTG CCCAGTCAAG ACAGAAAACA TAACCCAAGC TTAGTAAAA-A TAGATGTTAA GTCATI'ACGG TTAAGGTAAT GCGATAGA'TT CCAACTCAGG AATCTTGAAG AAGTAGCCAA CCTTACACGA ATGGGAACGA CCGTITAGTAA AA

CACTATTGA.A

AcTAA-ACAC

AAATAATGTT

TAATGATGAA

TACAOAGGCA

AACATTAGAA

CAGAACCATA

GCTTCAGACA GTAACGA-AAA TTAGAAGAAA AATTTAACTC AAAGATGACA CATCAGATAA GCAAAAAAAA ATATGGAAGA CAAAAGACTG CCATATACGG AATGATAACG ATG-TTATTTC GCAAAATCAA cTAAkAAATGA

CGAGCCTAAT

TGACGAGAAA

CCCTCACGCT

AATGCAGACT

AATTCAAITA

TTTCATCACA

AAATATTATT

EF004-4 (SEQ ID NO:16) CSN AKNNTQKKAE TAAQSSTIEA SDSNENEPNT ENITQAVKQL EEKFNSDEI(L VKIDV.KNNVK DDTSDNPHAV ITVXVINDEA KKNME-.EMQTA IDSNSGTEAQ KTAIYGIQLN VEEVAKTLEN DNDVISFITP YTNGNDRTIA KSTKNENIIP

LVK

EF005-1 (SEQ ID NO:17) 00 9* 9 .09 6

TAAAAAATGA

GTTGGTTGt'G

CTAACTCTTT

GGATGGTCAG

GGACTGAAAG

ACTGCTCAAC

CCAGATTTAC

CAGGATATTG

GAATCCT ITG

TGGCCTGCAG

GTTGCCACAG

AGTATTTCAG

AAGAATGCTA

AATGATGTCA

AAAAACGATT

GTAAAAATCA

ACATI'GTGCG

ATGCGGTCCT

ATGTTGCCTT

TTATFI'AGA

GTGAATTTAA

CTGATGATCA

CCAATAGTGT

AAGACTATGC

AATCAGCCAA

CGTTG LTAGC

GTGTCACAAC

GCTACTTAGA

CACGATTGTG

GCAAGCAACG

CCACGGAAAA

A.ACACCAGAA

TCAAAATGCA

TCAAAATAAA

'TTrGGTAGC

AGGTGTTTCC

AGCTAAACTG

TACAATTACT

TTCTGCGAAT

AACTTTMT

AATTCATI'AC

AGCAGGCGAA

GGGATGCTTT

ACAAALAGAAA

ACCATGTTAA

GGTGAAA-AAG

TATAGTACTO

GCAGGCAAAG

TATGAAGGGG

TTAGAA~GAAT

GATCAACAGC

AAACGTTTGA

GGCAATG=T

GATGATTA

AAAA.ATGGCG

AAAGAATCAA

TTCTGGCCAT

AAGAGACAAA

ATACGACGGA

TTGTGACAGC

ATAGTGGCCG

ACCTTGAAGT

ATTTAAATAA

'TATGAAAAA

GCGAGGAAAG

AAAA.AGGCTr

TAGTGGTCTC

AAGTCCCAGA

AATATACTTT

AATA.A

TTTAGTAATG

ACCTGAAGAA

CCGCGTACAA

AACTGGGAT

CGCCTTGCAA

CGTGCGTGAC

GACALATGTGG

CATGACTCCT

CPLAGAATAAC

AGATAA.AATT

TCATGGCTTG

AGGCGGTTTG

GGATAPAGTC

see* EFOO5-2 (SEQ ID N'O:18) MKKRLTIVG MLFLAILVMV CGCKNQQATT KEKETKPEEL TLYIVRHGKT

MLNTTDRVQG

WSDAVLTPEG EKVVTATGIG LXDVAFQNAY sSDSGRALQT AQLILDQNKA GKDLEVVRDP DLREFNFGSY EGDLNKTMWQ DIADDQGVSL EEPMKENMTPE SFANSVAKLD QQREESKNW PAEDYATITK RLKKGLDKIV ATESANSGNG NVLVVSHGLS I SALLATLFD DFKVPEGGLK NASVTTIHYK NGE-YTLDKVN DVSYLE.AGEK ESK EFOO5-3 (SEQ ID NO:19) TTGTG GTAAAAATCA GCAAGCAACG ACAAAAGAAA AAGAGACAAA ACCTGAAGAA CTAACTCTIT ACATTGTGCG CCACCCAAAA ACCATGTAA ATACCACGGA CCGCGTACAA GGATGGTCAG ATGCGGTCCT AACACCAGAA GGTGAAAAAG TTGTGACAGC AACTGGGATT GGACTGAAAG ATGTTGCCTT TCAAAATGCA TATAGTAGTG ATAGTGGCCG CGCCTTGCAA ACTCCTCAAC TTATITTTAGA TCAAAATAAA GCAGG-CAAAG ACCTTCAAGT CGTGCGTGAC CCAGA TTAC GTGAATTTAA TTTTGGTAGC TATGAAGGGG ATTTAAATAA GACAATGTG CAGGATATTG CTGATGATCA AGGTGTTTCC TTAGA1AGAAT TTATGAAAAA CATGACTCCT TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

GAATCCTTTG

TGGCCT'GCAG

GTTGCCACAG

AGTATTTCAG

AAGAATGCTA

AATGAIrTCA

CCAATAGTGT

AAGACTATGC

AATCAGCCAA

CGTTGTTAGC

GTGTCACAAC

GCTACTTAGA

AGCTAAACTG

TACAATTACT

'ITCTGGGAAT

AACTTTATT

AATTCATTAC

AGCAGGCGAA

GATCAACAGC GCGAGGAAAC AAACGTTTGA AAAAAGGCTT GGCAATGTTT

TAGTGC-TCTC

GATGATTTTA

AAGTCCCAGA

AAAAATGGCG AATATACTTT AAAGAATCAA AA

CAACAATAAC

AGATAAAATT

TCATGGCTTG

AGGCGGTTTG

GGATAAAGTC

EF005-4 (SEQ ID CGKNQQATT KEKETKPEEL TLY IVRHGKT MLNTTDRVQG WSOAVLTPEG EKV'JTATGIG LKDVAFQNAY SSDSCRALQT AQLILDQNKA

GKDLEVVRDP

DLREFNFGSY EGDLNKTMWQ DIADDQGVSL EFMKNMTPE SFANSVAKLD

QQREESKNNW

PAEDYATITK RLKKGLDKIV ATESANSGNG NVLVVSHGLS ISALLATLFD

DFKVPEGGLK

NASVTTIHYK NGEYTLDVN DVSYLEAGEI< ESK0 EF006-1 (SEQ ID NO:21) ev 0 00.*0 S00 0000 0e

TAAACGATAA

GCAGTAGCTG

AAAG ETGGAG

GAAAAAGAAG

GCGTTGGAAA

GCGGTTAA-AG

GGGCTT1'ACT

GTTAGCTCTT

ATCACGCTGA

ACTAAAAAGT

AATGAAGAAG

CCGAAAAAAG

G ETCGTAAAG

AAAGAAGTCC

ATGGAGGGAA

TCTTAG'FITT

CTTCACCAGT

GCGTAAAATT

GTGGCGATAT

AAAATGATTA

CGAAAAAATA

CCGTTTCAGA

AAGAAGGGGT

TGAAATTCA.A

GGGCTGCGGT

ATGCGATTGC

AAGACGAAAA

AAGATTGGAT

TAAGATGAAA

AGGGGCTTGC

TCCACATGCA

AGAAGTGACG

CGATGCCAAC

TrGACTTTGTG

CAAATCGTTA

TTGGCCACGC

AGACCGGACA

TCATGAAAGT

TTTAATI'AAC

CTTAGAAAAA

CAACGAAAAT

TACGAAAAAA

AAACGTACAT

GGCAATAAAA

GAGATTTTAG

ACTTATACAG

TATTI'CCAAC

AATGCAGG;TG

CAAGAAATTC

GTATTkACTA

ACTGCTAC'T

GATCCAGCAA

TCAALACTTTG

GAAAGTTCAC

GTAAAAAAAT

TGGAACGGCG

TATGC-TCAGT

AGAGTGATGA

AACATGTAAA

ATTACGTGCT

ATGTGCCGTT

CTGATGGTTC

TCITAGAAGA

TCGATGATAT

TCATGACCAC

CCGTGGATCA

CTTATGCCAA

TAGTCAAAGT

CTATTGT'CC

AAT1'ACTGTA

CTLCGGTCTTG

ACCTTTATTA

ACCTAACAAG

cTTTAATGAA

AGAACCAGTT

AACGATTTAC

TGCTGGTTTA

TGATAAAAAT

TCTITATGAC

AGGATTAAAT

TATTATTGCG

GTTrACGTAGC

AGTCAATGAA

TAA

EF006-2 (SEQ ID NO:22) MKK RTI.WSVITVA VAVLVLGACG NKKSIDDSVLK VGASPVPH-AE ILEHV'KPLLE KEGVKLEVTT YTDYVLPNKA LESGDIDANY FQHVPFFNEA VKENDYDFVN

AGAIHLEPVG

LYSKKYKSLQ EIPDGSTIYV SSSVSDWPRV LTILEDAGLI TLKEGVDRTT

ATFDDIDKNT

KKLKvFNHESD PAIMTLYDN~ EEGAAVLINS NFAVDQGLNP KKDAIALEKE SSPYANI IAV RKEDENNMENV KKLVKVLRSK EVQDWITKKW NGAIVPVNE EF006-3 (SEQ ID NO:23) TTGC GGCAATAAAA AGAGTGATGA CTCGGTCTTG

AA.AGTTGGAG

GAAAAAGAAG

GCGTTGGAAA

GCGGT'AAAG

GGGCITTTACT

GTTAGCTCTT

ATCACGCTGA

ACTAAAAAGT

AATGAAGAAG

C LTCACCAGT

GCGTAAA.ATT

GTGGCGATAT

AAAATGAT'A

CGAAAAAATA

CCGTTTCAGA

AAGAAGCGT

TGAAATTCAA

GGGCTGCGGT

TCCACATGCA

AGAAGTGACG

CGATGCCA.AC

TGACTTTGTG

CAAATCGTTA

TTGGCCACGC

AGACCGGACA

TCATGAAAGT

TTTAATTAAC

GAGA'ITTTAG

AC~TTATAcAG

TATI'TCCAAC

AATGCXGGTb CAAGAAATrC

GTATTAACTA

ACTGCTACTT

GATCCAGCAA

TCAA.ACTTTG

AACATGTAAA

ATTACGTGCT

ATGTGCCGTT

CGATTCAT'T

CTcATGGTTC- TC?1'AGAAGA

TCGA'GATAT

TCATGACCAC

CCGTGGATCA

ACCTTTATTA

ACCTAACAAG

CTTTAATGAA

AGAAcCAGT

ALACGATTTAC

TGCTGGTTTA

TGATAAAAAT

TfC'ITTATrGAC

AGGATTAAAT

TABLE 1. Nucleotide and Amino Acid Seqeuences of E.JAecalis Genes.

CCGAAAAAAG ATGCGATTGC CTTAGAAAAA GAAAGT'--CAC CTTATGCCAA TATTATIGCG GTCGTAAAG AAGACGAAAA CAACGAAAAT GTAAAAAAAT TAGTCAAAGT GTTACGTAC AAAGAAGTCC AAGATTGGAT TACGAAAAAA TGGAACGGCG CTATTG'=C AGTCAATGAA EF006-4 (SEQ ID NO:24) CG NKKSODSVLK VGASP JPHAE ILEHVKPLLE KEGVKLEVTT YTDYVLPNKA LESGD IDANY FQHVPFFNEA LYSKKYKSLQ EIPDGSTIYV SSSVSDWPRV LTILEDAGLI KKLKFNHESD PAIMTTLYDN EEGAAVL INS NFAVDQGLNP RKEDENNENV KKLVKVLRSK EVQDWITKKW Di6AI-VPVNE:

VKENOYDFVN

TLKEGVDRTT

KKDAIALEKE

AGAI HLEPVG

ATFDDIDKNT

SSPYANI IAV EF008-1 (SEQ ID

TAAACCGTGA

ACACTTTTAG

AAAITAGCAA

GACAAAATTG

TTACCAGAAG

TTAGAAACAG

AATAAAGATT

CAAGAACAAA

GAAAACATL'C

AACGCGAAA.A

GCTGATATTC

TCCAAAGCTT

ACACCTGAAC

TTGTTGAAA

CCAA TI!ACG

TACTACAGCA

GAAAGAAATG

CAGGGTTAAC

TTGTGACAAC

AGCTGCATAG

ACATTGCGAA

GCGGAAATGG

AC=1TCTAC

CAGAAGATCC

GTGACGTGTT

ATTATACCCA

CTGATGATAA

ATGATIAAA

AAATGACCAC

CCAGTGTCGA

ATACACTTTT

TGATCAACTG

GAGGAATCAA

GTTAGCTGCT

GAACTCGATC

TATTGTGCCA

AGCTTCTGAA

CTGGTITAAC

AAGCAAAAAT

ACATGCTTGG

AGTAGAAAAA

AAAACTTAGC

AAAATTA ETA

TGCCGCTTAT

GATTATTGAT

TAAACGTAGT

CACAGACTCT

GAA ITTAACA CGAATGAAAA AATTTAGT= TGCGGGAATC AAGCCGCTGA CTATCTGATT TAGTGAAAAA ATTGGGACAG ACCCTCACGA

GCGGACATTI'

AAA'IT1AATGA

GTTACGCCAC

TTAGACATTG

GATCCAAAAA

AAACTACATG

GTTACAAGTG

ATTI'GGGAAA

ACCATTAAGA

ATGGAACGGG

TATTCTI'PA

AAACCGCCAA

AATATTTAAC

AAAATGGCAT

ATAAAGAT1T

AGGAAGCCAA

AAGGTGCCTT

'rTAACACAGA

AATCAAAAGC

TCTCAAAAGA

ATT'TTTTTA

AAAGAAAGAA

TGTTGGGCAA

ATATGAACCG

CGGCTTGAAC

AAAAGTTGAG

AAGTGCCGGT

TAAATATGTA

CTATACAGAA

AGCTAAAT

TAAATATTTC

AAGTCAAGGN

ACCTGTGTTA

AGTGAAACGA

CTTGCCAA.AG AACGAACAGA AGGCGATACG AAAATCCATG ATGGCTIAAT GAGTAAATAA EF008-2 (SEQ ID NO:26) t-(KFSLFFLT

KIELHSIVPI

KDYFSTSKNV

AKYTEKLSK

PEQMTTI IDT YSbZ2NWNLTK

LLAGLTLAAC

GTDPHEYEPL

TPQYLTSAGQ

LHEEAKAKFA

IKKSKAPVLF

IHDGLMSI(

GNQAAEKKEK

PEDIAKASEA

EQTEDPHAWL

DIPDDKKLLV

VETS VDKRSM LAIV'ITNS IL

DILFFNGLNL

DIENGIKY'VE

TSEGAFI(YFS

ERVSKEVKRP

SDLVKNVGQD

ETGGNG1WFNI<

NIRDVLVE-KD

KAYDLNAAYI

IYDTLFTDSL

1 M(TAKKVEN

PKN~KFYTEN

WEINTESQGT

AI(EGTEGD'IY

EF008-3 (SEQ ID NO:27) T TCGGGAATC AAGCCGCTGA AAAGAAAGAA

AAATTAGCAA

GACAAAATTG

TTACCAGAAG

TTAGA-AACAG

AATAAAGATT

CAAGAACAAA

GAAAACATTaC

AACGCGAAAA

GCTGATATC

TTGTGACAAC

AGCTGCATAG

ACATI'GCGAA

GCGGAAATGG

ACTTTTCTAC

CAGAAGATCC

GTGACGTG7T

ATTATACCGA

CTGATGATAA

GAACTCGATC

TATTGTGCCA

AGCTTCTGAA

CTGGTTTAAC

AAGCAAAAAT

ACATGCTTGG

AGTAGAAAAA

AAAACTTAGC

AAAA ITATTA CTATCTGATT TAGTGAAAAA rGGCA ATT'GGGACAG ACCCTCACGA ATATCAACCC GCGGACATTT TATTCTrrAA CGGCTTGAAC AAATTAATGA AAACGGCCAA AAAAGTTGAG GTTACGCCAC AATATTTAAC AAGTCCCGGT TTAGACATTG AAAATGGCAT TAAATATGTA GATCCAAAAkA ATAAACA'PIT CTATACAGAA AAACTACATG AGGAAGCCAA AGCTAAATTT GTTACAAGTG AAGGTGCCTT TAAATATI'TC 86 TABLE 1. Nucleotide and Am~ino Acid Seqeuences of E.faecalis Genes.

TCCAAAGCTT ATGATT1'AAA TGCCGCTTAT ATTTGGGAAA TTAACACAGA AAGTCAAGGN ACACCTGAAC AAATGACCAC GATTATTGAT ACCATTAAGA AATCAAAAGC ACCTGTGTTA

TTTGTTGAAA

CCAATTTACG

TACTACAGCA

CCAGTGTCGA

ATACACTTT

TGATGAACTC

TAAACGTAGT

CACAGACTCT

CAAT'TAACA

ATGGAACGGG

CTTGCCAAAG

AAAATCCATG

TCTCAAAAGA

AAGGAACAGA

ATGGCTTAAT

AGTGAAACCA

AGGCGATACG

GAGTAAA

EFOO8-4 (SEQ ID NO:28) C GNQAAEKKE( LAIVTTNSIL SOLVKNVGQD KIELHSTVPI GTDPHEYEPL PEDIAKASEA DILFFNGLNL KDYFSTSXNV TPQYLTSACQ EQTEDPHAWL DIENGIKYVE AKNYTEKLSK LHEEAKAKFA DIPDDKKLLV TSEGAFKYFS PEQM'FTI IDT IKKSKAPVLF VETSVDKRSM ERVSKEVKRP YSMM4NWNLTK IHIJGLMSK

ETGGNGWFNK

N IRDVLVERD KAYDLNAAY I

TYDTLFTD)SL

LMKTAKKVEN

PKNKDFYTEN

WEINTESQGT

AKEGTEGDTY

EF009-1 (SEQ ID NO:29)

TGACAA.ATGA

GCAGGTTGTG

GTAGGAACAA

GATLACAAT

GAAATTGT

GGAACGAAAT

AAATTGAAAG

ACGAATGGCG

GCGAAACAGC

ACTGAATTAG

AATAGCGGCA

CCTCTAAACG

GAGAATAAAC

ATTGCAGAAA

AAA.AATTTAG

CATCGGGGTC

AAAATGATrGA

TGGTAGAATT

TAAATGCCTT

TAGTATCAAT

ATATCACGAA

GGCGGGCGTT

AACTACCGAC

ATGCTACGCA

TGGCT'GTCGA

AAAAAGCGAA

T'ITATCAAAA

CATCAAAAGG

TAAAT1'AAT'T

TGTGAAGGAT

ATGGGAATCG

TACAGACTAT

TCAGCATCAA

TGGCAATACA

AATTrAAAGAC

AATTTTATTA

TGTCAGTGAT

AACAGCGCGC

TGCTGGGTAT

ACCTI'ATGTG

AGTTGTAGAA

CGCCAATGTT

GGACTTATTG

ACTAAGACAG

GTCAAAGACC

ACGCAACCAA

ATCTITI'TAG

GTCAATGCAC

GGCGGAGAAA

CAAACTGCAG

ATIACTGAAA

GCTTTACAAG

ACACCAGATA

AACATTGTCG

GAATATCAAC

CCAGCCTGGG

GGGTATTAGC

AAACCGTI'AA

GTI'TGAAAAA

ACGC.AGCATT

ACA.ATTACAA

CATITGGGAAT

TTGCTAITCC

GACTGATAAA

ATAAACGCCA

ATGTCGATGC

AAGATGCTAT

TGGCCCGAGA

AAGA-AGAAAC

AAACA G

TTTTACGATT

ACTAGGGGTT

GAAAAATATT

AGCAGAAAAA

TAAAGAGCAT

TTACGCTAAT

TAATGACCCA

AGTAGATCCT

ATTGAAAATA

LTCAGTGAT

TTCTTAGAA

AGAAGATCAA

GAAAAAGGTC

TAAAAAATAA

EF009-2 (SEQ ID MKKFSKLIG L IGVLAFTIA GCASGSVKDT KTETVKLGVV GTK(NDENESV KDRLKKKNID LQLVEFTDYT QPNAALAEXE IDLNAFQH-QI FLDNTYNKEHG TKLVSIGNTV NAPLGIYANK LKDITKIKDG GEIAIPNDPT NGGRAL ILLQ TAGLIKVDPA KQQLPTVSDI TENKRQLK IT ELDATQTARA LQDVDASVIN SGMAVDAGYT PDKDAIFLEP vNEK.AKPYvN ivvAREEDQE NKLYQKWVEE YQQEETKKVI AETSKGANVP AWETFGKK EF009-3 (SEQ ID NO:31) TTGTG CATCGGGGTC IGTGAAGGAT ACTAAGACAG AAACCGTTAA ACTAGGGGT

GTAGGAACAA

GAT'ITACAAT

GAAATGA'T

GCGAACGAAAT

AAATTGAAAG

ACGAATGGCG

GCGAAACAGC

ACTGAATTAG

AATAGCGGCA

CCTGTAAACG

AAAATGATGA

TGGTAGAATT

TAAATGCCTT

TAGTATCAAT

ATATCACGAA

GCGGGCGTr

AACTACCGAC

ATGCTACGCA

TGGCTGTCGA

AAA.AAGCGAA

ATGGGAATCG

TACAGACTAT

TCAGCATCAA

TGGCAATACA

AATTAAAGAC

AAITTTATTA

TGTCAGTGAT

AACAGCGCGC

TGCTGGGTAT

ACCTTATGTG

GTCAAAGACC GTTTGAAAAA GAAAAATATT

ACGCAACCAA

ATC TITTTAG

GTCAATGCAC

GGCGGAGAAA

CAAACTGCAG

ATTACTGAAA

GCTTTACA-AG

ACACCAGATA

AACAI1TGTCG

ACGCAGCATT

ACAATTACAA

CATTGGGAAT

TTGCTATTCC

GACTGATAAA

ATAAACGCCA

ATGTCGATGC

AAGATGCTAT

TGGCCCGAGA

AGCAGAAAAA

TAAAGAGCAT

TTAcGCTAAT

TAATGACCCA

AGTAGATCCT

ATI'GAAAATA

TTCAGTGATT

TTTCTTAGA-A

AGAAGATCAA

87 TABLE 1. Nucleotide and Amino Acid Seqeuences or E. faecalis Genes.

GAGAATAAAC TTTATCAAAA AGTTGTAGAA GAATATCAAC A.AGAAGAALAC GAAAALAGGTC ATTGCAGAAA CATCAAAAGG CGCCAATGTT CCAGCCTGGG AAACATTTGG TAAAAAA EF009-4 (SEQ ID NO:32) CASGSVKflT KTETVKLCVV GTKNDEWESV KORLKKKNID LQLVEFTDYT QPNAALAEXE IDLNAFQHQI FLONYNKEHG TKLVS7GNTV NAPLGIYANK LKDITKIXDG GEIAIPNDPT NGGRALILLQ TAGLIKVDPA KQQLP--1SDI TENKRQLKIT ELDATQTARA LQDVDASVIN SGMAVDAGYT PDKDAIFLE-P VNEKAKPYVN IVVAREEDQE NKLYQKVVEE YQQEETKKVI AETSKGANVP AWETFGKK EF010-1 (SEQ ID NO:33)

S.

S

S

T'GAA.AGAATA

CGTAGCTGGC

GCAGCGTCAA

CCAGATAATT

GCAAAATTAA

GTTGCTTCTG

AAAGGCGCCT

CAATTGCCAG

ATTTTAGGTA

TACAATTTAC

GAATTCCNTA

ACAGGTAAT

ACTGGCTCAC

GTCTTGCATC

CCATATGACT

TATGGTGATA

GAACCATTAA

AAAAACAAAG

AACGGACTTG

AAA7TTGA

TTAATCATIT

ATCGAAGAAG

ACTGAAATCA

ACTGTTGATC

GATAAAGTTC

AAATTGTACAC

GCACAGCTT

AAGGTGATGA

ATGACCAATT

AAATGGAATT

GTGAAAGCTA

ATGCTGATTT

ATAACTATAT

ACTCTTACGG

ATATTAGTAA

AAAANGANCC

ATGACTTCCC

cAAAAATTAT

AATGCTATAA

TAAATACCAA

,.CAATCTTAAC

AAACAACAGC

AAAAATCTGT

TTTATGGTGA

AAGATTACAC

GGCCAGAAGA

cAAAAGATI'C

CTAACGTI'QC

CAGAAGAAAC

AAA.AAGAAAT

;GAGGAAATA

kLGCTGTAGGA rAGTACACCA

A.ATCCATAAT

TGTTGGTT'GG

I'GATATTTCA

A.ACTGATTTA

TAAAGGAAAT

TCAACAAGTT

AGTCGATGGT

AAATATTGCT

TATTGGTAAC

TAACCAATAT

AGATGGCTTG

TACTTGGTTT

ACAAGCTGCT

TCAAGCGCAA

TGAATI'GTTA

AGAAGGCAAA

.ACCAACAACT

ATCTGTCACT

ACCAATTCTI

TACAGTTATG-

ACTTCCAAA-P

GCAAACACA-P

AGGAATGAPA

CTA-AGCGCCT

ACGTIATTAA

GCGAATAAAA

GGCGATTGGG

TTAGCACAAA

GCACCTAAAT

ACCA'IWAATC

TTAACTT ITA

AGTTATGAAA

GCTFrTCTA CAATATC CAT

GCCGACAAAG

ATCCAACAG

ATGCGTCAAG

GGCAAACCAC

ATCCCTAACT

GGT ETATTAA

CAATATGAAA

CATTTGAGTG

GAAGAAATG

GGTTTTACT'I

AACCGTTACCG

TTAATGGATC

TTAGACGAAM

AAATGGCAAA

CGTAAATC

TGTATCGTGT

TTATCGAGAA

ACCAAAAAAT

ATTATGCAAC

ATGCCAAAGA

GAAAACTGTA

ATAAAGAATA

GTGCAACGGA

TCGGCCAAAC

TTGCAGTAAA

ACATGXFTAA

ATGCTGCTAC

AAACACAAGG

TTGTTTCTCG

ATGTTGTTGC

ACAGCAATCC

AAGTTGGCGA

CTTGGAACAC

TTAAAGAACC

TTGTAAATGP

CAGCAAr.CTI]

ACCTAAAAAC

ATATCCAATC

AAGGATTAGC

TTCAAAAGAT

TGGGGACAAA

AAAAAT'rGGG

CTCAACAATC

GAATGCACA-A

AGCCTATGAT-

TGCCI'CCCA

TGTCGATAAA

AGI'CTAAAA

ATTCTTTGCA

AACAACTGAT

TPLACTTAAAA

AAGTACAACA

ACCTATGGAT

TCCACTAACA

AA.ACACGTCT

AGAATTGTTA

TGATCGTGTG

AGGAAACAAC

TGATAAGAC

LTAAAGTGAAA

AAATACAGGA

AGCTGGCTGG

TCAAAAATA

EF010-2 (SEQ ID NO:34) MAXRISR SWRTALAVGL SACGKSSKDA ASKGDDSTPT LLMYRVGDKP

ONYDQLIDNA

GAYADLTDLA

NLDISKVDGS

GSPKI INQYA

GDTILTQAAG

GLVYGEEGKQ

EEAKDSPILC

KVQKEMQTQL

NKI IEKKIGA

PKYAI(EAYDQ

YESATEVLKE

IDKDMINNLKV

KPLVSRPLTE

YEKVGDDRVK

FTFVNDKVKT

DEYIQSQK

KLKMEFVGWG

LPDNYIKGNT

FXKXXPNIAA

LHQWYKDGLI

PEJXTTAQAQM

LLIKDYTPTTH

EITNVATVMN

DWDQKMSTIV

INGKLYAFPI

FAIGQTFFAT

PTDAATSITP

ANYVVANTSK

LSAWNTGNNL

RYAASLJNTGT

ASGESYDI SL

LGNSYGQQVL

GNYDFPIGNQ

YDLNTNTWFM

NKIEKSVELLG

I IWPEESVTE

VDPEETLPKL

AQNYATNAQK

'FFNKEyVDKY ypFAVYCTTDT

RQETQGPMDY

LLNSNPELLN

EMrVKERD(S I

MDLKTAGWD

EFO1O-3 (SEQ ID TABLE 1. Nucleotide and Amino Acid Scqeuences of E. faecalis Genes.

CT CCTAAATC TTCAAA.AGAT

GCAGCGTCAA

CCACATAATT

GCAAAATTAA

GTTGCTTCTC

AAAGCCGCCT

CAATTGCCAC

ATTITTAGGTA

TACAATTTAG

GAATTCCNTA

ACAGGTAATT

ACTGGCTCAC

GTC TTCATC

CCATATGACT

TATGGTGATA

GAACCATTAA

AAAAACAAAG

AACGGACTTG

AAATTGTTGA

TTAATCA=T

ATCGAAGAAG

ACTGAAATCA

ACTGTTGATC

AAGGTGATGA

ATGACCAAT'T

AAATGGAATT

GTGAAAGCTA

ATGCTGA=T

ATAACTATAT

ACTCTTACGG

ATATTAGTAA

AAAANGANCC

ATGACTTCCC

CAAAAATI'AT

AATGGTATAA

TAAATACCAA

CAATCTTAAC

AAACAACACC

AAAAATCTGT

TTTATGGTGA

AAGATTACAC

CGCCAGAAGA

CAAAAGA'ITC

CTAACGTTGC

CAGAACAAAC

TAGTACACCA

AATCGATAAT

TTGTTG

TGATAI'TCA

AACTGATTA

TAAAGGAAAT

TCAACAAGTT

AGTCGATGGT

AAATATTGCT

TATTGGTAAC

TAACCAATAT

AGATGGCTTG

TACTTGGTT

ACAAGCTGCT

TCAAGCGCAA

TGA.ATTGTTA

AGAAGGCAAA

ACCAACAACT

ATCTCGTCACT

ACCAATTCTT

TACAGTTATG

ACTTCCAAAA

GCAAACACAA

ACGTTATTAA

GCGAATAAAA

GGCGATTGr.G

TTAGCACAAA

GCACCTAAAT

ACCATTAATG

TTAACTA

AGTTATGAAA

GCTTTTGCTA

CAATATCCAT

GCCGACIAAAG

ATTCCAACAG

ATGCGTCAAG

GGCAAACCAC

ATCGCTAACT

GGTTTATTAA

CAATATGAAA

CATTTGAGTG

GAAGAAATGG

GGT'ITITACTT'

AACCGTTACG

TTAATCGATG

TTAGACGAAT

TGTATCGTGT

TrATCGAGAA

ACCAAAAAAT

ATTrATGCAAC

ATGCCAAAGA

GAAAACTGTA

ATAA.AGAATA

GTGCAACGGA

TCGCCCAAAC

TTGCAGTAAA

ACATGATTAA

ATGCTt''CTAC

AAACACAAGG

TTGTTI'CTCG

A'TGTTGTTGC

ACAGCAATCC

AAGTTGGCGA

CTTGGAACAC

TrAAAGAACG 1-rGTAAATGA

CAGCAAGCTT

AC CTAAAAAC

ATATCCA-ATC

TGGGGACAALA

AAAAATTCG

GTCAACAATC

GAATGCACAA

AGCCTATGAT

TGCGTTCCCA

TGTCGATAAA

AGTTCTAAAA

ATTCTTTCCA

AACAACTGAT

TAACTTAAAA

AAGTACAACA

ACCTATGGAT

TCCACTAACA

AAACACGTCT

AGAATTGTI'A

TGATCGTGTG

AGGAAACAAC

TCATAAGAC

TAAAGTGAAA

AAATACAGGA

AGCTGGCTG

TCAAAAA

p GATAAAGTTC AAAAAGAAAT EF010-4 (SEQ ID NO:36) CGKSSKDA ASKGDDSTPT LLMYRVGDKP DNYDQL IDNA

GAYADLTDLA

NLDI SKVDCS

GSPKIINQYA

GDTILTQAAG

GLVYGEEGKQ

EEAKOSPILG

KVQKEMQTQL

NKI IEKKIGA

PKYAKEAYDQ

YESATEVLKE

DKOMINNLKV

KPLVSRPLTE

YEKVGDDRVK

FTFVNDKVKT

DEYIQSQK

KLKMEFVGWG

LPDNYIKGNT

FXKXXPNIAA

LHQWYKDGL.I

PLKTTAQAQM

LLKDYTPTTH

EITNVATVMN

DWDQKMSTIV

INGKLYAFP I

FAIGQTFFAT

PTDAATSTTP

ANYVVANTSI(

LSAWNTGNNL

RYAASLNTGT

ASGESYDISL

LGNSYGQQVL

GNYDFP IGNQ

YDLNTNTWFM

NKEKSVELLG

I IWPEESVTE VDPEEThPKL

AQNYATNAQK

TFNKEYVDKY

YPFAVKTTDT

RQETQCPMDY

LLNSNPELLN

EMVKERDKS I

MDDLKTAGWD

EF011-1 (SEQ ID NO:37)

TAACGIIT

ATGGGACTGT

ACAGAAACAA

CCCAAAAATC

CTAGGT-GTCG

AAATACCAAA

CAACTAAAAC

AAAGCCATTG

AAACAAAATA

ATAACTCGCT

AATGCGCTTG

TTCGGTTTAA

GAGAAAAG

TAATGTTAAG

CAGCTAAAAC

CTAAGAAACT

GTGACCGCGT

AAGTTGAATC

CAGACTTAAT

CGCCAACCAT

TCGAAACGTT

TAGAAAAAGA

TTTGTTAGT

TTCATGATAC

AATGAAAAAG

TGCTTGTCAA

GGAAGTCACA

CCTTCTTTT

GGTAGGTGCG

AGCAGGCGGC

TATrATTTCT

TTACTTAGCT

AGGCACTATT

AATI'GCTGAC

TAACCAAGGA

ATTTGGCTTC

AAATT LTTAG

ACAAATAAA

GTCAAAGACA

GATAATCG T

CCAACTAAAA

ATTAAAGAAC

GGTCGTCAAC

GTACATGCCA

TTTGATAAAG

GTGAAAAAAC

CAAC'rTTCCG

AAAGCAGCAG

CAATGATGGC

AAACAGCAGA

CCAkATGGTCA

CCTTGGATAC

ATATCCCTGC

CAGATIrAGA AAGATrATCA AAAA'rCC7'rC-

AAGAGGTAGC

AAGCAGAAGC

CTTACGGA.AA

ACGATAAGAT

AGTrTCAATG

TTCTGCA-ACA

ATTAACCGT

AATGGATGCA

GTAFFGAAA

AAAAATCAAT

AGAACAATTA

GCCATCAACG

TAAAGAAAAA.

TAGCGCGAAT

ACGCTCTCGT

TGAAGCTTCC

89 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.f[aecalis Gencs.

ACTCATGGC

GTGGTAGATC

GAATTGATTC

GTTTGGTATC

AAAGGATTAG

AAAGTG'FITC TTACGAATAT GTFI'TAGAAA AAAATCCTGG GATTCTCTPI' GCACCAAAGC AATTGGTCGC GACGATTCAA AAGATAACGT CGCTGCPAAAC AAAAAACCGA TGCTGGTAA-A AATGATAAAG TCAITATGCT TCAACCAGAT TAAGCGGTGG TGGATTAGAA TCAATGCATT TGATGATAGA AGATGTTAAA

AGTAA

EF011.-2 (SEQ ID NO:38) bMflKK FLAMMAVSM GLLMLSACQT NKKTADSATT ETTAI(TEVTV KDTNGQLTVP KNPXKVVVFD NGSLDTMDAL GVGDRVVGAP TKNIPAYLKK YQKVE-SAGGI KEPOLEKINQ LKPDL II ISG RQQDYQEQLK AIAPT IYLAV DAKNPWASTK QNI ETLGTIF DKEEVAKEKI TGLEKEIADV KKQAEASANN ALVVLVNEGQ L SAYGKGSRF GLIHE)TFG;F AADDKIEAST HGQSVSYEYV LEKNPGILFV VDRTKAIGGD DSKDNVAANE LIQKTDAGKN DKVIMLQPDV WYLSGGGLES MHLMIEDVKK GLE EF011-3 (SEQ ID NO:39) TTGTCAA ACAAATAAAA AAACAGCAGA TTCTGCAACA

ACAGAAACAA

CCCAAAAATC

CTAGGTGTCG

AAATACCAAA

CAACTAAAAC

AAAGCCATTG

AAACAAAATA

ATAACTGGCT

AATGCGCTTG

ITCGGTTTAA

ACTCATGGGC

GTGCTAGATC

GAATTGATTC

GTTTGGTATC

AAAGGATTAG

CAGCTAAAAC

CTAAGAAAGT

GTGACCGCGT

AAG'ITGAATC

CAGACTTAAT

CGCCAACCAT

TCGAAACCTT

TAGAAAAAGA

TTGTGTTAGT

TTATGATAC

AAAGTGITTC

GCACCAAAGC

AAAAAACCGA

TAAGCGGTGG

AG

GGAAGTCACA

CGTTG'TI'T

GGTAGGTGCG

AGCAGGCGGC

TATTAT'TCT

TTACTTAGCT

AGGCACTATT

AAITGCTGAC

TAACGAAGGA

ATTCGCTTC

TTACGAATAT

AATTGGTGGC

TGCTGGTAAA

TGGATTAGA-A

GTCA-AAGACA

GATAATGGTT

CCAACTAAAA

ATTAAAGAAC

GGTCGTCAAC

GTAGATGCCA

TTGATAAAG

GTGAAAAAAC

CAACTTTCCG

AAAGCAGCAG

GTTTTAGAAA

GACGATTCAA

AATGATAAAG

TCAATGCATT

CCAATCGTCA ATTAACCGTT CCTTGGATAC AATGGATGCA ATATCCCTGC GTATTTGAAA CAGATTTAGA AAAAATCAAT ALAGATTATCA AGAACAATTA AAAATCC'ITG GGCATCAACG AAGAGCTAGC TAAAGAAAAA AAGCAGAAGC TAGCGCGAAT CTTACGGAAA AGGCTCTCGT AC-GATAAGAT TGAAGCTTCC AAAATCCTGG GATTCTCTTT AAGATAACGT CGCTGCAAAC TCATTATGCT TCAACCAGAT TGATGATAGA AGATGTTAAA EF011-4 (SEQ ID CQT NKKTADSAT1' ETITAKTEVTV KDTNGQLTVP KN4PKKVVVFD NGSLDTMDAL GVGDRVVGAP TKNIPAYLKK LKPDLIIISG RQQDYQEQLK AIAPTIYLAV DAKNPWASTK TGLEKEIADV KKQAEASANN ALVVLVNEGQ LSAYGKGSRF HGQSVSYEYV LEKNPGILFV VDRTKAIGGD DSKDN'JAANE WYLSGGGLES MHLMIEDVKK GLE YQKVESAGG I

QNIE:TLGTIF

GLIHDTFGFK

LIQKTDAGKN

KEPDLEKINQ

DKEEVAKEK I

AADDKIEAST

DKVIMLQDV

EF012-1 (SEQ ID NO:41)

TGAGGGGGCA

CTITTAGCCG

GCAGCTGAAC

CAAACAACAG

TTTGATGATG

ACAACATGAA

CATGTIGGCGG

AAAAAATCAG

ATAAAAATAC

ATAGTGCCAC

ATTGGGGAAA

AACCAAAGAA

TATTAGTTCA

CTIACAATG

GGTGCCAGCT

AAAGTAGTAG

GCGGCAGAGA

CCTGCACCAA

GCACAACAFI'

CTAGCTAAAG

GTTTIGATTGC

AAGTAGAITC

TCTCAACAT

TATTTGAAGG

ATGTCAAGAT

AACAGGGT

GGGAAATA.

GGATACAACA

CCTTTATCGG

TAGTGACGAT

TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

GGGCGCAAGT

ACGGCCCAAG

AATGCCTATT

GTCGATGAAT

GCCCAACCTT

T= GTCGAAG

CCATTTACGC

GAATACTATG

GATAATACTO

CAATATGTC

TACTTCTTAG

GCGATTGGCC

AAACCCCTTA

TCCGAGCTT

ACGAAAGCCC

GATCAAGGAA

TTAGAAATTA

AATTATGAGT

AACTTATATG

CAATT-GTAG

TACAAAGAAG

AGTGCCTCAA

GATTAT'ITCC

ACCACTITAC

ATTTTGTTTA

TACTAGACAG

TAGGGATTTC

CCTTC'ITAGC

CGCAAGGCAA

TAGCCAATTG

ATGCGGATCA

GGATTAAC'T

AACAATATCA

ATI'TCAACAA

AAGCAATTGA

ACGGATTGAT

ACAGTGGCGA

AAGCGGATGT

AACGAATITGC

CCAT'ITCATC

TGTCTCTTTC

CAGGAGAATC

AAGAGGCACG

CGGAAGACAT

ATTATCTAAT

AC'ITGCGCAA

CTT'GGGAG

TTCTTGGAAA

TGTTAAAAAT

AGCCCCGAAT

AGTCGTTTCG

AGATTACGCC

GGATGCGACT

AGTGAAACTG

ATATCAAGTG

AGATGATCCA

AAAAGAAGGA

TAAAGAAGCC

TCCAAGTAAA

ATAI'rTGAAA

CGGTAAAAAA

TGAATATGTT

GCAACCAAGT

AGGATGGATT

AAGTTACAAT

AACGATTAAT

CTTGTTGAAC

CAATCCTAAA

TGCCTATI'TA

GGGATTAAGT

AAACTGGTGA

AGTTTTGAAA

GACAAAGAAT

ATTGCTT

TTGATAGTG

TCAGATACTT

GAAGAAGTTG

AATGAACTAG

GGCTATGTCA

ACGCCATrAG TTAiACACAAA

CTT'TATGCGA

AATGACGTCA

GTGAAA?=

CAAAGTCAGT

AATAATGTGA

GCCGGCAGTA

TACGGCAATT

GCCAATAATC

CAAGATGCTG

TTGAAAGGCA

ACAGAATCA

GGAGCAACG

CACCAGCCAC

TACGCAACGG

TCATTGTTGA

TAGCGOCACA

AACATTTACT

GGACATTGA

CGGTTAGCAC

ACTTAGTTCG

GTCATCCAGA

CGAATGTITCA

GTGTCTTAAA

ATCCAGAAAC

AAAAAG;tTCA

CATTGCTGGC

TGCAAGAAAA

ACCAATCGCG

GTGAATTAGA

ATCATAATIC

CAGAGAAACA

C CCAA'-TACC

TTAGTTATCA

'CGAGCCAATC

GATTGGACCG

TGAAAAGTCA

A'ITIAAAACAG

AAATCAAAAA

TTATAGCGGG

AAAAAATCCA

AATCAAAGAA

CATTAACGGA

TGTGGCCAkAC

TTTACGAAAA

CGATGGGTCA

GGATGAAGAT

AGCTGAATGG

GGCAGACACA

TCTGCCAGGT

ACGTGAAAAA

CTCTTACTTT

CAAATACGAC

GTTr~TGCAGAA

GCTCTATCAA

CTTGTATGGG

9* EF012-2 (SEQ ID NO:42) MKLGKK VVGL.IATGFL LAACGGTKEA AEKrVDSGNLA AEQK IS ISSP AP: -STLDTTQ

TTDKNTFTKA

AQDFVYSWKK

QPSFLAVVSI

YYDADQVKLE

FLDFNKKEGT

RAYSGEYLKN

EITISSQPSN

LVEEARTINA

YFHLRNAYLT

QHLFEGLYRF

LVTPATIGPN

AWLAPQNQKF

EVAVSTIKED

PLANV!4LRKA

DVKKAQAEWT

NVNQSRREKN

NNPEKQFAEY

DDDSATVPAL

AYLLDSVKNS

VEAQGKDYAL

NTG INLYQVN

IGQAIDKEAL

KAQADVGKKV

YELSLSGWIA

KEAEDILLNQ

AI(DVKI SDDG.

FEI RNGEXSV

DSEHLLYSGP

ELDLVRINGQ

TQSVLJNDCSK

KLSLLAAD)TD

GSSELDSYFN

DAAQVPLYQS

RKYHFTLR.EG

DELGISAPND

FTLANWDATS

YVQQYQDDPG

PLNGLIPSKL

QGKRIAZYVQ

LYAGESSYNY

ASNYLIINPKL

IKWSNGEPIT

KEFIVELKQA

DTWTLKKNPE

YVSHPDVANY

YANPETDEDF

SQL.QENLPGL

GNYHNAKYIDQ

KG ISYHLYGD EF012-3 (SEQ ID NO:43) ATGTGGCGG AACCAAAGAA GCGGCAGAGA AAGTAGATTC GGGAAATA

GCAGCTCAAC

CAAACAACAG

=rGATGATG

GGGCGCAAGT

ACGGCCCAAG

AATGCCTATT

GTCGATGAAT

GCCCAACCT'

rrl'TCGAAG

CCATTTACCC

GAATACTATG

GATAATACTG

CAATATGTTC

AAAAAATCAG

ATAAAAATAC

ATAGTGCCAC

ACCACTTTAC

ATTTTGTTTA

TACTAGACAG

TAGGGATTTC

CCTTICTTAGC

CGCAAGGCAA

TAGCCAAT'G

ATGCGGATCA

GGATTAACTT

AACAATATCA

TATTACTTCA

CTI'TACA-ATG

GGTGCCAGCT

CTTGCGGGAG

TTCTTGGAAA

TGTTAAAAAkT

ACCCCGAAT

AGTCGTTTCG

AGATTACCCC

GGATGCGACT

AGTGAAACTG

ATATCAAGTG

AGATGATC CA

CCTGCACCAA

GCACAACATT

CTAGCTAAAG

GGGATTAAGT

AAACTGGTGA

AGTTTTGAAA

GACAAAGAAT

ATTGCTqIGGT rrGGATAGTG

TCAGATACTT

GAAGAAG7MI~

AATGA.ACTAG

GGCTATGTCA

TCTCAACATT

TATI=AAGG

ATGTCAAGAT

GGAGCAACGG

CACCACCGAC

TACGCAACGG

TCA TGTTGA

TAGCGCCACA

AACATTTACT

GGACA'GAA

CGGTTAGCAC

ACTrAGTTCG

GTCATCCAGA

GGATACAACA

CCT1'TATCGG

TAGTGACGAT

CGAGCCAATC

GATTGdACCG

TGAAAAGTCA

ATTAAAACAG

AALATCAAAAA

TTATAGCGGG

AAAAAATCCA

AATCAAAGAA

CATTAACGGA

TGTGGCCAAC

91 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

TACTTCTTAG

GCGATTGGCC

AAACCCCTTA

TTCCGAOCTT

ACGAAAGCCC

GATCAAGGAA

TTAGAAATTA

AATTATGAGT

AACTTATATG

CAATTGGTAG

TACAAAGAAG

AGTGCCTCAA

ATTCAACAA

AAGCAATTGA

ACGGATTGAT

ACAGTGCGA

AAGCGGATGT

AACGAATTGC

CCATITCATC

TGTCTCTTC

CAGGAGAATC

AACAGGCACG

CGGAAGACAT

AkTTATCTAAT

AAAAGAAGGA

TAAAGAACCC

TCCA.AGTAAA

ATATTGAAA

CGGTAAAAAA

TGAATATGTT

GCAACCAAGT

AGGATGGATT

A.AGTTACAAT

ALACGATTAAT

CTTGTTGAAC

CAATCCTAAA

TGCCTATTTA

ACGCCATTAG

TTAACACAAA

CTTTrATGCC-A

AATGACGTCA

GTGAAACT'fT

CAAAGTCAGT

AATAATGIGA

GCCGGCAGTA

TACGGCAATI

GCCAATAATC

CAAOATGCTG

TT GAAAGGCA

ACAGAA

CGAATrG'ITCA

GTGTCTTAAA

ATCCAGAAAC

AAAA.AGCTCA

CA TGCTGGC

TGCA.AGAAAA

ACCAATCGCG

GTGAATTAGA

ATCATATGC

CAGAGAAACA

CC CAAGTACC

TTAGTTATCA

=PIACGAAAA

CGATCGGTCA

GGATGAAGAT

AGCTGAATGG

OCCAGACACA

TCTCCCAGGT

ACGTGAAAAA

CTCTTACTTI'

CAA.ATACGAC

GTTGCAGAA

GCTGTATCAA

CTTGTATGGG

GATTATTTCC ACTTGCGCAA EF012-4 (SEQ ID NO:44) CGGTKEA AEKVDSGNLA AEQKISISSP APISTLDTTQ

ITDKNTFTMA

AQDFVYSWKK

QPSFLAVVSI

YYDADQVKLE

FLDFN'KYKEGT

RAYSGEYLKN

EITISSQPSN

L'JEEARTINA

QHLFEGLYRF

LVTPATIGPN

AWLAPQNQKF

EVAVSTIKED

PLANVHLRXA

OVKKAQAEWT

NVNQSRREKN

NNPEKQFAEY

DDDSATVPAL

AYLLDSVKNS

VEAQGKDYAL

N~TG INLYQVN

IGQAIDKEAL

KAQADVGKKV

YELSLSGWIA

KE-AEDILLNQ

AKDVKISDDG

FEIRNGEKSV

DSEHLLYSrCP

ELDLVRINGQ

TQSVLNDGSK

KLSLLA-ADTD

GSSELOSYFN

DAAQVPLYQS

RKYH-FTLREG

DELGISAPND

FTLANDATS

Y-VQQYQDDPG

PLNGLIPSKL

QGKR IAEYVQ

LYAGESSYNY

ASNYLINPKL

IKWSNGEP IT KEF IVELKQA

DTWTL.KKNPE

YVSHPDVANY

YANPETDEDF

SQLQENLPGL

GNYHNAKYDQ

KGISYHLYGD

YFHLRNAYLT E EF013-1 (SEQ ID

TAACGAAAAA

AGTCTAGCAG

GAAACAAAGA

TCTGTCACGG

CCTTCTGCAG

ATTACAATTA

CCTAATGCAA

TATGCGGATG

ITAATCGCAA

GGACAAGAAG

TCTGGTAAAA

ATTTTAGCAA

GGCACAAAAA

GA I=ACCAA

TGAAAAAAAT

GATGTGGAAA

AAGCCACGCA

GTCAAAATTC

CAACTGATGA

CCAATGTTGT

CCATTACAGC

CCAATGGCAA

CCGTTGATCA

CAGCGCTTTC

CAGCACCGAA

GTTTTACTGC

ATCGCTTAGA

ATTAA

TGCTTTGTTC

CAAAAAAACA

GAAATCCTCT

TAATGTGACA

AACGAATGAA

TTTCAATCCT

AACGGTAGTC

TTTTACAGTA

ACGGAATAGT

TTTTAGCAAT

TGCAACTATT

GGATGCCCAA

TGTTACGI A

AGTATGTTAA

GCAAGCACAA

AGCCAACAAG

GGGGAAAATC

GTCCCTCAAA

GAAAGAAATG

GGTGATGCTT

ATTAGTCCCA

GCACCTGTCC

ATTACGATTG

TTAGTGTCAC

GGGAATTTCA

AATGGAGAAA

CGTTCAGTGT

ATGAT'rCTAA

AAATGAAAAG

CGTCAGAAAA

ACCAAGCACC

AA.ATTAATGG

CTGCACAAGC

GAGCGGGAGC

AAA'FIGATAT

ATCCGAAACA

GTGCAGATGA

CAGCCAGTAA

TAGGGACACC

ATTGTCTTTA

GCCAAAGCAA

TAGTCATTCG

TGCCACGCAG

TGATACAAAC

TACTACATTA

AGGTG ITTTT

GACTACTCAA

TCCAAGTTCA

ACGGACAATT

TGCGCGGGTG

LIAGTITCC C

TTACTTGTFI'

EF013-2 (SEQ ID NO:46) NKKIALFS MLTFSVLSLS LAGCGNKKTA STNDSKPKQE TKKATQKSSS QQEMKSSHSS VTGQNSNVTG ENPSENATQP SAGTDETNEV PQNQAPDTNI TITNWVFNPE RNEINGTTLP NATITATVVG DASAQAGVFY ADANGNFTVI SPPRAGATTQL IAT JDQRNSA PVQIDIPSSG QEAALSFSNI TIDPKQGTIS CKTAPNATIL VSRADDARVI LASFTADAQG NFTASNLVPG TKNRLDVTLN GEIGTPYLFO LPN 92 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

EF013-3 (SEQ ID NO:47) ATGTGGAAA CAAAAAAACA GCAAGCACAA ATGATTCTAA GCCAAAGCA.A

GAAACAAAGA

TCTGTCACGG

CCTTCTGCAG

ATT ACAATTA

CCTAATGCAA

TATGCGGATG

TTAATCGCAA

GGACAAGAAG

TCTGGTAAAA

ATTTTAGCAA

GGCACAAAAA

AAGCCACGCA

GTCAAAATTC

GAACTGATGA

CCAATGTT1GT

CCA'ITACAC

CCAATGGCA.A

CCGTTGATCA

CAGCGCTITC

CAGCACCGAA

GTTTACTGC

ATCGCTTAGA

GAAATC-CTCT

TAATGTGACA

AACGAATGAA

TTTCAATCCT

AACGGTAGTC

TTTTACAGTA

ACGGAATAGT

TTTTAGCAAT

TGCAACTATT

GGATGCCCAA

TGTI'ACGI'A

AGCCAACAAG

GGGGAAAATC

GTCCCTCAAA

GAAAGAAATG

GGTGATGCTr

ATTAGTCCCA

GCACCTGTCC

A-TTACG;ATG

TTAGTGTCAC

GGGAATTTCA

AATGGAGAAA

AAATGAAAAG

CCTCACAAAA

ACCAAGCACC

AAATTAATGG

CTGC-ACAAGC

GAGCGGGAGC

AAATTGATAT

ATCCGAAACA

GTGCAGATGA

CAGC CAGTAA TAGG-dACACC

TAGTCATTICG

TGCCACGCAG

TGATACAAAC

TACTACATTA

AGGTGT'TTTT

GACTACTCAA

TCCAAGTTCA

AGGGACAATT

TGCGCGGGTG

TTTAGTTCCC

TTACTTGTTT

S.,

S

GATTTACCAA AT EF013-4 (SEQ ID NO:48) CGNKKTA STNDSKPKQE TKKATQKSSS QQEMKSSHSS VTGQNSNVTG ENrPSENATQP SAGTDETNEV PQNQAPDTNI TITNVVFNPE RNEINGTTLP NATITATVVG DASAQAGVFY ADANGNFTVI SPRAGATTQL IATVDQRNSA PVQIDIPSSG QEAALSFSNI TIDPKQGTIS GKTAPNATIL VSRADDARVI LASFTADAQG NFTASNLVPG TKNRLDVTLN GEIGTPYLFO LPN EF014-1 (SEQ ID NO:49)

TGATGGTCCA

AGCTTAA'rrA

GTAGCGGGTA

TATAATGAAG

ATTGAACGGA

AAGACGAAGA

AAAAATTACC

TCTGTTAACA

TTTTTATCT

GCGTTTGATC

CATTTAGATG

ATGCGTGGAT

CAATCAATCA

GTGGATTCCA

AAACAACAGC

CTATACCCAG

AAGCCAGATG

CAAAGTGATT

GGCAATACGT

ACGGAAAATG

rGACTTTTTAA

GTTTAGTCAT

GCTATTTAAA

CGCAAAATAA

AATTAGGCAC

AAATAACCTA

AAGGGATGCA

CAGTTGAGAA

TTATTAAGTT

GTGTCACCA.A

GTACGAA.AGC

TCCGACAACA

TGAAAATAAT

ATGAATTGAC

TTTCTTTTGA

ATAGTATTGA

AACGAGATCA

ATACCGTTCA

ATATTGGTGT

GCTTTATAAA

GAGAGAGGAA

CATTTTGGTT

GAAAACAATT

AGATACTCAA

AACTAGGACT

TTTAAGTTTG

GCGAATTGAA

ATTATTGAAT

AATTGATGCG

AGACGGGCCA

'TrTATCTTAT

AGAAATTATT

GGACATTATT

TCATTTAGTC

CTGGCGCACT

AAATGTCCGT

AGACGGCTAT

AGATGAAGCA

TCCTGGTAAT

ATAA

GTACAGCCAA

TTTGTCACAG

GATAAAGGCT

TCCTTTTTGA

GATGCTATGA

CCACGGGATA

CCCCCTATA

ATTCCAATCA

GTTGGCGGCA

GGATCCATTC

GCCCGTGAAA

CAAGCAGTTG

GATTCGTTAA

AAAGAAGGTT

TTAGTAATG

CATCAATTAC

GTCTTCCATA

GCTGAGGAAA

ACACAGACCG

TCAGTAGGAA

TCGGCTCAGC

ATGTTCCCAT

'ITATGGGGCT

TGGTGATTAC

GTTTTGTTCA

CCTACGATGG

ATCATI'ACGT

TAGA2'GTCAA ArTC=ATGC

GACATAGCGA

AAGACAAGTT

ATGGAAACAT

TGACTTGGAC

AAGGGCGCAG

GTGTGTCTTT

CGAACGGTGA

ACGAAAT1GAC

GCCCGTTGCC

GCGAA.AAATC

ATACTTTGCT

AAAAAATGAT

AGACAATACA

CGTGAATAAC

A.ATTGATGCG

ACCAACACCT

TGTGTT1'AAC

TGTCAAGCAG

AGGGAAACAG

TAACGATATT

GAAATCTGGT

TCAAACTGAT

cAATTATGAT

TATGGTTGAA

AAATTTAGAA

ATTTITTATAT

TTCCATCAAC

ATCAGTTAA

S

a.

EF014-2 (SEQ ID NO:SO) MSRKRRIS LISLVIILVF VTVGSAYFAV AGSYLKKTID KGYVPIKNDY NEAQNKDSQS FLIMGLDNTI ERKLGTTRTD AMMVITVNNK TKKITYLSLP NYQGMQRIEA AYTYDGPTAS VNTVEKLLNI PINHyvvFNF LSFIKLIDAV FDGVTKDGPG SIHFDACKQH LDGTKALSYA RERHSDNDIM RGFRQQEIIQ SIMXIMDIID SLNGNIQTDV DSNELTHLVK EGLTWTNYDK QQLSFDWRTF RDSFVQ IDAK

GGIDVNVKQA

AVE-DKLKSGQ

SNEGRsMVEL 93 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

YPOSIENVR{ QLRVSLNLEK PDERDQDCYV FHTNGEFLYQ S:YTVQDZEAA EENEb4TSING NTYIGVPGNT QTGPLPSVKT

ENGFIK

EF014-3 (SEQ 10 NO:51)

TGCT

GTAGCGGGTA

TATAATCAAG

ATTGAACGGA

AAGACGAAGA

AAAAATTACC

TCTGTTAACA

TTTTTATCTT

GCGTTTGATC

CATTTAGATC

ATGCGTGGAT

CAATCAATCA

GTGGATTCCA

AAACAACAGC

CTATACCCAG

AAGCCAGATG

CAAAGTGATT

GGCAATACGT

ACGGAALAATG

GcTATTTAAA

CGCAAAATAA

AA'ITAGGCAC

AAATAACCTA

AAGGGATGCA

CAGTTGAGAA

TTATTAAGTT

GTGTCACCAA

GTACGAA-A-C

TCCGACAACA

TGAAA.ATAAT

ATGAATTGAC

TTTCTTTTGA

ATAGTATTGA

AACGAGATCA

ATACCGTTCA

ATATTU'GTGT

GAAAACAATT

AGATAGTCAA

AACTAGGACT

TTTAAGT'G

GCGAATTGAA

ATTATTGAAT

AATTGATGCG

AGACGGGCCA

TTTATCTTAT

AGAALATTATT

GGACATTATT

TCAFI'TAGTC

CTGGCGCACT

AAATGTCCGT

AGACGGCTAT

AGATGAAGCA

TCCTGGTAAT

GATAAAGGCT ATG3TTCCCAT AAAAAATGAT TCGTT TGA TTATCGGCCT

AGACAATACA

GATCCTATGA TGGTGATTAC

CGTGAATA-AC

CCACGGGATA GTTTTGTTCA

AATTGATG.CG

GCCGCCTATA CCTACCATGG

ACCAACAGCT

A TTCCAATCA ATCATTACGT TGTGTTTA-AC GTTGGCGGCA TAGATGTCAA

TGTCAAGCAG

CCATCCA'ITC ATTF1MATGC AGGGAAACAG GCCCGTCGAAA GACATAGCGA TAACGATATI' CAAGCAGTTG AAGACAAGTT

GAAATCTGGT

GAT*CGTTAA ATGGAAACAT

TCAAACTGAT

AAAGA.AGGTT TGACTTGGAC

CAATTATGAT

TTTAGTAAI'G AAGGGCGCAG

TATGG'FTGAA

CATCAATTAC GTGTGTCTTT AAA'FrTAGAA GTCTTCCATA CGAACGGTGA

AITITTATAT

GCTGAGGAAA ACGAAATGAC

TTCCATCAAC

ACACAGACCG GCCCGTTGCC

ATCAGTTAAA

*4~ GCTTTATAAA A EF014-4 (SEQ ID NO:S2)

I

AV AGSYLKKTID

KGYVPIKNDY

NEAQNKDSQS FLIMGLDNTI

ERKLGTTRTD

NYQGMQR lEA AYTYDGPTAS VNTVEKLLNI FDGVTKDGPG SIHFOAGKQH

LDGT-KALSYA

SIMCIMDIID SLNGNIQTDV

DSNELTHLVK

YPDSIENVRH QLRVSLNLEK

PDERDQDGYV

NTYIGVPGNT QTGPLPSVKT ENGFIK

AMMVITVNNK

P INI4YVVFNF

RERHSDNDIM

EGLTWTNYDK

FHTNGEFLYQ

TKKITYLSLP

LSFIKLIDAV

RGFRQQEI ,IQ

QQLSFDWRTF

SDYTVQDEAA

RDSFVQ IDAI( CnIDvNVKQA

AVEDKLKSGQ

SNEGRSMVEL

LENEMTS ING EF015-1 (SEQ ID NO:53)

TAATTAAAAA

TTAATAGCTA

AAAAGTTTTG

ATAAATTTAG

AATACTATAG

ATTGCGGTGA

AGATTCATTTI

TGTGTAAAAA

TCTCT'ITITC

ACGACACTAA

ATGATTCAA

AAG LTAAAAA

AAACAGGATG

TAGAAAITTGT

GGGTCTGATG

TCCATAT1T ATATrGCTGTG

AGAATTTATC

TGGGCACGTT

GATATCAGAA

TCAACAATAT

AAAAAAGGAG

ATTTTTTTTC

GTCAAGATAG

AAAACATATT

CGTGTAAAAA

CCAGGGCNAA

TcTAAGGATT

ACATAATAGT

AcA.ATAATCC

ATGGGAAAGA

ATCcATCAAA

AAGATAATAG

CTAGTATCTG

ATTATATTTA

TATTATCTTT

ATI'TAACTCC

GATTGAGCGT

AGGGCAATAT

TCCACATCAA

TATTCCTCAC

CTAA

EF015-2 (SEQ ID NO:54) MX KGDIIVIIFL IAISFSPYFI FFHNNPFNSK SFDDTKYA'JV

KIDGKEIERI

NLDDSKEFIK TYYPSKGQYN TIEVKNGHVR VKKDNSPDQI AVKTGWISEp

GXTSICIPHR

FILEIVQQYS

KDYYIY

EF015-3 (SEQ ID 94 TABLE 1. Nucieotide and Amino Acid Seqeuenccs of E. faecalis Genes.

CAATAATCC ATTTAACTCC AAAAGTI'TG ACGACACTAA ATAAATTTAG ATGATTCAAA AATACTATAG AAGTTAAAAA ATTGCGGTGA AAACACGATC AGATTCATT TAGAAATTGT ATATGCTGTG GTCAAGATAG AGAATTTATC AAAACATATT TGGGCACGTT CGTGTAAAAA GATATCAGAA CCAGGGCNAA TCAACAATAT TCTAAGGATT ATGrGGAAAGA

ATCCATCAAA

AAGATAATAG

CTAGTATCTG

ATTATATTTA

GATTGAGCGT

AGGGCAATAT

TCCAGATCAA

TATTCCTCAC

EF015-4 (SEQ ID NO:56) NNPFNSI( SFDDTKYAVV KIDGKEIERI NLDDSKEFIK TYYPSKGQYN TIEVKNGHVR VKKDNSPD)Ql AVXKTGWvISEP GXTSICIPHR FILEIVQQYS KDYYIY EF01.6-1 (SEQ ID NO:57) 4.

TGACGGTTGC

TTGCTGGTTA

GAAGATCAAT

TTTGTCCCCA

GCCAAACCGG

ATGAAAGAAA

ACGAGCGAGC

CTTGTTTCTT

GGGGTTCAAA

AAATTTGTTA

TTAAAATCTG

TCCCACGAAG

TTTGCTGTGG

GAAkACGTTAC

GTTACAAATA

CCCCGTCCAA

TCTCTTGTAG

GGACACGGAT

TGGGTTTTCA

TTTTTAAACT

CAGAATTACA

GGGCCGAAAA

TAAAAGAAAA

ACGGCTCTTC

AAGACCAAAC

GTCGAATTGA

ATA-A EAAA

GCGTCCGCAA

GAAAAGATGG

ACACAAXAAAT

TAGAAAGGAG

TTTACTCCTA

TAACGAAGAA

AGATAAATCA

TTATGGCATT

AAATCAAACC

AGTTCAAFFC

AAACATTCCA

TGGCTATGAT

ACCTATTA

CGGACTCCTA

AAACTATACT

ATCAGACAAT

CACCCTTAGT

AAACTAA

TTTATCATGA

'TTGCAGGTT

AAACGGATTA

GGCAAAATTG

TCCGTI'ACT

ATTGATCTTA

ACACAACCTT

ACAGCGAGCG

GGCTTCGAAA

TATGACGGCT

ATCGATCGCG

ATTTCTCATG

CAATTAGTCC

AAAATTTCTC

AAAAGAAATA

GTGGTAAAAG

TTATTGGCTT

TCGGCTTTGA

TCCA.ACCGAT

TTTGGAACGG

ACATCACGAA

ACATGCAAGG

GTCAGCCTGA

TTAATGAAGC

TTTACCCCAA

TAGGCTATGA

AAA.AAATCAA

AAAAATGGTT

TTCTTTAGCC

AAA.AAGCAAC

AGATGACTCC

TGTCGACTTA

TG-ATI'CCTCT

CTACACTAAA

CGACCAAGTA

CAAAATTTA

CGTTTTGAAA

TTTCTTAGAT

CTACTATCTT

CAATGAAGAT

TACTGCCT

TGGAGAGGAC

EF016-2 (SEQ ID NO:58)

MMKKKYSLAL

VPMGFQDKSG

SERAEKVQFT

FVKDQTP ILY

AVGVRKSDNQ

LVI CCSLLLF

KIVGFDVDLA

QPYMTNDQVL

DGFNEAFLDL

LVQKINTAFE

AGCCKRKSNE

KAVFKLYGIS

VSLKEKNIAT

KSGRIOGLLI

TLRKDGTLSK

DQWTRINEE(

\JDFQPIDWSM

ASDMQGKILG

ORVYANYYLS

IS QKWFCEDV RuIGILDDSF

KETELQNQTI

VQNGSSGYDG

I-EDNLKNYTI

TNNTKIN

DLIWNGYTKT

FESQPDVLKK

SHVGYDNEDF

EF016-3 (SEQ ID NO:59)

AAGCAAC

GAAGATCAAT

TTGTGCCCA

GCCAAAGCGG

ATGAAAGAAA

ACGAGCGAGC

CTTGTTTCTr

CGGTTCAAA

AAJTGTTA

GGACACGGAT

TGGGTTTTCA

TT=rAAACT

CAGAATTACA

GGCCCAAAA

TAAAAGAAAA

ACGGCTC ITC

AAGACCAAAC

TAACGAAGAA

AGATAAATCA

~TTATGGCATT

AAATCAAACC

AGTTCAAT1'C

AAACATTGCA

TGCCTATGAT

ACCTATI'TA

CGGACTCCTA

AA.ACGGATTA

GCcAAAA'1rG

TCCGTTGACT

ATTGATCTI'A

ACACAACCTT

ACAGCGAGCG

GGCTCrGAAA

TATGACGGCT

ATCGATCGCG

TTATTGGCTT

TCG.GCTTTGA

TCCAACCGAT

TTTGGAACGG

ACATCACGAA

ACATGCAAGG

GTCAGCCTGA

TTAATrCAAGC

=IIACGCCAA

AGATGACTCC

TGTCGACT'A

TGATrTGGTCT

CTACACTAAA

CCGACCAAr-TA

CAAAATITTA

CGTFI'TAAA

F1TCTTAGAT

CTACTATCT

TTAAAATCTG GTCGAATIGA TABLE 1. Nucleotide and Amino Acid Sequences of E. fecalis Genes.

TCCCACGAAG

r=TCTGTGG

GAAACGTTAC

GTTACAAATA

ATAATTTAAA AAACTATACT ATTTCTCATG TAGGCTATGA CAATGAAGAT GCGTCCGCAA ATCAGACAAT CAATTAGTCC AAAAAATCAA TACTGCCTTT GAAAAGATGG CAC-CCTAGT AAAATTTCTC AAAAATGCTT TCGAGAGGAC ACACAAAAAT AAAC EF016-4 (SEQ ID SNE DQWTRINEEK RIIIGLDDSF VPMGFQDKSG KIVGFOVDLA KAVFKLYCIS SERAEKVQFT QPYMTNDQVL VSLKE-KNIAT FVKDQTPILY DGFNEAFLDL KSGRIDGLLI AVGVRKSONQ LVQKINTAFE TLRKDGTLJSI VDFQPIDWSM KETELQNQTI DLIWNGYTKT ASDMQGKILG VQNGSSGYDG FESQPDVLKK rJRVYANYYLS I{EDNLKNYTI SHVGYDNEDF ISQKWFCEIDV TNNTK IN EFC17-1 (SEQ ID NO:61)

TGAGGTGTI'T

CTACTTCTCT

TTACGGGTCG

ACCGCAGCAG

GA.AGCGAAAC

ACTTTTACGA

TTGAATACT

TTGAAGGdT

GGGGTAACAG

TTTCAACAAT

ACGGGCAAAA

GAAGGTTGGG

CAAGCGAATG

AAAAATCTTT

CAAGA-ACAAG

TTAAATACGC

TTGAATTC-TG

TTCGTGCCAA

GATTTAAATC

TTAGGAATTG

ATCAGTGACT

TCACCAGITC

GTTGGTGGCT

AAAAATTCCA

GCAAACGTAA

CAATrGGTTG GTGrGCCGATT

GTCTCTATCG

TTATGAAAAG GGCAACAAAG CGGGCTCTMC A.AGTGTTGGG GGATTGATTC GGAATTATCA A.TGTAATGAG CCAAGTAGGG CAGCATTGGq AACTGAAAAA TTCGAAAAGA TGCAAAATGG CTTGGAAGCG CACAGTGGAC TAAAAAATA TCGTGCTATT CCATTGATGA CCATACCI'G TATrGGCGGT ACCAGCTTT ACTATGGTAC ATCAGCTGAG ATGGCACGAA TAATACTTGG TI TCGCTAGA TAAGGTGGAT TCGAAGGGAA AGAATTAGAT GcAATGCAGc TTTGAAAATT AAAAAGATCT TITGGCAAAT AGCGTTTAGC TAA.AAATGTT CAGGTTTCAC TAATCAAGAA CTAGTGAACC AGAAAAAGCG AAAAAGCGGA GCTAACGATT ATGTTCAAGC AGCTTI'AGCA CTTTTAATAA TCGT~rAGA.A GGACGCCAGT ATATGCTGAT ATAATTTTGG TAAATGGTC'I CTTATGCAAA TAAATATGA" CGGAAGAAGC CCCCCTAGI CTGTCCAAAA TTTAGTCTANI GCGACA.AGTA A

CAAAGGCTGT

AAAGAAACCA

ACGGCAGACG

GAGGGACTTT

GTACACCCCT

AGTAACGG-CG

CCAAAAACAG

GTITGACGGTA

GAAGTAGAGC

TATCCTIAA

TCAACACTTT

TCCTATGTGA

GTCCAAGTAG

GTTGTAAA-AA

CGTGAAATTC

AAGAATGCAC

TTAAATGATG

ACGCAAAAAG

AAAGAGTAT

TTAAGTTCGG

GATAATI'TAG

AAAAGTCGCA

CCAATCGATI

*AATAAGAC CI GAACGTrGGA

*CCTCTTTATC

GGTCCATTAC

CTTTGGCAC

AAAAGCAAGA

TGTCGTTGC

TCTCCITI'A

CCAATGATGG

AGCCAATCAC

CTTCCCCGCA

GCAAATCTAA

TAAGCTATCC

ATGAAGCATT

ACAATGGCGC

AGAATAAkAA rTAAAGAAGT

TTCTGGAGA

CTGGA-ACGTA

GTCGAGCAAT

GCTCAAAAAA

ATTTTGCAGA

GGCAALACCGC

ATACAGAAAA

AAAAT7rAAC

GCGGAGA'-TT

TCTI'AAACTT

TTGATCAGTT

AAACATTACA

AATTA-ACAGA

GTTCAGGCTA

AATCATGGTT

ACAACAGGTA

AATGGATAAT

CGAAAAAGGA

TTTA-AGCTAT

AGCAAATGAT

AGCGTATTAC

AGAAGAGTTA

TATGAGTTAT

TGTCGAAAAA

CTTCACATTA

ETATTGGGAT

CAATACTGGG

AATTGTTGCA

'TATATCCAA

AGCATTATCA

AGCACTTGGC

GGAATTAGGA

TAAAAAAGAA

TGCTAAAAA

AGTCAATGTT

CGACATTGTG

ACTGCAATCA

GCTTCAAGAA

AAAAGCGGAT

AGCACGCTTA

*TI'ACAA.ATCA

EF017-2 (SEQ ID NO:62) MKRATKQ RLSLAAIMVL LLSGCGSVGK ETKKQEQQVL RVGIDSELST ADVSLAMDNT AADVMSQVGE GLFSFDEKGE AKP ALATEKV QPSNDGLSYT FTIRKDAKWS NGEPITANDF EYSWKRTVDP KTASPQAYYF EGLKNYRAIV DGSKS1(EELG VTAIDDHTLE VELSYPMSYF QQLLAVPAFY PLNEAFVEKT GKNYGTSAES TLYNGAFTLE GWDGTNNTNS YVKNKNYWDQ ANVSLDKVDV Q1JVKEVNTGK NLFEGKELDV VXISGEIVAQ EQGNAALXIR EIPGTYYIQL NTQKDLLANK NARRAIALSL NSERLAKNVL NDGSKYALGF VPTGFTNQET QKDFAEELGO TAB3LE 1. Nucleotide and Amino Acid Seqeuenices of E. faecalis Genes.

LNPSEPEKAK ELWQTAXK<EL GIEXAELTIL SSDTENAXKI SEY-VQGALAD NLENLTVNVS PVPFNNRLEK SRSGDFDIVV GGWTPVYADP IDPLNLLQSI( NSNNFG.KWSN KTFDQLLQEA NVTYANKYEE RWKTLQKADQ LVAEEAPLVP LYQLTEARLV ADSVQNLVYG PLGSGYYKSV

SIGIDK

EF017-3 (SEQ ID NO:63) CTGTGG AAGTGTTGGG AAAGAAACCA AAAAGCAAGA ACAACAGGTA

C

TTACGGGTCG

ACCGCAGCAG

GAACGAAAC

ACTTTTACGA

TTTGAATACT

TTTGAAGGGT

GGGGTAACAG

TTTCA-ACAAT

ACGGGCAAAA

GAAGGTTGGG

CAAGCGAATG

AAAAATCTTT

CAAGAACAAG

TTAAATACGC

TTGAATTCTG

TTCGTGCCAA

GATTTAAATC

TTAGGAATTG

ATCAGTGAGT

TCACCAGT'C

GTTGGTGGCT

AAAAATTCCA

GCAAACGTAA

CAATTGGTTG

GTGGCCGATT

GTCTCTATCG

GGATTGATTC

ATGTAATGAG

CAGCATTCGC

TTCGAAAAGA

CTTGGAAGCG

TAAAAAATTA

CCATTGATGA

TATTGGCGGT

ACTATGGTAC

ATGGCACGAA

TTTCGCTAGA

TCGAAGGGAA

GCAATGCAGC

AAAAAGATCT

AGCGTTTAGC

CAGGTTTCAC

CTAGTGAACC

AAAAAGCGGA

ATGTI'CAAGG

CTTTITAATAA

GGACGCCAGT

ATAATTTTGG

CTTATG CAA

CGGAAGAAGC

CTGTCCAAAA

GCGACAAG

GGAATIATCA

CCALAGTAGGG

AACTGAAAAA

TGCAAAATGG

CACAGTGGAC

TCGTGCTATT

CCATACCTTG

ACCAGCTITTT

ATCAGCTGAG

TAATACTTGG

TAAGGTGGAT

AGAATTAGAT

'ITTGAAAATT

TTTGGCAAAT

TAAALAATGTT

TAATCAAGAA

AGAAAAAGCG

GCTAACGATT

AGCTTTAGCA

TCGTTI'AGAA

ATATGCTGAT

TAAATGGTCT

TAAATAT'GAA

CCCCCTAGTT

TAGTCTAT

ACGGCAGACG TGTCGTTr-GC GAGGGACTTT TCTCCT=IGA GTACAGCCCT CCAATGATGG AGTAACGGCC AGCCAATCAC CCAAAAACAG CTTCCCCGCA G ITGACGGTA GCAAXTCTAA GAAGTAGAGC TAAGCTATCC TATCCTTTAA ATGA-AGCATT

TCAACACTTT

TCCTATGTGA

GTCCAAGTAG

GTTGTAAAAA

CGTGAAATTC

AAGAATGCAC

TTAAATGATG

ACGCAAA.AAG

ACAATGGCGC

AGAATAAAAA

TTAAAGAACT

TTTCTGGAGA

CTGCGAACGTA

GTCGACCAAT

GCTCAAAAA

ATTTTGCAGA

AATGGATAAT

CGAAAAAGGA

TTTAAGCTAT

AGCAAATGAT

AGCGTATTAC

AGAAGAGTTA

TATGAGTTAT

TGTCGAAAAA

C'rTCACATTA

ITATI'GGGAT

CAATACTGGG

AATTGTTGCA

TTATATCCAA

AGCATTATCA

AGCACTTGGC

GGAATTAGGA

AAAGAGTTAT GGCAAACGGC TAAAAAAGAA

TTAAGTTCGG

GATAATTTAG

AAAAGTCGCA

CCAATCGATT

AATAAGACCT

GAACGTTGGA

CCTCTIATC

GGTCCATTAG

ATACAGAAALA

AAATTTAAC

GCGGAGAI=

TC-TIAAACTT

ITGATCAGTT

AA.ACATTACA

AATTAACAGA

GTTCAGGCTA

TGCTAAAAAA

AGTCAATGTT

CGACATTGTG

ACTGCAATCA

GCTTCAAGAA

AAAAGCGG3AT

AGCACGCTTA

TITACAAATCA

EF017-4 (SEQ ID NO:64).

C C CCSVGK ETKKQEQQVL RVGIDSELST ADVSLAMNT

AADVMSQVGE

EYSWKRTVDP

QQLLAVPAFY

ANVSLDKVDV

NTQKDLLANK

LNPSEPEKAK

PVPFNNRLEK

NVTYANKYEE

SIGDK

GLFSFDEKGE

KTASPQAYYF

PLNEAFJEKT

QVVKEVNTGK

NARRAIALSL

ELWQTAKKEL

SRSGDFIJIVV

RWKTLQKADQ

AKPALATEKV

EGLKNYRAIV

GKNYGTSAES

NLFEGKELDV

NSERLAKNVL

GIEKAELTIL

GGWTPVYADP

LVAEE-APLVP

QPSNDGLSYT

DGSKSKEIELG

TLYNGAFTLE

VKISGEIVAQ

NDGSKKALGF

SSDTENAKKI

IDFLNLLQSK

LYQLTEARLV

FTIRKDAKWS

VTAIDDHTLE

GWDGTNNWS

EQGNAALKIR

VPTCFTQET

SEYVQGALAD

NSNNFGKWSN

ADS VQNLVYG

NGEPITANDF

VELSYPMSYF

yVKNYWDQ

EIPGTYYIQL

QKDFAEELGD

NLENLTVNVS

KTFDQLLQE.A

PLGSGYYKSV

EF018-1 (SEQ ID TGTCATTACA ACGATACCAA TTTTAATCAT TTATCCATTA CTACAAAAAC ACTTTATCGG CGGTATGATG GCCGGTGCAG TAAAAGAATA AAGAAAGTAG GGAACAATAT OAAAAAAGTT TABLE 1. Nucleotide and Amino Acid Seqeuenccs of E. faecalis Genes.

TTAGCGCTT

AAGAAAGCTA

GCTTGGAACT

TGGACGCGC

GTGGATATTG

GATATGCCTG

TCTTGGGCCT

GTTAACCAAG

AATTACTCTA

GTTATTCGTG

TTTGAAAAAG

ACCACAG-TGG

CCTTTAGAGG

TGGTTAAAAA

GATGATCGGG

GCTGGAACCA

TATATAGCCA

GGCAT'ICAG

ACTCAACTCT

GAAGGAGTTA

AAATTAGAAC

TACTTTAACA

ATGCAACAAT

GATGCAGGAA

GTTAT'ICAA

AAAGCATTCT

GCGGAAAACA

TATTCCTGCC

GCTCAGATGT

TAGATAAGAA

TCC'TT=

AATTTA=IC

ATATCGTGAC

ATTCT'IAAA

ATACC ETTAA

ATACAAAAGC

AAGATCTCTA

TCATCCAACA

GCGATGGTGC

ATAAAAATGG

CATTTAATGA

CAACGTTTGA

GTGGTCAAGG

TTGATGCACC

GTITGGTTAAG

TCACATATTT

CTTATCC-GTA

AA.ACAGACA-A

ACGACCGTGT

CCGGCAAACG

CCCGGAAGC

TGATTCGTGC

TAAAATCAAA

GAGACAAACT

AACGGCGGTC

CTCAATAAG

GAAAGAACCG

AAAACACGTG

CGGCGATGAT

AI'AACTGAA

CGAT-TTACCT

ATGGTATGCC

GGATTATGAA

TAATGCATTA

GATTAAAGAA

AGGACCATTT

TAAATACTAT

TGTTTACCGA

TGAAAAGTG

TGGGAACTTC

AAGTAGCACT

TAATTACATT

AATTGATGAA

CAATCATCAA

TGATGCCTAC

CAATAAACTA

AAAATTAGTC

TCGTGCGAAT

GAAAGATGAT

TAAATGGGAT

TAAGTAA

GTTAGTTTAG

GATCGGTATG

ACCAAGAMfA

ACCACTCCC

TCAAkAATTAA

AAAACTGGAC

AAA.AAATATG

TTAGAGGATG

AGTGGAAATA

GGCAAGCCAG

AAATATCCTC

TTAGACAAAT

GATCGAAATT

GCAGGCAATA

AAACAAGGAA

ACAGAATTTA

TCTGGCCGAA

ACGAAAGATG

CCGGGACAAA

GGAAAAATTG

AACAAAAA.AT

AAAGTACCAA

CCACATTTCG

GAAGCGATTG

AAAGCCI=G

GCAATTGAAA

CGGCCTGTAG

A.ATTAGATGA

AATGGTATAT

AGATTAAAAA

ATGCCATGAT

AAGCAGCALr

ACCCCTA'TT

GAAAAACATA

TCCCAGTAAA

ACGTTI'CAAC

ACATGACCCC

TACAAGACTI'

TAGATAAAGA

TTAGtG'ATGA

ATTATGCAAC

TGAAAAAATC

AACCAACATI'

CGAA-AGATCC

TTTTAACAAA

ATTATTTACC

ATCCCATTAG

TGGAAAGTGC

TAATTGAAAA

A.AACCAAACT

ACAAATCT

AAATAAAATC

CGGTGGGGAA

AAAGACGCCT

TAACTCAGAT

AGACTTAAAT

TTCAACTGGA

GAAAGCAGAT

AATGAAAGTT

TrGGTTACCCT

TGATAAT

ACCAGAAAAT

AATCCGCTTC

CTTAGGTGTT

ATATTTAGAA

TACCTTCACA

CATGCTCGTT

TGGCACACGT

AAATCAAACC

AGCAAAAGTC

ATATGGCGTTr

AGAAGTGAAA

TCGTFI'CCTA

TTTAACGCA-A

TATTAATCCA

AAATACAACC

AGAAGACTAC

TGAGAAAATG

CO. 00** :*o EF018-2 (SEQ ID NO:66) MKKV LCGLLVATAV VSLAACSGGE

KKA.SSDVSIK

VDIEFISGDD

VNQDTFKWYA

FEKVMQQ IKE

WLKTFNDVYR

YIAIDGPSST

EGVTYAYNDQ

MQEWGKCKLV

KATFLKSNKWD

DRYELDEKTP

SKLNAMISSG

LEDGKTYGYP

KYPEMTPMGF

ACNISDDSFT

SGRKPTLNQT

GKIDYLPEVK

PHFVIENINP

AIEKIKSEKM

AWKLDKKKEP

DMPDIVTLTE

NYSNTKADYE

TTVGDGAGPF

DDCATFDEKV

GISGWLSNYI

KLEQTDNDAY

DAGTPEARAN

AENROKLK

TKIKWY INSD

KTGQAALKAD

SGNI PVNDNF

LDKLQDFLGV

KQGNYATMLV

TKDAYXDPA.KV

NKKYGISRFL

EAIETKLNTT

WTALPFGKDV

SWAYSLNDLA

VIREDVYNAL

PLEDKNGKYY

AGTSGQGGNF

TQLFTYLI DE

YFNNDRVNKL

VISMIRAKDD

TTAQ IKKDLN

KKYDPYLMKV

GKPOVSTPEN

DRNLDKEYLE

TEFMXIKSCTR

PGQILTKYGV

KVPMESALTQ

KAFDKSLEDY

EF018-3 (SEQ ID NO:67) CTGTAG CGGTGGGGAA AAGAAACCTA GCTCAGATGT GC I=GGAAGT TAGATAAGAA TGGACGGCGC TGCCTTTCGG GTGGATATTG AA ATTTC GATATCGCCTG ATATCGTGAC TCTTGGGCCT ATTCTT='AAA GTTAACCAAG ATACGTI'TAA AATTACTCTA ATACAAAAGC

CTCAATTAAG

GAAAGAACCG

AAAAGACGTG

CGGCGATGAT

A ITAACTGAA

CGATTTAGCT

ATGGTATGCC

CCATTATGAA

TAATGCATTA

GATITAAAGAA

GATCGGTATI

ACCAAGATTA

ACCACTGCGC

TCAAAATTAA

AAAACTGGAC

AAAAAATATG

TI'AGAGGATG

AGTCCAAATA

GGCAAGCCAG

AAATATCCTG

AAI'AGATGA

AATGGTATAT

AGATTAAAAA

ATIGCCATGAT

AAGCAGCATT

ACCCCTATTT

GAAAAACATA

TCCCAGTAAA

ACC PFCAAC

AGATGACCCC

AAAGACGCCT

TAACTCAGAT

AGACTTAAAT

TTCAAGTGGA

GAAAGCAGAT

AATrCAAAGTT TGG ITACcCT

TGATAATTT'

ACCAGAAAAT

AATGGGCTTC

GTTATTCGTG

TI'TGAAAAAG

AAGATGTCTA

TCATGCAACA

98 TABLE 1. Nucleotide and Amino Acid Seqeuenccs of E. faecalis Genes.

ACCACAGTG

C CTTTAGAGG

TGGTTAAAAA

GATCATGGGG

CMGAACCA

TATATAGCCA

GGCATTTCAG

ACTCAACTGT

GAAGGAGTTA

AAATTAGAAC

TACTI'AACA

ATGCAAGAAT

GATGCAGGAA

GTATTTCAA

AAAGCATTCT

GCGGAAAACA

rGCGATGGTC

ATAAAAATG'G

CATTAATGA

CAACGTITTGA

GTGGTCAAGC

11GATGGACC

GTTGGTTAAG

TCACATATTT

CTTATGCGTA

AAACAGACAA

ACGACCGTGT

GGGGCAAAGG

CGCCGGAAGC

TGATTCGTGC

TAAAATCAAA

GAGACAAACT

AGGACCATTT

TAAATACTAT

TGTTTACCGA

TrGAAAAAGTG

TGGGAACTTC

AAGTAGCACT

TAATTACATT

AA'ITGATGAA

CAATGATCAA

TGATGCCTAC

CAATAAACTA

AAAATTAGTC

TCGTGCGAAT

GAAAGATGAT

TAAATGGGAT

TAAG

TTAGACAA-A-

GATCGAAAT'-

GCAGGCAATA

AAACAAGGAA

ACAGAATTA

TCTGGCCGAA

ACGAAAGATG

CCGCGACAAA

GGAAAAATTG

TACAAGACTT

TAGATAAAGA

TTAGTMATGA

ATTATCCAAC

TGAAAAAATC

AACCAACAT

CGAA-AGATCC

TT'rAACAAA

ATTA=TACC

CTTAGGTGTT

ATA'I=ACAA

TAGCTTCACA

CATGCTCGTT

TGGCACACGT

AAATCAAACC

AGCAAAACTC

ATATGGCGTT

AGAAGTGAAA

TCG TrCCTA

TTTAACGCAA

TATTAATCCA

AAATACAACC

AGAAGACTAC

TGAGAAAATG

AACAAAAAAT ATGGCATT-AG AAAGTACCAA TGGAAACT-GC CCACATTT~CG TAATTAAAA GAAGCGAT7G AAACCAAACT AAACCCTTTG ACAAATCTTT GCAATTGAAA AAATAAAATC EF018-4 (SEQ ID NO:68)

C

C*

C.

*CSGGE

KKASSDVSI(

VDIEFISGOD

VNQDTFKWYA

FEKVMQQIKE

WLKTFNDVYR

YIAIDGPSST

EGVTYAYNDQ

MQEWGKGKLV

KAFLKSNKWD

DRYELDEKTP

SKLNAMI SSG

LEDGKTYGYP

KYPEMTPMGF

AGNISDDSFT

SGRKPTLNQT

GKIDYLPEVK

PHFVIENINP

AIEKIKSEQ4

AWKLDKKKEP

OMPD IVTLTE

NYSNTKADYE

TTJGDGAGPF

DUGATFDEKV

GISGWLSNYI

KLEQTDNDAY

DAGTPEARAN

AENRDKLX

TK IKWY INSO

KTGQAALKAD

SGNIPVNDNF

LDKLQDFLGV

KQGNYATMLV

TKDAKDPAXV

NKKYCISRFL

EAIETKLN--,

WTALPFGKDV

SWAYSLNDLA

VIREDVYNAL

PLEDKNGKYY

AGTSGQGGNF

TQLFTYLIDE

YFNNDRVNKL

VISMIRAODD

TTAQIKKDLN

KKYDPYLMKV

GKPDVSTPEN

DRNLDKEYLE

TEFMKKSGTR

PGQILTKYGV

KVPMESALTQ

KAFDKSLEDY

EF019-1. (SEQ ID NO:69)

TAAAGGAGTT

CTTGGTTCAT

AAAACACATG

CGGATTATrG

CAATGGACAG

CCCACTATT

TTAATCAGTT

CCAACTTATG

GCCACTGTTT

ACCAAAGGCG

TGGGTA.ACCA

TATCAGGACT

GCGGATTGGA

CTTGTAAACA

GCTGTGAAAA

CCTArI'GCGA ACACAATGAA ACTT=AAAA

TCTTACTCGC

AAGTAACAGA

CGAGTTA=T

F1GGACAAGG

CCTATGACTT

CATCTGCTCT

TAGTCAAAAA

TAGATAAAAA

TCCAAGAATA

ACAACCAAGT

TAGGCCTCCA

ATCAAGTTI'C

GCGATGAATC

ATAACCAAGT

ATACTCAAAT

AGCTTGTGGT

TACCTTAGGC

AGAAGATTAT

CAGCATTCAA

GCCATATGAA

AGTTGAAGGC

CGGCGAAAAT

AGAACAAGCG

TCTTCGCAAA

CTTTATGGTT

AGTI'CCAAAA

T'ITAGAAAAA

AGCACCTCTT

TCATACCTAT

TGTTGAAGAT

AAGACGGTCC TAATTGGTAC AATACGAATA AAGA.AGCCAA AATAAAGTAA CCGTCCCCGC CTAGTTGCAT TAG GAGAAAA

GATTATTIAG

GCGGTTCTAA

GGTAAATACA

GTCACCTCGC

AAAAAAGTGT

AAAGATGCTG

AGCGATAATC

TTAGTGGAAG

TTAGCTGAGC

TTCCA-AGAAG

CGAAAGAATT

AATT~TGAACC

AAGAATACAG

GTATCAAT

TAGAAGATTA

GCAAATCTGC

GCTCAAGCGG

AAATTCTAA

TTGACGCAGA

CAATTTGGAA

AACCCTTCTT

CAACGCTGAC

GAAACCCAAA

ACCAGTGGCA

GAAAGATGTC

TGACTTATTA

TAAAATTGCG

GGAAGATATT

TGATACCTI'A

GGCAGTCTTA

AACCGTGCTC

AAACGCTACT

CCACATTTTC

GAACTTACCT

GATAAAAAAA GTAGTTCGGTT ATACAACGGA GTAAAAAAAG CGCTCT-TAAA 'ITAA EF019-2 ((SEQ ID MKLLKK TLIGTITLLL CSFLIJAACGN TNKEANNADK THFJTDTLGN KV'IVPAKPKR IIASYLEDYL VALGEKPVAQ WTVGQGSIQD YLAKELKDVP TISYDLPYEA VLKFEPDLLL 99 TABLE 1. Nucleotide and Amino Acid Seqeucnccs of Efaecalis Genes.

ISSSALVEGG KYKEYSKIAP TYVVKNqGENV TWRDQLEDIA TVLDIKKEQAK KVLEDYDTLT KGVQEYLGKK DAGKSAAVLW VTNNQVFMVS DNRSSGT\JLY QDLGLQVPKL 'JEEISKNATA DWNQVSLEKL AELDADHIFL VNSDESAPLF QE-AIWKNLPA VXNNQVHTYD KKSSWLYNGP IANTQIVEDV KKALLN EF019-3 (SEQ ID NO:71) TTGTGGT AATACGAATA AAGAAGCCAA CAACGCTGAC

AAAACACATG

CGGATTATI'C

CAATGGACAG

CCCACTAT

TTAATCAGTT

CCAACTTATG

GCCACTGTTT

ACCAAAGGCG

TGGGTAACCA

TATCAGGACT

CCCAT'rGGA

CTTGTAAACA

GCTGTGAAAA

CCTA'ITGCGA

AAGTAACAGA

CGAGTTATTT

TTGGACAAGG

CCTATGACTT

CATCTGCTCT

TAGTCAAAAA

TAGATAAAAA

TCCAAGAATA

ACAACCAAGT

TAGGCCTCCA

ATCAAGTTTC

GCGATGAATC

ATAACCAAGT

ATACTCAAAT

TACCTTAGGC

AGAAGATTAT

CAGCATTCAA

GCCATATGA.A

AGTTIGAkAGGC

CGGCGAAAAT

AGAACAAGCG

TCTTGGCAAA

CTTTATGGTT

AGTTCCAAAA

TTTAGAAAAA

AGCACCTCTT

TCATACCTAT

TGTTGAAGAT

AATAAAGTAA

CTAGTTGCAT

G-ATTATTTAG

GCGGTTCTAA

GGTAAATACA

GTCACCTGGC

AAAAAAGTGT

AAAGATGCTG

AGCGATAATC

TTAGTIGGAAG

TTAGCTGAGC

TTCCAAGAAG

GATAAAAAAA

GTAAAAAA.AG

CCGTCCCCGC

TAGGAGAAAA

CGAAAGAATT

AATTTGAACC

AAGAATACAG

TAGAAGATTA

GCAAATCTGC

GCTCAAGCGG

AAATTTCTALA

TTGACGCAGA

CAAT=~GAA

GTACGTTGGT'

GAAACCCAAA

ACCAGTGGCA

CAAAGATGTC

TGACTTATTA

TAAAATTGCG

GGA-AGATATT

TGATACCTTA

GGCAGTCTTA

AACCGTGCTC

AAACGCTACT

CCACATTTTC

GAACTTACCT

ATACAACGGA

S

S

CGCTCETAAA T EF019-4 (SEQ ID NO:72) CGN TNKEANNADK THEVTDTLGN KV'IVPAKPKR IIASYLEDYL VALGEKPVAQ WTVGQGSIQD YLAKELKDVP TISYDLPYEA ISSSALVEGG KYKEYSKIAP TYVVKNGENV TWRDQLEDIA TVLDKKEQAK KGVQEYLGKK DAGKSAAVLW VTNNQVFMVS DNRSSGTVLY QDLGLQVPKL DWNQVSLEKL AELDADHIFL VNSDESAPLF QEAIWKNLPA VKNNQVHTYD IANTQIVEOV KKALLN

VLKFEPDLLL

KVLEDYDTLT

VEEISKNATA

KK S SWLYNG P EF020-1 (SEQ ID NO:73) 5* 5 .5 *5

TGAGGAGATG

ACATTAACTG

ATAATGAAAG

TATAAAGATA

ACTAGCGCAT

AGTAAAGTAA

GGGAAATrrA

AGAAAATGAA

CATGTAATGG

ATTCTTCATA

AAGACACTAA

TGGAGTAT

CCTGrATGA

AATAA

AAAGGTAGTT

TrCTAAATTA

TGGTGATGAA

TCGTTATTTG

TTATTATTAT

TATGAAAGCT

TCAAT=TGT

GATAAAACAG

TATTCAGA.AG

GCTGATGTT'

GATGAAGATA

ACTGGAAACT

TGATGGTTGT

GTGAAGA.A T

ATIGGT=P'AG

GGGTTCCTCT

AGCGATTAGA

ATIGAAGTAGT

TGCAGTC'ITC

TAAAA.ATTCT

TTTTTAATA

TAAAGATGAA

TAGTACTAAA

GTATAAATCA

EF020-2 (SEQ ID NO:74) MXKVVS ILLMVVAVFT LTACNGSKLD KTGEEFKNSI MKDSSYGDEY SEDCFSFLIY KDKDTNRYLA DVWVPVKDET SALEYFYYYD EDKRLDSTKS KVTFDDMKAS GNYEVVYKSG

KFK

EF020-3 (SEQ ID ATGTAATGG TTCTAAATA GATAAAACAG GTGAAGAATT TAAAAATTCT TABLE 1. Nucleotide and Amino Acid Scqeuences of Efaeca/is Genes.

ATAATGAAAG ATTC'FrCATA TGGTGATGAA TATTCAGAAC TATA.AAGATA AAGACACTAA TCGTTA'FFG GCTGATGC=1 ACTAGCGCAT TGGAGTATI TTATTATrAT GATGAAGATA AGTAAAGTAA CCTTTGATGA TATGAAAGCT AGTGGAAACT GGGAAA=TA AA ATGGTTrAG

GGGTTCCTGT

AGCGATTAGA

ATGAAGTAGT

'FFFITAATA

TAAAGATGAA

TAGTACTAAA

GTATAAATCA

EF020-4 (SEQ ID NO:76) CNGSKLO KTGEEFKNSI I MK(DSSYGDEY SEIDGFSFLIY KDKDTNRYLA DVWVPVKDET SALEYFYYYD EDKRLDSTI(S KVTFDDK(AS GNYEWVYKSG

KFK

EF021-1 (SEQ ID NO:77) a.

a 4* a a a a a a a

TAGTTGTTTA

TTATTCGT

GGCAAAGGCA

ATCATTACAG

TTGCAACT

CAATCGAA E,

AACACAATCT

AACCCTGATA

TCTGCAACAT

ACAAAAACGA

CAAGCTGGTT

GTTGATACCA

GCAATGTACC

CTCTTCCAAG

GGCGTTGACC

AACTTCACGT

CGTGCGTTAG

GGCGTTGACT

AAAGATAAAG

AATACATTAA

TTAGTTTGAT

AAACCGCTGA

ATACAGGCGG

GGGGTAAAGA

ATGCAGCTGA

TTGGTATTGG

CAAAC'TI'GT

TTAGAGATAA

ACAA.AGTCGG

TTGAAAAAGG

AATATGCGGC

AAAACGGCGT

AAGCAAAAGA

GCGATCAAGA

TAACTTCAAC

AAGACAAATI

TAACAGACGG

TAATCTCAGG

ACTATTTTA

TGCATTAGOT

AAGCGGCGGT

CGTGGATGAC

ACATGATTTA

CTATACAACC

CTACTTGCTA

TTTAATCGAT

TGA.AGCAGCT

TTTTGTTGGT

TGTGGCTGAT

TTCATTGrCT

TGATATCATC

CTTGAATGAA

TGCTGATGGC

GCITAAAGGT

CCCTGGTGGC

CTATTTAAAC

TGACGTAAAA

GGAGGCTTTA CAGAAATGA-A TTATCAGTTT CACTI'GAGC GGCAAAGGGG ATGCAGCGCA AAGTCGTTCA ACCAATCTTC CCAGAAGGTT CAAAAGC.GTA AATATTGACC AAGCGGTATC AAAGATGCAA TTTCTTCTGC GATCAAATCG ATGGCAAAA.A TACT1'AGCCG GTGTTGCTGC GGTGAAGA-AG GGGTCGTAAT

AAAAGCAAAA

ATGTGGTGGT

TAGTGCTGTA

TTGGGAAGGA

TGCATATATT

AAGTAAATTC

AGCAGATGCC

GAATGTCGTT

TGCAAATGAA

TGACCGTTTC

AGAAATTACT

TTTAGCTGCT

TGGACAAGGG

'PTGGGTAATC

CAAAGAAGAC

TATTGCCAAC

AAAAGATGGT

TAAAACAGCA

GCTGCGA-AAG

GATCCTCCCA

TTCCATGCTT

TCAGGTTCTG

AAGTACAAAA

GTCGG3CACAG

GAACATTTAG

GACAAAACAA

GTCCCAGAAA

AATTIAGGTA-A

AAGGGAA.AGC

CTGGT'GCGAC

GCGACAAAGT

CA.AAAGACGG

CGGTTAAGA

=I'GGATT

AAGAAG^C-GT

a AACCACAATA A EF021-2 (SEQ ID NO:78) MKKAXL FGFSLIALGL SVSLAACGGG KGKTAESGGG KGDAAHSAVI ITDTGGVDDK SFNQSSWEGL QAWGKEHDLP EGSKGYAY IQ SNDAADYTTN TIFGIGYLLK DAISSAADAN PDTNFVLIDD QIDGKKNVVS ATFRDNE-AAY KTNKVGFVGG EEGVVIDRFQ AGFEKGVADA AKELGKEITV DTKYAASFAD MYQNGVDIIF HASGATG-QGV FQE.AKDLNES GSGDKVWVIG VDRDQDADGI( FTLTSTLKGv GTAVQDIANR ALEDKFPGGE HLVYGLKDGG VDLTDGYLND DKVISGDVyKV PEKPE

IDQAVSSKFN

LAG VAAANET

PAKGKALAAA

YKTKDGKEDN

KTKEAVKTAK

EF021-3 (SEQ ID NO:79)

ATGTGGTGGT

GGCAAAGGCA AAACCGCTGA AAGCGGCGGT GGCAAAGGGG ATGCAGCGCA ATCATTACAG ATACACGGCGG CGTGGATGAC AAGTCGarrCA ACCAATCITC TTGCAAGCTT GCGGTAAAGA ACATGATTTA CCAGAAGGCTI CAAAAGCGTA CAATCGAATG ATGCAGCTGA CTATACAACC AATATTGACC AAGCGGTAI'C

TAGTGCTGTA

TTIGGGAAGGA

TCCATATATT

AAGTAAALTC

101 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. fiacalis Genes.

AACACAATCT

AACCCTGATA

TCTGCAACAT

ACAAAAACGA

CAAGCTGGTT

GTTGATACGA

GCAATGTACC

GTCTTCCAAG

GGCGTI'GACC

AACTTCACGT

CGTGCGTTAG

GGCGITGACT

AAAGATAAAG

TTGGTATTGG

CAAACTTTGT

TTAGAGATAA

ACAAAGTCGG

TTGAAAAAGG

AATATGCGGC

AAAACGGCGT

AAGCAAAAGA

GCGATCAAGA

TAACTTCAAC

AAGACAAATT

TAACAGACGG

TAATCTCAGG

CTACTTGCTA

TTTAATCGAT

TGAAGCAGCT

TTTFIGTTGGT

TGTGGCTGAT

TI'CATTTGCT

TGATATCATC

CTTGAATGAA

TGCTGATGGC

GCTTAAAGGT

CCCMTGGC

CTA=TAAAC

TGACGTAAAA

AAAGATGCAA

GATCAAATCG

TACTTAGCCG

GGTGA.AGAAG

GCTGCGAAAG

GATCCTGCCA

TTCCATGCTT

TCAGGTTCTG

AAGTACAAAA

GTCGGCACAG

GAACATTrAG 6ACAAAACA.A

GTCCCAGAAA

TTTC'ITCTG~C

ATGGCAAAAA

GTCTTGCTC

GGCGTCGTAAT

AkATTAGGTAA A.AGGGAAkAGC

CTGGTGCGAC

GCGACAAAGT

CAAAAGACGG

CGGTTCAAGA

TTTATGGATT

AAGAAGCTGT

AACCAGAA

AG CAGAT C C

GAATGTCGTT

TGCAAATGAA

TGACCGTTTC

AGAAATI'ACT

TTTAGCTGCT

TGGACAAGGG

TTGGGTAATC

CAAAGAAGAC

TATTGCCAAC

AAAAGATGGT

TAAAACAGCA

EF021-4 (SEQ ID 0 0 0 0* 00 @0 0 0 0 0@ 0* 0 0 *00 00 0 0 000 0 000000 0 00*0 0 .000 0 000 0 0000 0000 0 0* 0 00 CGGG KGKTAESGGG KGDAAHSAVI ITDTGGVDDK SFNQSSWEGL QAWGKEHDLP TIFGIGYLL( DAISSAADAN PDTNFVLIDD KTNKVGFVGG EEGVVIDRFQ AGFE-KGVADA MYQNGVDIIF HASGATOQGV FQEAKDLNES FTLTSTLKGV GTAVQDIANR ALEDKFPGGE DKVISGDVKV PEKPE EGSKGYAYI Q

QIDGKKNVVS

AKELGK(EII'V

GSGDKVWVIG

HLVYGLKDGG

SNIDAADYTTN

ATFRDNEAAY

DTKYAASFAD

VDRDQDADGK

VDLTDGYLND

IDQAVSSKFN

LAG VAAANET

PAKGKALAAA

YKTKDGKEDN

KTKEAVKTA(

EF7022-1 (SEQ ID NO:81)

TAAGAGCATA

ACAATGGT

AAAAAGAAAC

ACGCTCAACA

GAAGGGTTAT

CCGATGA ITT

AGTAACGATG

CCTAAAAACG

ATCTCAGCGG

TTA.AAGGrIGA

TTITTTCCCC

GATAAAGTCG

TGGCAACTAG

AATTATACAG

GATGTGGCTA

TCGTATCCAA

ACGCCGCrrG

CTAGTCAATA

TTTGTGGCGA

TATAACAAAG

GTTAACG LTG

CAAGGCTCGC

GAAGCTGCAT

CCAGACTATC

TATCAGAACC

CCAGAAAAA.A

AAAAAATGAA

GTATTTTATT

AGAAAAATAC

CCTCTGTATT

ATAGT IAGA

CAGAAGATGG

ATCCTGTCAC

GCTTTGT'ITA

GGAAATTAGC

CGCTCAAAGA

AAAATCNAAA

TCTATAATGG

CAAAA.AATAA

TTATCAAAGA

CACTAAGTGG

CAGCGACAAT

CAAACGAAAA

ATATTA'ITGC

ATCCCACAAC

AAAAAGCGCA

AArTrGATGGT

TACAAGAATT

TGAACTI'TGG

AAGACCCTAT

CTGTCTATGA

GATGGGCGAC

GAGTTATAGG

GGTAGGATTT

CAAAGAAGCC

ATTGGATTTTI

TGAACA.AGAC

AAAAACCTAC

AGCACATGAT

TAGCTTCCTC

ACCCAATGAA

GCCAAAACCG

AGTAGTCGAA

TCCGTTCGTG

TCGCTATTGG

AACATCTACC

TGAACTGGCG

GAACTATTTG

CCTGCGTAAA

AGATGGTTCT

GGGTCTCGAT

AAGTTATTGG

AACAGATGAT

GTTTCCTGGT

GCGAGAAAGT

TTCTACCCTG

CAAATI'A?'A

ACTGATTGCA

AGAAAGAAGA

TTAGCTGGGT

GTTCAACTGA

CCAGATGCTA

CAATTGGTAC

ACGATTTCTT

TTTGAATATG

ATCGTTGAA-A

CTAGGTGTCA

TACTTTACGT

CAATTTGGTG

GTAAAAGAT

GATCACCAGA

GCATTGAATC

CAACAGAATA

CGCTTAAATC

GCATTGGCTT

AAAGCGCTAC

T'FTCGTCAAG

AAAAAAGCAC

GGTTCTTACA

TTGACAATAG3

GACTATGATT

ATGACTTrAT

GATGAAGCAG

GCTGAAAAAG

TGAAAAAGTA

GTACCAATAA

TGTCACCCTC

TTGTCCAAAC

CAGCCGTAGC

TGAGAAAAGA

CTTGGAAAAA

CAATrCAAAA

CAGCTGTGGA

CC=TTTAGC

CGGACTATGG

GGCAGCAAAC

ACGTGCGCTC

TTTTIAAGA

AAAATAATAC

AAAAACGGNA

TAGGAATAGA

ATGGTGCGAT

AAGCAGGTAA

AAGCAGAATT

AAAAAATTGG

AGCTAACCGC

TATTCITrAAT

ACAAGGGCAA

CCACAACCTA

AAGTGATTGA

'rTTAAAAATC

AAATGAAAAT

GGAATTAACA

TGCAGCGTTT

AAAAGCATG

AGCGGTTTG

AATGATTGAT

TGGTGCAGAA

TGATTATACA

TTTTCCGACA

AACTGCTAGT

AAAGATGGAC

AGACATTATC

TGGACAATTA

GTTGTATCAT

AGGGCAAGCN

TAAAGAAAAT

TACGGAAGGC

TTTAATGGTT

AGGAGAAAAG

TGAAAGT ITG

A'ITGCCGACT

TTACTGGACA

TGATCGCAAT

TGCCTTAGAG

AACGACTGCT

102 TABLE 1. Nucleotidc and Amino Acid Seqeuences of E. .aecalis Genes.

GGCATCATTC CACTTAGCCA AAATGALACAA ACAGTCCTGC AAAATGATAA AGTCAALAGGC FIGSAATTTTC ATACCT TCrG CGCTCCATTA ACGTTAAAAA ATGTTTATAA GGAAAAATAA EF022-2 (SEQ ID NO:82) MKKYLK IT MVC ILLVGFL AGCTNXNENK KKQKNTKEAV QLMSPSEL-T

LNTSVLLDFP

NODPVTAHDF

KVTLKEPKPY

QL.AKNNRYWD

YPTATMNYLR

VANPTTGLDF

GSLQELFPGL

QN'PVYDKLLD

NFHTFGAPLT

DAIVQTAAPE

EYAWKXMIDP

FTSLLAFPTF

HQNVRSDIIN

LNQKRXGQAT

RQEAGNLMVY

TIELTALPTE

EAATTYALEP

LKNVYKEK

GLYSLDEQDQ

KNGFVYSFL I

FPQNXKVVEQ

YTVIKETSTA

PLANENLRKA

NKEKAQSYWK

AALNFGRESD

EKRWATL IAA

LVPAVAKALP

\TETIQNGAEI

FGADYGTASO

LNLFEDGQLD

LALD IDKENL

KAQAELGEKV

YDLFLIYWTP

EKEV'IETTAG

MISEDGKflIT

SAGKLAPNEL

XVVY.NGPFVV

VATLSGELAQ

VNNI IADGSK

NVE-LMVTDDG

DYQDP ISTLM

MIPLSQNEQT

I SLRKE-AVWS

GVTAVIDDYTL

KDWQQTKMDW

QNKNNTLYHS

ALHGAITEGF

SYKKIGESLQ

TLYKGNDRNY

VLQNDKVKGL

00 0 @0 0 4ss Vs 6 0 06000 00000 0*00 00 0 EF022-3 (SEQ ID NO:83) GT GTACCAATAA AAATGAAAAT

AAAAAGAAAC

ACGCTCAACA

GAAGGGTTA.T

CCGATGATTT

AGTAACGATG

CCTAAAAACG

ATCTCACCGG

TTAAAGrGTGA

TTT'ITCCCGC

GATAAAGTCG

TGGCAACTAG

AA ETATACAG

GATIGTCCCTA

TCGTATCCA-A

ACGCCGCTTG

CTAGTCAATA

TTTGTGGCGA

TATAACAAAG

GTTAACCTTG

CAAGGCTCGC

GAAGCTGCAT

CCAGACTATC

TATCAGAACC

CCAGAAAAAA

GGCATGATTC

TTCA.ATTTTC

AGAAAAATAC

CCTCTGTATT

ATAGTI'TAGA

CAGAAGATGG

ATCCTGTCAC

GCTTTGTTTA

GGAAATTAC

CGCTCAAAGA

AAAATCNAAA

TCTATAATGG

CAAAAAATAA

TTATCAAAGA

CACTAAGTGG

CAGCGACAAT

CAAACGAAAA

ATAT'ATT'CC

ATCCCACAAC

AAAAAGCGCA

AATTCOATGGT

TACAAGAATT

TGAACTT1'GG

AAGACCCTAT

CTGTCTATGA

GATGGGCGAC

CACTTAGCCA

ATACCTTTGG

CAAAGAAGCC

ATTGGNFI

5 Fr

TGAACAAGAC

AAAAACCTAC

AGCACATGAT

TAGC'ITCCTC

AC CCAATGAA

GCCAAAACCG

AGTAGTCGAA

TCCGTTCGTG

TCGCTATTGG

AACATCTACC

TGAACTGGCG

GAACTATI'T

CCTGCGTAAA

AGATGCTTCT

GGGTCTCGAT

AAGTTATTGG

AACAGATGAT

GTTTCCTGGT

GCGAGAAAGT

'TCTACCCTG

CAAATTATTA

ACTGATTGCA

AAATGAACAA

CGCTCCATTA

GTTCAACTGA

CCAGATGCTA

CAATTGTAC

AC-ATrC T

TTTGAATATG

ATCGI'GAAA

CTAGGTGTdA

TACTTTACGT

CAATTTGGTG

GTAAAAGATT

GATCACCAGA

GCATTGAATC

CAACAGAATA

CGCTTAAATC

GCATTGGCTT

AAAGCGCTAC

TTTCGTCAAC

AAAAAAGCAC

GGTTCTACA

TTGACAATAG

GACTATGATT

ATGACTTTAT

GATGAAGCAG

GCTGAAAAAG

ACAGTCCTGC

ACGTTAAAAA

TGTCACCCTC

TTGTCCAAAC

CACCCTAC

TGAGAAAAGA

CT-GGAAAAA

CAATTCAAAA

CAGCTGTG.GA

CCTTGTTAGC

CGGACTATGG

GGCAGCAA.AC

ACGTGCGCTC

TIITGAAGA

AAAATAATAC

AAAAACGGNA

TAGGAATAGA

ATGGTGCGAT

AAGCAGGTAA

AAGCAGAAT

AAAAAATTGG

AGCTAACCC

TATTCTTAAT

ACAAGGC-CAA

CCACAACCTA

AAGTGAT'rGA

AAAATGATAA

ATG=TATAA

GGAATAACA

TGCAGCGTI'

AAAAGCATTG

AGCGGTTTGG

AATGATTGAT

TGGTGCAGAA

TGATTATACA

TTTCCGACA

AACTGCTAGT

AAAGATGGAC

AGACATTATC

TGGACAATrA

GTI'GTATCAT

AGGGCAAGCN

TAAAGAAAAT

TACGGAAGGC

TTTAATGGTT

AGGAGAAAAG

TGAAAGTTTG

ATTGCCGACT

TTACTGGACA

TGATCGCAAT

TCCTTAGAG

AACGACTGCT

AGTCAAAGGC

GGAAAAA

EF022-4 (SEQ ID NO:84) CTNKNENX KKQKNTKEAV QLMSPSELTT LNTSVLLDFP DAIVQTAAFE CLYSLDEQDQ LVPAVAKALP M7ISEDGKTYT ISLRKEAVWS NDDPVTAHDF EYAWKKI'.IDP KNGFVYSFLI VETIQNGAEI SAGKLAPNEL GVTAVDDYTL 103 TABLE 1. Nucleoride and Amino Acid Seqeuences of E. faecalis Genes.

KVTLKEPKPY

QLA-KNNRYWD

YPTATMNYLR

VANPTTGLDF

GSLQELFPGL

QNPVYDKLLD

NFHTFGAPLT

FTSLLAFPTF

HQNVRSDIIN

LNQKRXGQAT

RQEAGNLMWY

TIELTALPTE

EAATTYALEP

LKNVYKEK

FPQNXKVVEQ

YTVIKETSTA

PLANENLRKA

NKEKAQSYWiJ(

AALNFGRESD

EKRWATL IAA

FGADYGTASD

LNLFEDGQLD

LALGIDRENL

KAQAELGEKV

YDLFL IYWTP

EKEVIETTAG

KVVYNGPFWV

VATLSGELAQ

VNNI IADGSK

.WELMVTDDG

DYQDPISTLM

MIPLSQNEQT

KDWQQTKMIDW

QNKNNTLYIs

ALHGAITEGF

SYXKIGESLQ

TLYXCNDRNY

VLQNIDKVKGL

EF023-1 (SEQ ID

C

TAAAATGGAG

GCTTTAACGG

CAAA.AAATTG

GATACGACCT

GAAGATGAAT

GTTTATACAT

AACGACTTTG

GCTTTATTTT

AATACTCTTG

ACCCCTTATT

ATCAAAGAAC

TTTAAATTGA

ACGTATTGGG

CCAACGACAG

GGTGAATITA

ACATAC'TIT

AACAATATTC

TTGCAAGATC

GATGcGAACAG

GCGAAAGCAA

TTAGAATTTT

CAATTGGAAG

CGTGTTGATC

TATCGTGATC

ACGTTCAAGA

GATCAAGAGG

ACGGTTTTAG

GATCTCTATT

GGATCGGTAT

CTTGTCAGGC

CAATTAGTTC

CGACTTTAGT

TAGAGTTGGG

TTAAGATTAG

TTTATGCATG

TTGATGTCAT

GGOTTAAGGC

TGAA.ATCATT

AAGGGGATAA

AAGAATGGG'A

AMTCAAAA

CGGTGAA=~

TCCTGGTTA

TAAAAATCAA

GTAAAGCGTI'

AATCAACGGC

ATTTCACAAA

AAGAATTCTG

TAACAGATGA

AAAATCTAGA

GTGATCAAAC

CA?1'AACAGT

GTGATACGTA

CTCCTTTAAA

CACCAATCTA

GGCATTCATT

GAAGAAkATTA AAAATGTTAG GGGAACGGGA A ACTCGGCTG TGAAGCGGCT ATTTCGACAA CATGAATCAA CTTTATGAAG GGTCGCTGCC GAAGAACCAG AGAAGATGCC AAATGGTCGA GCAACAAGTT CCTTCCCCTA TAAAAATGCT AAGGAAATTG GCTAGATGAT AAAACGTTAG ACTTTLCG=T CCTC.TTTTGT ATATGCTACT GATGCAGAAC TAATGCCTCT TCTGATGACT AGTTAAATTA ACAGAAGCGA GrI-GACTCG AATGAATrGG TGTTGATAAT CCAGCCT=TC CAGCGTTCGT GATGGAAAAG GGCACAAGCT TTTGATAAAG TACAGATCALA GTAATTCCGC ACTAGCTGCT AAGAAAAATA GGAAAAAGGG AAAAAAGAAA TACAGACAGC GCCAAAAAAG TGGATTAGAA GTGAATGI'TA GAGAGACTAT GATITAGAAT TATGCGCATC TTTACTTCGG TGATCAATTA ATTCAAGAAA TGACTTTGCT CAAGCACAAG CAATCGAAGC AT= CTGTAT TGGACCCACG TACAGT= AA

GATGCGTCGG

ATAGTAACAA

TGGAACCACA

G.AGTd.'ATG.T

CGATTTCTGA

ATGATGATCC

AATCAGGATC

CTTTAGAACG

AAATAAC=T

'TCCACAAAA

ATTI'ATTTA

GGACCTACGA

AAGTTTCACT

ATGTAGTGA

TTTCA.ATTCC

AAAATCCGGC

AAAGTTTI'T

CGGGACAAAC

ACTACTTAAC

7MTGGCTGGA

CTGCTGAGTT

CTCAAGTTCC

TATCTGGTTG

ATAGTACCFI'

CTAGAACAAC

ATATI'TTGGT

TAGCTAATCA

AATGGGCTTA

G'ITGCTTTTA

AGCAGCGGAA

CACAGCGGGG

TTIAGGTAA.A

AGATGAAACC

AGTAACAGCA

GATTCATCAA

CGCAGATGTG

AGAACGGCCC

TGAAAAATAT

TAATGGTCCT

AAAAAATGAT

AATI'AAGAGC

TAAGCTAAGT

TCAATTCGTC

=I'AGCGAAC

AAAAGAAGTC

GATTGCGCCA

CTACGATACA

TAAAATCAAA

TT1'CCAATITT

TTTACTATT

GGGAACCGAT

GGGCGGCGTA

ACATGCGGCT

GAATCAGGAA

AAAAATCAAG

TGTI'AACTAA

EF'023-2 (SEQ ID 00:86) MKKLK MLGCVGLLLA LTACQAGTr.N SADSNKAAEQ KIAISSEAAI STNEPHTAGD TTSTLVM4NQV

DFVYAWQQVA

PYLKSLLSFP

YWDAEI{VKLT

YFLKMNSVRD

GTDFTKLAAX

LEENLDGLEV

FKSDTYDQLI

LYWHSF'GPTY

YEGLYVLGKE

SPKSGS IHQA

VLFPQNEKYI

EAKVSVIKSP

GKENPALANN

XNNYLTYDTA

NVTQVPFTIR

QETRTTHAAD

SLKWAYVN

DELELGVAAE

LFFDVIKNAI(

KEQGDKYATD

TTAVNLFDSN

NIRKALAQAF

KAKEFWEKGK

VDRDQTRDYD

QEARLNDFAQ

EPAISEDETV

EIALEGADVN

AEHLIYNGPF

ELDVVNI(LSG

DKESFVKEVL

KE IGLDKIKL

LELSGWGTDY

AQDILVNQET

YTFKI REDAX

TLGVKALDDK

KLE:WDNASS

EFI PGYVDNP

QDQSTATDQV

EFLTDDTDSA

ROPLTVMRIF

VLAPIYNRSI

WSNDDPVTAN

TLEITLERPT

DDWTYEKNDT

AFLS IPQFVT

IPPGQTIAPD

KKAAEFFQFQ

TSDSTLGGVT

SVLANQKIKD

104 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

EF023-3 (SEQ ID NO:87)

GGGAACOGGA

CAAAAAATTG

GATACGACCT

GAAGATGAAT

GTTTATACAT

AACCACTTTG

GCTTTATTTT

AATACTCTTG

ACCCCTTATT

ATCAAAGAAC

TTTAAATTGA

ACCTATTGGG

CCAACGACAG

GGTGAATTTA

ACATACTTTT

AACAATATTC

TTGCAAGATC

CATGGAACAG

GCGAAAGCAA

TTAGATT'

CAATTGGAAG

CGTGTTGATC

TATCGTGATC

ACGTTCAACA

GATCAAGAGG

ACGGTTTTAG

GATCTGTATTI

AACTCGGCTG

CAATTAGTTC

CG.ACTTTAGT

TAGAGTTGGG

TAACATAG

TTTATGCAT-G

TTGATGTCAT

GGGTTAAGGC

TGAAATCATT

A.AGGGGATAA

AAGAATGGGA

ATCCTGAAAA

CGGTGAATT

TTCCTGGTTA

TAAAAATGAA

GTAAAGCGTT

AATCAACGGC

ATTTCACAAA

AAGAATTCTG

TAACAGATGA

AAAATCTAGA

GTGATCAAAC

CATTAACAGT

GTGATACGTA

CTCGTTTAAA

CACCAATCTA

GGCATTCATT

ATAGTAACAA

TCAAGCGGCT

CATGAATCAA

GGTCGCTGCC

AGAAGATGCC

GCAACAAGTT

TAAAAATGCT

GCTAGATGAT

ACTTTCGTT

ATATGCTACT

TAATGCCTCT

AGTTAAATTA

GTTTGACTCG

TGTTGATAAT

CAGCGTTCGT

GGCACAAGCT

TACAGATCAA

ACTAGCTGCT

GGAAAAAGG

TACAGACAGC

TGGATTAGAA

GAGAGACTAT

TATGCGCATC

TGATCAATTA

TGACTTITGCT

CAATCGAAGC

TGGACCCACG

AGCAGCGGAA

ATTTCGACAA

GTTTATGAAG

GAAGAACCAG

AAATGGTCGA

GC ITCCCCTA

AAGGA-AATTG

AAAACGTTAG

CCTGTTTTGT

6GLTGCAGAAC

TCTGATGACT

ACAGAAGCGA

AATGA.ATTGG

CCAGCC=rC

GATGGAAAAG

TTI'GATAAAG

GTAATTCCC

AAGAAAAATA

AAAAAAGAAA

GCCAAAAAAG

GTGAATGTTA

GA'ITTAGAAT

TTTACTTCGG

ATTCAAGAAA

CAAGCACAAG

ATTI'CTGTAT

TACAGTTTAA

TGGAACCACA

GACTCTATGT

CGATTTCTGA

ATCATGATCC

AATCAGGATC

CTTTAGAACG

AAATAACTTT

TITCCACAAAA

AT'ITATTTA

GGACCTACGA

AAG'ITTCAGT

ATCGTAGTGAA

TTTCAATTCC

AAAATCCGGC

AAAGTT'TGT

CGGGACAAAC

ACTACTTAAC

TTGGGCTGGA

C--GCTGAGTT

CTCAAGT1'CC

TATCTGGTTG

ATAGTACCT

CTAGAACAAC

ATATGGT

TAGCTAATCA

AATGGGCTTA

CACAGCGGGG

TTTAGGTAAA

AGATGAAACC

AGTAACACCA

GATTCATCAA

CGCAGATG-TG

AGAACGGCCC

TGAAAAATAT

TAATGGTCCT

AAAAAATGAT

AATTAAGAC

TAAGCTAAGT

TCA.ATTCGTC

TTTAGCGAAC

AAAACA.AGTC

GATTGCGCCA

CTACGATACA

TAAA.ATCAAA

TTTCCAATTT

TTTTACTATT

GGGAACCGAT

GGGCGGCGTA

ACATG-CGGCT

GAATCAGGAA

AAAAATCAAG

T13TTAAC 0* e.

0* EF023-4 (SEQ ID NO:88) GTGN SADSNKAAEQ KIAISSEAAI STMEPHTAGD

TTSTLVMNQV

DFVYAWQQVA

PYLKSLLSFP

YWDAEKVKLT

YFLKNNSVRD

GTDFTKLAAK

LEENLDGLEV

FKSDTYDQLI

YEGLIYVLGKE

SPKSGSIHQA

VLFPQNEKYI

EAKVSVIKSP

GKENPALANN

KNNYLTYDTA

NVTQVPFTIR

QETRTTHAAD

DELELGVAAE

LFFDVIKNAK

KEQGDKYATO

TTAVNLFDSN

NIRKALAQAF

KAKEFWEKGK

VDRDQTRDYJ

QEARLNDFAQ

EPAISEDETV

EIALEGADVN

A.EHLIYNGPF

ELDVVNKLSG

DKESFVKEVL

KEIGLDKIKL

LELSGWGTDY

AQDILVNQET

YTFKIREDAI(

TLGVKALDDK

KLKEWDNASS

EFIPGYVDNP

QDQSTATDQV

EFLTDDTDSA

RDPLTVMRIF

VLAPIYNRSI

WSNDDPVTAN

TLEITLERPT

EDDWTYEKNDT

AFLS IPQFVT

IPPGQTIAPD

KKAAEFFQFQ

TSDSTLGGVT

SVLANQKIKD

LYWHSFGPTY SLKWAYVN EF024-1 (SEQ ID NO:89)

TAATGGCCGT

AACAAGGATC

GTCGCCTTCT

GGTGGTAAAT

T'CAGCTA

AAAAAAATAA

AANGNTANCA

ACAGAATAA

TTCGTCTACT

ATAAAAAAGG

TA ITGTI'GTC

GGAAAGTGGA

ATGACTCAGA

CCTI'TGACNT

AGATTACAGG

AATAAAGAGG

AGAAGTGAGC

AGGTTGTGGA

AGAAACACGT

GGATAGTGTT

TACTAGCAGN

GGAAATTGC

ATGAAGCTAC

ATGAAAAAAG

ACAGATATGA

GCAACTTACA

AGTGGGACAT

AACTCTTTCA

GAAAAACAAA

TCAAATGGCG

TACTACCI'I'

AAAAGATATT

CTTnI'TTTGA

ACACTTATGA

TTATGGAAAA

GAACACTTAT

TTGGCAATGG

TATTGCC'ITA

GACTGCCGAT

TGACGGTAAA

TGAAAAAAAT

AGTNGANTNC

AAAACAAAAA

105 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

E-F024-2 (SEQ ID M KKVLPFIALV GLLLLSGCGT DMKILTADG GKWKVEETRA TYTFFDDGKF SANDSEDSVS GTYTYDEKNK KITFIDXTSXN SFIMEKVXXX XXKITGEIGE KQRTLIKQKT E EF024-3 (SEQ ID NO:91) ATT GACTGCCGAT GGTGGTAAAT GGAAAGTGGA AGAAACACGT TTTrCAGCTA ATGACTCAGA GGATAGTCTT AAAAAAATAA CCI=TGACNT TACTAGCAGN AANGNTANCA AGAPI'ACAGG GGAAATTGCC

ACAGAA

GCA.ACTTACA

AGTGGGACAT

AACTCTTTCA

GAAAAACAAA

CTTI'ITTGA

ACACtTATrGA

TTATGGAAAA

GAACACTTAT

TGACGGTAAA

TGAAAAAAAT

AGTNGANTNC

AAAACAAAAA

EF024-4 (SEQ ID NO:92)

LTADG

GKWKVEETRA TYTFFDDGKF SANDS EDSVS XXKITGEIdE KQRTLIKQKT E GTYTYDEKNK KITFDXTSXN SFIMEKVXXX EF025-1 (SEQ ID NO:93)

TCAATGAAAC

GCGCCAGATC

AAGCAAGITA

AATGACGAAC

GTTACAGGGG

GAAAAAAAGA

TA'ITATAAAA

GAACCTGAAA

TATGATACAC

AGCAACACGC

AAACAAATC

ACTTCTGCGC

CCATCGAAAG

CTTCAAGTGA

CATGAACAGG

ATTCACCAGG

ATATTAAAGG

AAGAGCCAAC

CCGTCACCAA

TGATTGCTAA

CCACACAAAC

AAAAAATCTT

ATGAAGGTGT

CGCAAAGGAT

CCTA

4 FrCCG

GTACAGACGA

GTGACGAAAA

GTGAAGGATT

AAACGTATAT

TAACAGAAGA

AAAAAATCAC

ATACAATAAA

AATGTTGGTT

GAAACAAACA

TCAAACGACT

TCAATTGACT

GACATTTGGA

TTGGTCCAAT

ATTTACTGC

TCTGAATGTT

TGTCAACAAA

TACGTI'AGTC

TCGTGTTACA

AATGCCTTTA

CGGTTACGCA

GCAGAAAATA

AGAAACAGCC

CAAACCAACA

TTTATCGGAT

ACAAGTGGTC

TCTGCGGTG

TTTGATTCTC

ACAACCCCAC

CAACCGCCTT

GGAAATTACG

GAGTTTACAG

CCC CTGTCGG ACCGTT GTTA

GGTAAT'TT

GCAGCAGAGA

GAAGATTTAG

AAACATGTCA

TGYCGCCTT

ATTCTTTTAT

TATTTATTTI

CGCAAGAGAC

AAAAACA.AGC

ATGAATACAC

CAGCAAAATA

TGGGATTAAT

GCATTGTAGA

AGTTTGCTAG

ATTATCCTGA

ATCCrTATTAC ATACG4GTACG

TGGACACT=

GCAATGGCCT

GCTATGATGA

TTATCGTCAA

TCATTI'TGTA

GTTGTTGT

AAAGCAAGTG

CCCGACTAAA

GTACCAAGTG

TACACCGGAA

GACGGGTAAC

GATTATTACG

TGATCCTTAT

ATTTCAAGCA

TTATGTAGAA

CGG'ITCATCA

GCTAAAAGAG

AATCGCTCGA

ATACTTTTCA

TCGAAATATT

G

EF025-2 (SEQ ID NO:94) MKH IKGMLVF

DELIANQLTF

YKNECVFTGG

NTRTDDTLVT

SKETYICYkE

HQDTINKPTI

IGLJFILVGCA

DSHEYTYEVV

NYGIVEI ITE VVNG ITYVTEK

DLGNGLIARL

LLFIL

PDQEPTKQTT

TGATQTTFGT

PETQRILNVE

QMRDENRVTG

QVITEEQKIK

SGPQETKQVI(

TPPAKYTPEE

FI'EFASD)PYY

NFYTVRGSST

HVSYDEYFSD

QVIVTNQTTS

KKKKMFWSNQ

DTRYSGVNK(R

SAREGLMPLA

EQEK ITE:TAC

AVEKQAPTKN

PPLGLMTGNY

LSDYPEFQAS

A.EMDTWLKEP

GLFIVNRNI I EF025-3 (SEQ ID 106 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

AAC GAAACAAACA ACAAGTGGTC CGCAAGAGAC AAAGCAAGTG

AAGCAAGTTA

AATGACGAAC

G ETACAGGGG

GAAAAAAAGA

TATTATAAAA

GAACCTGAAA

TATGATACAC

AGCAACACGC

AAACAAATGC

ACTTCTGCGC

CCATCGAAAG

CTTCAAGTGA

GATGAACAGG

ATTCACCAGG

CCGTCACCAA

TGATTGCTAA

CCACACAALAC

AAAAAATGTT

ATGAACCTCT

CGCAAAGGAT

GCTAT1'CGGG

GTACAGACGA

GTGACGAAAA

GTGAAGGATT

AAACGTATAT

TAACAGAACA

AAAAAATCAC

ATACAATAAA

TCAAACGACT

TCAMA1'GACT

GACATTTGGA

F1'GGTCCAAT

ATTACTGGC

TCTCAATGTT

TGTCAACAAA

TACGT1'AGTC

TCGTGTTACA

AATGCCTTTA

CGG'ITACGCA

CCAGAAAATA

AGAAACAGCC

CAAACCAACA

TCTGCGGTGG

TTTGATTCTC

ACAACCCCAC

CAACCGCCTT

GGAAATTACG

GAGTTTACAG

CGCCTGTCGG

ACCGTTGTTA

GCTAATTTT

GC6AGCAGAGA

GAAGATTTAG

AAACATGTCA

TGCGCCTT

ATTCTTTTAT

AAAAACALAC

ATGAATACAC

CAGCAAAATA

TGGGATTA.AT

GCATTTAGA

AGTTTCGCTAC

ATTATCCTGA

ATGGTATTAC

ATACCGTACG

TGGACACTI'G

GCAATGGCCT

GCTATdATGA

ITATCGTCAA

TCAT=TG

GCCCACTAAA

GTACGAAGTG

TACACCGGAA

GACGCGTAAC

GATTATTACG

TGATCCTTAT

AITTCAAGCA

TTATGTAGAA

CGGTTCATCA

GCTAAAAGAG

AATCGCTCGA

ATACTTTTCA

TCGAAATATT

EF025-4 (SEQ ID NO:96) TKQTIT SGPQETKQVK QVTVTNQ'FrS AXTEKQAPTI(N DEL IANQLTF DSHEYTYEVV TGATQTI'FGT TPPAKYTPEE YKNEGVFTGG NYGIVEIITE PETQRILNVE FTEFASDPYY NTRTDDTLVT VVNGITYVEK QMRDENRVTG NFYTVRGSST SKETYIGYAE DLGNCLIARL QVITEEQKIK HVSYDEYFSD HQDTINKPTI LLFIL KKKKMFWS NQ

DTRYSGVNKR

SAREGLMPLA

EQEK ITETAC

PPLGLMTGNY

LSDYPEFQAS

AEMDTWLKEP

GLFIVNRNI I EF026-1 (SEQ ID NO:97) TGAGTGTATrG

TTTTTTGGAG

GCTGCTCTTG

AGCAAAGAAA

CCTGAAGAGC

AATGTCACTI'

TCCAACCATG

GCTGAATATG

CACGGGACTG

AAAGTTAATG

AAAGATGGTG

ATTACTCATT

GGATITTCAT

TGrI'GCTTAG

CAGCTAATTC

TCGAAATGC

TTGATAAAGA

TCTATCGTAA

AAATCACGGT

TCAAAGGTGA

GCAATATTAC

CCACTG'?rAC

TCCCTTTGAA

GAAAATGTCT

TGCTTGTTCA

AAGTACAGAA

GTTAAGTGAT

GGTAACAGTT

ATTACCACTTl

TCCTAAGCTA

TATTGAGGTG

TTTTGATAAA

TGGTGAAGTC

TCAGTrATGA

AALAGTACTCA

TCTGATAAAA

GTAGTCTCTG

AAAGGAAATI'

GCTGGTACTr

TATTCCCAAG

ATCGTTTC~l'

AAAGCAAATG

CAAGAATACA

AC CGTAGC CA

TAAAGGAAGA

CCACTGTTTT

AAACAGATAG

GTGCTTCAAT

GGATTCTC-GC

TCCATGATAA

ATGATAATAA

CTGA.AAATTT

GCTTTACTTT

AAGATrCTGC

ATAA'FIAA

AATAAATA-AA

GACGGCAACT

TAGTTCTAGT

TAGTGCCAAG

AGC'IACTGAC

GGGGAAAGAT

AAAAGTAACT

CAACATCGTT

AAATGGTACC

TGACI'AGAA

*f EF026-2 (SEQ ID NO:98) MXMSK VLTIVLTATA ALVLLSACSS DKKTDSSSSS KETANSSTEV VSGASISAKP EELEMALSDK GNWIVATON VTFDYEVTVA GTFHDKGKDS NDVYRXLALY SQDDNKKVTA EYEITVPKLI VSSENPNIVH GTVKGDIEVK ANGFTLNGTK VNGNITFDKQ EYKDSADLEK DGATVTGEVT VANN EF026-3 (SEQ ID NO:99) AACAGATAG TAGTTCTAGT AGCAA.AGAA.A CAGCTAA'TC AAGTACAGAA GTAGTCTCTG GTGCTrCAAT TAGTGCCAAG CCTGAAGAGC TCGAAATCCC GTTAAGTrAT AAAGGAAAI' GGATTGTCGC AGCTACTGAC AATGTCACTT TTGATAAAGA GGTAACAGTT GCTGGTACT1' TCCATGATAA GGGGAAAGAT 107 TABLE 1. Nucleofide and Anfino Acid Seqeuences of Efaecalis Genes.

TCCAACCATG3

GCTGAATATG

CACGGGACTG

A.AAGTTAATG

AAAGATGGTG

TCTATCGTAA

AAATCACGGT

TCAAAGGTGA

GCAATATTAC

CCACTGTTAC

ATTAGCACT

TCCTAAGCTA

TA'FIGAGGTG

TITTGATAAA

TGGTGALACTC

TATTCCCAAG

ATCCTTTC IT

AAAGCAAATIG

CAAGAATACA

ACCCTAGCCA

ATGAT.AATAA

CTGAAAATTT

GCTTTACTTI

AAGATTCTGC

ATAAT

AAAAGTAACT

CAACATCGTT

AAATGGTACC

TGACTTAGAA

EF026-4 (SEQ ID NO:100)

TDSSSSS

KETANSSTEV

NOVYRKLALY

VNGNITFDKQ

VSGASISAKP EELEMArJSDK SQDDNI<KVTA EYEITVPKLI EYKDSADLEK OGATVTG-EVT GNWIVAATDN VTFDKEVTVA GTFHDKGKDS VSSENFNIVH GTKGDIEVX ANGFTLNGTI( VANN EF027-1 (SEQ ID NO:101)

TTTGGTATGA

GCATGTGGAA

GTCGCATCTG

TCCGATATGA

GAGCTAGCTA

AAGTTACGAG

GCGTTTAGAA

TTTAAAAAT±G

GCAATCGATG

GTCTTGGTTG

GCCTATGGGA

GATGGCAAT

GTAAAATTGA

TTTGATAATG

GAGTCAAAAC

CGCCGTGAAA

GAAACTTTTG

GCTAAT

TGCCATATAA

AACAGAAAAA

GTGGCGGTI'C

GTGGTGAACT

TTGGTCAAGT

TGGCGAAAC

AAGCAAAATG

ACGTGGTCGA

GGCGTGCGGT

ACCAGACACT

GGACACCTT

C'FrCTCCAGA

CCGAAACTTG

ATGAAATTGA

GCGACTTAGA

AAGCGCATr

TTACCGGCAA

CAAAAGAAAT

CAAAAATCCA

TATTAAAGAA

GTGGTTAATC

GAAAACGACC

CTCGACATTA

AGTTGAAGGC

AGAGCCACAA

GACAAACGGG

TCCAGCATAC

GCGGGAAGGA

AGAACTAACA

TATGCCTAAA

TAATTTI

GAAA'ITGAAG

TGTrCAAGTA

TTACACTGT

TGTACCTA.AA

CGAACATGTI'

TTTAGGAGAT

GATACAGGTG

GCCCAAGCTA

GGACTTIGTTG

TCAAACGA.AC

GACAGCGCTC

TTGTATCGAC

GTTACTGAAG

GATCCAG=A

GGTTCAAGTA

CAAGCCACGA

TTGGAAAATC

AATGAAGCCT

GGCAATGGGC

AAGAATGATC

GTGAAAGAAA

TACAGATA

GCCATGGTG

CGAAAAGC 1T

GGCTCGACAG

AAGATCCG

ACTGGAACAA

CACTGGGCTT

CAGCTACACA

ArATACAGA AAGATAkAAA

ACGGGTTAGT

AAGCAGGGGA

GCAGTAATCA

TGGA-AGCATT

CAATTCCTTA

TTGCCAAAGA

CGTIGTLAAT

ATTATTGGGA

TTGrGCACAGG

CTI'ATCCACT

G ITTAAAG

TTIACAAGC

C'FTrAAATGG

CAAAGAAAAT

TT

GGTrTTAGCA

GAA.AATTAAC

TGTCTATAGT

CGGAGATCCT

CTATACATTC

TTTTGTAGTT

AATGGATATT

TGGTGTCAAA

TTTAGCCCAA

AAAAGGTACT

TTCAGC FrGG

TAAAGAACAC

AGCCAATCTT

TCAGTATAAA

CCCCAATCAT

GATTGACAAA

NTTTGTACCA

GGTGATTTAT

EF027-2 (SEQ ID NO:102) MKQKKWLI GLVALGLVLA ACGSGGSKTT SNEPATQKIN VASGGELSTL DSAHYTDVYS SDMIGQVVEG LYRQDKNGDP E LAMAKAEPQ VSEDGLVYTF KLRE.AK;4TNG DPVKAGDFVV AFRNVVDPAY GSSSSNQMDI FKNGRAVREC QATMEEFGVK AIDDQTLELT LENPI PYLAQ VLVGTPFMPK NEAFAKEKGT AYGTSADNFV GNGPFVISGW DGNSETWXrLK KNDHYWDKEH VKLNEIDVQV VKEIGTCANL FDNGDLDYIV LADTYALQYK ESKQA}{FVPK AMVGYLSPNH RREITGNEHV RKAFLQAIDK ETFAKEILGD GSTALNGFVP ANFAKIQIQV KISAXKM1VIY CHIILKKPKL TGTI EF027-3 (SEQ ID NO:103) AACGACC TCAAACGAAC CAGCTACACA GAAAATTAAC GTCGCATCTG GTGGTGAACT CTCGACA ETA GACAGCCCTC TCCGATATGA TTGGTCAAGT AGTrGAAGGC TTGTATCGAC GAGCTAGCTA TGCCGAAAGC AGAGCCACAA GTTAGTGAAG AAGTTACGAG AkAGCAAAATG GACAAACGGG GATCCAGTTA GCGTTTAGAA ACGTGGTCGA TCCAGCATAC GGTTCAAGTA

AITATACAGA

AAGATAAAAA

ACGGGTTAGT

AAGCAGGGGA

GCAGTAATCA

TGTCTATACT

CGGAGATCCT

CTATACAITC

'TTTGTAGTT

AATGGATAT

108 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

?FTAAAAATG

GCAATCGATG

GTCTTGGTTG

GCCTATGGGA

GATGGCAATT

GTAAAATTGA

TTGATA.ATG

GAGTCAAAAC

CGCCGTGAA.A

GAAAC=TTG

GCTAATTr'rG

TGCCATATAA

GGCGTGCGGT

ACCAGACACT

GGACACCTI'

CTTCTGCAGA

CCGAAACTTG

ATGAAATTGA

GCGACTTAGA

AAGCGCATTT

TTACCGGCAA

CAAAAGAAAT

CAAAAATCCA

TATTAAAGAA

GCGGGAAGGA

AGAACTAACA

TATGCCTAA

TAATTTTGTT

GAAATTGAAG

TGTTCAAGTA

TTACACTGTTI

TGTACCTAAA

CGAACATGTT

TTTAGGAGAT

GATACAGGTG

CCCCAAGCTA

CAAGCCACGA

TTGGAAAATC

AATGAAGCCT

GGCAATG-GGC

AAGAATGATC

GTGAAAGAA.A

TTAGCAGATA

GCCATGGTGG

CGAAAAGCTT

GGCTCGACAG

AAGA ETTCCG

TGGAAGAAT

CAATTCCTTA

TTGCCAAAGA

CGTTTGTAAT

ATTATTGGGA

TTGCCACAGG

CTTATGCACT

GTTATI'AAG

~T=ACAAGC

CTTTAAATGG

CAAAGAAAAT

TGGTG-TCAAA

TTTIAGCCCAA

AAAAGGTACT

TTCAGGTTGG

TAAAGAACAC

AGCCAATCTT

TCAGTATAAA

CCCCAATCAT

GATTGACAAA

NTTTGTACCA

GGTGA ITIAT EF027-4 (SEQ ID NO:104) TT SNEPATQKIN VASGGELSTL DSAHYTDVYS SDMIGQVVEG LYRQDKNGDP EIJAMAKAEPQ VSEDGLVYTF AFRNVVDPAY GSSSSNQMDI FKNGRAVREG QATNEEFGVK VLVGTPFMPK NEAFAXEKGT AYGTSADNFV GNGPFVISGW VKLNEIDVQV VKEIGTGANL FDNGDLDYTV LADTYALQYK RREITGNEHV RKAFLQAIDK ETFAXE-ILGD GSTALNGFVP

CHIILKKPKL

KLREAKWTNG

Al DDQTLELT

EDGNSETWKLY

ESKQAHFVPI(

ANFA.KIQIQV

DPVKAGDFVV

LENP IPYLAQ

KNDHYWEDKEH

AM'VGYLSPNH

KISAKKMVIY

a.

C.

EF028-1 (SEQ ID NO:1OS) S S *4

TAACAGAAGC

AAGACTTG'rr

AGAGCTIGC

TCTGAACAGA

GAAAAAGCAT

ACAACGGGCT

TTrGATACCT ACCGA ITCAG

ATTGCACTCG"

GGGAAATCAA

GGCGCACATA

CAAATCGACG

CGGAAAGATC

AAAAAGTCGT

CTACCTAAAA

GCTCTTCAAC

ATTGATTGGG

GAAGCGGCGT

CAACTGCAGA

AATACAACAA

ATAGTCAATG

TAGGGGTTAC

AAAGCGGCGA

CAGTAAAAAA

ATCGCTATT

ATTTGGTCGG

CTTCCGCAGC

ATAATGACAA

CGGGTCTTGT

ATGTICACG

GACA.ACACAA

GTGATTTAGT

TAAATGAGAA

TGATTGACCG

GGTTAGATAA

CCGGGCATAG

ITGAAA.AGGC

TCATTCAACA

CTTAACACTT

TATGGGTAGA

CTTATTAACA

AAAACAAACA

TGTTATTTT

CAAAGCCAAT

ACAGCAAGCC

GACACATG

GTCCAAAACA

AGCAACATCT

CAAAAATATG

AGTCGATGTG

CAAAGAATTT

CCAAGACGAC

AACGGAAGAA

AAATGAAAAA

CAATGATATT

CATCGATTTT

GGGGGCTTGT

TGTTTACTTG

TATGAAr.GAG

TTCACAACAT

GAGGTTGCTG

ATGATTGGAG

CACTCAGACA

ACTTATCCAG

GCTGCCGGAG

TTATTTATCA

GAAACAAGGA

TAGCGGGTTG

A.AGCGAAGGC

ATGGCATGGG

AGCGTrGTTCC

AAGATGAAGA

TGAAAACCTA

GAAACAGTGC TCGAACGTGC GAAAAAAGTG GAAATAACAC ATGCAACCCC TGCTGCATAT

GAAATCAACT

AATGAAGAAA

TACAAATTTA

AACTGAATCT

GAATCCGTAT

CCAA.ACAGCT

AGAGAATGTC

TAATAATGCT

GCAGAAATCG

TTAC'ITGGCG

TCCCAAGCGG

AAAATTTAG

GTCCCTTCAT

GG ITTC'TTT

GTTGGCGCGA

GCCAAAAAAG

CTIAG

CCGATGACTA TTrTGATGAT GCGGCTCCGA ATTATITGCCC GTTATGGTCA TGTCACAGAC GCTTGTTTGC ACCAGGCGGG TAGCTOATAT GACAGAACG TALATGGTTGA AGGTAGTCAA TGAGCGAAAT GCAAGACTTC ATGGTGAACA TTG.GTGGTTA EF028-2 (SEQ ID NO:106) MKKR ALLGVTLLTF TTLAGCTNLS EQKSGEKQTE VAEAKATESE KASVKNVIFM DTYLVGQQAT YPEDEEENVr DSASAATAMA KSTGLVATSE ITH-ATPAAYG AHNVSRKNMA KDRDLVKEFS QAGYGHVTDK KSLNENQDDK LQRLDKNEKG FFLMVEGSQI DWAGHSNDIV IGDGMGNPYT TGYRYFKANH AGVKTYNNAI ALDNDKSXTE EIADDYFDD)Q IDGQMXVDVL ILGLFAPGGL PKMIDRTEEV GAMSEMQDFE AAFEKAIDFA

SDKRVPQTAF

TVLERAKXVG

LGGGSEILFAR

PSLADMTEAA

KXDGEHWLQ

109 TABLE 1. Nucleotide and Amino Acid Seqeuences: of E. faecalis Genes.

LQI IQQGACL EF028-3 (SEQ ID NO:107) ACAGA AAAGCGGCGA AAAACAAACA GAGGTTGCTG AAGCGAAGGC AACTGAATCT

GAAAAACCAT

ACA.ACGGGCT

TTTGATACCT

ACCGATTCAG

ATTGCACTCG

GGGAAATCAA

GGCGCACATA

CAA.ATCGACG

CGGAAAGATC

AAAAAGTCGT

CTACCTAAAA

GCTCTTCAAC

ATTGATTGGG

GAAGCGGCGT

CAACTGCAGA

CAGTAAAAA

ATCGCTA=F

ATTTGGTCGG

CTITCCGCAGC

ATAATCACAA

CGGGTCTITGT

ATG TCACG

GACAACACAA

GTGATTTAGT

TAA.ATGAGAA

TGAT~rGACCG

GGTTAGATAA

CCGGGCATAG

ITGAAAAGC

TCATTCAACA

TGTTATT=r

CAAAGCCAAT

ACAGCAAGCC

GACAGCGATG

GTCCAAAACA

AGCAACATCT

CAAAAATATG

AGTCGATGTO

CAAAGAAT'T

CCAAGACGAC

AACOGAAGAA

AAATGAAAAA

CAATGATATT

CATCGATTT

GGGGGCTTGT

ATGATTGGAG

CACTCAGACA

ACTTATCCAG

GCTCCCGGAG

GAAACAGTGC

GAAATAACAC

GCAGAAATCG

TTACTTGGCG

TiCCCAAGCGG

AAAATTTTAG

GTCCCTTCAT

GGTTTCTTTT

G ITGGCGCGA

GCCAAAAAAG

CTr

ATGGCATGG

AGCGTGTTCC

AAGATGAAGA

TGAAAACCTA

TCGAACGTC

ATGCA-ACCCC

CCGATGACTA

GCGGCTCCGA

GTTATGGTCA

GCTTGTTrGC

TAGCTGATAT

TAATGG~rGA

TGAGCGAAAT

ATGGTGAACA

GAATCCGTAT

CCAAACAGCT

AGAGAATGTC

TAATAATGCT

GAAAAAAGTG

TGCTGCATAT

TTTTGATGAT

ATI'ATT1TGCC

TGTCACAGAC

ACCAGGCGGG

GACAGAAGCG

AGGTAGTCAA

GCAAGACTTC

TTG-GTGGTTA

EF028-4 (SEQ ID NO:108) QKSGEXQTE VAEAKATESE KASVKNVIFM IGDGMGNPYT TGYRYFKANH SDKRVPQTAF DTYLVGQQAT YPEDEEENVT DSASAATANA AGVKTYNNAI ALDNDKSKTE TVLERAKKVG KSTGLVATSE ITHATPAAYG AHNVSRKNMA EIADDYFDDQ IDGQHKVDVL LGGGSELFAR KDRDLVKEFS QAGYGHVTDK KSLNENQDDI( ILGLFAPGGL PKMI DRTEEV PSLADMTEAA LQRLDKNEKG FFLMVEGSQI DWAGHSNDIV GAMSEMQDFE AAFEKAIDFA KXDGEHWWLQ LQI IQQGACL EF029-1 (SEQ ID NO1:109)

C

TGAAGGAGGG

GCCACTII'TT

GAAGTTACTG

GTI'AGGCACG

CGG'rrTTTAT

GGATTGGAAG

GAATATGATA

GATGGAACAT

AAAGAGCCTA

GAGTCACTCG

AGAAAATGAA

TATTATCAGG

AAAATGTTAC

TGGATGGCGA

TAATTGACAC

CTAGCAAACG

AGGGCGATAA

TACTACAAAA

CAACTAAGTA

GA.ATCTGGAG

AAAGTTAATC

ATGCGCAAGT

TCAAACTAT

TACCACACTA

ACCCGAGACT

CACAAAAGAG

AACAGATCGT

AACGCTTGTA

TTTGGCAGAA

CATACCAGGT

GCTAAAAACT

CTTGAACAAA

TCAAACGATC

TTAAAAATTG-

GTGAAACCGA

CTTTTGTCTA

TACGGACGAG

AGTGAAGGAT

CTAGAGCAAG

TATCTGACAC

GGCTGCTGCT

AAGCACAGGA

AACGTATACC

ACCGAAAAGA

AAACAAA.AGT

CTGCTTCAGA

C= IGrGCTA

TAGCTCGTGT

CCCAAGAACA

AMCGGGGO'TT

TACAGCAGTA

TAGTGTAAAA

AGCTGATTTT

ACAAAAAGTT

TCAGCCGTTC

AA'FrACGTTT

CATATTCGTA

TGCCTATGTA

GGCTAAAALAT

TAGTAAATAA

EF029-2 (SEQ ID NO:110) MKKLIG KKWLLLTAVA TFLLSGCASL EQKAQDSVKE VTENVTQTIS NDQRIPADFV RHVDGDTTVL KIDGKEQKVR FLLIDTPETIV KPKTKVQPFG LEASKRTKEL LSTASEITFE YDKGDKTDRY GRALGYIFVD GTLLQKTLVS EGLARVAYVK EPTTKYLAEL EQAQEQAKNE SLO IWSIPGY VTQRGFSK EF029-3 (SEQ ID NO:111) AAATGTTAC TCAAACTATT TCAAACGATC AACGTATACC AGCTGATTTT GTTAGGCACG TGGATGGCGA TACCACACTA TAAAAATTG ACGGAAAAGA ACAAAAAGTT 110 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

CGGTT'FIAT

GGATTGGAAG

CAATATCATA

GATGGAACAT

AAAGAGCCTA

GAGTCACTCG

TAATTGACAC

CTAGCAAACG

AGCCCGATAA

TACTACAAA-A

CAACTAA GTA

GAATCTGGAG

ACCCGAGACT GTGAAACCCA AAACAAA-AGT

CACAAAAGAG

AACAGATCGT

AACGCTTGTA

TTTGGCAGAA

CATACCAGCT

CTTTTGTCTA

TACGGACGAG

AGTGAACCAT

CTAGAGCAAG

TATGTGACAC

CTGCTTCAGA

CGTTGGGCTA

TAGCTCGTGT

CCCAAGA-ACA

AACGGGGGITT

TCAGCCGTTC

AATTACG=~

CATATTCGTA

TGCCTATGTA

GGCTAAAAAT

TAGTAAA

EFC29-4.(SEQ ID NO:112) NVTQTIS NDQRIPADFV RHVDGDTTVL KIDGKEQKVR FLLjIDTPETV K-PKTKVQPFG LEASKRTKEL, LSTASEITFE YDKGDKTDRY GRALGYIFVD GTLLQKTLVS EGLARVAYVK EPTTKYLAEL EQAQEQAXNE SLGIWSIPGY VTQRGFSI( EF030-1 (SEQ ID NO:113)

TGATTGACAC

GTTACTACGG

ACTGAGAAAA

TCAATGGATT

GAAGGGTTAT

CCAGAAArT

TCCA.ATGGTG

CCCAAAAACC

ATTACAA.AAG

TTGGAGGI'A

TTTTTCCCAC

GAAATGATTG

TGGGATITG

CATTTTGAAG

GATGTGGCTG

GCAATCGAAC

TCCtATTITT

TTAGTCGATA

TTTGTATATA

ACAGACCCCA

GTAGCCATTG

ATAGGGGGAA

TCTTGGTAGG

CCAGTGTCTC

CTAT ITTTAC

ATCAATTGGA

CTGAGGATGG

ATGCTGTAAC

AAGCCAATTA

AGGAAAAAGC

C ITTAGAAAA

AAAACGAAGC

TATCTAATGG

TGCGTAATCC

TTCTTAAAGA

TCTTAACAGG

GGTCAAAAGT

CAAATGAA

ATATTTTAC

ACCCAGAAAC

AAAAAGCCAA

AACTTCTTTC

TAGTATGAAA

TTGTGGCACA

GAAAA.AAGTT

ACAAGATGAA

TGAAA.AAGAT

CAAACGATAT

AGCCAATCAT

CTTTTTCTTG

ACCAGAGGAA

GCCTGTACCA

ATTCGTGAAA

TCCGTTTTA

CTACTATTAC

AACCAATACC

AGATTTGCT

CTATTCCTTA

TGTCCGCAAA

AGATGGCTCA

GAATGAAGAT

AGAGTATTTA

AAGAGATGGT

AAGTTAAAA.A

ACAGCAGANA

TTAAATTTAA

GCCAGTATTA

CAACTAATAC

ACCATTAAAC

TI'CGT'TTTG

TTAGAAGGAA

TTGGGTGTCA

TATTTTACGT

GAAAAAGGAC

ATGAAAAATT

GATAAAGAAA

G ITATAAT

AAACAAAATC

CGT'IAAACC

GCT'IAGCTT

AAAGAAATTT

TITCGTCAAG

GATAAACCAA

GATAGTGACC

TGATGGGGAT TATGTTATTT CAAAA.ATACA CGAGAAAGCA TGGAGAACTC GGAAATCGGT ACGCACAGT'C CAATGTCTTT CTGCTGCTGC TAAAGAGATG TAAGAGAAGA TGGCAAGTGG C'FrGCGTAA ATTAGCGAAT CGATI'CTGAA CGGAACAGCT AAGCGCTTGA TGATTATACT CGFI'ATTGGC ATTTTCTCCA AAGCCTATGG CACTTCTAGT GGGATCAGTC AGCGATGTCG AAGTAAAATC AGAAACGATT TGTACGAATIC AGGTGAATTA GAGACAACCC AGACTATGAA

AAAAAAGAAA

ATCCTTTGGA

ATGGGTACAT

AACCAGGCGC

AAGCAGAGCT

GA

CGAAAA.ACCA

TAAAAAAAGT

TCCAGAAAAA

TCTTGTCAAA

AAACGGAGAT

C EFC30-2 (SEQ ID NO:114) MKX LKMMGIMLFV STVLVGCG ET AXTXIDEKAT EKTSVSKKVL NIMENSEIGS MDSIFTQDEA SINAQSNVFE CLYQLDEXDQ LIPAAAKEMP EISEDGKRYT IKLREDGKWS NGDAVTANDF VFAWRKLANP KNQANYFFLL ECTILNGTAI TKEEKAPEEL GVXALDDYTL EVTLEKPVPY FTSLLAFSPF FPQNEAFVKE KGQAYGTSSE MIVSNGPFLM KNWDQSAMSW DFVRNPYYYO KEKVKSETIH FEVLKETNTV YNLYESGELD VAVLTGDFAI( QNRDNPDYEA IERSKVYSLR LNQKRNEKPS IFANENVRKA LAYALDXXSL VDNILADGSI( EIYGYIPEKF VYNPETNEDF RQE.AGALVKT DAXKAKEYLD KAKAELNGDV AIELLSRDGD SDR EF030-3 (SEQ ID NO:115)

GAGAAAGCA

ACTGAGAAAA CCAGTGTCTC GAAAAAAGTT 'TAAATTTAA TGCAGAACTC GGAAATCGGT TCAATGGATT CTATTTTTAC ACAAGATGAA GCCAGTATTA ACGCACACTC CAATGTCTT'r GAAGGGT1'AT ATCAATTGGA TGAAA.AAGAT CAACTAATAC CT-GCTGCT'GC TAAAGAGATG TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

CCAGAAATIT

TCCAATGGTG

CCCAAAAACC

ATTACAAAAG

TTGGACGTTA

TTTTCCCAC

GAAATCATTC

TGGGATTTTG

CATTTTGAAG

GATGTCGCTG

GCAATCGAAC

TCCATT1'TG

TTAGTCGATA

TTTGTATATA

ACAGACGCCA

GTAGCCATTG

CTGAGGATGG

ATGCTGTAAC

AAGCCAATTA

AGGAAAAAC

CTTTAGAAA

AAAACGAAGC

TATCTAATGG

TGCGTAATCC

TTCTTAAAGA

TCTTAACAGG

GGTCAAAAGT

CAAATGAGAA

ATATI'ITAGC

ACCCAGAA-AC

AAAAAGCCAA

AACTTCTTTC

CAAACCATAT

AGCCAATGAT

CTTTI'CTTG

ACCAGAGGAA

GCCTGTACCA

AITCGTGAAA

TCCGT'TTITA

CTACTATTAC

AACCAATACC

AGATTITTGCT

CTATTCC'ITA

TGTCCGCAAA

AGATGOGCTCA

GAAMAACAT

AGAGTATTTA

AAGAGATGGT

ACCATTAAAC

TTCGT1rTIG

TTAGAAGGAA

TTCGCTO;TCA

TAT'TTTACGT

GAAAAAGGAC

ATGAAAAATT

GATAAAGAA.A

GTTTATAATT

AAACAAAATC

CGTTTAA.ACC

TA.AGAGAAGA

CTTGGCGTAA

CGATTCTGAA

AAGCGCTTGA

CGTTAITGGC

AAGCCTATGG

GGGATCAGTC

AAGTAAAATC

TGTACGAATC

GAGACAACCC

A-AAAAACAAA

TGGCAAGTG

ATTAGCGAAT

CGGAACAGCT

TGATTATACT

ATTTTCTCCA

CACTTCTAGT

AGCGATGTCG

AGAAACGATT

AGGTGAATTA

AGACTATGAA

CGAAAAACCA

TAAAAAAAGT

TCCAGAAAAA

TCTTGTCAAA

AAACGGAGAT

GCTTTAGCTT ATGCTTTGA AAGAAAT'F ATGGGTACAT TTCGTCAAG AAGCAGCGC GATAAACCALA AAGCAGAGCT EF030-4 (SEQ ID NO:116) EKAT EKTSVSKKVL NLMENSEIGS MDSIFTQDE-A SINAQSNVFE GLYQLDEXDQ NGDAVTANDF VFAWRKLANP KNQANYFFLL EVTLEKPVPY FTSLLAFSPF FPQNE.AFVKE DFVRN~PYYYD KE-KVKSETIH FEVLKETNTV IERSKVYSLR LNQKRNEKPS IFANENVRKA VYNPETNE-;DF RQEAGALVKT DAKKAKEYLD LIPAAAKENP EISEDGKRYT EGTILNGTAI TKEEKAPEEL KGQAYGTSSE MIVSNGPFLM YNLYESGELD VAVLTGDFAK LAYALDKKSL VDNILADGSK KAXAELNGDV AIELLSRDG

IKLREDGKWS

GVKALDDYTJ

KNWDQSAMSW

QNRDNPDYEA

EIYGYIPEKF

EF031-1 (SEQ ID NO:117)

TGAGAAATTA

AAACGCGTAA

AGCCAAGCAA

TTCGGATTAA

GAAGCGAAAG

AATCCCAATG

AAAGATGGGT

CCGGGAGCAA

GGG.ATTGTTT

TCAGCTGATT

ATGTTrATACG

GCTTTTGAAG

GACTAGCN'A

GTTCATATTA

GTTATTTTAG

'ITTTAGGGAC

CTACGAAAAG

GTGAAGATAT

TTACC7I'AGA

CGGGAATTGA

TATFI'GAAAA

AAGAGGTTi'

ACAACAAAGA

TGAAAGGTAA

TTGCTAGTGA

CGATGAAAGA

ATATGTTCCA

TTCAAGGCGC

AAAAATAA.AA

ATTAGTCGCT

CGAGAGCAAA

TGTCAAAAAA

AGTAGGCAAT

TGTCATTGAA

AATTACAGAA

TGAAAGTGGT

AAAATTAGGC

AATTTCTGTT

ACATGCTGGT

ATTAAAACCA

ATCTGGTGAA

ACAGAAAACG

ACCAT=GG

GCAACGTTAT

GGTGGAAGTA

GACATTATCG

AGCGCAGACC

TTAGCACAAG

AAAGAACTAC

GCTGGCGTAC

AAAGAAATI'A

CCAGACG TC

CAAGATATTA

AACGTTGTTA

ATTGAACCAG

TGA

AGGAAGA=T

TAATGACTGC

ATGC'IIAGT

CTCCATTI'CA

GCTTACGAA

CAAATGCAGC

CTAAT'ITAAC

CAATCGCTGT

AAAACTGGGA

CCACCACGGC

cAAAAGATAA AAACGTAT1'C cTGTcGGMC

AAAAATGAAA

TTGTGGAA-AC

CG'TTCAACT

AAAAGAGAAT

ATTAAAAAAT

ACAAGGTGGA

TCAGTTAACG

AAACAGTATC

TGACTTATGG

AGGTCCTITA

CGGGAAGGCC

AAAATCGTCA

TCATTTTGCG

S S EFOO31-2 (SEQ ID NO:118) MYK RVILGTLVAA TLLMTACGNS E-ATTKSESKG GSRALVVSTF GLSEDIVKKD IIAPFEXENE AXVTLEVGNS ADRPTKLKNN PNAGIDVIEL AQANAAQGGK DGLFEKITEK EVPNLSQLTP GAKEVFESGA GVPIAVNSIG IVYNKMGLK EIKNWDDLWS ADLKGKISVP DVATTAGPLM LYVASEHAGQ DITKDNGKAA FEAMKELI(PN VVKTYSKSSD LANMFQSGEI EAAVVADFAV DIIQGAQKT EF031-3 (SEQ ID NO:119) 112 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

AA CTACGAAAAG CGAGAGCAAA GGTGGAAGTA ATGCTFAGT CGTTTCAACT

TTCGGATTAA

GAAGCGAAAG

AATCCCAATG

AAACATGGGT

CCGGGAGCAA

GGGATTGTTT

TCAGCTGATT

ATG ITATACG

GCTTTTGAAG

GACTTAGCNA

GTTGATATTA

GTGAAGATAT

TTACCTTAGA

CGGGAATTGA

TATTTGAA-AA

AAGACCFTT

ACAACAAAGA

TGAA.AGGTAA

TTGCTAGTGA

CGATGAAAGA

ATATGTTCCA

TTCAACGCOC

TGTCAkAAAAA

AGTAGGCAAT

TGTCATI'GAA

AATTACAGAA

TCAAAGTGGT

AAAATTAGGC

AATTTCTGTI'

ACATGCTGGT

ATTAAAACCA

ATCTGGTGAA

ACAGAAAA

GACATTATCG

AGCGCAGACC

TTAGCACAAG

AAAGA.AGTAC

GCTGCCGTAC

AAAGAAATTA

CCAGACGTTG

CAACATATA

AACG=rTTA

ATTGAAGCAG

CTCCAT?1'GA

GCTTI'ACGAA

CAAATGCAGC

CTAA=~AAG

CAATCGCTGT

AAAACTGGGA

CCACGACGGC

CAAAAGATAA

AAACGTATTC

CTGTGGTTGC

AAPLAGAGAAT

ATTAAAAAAT

ACAAGGTGGA

TCAGTTAACG

AAACAGTATC

TGACTTATGG

AGGTCCTTA

CGGGAAGGCC

AAAATCGTCA

TGATTTTGCG

EF031-4 (SEQ ID NO:120) TTKSESXC GSNALATVSTF GLSEDIVKKD I IAPFEKENE AKVTLEVCNS ADRFTXLKNN PNAGI DVIEL DCLFEKITEK EVPNLSQLTP GAKEVFESGA GVPIAVNSIG IVYNKEKLGK ADLKGKISVP DVATTAGPLM LYVASEHAGQ DITKDNCKAA FEAMKELKPN LANMFQSGEI EAAVVADFAV DIIQGAQK

AQANAAQGGK

EIKNWDDLWS

VVKTYSKSSD

EF032-1 (SEQ ID NO:121)

TGAATAAATT

GTTTGTGGTA

AAGTCAACCA

TCAGGGGAAT

CAACTAGATG

AACCATGACT

CTCACATTCG

CAAGAATATA

TCTGATGT T

GAACTAAGTA

AATACTAATG

GATACTTAG

TCAATTATCG

ATTTAGGAGG

TTTCACTACT

GTCAATCTAG

ATTCAGTTGG

ATAAATCGAG

TATACGGAGT

AAACTCCCGA

TGAAAAATCC

CTAAAAGTAG

CTGAAGCG;A

ACATTIACTAA

CTAAGCATCA

GCCTCATCCC

AATTATGATG

TACTGCTTGT

CAGCACAGTT

AAAAGATATT

CATAGTTCT

GGGAAACAAG

CAAAGATTT

ACTATCNAGT

TAGCCAAGAT

GTCTGATGTA

GCTAGCAGAT

ATITAATGAT

AACCATGGAC

AAAAAATI'AA TTAGT=TAGG NCGGGAAATA ATGATAATAA AAACAACCGA ATTCAAAAGA GATCCTGGAG ATTACTATGC ATTACCGTCA AATCAGGCGG AAAAA.AGTAT CTCTTAAAAA GTTGTTAGAT TTTTAAATGA ACTGAAACTA GCAAACANAA AATAAACAAT CTGATGTATC GCTACTAATA CTTTACCGAG CAGCCAACCT TAGAACAACA ATGTATCCTI' ATAAAGGAAG GCAAAAAGAT GGTAA

ATTGGTTTIGT

AGATACTCAA

CTTTGTTGCG

TGTATTAACT

AGAAAATAGT

GGGAGATACT

AAA.AGATTTT

AACACTAAAC

TGAAAAAAAA

CGAAGATAAA

AACCGTCTTA

CAAAATGCAT

C- EF032-2 (SEQ ID NO:122) MK KLISLGLVCV CGISLLTACX GNNDNKDTE( STSQSSSTVK QPNSKDFVAS GEYSVGKDID PGDYYAVLTQ LDDKSSIVLJI TVKSGGENSN HDLYGVGNKY, KVSLKKGDTL TFETADKDFV VRFLNEKDFQ EYMKNPVSST ETSKXKTVNS DVSKS SSQDN KQSDVSEKKE VSTEAKSDVA TNTLPSEDKN TNIJITKLADE PTLEQQTVLD TLAKEQFNDM YPYKGSKMHS IIGVIPTMDA KRW EF032-3 (SEQ ID N'O:123) TA ATGATAATAA AGATACTGAA AAGTCAACCA GTCAATCTAG CAGCACAGTT AALACAACCGA ATTCAAAAGA CTTT'CTTGCG TCAGGGGAAT ATTCAG IrGG AAAAGATATT GATCCTGGAG ATTACTATGC TGTATTAACT 1.13 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecais Genes.

CAACTAGATG

AACCATGACT

CTCACATTCG

CAAGAATATA

TCTGATGTT

GAACTAAGTA

AATACTAATG

GATACTTTAG

TCAATTATCG

ATAAATCGAG

TATACGGAGT

A.AACTGCCGA

TGAAAAATCC

CTAAAAGTAC

CTGAAGCGAA

ACATTACTAA

CTAAGCATCA

GCGTCATCCC

CATAGTTCTT

GGGAAACAAG

CAAAGA=TT

AGTATCNAGT

TAGCCAAGAT

GTCTGATGTA

GCTAGCAGAT

ATTTAATGAT

AACCATGGAC

ATTACCGTCA

AAAAAAGTAT

GTTGTAGAT

ACTGAAACTA

AATAAACAAT

GCTACTAATA

GAGCCAACCT

ATGTATCCTF

GCAAAAAGAT

ALATCAGGCGG

CTCTTAAAAA

TLTAAATGA

CCAAACANAA

CTGATGTATC

C:TTTACCGAG

TAGAACAACA

ATAAAGGAAG

GG

AGAAAATAGT

GGGAGATACT

AAAAGATTTT

AACAGTAAAC

TCAAAAAAAA

CGAAGATAAA

AACCGTCTTA

CAAAATCCAT

EF032-4 (SEQ ID NO:124) NDNKDTEK STSQSSSTVK QPNSKDFVAS GEYSVGKDID PGDYYAVLTQ LDDKSSIVLI TFETADKDFV VRFLNEKDFQ EYfrflNPVSST 'JSTEAKSDVA TNTLPSEDKN TNDITKLADE IIGVIPTMDA KRW TVKSGGENSN H-DLYGVGNXK KVSLKKCDTL ETSKXXTVNS DVSKSSSQDN KQSDVSEKKE PTLEQQTVLD TLAKNIQFNDM YPYKGSKMHS EF033-1 (SEQ ID NO:125)

TGACTGCT'T

CAAAGGAGGT

AAAATGAAAA

GCAGGATGTG

TTACCAACCA

ATr'TCCCTAG

ATCGCAGTTG

GATATGATG

AATGACATCA

ACAGTCGTTA

ATCGCTGATA

GAAATCGACG

TTTTCTATTG

TCXITTCAGA

AATTTACTTT

GTAAACAGGA

AAGACCGTAG

TGCCATCAAC

ATACTCAAAG

CTGTCGATGC

ATTTAGCTAG

ATATCCCCAC

GCITATCTGA

AGTAG

GAGAAAAAAG

AA.ATTTTCCC

AACAATGATG

AAAGAAAGCA

CGGCAAAGAA

AACAGAAGTG

TAGTACAATG

CGAAAAATTG

CTCAGAAAGT

TAGTACAAGC

ACATGAAAAA

TGGTTTTTTT

CAAAATA-AAA

ACTTIAGG'

ACTACCTCIT

AI'ACTTTAC

ATTGAAGACT

ATGACTGATT

ATTGCCTTGA

GT'TGCAAGC

ATCAAAGCAA

GGACAAAAGT

GTATTGI=

TAGACGAATCG

TAG.TAGCAAC

CTGAAAAAAC

CCAAAGAAGC

TAGGTAAAAC

TAAAAAAATT

A-ACCACAAAT

AAGTGGAAGA

TCAAAGAAGA

TAATCAAAAC

GACGTTGAGA

CGAGGATGAA

ACTTGGCTTA

AGAAGTAACG

AACCAAAAT

CGACCAATTA

ACCACAAATG

TGTTTATGTG

TGCTGGAAT

CGTCCAATTC

AATGGATCAA

EF033-2 (SEQ ID NO:126) MKKFTLTMMT LGLVATLGLA GCGKQEKXAT TSSEKTEVTL AVDTQSSTMMh TDLKKLPQMD VVNIPTSTSI KAIKEDVQFI

PTKDRSGKEI

MMfAVDAEKLI

ADSLSEH-EKG

TLPKEATKII

ALKPQIVYVN

QKLIKTMDQE

SLVPSTTEVI EDLGKTDQLI DINLASSESV WKQVEDAGIT

IDE

EF033-3 (SEQ ID NO:127) CTCTT CTGAAAAAC AGAAGTAACG TTACCAACCA AAGACCGTAG ATTTCCCTAG TGCCATCAAC ATCGCAG ETG ATACTCAAAG GATATGATGG CTGTCGATGC A.ATGACATCA ATTTAGCTAG ACAGTCGITA ATATCCCCAC ATCCCTGATA GCTIATCTGA GAAATCGACG AGTAG

CGGCAAAGAA

AACAGAAGTG

TAGTACAATG

CGAAAAATTG

CTCAGAAAGT

TAGTACAAC

ACATGAAAAA

ATTACITTAC

A~GAAGACT

ATGACTGATD

ATTrGCCTTGA

GTTTGGAAGC

ATCAAAGCAA

GGACAA;LAGT

CCAAAGAAGC

TAGGTAAAAC

TAAAAAAATI'

AACCACAAAT

AAGTGGAAGA

TCAAAGAAGA

TAATCAAAAC

AACCAA.AATr

CGACCAATA

ACCACAAATG

TGTTI'ATGTG

TGCTGGAATT

CGTCCAATTC

AATCGATCA-A

114 TABLE 1. Nucleotide arnd Amino Acid Seqeuences of E. faecalis Genes.

EF033-4 (SEQ ID NO:128) SSEKTEVTL PTKDRSGKEI TLPKEATKII SLVPSTTEVI EDLGKTDQLI AVDTQSSM TDLKKLPQMD MAVDAEKLI ALKPQIVYVN DINLASSE-Sv WKQVEDAGIT VVNIPTSTSI KAIKEDVQFI ADSLSEHEKG QKLIKTMDQE IDE EF034-1 (SEQ ID NO:129)

TAGGAGGGAG

TTGGTAGGTT

CCTT~TAATAC

TTCAACA.AGG

GACACAAAAA

AkATAAAGAGA

AAAAAAGTTG

TAATCATGAA

GTAGTAATAA

TCACCACGAT

ATAAAACCAT

AAACAAGTAG

GCTATGAAAT

ATGA.AGGTAA

AAAAATCGGG

CAAAAAAGAA

TATTGAAAAA

GACGCTTGAA

AACAGAAAAA

TATAGGTCA-A

ACGTATATCT

TATfl'TAG1r1

AACGGCAATC

GAAGAAGACC

AAAGAATAT

AAGGTATATA

'ITGGACAAAA

GATGCAGA-AG

GTATTA'ITTT

'-TTTGAATGC

TAACGAAAGG

TAGTTAATCC

A-AAATATTAA

AAACGAAAAA

GTAATGTGTA

TTCATGT

CAGTTCGI-r

TTCAATTTITL

CAATAATGAA

AATACAAGAA

AATAGAGTTT

TGGTGAT=~

(ft ft GGTGGTAAAT AG EF034-2 (SEQ ID NO:130) MKKIGY FSCIIFFMFL VGCSNNKKEN GNLLNASSFP LILTTIIEKE EDLTKGSIFF NKDKTMTLEK EYLVNPNNED TKKTSRTEKK VYKNIKIQEN KESYEIIGQL DKKTKKIEFK KVDEGKRISD AEGNVYGDFG GK EF034-3 (SEQ ID NO:131) AGAA AACGGCAATC TTTTGAATGC CAGTTCGTTT CCTTTAATAC TCACCACGAT TATI'GAAAAA GAAGAAGACC TTCAACA.AGG ATAAAAC CAT GACGCTI'GAA AAAGAATATT GACACAAAAA AAACAAGTAG AACAGAAAAA AAGGTATATA AATAAAGAGA GCTATGAAAT TATAGGTCAA TTGGACAAAA AAAAAAGTITG ATGAAGGTAA ACGTATATCT GATGCAGAAG GGTGGTAAAT AG

TAACGAAAGG

TAGTTAATCC

AAAATATTAA

AAACGAAAAA

GTAATGTGTA

ITCAATFI'TT

CAATAATGAA

AATACAAGAA

AATAGAGTTT~

TGGTGATITT

ft EF034-4 (SEQ ID NO:132) KEN GNLLNASSFP LILTTIIEKE EDLTKGSIFF NKDKTMTLEK EYLVNPNNED TKKTSRTEKK VYKNIKIQEN KESYEIIGQL DKKTKKIEFK KVDECKRISD AEGNVYGDFG GK EF035-1 (SEQ ID NO1:133)

TAAACGAGAG

TTATTCACAA

ACAATTGATC

GAAAATTCCA

AAAATCGATA

GTGAGTITAT GAAAACAAAA ATCGGAAAAA CAGTTATCIT GTCAGCAT GTTTCCTTIP ACTGAGTGGT TGTACCTCGG CTGGCGAAGA GATGGAAAAA GACAGAAAGA AAAAGTCGAT AAAACGGTCG ATA.AGCAGAA ACATAAAAAT TGGAAAGTTA CGACGAAAAA GTTIGACCGTT CTTTAGATAG TCAAGAAGAC CTACTGAGTA A EF035-2 (SEQ ID 1.1:134) MK'TKI GKTVILSAFL FTSFLLLSGC TSAGEEM~EKT IDRQKEI(VDK TVDKQKHXNE NSMESYDEXV DRSLDSQEDK IOTTE 115 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecczlis Genes.

EF035-3 (SEQ ID NO:135)

GATGGAAAAA

ACAATTGATC GACAGAAAGA AAAAGTCGAT AAA.ACGTCG ATAAGCAGAA ACATAAAAAT GAAAATTCCA TCGAAAGTTA CGACGAAAAA CTTGACCGTT CTTTACATAG TCAAGAAGAC AAAATCGATA CTACTGAG EF035-4 (SEQ ID NO:136) MEKT IDRQKEKVDK TVDKQKHKNE NSMESYDEKV DRSLDSQEDK IDTTE EF036-1 (SEQ ID NO:137)

S

S

S

5555

TAATTTTCAA

AATGAATCGA

ACAGGATGTG

TTACAACCAT

ATTAATGTCC

GACATTGGTA

ATTGATCATA

AA.AGATATCT

GAACTTGGCG

CGTGCGACTT

GATTCCAGCG

GCATTT1TCAT

GATGAAAATG

AAAAATCCAA

GAACGTGTGA

TGGCAAGGAA

GTCCTACATA

TGAAAAAAAG

CAAAATGGAT

TAGTAGAGAC

AAGGTGGCGG

ATTCTGA~T

AAGTTGCTGT

CAATGGAAAA

GGAAAGACCA

TTGAAAAGTG

GCATGGTTCG

ATGTTACTGA

TAATGAACAA

GTGATTTAAC

TTGGTCAATT

ATGTCATTAA

TAATCGTAAA

ATTGCTATTA

TGATCGTGGT

AGCGAGTGAG

AAGCGGAACA

ATTIGCAGAA

CGTTGGGATT

TTI'AAAGAAA

AAAAATTGTT

GGTCTTGGGA

TTCCATTOTT

TGAAGTAGCT

TAAATGGATT

CAAAGAGTTI'

AGGGTATA T

ATAA

ATAGAATGGA TTGAAA'ITAA TTTATTG7 TGGCAAGTAT GAATCCATCA CAGCGGTAGG GAATATCAAA GCCAAAATCC GGTCTGAGTC AACTCCAATC GAGAAAAAGG GCATCAAAGC ACACCAkATCG

ATCTTITTAG

ATTTTGAATA

GATAAAACAG

TCTGATACAC

ACGTTAAGTA

A1"rGTCTT

TI'AGACTTTA

CCTGTICGA

TTAACAAAAA

GTGAAGTAAC

GAGCGGCCGG

CCATTCGTGC

CAGGAGCGAT

TTGATGGTGT

ATGAACACAT

TGTTCTCAGA

AAATGGAAAT

rI'GGAGGAAT

ACTTACTI'TG

CTCATCAGCT

GGGAAGATTT

TGGCGCGGTA

GGAAGACTTA

TGTCGGTGTC

AAACTGGAAA

TAGTGGTACG

GCAAGAACAA

TACTTATACC

TCAGCCAACA

GTACACTCGT

TGATATCCAA

TGAACGGGAT

EF-36-2 (SEQ ID NO:138) ~'*MKKRLLLF IGLASILTLT GCAXWIDRGE SITAVGSSAL QPEJVETASEE YQSQNPGRFI NVQGGGSGTG LSQVQSGAVD DHKVAVVGIT P IVNKNVGVK DI SMENLXKI FLGEVTNWXE ATFEKWVLCD KTAIRAQEQD SSGMVRSIVS DTPGAISYTA ENVMNNKWII WSYEHMYTRK NPSDLTKEFL DFMLSDDIQE

QGNVIK

IGNSDLFAEE

LGGKDQKIVI

FSYVTDEVAT

RVIGQLGYIP

KKGIKAEDLI

LNRAAGSGTR

LS IDGVQPTD

VSKMEIERDW

EF036-3 (SEQ ID NO:139) GAT TGATCGTGGT GAATCCATCA CAGCGGTAGG CTCATCAGCT

TTACA-ACCAT

ATTAATGTCC

GACAITGGTA

ATTGATCATA

AAAGATATCT

GAACTTGGCG

CGTGCGACTT

GATTCCAGCG

TACTAGAGAC

AAGGTGGCGG

ATITCTGATTT

AAGTTGCTGT

CAATGGAAAA

GGAAAGACCA

TTGAAAAGTG

GCATGGTTCG

AGCGAGTGAG

AAGCGGAACA

ATTTGCAGAA

CGTTGGGATT

TTTAAAGAAA

AAAAATTGTT

GGTCTTGGGA

TTCCATTGTT

GAATATCAAA GCCAAAATCC GGGAAGATTT GGTCTGAGTC AAGTCCAATC TGGCGCGGTA GAGAAAAAGG GCATCAAAGC GGAAGACTTA ACACCAATCG TTAACAAAAA TGTCGGTGTC ATCT ITrTAG GTGAAGTAAC AAACTGGAA-A ATI'TGAATA GAGCGGCCGG TAGTGGTACG GATAAAACAG CCATTCGTGC GCAAGAACAA TCTGATACAC CAGGAGCGAT TAGTTATACC 116 TABLE 1. Nucleotide and Amnino Acid Seqeuences of E. faccalis Genes.

GCAT=rCAT

GATGAAAATG

AAAAATCCAA

GAACGTGTGA

TGGCAAGGAA

ATCTTACTGA TGAAGTAGCT ACGTTAAGTA TAATGAACA-A TAAATCGATT AITTGGTCTr GTGATTI'AAC CAAAGAGTTT TTAGACTTTA TTGGTCAATT AGGGTATATT CCTGTTTCGA ATGTCATTAA A TTGATMGTGT TCACCCAACA ATGAACACAT GTACACTCGT TGTTGTCAGA TGATATCCAA AAATGGAA.AT TGAACGGGAT EF036-4 (SEQ ID NO:140) IDRGE SITAVGSSAL QPLVETASEE YQSQNPGRFI NVQGGGSGTG DHKVAVVGIT PIVNKNVGVK DISMENLXKI ATFEKWVLGD KTAIRAQEQD SSGMVRSIVS ENVMNNKWI I WSYEHMYTRK NPSDLTKEFL

QGNVIK

LSQVQSGAVD

FLGEVTNWKE

DTPCAI SYTA

D)FMLSDIDIQE

IGNSOLFAEE

LGGKD)QKIVI

FSYVTDEVAT

RVIGQLCYIP

KKGIKAEDLI

LNRA.AGSGTR

LS IDGVQPTD

VSKIMEIEROW

EF037-1 (SEQ ID NO:141)

TGAGTGTATG

TTTTTTGGAG

GCTGCTCTTG

ACCAAAGAAA

CCTGAAGAGC,

AATCTCACTT

TCCA-ACGATG

GCTGAATATG

CACGGGACTG

AAAGI'AATG

AAAGATGOTG

ATTACTCATI'

GGATTT"TCAT

TGTTCCTI'AG

CAGCTAATTC

TCGAAATGGC

'1WGATAAAGA

TCTATCGTAA

AAATCACGGT

TCAAAGGTGA

GCAATATTAC

CCACTGTTAC

TCCCTTTGAA

GAAAATGTCT

TGCTTGTTCA

AAGTACAGAA

GTTAAGTGAT

GGTAACAGTT

ATTACCACTT

TCCTAAGCTA

TATTGAGGTG

TTTTGATAAA

TGGTGAAGTC

TCAGTTATGA

AAAGTACTCA

TCTGATAAAA

GTAGTCTCTG

AAAGGAAATI

GCTGGTACI'

TATTCCCAAG

ATCGITTCTT

AAAGCAAATG.

CAAGAATACA

ACCGTAGCCA

TAAAGGA.AGA

CCACTGTTTT

AAACAGATAG

GTGCTTCAAT

GGATTGTCGC

TCCATGATAA

ATGATAATA.A

CTGAAAATT

GCTTWTACTTT

AAGATTCTGC

ATAA

AATAAATAAA

GACGGCAACT

TAGTTCTAGT

TAGTGCCAAG

AGCTACTGAC

GGGGAAAGAT

AAAAGTAACT

CAACATCGTT

AAATGGTACC

TGACTTAGAA

EF037-2 (SEQ ID NO:142) MKMSK VLTTVLTATA ALVLLSACSS DKKTDSSSSS KETANSSTEV VSGASISAKP EELEMALSDK GNWIVAATDN VTFDKEVIVA GTFHDKGKDS NDVYRKLALY SQDDNKKVTA EYEITVPKL I VSSENFNIVH GTVKGDIEVK ANGFTLNGTK VNGNITFDKQ EYKDSADLEK DGATVTGEVT VANN EF037-3 (SEQ ID NO:143) AACAGATAG TAGTTCTAGT

AGCAAAGAAA

CCTGAAGAGC

AATGTCACTT

TCCAACGATG

GCTGAATATG

CACGGGACTG

AAAGTI'AATG

AAAGATGGTG

CAGCTAATTC

TCGAAATGGC

TTGATAAAGA

TCTATCGTAA

AAATCACGGT

TCAAAGGTGA

GCAATATTAC

CCACTGTTAC

A.AGTACAGAA

GTTAAGTGAT

GGTAACAGTT

ATTAGCACTT

TCCTAAGCTA

TATI'GAGGTG

TTrTGATAAA

TGGTGAAGTC

GTAGTCTCTG GTGCTTCAAT AAAGGAAATT GGATTGTCGC GCTGGTACT'T TCCATGATAA TATTCCCAAG ATGATAATAA ATCGI'TCTT CTGAAAAUTTT AAAGCAAATG GCTTTACTTT CAAGAATACA AAGA~rCTGC ACCGTAGCCA A

TAGTGCCAAG

AGCTACTGAC

GGGGAAAGAT

AAAAGTAACT

CAACATCGTT

AAATGGTACC

TGACTIAGAA

EF037-4 (SEQ ID NO:144)

TDSSSSS

KETANSSTEV VSCASISAXP EELEI4ALSDK NDVYRKLALY SQDDNKKVTA EYEIT'JPKLI GNWIVAATDN VTFDKEVTVA GTFHDKGKDS VSSE-NFNIVH GTVKGDIEVK ANGFTLNGTI( 117 TABLE 1. Nucleotide and Amino Acid Sequences of Efaecalis Genes.

VNGNI TFDKQ EYKDSADLEK DGATVTGEVT VANN EF038-1 (SEQ ID NC:145)

TAATGGCCAT

AACAAGGATC

GTCGGCTrGT

GGTIYGTAAAT

GAAAC=~TT

AATAATAAAA

GAATATAAAG

TNGAATAAGA

TTCCTCTACT AATAAAGAGG ATAAAAAAGG AGAAGTGAGC TATTGTTGTC AGGTTGTGGA GAACTAGA AAATAAAAGT CGAGGTATAA TTCAAAA.ATT- AACTCACTTT GGATATAAAA ACGGTAAATT AAAAGGTGAA OGTGTCTTTG A

ATGA.AGCTAC

ATGAAAAAAG

ACAGATATCA

CCAACTACTA

AGTGATAGTG

AATAAAGAAC

ATi'GGGCG

TCAAATGGCG

TACTACCTTT

AAAAGATATT

CTTACACTTT

GA.ACGTACTC

A.ATI'AATAAT

AGAAGGACTC

TTGGCA.ATG

TATTGCCTTA

GACTGCCGAT

'FFITCATGAT

TTACGATdAA GGkAAATGTT

TGATAAAAAA

EF038-2 (SEQ ID NO:146) M KLLKWRWQWN KDHKXGEVSM KKVLPFIALV GLLLLSGCGT DWKl(ILTACG GKWELENXSP TTTYTFFDDE TFSRYNSKIS DSGTYSYDEN NKKLTLDIKN KEQLIMENVE YKDGKLKGEI GGEKDSDKKX NKRCL EF038-3 (SEQ ID NO:14'7)

S

at..

S

TTGTGGA ACAGATATGA AAAAGATAT GACTGCCGAT GGTGGTAAAT GGGAACTAGA AA.ATAAAAGT CCAACTACTA GAAAC rITI I CGAGGTATA.A T'rCAAAAATT AGTGATAGTG3 AATAATAAAA AACTCACTTT GGATATAAAA AATAAAGAAC GAATATAAAG ACGGTAAA'T AAAAGGTGAA ATTGGAGGCG TNGAATAAGA CGTGTCTTTG A

CTTACACTTT

GA.ACGrACTC

AATTAATA.AT

AGAAGGACTC

TTTTGATGAT

TTACGATGAA

GGAAAATGTT

TGATAAAAAA

EF038-4 (SEQ ID NO:148) CGT DMKKILTADG GKWELENKSP TTTYTFFDDE TFSRYNSKIS DSGTYSYDEN NKKLTLOIKN KEQLIM.ENVE YKDGKLKGEI GGEKDSDKXX NKRCL EF039-1. (SEQ ID NO:149)

TAAATATATC

GCGCTTACCT

TCTGCAACAG

GCAGCTAAAG

CAAGGCACAA

A=GGTTAG

GGTGAAACCC

TTAATTTCGG

AAATTTACCT

GAACATATCC

ACAGAAACTG

TGGAATGGCA

TITATrAGTT

ACGGGTAAAA

GCTCACGTT

GATAAACGTT

AAAAAGAAAA

TATTAACCTT

ATAAATCAAG

AGCAATCAAA

AAGTTTACGA

CAAAATATCA

GTrGGCGATGA

ATACACAAAA

ATAAGCGAAT

C'rrATTCTGA

GCAAGATTGT

CGAAAGIT'IT

TAGC.GAAATT

CACGTCGAGA

CAATTGGTGA

TACGTATAC

AAGGGGATTA

TAGTACGTTG

TGCAGCTAGC

AGGACAAGAA

CAAAAATNAT

TGGTGAAACA

AGGCACA ETC

CTATCAAGCG

GGGTAAAGAT

CGAGAAATTA

TACCAATGAA

AGATGAAGAC

TGATAATAAA

TTITGGFI'AC

CAATAAATAT

ACGAATGGCT

CCAACCATCA AAAAGAAAA.A AGTITTAGT TTGA'ITGCAG GCTGTGCTGG CGGAGCCAAC

TCAAGCACTG

TTAACAGAAA

AATAATTTAA

GGTTITI'ATG

TTTGTGACAG

GTGGTCGATT

AAAGACGGGA

ACCT ITACGA

CCTGGGGATG

GGTAACGATG

CAGTCTCTAG

TTTTATCCAG

CAGCAGAAAA

AA EvTTITCGA

ACCATGCCGA

TAACGGAAGT

AAGATGTAGA

ACGrGCCGTAA

ACATT=AGG

TTACTGAAGC

TTCAGCAGAA

TACTGATTGG

TGcTAATTI'T

CAAAGAAACA

AAAGCGTATC

GACGAAAGAT

AGTCTTTGTA

AGATAGA-A

GCCCACATTA

AAATAAAATG

TGATTTAGAA

CAAAA'TCCGT

AGAATTAAAT

AATTAAAGTC

ACAAGTAAAT ATGAATICTT TTCCAAGTAA ?TGATAATAA GGAGCTGCAT TAGA.ATTA-AC AP.AGACA.ACA ATCGCAAAGA 118 TABLE 1. Nucleotide and Amino Acid Seqcuences of E.faecalis Genes.

TTTGTAGAAC ATGAACCATA TGAAGGAGAC TTTACGCCAG ACTTCACGTT CTAA EF039-2 (SEQ ID NO:150) MXKKKVFSA LTLLTFSTLL IACCAGGANS ATDKSSAASS STAVSSSAEA AXKEQSKGQEL TEILSSTDWQ CTKWYDKNXN NLTAENANFI GLAKYDCETG ETRGDEGTFF VTDDGEXRIL ISDTQNYQAV VDLTEVTKDK FTYKRMGKOK HIPYSDEKLT FTNGRKDLET ETGKIVTNEP GDIDILGATLW NGTKVLDEDG ISLA.KFDWKT SKYEFFDLET GKTRGDFGYF QVIDNNKIRA HVSIGDNXYG KRFTYTRMGK DUNGKEIKVF VEHEPYEGDF TPDFTF

FYEFFDKETG

DGKDVEVFVE

NDVTEANKMF

AALELTELND

EF039-3 (SEQ ID NO:151) TGCAACAG ATAAATCAAG TGCAGCTAGC TCAAGCACTG CAGTCTC TAG TTCAGCAGA.A i* S

CCAGCTAAAG

CAAGGCACAA

ATTGGTTTAG

GGTG.AAACCC

TTAATTCGG

AAATTTACCT

GAACATATCC

ACAGAAACTG

TGGAkATGGCA

TTTATTAGTT

ACGGGTAAAA

GCTCACGFI

GATAAACGTT

TTTGTAGAAC

AGCAATCAAA

AAGTTTACr-A

CAAAATATGA

GTGGCGATGA

ATACACAAA-A

ATAAGCGAAT

CTTATTCTGA

CCAAGATTGT

CGAAAG=TT

TAGCGAALATT

CACGTGGAGA

CAATI'GGTGA

TTACGTATAC

ATGAACCATA

AGGACAAGAA

CAAAAATNAT

TGGTGOAAACA

AGGCACATTC

CTATCAAGCG

GGGTAAAGAT

CCAGAAATTA

TACCAATGAA

AGATGAAGAC

TGATAATAAA

T=IGGTTAC

CAATAAATAT

ACGAATGGGT

TGAAGGAGAC

TTA.ACAGAAA

AATAATTTAA

GGTTTTTATG AATTT=TCGA TTTGTGACAG ACGATGGCGA GTGGTCGATT TAACGGAAGT AALAGACGGGA AAGATGTAGA ACCTTACGA ACGGCCGTA CCTGGGGATG ACAITIAGG GGTAACGATG TrACTGAACC ACAAGTAAAT ATGAAITCTT TTCCAAGTA.A TTGATAATAA GGAGCTGCAT TAGAATrAAC AAAGACAACA ATGGCAAAGA TTTACCCCAG ACTTCACCTr

TTTATCCAG

CAGCAGAAAA

TACTGATTGG

TGCTAATTTT

CAAAGAAACA

AAAGCGTATC

GACGAAAGAT

AGTCT'ITGTA

AGATTTAGAA

GGCCACATTA

AAATAAAATG

TGATTTAGAA

CAAAATCCGT

AGAA'ITAAAT

AATTAAAGTC

CTAA

EF039-4 (SEQ ID NO:152) ATDKSSAASS STAVSSSAE-A AKEQSKGQEL TEILSSTDWQ GTKVYDKNXN ETRGDEGTFF VTDDGEKRIL ISDTQNYQAV HIPYSDEKLT FTNGRKDLET ETGKIVTNEP ISLAKFDNKT SKYEFFDLET GKTRGDFGYF KRFTYTRM4GK DNNGKEIKVF VEHEPYEGDF

NLTAENANFI

VOLTEVTKDK

GOD ILGATLW

QVIDNNKIRA

TPDFTF

GLAKYDGETG

FTYKRGKDK

NGTKVLDEDG

HVS IGDNI(YG

FYEFFDKETG

DGKDVEVFVE

NDVTEANKMF

AALELTELND

EF040-1 (SEQ ID NO:153) S S

TAGAITAGAA

ATGAACAAA.A

TGTCAAGGAG

AGTAGTGCAA

GAGGAAGTGA

CAACGAGATA

GTCGACACAG

GCAAAAGCGT

CCCTATCTTT

GCGACCCCAG

GAAAAAGGAC

TTCCAAAATG

CAACTTTACC

GACAATTCTG

CCACTGGAGA

AAATTTTAAT

GAGAAACACC

AAACGAAAG

AAGAACCGAT

CGAAGA.AGGT

AAGGCAACAC

TGATTGATCG

ATGCGGGAGC

ATTTAA.ATAA

ATATTGACAA

ACTT'TGGCTA

AAGATATTGT

GTCGAACAGC

AAAATCTCAT

GGGGCTATTA

TTCCGCAGCG

CACCAGTACA

GAAGACCTAT

CAAGGAAATT

ACCGCTCAAT

GGGTGCCGAT

GCAAGGACGT

GCATAACCGT

TGTGAACCTC

TACAGCAT

AAAATTGTTA

AATGGACTAT

ATTTCTCTCC

AGTGTCGTGA

TCAAAAAATA

ACCCG'ITCGG

GAAGTGGGTIG

TI'ACAAGATA

ATCGCTGTTC

ATTAATCTC

ACGGAGATT

AGGA.AAGGAA

CCATTCCATT

GTCAA.ACGGT

TAGCTCAAAC

CGCTTITAGA

CTACTTATCA

ACAATAATGA

AAAACAGCAT

TAGCGTATAT

GTTGAGCACA

ACTTGCTGCG

GACTACTCAA

AACATCAAAA

AGCAGCCA.AT

AGTGGATGAA

CATTIGAGATI'

TAGTGATAGT

GTTAAAACAT

TCCGGCAGCT

AGACATAGAT

TGAAGGGAAC

ATCCATTAAA

GGAAATTAGT

TACGGTGGCA ATGCG ITAAT 'TCGTTAGAAG ATOGACCAGA ATTGAGGCAG TGGGGTTACG ATGGAAPLATG GTGCGGATCA GCCAAT'CAAA AAGGTTATAC 119 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

AAAATTTTAG CACAGTACAA CTAA EF040-2 (SEQ ID NO:154) M NKKILMGLLS VVTIPLLAAC QGGETPSAAS I<NSQTVTTQS SAKTESTS IT RSVAQTTSKE EVKEPMKlTYE VGALLEAANQ RDTKKVKEIL QDTTYQVDEV OTEGNTPLNI AVHNNDIEIA KALIORGADI NLQNSISDSP YLYAGAQGRT EILAYMLKHA TPDLNKHNRY GGNALIPAAE KGHIDNVKLL LEDGREDIDF QNDFGYTALI EAVGLREGNQ LYQDIVKLLM ENGADQSIKD NSGRTAMDYA NQKGYTEISK ILAQYN EF040-3 (SEQ ID NO:155) AGCG TCAAAAAATA GTCAAACGGT GACTACTCAA

AGTAGTGCAA

CAGGAAGTGA

CAACGAGATA

GTCGACACAG

GCAAAAGCGT

CCCTATCTTT

GCGACCCCAG

GAAAAAGGAC

TTCCAAAATG

CAACTTETACC

GACAAMTCTC

AAACTGAAAG

AAGAACCGAT

CGAAGAAGGT

AAGGCAACAC

TGATTGATCG

ATGCGGGAC

AITTAA.ATAA

ATATTGACAA

ACTTTGDCTA

AAGATATT1GT

GTCGAACAGC

CACCAGTACA

GAAGACCTAT

CAAGGAAATT

ACCGCTCAAT

G-GTGCCGAT

GCAAGGACGT

GCATAACCGT

TCTGAAGCTC

TACAGCATTG

AAAATTGTTA

AATGGACTAT

ACCCGTTCGG

GAAGTGG07r

TTACAAGATA

ATCGCTGTTC

ATTAATCTGC

ACGGAGATTT

TACGGTGGCA

TTGTTAGA-AG

ATTGAGGCAG

ATGGAAAATG

GCCAATCAAA

TAGC~tAAAC

CGCTTTTAGA

CTACTrATCA ACAATAkATGA

AAAACAGCAT

TAGCGTATAT

ATGCGTTAAT

ATGGACGAGA

TrCGGGTr-ACG

GTC-CGGATCA

AACGG=ATAC

AACATCAAAA

AGCAGCCAAT

AGT~rCATGAA

CATTGAGATT

TAGTGATAGT

GTTAAAACAT

TCCGGCAGCT

AGACATAGAT

TGAAGGGAAC

ATCCATTAA

GGAAA'AGT

0 0* 0 0 00 00 0 0 *0 *0 *0 0 000 0 00 0 0 000 0 AAAAT =TIAC CACACTACAA C EF040-4 (SEQ ID NO:156) AS KNSQTVTTQS SA.KTESTSTT RSVAQTTSKE EVKEPMX<TYE DTEGNTPLNI AVHNNDIEIA KALIDRGADI TPDLNKHNRY GGNALIPAAE KGHIDNVKLL LYQDIVKLLM ENGADQSIKD NSGRTAM~DYA

VGALLEAANQ

NLQNSISDSP

LEDGREDI DF NQKGYTEI SI RDTKKVKEI L

YLYAGAQGRT

QNDFGYTAL I

IL.AQYN

QDTTYQVDE=V

EILAYMLKHA

EAVGLREGNQ

0* 0000 0 0 000* 0000 0 0000 00 0 00 00 EF041-1 (SEQ ID NO:157)

TAATTATTAA

ATGAAA'ITGA

CCGGCT~rGTG

AGTGGCGAAC

CTAGCAACAG

TAGACAA.AG

GATGGACTAA

GTGACTGCTA

GAATATGCTT

GATAAATCAG

AAAGCAACAC

GACATTGTGG

GGTCCATTCG

AA.AAACGATC

GTGAAAGAAT

CTTTCTGGTG

GCATCAACAC

NTITCTGATIT

AAA.AGTCAT

GAGGCGGCGG

AAGTTTI'ACG

NCAGAAT'AG

ATAACAAAGT

CATACAAAAT

ATGACTATGT

ATCTGTATGC

AAI'IAGGAAT

CATACTITGA

AAAAATATGG

TCTTAGACGG

AATATTGGGA

CACCAACCGC

AATTAGCCCA

AATATATGGA

TTCAGAAAAT

AACATTCGGT

AACGTCAGAT

TGTCACAGAA

T'FrTATTGCA

CCAACCTGCA

TAAATTAAAT

TTACGGATGG

CTCTGTAAAA

TAAAGCAGTC

TTAC~rATTA

TAAAAAI'AT

CTTTGATGGT

TAAAGATACT

GTTGAACTTG

ACAAATGGCC

ACTAAATCAA

ACAGA=TCA

GTGATI'ACAT

AGCTCAAGCG

CAACAAGAAA

TTAAATAATrG

GGTGCAGCGG

AAAGATGCAA

CAACGAACAG

AATGGTGATrG

AGTGATACAG

GCTTTCCCAT

GCATCAAACA

CCTGGTACAG

GTGAAACTGG

TTCCAAGATG

TTAIT=TAGG

TATTTACT

CGTCTGGTGG

TGCCAACAC

TATATGAAGG

AAAAAGCAGA

AATGGTCAGA

TTGATCCAGC

CCA TGCTAA

AATTAGAAAT

CAITCTTrCC

GCGAAAGTGC

ATACAAAATG

ACTCAGTAGA

GACAAACAGA

AGGCAACACT

AAcAAcTI'TA

CGGTAAGGCA

TGATTTATCA

AATTTATCGT

AGTTTcTGAA

CGGTAAACCA

CACAGCTTCT

AGGGGAAAAA

CACTTTAGAA

GCAACGTCAA

TGTCTACAAT

GTCATTCAAG

TGTGAATGTC

CGATGTCGTT

AATGACCCAG CTI'TTG'ITAG TCAAAAAGAA CGTGATGAAM AATCACCATT TAG-AAATGCG 120 TABLE 1. Nucleotide and Amino Acid Scqeuences of E. faecalis Genes.

AACTTACGTA AAGCAATC TTACTCA-ATC GACCGTAAAC CGTTAGTTGA ATCAATCCTT

AGGGGATGG

EF041-2 (SEQ ID NO:158) M KLKKSLTFGV ITLFSVTI'LA ACGGGGTSDS SSASrCGGKAS GEQVLRVTEQ QEMPTADLSL ATXRISFIAL NNVYEGIYRL DKDNKIVQPAG GLTYKIKLNK DAXWSDGKPV TANDYVYGWQ RTVDPATASE YAYLYASVKN KSELCIKAVS OTELEITLEK ATPYFDYLLA FPSFFPQRQD IVE-KYG-kNA PFVLDGFDGP GTDTKWSFKK NDQYWDKDT KLOSVDVNVV KESPTALNLF SGELAQQMAN DPAFVSQKEA STQYMELNQR DEKSPFRNAN' LRKAISYS ID AAEKAE VS ED

GDAIAKGEKD

SNSESAVYNG

QDGQTDOVVL

RKALVESILR

EF041-3 (SEQ ID NO:159) TTGTG GAGGCGGCGG AACGTCAGAT AGCTCAAGCG CGTCTGGTGG CGGTAAGGCA 0 4 0* 000 *0 00 0 0. 0

AGTGGCGAAC

CTAGCAACAG

TTAGACAAAG

GATGGACTAA

GTGACTGCTA

GAATATGCTI'

GATAAATCAG

AAACCAACAC

GACAFI'GTGG

GGTCCATTCG

AAAAACGATC

GTG;AAAGAAT

CTTTCTGGTG

GCATCAACAC

AACTTACGTA

AGGGGATGG

AAGTTTTrACG

NCAGAATTAG

ATAACAAAGT

CATACAAAAT

ATCACT.ATGT

ATCTGTATGC

AATTAGGAAT

CATACTTTGA

AAAAATATGG

TCTTAGACGG

AATATTGGGA

CACCAACCGC

AATTAGCCCA

AATATATGGA

AAGCAATTTC

TGTCACAGAA

TTTTATTGCA

CCAACCTGCA

TAAATTAAAT

TTACGGATGG

CTCTiGTAAA.A

TAAAGCAGTC

TTACTTATTA

TAAAAA 1TAT

CTTTGATGGT

TAAAGATACT

GTTGAACTTIG

ACAAATGGCC

ACTAAATCAA

TTACTCAATC

CAACAAGAAA

TTAAATAATG

GGTGCAGCGG

AAAGATGCAA

CAACGAACAG

AATGGTGATG

AGTGATACAG

GCT='C CCAT

GCATCAA-ACA

CCGGTACAG

GTGAAACTGG

TTCCAAGATG

AATGACCCAG

CGTGATGAAA

GACCGTAA-AG

TGCCAACAGC

TATATGAACG

AAAAAGCAGA

AATGGTCAGA

TTWGATCCAGC

CCA=GCTAA

AATTAGAAAT

CATTC'TrCCC

G-CGAAAGTGC

ATACAAAATG

ACTCACTAGA

GACAAACAGA

CT'T7MITTAG AATCACCA 1T

CGTTAGTTGA

TGAI'TATCA

AATATCGT

AGTTTCTGAA

CGGTAAACCA

GACAGCTTCT

AGGGGA.AAAA

CACTTTAGAA

GCAACGTCAA

TGTCTACAAT

GTCATTCAAG

TGTGAATGTC

CGATGTCGTT

TCAAA.AAGAA

TAGAAATGCG

ATCAATCC T EF041-4 (SEQ ID NO:160) CGCGTSDS SSASGGGKAS GEQVLRVTEQ QEIMPTADLSL ATXRISFIAL GLTYKIKLNI( DAKWSEDGKPV TANDYVYGWQ KSELGIKAVS DTELEITLEK ATPYFDYLLA PFVLDGFDGP GTD)TKWSFKK NDQYWDKDIV SGELAQQMAII DPAFVSQKEA STQYMELNQR

GW

EF044-1 (SEQ ID NO:161) NNVYEG IYRL

RTVDPATASE

FPSFFPQRQD

KLDSVDVNVV

DEKSPFRNAN

DKDNI<VQPAG

YAYLYASVKN

IVEKYGKNYA

KES PTALNLF

LRKAISYSID

AAEKAEVSED

GDAIAKGEKD

SNSESAVYNG

QDGQTDDVVL

RKALVES ILR

TAAGATAAAA

ATTI'TAGTTA

GAAAATTCTA

ATAGAAACAA

ACrCGTCAA

GCAACACCTT

GGAATCAATG

rrCCGAACTA

TI'AGTTATAG

TrTTTTTAAC

CCAATCATAC

GCTCTAATAA

AGTCGAAAAA

ATGCTCGTCGA

TGCCAACTI'C

AATTGTrTTGG

CGTCTATAGG

AGG=TTAGT

AACATCAATA

TAAACTAAAA

TGAAGTATCT

TCTrAGTAGC

AATI'ACGTTA

GGCTGAAA.AT

AGGAATAGTA

TCTCAAAAAG

AAAAGCAGTA

GAAACTTCAG

ACAAATGTCG

TTAAACAATC

GAGAACTTAA

GGTCAAGTGA

TGAAkAAAATT CGAATGAkACC

CTAATCATTA

AAAGTGCCAG

AAGAAGCAAA

CACTCGTATT

ATTCAACACC

AAGAAGCCAT

AGTTTGTGTT

TAAA.AAACAA

CAGTTCTAC

CACCACTCAA

TTCTrAGAA

TAATITTAA-A

AACTGCTACC

TAATAAATAT

121 TABLE 1. Nucleotide and Ami'no Acid Seqeuences of E. faecalis Genes.

GAGCTATCTA TAAATACAAT TCCTACAAAA GAGATTAGAA TATTrCAGC GGCCGATAAC AGTATTCGCA CCGTTAAAGT AAATACAGAA TTAATTTAG GAACTAATAT TTCTTCAAAC GATGAACAAA ATAGATCGGG CACTTTATAC TTA'ITCAACA ATAAAAATGG TI'CGATATCT TTAATCACTC CTAACTACGC TGGCAATGTT ACGGATG-ATC AAAAAGACGT TATGCTAGA.A GTAATTCAAT AA EF044-2 (SEQ ID NO:162) MrKKLVCVI L\JIFLTCCSS QKANEPKXQE NSTNHTTSIK SSTNHYSSSI ETSSNNKLKE TSESASTTQT SSKSKNEVST NVEEANSLEA TPYA'JDLSSL NNPLVFNFKG MNVPTSITLE NLNSTPTATF RTXLFGAENG QVKE-AINKYE LSINTIPTKE IRIFSAADNS IRTVKVNTEL ILGTNISSND EQNRSGTLYL FNN~kNGSISL ITPNYAGNVT DDQKDVMLEV

IQ

EF044-3 (SEQ ID NO:3.63) ITGTAGT TCTCAAAAAG CGAATGAACC TAAAAAACAA

GA.AAATI'CTA

ATAGA.AACAA

ACTTCGTCAA

GCAACACCTT

GGAATGAATG

TTCCGAACTA

GAGCTATCTA

AGTATTCGCA

GATGAACAAA

TTAATCACTC

GTAATTCAA

CCAATCATAC

GCTCTAATAA

AGTCGAAAAA

ATGCTGTCGA

TGCCAAC'ITC

AATTGTTTGG

TAAATACAAT

CCGTTAAAGT

ATAGATCGGG

CTAACTACGC

AACATCAATA

TAAACTAAAA

TGAAGTATCT

TCTTAGTAGC

AATTACG ITA

GGCTGAAAAT

TCCTACAAAA

AAATACAGAA

CACTTATAC

TGGCAATGTT

AAAAGCAGTA

GAAACTTCAG

ACAAATGTCG

TTAAACAATC

GAGAACTALA

GGTCAAGTGA

GAGATTAGAA

TTAATTTTAG

TTATTCAACA

ACGGATGAT.C

CTAATCATTA

AAAGTGCCAG

AAGAAGCAAA

CACTCGTAT

ATTCAACACC

AAGAAGCCAT

TATTTTCAGC

GA.ACTAATAT

ATAAAAATGG

AAAAACACGT

CAGTTCTAGC

CACCACTCAA

TTCTTTAGAA

TAATrTITAAA

AACTGCTACC

TAATAAATAT

GGCCGATAAC

TTCTTCAAAC

TTCGATATCT

TATGCTAGAA

EF044-4 (SEQ ID NO:164) CSS QKANEPKKQE NSTNHTTSIK SSTNHYSSSI ETSSNNKLKE TSESASTTQT SSKSKNEVST NVEEANS LEA MNVPTS ITLE NLNSTPTATF RTKLFGAENG QVKEAINXYE IRTVKVNTEL ILGTNISSND EQNRSGTLYL FNNKNGSISL

IQ

TPYAVDLSSL

LSINTIPTKE

ITPNYAGNVT

NNPLVFNFKG

IRIFSAADNS

DDQKDVMLEV

4 EFO4S-1 (SEQ ID NO:165)

TAGCCAAAAA

TTAGCTTCTG

AATAAACCAA

AAAGCAATTG

C ITTTCCAGC

CACCCATTAT

AAATTAGATC

GACGGTAAAG

GACTATACAG

CATGATGGTA

ATCACTTATA

GTTTTACCAA

GTTCGTAAAA

TCTGI'rGAAT

ATAGGGAGG

TGTTAGTATT

CGGAAACAGA

ATGGCGGTAC

AAGAATTTTA

TTAACAATGA

AAGATCCCAA

ATGTGACAGC

GGATTCGITA

AATCGCCAAC

AAGAAGTTCA

AACATrCCTT

ACCCTCTGAC

ACCTACCAAA

AAAAGAGATG

CGGGTTAGCT

AGACAT7I'CA

ATI'AGATGTC

TCAAGACAAC

TGCAGACT

TACAGCAACC

CGATGACGTG

TGATGATAAC

cAITTCTGGC

CCCAGGAATG

TGAAGGAAT

TATTGGACCA

TGAGCATTAC

AACAAGAAAC

GCATGTGGTG

AAAATGCCAA

GCTGTACTTA

TATGATG-CAC

AAGATTGTCG

ATTAATTAC

ATITI'CTC'T

T~rACGA-ATA

ATAGAAAMAG

CAACAATTAG

GCTGTTAAAG

TACTACATGA

TACGGTGGTA

GGATTI'TAGG

GccGGcAATAA

TCGCTGTI'A)

TGGATACACA

AATACATGCT

ATGGGGGTCC

GTGACAATTT

ATGAAGTCAT

TTGTTGGCAT

TCAATCATAA

GTGGCGGTGT

ACATGGAATC

GTAATATTGT

AACCTAAA-T

TGCAATCACG

AGGCGGGGGC

AAATGATAAA

ATTCCAAGGA

TCCAACGGTA

TGCGGATCTG

GAAATGGTCT

TGGTCATA;A

GGAAGACTAC

AGAAGTTAAA

TrGGGGCTCA

AAGCGATGCA

GACAGGTGAA

AGATAAATTA

122 TABLE 1. Nucleotide and Amino Acid Seqeueiices of E.fcaecalis Genes.

GTGTTCAAAT

ATTCCATTAT

ATCTTAGGAC

AAAGAAACAA

CAAGCCATGG

CGAACAGGGG

AAAGGCTATA

GACGTAGACG

GCTTCAATGT

AAAGAAATTG

TATGATAAAT

GGTTCAGATC

TTTGAGTCAG

GAAGAAAAAC

GTA.ATCCCAA

ACTTGGGCAG

CTGTTCCTTC

CAATGCCAAC

GTCCCGAACA

ATACAGTGAA

CCTATGCAAT

CAACAACGTT

CGCTTGA=I

GCGATGGCAT

CAGGCGGCGA

GCTTAAACGT

TGAAAAATGA

CTTCACCAAC

AAGAAAATAC

GTAAAAAAGC

CCCTTTACAG

TTGATACGAA

TGCGAGCATT

AGATACGTAT

AGCCTACACG

ATACAATCCA

TGACAATCAT

AATCCCACCA

AGACAAAGCG

TCGCGAAGAC

PLACTGCACA.A

AACGTATACA

TGACCCAGAA

CGGCTrATAT

TAAATTACTT

CTTCTACGAT

AAATGAAGTC

AGATAATCCA

GTAGAAGCGA TGAAAGCGAA CCAACATACA AAGATACTGA TATATTGG;CT TTAAAATCG AAAGCTAAAA TGGCAGATAA GCAGTCGGCC AAAAATTCTA GTCTTCAAGA

GCTTGCATGA

AAAAAATTAT TAGACGATGC AAAGAAGGCA AACCACTAGA CCACTTGCTO.

ATTACTATT

ACAGGACGCT TAATTG-AT=t GTAGATATCT

ATCAAGGCGC

_GjtCCAACT CACCCTIAA GATGCGATTG ATTCA.AAGC TGGCAAGAGT ATGCCAkTTGA rI'CCCTGTCA

ACGACCGTGT

TGGG~cAACG-G TGGGTfGTCAC

ACAATACGAT

AGGGTATCAA

TACGTTGAC

AAGCTTACGT

CAACGGCTTA

TAGCGAAGCG

TGGTTATAAA

AATCAAGTTT

CCAACAATGG

CCAAGCATTC

GTGGGGCACA

CTATACACGT

ATCATTAT

TGAAGCGT

AGTTGACTTT

AGCAGACTCA

4

CGGAAATAA

EF045-2 (SEQ ID NO:166) MN KKRILGAITL ASVLVFGLAA CGGGNKGGGN KATETEDISK MPIAVKND AIDfGTLDVA

LDEDANTATI

DGKSPTISGI

RKNPVTIGPY

ALSMPTDTYP

AMGYAIDNDA

VDGDGIREDK

DKLKNEDDPEV

E:KRKKAFYDW

VVMDTQFQGL

KLRONLKWSD

EKVNDKEVK I

YMSNIVTGES

TYKDTEGYQI

VGQKFYNGLR

EGKPLEIKFA

DIYQGAWGTG

QEYAIDEAFV

FQQEFYQDNY

GKDVTADDVI

TYKEVHPGMQ

'JEYLPNE}{YY

LGRPEQAYTY

TGATTLIPPV

SMSGGETAQP

SDPSPTGLYG

IPTLYRNEVL

DAQYMLPTVQ

FSYEVIGHKD

QLGGGVWGSV

GGKPKLDKLV

IGFKMGTFOK

FKSLHDSEAY%

LADYYVQQWKI

PNSAFNYTRF

PVNDRVVDFT

YTG IRYDONF

LPKHAFEGIA

FKSVPSASIV

ETNTVKYNPK

GYTLDLDKAK

EIGLNV'TYTT

ESEENTKLLD

WAVDTKDNPW

XK

IVDGGPADLK

TNIVGMEDYH

VKDMESSDAV

EAMKAKQYDI

AKMADKSLRQ

KLLDDAGYKD

GRLIDFQAFY

AIDSKASFDE

ATVGVTADSR

K

EF045-3 (SEQ ID NO:167) ATGTGGTG GCGGCAATAA ACGCGGGGC

AATAAAGCAA

AAAGCAATTG

CT'ITTCCAGC

CAGCCATTAT

AAATTAGATG

GACGGTAALAG

GACTATACAG

CATOATOCTA

ATCACTTATA

GTTTACCAA

GTTCGTAAAA

TCTGTTGAAT

GTGTTCAAAT

ATTOCATTAT

ATC ITAGCAC

AAAGAAACAA

CAAGCCATGG

CGAACAGGGG

AAAGGCTATA

CGGAAACAGA

ATGGCGGTAC

AAGAATTITA

TTAACAATGA

AAGATGCCAA

ATGTGACAC

GGATTCGTTA

AATCGCCAAC

AAGAAGTTCA

AACATGCCTT

ACCCTGTGAC

ACCTACCAAA

CTGTTrCr

CAATGCCAAC

GTCCCGAACA

ATACAGTGAA

GCTATGCAAT

CAACAACGTI-

CGC=TATTI

A.GACA=TCA

ATTAGATGTC

TCAAGACAAC

TGCAGACTTT

TACAGCAACC

CGATCACGTG

TGATGATAAC

CATTTCTGGC

CCCAGGAATG

TGAAGGAATT

TATTGGACCA

TGAGCATTAC

TGCGAGCATT

AGATACGTAT

AGCCTACACG

ATACAATCCA

TGACAATGAT

AATCCCACCA

AGACAAAGCG

AAAATGCCAA

GCTGTAGTTA

TATGATGCAC

AAGATTGTCG

ATTAAATAC

ATTTTCTCTT

TTTACGAATA

ATAGAAAAAG

CAACAATITAG

GCTGTTAAAG

TACTACATGA

TACGGTGGTA

GTAGAAGCGA

CCAACATACA

TATATTGGCT

AAAGCTAAAA

GCAGTCGGCC

GTCTTCAAGA

AAAAAATTAT

TCGCTG'ITAA

TGGATACACA

AATACATIGCT

ATGGGGGTCC

GTGACAATTT

ATGAAGTCAT

T'rGTTGG.CAT

TCAATGATAA

GTGGCGGTGT

ACATGGAATC

GTAATATrGT

AACCTAAAT'

TGAAAGCGAA

AAGATACTGA

TTAAAATGGG

TGGCACGATIA

AAAAATTCTA

GCTTGCATGA

TAGACGATGC

AAATGATAAA

ATTCCAAGGA

TCCAACGGTA

TGCGGATCTG

GAAATGGTCT

TGGTCATAAA

GGAAGACTAC

AGAAGTTAAA

7TrGGGGCTCA

AAGCGATGCA

GACAGGTGAA

AGATAAATTA

ACAATACGAT

AGGGTATCAA

TACGTTTGAC

AAGCTTACGT

cAAcGcTTA

TAGCGAAGCG

TGGTTATAAA

123 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

GACGTAGACG

GCTTCAATGT

AAAGAAAITG

TATGATAAAT

GGTTCAGATC

Tm'TGAGTCAG

GAAGAAAAAC

GTAATCCCAA

ACTTGGGCAG

CGGAAA

GCGATGGCAT

CAGGCGGCGA

GCTTWACGT

TGAAA.AATGA

CTTCACCAAC

AAGAAAATAC

GTAAAAAAGC

CGCTTACAG

TTGATACGAA

TCGCGAAGAC

AACTGCACAA

AACGTATACA

TGACCCAGAA

CGGCTTATAT

TAAATTACTT

CTTCTACGAT

AAATGAAGTC

AGATAATCCA

AAAGAAGGCA AACCACTAGA CCACT1'GCTG ATTACTATGT ACAGGACGCT TAANTTGATrT GTAGATATCT ATCA-AGGCG-C GGTCCAA.ACT CAGCCTTPA GATGCGATTG ATTCAAAAGC TGGCAAGAGT

ATGCCATTGA

TTCCCTCTCA ACGACCGTCT TGGGCAACGG

TGGGTGTCAC

AATCAAGTTT

CCAACAATGG

CCAAGCATTC

GTGGGCACA

CTATACACGT

ATCATTTGAT

TGAAGCGTT

AGTTrGAC=F

AGCAGACTCA

EFO4S-4 (SEQ ID NO:168)

CGGGNKGGGN

Al DGGTLDVA

LDEDANTATI

DGKSPTISGI

RKNPVTIGPY

ALSMPTDTYP

AMGYAIDNDA

VDGDGIREDI(

DKLA(NODPEV

EKRKKAFYDW

KATETEDISK

VVMDTQFQGL

KLRDNLKWSD

EKVNDKEVKI

YMSNIVTGES

TYKDTEGYQI

VGQKFYNGLR

EGKPLEIKFA

D IYQGAWGTG

QEYAIDEAFV

MP IAVKNDKK

FQQEFYQDNY

GKDVTADDVI

rYKEVHPGMQ

VEYLPNEHYY

LORPEQAYTY

TCATTLIPPV

SMSGGETAQP

SDPSPTGLYG

IPTLYRNEVL

DAQYMLPTVQ

FSYEV IORKO

QL.GGGVWGSV

GGKPKLDKLV

IC FKMGTF OK

FKSLHDSEA-K

LADYYVQQWK

PNSAFNYTRF

PVNDRVVDFT

PLFNNDADFK IVOGGPADLK YTr I RYDD)NF TNIVGMEDYH LPKHAFEGIA

VKDMESSDAV

FKSVPSASIV EAi'ThAKQYDI ETNTVKYNPK

AKMADKSLRQ

GY'TLDLDKAK

KLLDDAGYKD

EIGLNVTYTT

GRLIDFQAFY

ESEENTKLLD AIDSKASFDE WAVDTKONPW ATSJCVTADSR EF046-1. (SEQ ID NO:169) 0 0

TACCAGGATA

TGTAGTA.ATA

CAGCAAACTA

ACATCATCTA

GATGATGTTT

ACGGATAAAG

AATTATATTA

TAATGAAAAA

ATACTGGGGG

CCCAGTCTTC

TAACAATTGA

CAAAAACTAG

AACTAAAGGA

AGCAAAAA

AAAACTTA T

AAAAAATAGC

TAAAAAAGAT

AACAACCGAG

ACGACAATTG

ATATATATCA

GTACTATIGT TAGCCTTA GACGCTTCAT CTACTGAAGT AGTACTAATC CGGACACAAC AATTTAAAGA ATAGAGAATT TATGAACA.AG GAATTAACAG GAGGCTAAAG AACAAAAGAA

TTTAACGGCA

ATCAACTAAG

ACCAACTTCT

GAATCCAACA

TTCAACAATT

AGATGTCATT

EF046-2 (SEQ ID NO:170) MKKKLIV LLLALFLTAC SNNTGGKNSD ASSTEVSTKQ QgQSSKYDS

SNFDTTPTST

SSITIETTEN LKNRELNPTD DVSKTRRQLY EQGINSSTIT DKELKEYISE AXEQKKDVIN

YIKQK

EF046-3 (SEQ ID NO:171)

A

TGTACTAATA ATACTGGGGG CACCAAACTA CCCAGTCTI'C ACATCATCTA TAAcAATTGA GATGATGTI'T CAAAAACTAG ACGGATAAAG AACTAAAGGA AATTATATTA AGCAAAAA

AA.AAAATAGC

TAAAAAAGAT

AACAACCGAG

ACGACAATTG

ATATATATCA

GACGCTTCAT CTACTGAAGT AGTAGTAATC

CGGACACAAC

AATTTAAACA ATAGAGATT TATGAACAAG GAATTAACAG GAGGCTAAAG AACA.AAAGAA

ATCAACTAAG

ACcAAcTTcT

GAATCCAACA

TTCAACAATT

AGATGTCATT

124 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

EF046-4 (SEQ ID NO:172) C SNNTGGKNSD ASSTEVSTXQ QTTQSSKKDS SNPDTTPTST SS ITIETTEN LKNRELNPTD DVSKTRRQLY EQG INS STIT DKELKEY ISE

YIKQK

AKEQKKDVIN

EF047-1. (SEQ ID NO:173)

TAGGGAAAC

FI'AACCCTTG

TCCAGTGCTG

AGTAGCAAAG

TCGAAAGCAA

TCACCAGCGA

AACATrGTTTC TTAkACTGCAG

GAAAAAGAAG

T'IGAGAAAA

GACAATGGCG

GCCGTTCTA

AATATCAATG

GAAACATTAC

GACTATAATC

TTTGACCCA.A

AAGGAGGAAT

CTTTAGCAGC

CGGAAACTAC

CAACAACAAA

CAGCTI'CTTC

AAAATGCGAA

CTGGTCAAG

CGACAACTTC

CGATTCCAGT

AAACATATG

GTCAACCAGT

GTI'ACITATC

GTCAATCGCC

CAGCACCGAA

GAAACTCAGT

TTCAAGCAGT

TCTTATGAAA

ATGCGGCGGC

CACAGTCGAG

ATCTAGTGAT

TACGAAGGAA

TCCAGATGAC

CTTACCGCAG

ACAAGCGGAT

GAATGATGCA

CTCTGATGCC

AGATITAGGT

TTGGCAAGAA

TGATGAT'ITA

TACCGTTGGT

AGTTTGGCAA

GAAGATGGCA

AAGATAGGGC TTP GGAAAAAGTA CAC AGTACAAAkAG CAI GCGAAACCGT CAC GCGGCAAATA ATC CAAGCCAACC AAC GCAATrTTAA CGI CAAAACAATI TC( CGTGTCAATC AG~ G.AAGCAAAAA AT( TACA.ATATTA CT( GGCAATTGGA GT GCGAAAAATG Tr( CAAATCACAC TG~ GCCGGTACAG TCC ACATCAATGT AA

;AAAATAC

'CTGCTAC

;GA-ACA.AC

;GcTCAGC

TGCTITAA

.GTCAAAC

:GTGT=~

ETAACGCC

~CACTrCAA ;GG 'ATAA rTAGTCGT

:;TCAACAT

A.ACGTGGC

GT7TTACTC

GGATAGTCGT

AAALAGAATCA

AACAGCTGAT

AGAGAAGCAA

CCAGCTAGCA

GAATAACTT

ATATTATGCA

AATTAG'ITCT

CCAAATCAT

ACAAGGGGCG

ACGGGCCTCA

TTTGGAACAA

AGGAACCACT

TGTCCATCAT

TCTAG TGCITTCTT EF047-2 (SEQ ID NO:174) MKK IGLISSAFLL TLALAACGGG KSTF.NTDSRS SAAESTTVES TKASATKESS SKATTKSSDA KPSGTIADS KATASSTKEA ANNGSAEKQS PAKNANPDDQ ANQVLNQLAN MFPGQGLPQA ILTSQTNNFL TAATTSQADQ NNFRVLYYAE KEAIPVNDAR VNQLTPISSF EKKTYGSDAE AKNAVNQIID NGGQPVDLGY NITGYKQGAA GSSYLSWQEG NWSLVVRASN INGESPDDLA KNVVNILEQE TLPAPNT-VGQ ITLNVAGTTD YNRNSVVWQA GTVVYSVHHF DPIQAVKMAT SM EF047-3 (SEQ ID NO:175)

ATGCGGCGGC

TCCAGTGCTG

AGTAGCAAAG

TCGAAACCAA

TCACCAGCGA

AACATGTITC

TTAACTGCAG

GAAAAAGAAG

TI'TGAGAAAA

GACAATGGCG

GCAGGTTCTA

AATATCAATG

GAAACATTAC

GACTATAATC

'rITGACCCAA

GGAAAAAGTA

CGGAAAGTAC

CAACAACAAA

CAGCTI'CTTC

AAAATGCGAA

CTGGTCAAGG

CGACAACTTC

CGAT1'CCAGT

AAACATATGG

GTCAACCAGT

GTTACTTATC

GTGA.ATCGCC

CAGCACCGAA

GAAACTCAGT

TTCAAGCAGT

CAGAAAATAC

CACAGTCGAG

ATCTAGTGAT

TACGAAGGAA

TCCAGATGAC

CTTACCGCAG

ACAAGCGCAT

GAATfGATGCA

CTCTGATGCC

AGATTTAGGT

TTGGCAAGAA

TGATGATTA

TACCGTTGCT

GGATAGTCGT

AGTACAAAAG

GCGAAACCGT

GCGGCAAATA

CAAGCCAACC

GCAATTTTAA

CAAAACAATT

CGTGTCAkATC

CATCTGCTAC

CAGGAACAAC

ATGCTCACC

AAGTGCI'TA.A

CGAGTCAAAC

TCCGTGTTTT

AGTTAACCCC

AAAAGAATCA

AACAGCTGAT

AGAGAACCAA

CCAGCTAGCA

GAATAACTTT

ATATTATGCA

AATTAGTTCT

CCAAATCATT

ACAAGGGGCG

ACOGGCCTCA

TTTGGAACAA

AGGAACCACT

TGTCCATCAT

GAAGCAAAAA ATGCAG GAA TACAATATTA CTGGGTATAA GGCAATTGGA GTTTAGTCGT GCGAAAAATG TTGTCAACAT CAAATCACAC TGAACGTGGC AGTTICGCAA GCCGGTACAG TCGT'TrACTC GAAGATGGCA ACATCAATGT AA EF047-4 (SEQ ID NO:176) 125 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

CGO KSTENTDSRS SAAESTTVES TKASATKESS SKATTKSSOA KPSGTTI'ADS KATASSTY<EA ANNGSAEI(QS MFPGQGLPQA ILTSQTNNFL TAATTSQADQ NNFRVLYYAE EK:-TYGSDAE AXNAVNQIIED NGGQPVDLGY NITGYKQGAA INGESPDDLA KNVVNIELEQE TLPAPNTVGQ ITLNVAGTTD DPIQAVKMAT SM

PAKNANPDDQ

KEA IPVNDAR

GSSYLSWQEG

YNRNSVVWQA

ANQSJLNQLAN

VNQLTPISSF

NWSLVVRASN

G;TVVYSVHHF

EF048-1 (SEQ I0 NC:177)

TAAGGAGAAA

GGATTACAGT

TTGCGCTATG

GAATTAGTAG

TTAGGAGGAG

GTCAGTGGAT

ATTTIGATA

GTCTATGATT

CGAAGTTT

ATTCGGGTCA

GTACCTATGG

GAGAATAATG

TATGATGAGC

TTGACAGCGA

AAAACGGTCT

GTTGTiGTTCA

TCATTAAAA

AGTTCATGAA

TGCTAAGTGG

CGTATGCTAG

AGGAAA.AAAC

AAACAGAACT

CAGCATTAGA

ATGAAAAACA

CTACAAAAAA

ATATGAGCAA

TGCAAAGTGA

GTAGTTCGGA

AGTTCGTI'1T

ATACTCGAGT

AACAAGAACA

TTAAAGAAC

ATCAAGTAGA

ATAGCTCAGA

AAAAAGAAAG

T'TGCGGCAAA

TAATAGCCAA

AGATGGTAAA

AATTAATTA

APAG=T7CT TrTTTTAAA

ATTAGGATTT

AGGGCCTGTT

AACCGCCATC

AGTATATACT

ATATACAGCT

GCCAGATATT

AGCGATTGAA

GGTTGAAGAA

CAGTGAACCA

ACATGGCGAA

G~tTTATTTA

ACAGAAGCTT

CCAGTTATCG

GTTCAAATTG

ACACAAACAG

AAAGATTATIT

GTAATGGATA

GTTGGTTTAA

ACATCTCCAG

AAAATGGTAG

TCTCTACA.AT

GGTCATGGTG

GTGATTATGA

GAAGCAGCAA

GAAAAGAAAA

TTCCAAAAAC

CTGTATCAGG

CAGCAGTTAT

CGGCA AATGA ATITC TATGAA

AATA=TCC

GTGCAAT-TGA

CTGTAT-ITtC

ATCA.AGCGCT

CTATTATGA

ATGA=FTGAA

AACT=TAGG

CTAATCTAAT

GTGTrGGCTAA

ACGACGGGAAC

AAGAATCGAC

AAGCACAAC

TTGTTCAACC

CTATTCGCCA

GGTATTGGCCA

TACGGTAGTC

GAAATTCGGT

AGATGGTCAA

TTTTGCA.AAG

CATTCCGTAT

AATGCAACCA

CTCTGGTCAA

AGGTAAAAAA

GGGTTCGCCA

CAACGCTGCA

GTATTATTCT

AAAAGAACGT

CGCTTTTGA

AGAATATGGC

GTTGCATGAA

GTTGGCGGAC

EF048-2 (SEQ ID NO:178) MKKRKV LFTAVMVLAG LQLLSGCGKT E-ASANDTVVL RYAYASNSQP VIDSMKKFGE LVEEKTDGKV Q IEYFPDGQL GGETELIELT QTGAIDFAXV SGSALESFSK DYSVFAI PY I FDNEKHFFXV MDNQAL.MQPV YDSTKXLGFV GLTYYDSGQR SFYMSKGPVT SPDDLKGKKI RVMQSETAIK MVELLGGSPV PMGSSEVYTS LQSNLINGAE NNEFVLYTAG HGGVAKYYSY DEHTRVPDIV IMNEGTKERL TAKQEQAIEE AAKESTAFEI( T\TFKEAVEEE KKKAQAEYGV VFNQVDSEPF QKLVQPLHES FKNSSEHGEL YQAIRQLAD EF048-3 (SEQ ID NO:179)

TTGCGGCAAA

TTGCGCTATG

GAATI'AGTAG

TI'AGGAGGAG

GTCAGTGGAT

ATTITTTGATA

GTGTATGATI'

CGAAGTTTT

ATCGGGTCA

GTACCTATGG

GAGAATAATG

TATGATGAGC

ETGACAGCGA

ACAGA.AGCTT

CGTATGCTAG

AGGAAAAAAC

AAACAGAACT

CAGCATTAGA

ATGAAAAACA

CTACAAAAAA

ATATGAGCAA

TGCAAAGTGA

GTAGTrCGGA

AGTTCGTTTT

ATACTCGAGT

AACAAGAACA

CGGCAAATGA

TAATAGCCAA

AGATGGTAAA

AATTGAATTA

AAGTTTT'TCT

TTTrTTrTAAA

ATTAGGATTT

AGGGCCTGTT

AACCGCCATC

AGTATATACT

ATATACAGCT

GCCAGATATT

AGCGATTGAA

TACGGTAGTC

CCAGTTATCG ATTCTATIGAA GTTCAAATTG AATAT'rTrCC ACACAAACAG GTGCAATIGA AAAGAWIATT CTGTA7MI'C GTAATGGATA ATCAAGCGCT GTTGGTIrTAA

ACATCTCCAG

AAAATGGTAG

TCTCTACAAT

GGTCATGG TG

GTGATTATGA

CTTA'ITATGA

ATGATTI'GAA

AACTF1=AGG

CTAATCTAAT

GTGTG.GCTAA

ACGAGGGAAC

GAAA'FTCGGT

AGATGGTCAA

TTTTGCAAAG

CATTCCGTAT

AATGCAACCA

CTCTGGTCAA

AGGTAAAAAA

GGGTTCGCCA

CAACGCTGCA

GTATrATTCT

AAAAGAACGT

GAAGCAGCAA AAGAATCr.AC CGCITTIGAA 126 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. fuecalis Genes.

AAAACGGTCT TrAAAGAAGC GGTTGAAGAA GAAAAGAAAA AAGCACAAGC AGAATATGGC G=TGT TCA ATCA-AGTAGA CAGTGAACCA TTCCAAAAAC TTGTTCAACC GTTGCATGAA TCA'PrCAAAA ATAGCTCAGA ACATGGCGAA CTGTATCAGG CTAT-TCCCCA GTTGGCGGAC

TAA

EF048-4 (SEQ ID NO:180) CGKT EASAN1TVVL RYAYASNSQP VIDSMICKGFGE LVEEKTDGKV QIEYFPDGQL GGETELIELT QTGAIDFAXV FONEKHFFKV MDNQALMQPV YDSTKKLGFV GLTYYDSGQR RVMQSETAIK MVELLGGSPV PMGSSEVYTS LQSNLINGAE DEHTRVPDIV IMNhEGTKERL TAKQEQAIEE AAXESTAFEK VFNQVDSEPF QKLVQPLHES FKNSSEHGEL YQAIRQLAD

SGSALESFSK

SFY-MSKGPVT

NNEFVLYTAG

TVFKEAVEEZ

DYSVFAIPYI

S?DDLKGKKI

HGGVAKYYSY

KKKAQAEYGV

EF049-1 (SEQ ID NO:181)

TGAGACTCT

AAAACAGGAG

GCCGTTrTA

TCAACAATTA

GCG'I'AGCC

GACAAAGANA

AAGTTCTCTG

AAACA.AAGAG

TTAAAA.ACAG

TCAGAAGATG

ATTGCTAAAG

GAI'CACAAG

GGCGA.AGTGT

GTAGTGAAAA

ATCAAGAAA.A

AGTGACGAAT

GCAGCCTACA

AGTGATTCTA

AGCAAAACAA

TCTTTT'CAA

TGCATAAGAG

GTTTAGCAGC

CTGTTGATGA

AAATCCGTTAT

TTCAAAAAAA

ATGCATTAAA

CAGCCTATGA

C'ITGGGCAAG

ATGCCAAAGC

AAAAATCAAC

CAACA.ACTGT

CAGAACCAAT

TGACCAAAAA

TTGCTGAAGA

TAAAAGCGGC

TrGCAAACTGA

AA.ACAAGCGA

CAGAATCTTC

AATGAGGTAT

AATGAAGAAA

GTGTTCAAGC

TTTTTATAAC

TTATAAAGTC

C=TGACGAA

ACAAGCTrGGT

TGCAGGTCTA

=rCCATCCA

TGTCAAGAAA

ACATACTGCT

TCCTGCCGAA

TGCTGCAACA

CAAAGCAAAA

AACAAAATTA

CAATGTGAALA

ATCTTCAAGC

TACCAAAACA

ICTAAATAA

GGTATAGTTA

AAACTAATCT

GGTTCAAAAG

CAAATTAAAG

TTTGAAGAAA

GCCAAAGAAC

TTAACTGAAA

AAACCCCACT

GAAGTAGAAG

GAAATCACTG

ACGAAAAAAG

GTI'AAAGAAG

AATATGCAAA

GCCAATGACA

GCCGATCAAA

ATTAAMGATG

GCTTCTTCAG

AGCGACTCTG

TAACAGANAT

TAGCTGCAC

ATATCGCAAC

AACAAAGCAC

AATATGCCGA

AAGTAGAAC

AAACATTCAA

TAAAAATTAC

CACAAATTAT

ACGGCGGCGA

ATGGCCTAA

CTGCCTTCAA

CCTACCAAAC

TGAAACC ITA CATTrTTTC

ATGCCT'CAA

AGAAAAAAGA

AAAAAGCAAC

AAAACTANAA

GGGCGCAATG

AATGAAAGGT

TAGCCAACAA

CAAAGTAACT

ACAAGGCGGA

GAAACAGTTA

AGATGAAGAC

CCAAGTTGCT

TTTCACAAAA

AATTAAATTT

ATTAAAAGAT

AACCTACTAT

TGAAAAAGAG

GAAAGTCATT

GAACGC=TA

ATCAAAATCA

AGATI'CTTCA

EF049-2 (SEQ ID NO:182) MKKK LILAAAGAMA VFSLAACSSG SKDIATMKGS TITVDDFYNQ IIEQSTSQQA FSQMVIYKVF EEKYGDKVTD FSDALKQAGL TEKTFKKQLI( QRAAYDAGLK AHLKITDEDL EDDAXAVKKE ITDGGDFTKI AI(EKSTDTAT KKDGGKIKFD EVSEPIAATN MQTYQITYYV VKMTKNKAXG NDMKPYEKEI DELKAANVKI KDDAFKNALA GYMQTESSSA SSEKKESYSS

KTTESSSK

KXIQKNFDE-A

KTAWASFHPE

SQATIPAEV

KKIAEET KLA

DSKTSDTKTS

KEQVEAQGGK

VEAQI IQVAS KEAkAFKLKDG DQTFVSKV IS

DSEKATDSSS

EF049-3 (SEQ ID NO:183)

GTGTTCAAGC

TCAACAATTA

GCGT~T AGCC

GACAAAGANA

AAGTTCTCTG

GGI'CAAAAG

CTGTTGATGA

AAATGGTTAT

TI'CAAAAAAA

ATGCATTAAA

ATATCGCAAC

TTTTTATAAC

TrATAA.AGTC

CITTTGACGAA

ACAACCTGGT

AATGAAAGGT

CAAATTAAAG

TTTGAAGAAA

GCCAAAGAAC

TTAACTG'AA

AACAA.AGCAC TAGCCAACAA AATATGCGA CAAAGTA.ACT AAGTAGAAGC ACAAGGCGGA AAACATTCAA GAAACAGTTA 127 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Gencs.

AA.ACAAAGAG

TTAAAAACAG

TCAGAAGATrG

ATTCTAA-AG

GATI'CACAAG

GGCGAAGTGT

CTAGTGAAAA

ATCAAGAAAA

AGTGACGAAT

GCAGGCTACA

AGTCA 1TCTA

AGCAAAACAA

CAGCCTATGA

CTTGGGCAAG

ATGCCAALAGC

AAAAATCAAC

CAACAACTGT

CAGAACCAAT

TGACGAAAAA

rGCTCAAGA

TAAAAGCGGC

TGCAAACTGA

AAACAAGCGA

CAGAATCTTC

TGCACGTCTA AAAGCCCACT T'FCCATCCA GAATAGAAG TGTCAAGAAA GAAATCACTG AGATACTGCT ACGAAAAAAG TCCTGCCGAA CrAAAGAAG TGCTGCAACA AATATGCAAA CAAAGCAAAA GGCAATGACA AACAAAATI'A GCCGATCAAA CAATGTGAAA AITAAAGATG ATCITCAAGC GCTTCTTCAG TACCAAAACA AGCGACTCTG

TTCTAAATAA

TAAAAATTAC

CACAAATTAT

ACG(GCGGCGA

AT-GGCGGTAA

CTCCTCA-A

CCTACCAAAC

TGAAACCTTA

CATTTr.TTTC

ATGCCITCA-A

AGAAAAAAGA

A.AAAAGCAAC

AGATGAAGAC

CCAAGTTGCT

TI'CACAAAA

AATTAAATTT

ATTAAAAGAT

AACCTACTAT

TGAAAAAGAG

GAAAGTCATT

GAACGCTTTA

ATCAAAATCA

AGATTCTTCA

EF049-4 (SEQ ID NO:2.84) a CSSG SKDIATHKGS TITVDDFYNQ IKEQSTSQQA FSDALKQAGL TEKTFKKQLK EDDAKAVKKE ITDGGDFTKI EVSEPIAATN MQTYQTTYYV DELA(AANVKI KDDAFKNALA

KTTESSSK

FSQMVIYK'JF

QRAAYDAGLK

AKEKSTDTAT

VKMTKWKAKG

GYMQTESSSA

EEKYGDKVTD

AHLKITDEDL

KKDGGK IKFD

NDMKPYEKEI

SSEKKESKSS

KX IQKNFDEA

KTAWASFHPE

SQATTVPAEV

KKIA=ETKLA

DSKTS=TTS

KEQVEAQGGK

VEAQI IQVAS

KEAAFKLKDG

D)QTFVSKVIS

DSEKATDSSS

EF050-1 (SEQ ID NO:185)

TAGGGTCTGG

AAAGGATGNA

CTTGTTCTAT

GCCACAAAAG

GGTTACGCAG

GCCACAACTC

GGAGCI'TAA

AACAATGAAT

TATCAAGTGA

TATI'TAGGAA

GGAGCACCTT

GAAAACGGCC

GTTCCAGAAA

GATGGAACGG

ACTCAAGTCA

AATCCTGAAG

AAAAGCAGTC

AAAAAATGAA

TATTAAGTGC

AAGCAACTGT

GAACTAAAAA

AAGAAI'AGT

GTGGCAAAGC

GGAATCAAAC

ACAAAGCCAA

CCTATGGGCA

TCAATCAT'T

CCACAGAAAA

AACTGCCCAT

TGCCGTTATC

CTAGCCAGAT

TAGATCAAT'

AACTGACT'1C

CATGCCCAAA

TTGCCAAATT

TCACTTAAAC

TTCGTTTGGC

GCTACTCGTT

GACGAATCCC

AGAATGGATA

TATTGTCGGG

AGATACATCG

TATTGATACG

AAGTAGCCGC

TI'TATTAATT

TAGTGCCTTA

TATTAAAGGA

GCTAATCGAA

ITTTTCCAAGC

AATATCNT

GGGGCAACTA

CGCACAACAA

TCGTTACTC

AAACCTGATG

AGTGTTCAAG

AAAAACACAT

CACTCTATGG

TACCTAAAA

AGTCAACAGC

TATTGGATT

GGTGGTCAAT

GCAGTCAACG

GAAAAGCAC

TTICTATGGC

CCTTTFIAG

ATTT'ITCIT

CGAAGGATGA

CACCAACGCT

ATCGC'ITGGA

GGACCGTGGT

TTCTAT1rGA

TACTCTATT

GTGGTlGITAG

TTGAAAAATT

AAACCATCGA

ATCAAGAGAT

TAAGTCCAAC

CCTTGCTAAG

AACATAGTCA

CGAGTAAAAA

TTCATCCCAG

GCTAATGGGT

CAACCAAGCC

TTI=ICAT

GAAACAAGGT

TAAAGAGCGA

AGATAATAAA

ACAAAAAAAT

TGGITACGT

CGTCAGCATT

AACCGAACTA

GATTAATGTT

AGAT1'TAAGT

ACAGCAGGA

ATTACATGA.A

ATAG

a a EF050-2 (SEQ ID NO:18 6 MNMPKN IXYFSLLMGL VLLLSACQIG A'ITKDDNQAA TKEATVELNR MrTTTLFFHG YAGTXNSFGS LLHRLEKQGA ALSGKATNPS VQVLFEDNKN NEWNQTEWIK NTLLYLQK1NY LGTYGQDTSL PKIEKFVSIG APFNDFIDTS QQQTIETELE PEKLPILLIG GQLSPTDLSD GTVPLSSALA VNA.LLRQRGT PEVDQLLIEF LWPSKK

TTQELVLLIVK

QvNKANIVGH

NGPTEKSSRY

QVTSQI IKGE

PDGTVVKERG

SMGGVSGLRY

LDYQEMINVV

NAQHSQLHEN

EFOSO-3 (SEQ ID NO:18 7 128 TABLE 1. Nucleotide and Anlino Acid Seqeuences of E.f[aecalis Genes.

TTGCCAA.ATT

GCCACAAA.AG

GGTTACGCAG

GCCACAACTC

GGAGCTTTAA

AACAATGAAT

TATCAAGTGA

TATI'TAGGAA

CGAGCACCTT

GAAAACGGCC

GTTCCAGAAA

GATGGAACGG

ACTCAAGTCA

AATCCTGAAG

GGGGCAACTA

AAGCAACTGT

GAACTAAAAA

AAGAATTAGT

GTGGCAAAGC

GGAATCAAkAC

ACAAAGCCAA

CCTATGGGCA

TCAATGATTT

CCACAGAAAA

AACTGCCCAT

TGCCGTI'ATC

CTACCCAGAT

TAGATCAATT

CGAAGGATGA

TGAGTTAAAC

TTCGTTTGGC

GCTACTCGTT

GACGAATCCC

AGAATGGATA

TATTrGTCGGG

AGATACATCG

TATTGATACG

AAGTAGCCGC

TTATTAATT

TAGTGCCTTA

TATTAAAGGA

GCTAATCGAA

CAACCAAGCC

CGCACAACAA

TCGTTACTGC

AAACCTGATG

AGTGTTCAAG

AAAAACACAT

CACTCTATGG

TTACCTAAAA

AGTCAACAGC

TATTTGGA1T

GGTGGTCAAT

GCAGTCAACG

GAAAATGCAC

=TCTATGC

CACCAACGCT ?rTTTTTTCAT ATCGCTTGGA GAAACAAGGT CGACCGTGGT TAAAGAGCGA TTCTATTTGA AGATAATAAA TACTCTAFI'T ACAAAAAAAT GTGGTGTTAG TGGTTTACGT TTGAAAAATT CGTCAGCATT AAACCATCGA AACGGAACTA ATCAAGAGAT GATTIAATGTT TA-AGTCCAAC AGATTTAAGT CCTTGCTAAG ACAGCGAGGA AACATAGTCA ATTACATGAA CGAGTAAAAA ATAG EF050-4 (SEQ ID NO:188) CQIG A ETKDDNQAA TKE-ATVELNR TTTPTLFFHG ALSGKATNPS VQVLFEDNKN LGTYGQDTSL PKIEKFVSIG PEKLPILLIG GQLSPTDLSD PEVDQLLIEF LWPSKK

YAGTKNSFGS

NEWNQTEWIK

APFNDFIDTS

GTVPLSSALA

LLHRLEKQGA

NTLLYLQKNY

QQQTIETELE

VNALLRQRGT

TTQ ELVLLNK QVNKAN7VGH NG P TKS SRY QVTSQI IKGE

PDGTVVKERG

SMGVSGLRY

LDYQEMINVV

NAQHSQLHEN

EF051-1 (SEQ ID NO:189)

TA-AAAGAAAA

TTCAGTTTAG

ACAAAAAAAG

AAAATTATTC

AGTrTTGGAA

GCI'CGACAA

OTTCCTTTAT

CATGATT=~

CCAGCTGGTC

AAACACGCAA

GCAGAAGCTG

GCGGCCATGC

GCCTCTAAGA

AGAAAATATT

GAGGCGTTCA

TTGCCCTAAT

AAGAAATAAC

CAGCCTTTGA

AATTACAGAT

AACAAATGAA

TGGAAAACCA

CTGATITAA

AATATGCCGA

GCITrGGCAC

CCTTAGTTA

CTGAAGCT

AATGTCTAAA

TGCTGCATGT

CC=~CGGCA

AAAAGAGCAT

GCAA.ATTGA.A

TGCA'ITGGTT

GCTCGTTCTT

AAAAGCCCAA

AGAAGGCTTA

GAATGTAACA

TCCACAGAT

TI'TGAAAAAG

CAAAAAAAGG CTGTGrrCCT GCTTAGTTTA ACAAATCAGC CGCAAAAAGA AACAGTTTCA GCAGCTAGCT TAGAATCAGT CATGGAGAAG CCAGATATTC AGGTAACTGG AACCTATGAT AAAGCCCTAA AAGCCGATGT ATMW1TCTCA GCAGAAAAAC TAATTAATAA AAAAAGTGTC ATTGTGCCTA ACCAAGATCA AGCAAAGTGG ATOATAGCAA TTGGTGATCC TGCAAGTGTT AAAGCTI'TAG GCGCTTGGTC TTATGTAGAA GAAGTCC ITG AATGGGTAGC TAATGCAAGT GCAGCAACCA ATTCAAAAGT AGCGATTGTT CCAATTATCT ATCCAGTTGG TAAAGTTGCC TT'IMAAAT TTTTACAGAG TCAACAATGC TTAACAAAGT AG AACAAAAATC AGCAGATGCT TTGANAATAT TGGCTTTA-AG EF051-2 (SEQ ID NO:190) MSKQ KKAVFLLSLF SLVALIAACT NQPQKETVST KKEEITLAAA ASLESVMEKK IIPAFEKEHP DIQVTGTYDS SGKLQMQIEK GLKADVFFSA STKQMNALVA EKLINKKSVV PLLENQLVLI VPNQDQAKWH DFSDLKYAQM IAIGDPASVP AGQYAEEGLK ALGAWSYVEK HAS FGTNVTE VLEWVAJA-SA E-AGLVYATDA ATNSKVA IVA AMP EAVLKKP I IYPVGKVAA SKKQKSADAF LNFLQSQQCR KYFXNIGFKL TK EF051-3 (SEQ ID NO:191) ATMT ACAA.ATCAGC CGCAAAAAGA AACAGTTTCA ACAAAAAAAG AAGAAATAAC CCTTGCGGCA GCAGCTAGCT TAGAATCAGT CATGGAGAAG TABLE 1. Nucleofide and Amino Acid Seqeuences of E. faecalis Genes.

A.AAATTATTC

AGTTCTGGAA

GCTTCGACAA

GTTCCTTTAT

CATGATTTTT

CCAGCTGGTC

AAACACGCAA

CCAGAAGCTG

GCGCCATC

GCCTCTAAGA

AGAAAATA IT CAGCCT 1TGA

AATTACAGAT

AACAAATGAA

TGGAAAACCA

CTGATTTAAA

AATATGCCGA

GCTTTGGCAC

GCTTAG=IA

CTGAACCTGT

AACAAAAATC

TTGANAATAT

AAAAGAGCAT

GCAAAT'TGAA

TGCATTGGTT

GCTCGTTCTT

A-AAAGCCCAA

AGAAGGCTTA

GAATGTAACA

TGCGACAGAT

TTTGAAAAAG

AGCAGATGCT

TGGCTTTAAG

CCACATATTC

AAAGGCCTAA

GCAGAAAAAC

ATTGTGCCTA

ATGATAGCAA

AAA~cCTTAG

GAAGTCC=T

GCAGCAACCA

CCALATTATCT

TTTTTAAAr

TTAACAAAGT

AGGTAACTGG

AAGCCGATGT

TAATTAATAA

ACCAACATCA

TTGGTGATCC

GCGCTTGGTC

AATGGGTAGC

ATTCAAAAGT

ATCCAGTTGG

TTTTACAGAG

AG

AACCTATGAT

ATTTTTCTCA

AAAAAGTGTC

AGCAAAGTG

TGCAAGTGTT

TTATrGTAGAA

TAATGCAAGT

AGCGATTGTT

TAAACTTICC

TCA.ACAATGC

EF051-4 (SEQ ID NO:192) CT NQPQKETVST K.KEEITL.AAA ASLESVMEKK IIPAFEKEHP DIQVTGTYDS SGKLQMQIEK GLKADVFFSA STKQMNALVA EKLINKKSVV PLLENQLVL I VPNQDQAI(WH DFSDLKKAQM IA IGDPASVP AGQYAE-EGLK ALGAWSYVEK HASFGTNVTE VLE-WVANASA EAGLVYATDA ATNSKVA IVA AMPEAVLKKP I IYPVGXVAA SKKQKSADAF LNFLQSQQCR KYFXNIGFK(L TK EF052-1 (SEQ ID NO:2.93)

TAAAGTAGGA

CCCACAATGG

TTACTTAGTT

NGAAATCTTC

GCAGAAAATA

TCAAAAAAAA

GTTCGTAGCA

CTAAGCTGGT

GAAGCGCAAG

GTACCATGGG

CGTCGTGGAG

NGTTTITATT

GGGAGACCAC

ATCCAGT'GT

GAACGCAAAT

T1'GGCATATT

CGAAAAAAGT

TAGCATTATC

AGAAGTGGAA

ATTGTTGGTT

AGAAGTCGGA

GAATGTA'G

AAAAAGATA

G'TTTTTAATA

GAATCAATCG

TTTG.ACAGTA

AAACGAAGGG

CTA~rAMT, ATCGGGTTrA CCC CAAACAA

CCTAAAACTG

AGTAGT'I=

GCAGCGTATC

GCACTTGCTG

GAAGTGACAT

ATATTCCTCA

CAAAAACTTC

CCATTCAATC

GTGACAATCG

GGCTGTWCT

AAGTAGTGAT

GATTCATTGC

AATGAGANGA

AACAACTTAT

AGACATACCA

GCTATCAATC

AATAACTTGG

A 1TTAGACAA TTATGTAGAA AAGGAGAATA A EF052-2 (SEQ ID NO:194)

MRXX

NLXFLLLLVL LIYIPQTTYA ENRETTEVGI GFTKTSDIPS KKNPVVNVLP QTT-IQSLSIV RSRTQ IKRLP KTGDNRITWL SWFGILFLIS SFWLFLFRQL CRKGE EFQS2-3 (SEQ ID NO:195) AGAAA.ATA GGGAGACCAC AGAAGTCGGA ATCCGGGMTA CAAAAACT'C ACACATACCA TCAAAAAAAA ATCCAGTTGT GAATGTATTG CCGCAAACAA CCATTCAATC GCTATCAATC GTCGTAGCA GAACGcAAAT AAAAGAT EFOS2-4 (SEQ ID NO:196) ENRETTEVG I GFTKTSDI PS KI(NP'JVNVLP QI'IQSLS IV

RSRTQIKR

EF053-1 (SEQ ID NO:197) TAGTCATGGC ACCATA-ACAA GGAGGAGAGA AGTGACATGA AAAAATACCT TTTGCTTAGT 130 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

TGT' 7ITAG

GAAAATAGTIG

ACAGCGACTA

TCATCCGTCC

ACCACCAATC

TTTATTAAAA

ATTAG

GTCTTTCAG

GTGTTGTCTC

GTAATTCTTC

T1'TCTTCTGG

AAGCACCATT

GGAGAAGAGA

CTTCTGTCAT

CTTTTATGGA

CACAACCACC

CGTATATGGA

TATTTATITG

AGATGAAAAA

TCAGACACTG

ACGTATGAAT

GAACCCACCA

TCCCGACAAG

GGAATCAGCC

AACAGTATTA

CGTTT-GAGA

ATCCCACAGA

AGCCACCA

GAAGATTACC

TTATCACTAT

GCAGTAGTAG

AGCAGC TAT

AGAGTCGACA

CGGAGGCGCT

AGCGACAGGT

AGGCATATTA

GGATTGTAGG

EF053-2 (SEQ ID NO:198) MKKYLLLSC FLJGLFSFCHS DTAFGEAAYE NSGVVSFYGT YEYPTEESTT ATSNSSTTTE PTXPADGGAS SVLSSGVYGS RQG3RLPATGT TNQAPFIYLG IKRRREDEKN SISSSRDCRI

ISLITIGILF

EF053-3 (SEQ ID NO:199) a a.

a a a a a a a a.

TTTGGAGA AGCAGCTTAT GAAAATAGTG GTGTrGTCTC CTTTTATGGA ACAGCGACTA CTAATTCTTC CACAACGACC TCATCCGTCC TTTCTTCTGG CGTATATGGA ACGTATGAAT ATCCCACAGA AGAGTCGACA GAACCCACCA AGCCAGCTGA CGGAGGCGCT TCGCGACAAG GAAGA EF053-4 (SEQ ID NO:200) FGEAAYE NSGWVSFYGT YEYPTEESTT ATSNS STTE PTKPADCGAS SVLSSGVYGS RQGR EFOS4-1 (SEQ ID NO:201)

TAAATAAAA

AGTGCAGTAC

ACAGATCCAG

ACTGAGCCGG

AAGCCAACCG

ATTGATCCTG

CCAACCGAGC

GAACCAACTC

GAACACCCCA

AATCAATCGG

ACACATGTAC

GTAGATAAAG

TATAAGGTTT

TTGCCACATA

TTAA!1'CAG

ATTA=IGGA

TAGTATTCGG

CAACTCCACC

TGGATCCTGC

AGCCTACAAC

GAACCCCGT

CTACAACACC

AACCAGTGAT

CTAAACCAAT

CAGGAACACA

CAAGTGAAAC

CTGTTCCGTI'

TACCTAGCGG

CAGGAGAGAA

GATTCTTTT

GGAAATTACA

TGGCGGAAGT

AAI'ACCGAA

AGAACCGCCA

ACCGAGTGAG

TGAACCCACT

AAGCGAACCA

TCCAGAAAAA

AGACGTAGTC

ACCAAGTAT

TACTCCAATT

AACCAAAACA

AAACGTTGAG

ATTCACACTC

CTTTAAAAAG

ATGAAAAAAA

ATAACAG CAT

CCAACTGATT

GTAATACCAA

CCAGAAAAGC

GAACCAAGCG

GAAAAACCTG

CCAGCAGAAC

GTTACACCTA

CCTATTGAAA

ACAACAGAAG

CCAGAAGGAT

GTAAALAGCAA

CTT17CTCTG

AATAAG.AMA

TTATTIATC

TCGCTGACGA

CTAGTGAACC

CTGATCCAAC

CAACAGAACC

AGCCAACAGA

TTACTCCAGA

CAGAAACACC

GTCGAGAAAT

CAAGCAACTT

CTGGGGAAGA

TA.AAACCAAT

GTGATGGAAA

TATTGGGAAG

AAGCTTAA

AAGC~TT

TTTAGGACCA

TACCAATCCT

AGAACCAAC

AACAACGCCA

ACCTAGTCAA

ACAACCGAAA

AAAAACTCCT

TGATAAAACG

AGCGGAGGTA

AATTGTAGCA

TAGCAGCTCG

AATGA.AAGTA

CTTCTTTGTA

EF054-2 (SEQ ID NO:202) M KKIILSSLFS AVLVFGGCSI TAFADDLGPT DPATPPITEP TDSSEPTNPT EPVDPAEPPV IPTDPTEPSK PTEPTTPSEP EKPTEPTTPI DPGTPVEPTE PSEPTEPSQP TEPTTPSEPE: KPVTPEQPKE PTQPVIPEKP AEPETPKTPE QPTKPIDVVV TPSGEIDKTN QSAGTQPSIP IETSNLAEVT HVPSETTPIT TEACEEIVAV DKGVPLTKTP EGLKPISSSY KVLPSGNVEV KASDGKM<VL PHTGEKFTLL FSVLGSFFVL ISGFFFFKKN KKKA EF054-3 (SEQ ID NO:203) 131 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

ACAGATCCAG

ACTCAGCCGG

AAGCCAACCC

AT IGATCCTG

CCAACCGAGC

GAACCAACTC

GAACAGCCCA

AATCAATCGG

ACACATGTAC

GTAGATAAAG

TATAAGG=T

CAACTCCACC

TGGATCCTGC

AGCCTACA.AC

GAACGCCGGT

CTACAACACC

AACCAGTGAT

CTAAACCAAT

CAGGAACACA

CAAGTGAAAC

GTGTTCCCTT

TACCTAGCGG

AATI'ACCCAA

AGAACCCCCA

ACCGAGTGAG

TGAACCCACT

AAGCGAACCA

TCCAGAAAAA

AGACGTAGTC

ACCAAGTATT

TACTCCAAT

AACCAAAACA

AAACCTI'GAG

CCAACTCATT

GTAATACCAA

CCAGAAAAGC

GAACCAAGCC

GAAAAACCTG

CCAGCAGAAC

G'ITACACCTA

CCTATTGAAA

ACAACAGA-AG

CCkAAAGGAT

GTAA-AAGCAA

C TAGTGAACC

CTGATCCA.AC

CAACAGAACC

AGCCA.ACACA

'rTACTCCAGA

CAGAA.ACACC

G=AGAAAT

CAACCAACTT

CTGGGGAAGA

TAAAACCAAT

GTCATGGAAA

TACGAATCCT

AGAACCA-AGC

AACAACGCCA

ACCTAGTCAA

ACAACCGAAA

AAAAACTCCT

TGATAAAkACG

AGCGGAGOTA

AATTGTAC A

TAGCAGCTCG

AATGAAACTA

9 9 9 999 9 9 9 EF054-4 (SEQ ID NO:204) DDLdGPT DPATPPITEP TDSSEPTNPT EPVDPAEPPV IPTDPTEPSK PTEPITPSEP EKPTEP'-PI DPGTPVEPTE PSEPTEPSQP TEPTTPSEPE KPVTPEQPKE PTQPVIPEKP AEPETPKTPE QPTKPWDVVV TPSGEIDKTN QSAGTQPSIP IETSNL.AEVT HVPSETTPIT TEAGEEIVAV DKGVPLTKTP EGLKPISSSY KVLPSGNVEV KASOGKMKV EF055-1 (SEQ ID NQ:205) 9 *99*

TAACAAAAGG

GGAGGTIT

AGTTTTTTA

TACCAAAATC

GCTGATCCCA

CAGACCACCA

AGCATI'CTCN

CATGAAGCAA

TTGTTITCTC

CAATG-AAAAA

TAAATGTTGA

CGAGAACACC

AGGAACCAC

CAACTGGCTC

GN LTCGCCTT

ACTAA

TTTCTTGTGT

AAAGCGTTAT

AGCGTCTGAT

CGCTCCTAAA

TGGTCCTCCG

AACGCTCCCT

AATCGG'TrG

AAAAGGGCAA

TTAATGATTG

GGTGGTTCTA

GATCCCCCAC

CAAGGAGATC

CGTACAGGGA

GCGGGAATCG

GAAAGGCTAG

TGTGTCTACT

GTTCGGTGGG

CGAAAACAGA

AACGAAGTGG

GCAAGAGTCA

TACATAGAAA

CGAGTTIAAAA

ATCTTCTCCT

GATTGAATT

T'GCGCCAGCT

TCGTTCGACA

GGCAALATTTG

GAAGGGACGA

EF055-2 (SEQ ID NO:206) MKKKRYL MIVCLLSSPS FFINVEASDG GSSSVGIEFY QNPRTPAPKD PPPKTDAPAA DPKEPAGPPQ GDQRSGGSTQ TTTGSTLPR TGSKSQANLS ILXFALIGLA GIVHRKXGRH EAN EFOSS-3 (SEQ ID NO:207)

AGCGTCTGAT

TACCAAAATC

GCTGATCCCA

CACACCACCA

GGTG.GTTCTA GTTCGGTGGG GATrGAArTT CGAGAACACC CGCTCCTAAA GATCCCCCAC CGAAAACAGA TGCGCCAGCT AGGAACCAGC TOGTCCTCCG CAAGGAGATC AACGAAGTGG TGGTTCGACA CAACTGGCTC AACG EF055-4 (SEQ ID NO:208) SDC GSSSVGIEFY QNPRTPAPKD PPPKTDAPAA DPKEPAGPPQ GDQRSGGSTQ TT'TTGST 132 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecaliks Genes.

EF056-1 (SEQ ID NO:209)

TAAATGAAAA

ATAAATGTTG

CCGGCAACAC

AAGGAACCAG

ACAGCTGGCT

GGTCTTGTCT

AACTAA

AAAAGCGTTA

AkAGCATCTGA

CCGCTCCTAA

CTG.GTCCTCT

CGCAGCTCCC

TGATTGGTCT

TrAATAATT GCGTGTTITAC GGGTGGTTCT AGTTCGCTGG AGATGCCCCA CCGAAAACAG GCAAGGAGAT CAACGAAGTG TCGTACAGGA AGCAAGAGTC TGTCGGAATC3 GTCCAGAGAA

TATTTCCCC

GAATI'AATT

ATGAGCCAGC

GTGGTTCGAC

AGGCAAACCT

AGAAGGGACC

TAGTTTTTI'T

TTACCAAAAT

TGCGGATCCC

ACAGACCACC

CAGCATCT

ACATGP.AGCA

EF056-2 (SEQ ID NO:210) MKKKRYLIIA CLLFSPSFFI NVEASEGGSS SVGIEFYQNP ATPAPKDAPP KTDEPAADPK EPAGPLQGDQ RSGGSTQTTT ACSQLPRTG-S KSQANL.SILO LVLIGEYGMV QRKXGRHEAN EF056-3 (SEQ ID NO:211) 0 0* 0 0 000000 0 0 ATCTGA GGGTGGTTCT AGTTCGGTGG GAATTGAMT rTACCAAAAT CCGGCAACAC CCGCTCCTAA AGATGCCCCA CCGAAAACAG ATGACCCAGC AAGGAACCAG CTGGTCCTCT GCAAGGAGAT CAACGAACTG GTGGTTCGAC ACAGCTGGCT CGCAG

TGCGGATCCC

ACAGACCACC

EF056-4 (SEQ ID NO:212) SEGGSS SVGIEFYQNP ATPAPKDAPP KTDEPAADPK EPAGPLQGDQ RSGGSTQTI'T AGSQ EF057-1 (SEQ ID NO:213)

TAATGTTTAT

ATCATAAAAA

AGCGNTATGG

TACGTTTATG

CAGGGAAGAA

GAAAATCCGC

AATAAAATGA

TrGGCTGCGCC

GGTTAGTTT

CTGAAGAAAA

AGAATGAAAA

ACAATTTAGC

TGTA~rCCTT

AACGA--AGAA

AGTCAATGTT GAA.AATGGGG GGTATGTTTA GGGCTA=t~A TAATTATGAA TCAAATGGTC AGAGTCAAAT GACGTAGCGT TGCTTCMGA CAAGCAGTT GATAGGAGTT AGTITCTTGG CGAGTTATT TAA

AAGGAGGA;AT

TCATTC-GGTT

AAGCGAGCT'

ATACCCAACA

TACCTAAAAC

GGATAGTCAT

TCAGATCAAA

GCNAACAAAA

CTATG;GTACC

ATCACAAGAA

AGGCGAGTCT

TTA=TAAT

0 0* 00 EF057-2 (SEQ ID NO:214) MKI IKRFSLVCLG LLIIGLXTKS XMAEENTNYES NGQASvFYGTY VYENEKESND VAYTQQSEEQ GRNNLAASGQ AVLPKTGESE NPLYSLIGVS LLGIVIYLIN

KMKREKEFI

EF057-3 (SEQ ID NO:215) AAA TAATTATGAA TCAAATGCTrC AAGCGAGCTT CTATGGTACC TACGTTTATG AGAATGAAAA AGAGTCAAAT CACGTAGCGT ATACCCAACA ATCAGAAGCM CAGGAAGAA ACAATTTAGC TGCTTCTGGA CAACCAGTTT EF057-4 (SEQ ID NO:216) EENNYES NCQASFYGTY VYENEI(ESND VAYTQQSEEQ GRNWLAASGQ AV 133 TABLE 1. Nucleotide and Amino Acid Seqeuences ofrE.f[aecalis Genes.

EF058-1 (SEQ ID N'O:217) A.

A

TGAAGAACGT

TTGGCTATTT

TGGTACACCG

ACAACTGCAT

ATGACGGATT

AA.ATATCAAG

GAGCAACGAG

CCTGGGAAAC

CCTAAAAAkAC

GTAGTT-GCTG

GATGGTTCCT

GTGGTAGCCA

TTAAATGGCG

ATCCAAGGAG

=TATTACTG

ACGGGAGAAT

GCTCCAAATA

AACAATCAAA

ACACCAAGCT

GTAAATATTC

AATTTAGTTG

GCTTATGCGT

CAAGCAAATG

GGCACACTAA

TTTAAAAATG

GAAGTTGTGA

GCCcTGGCGc

AAAATCGATG

=IiACAACAA

TI'AGAACAAA

GTCAATGA.AC

AAACACAAAG

GGCGCAGTCT

TAA

TCTATTFIGC-T

TATCAAAAAC

TTAGTACCTT

TTGCAGAAGA

TACCAGATCC

GACTGGCAGA

CGGCAGGCGC

CTGTTGCTCA

AAAATrGTAA

CTACGAATAT

ATAAATATGG

ATGATGGTAG

CAGAATTITGT

TCAA.AGATGG

GGAAAAGTTA

TAACAGTTAA

ATGCAGAA1T

CACCTGTTGA

TAGATGGTAA

CATTGGGGAT

ATAAACATGA

TATATGATGG

GCTTCACTGT

AATTCGTTTA

AGGCGAATGT

CAGGTGGGAA

GAGCTTCCTT

AAACAACGAA

CGGCTGATGG

CTGTAGCTCC

AATCATATG

GTACCTTACC

TGCTACTrAT

TCACGATTGC

AA.AAAAGAGG

GTTACTAATT

AAATGGGGAG

GC~rATICA-A

TGTGACGTT'

AAGCGTTGAT

AGGAACCACC

AGATGCAGTG

GGTGGTGGCG

AACAGAAGAA

TTTACATGTG

TATTTCTAAA

ATTATATACA

TGAAATTGGC

AAATCTTGAG

AATTGAAAAT

AAAAACAGTC

AGATGTGGCA

TGCAGACAAA

TGCAGCCTTA

GGATACAGTG

CGCCGTTAAT

CTTTATGCAT

TGATAACGGT

ACGTTTCATT

TGTCGTCCGT

AGCAGCAACT

ATI'AGTTGAT

TGATGATTAT

CACAACAGAA

TTCAACAGGT

TGCAGrGAGTC kGGCCTGCTA

AGAGAGAAAAA

ITGCCACTrT

AGCGCACAGC

AATAGCGGCA

PGTATTTATA

GCAGCTAAAC

6-TGCAAATG-

TATACCATTA

TTCCCAGTT

TTAGCGGTTG

AAAAAAGTAG

AGCGAAGGCT

TGGACAACGG

GAAA.ATCATT

GTTGGTTCGT

CAAACAAAAA

AAAAATCATA

ATTGGCGAAA

GAAGGCGACG

ACITTI'GATA

A=TCTCCTG

CCAGCGTATA

TTAAATGAAA

CATACCGACG

AAACTCGATG

GATCAAAACA

TGGGTGAAAA

ATCACAGGGC

GTCTTGTrAA

AACCTAGTTT

GGCAAAGGAA

TACTTGCTA

kTCATTGGGA rGA.AGcAArI'

TCACAAGTGT

TCGT'GATTCA

AAGAAATGAG

ACGTGACGAA

AAGCTGTCCA

GGAATGTCAC

AAGAAGAACC

ACGAAATGAT

TTCATA'ITTA

GAACTGCTGA

CAC CAGGCAC

ATAAAGAACA

TCACAGAAC

ATATITAGA

CACCATTTAC

CCTCTAAAGT

AAALTTAAATA

CTAATAAATA

ACGTCAC ITC

AAAAITATCA

TrCCTACGCT

AAGCAGATCC

ACCAAACACC

GCGATGTGCAC

GCGACACAC

CAAAAGCTGA

TTAAATACGG

CAAATCGGAT

CACCAGAAAA

TCTACGTTA

GACGTAGAAA

TGGTAGTCAT

A4.AAAAAACTT

PTTAGGGACA

CAAAAAGAAA

CGAGTTTGAT

CGAATTTTAC

AAGTTTAACT

TGTTCAGTTA

AAAAGAGGGT

CAAGCAAACA

TCCTAAAAAT

AAATG.AGGA

AGTAAAATAT

AGCAAAACGC

AGAGAATGGA

AGAAGTAAAA

AA'FrGAAGCA

TGATAAAACA

TCAAATTTCT

CGTCAAATTC

TGGAGAGTAT

AGTGACTGAA

AACACCAGGC

TACCAAAGGC

ACCAACTGTT

AGCGACACAA

AAATTATTTG

AGCAACTACT

TACCTATTAT

TGAATTTGTG

AGTACCAAAC

CTTAGGAAGT

AGAAAATGCT

EF058-2 (SEQ ID NO:218) MKQLKKVW YTVSTLLLIL PLFTSVLGTT

TAFAEENGES

QRAAGASVDA

VAATNMVVAF

NGAEFVISKS

GELTVKNLEV

PSLDGKDVAI

YALYDGDTVI

KNEANVDNGH

IDETTKAATW

NEQSYGTTEN

AQLVI HKKKM

AKQAVQSLTP

PVYEMIKQTI)

EGSPGTVKYI

GSY ILEEVKA GEK IKYQISV

APENYQVTEQ

TDDQTPPTVE

VKTKAE-ATTF

LVSPEKVPNK

TOLPDPLIQN

GKPVAQGTTD

GSYKYGTEEL

QGVKDGLYTW

PNNAELIENQ

NIPLGIADKE

ANGFTVAVNP

WVTGGKRFIK

TTTADGLVDI

HKGTLPSTGG

SGKEMSEFDK

ANGNVTVQLP

AVVI{IYPICNV

TTOKEQAKRF

TKTPFTIEAN

GDANKYVKFN

AYIPTLTPGG

VDG-DVTATQA

TGLKYGTYYL

KGIYVYLGSG

YQGLADVTFS

KKQNGKDAVY

VAND)GSLHVK

ITGKSYEIGE

NQTPVEKIVK

LVOKHDAALT

TLKEVYFMHL

LAGASFVVRD

EETVAPDDYV

AVLLLIAGVY

IYNVTNEFYE

TIKEEPKEGV

KVGTAENEGL

NDFTEAENGT

NDTSKvDKTT

FDNVTSGEYA

NEKADPTKGF

QNSDTANYLK

LLTNRIEFVV

FARREKENA

EF058-3 (SEQ ID NO:219) TABLE 1. Nucleotide and Amino Acid Seqeuences or E. foecalis Genes.

ACAAGA AAATGGGGAG AGCGCACAGC TCCTGATTCA CAAAAAGAAA

ATGACGGAT

AAATATCAAG

CAGCAACGAG

CCTGGGAA.AC

CCTAAAAAAC

GTAGTTGCTG

GATGGTTCCT

GTGGTAGCCA

TTAAATGGCG

ATCCAAGGAG

TTTATTACTG

ACGGGAGAAT

GCTCCAAATA

AACAATCAAA

ACACCAAGCT

GTAAATATTC

AATTTAGTTG

GCTTATGCGT

CAAGCAAATC

GGCACACTAA

TTT AAAAATG

GAAGTTIGTGA

GCCTTGGCGG

AAAATCGATG

'TTTACAACAA

TTAGAAGAAA

GTCAATGAAC

AAACACAAAG

TACCAGATCC

GACTGGCAGA

CGGCAGGCGC

CTGTTGCTCA

A.AAATGGTAA

CTACGAATAT

ATAAATATGG

ATGATGGTAG

CAGAATTTGT

TCAAAGATGG

GGAAAAGTTA

TAACAGTTAA

ATGCAGAATT

CACCTGTI'GA

TAGATGGTAA

CATTGGGGAT

ATAAACATGA

TATATGATGG

GCTTCACTGT

AATTCG'TTA

AGGCGAATGT

CAGGTGCGAA

GAGCTTCCTT

AAACAACGAA

CGGCTGATGG

CTGTAGCTCC

AATCATATGG

GTACCTTACC

GCTTATTCAA

TGTGACGTI

AAGCGTTrCAT

AGGAACCACC

AGATGCAGTG

GCGTGGCG

AACAGAAGAA

TTTACATGTG

TATTTCTAAA

AT1'ATATACA

TGAAATI'GGC

AAATCTTFGAG

AATTGAAAAT

AAAAACAGTC

AGATGTGGCA

TGCAGACAAA

TGCAGCCTTA

GGATACAGTG

CCCCGTTAAT

CTTTATGCAT

TGATAACGGT

ACGTTCATT

TGTCGTCCGT

AGCAGCAACT

ATI'AGTTGAT

TGATICATIAT

CACAACAGAA

AATAGCGGGA

AGTATTTATA

GCAGCTAAAC

GATGCAAATG

TATACCA1TA

TTCCCAGTTT

TTAGCGGTTG

AAAAA.AGTAG

ACGCGAAGGCT

TGCACAACGG

GAAAATGATT

GTTGGTTCGT

CAAACAAAAA

AAAAATGATA

ATI'GGCGAAA

GAAGGCGACG

AVITrTGATA AT1'GCTCCTG

CCAGCGTATA

TTAAATGAAA

CATACCGACG

AAAGTCGATG

GATCAAAACA

TGGGTGAAAA

ATCACAGGGC

GTCTTG =AA AACCTAG7I'T

AAGAAATGAG

ACGTGACGAA

AAGCTG-TCCA

GGA.ATCTCAC

AAGAAGAACC

ACGAAATGAT

TTCATATTA

GAACTGOCTGA

CACCAGGCAC

ATAAAGAACA

TCACAGAAGC

ATA7TIAGA

CACCA=IAC

CCTCTAA.AGT

AAA'FrAAATA

CTAATAALTA

ACGMtACT1'C

AAAATTATCA

TTCCTACGCT

AACCAGATCC

ACCAAACACC

GCGATGTGAC

GCGACACAGC

CAAAAGCTGA

TTAAATACGG

CAAATCGGAT

CACCAGAAA-A

CGAGTTTCAT

CGAATTI'TAC

AAGTTTAACT

TGTTCAGITA

AAAAGAGGGT

CAAGCAAACA

TCCTAAAAAT

AAATGAAGGA

AGTAAAATAT

AGCAAAACC

AGAGA.ATGGA

AGAAGTAAAA

AATTGAAGCA

TGATAAAACA

TCAAATTTCT

CGTCAAATTC

TGGAGACTAT

AGTGACTGAA

AACACCAGGC

TACGAAAGGC

ACCAACTGTT

AGCGACACAA

AA.ATTATTTG

AGCAACTACT

TACCTATTAT

TGAATTTGTG

AGTACCAAAC

a a.

a a

S

a EF058-4 (SEQ ID NO:220) EENGES AQLVIHKKKM TDLPDPLIQN SGKEMSEFDI( YQGLADVTFS IYNVTNEFYE a *a.a a. a a.

QRAAGASVDA

VAATNMVVAF

NGAEFVISKS

GELTVKNLEV

PSLDGKDVAI

YALYDGDTVI

KNEANVDNGH

IDETTKAATW

NEQSYGTTEN

AKQAVQSLTP

PVYEMIKQTD

EGSPGTVKY I

GSYILEEVKA

GEKIKYQISV

APENYQVTEQ

TDDQTPPTVE

VKTKA-AT=F

LVSPEKVPNI(

GKPVAQGTTIJ

GSYKYGTEEL

QCWKDGLY I PNNAEL IENQ

NIPLGIADKE

ANGFTVAVNP

VVTGGKRFIK

TTTADGLVDI

HKGT

ANGNVTVQLP

AVVHIYPKNV

TTDKEQAKRF

TKTP FT lEAN

GDANKYVKFN

AY IPTLTPGG

VDGDVTATQA

TGLKYGTYYL

KKQ1'NGKDAVY VANflGSLHVK

ITGKSYEIGE

NQTPVEKTVK

LVDI<HDAALT

TLKFVYFMHL

LAGASFVVRD

EETVAPDDYV

TIKEEPKEGV

KVGTAENEGL

NDFTEAENGT

NDTSKVDKTT

FDNVTSGEYA

NEKADPTKGF

QNSDTANYLK

LLTNRIEFVV

EF059-1 (SEQ ID NO:221)

TAGATTGGAA

TTAGCAGGGG

ACAACAGGGA

GAGCCAGAGC

ACCCAACCTA

CCAACAGAGC

GTACCAGAGC

GAATGAAAAT

GAAGCAGTT

GTGTTTTACC

AACCAACAGA

GTGAGCCTTC

CA.ACAACGCC

AACCAACAGA

GAAAAA.AATG

TTCTGCTTAT

AGATGAACCG

GCCAAGTACA

AAAACCGACG

AAGTAAGCCA

GCCAAGTGTA

ATrATTATTG

GCGCAAGAAT

AATGTACCAA

CCAGAGCAAC

GATCCTTCGT

GAGCAACCA

CCAGAAAAAC

CCTTATTCAG TACAAGCCTT CAGAAGGAAA TCTTGGTGAA CTGACCCA.AT AACGCCAAGT CATCCCAACC GTCAACACCA TACCAGACGA ACCGAGCGTA CAGAGCCAJ4C AACGCCAAGT CAGTAGAACC AAATAAACCA 135 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faca/is Genes.

ACCGAGCCAG

GAGCAACCAA

CAACAGCCAA

GTGACGACAC

CCTTTAACAC

AGTGGCAATG

GAAAAAATGG

TTAA=TATA

AAAAGCCTGT

CAGATGTGGT

CTGTTCCAAT

CCATTGAAAC

AAACGCCTGA

TACAAGTGAA

GCATAA'ITGG

AAAAACCTAA

GCCAGTTGTT

GGTAAAGCCA

TGAAACGAAT

AGCAAGCGGA

TCGATTAAAA

AAGTGCTGAC

GTCAATCGCT

AGTCTAG

CCrrGAAkAAAC

AATCGAGAA.A

AACCT=CAG

GAAGCAAMI

CCGATTAAkA.

GGAAAAATGA

GGTGTATGTT

CAGTTGTACC

TTGCAACAGG

AkAGTAACACA

TCGCAGTGGA

CTGAATATAA

AAGTAkCTTCC TGACTrGTTTT

ACAACAACCA

AGAATCTACA

TGTCCCAACT

TAAGGGCGT

AGTATTACCA

TTIACACTGGT

ATCAGGAATC

EF059-2 (SEQ ID NO:222) MKKMI IIALFSTSLL AGGSSVSAYA QESEGNLGET- TGSVLJPDEPN VPTDPITPSE PEQPTEPSTP EQPSEPSTPT EPSEPSKPTD PSLPDEPSVP TEP'TPSKPE QPTEPTTPSV PEQPTEPSVP EKPVEPNKPT EPE-KPVPVVP EKPVVPQQPE QPTDVVPN GEIATGESTQ QPTVPIETNN LSEVTHVPTV TTPIETASGE AIVAVDKCVP LTQTADGLKP IKSEYKVLPS GNVQVKSADG KMKUVLPYTGE KMGIIGSIAG VCLIVLSGThL IYKKRKV EF059-3 (SEQ ID NO:223) 0 0 a.

a 5* S S

S.

S S 0 50 a a 00~ a *SaSSS

S

*000 a AGAAGGAAA TCTTGGTGAA

ACAACAGGGA

GAGCCAGAGC

ACCGAACCTA

CCAACAGAGC

GTACCAGAGC

ACCGAGCCAG

GAGCAACCAA

CAACAGCCAA

GTGACGACAC

CCT'ITAACAC

AGTGGCAATG

GTC TTACC

AACCAACAGA

GTGAGCCTTC

CAACAACGCC

AACCAACAGA

AAAAGCCTGT

CAGATGTGGT

CTGTTCCAAT

CGATTGAAAC

AAACGGCTGA

TACAAGTGAA

AGATGAACCG

GCCAAGTACA

AAAACCGACG

AAGTAAGCCA

GCCAAGTGTA

GCCAGTTGTT

GGTAA.AGCCA

TGAAACGAAT

AGCAAGCGGA

TGGATTAAAA

AAGTGCTGAC

AATGTACCAA

CCAGAGCAAC

GATCCTTCGT

GAGCAACCAA

CCAGAAAAAC

CCTGAAAAAC

AATGGAGAAA

AACCTTI'CAG

GAAGCAATTG

CCGATTAAPA

GGAAAAATGA

CTGACCCAAT

CATCGGAACC

TACCAGACGA

CAGAGCCAAC

CAGTAGAACC

CAGTTGTACC

TTGCAACAGG

AAGTAACACA

TCGCAGTGGA

GTGAATATAA

"rQTAC

AACGCCAAGT

GTCAACACCA

ACCGAGCGTA

AACCCCAAGT

AAATAAACCA

ACAACAACCA

AGAATCTACA

TGTCCCAACT

TAAGGGCGTT

AGTATTACCA

EF059-4 (SEQ ID NO:224) 500 a *0 0 S a a.

EGNLGET TGSVLPDEPN VPTDPITPSE PEQPTIEPSTP EQPSEPSTPT EPSEPSKPTD PEQPTEPSVP EKPVEPNKPT EPEKPVPVVP QPTVPIETNN LSEVTHVPTV TPIETASGE GNVQVKSADG KMKV

PSLPDEPSVP

EKPVVPQQPE

AIVAVDKGVP

TEPTTPSKPE QPTEPTTPSV QPTDVVVKPN GEIATGESTQ LTQTADGLKP IKSEYKVLPS EF060-1 (SEQ ID NO:225)

TGAAAA~ATAG

ACGTTGGGAA

GCAGAAGAAG

AAAACGGAAG

TCACAACAAC

CAGACGTCAT

GCCTGTAC

AA.ATAA

ACAAGGAGCA

TACITIAAC

TAGGGCAAAC

GTGTCA'ITAA

AAGACAAAGT

TACCAAGGAC

TCGTAGTACT

CGCGATCATG

CGTTGGCATT

GAATATCGGT

GCCAATAGAG

GAGCAGAAAA

AGGTGAACGA

AACAAGTTTC

ACAATGAAAA

GCGAGTCTAA

GTAACG=C

CAACCAGTCA

ACCACTGCTA

AATAGCACGT

TATTATTTGA

GTAAAGGGTC

TTGT'TCTI'C

ATGGAGGAAA

CTGATAAAGA

AA-ACCAATCC

GGCTTTACAG

ATA-AAAAAG

ACTI'CTGGTG

TGAGAGTTTT

AGAGCCACTA

TAAAAAAACG

GACTAATGCA

CCTTGGTATT

GAAAAAGGAA

EF060-2 (SEQ ID NO:226) MMT HKSKGSLLVT LGILLTVGIA SLIVSSESFA EEVGQTNIGV TFYGGKEPLI( 136 TABLE 1. Nucleotide and Amino Acid Seqeuences; of E. foecalis Genes.

TEGVIKPIEQ PVTDKDKKTS QQQDKVSRKT TAXTNPTNAQ TSLPRTG;ERN STWLYSLCIA CLLVVLTSFY YLNKKRXKEK EF060-3 (SEQ ID NO:227) AGAAGAAG TAGGCCAAAC GAATATCGGT CTAACGTTCT ATGGAGGAA.A AGAGCCACTA AAA.ACGGAAG GTGTCATTAA GCCAATAGAG CAACCAGTCA CTGATAAAGA TAAAAAAACG TCACAACAAC AACACAAMGT GAGCAGAAAA ACCACTOCTA AAACGAATCC GACTAATGCA

CAGACGTCAT

EF060-4 (SEQ ID NO:228) EEVGQTNIGV TFYGGXEPLK TEGVIKPIEQ PVTDKDKKTS QQQDKVSRKT TAKTNPTNAQ TS EF061-1. (SEQ ID NO:229)

S.

S. S

S.

OS

5 0

S.

S.

5 5 5* 0* 5 S 0 @0 5 5 eSS 0

TAATGGAACG

ATAATGATGA

AGTGA-AArI'T

GAAGTACCAA

CCACCTGTAG

CCGACAACAC

GAGCCAAGTA

GAAAAAACTG

CCAACCAAC

GGTACACAAC

CCTAGTGTAA

GGTGTTCCAC

TTGCCTAGCG

ACCGACAGAA

AAAAAAMTCT

CTGCTTTGC

CAGAACCAAG

ACCCTGTAGA

CAACAGAACC

AACCAGTAGA

TGACACCAAC

CAATCGACGT

AGCCAACAGT

CAACACCTAT

TTACACAAAC

GAAATGTAGA

GAAGATT=IG

TTTTGCTAGT

ACA.AGAAATT

TACACCAGAA

GCCACCTATT

TACAACTCCT

ACCTGAAAAA

TAAACCAACA

TGTTGTAACG

CCCTA ETGAA

TACAACTACA

AGCAGAAGG

AGTAAAAGGT

AACTTACAAA

TTATTTAGTG

ATCCCTGATG

AAGCCAACAG

ACACCAACGG

ACAGAGCCAA

CCAGTTACAC

GAATCTGAAA

CCAACACGG

ACAAGTAAT

GACGGAGAAA

TTAAAACCTA

AAGG;ACGGTA

GCCGTAGCGG

TTAAAATTAA

CCACACTACT

ATACTACGAC

ATCCAACACC

AGCCAACAGA

GAACCAGA

CAAGCAAACC

AACCAGTACA

AATTAAATCA

TGGCAGAAAT

ACATTGTAGC

TTCAATCNAG

AAATGAAGGT

TATCTTGTCT

AATGGAGGAA

ATTGGGGGA

ACCGCCCATT

GCCAATTGAG

ACCGACAGAG

ACAACCAACG

AGCAGAACCC

ACCAGCAGAA

CGCTGGAAAT

CACGCACGTG

TGTAGAAAAA

TTACAAACTA

TTTACCATAC

GTAG

ACAGGTGAAG AAATGAATAT CTIT'IATCT EF061-2 (SEQ ID NO:230)

S

00 0

OS

OS

MMXJ I LFASL

VPTEPSTPEK

PSKPVEPEKP

TQQPTVP lET

PSGNVEVKGK

FSATLLFCGS

PTDPTPPIEP

VTPSKPAEPE

SNLAEITHVP

DGKMKVLPYT

EISAFAQEI I PVDPVEPP IT

KTVTPTKPTE

SVTTPI'rrrD

GEEMNIFLSA

PDDTTTPPIE

PTEPTEPTEP

SERPVQPAEP

GENIVAVEYXG

VAVSCL

TTPTEPTTPT

SKP IDVVVTP

VPLTQTAEGL

EPSEPEQPTE

TGELNHAGNG

KPIQSSYKVL

EF061-3 (SEQ ID NO:231) GAAA=I CTGCTI'TGC ACAAGAAAIT ATCCCTGATG ATACTACGAC ACCGCCCATT

GAAGTACCAA

CCACCTGTAG

CCGACAACAC

GAGCCAAGTA

GAAAAAACTG

CCAAGCAAGC

GOTACACAAC

CCTAGTGTAA

GGTG'rTCCAC

TTGCCTAGCG

CAGAACCAAG

ACCCTGTAGA

CAACAGAACC

AACCAGTAGA

TCACACCAAC

CAATCGACGT

AGCCAACAGT

CAACACCTAT

TTACACAAAC

GAAATGTAGA

TACACCAGAA

GCCACCTATT

TACAACTCCT

ACCTGAAAAA

TAAACCAACA

TGTTGTAACG

CCCTATTGAA

TACAACTACA

AGCAGAAGG

AGTAAAAGGT

AAGCCAACAG

ACACCAACCC

ACAGAGCCAA

CCAGTTACAC

GAATCTGAAA

CCAACAGGGG

ACAAGTAATT

GACGGAGAAA

TTAAAACCTA

AAGGACGGTA

ATCCAACACC GCCAATTGAG AGCCAACAGA ACCGACAGAG GTG-AACCAGA ACAACCAACG CAAGCAAACC AGCAGAACCC AACCAGTACA ACCAGCAGAA AATAAATCA CGCTGGAAAT TGGCAGAAAT CACGCACGTG ACATTGTAGC TGTAGAAAAA ITCAATCINAG TTACAAAGTA AAAT'GAAGGT TT 137 TABLE 1. Nucleotide and Amino Acid Seqeuerices of E. faccalis Gencs.

EF061-4 (SEQ ID NO:2]2) QEII PDDTTTPPIE VPTEPSTPE( PTDPTPP IEP PSKPVEPEKP VTPSKPAEPE TQQPTJP ET SNLAEITHVP PSGNVEVYKGK DGKMKV PVDPVEPPIT PTEPTEPTEP TTPT-EPTTPT EPSEPEQPTE KTVTPTKPTE SEXPVQPAEP SIXPIDVVVTP TGELNHAGNG SVTTPITTD GENIVAVEKG VLTQTAZGL KPIQSSYKVL EF062-1 (SEQ ID NO:2]3) o

TGATTCTTGA

CACTGGGTAA

GATAATGTAC

AACCCCGACC

GTACAAAAAG

GGTACTGAAC

GGAGCTGAAA

CTAGGGCAGC

CCTA-AAGAAT

;AAXTACCG

GCTGCAAAAG

CAAGCAGCTG

GCAA.AAA6ACA

AAGAACGAAA

GTCGTG.ACGA

GATTTTAATA

ATGGNGGGA

TCTAATGGTG

GGACTAAACG

CAATCCTCAC

ACNGCTTTTA

AAGT-TC L=G

GCGCTGTCTT

GATTTTGGGG

GATGGAAAAT

AATAGTGATT

GCNAATGGNC

GTATCTAGTC

ATTCAATT

AAAGCCAATG

NTCAkATGATT

ACAGAACTTG

GATGCAGAAA

CTNAACGANA

GTGACTGCTT

AACGCNGACG

GTACTGAAAA

GAAACGGTAA

AAAGCAGATA

CAAACAAAAA

ACNGAAGAAT

TGGCACGCTA

GATAT1'TCTC

AATCAAGCAT

AGCAACAAkAT

CTGTCCCTAT

AAGCCGCGGA

TGCAGTCAGA

ACACTAC LTC

AAAGTTCAGC

AATCAGCACA

CAACAGAAGT

TTGA-AACACC

TTwGTAAAA

AAAAAGAAGT

AATTAAAAGC

AAGCNGAAAA

ACAGCTATGT

AAGACACTAA

AAGTAAATGC

AAGNTACTIGG

GGTATGCGGT

CTAGrTTT

CAAGCCAAAG

TTGGTACAAA

ATCGTCAGG

CTTTAAATTC

CNAACAATGC

TTIACTCACC

GGC-ACGCTGT

GTATTI'CCTT

CGCAATGGTT

ATGGGAATCC

TCGTTCCTGT

TAAATGTGAA

CCAA.AGTAGA

A.AGTGACGAT

AAGACTNAA.A

CITATGANCT

GNTCNGTTGT

ATGTAGAAGG

CAAATACAGT

AAAACGGTAC

TTTATTATGA

GGGGCATCAA

rrACNAANTA

CCTACATTCT

TATTGGCNGC

TCTTTTTCTA

ATTAGATACG

AAAGGAAACA

TCAACCGACC

TACCCCAAAT

AGAACAACCA

TCCACCAGCT

AGACGTTGAT

ACCAGCAGAA

AGACCAACTA

CAAAAATGAA

AGAGCGNTAN

CAATGAAGCG

AATTTCGTCG

AGGGGATTCA

CAACTI'CCAG

TC TITAGAA

AGGACGTAAA

TGGAACGTTA

CAGAGTCAAT

TAAAGAAGTA

AAGTI'AACG

GTTCAAATAC

GGAAGATATI'

AGGTCACAAG

TTCTTTTGGT

TGCCTTTAGN

AAAAGAACCA

NCTTGTGCCN

NCGTGGCGAT

TCCPIAAAACA

TGATTTATCC

AGCTGTTGCT

CAATTTAGAT

1 TAGCAATC

CGATTTTGAA

GATTAACCAT

AGTTGGCACT

AGTGAAATCT

TGATGTCTTG

TGACCTTAAA

TTI'AGAAAAC

NTTAAATGAA

AGTC.TGTTAG

CAACCAGAA.A

CCTAAAACGG

AAAGTAGAAG

GATACCACAA

GTAGTAAGCC

GAAAATGAAG

AAAGCAGTrG

GACTTAGGCA

CAAAAAGAAC

AAAATTGCCA

GANAAAGAAG

ATTAGTAAAA

ATTAAAGGCG

AAAGATATCT

AATTCCTTTG

AAAAATAAAC

ATTACAAAAG

AATGCAGTAT

GGTAAGGATG

CTACCAGATA

AATAAAGGTG

ATTAATGGNT

CACTATGGCA

AATGCCTACT

ATGACAACAA

ACTAACTTAA

GAAAAAGCAA

AATAAAGAAG

TCTTTACAAT

GTAACNAAAC

AAAGTGAAAG

GCAGC14ATTA

CAAAACACCC

GGGTATAAAT

AATACAGCTG

GTGCCAGGTA

GTTTCTCTAC

TCCOAACGTC

GACACGACCC

GTAGGGGANA

AAAGACAATA

GGAAGCAATA

GAAAGCAAAA AAACAATATA AGACATATAA

GAGCCGTAGG

CAACGACGGT

CAGTMDTCTGA

AAG-TAGCGCC

ACGCGCAACA

CTGAAACAAC

TGAATAA.ATC

ATGAAGTA.AA

ACG=IC'C

AAGCGAAAAA

A6AGAAAATGC

TCGCNGAATA

ACCTAGTGTT

GAAAATTTAT

TTACAAAATT

TAAAAGAGGC

CAGTIACAGT

CAGAATTTGT

TTTCAAACGA

TTAAAACACG

AAGATACTCC

GCCATGCGGA

CNTATGTGAA

CAGGACCTTC

TTGGTTCAGG

AAGGAAAAAG

ATGCCCAATC

CGATIGAATI'

TCACTGATGG

ACA'IGTGAC

AAGGGATICG

IIATCAAGC

ATTCAGGAAN

TCACAGCA6AT

ATTTACTTGT

TTCAGCTGAC

GTAATCCTTC

ATGATAAAGA

CAGCNAACTA

ATGATCG=

AAAC=AAA

AAGAC'ITGAC

AAGTAGGCAA

AGCTAAGA6AT

P.TTAGCTACT

TCAACCCAAT

AGAAGCAACA

PGAAAATA.AA

ACCAACAGTA

CAATGAACCT

A.ACGTCCATT

AAAAGATCCA

TAAAGATITA

GATTGCCCAA

AGAAATTGCG

CAACAAGCAT

CGATCAATCT

CAAAGCAACT

ACGGAAAGAT

AAATCTTGGG

GACCTATACA

TTATGA.ACTA

TCCGATTATC

CTTAACGAT

ATTTGCGTAT

ATTTGTTTCT

AAAACAAGCG

TGGATTGAAA

TGTAGGTCTA

TAATGTGCCT

AGTGAAGCCT

CAATNGATAC

NCAGAAAAAT

AGGGGATACG

AGATACNT 7 AGACGCAkAGT

AGCTAAAGAC

GATGAAAACC

CTTGCCGTTT

AAANGATGGN

cAAAGATGTA

TATTCCGTTA

TGGcGGAATN CACAGrGNAA6A AGcAGGAACA

GTT'TACNATG

ACAAGc [TG 138 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

TCTGTGTATC

AAACCAACCA

CGTGGTGATG

GCCTTI'GATA

AANGTGACAC

AACCAGTTCA

CCACAAGCCT

GTCAAAGCCG

CGAATTAAAA

GTTATTAAAG

TTCTTCTATG

TGGTCGATTA

TTTGCCAATT

TCGA.AACTAT

rI'TrTNGATG

ATTGGTGTAG

AATGAGAAGA

CCAGAAAAAA

'ITAGTGGTAG

GTCTTATTAA

AAACGTAGAA

TGGAAGTCGA

AAGAC1TTGT

AAGCCGTTCA

TTCT'PrCTTA

CAGTCGATCT

CAATCAA.AGA

CGATCTfCNTG TTAT'rCTAGC

ATGT'ITCTGG

CCAATACNGT

TNCGTGACAA

AATTTACAAG

GCGATAAACT

CTAA'I=T

TCACGATGAC

CGATGAATCT

AACGAATTGC

TTAAAACNAA

CAGTGATTrGT

AAAAGGCAAG

CGGTAGCTGG

AAGAAACAAA

ACGGATNAAA

NCGTITCTAAT

TAACAAGAAA

TGAAATGACN

TGCGACACGC

CTIV4CTTCGT

GGACCATGCC

GNATCTGG

NGATGTTTAT

TGTCAACCAT

ACAAAGTCA

TACTGACAT

AGACGTCAAA

TTAGCAGAC

CTTTGAACAA

AAAAGAAAAC

GGCAGGAGAC

TACGGTAGTG

ACCACCAACA

TGTNGTGCCA

TAGTTTAGCC

ATAA

ACAGGTGACG

ACN4GTGGTCA

GGGGAAGANA

T5GGACTTAA

GTTCTTTCT

GTCAALAGATT

AAAGGCACGG

CAAGAATTGC

AATTCAGCGG

ATCCAAAAG

AATGGNGCCA

6CCTGCAGAAT

CATGACAAAT

GGAACCAAAG

=CGTAGTGA

AAAAACGTTG

GT ETACAACA

ACNCATACGC

CCAAAA-ACAC

GAATTGCCGC

GCAATCCTTG

TAGAAAACAC

CCCATACNCC

?I'AANCATGG

AAGGGTACGA

TCGATGATTA

CTAAAGGGGN

TGACNATNTC

GTGTAACNCT

AACAAAATAC

TGAANCCTAA

CAATCAAATT

ACCCTCGNCT

'FTAGTGGCCA

TIGAATAAAGG

AA.ATCACGGC

CACACTCATG

CAATCGAAGA

CAGAAkAAACC

CGCAAGCACC

AAACAGGCGA

GCTTAGCAGG

GCAAACAGAA

TGATGATCCA

AAAAGTNGCT

TAAAGACTTT

CCATGAAACG

AGACATTACC

TGCCAAAGAC

CCCTACAAAA

ATTTGGNCAA

AAAAGACGTG

AGGGGAGAAN

TGTGGAAGAA

ATGGTCTGTG

GGACGACATT

CAGTCAAGCC

GAAAGCGTTC

ATCTTTCAAC

ACAAACNCCA

AGTAGAGCCA

AAAACAAAAT

CTTAGGCTTT

C

EF062-2 (SEQ ID NO:234) MKAKK QYKTYKAKNH WVTVPILFLS VLGAVGLATD NVQAAELDTQ PETTTVQPNN

PDLQSEKETP

AEKSAQEQPV

ITVVEKPAED

KNKAEKERXX

FNKVNAGDSK

LNASYLGRKI

FFDASGKEVL

GKFYSPEDID

SSAQWFAFXT

NDLNVXRGDS

NXKDXKAVAA

VKNVEGDFEN

TKIYYEVKSS

ISJAYLLENK

YNKELVRSNT

FDTVDLATGV

QAFILAXGGQ

IKVGDKQSQN

ANSNFVLADC

GVERIAAGDV

VVEKASVVPE

KTAVSEEATV

VSPETTNEPL

LGNVSSKDLA

KEVAEYNKHK

DIFTKLRKDM

TKAEF'VYELQ

PDKDS PFAYA

YGTGPSGLKN

NLNAQSVKP I

LQYIVTCDTT

AINSGXAKDV

TAVQLTXDGE

ERPANYGGXT

DNKDLTFTMN

VVTHTPDDPK

SFFDDYDETX

ELRVTLPTKV

GATIKLGEXF

TKVNKGDDI S

YNTIEESFNN

LPQTGEKQNV

QKDTTSQPTK

GQPTE.VAPAE

AKEKEVDQLQ

NENSYVNE-Al

GGKXTGNFQN

SSPSQSGTLN

LSSLNSSLTN

SDWDAVGHKN

FNYGNPKEPE

ELAKVDPKTV

TASYXLNLDQ

TVTN'IVINI{V

EEWGMNDVLD

QALLAALNEG

PTKAVHNKKG

VTP IKDLLRV

KADVSGDVYN

FYEFTSSDI P

KLFTMTFEQG

EKIKTNTVVT

LLTVAGSLAA

VEEVAPENKC

NEVNKSTSIP

KEQAKIKIAQQ

SKNLVFDQSV

SFVXE-ANLGS

AVFSN.DP IIT KGG14AEFVSD

AYFGSGVGLA

KATIEFNXYK

TKQGIRDTFD

NTVTAMMKflTN

PGSNPSKDVK

TTHDRFTGKW

SNKVGKQAWS

EXIXHGKVAR

YDSKGXDITN

SAEQNTFGQR

AEYAGVVEEW

VVKITASQAF

HTPEKPQTPP

MLGLAGLGFK

TEQSSATPND

KEFETPDVDK

AAELKAKNEK

VTKDTKIS SI

NGGYAVLLEK

AF IGTNRVNG

FGANNAFKYI

NGRI SFSFGM

ANVVPVLVPN

AEKVTIDLSK

ADGSVVLAMG

ADKNGTVGSV

HAITXYDLKV

VYLEVERXKT

GDVLSYE2MTW QFTI SWDDA(

IKTN'IVVNHI

S ISDKLDVKH XDAI-I1ThENK

EKI'VIVPPTP

RRKETK

TTNAQQPTVG

AVDEVKKDPN

IAKENAEIAA

KGGKF IKATD

NKPVTVTYTG

KDVKTRLTIK

NGSYVKKQAD

TrKGKSNVPV

KEVTDGQKNX

VKVYQADASL

YKYLLVLPFV

SLHDKDIPLQ

GXKTLKAGTO

GDVE'JTQTEN

DLKGYDKDFA

GTVTXSAKDP

PKVXPKKDVV

DKFSGQWSVF

NVAHSWKAFI

KTPQAP\TEPL

EF062-3 (SEQ ID NO:235) TGAITCTTCA AGCAACAAAT GAAAGCAAAA AAACAATATA AGACATATAA AGCTAAGAAT CACTGGGTAA CTGTCCCTAT TCT=FTCTA AGTGTGTTAG GAGCCGTAGG ATTAGCTACT 139 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

S.

S S

S

*SS.

.555

S

S*.

*SS.

5S5* S S *5

GATAATGTAC

AACCCCGACC

GTACAAAAAG

GGTACTGAAC

GGAGCTGAAA

CTAGGGCACC

CCTAAAGAAT

AACATTACCG

GCTGCAAAAG

CAAGCAGCTG

GCAAAAAACA

AAGAACGAAA

GTCGTGACGA

GAT'I=AATA

ATCGGNGGA

TCTAkATGGTG

GGACTAAACG

CAATCCTCAC

ACNGCTT'ITA

AAGTTCT=G

GCGCTGTCTT

GA ITTTCGGG

GATGGAAAAT

AATAGTGATT

GCNAATGGNC

GTATCTAGTG

ATTTTCAATT

AAAGCCAATG

NTCAATCT

ACAGAACTTG

GATGCAGAAA

CTNAACCANA

GTGACTGCTT

AACGCNCACG

GTAGTGAAAA

GAAACGGTAA

AAAGCAGATA

CAAACAAAAA

ACNGAAGAAT

TGGCACGCTA

GATATTTCTG

AATCAAGCAT

TCTGTGTATC

AACTACAACA

AAACCAACCA

CGTGGTGATG

GCCTTTGATA

AANGiTCACAC

AACCAGTTCA

CCACA ACCCT

CTCAAAGCCC

CGAATTAAAA

GTTATTAAAC

TTC LTCTATG

AAGCCGCGGA

TGCAGTCAGA

ACACTACTTC

AAAGTTCAGC

AATCAGCACA

CAACAGAACT

TGAAACACC

'ITGTTGAAAA

AAAAAGAAGT

AATI'AAAAGC

AAGCNGAAAA

ACAGCTATGT

AAGACACTAA

AAGTAAATGC

AAGNTACTC

GGTATrGCCCT CTAGTTJWI7T

CAAGCCAAAO

TTGGTACAAA

ATGCGTCAGG

CTI'TAAATTC

CNAACAATGC

TI'ACTCACC

GGGACGCTGT

GTATTTCCTT

CGCAATGGTT

ATGGGAATCC

TCGTTCCTGT

TAAATGTGAA

CCAAAGTAGA

AAGTGACGAT

AAGACTNAAA

CTTATGANCT

GNTCNGTTGT

ATGTAGAAGG

CAAATACAGT

AAAACGGTAC

TTTATTATGA

GGGGCATGAA

TTACNAANTA

CCTACATI

TATF'GGCNGC

TGGAAGTCGP

AAGAGCTTGCI

AAGCCGTTCP

TTC=ITCTTP

CAGTCGATCO

CAATCAAAG;

CGATCTCNTC

TTATTCTAGC

ATGTTI'CTC

CCAATACNGI

TNGCTGACA)

AATTTACAAC

ATTAGATACG C AAAGGAAACA C

TCAACCGACC

TACCCCAAAT C

AGAACA.ACCAC

TGCACCAGCT

AGACGTTGAT

ACCAGCAGAA

AGACCAACTA

CAAAAATGAA

AGAGCGNTAN

CAATGAAGCG

AATTTCGTCG

AGGGGATTCA

CAACTTCCAG

TCTTTTAGAA

AGGACGTAAA

TGGAACG I A

CAGAGTCAAT

TAAAGAAGTA

AAGTTTAACG

GTTCAAATAC

GGAAGATAIT

AGGTCACAAG

TTCTTTTGGT

TGCCTTTAGN

AAAAGAACCA

NCTTGTGCCN

NCGTGGCGAT

TCCAAAAACA

TGATTTATCC

AGCTGTTGCT

CAATTTAGAT

TI'AGCAATG

CGAT'LTTGA-A

GATTAAC CAT

AGTTGGCAGT

*AGTCAAATCT

*TGATGTCTTG

TGACCTTAAA

TITAGAAA-AC

NTTAAATGAA

ACGGATNAAA

NCGTTCTAAT

LTAACAAGAAA

TGAAATGACN

7TGCGACAGrOC

CTTACTTCGT

GGACGATG-CC

GtIATGGTGGG NGAMT TAT

STGTCAACCAT

kACAAAGTCAA

;TAGCACATT

:AACCAGAAA C :CTAAAACGG C

LZAAGTAGAAG

;ATACCACAA Ja 'TACTAAGCC C .AAAATGAAC 9J k.AAGCAG~rG 3ACTTAGGCA I

AAAAAGAAC

\AAATTGCCA

aA1AAAGAAG

CTTAGTAAAA

ATTAAA-GGCGC

AAAGATATCT'

AATTCC TrG

AAAAATAAAC

ATTACAAAAG

P.ATGCAGTAT

GGTAAGGATG

CTACCAGATA

AATAAAGGTG

ATAATGGNT

GACTATGGCA

AATGCCTACT

ATGACAACAA

ACTAACTTAA

GAAAAAGCAA

AATAAAGAAG

TCTTI'ACAAT

GTAACNA.AAC

AAArGTGAA.AG GCAGCNATrA

CAAAACACCG

GGGTATAAAT

AATACAGCr

GTGCCAGGTA

GTTTCTCTAC

TCCGAACGTC

GACACGACCC

GTAGGGGANA

AAACACAATA

GGAAGCA.ATA

ACAGGTGACG

ACNGTGGTGA

GGGGAAGANA

TGGGACTTAA

GTTTCTTTCT

GTCAAACAT1'

AAAGGCACGG

CAAGAATTGC

AA EWCAGCGG

AITCCAAAAG

AATGGNGCCA

CCTGCAGAAT

:AACGACGGT TI :AGTATCTGA A

LAGTAGCG--C

~CGCGCAACA A :TGAAACAAC C rGAATAAATC

MTAAGTAAA

~CGTTTCT1'C AGCAMAAA C kAGAAAATGC CCGCNGAATA kCCTAGTGTT

;AAAATTTAT

E'TACAAAATT

EAAAAGAGGC

AGTGACAGT

:AGAA--TTGT

UTTCAAACGA

TTAAAACACG

kAGATAGTCC

GCCATGCGGA

CNTATGTGAA

:AGGACCTTC

TTGGIWCAGG

AAGGAAAAAG

ATGCGCAATC

CGATGAATT

TCACTGATGG

ACATTGT--AC

AAGGGATTCG

TTTATCAAGC

ATTCAGGAAN

TCACAGC'AAT

ATTTACT

TTCAGCTGAC

GTAATCCTTC

ATGATAAAGA

CAGCNAACTA

ATGATCGTTT

AAACGTTAA-A

AAGACTrGAC

AACTAGGCAA

TAGAAAACAC

CGCATACNCC

TTAANCATGG

AAGGGTACGA

TCGATGATTA

CTAAAGCGGN

TGACNATNTC

GTrGTAACNCT

AACAAAATAC

TGAANCCTAA

CAATCAAATT

ACGCTGGT

CAACCCAAT

IGAAGCAACA

LGAAAATAAA

~CCAACAGTA

:AATGAACCT

LACGTCCAT

LAAAGATCCA

.AAAGATTTA

ATTGCCCAA

IGAA.ATTGCG

:AACAAGCAT

GATCAATCT

:AAAGCAACT

%CGGAAkAGAT

%AATCTTGGG

'ACCTATACA

TTATGAACTA

rCCGATTATC

CTTAACGATT

AT'IGCGTAT

ATTGTTTCT

PAAACAAGCG

TGGATTGAAA

TGTAGGTCTA

TAATGTGCCT

AGTGAAGCCT

CAATNGATAC

NCAGAAAAAT'

AGGGGATACG

AGATACN'TT

AGACGCAAGT

AGCTAAAGAC

GATGAAAACC

CTTGCCGTT'

AAANGATGGN~

CAAAGATGTA

TATTCCG~rA

TGGCGGAATN

CACAGGNAAA

AGCAGGAACA

GTTTACNATG

ACAAGCTTGG

GCAAACAGAA

TGATGATCCA

AAAAGTNGCT

TAAAGACTTT

CGATGAAACG

AGACATTACG

TOccAAAGAC

CCCTACAAAA

ATITGGNCAA

MAAAGACGTG

AGGGGAGAAN

TGTGGAAGAA

140 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

TG;GTCGATTA

TI'TGCCAATT

TCGAAACTAT

TT=FNGATG

ATTGGTGTAG

AATGAGAAGA

CCAGAAAAA

TTAGTGGTAG

GCGATAAACT AGACGTCAAA CTAATrTGT 'FTTAGCAGAC TCACGATGAC CTTTGAACAA CGATGAATCT AAAAGAAAAC AACGAATTGC GGCAGGAGAC TTAAA-ACNAA TACGCTACTG CAGTGATTGT ACCACCAACA AAAAGGCAAG Tr,

CATGACAA.AT

GGAACCAAAG

GGGGTAGTGA

AAAACGTTG

GTIACAACA

ACNCATACGC

CCAAAA.ACAC

TI'AGTC-CCCA

TGAATAAAGG

AAATCACGC

CACAVI'CATG

CAATCGAAGA

CAGA.AAAACC

CGCAACCACC

ATGGTCTG

GGACGACATT

CAGTCAAGCC

GAAAGCGTTC

ATCTTTCAAC

ACAAACNCCA

AGTAGAGCCA

EF062-4 (SEQ ID NO:236) AELDTQ PET ITVQPNN PDLQSEKETP KTAVSEEATV AEKSAQEQPV VSPETTNEPL ITVVEKPAED LGNVSSKDLA KNKAEXERXX KEVAEYNXHK FNKVNAGDSK DIFTKLRKDM LNASYLGRK I TKAEFVYELQ FFDASGKEVL PDKDSPFAYA GKFYSPEDID YGTCSGLKN SSAQWFAFXT NLNAQSVKPI NDLNVXRGDS LQYIVTGDTT NXKDXKAVAA AINSGXAXDV VK<NVEGDFEN TAVQLTXDGE TKIYYEVKSS ERPANYGGXT I SAY ILLENK DNKDLTFTMN YNKELVRSNT VVTHTPDDFK FDTVDLATGV SFFDDYDETX QAPILAXGGQ ELRVTLPTKV IKVGDKQSQN GATIKLGEXF ANSNFVLADG TKVNKGDDIS CVERIAAGDV YNTIEESFNN

VVEKASV

QKDTTSQPTK

GQPTEVAPAE

AKEKEVDQLQ

NENSYVNEAI

GGKXTGNFQN

SSPSQSGTLN

LSSLNSSLTN

SDWDAVGHKN

FNYGNPKEPE

ELAKVDPKIV

TASYXLNLDQ

'IVTNTVINHV

EEWGMNDVLIJ

QALLAALNEG

PTKAVHNYKKG

VTPIKDLLRV

KADVSGOVYN

FYEFTSSDIP

KLFTMTFEQG

EKIKTNTVVT

VEEVAPENXG

NEVNKSTSIP

KEQAKK IAQQ

SKNLVFDQSV

SFVKEANLGS

AVFSNDPI IT

KGGHAEFVSD

AYFGSGVGLA

KAT IEFNXYK

TKQGIRDTFD

NTVTAMKKTN

PGSNPSKDVK

TTHDRFTGKW

SNKVGKQAW8

EXIXHCKVAR

KDSKGXDITN

SAEQNTFGQR

AEYAGVVEEW

VVKITASQAF

HTPEKPQTPP

TEQSSATPND

KEFETPDVDK

AAELKAKNEK

VTKDTICSSI

NGGYAVLLEK

AF IGTNRVNG FGANNAFKY I NGR ISFSFGM

ANVVPVLVPN

AEKVTIDLSK

ADGSWLAM4G

ADKNGTVGSV

HAIl(YD 4

KV

VYLEVERXYKT

GE)VLSYEMW

QFTISVIDDAI(

IKTNFJVNH I S ISDKLDVKH

XDAMLKENK

EKTVIVPPTP

TTNAQQPTVG

AVDEVKKDPN

IA.KENAEIAA

KGGKF IKATD

NKPVTVTYTG

KDVKTRLTIK

NGSYVKKQAD

TTKGKSNVPV

KEVTDGQKNX

VKVYQADASL

YKYLLVLPFV

SLHDKDIPLQ

GXKTLKAGTD

GDVENTQTEN

DLKGYDKDFA

GTVTXSAKDP

PKVXPKKDVV

DKFSGQWSVF

NVAHSWKAF I

KTPQAPVEPL

EF063-1 (SEQ ID NO:237)

TGATTCTTGA

CACTGGGTAA

GATAATGTAC

AACCCCGACC

GTACAAAAAG

GGTACTGAAC

GGAGCTGAAA

CTAGGGCAGC

CCTAAAGAAT

AACATTACCG

GCTGCAAAAG

CAAGCAGC'rG

GCAAAAAACA

AAGAACGAAA

GTCGTGACGA

GATTTTAATA

AGCAACAAAT

CTGTCCCTAT

AAGCCGCGGA

TGCAGTCAGA

ACACTACTTC

AAACTTCAOC

AATCAGCACA

CAACAGAAGT

TTGAAACACC

TTGTTGAAAA

AAAAAGAAGT

AATTAAAAGC

AAGCNCAAAA

ACAGCTATGT

AAGACACTAA

AAGTAAATGC

GAAAGCAAAA

TCTTTTTCTA

A ITAGATACG

AAAGGAAACA

TCAACCGACC

TACCCCAAAT

AGAACAACCA

TGCACCAGCT

AGACGTTGAT

ACCAGCAGAA

AGACCAACTA

CAAAAATGAA

AGACCGNTAN

CAATGAAGCG

AAITTCGTCG

AGGGGATTCA

AAACAATATA

AGTGTGTTAG

CAACCAGAAA

CCTAAAACGG

AAAGTAGAAG

GATACCACAA

CTACTAAGCCC

GAAAATGAAC

AAAGCAGTIG

GACTTAGGCA

CAAAAAGAAC

AAAATTGCCA

CANAAAGAAG

ATTACTAAAA

ATTMAAGGCG

AAAGATATCT

AGACATATAA

GAGCCGTAGG

CAACGACGGT

CAGTATCTGA

AAGTAGCCC

ACGCGCAACA

CTGAAACAAC

TGAATAAATC

ATGAAGTAAA

ACGI"I'CTTC

AAGCGAAA

AAGAAAATGC

TCGCNiGAATA

ACCTAGTGTT

GAAAATTTAT

TACAAAATT

AGCTAAGAAT

ATIAGCTACT

TCAACCcAAT

AGAAGCAACA

AGAAAATAAA

ACCAACAGTA

CAATCAACCT

AACGTCCATT

AAAAGATCCA

TAAAGATTTA

GATTGCCCAA

AGAAATTGCG

CAACAAGCAT

CGATCAATCT

CAAAGCAACT

ACGGAAAGAT

141 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

a.

ATGGGNGGGA

TCTAATGGT'G

GGACTAAACG

CAATCCTCAC

ACNGCI'rTTA AAGTTrCTTTG

GCGCTGTCTT

GATTTTGGGC

GATGGAAAAT

AATAGTCATT

GCNAAT13GNC

GTATCTAGTG

ATTTTCAATT

AA.AGCCAATG

NTCAATGATT

ACAGAAC LTG

GATGCAGAAA

CTNAACGANA

GTCACTGCCT

AACGCNGACG

GTAGTGAAAA

GAAACGGTAA

AAAcCAGATA

CAAACAAAAA

ACNGAAGAAT

TGGCACGCTA

GATATTTCTG

AATCAAGCAT

TCTCTGTATC

AACTACAACA

AA.ACCAACCA

CGTGGTCATG

GCCTT~FGATA

AANGTGACAC

AACCAGTTCA

CCACAAGCCT

GTCAAAGCCG

CCAATTAAAA

GTTATTAAAG

TTCTTCTATG

TCGTCCA'ITA

TTTGCCAATTI

TCGAAACTAT

T TTTT-NGATC A ITGGTrGTAG

AATGAGAAGA

CCAGAAAAAA

TTACTGGTAG

GTCTTATTAA

AAACGTAGAA

AAGNTACTGG

GGTATGCGGT

CTAGTTATT

CAAGCCAAAG

TI'GGTACAAA

ATGCGTCAGG

CTTTAAATTC

CNAACAATGC

FI'TACTCACC

GCCACGCTGT

GTATTTCCTT

CGCAATGGTT

ATGGGAATCC

TCGITTCCTGT

TAAATGTGAA

CCAAAGTAGA

AAGTIGACGAT

AAGACTNAAA

CTTATGANCT

GNTCNGTITGT

ATGTAGAAGG

CAAATACAGT

AAAACGGTAC

TTATTATGA

GGGGCATGAA

TTACNAANTA

CCTACATTCT

TATTGGCNGC

TGGAAGTCGA

AAGAGCTTGT

AAGCCGTTCA

TTCTI'CTTA

CAGTCGATCT

CAATCAAAGA

CGATCTCNTG

TTATT-:TAGC

ATGTTCTGG

CCAATACNGT

TNGGTGACAA

AATTTACAAG

GCGATAAACT

CTAAT=TGT

TCACGATGAC

CGATGAATCT

AACGAATTGC

TTAAAACNAA

CAGTGATTGT

AAAAGGCAAG

CGGTAGCTGG

AAGAAACAAA

CAACTTCCAG

TCTTTTAGAA

AGGACGTAALA

TGGAACG1'TA

CAGAGTCAAT

TAAAGAAGTA

AAGTTTAACG

GTTCAAATAC

GGAAGATATT

AGGTCACAAG

TTCTTITTGT

TGCC=TAGN

AAAAGAACCA

NCTI'GTGCCN

NCGTGGCGAT

TCCAA.AAACA

TGATI'TATCC

AGCTGTTGCT

CAA ITTAGAT

=~AGCA.ATG

CGATTTTGAA

GATTAACCAT

ACGFrGGCAGT

AGTGAAATCT

TGATGTCTTG

TG3ACCTTAAA

TTTAGAAAAC

NTTAAATGAA

ACGGATNAAA

NCCTTCTA.AT

TAACAAGAAA

TGAAATGACN

TCGACAGGC

C ITACTI'CGT

GGACGATGCC

GNATGGTCGC

NGATGTII'AT

TGTCAACCAT

ACAA.AGTCAA

TAGTGACATT

AGACGTCAAA

TTTAGCAGAC

CTTrGAACAA

AAAAGAAAAC

GGCAGGAGAC

TACGGTAG

ACCACCAACA

TGTNGTGCCA

TAGTTTAGCC

ATAA

AATTCCTTG

AAAAATAAAC

ATTACA-AAAG

AATQCAGTAT

GGTAAGG-ATG

CTACCAGATA

AATAGGTG

ATAATGCNT

GACTATGGCA

AATGCCTACT

ATGACAACAA

AKCTAACTTAA

CAAAAAGCAA

AATAAAGAAC

TCTTTACAAT

GTAACNAAAC

A.AAGTGAAAC

GCAGCNATTA

CAAAACACCG

GGGTATAAAT

AATACAGCTC

GTGCCAGGTA

CTTTCTCTAC

TCCGAACGTC

GACACGACCC

GTAGGGGANA

AAAGACAATA

GGAAGCAATA

ACAGGTGACC.

ACNGTGGTGA

GGGGAAGA'A

TGGGACTTAA

GTTTCTTCT

GTCAAAGA'T

AAAGGCACGG

CAAGAATTC

AATTCAGCCG

ATITCCAAAG

AATGGNGCCA

CCTGCAGAAT

CATGACAAAT

GGA.ACCAAAG

GGGGTAGTGA

AAAAACG1rG

GTTTACAACA

ACNCATACGC

CCAAAAACAC

GAATTGCCGC

CCAATCc'r

TAAA-AGAGGC

CAGTGACAGT

CAGAA=rGT

TTTCAAACGA

TTAAAACACG

AAGATAGTCC

GCCAMCGGA

CNTATCTGAA

CAGGACCTTC TGGATTGAAA TTGGTTCAGG TGTAGGTCTA A.ACCAAAAAG TAATGTCCCT ATGCGCAATC AGTGALAGCCT CGATTGAATT CAATNGATAC TCACTGATGG NCAGAAAAAT ACATPMTGAC ACGGGATACC AAGGGATTCG ACATACNTTT TTTATCAACC AGACGCAAGT ATTCAGGAAN ACCTAAAGAC TCACAGCALAT GATGAAAACC AT'LTACTTGT C'IrGCCGTTT TTCAGCTGAC AAANGATGGN GTAATCCTTC CAAAGATGTA ATGATAAAGA TATCCGTTA CAGCNAACTA TGGCGGAATN ATGATCGTTT CACAGGNAA-A AAACGTTAAA AGCAGGAACA A.AGACTTGAC GTTTACNATG AAGTAGGCAA ACAAGCTTG TAGAAAACAC GCAAACAGAA CGCATACNCC TGATGATCCA TTAANCATGG AAAAGTNGCT AAGGGTACGA TAAAGACTTT TCGATGATTA CGATGAAACG CTAA-AGCGGN AGACATTACG TGACNATNTC TGCCAAAGAC GTGTAACNCT CCCTACAA;AA AACAAAATAC AITI'GGNCAA TGAANCCTAA AAAACACGTG CAATCAAATT AGGGGAGAA-N ACGCTGGNCT TC-TCGAAGAA TTAGTGGCCA ATGGTCTGTG TGAATAAAGG GGACGACATT AAATCACGG;C CAGTCAAGCC CACACTCATG GAAAGCGTTC CAATCGAAGA ATCTITCAAC CAGA.AAAACC ACAAACNCCA CGCA.AGCACC ACTAGAGCCA AAACAGGCGA AAAACAAAAT GCTTAGCAGG; CTTAGGcTITT

AAATCTTGGG

GACCTATACA

TI'ATGAACTA

TCCGATTATC

CTTAACGATTr

ATTTGCGTAT

ATTTGTTTCT

AAAACAACCC

EF063-2 (SEQ ID NO:238) 142 TABLE 1. Nucleotide and Anino Acid Seqeuences of E. faeca/is Genes.

MKAKK QYKTYKAKNH WVTVPILFLS VLGAVGLATD NVQAAELDTQ PETTTVQPNN

PDLQSEKETP

AEKSAQEQPV

ITVVEKPAED

KNKAEKERXX

FNKVNAGOSK

LNASYLGRKI

FFDASGKEVL

GKFYSPEDID

SSAQWFAFXT

NDLNVXRCDS

NXKDXKAVAA

VKNVEGOFEN

TX IYYEVKSS

ISAYILLENK

YNXELVRSNT

FDTVDLATCV

QAFILAXGGQ

IKVGDKQSQN

ANSNFVLADG

GVER IAAGDV

VVEKASVVPE

KTAVSEEATV

VSPETTNEPL

LGNVSSKDLA

KEVAEYNKHK

DIFTKLRKDM

TKAEFVYELQ

PDKDSPFAYA

YGTGPSGLKN

NLNAQSVKP I LQY IVTGDTT

AINSGXAKDV

TAVQLTXDGE

ERPANYGGXT

DNKDLTFTMN

VVTH-TPDOPK

SFFDDYDETX

ELRVTLPTKV

GATIKLGEXF

TKVNKGDOI S

YNTIEESFN

LPQTEKQNV

QKDTTSQPTK

GQPTEVAPAE

AK:EKEVDQLQ

NENSY-VNEAI

GGKXTGNFQN

SSPSQSCTLN

LSSLNSSLTN

SDWDAVGHKN

FNYGNPKEPE

ELAKVDPKTV

TASYXLNLDQ

TVTNTVINH-V

EEWGMNDVLD

QALLAALNEG

PTKAVHNKKG

VTPIKOLLRV

KADVSGDVYN

FYEFTSSDIP

KLFTMTFEQG

EK IKTNTVVT

LLTVAGSLAA

VEEVAPENYG

NEVNKSTSIP

KEQAKKIAQQ

SKNLVFEDQSV

SFVIKEANLGS

AVFSNDPI IT

KGGRAEFVSD

AYFGSGVGLA

'ATIEFNXYK

TKQGIRDTFO

fTVTAMTN

PGSNPSKDVK

TTHDRFTGKW

SNKVGKQAWS

EXIXHGKVAR

KDSKGXDITN

SAEQNTF'GQR

AEYAGVVEEW

VVKITASQAF

TE-QSSATPND

KEFETPDVDK

AAELKAKNEK

VTKIDTKISSI

NGGYAVLLEK

AFIGTNRVNG

FGANNAFKYI

NGRISFSFGM

ANVVPVLVPN

AEKVTIDLSK

ADGSVVLAMG

ADKNGTVGSV

HAITXYIJLKV

VYLEVERXKT

GDVLSYEMTW

QFTI SWDDA( IKTN'IVVNH I

SISDKLDVKH

XDAMNLKENK

TTNAQQPTVG

AVDEVKKDPN

IAKENAE IAA

KGGKFIKATD

NKPV7VTYTG

XDVKTRLTIK

NGSYVKKQAD

TTKGKSNVPV

KEVTDGQ-KNX

VKVYQADASL

YKYLLVLPFV

SLHDKDIPLQ

GXKTLKAGTD

GDVENTQTEN

DLKGYDKDFA

GTVTXSAKDP

PKVXPKKEDVV

DKFSGQWSVF

NVAHSWKAFI

KTPQAPVEPL HTPEKPQTPP EKTVIVPPTP MLGLAGLG:FK RRKETK EF063-3 (SEQ ID N'O:239) GGA ATTAGATACG CAACCAGAAA CAACGACGGT TCAACCCAAT

AACCCCGACC

GTACAAAAAG

GGTACTGAAC

GGAGCTGAAA

CTACGCAGC

CCTAAAGAAT

AACATTACCG

GCTG.CAAAAC

CAAGCAGCTG

GCAAAAAACA

AAGAACGAAA

GTCGTGACGA

GAI=TAATA

ATGGGNGGGA

TCTAATGGTG

GGACTAAACG

CAATCCTCAC

ACNGCTTTTA

AAGTTCTTTG

GCGCTGTCTT

GATTTGGGG

GATGGAAAAT

AATAGTGATT

GCNAATCGNC

GTATCTAGTG

ATTTTCAATT

TGCAGTCAGA

ACACTACTTC

AAAGTTCAGC

AATCAGCACA

CAACAGAAGT

TTGAAACACC

TGTTGAAAA

AAAAAGAAGT

AATTAAAAGC

AAGCNGAAAA

ACAGCTATGT

AAGACACTAA

AAGTAAATGC

AAGNTACTGG

GGTATGCGGT

CTAGTTATTTI

CAAGCCAAAG

TTGGTACAAA

ATGCGT'CAGG

CTTTAAATI'C

CNAACAATGC

TTTACTCACC

GGGACGCTGT

CTATTTCCTT

CGCAATGGTT

ATGGGAATCC

AAAGGAAACA

TCAACCGACC

TACCCCAAAT

AGAACAACCA

TGCACCAGCT

AGACG ITGAT

ACCACCAGAA

AGACCAACTA

CAAAAATGAA

AGAGCGNTAN

CAATGAAGCG

AATTTCGTCG

AGGGGATI'CA

CAACTTCCAG

TCTTTTAGAA

AGGACGTAAA

TGGAACGTTA

CAGAGTCAAT

TAAAGAAGTA

AAGTTTAACG

GTTCAAATAC

GGAAGATATT

AGGTCACAAG

CCTAAAACGG

AAAGTAGAAG

GATACCACAA

GTAGTAAGCC

GAAAATGAAG

AAAGCAGTTG

GACTI'AGGCA

CAAAAAGAAC

AAAATTGCCA

GANAAAGAAG

ATTAGTAAAA

ATTAAAGGCG

AAAGATATCT

AATTCCTTTG

AAAAATAAAC

ATI'ACAAAAG

AATGCAGTAT

GGTAAGGATG

CTACCAGATA

AATAAAGGTG

ATTAATGGNT

GACTATGGCA

AATGCC TACT

CAGTATCTGA

AAGTAGCGCC

ACGCGCAACA

CTGAAACAAC

TGAATAAATC

ATGAAGTAAA

ACGTTI'CTTC

AAGCGAAAAA

AAGAAAATGC

TCGCNGAATA

ACCTAGTGT

GAAAA TIAT TTACAAAA T

TAAAAGACGC

CAGTCACAGT

CAGAA'TTGT

TTCAAACGA

TI'AAAACACG

AAGATAGTCC

GCCATGCGGA

CNTAT13TGAA

CAGGACCTTLC

TI'GGTCAGG

AAGGAAAA.AG

ATrCCGCAATC

CGATTGAATT

AGAAGCAACA

AGAAAATAAA

ACCAACAGTA

CAATGAACCT

AACGTCCT

AAAAGATCCA

TAAAGATTTA

GA'ITGCCCAA

AGAAATTC

CAACAAGCAT

CGATCAATCT

CAAAGCAACT

ACGGAAAGAT

AAATCTTGGG

GACCTATACA

TTATGAACTA

TCCGATTATC

CTTAACGATT

ATTTGCGTAT

ATTTGTI'TCT

AAAACAACCG

TGGAITGAAA

TGTAGGTCTA

TAATGTGCCT

AGTGAAGCCT

CAATNGATAC

TTCrTrGGT ATCACAACAA MCCTITAGN ACTAACTTAA AAAAGAACCA GAAAAAGCAA 143 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

AAAGCCAATG TCGTTCCTGT NCT'TGTGCCII AATAAAGAAG NTCAATGATr TAA.ATGTGAA NCG TGGCGAT TCTT'FACAAT ACAGAACTTG CCAAAGTAGA TCCAAAAACA GTAACNAAAC GATCCAGAAA AAGTGACGAT TGATTTATCC AAACTG

TCACTGATGG

ACATTTGAC

AAGG;GATTCG

NCAGAAkAAAT

AGGGGATACG

AGATACNFT

EF063-4 (SEQ ID NO:240) ELDTQ PETVQPNN PDLQSEKETP KTAVSEEATV AEKSAQEQPV VSPETTNEPL ITVVEXPAED LGNVSSKDLA KNKAEKERXX KEVAEYNKHK FNXVNAGDSK DIFTKLRKDM LNASYLGRXI TKAEFVYELQ FFDASGKEVL PDKDSPFAYA CKFYS PEDID YGTGPSGLKN SSAQWFAFXT NLNAQSVKP I NDLNVXRGDS LQYIVTGDTT

QKDTTSQPT(

GQPTEVAPAE

AKEKEVDQLQ

NENSYVNEAI

GGKXTCN'FQN

SSPSQSGTLN

LSSLNSSLTN

SDWDAVGHKN

FNYGNPKEPE

ELAKVDPKTV

VEEVAPEN(G

NEVNKSTSIP

KEO2AKK IAQQ

SKNLVFDQSV

SFVKEANLGS

AVFSNDPIIT

KGGHAEFVSD

AYFGSGVGLA

KATIEFNXYK

TKQGIRDTFD

TEQS SATPND

KEFETPOVDK

AAELKAKNEK

VTKDTKISS I

NGGYAVLLEK

AF IGTNRVNG

FGANNAFKYI

NGRI SFSFGM

ANVVPVLVPN

AEKVTIDLSK

TTNAQQPTVG

AVDEVKKDPN

IAKENAETIAA

KGGKF IKATD

NI(PVTVTYTG

KDVKTRLTIK

NGSYVKKQAD

TTKGKSNVPV

KEVTDGQKNX

EF064-1 (SEQ ID NO:241)

C..

C.

TCATTCTTGA

CACTGGGTAA

GATAATGTAC

AACCCCGACC

GTACAAAAAG

GGTACTGAAC

GGAGCTGAAA

CTAGGGCAC

CCTAAAGAAT

AACA'ITACCG

GCTGCAAAAG

CAAGCAGCTG

GCAAAAAACA

AAGAACGAAA

GTCGTGACGA

GA'I=TAATA

ATGGGNCGGA

TCTAATGTG

GGACTAAACG

CAATCCTCAC-

ACNGC=TTA

AAGTI'CTTTG

GCGCTGTCTT

GATTTTGGGG

GATGGAAAAT

AATAGTGATT

GCNAATGGNC

GTATCTAGTG

ATICAT

AAACCCAATG

NTCAATGATT

ACAGAACTI'G

ACCAACAAAT

CTGTCCCTAT

AAGCCGCGGA

TGCAGT-CAGA

ACACTACTTC

AAAGTTCAGC

AATCAGCACA

CAACAGAACT

TTGAAACACC

T

TGTTGAAAA

AAA.AAGAIAGT

AATTAAAAGC

AAGCNGAAAA

ACACCTATGT

AAGACACTAA

AAGTAAATGC

AAGNTACTGG

GGTATCCGGT

CTAGTTATT

CAAGCCAALAG

T1'CGTACAAA

ATGCGTCAGG

CTI'TAAATTC

CNAACAATC

TTTACTCACC

GGGACGCTGT

GTATTTCCT

CGCAATGGTT

ATGGGAATCC

TCGTTCCTCT

TAAATGTGAA

CCAAAGTAGA

GAAAGCAAAA

TCT'ITTTCTA

ATTAGATACG

AAAGGAAACA

TCAACCGACC

TACCCCAAAT

AGAACAACCA

TGCACCAGCT

AGACGTTGAT

ACCAGCAGAA

AGACCAACTA

CAAAA.ATGAA

AGAGCGNTAN

CAATGAAGCG

AATTCGTCG

AGGGGATTCA

CAACTTCCAG

TCTTTTAGAA

AGGACGTAAA

TGGAACGTTA

CAGAGTCAAT

TAAAGAAGTA

AAGFI 'AACG

GTTCAAATAC

GGAAGATT

AGGTCACAAG

TICTTTGT

TGCC IT'AGN

AAAAGAACCA

NCTTGTCCCN

NCGTGGCGAT

TCCAAAAACA

AAACAATATA

AGTCTGTTAG

CAACCAGAAA

CCTAAAACGG

AAACTAGAAG

GATACCACAA

GTAGTAAGCC

GAAAATGA.AG

AAAGCAGTTG

GACI'AGGCA

CAAAAAGAAC

AAAATTGCCA

GANAAAGAAG

ATTIACTAAAA

ATTIAAAGGCG

AAAGATATCT

AATTCCTTTG

AAAAATAAAC

ATTACAAAAG

AATGCAGTAT

GrGTAAGGATG

CTACCAGATA

AATAAAGGTG

ATTAATGGNT

GACTATGGCA

AATGCCTACT

ATGACAACAA

ACTAACTTAA

GAAAAAGCAA

AATAAAGAAG

TC=IACAAT

GTAACNAAAC

AGACATATAA

GACCCCTAGG

CAACGACGGT

CAGTATCTGA

AAr-TAGCGCC

ACGCGCAACA

CTGAAACAAC

TGAATAAATC

ATGAAGTAAA

ACGT=ITTC

AAGCGAAAAA

AAGAAAATGC

TCGCNGAATA

ACCTAGTGTT

GAAAATI'TAT

TTACAAAATT

TAAAAGAGGC

CAGTCACAGT

CAGAATTTGT

TTTCAAACGA

TTAAAACACG

AAGATAGTCC

GCCATGCGGA

CNTATGTGAA

CAGGACCT'C

TTGGITCAGG

AAGGAAAAAG

ATGCGCAATC

CGATLTGAATrI

TCACTGATGG

ACATTGTGAC

AAGGGATTCG

AGCTAAGAAT

ATTAGCTACT

TCAACCCAAT

AGAAGCAACA

AGAAAATA.AA

ACCAACAGTA

CAATGAACCT

AACGTCCAT'

AAAAGATCCA

TAAkAGATFE'A

GATTGCCCAA

AGAAATTGCG

CAACAAGCAT

CGATCAATCT

CAAAGCAACT

ACGGAAAGAT

AAATCTTGGG

GACCTATACA

TTATGAACTA

TCCGATTATC

CTAACGT

ATI'GCGTAT

ATTT GTTI'CT

AAAACAAC

TGGATI'GAAA

TGTAGGTICTA

TAATGTGCCT

AGTGAAGCCT

CAATNGATAC

NCAGAAAAAT

AGGGGATACG

AGATACNTIT

TABLE 1. Nucleotide and Amnino Acid Seqeucnces of E.faecalis Genes.

GATGCAGAAA AAGTGACGAT CTNAACGANA AAGACTNAAA GTGACTGCTT CTTATGANCT AACGCNGACG GNTCNGTTGT GTAGTGAAAA ATCTAGAAGC GAAACGGTAA CAAATACAGT AAAGCAGATA AAAACGGTAC CAAACAAAAA TTTATTATGA ACNGAAGAAT GGGGCATGAA TGGCACGCTA 'ITACNAANTA GATATTTCTG CCTACATTCT AATCAAGCAT TATTGGCNGC TCTGTGTATC TGGAAGTCGA AACTACAACA AAGAGCTTCT AAACCAACCA AAGCCjTTCA CGTGGTGATG TTCTTTCTTA GCCT=G-AT-A CAGTCGATCT AANGCACAC CAATCAAAGA AACCAGTTCA CGATCTCNTG CCACAAGCCT TTATTCTAGC GTCAAAGCCG ATG =TCTGG CGAATTAAAA CCAATACNGT GTATAAAG TNGGTGACAA TTCTTCTATG AATI'TACAAG TGGTCGATTA GCGATAAACT ITrGCCAATT CTAA'T7T TCCAAACTAT TCACGATGAC TITTNGATG CGATGAATCT ATTGGTGTAG AACGAATTGC

AATGACAAGA*TTAAAACNAA

CCAGAAAAAA CAGTGATTGT

TGATTTATCC

AGCTGTTGCT

CAATTTAGAT

TrTTAGCAATG CGA'TTrCAA

GATI'AACCAT

AGTTGGCAGT

AGTGAAATCT

TGATGTCTTG

TGACCTTAAA

TITTAGAAAAC

NTTAAATGAA

ACGGATNAAA

NCGTTCTAAT

TAACAAGAAA

TGAAATGACN

TGCGACAGGC

CTTACTTCGT

GGACGATGCC

GNATGGTGGG

NGATCTTTAT

TGTCAAC CAT

ACAAAGTCAA

TAGTGACATT

AGACGTCAAA

T'ITAGCAGAC

CT'rrGAACA-A

AAAAGAAAAC

GGCAGGAGAC

TACGGTAGTG

ACCACCAACA

AAAGTGAAAG

GCACCNATTA

CAAAACACCG

GGGTATAAAT

AATACAGCTlr

GTGCCAGGTA

GTTTCTCTAC

TCCGAACGTC

GACACGACCC

GTAGGGGA1NA

AAAGACAATA

GGA-AGCAAT-A

ACAGGTGACG

ACNGTGGTGA

GGGGAAGANA

TGGGACTTAA

G'TTCTTTCT

GTCAAAGATI'

AAAGGCACGG

CAAGAATTC

AATTCAGCGG

ATTCCAAAAG

AATGGNGCCA

CCTGCAGAAT

CATGACAAAT

GGAACCAAAG

GGTAGTGA

AAAAACGTTG

GTTTACAACA

ACNCATACGC

CCAAAAACAC

GAA ITGCCGC

GCAATGCTTG

T=I1ATCAAGC

ATTCAGCAAN

TCACAGCAAT

ATTTACTTGT

F1'CAGCTGAC

GTAATCCTTC

ATGATAAAGA

CAGCNAACTA

ATGATCGTT

AAACGTTAAA

AAGAC=IAC

AAGTACCCAA

TAGAAAACAC

CGCA#ICNCC

TTA.ANCATGG

AAGGGTACGA

TCGATGATTA

CTAAAGGGN

TGACNATNTC

GTGTAACNCT

AACA-AAATAC

TGAANCCTAA

CAATCAAATT

ACCCTGGNGT

TTAGTGGCCA

TG.AATAAAGG

AAATCACGC

CACACTCATG

CAATCGAAGA

CAGAAAAACC

CGCA.AGCACC

AAACAGGCGA

GCTTAGCAGG

AGACGCAAGT

ACCTAAAGAC

GATGAAAACC

CTTGCCGTTT

AAANGATGGN

CAAAGATGTA

TATTCCGTTA

TGGCGGAATN

CACAG4GNA.AA

AGCAGGAACA

G'TTACNATG

ACAAGCTTGG

GCAAACAGAA

TGATGATCCA

AAAAGTNGCT

TAAAGACTT

CGATGAAACG

ACACA'ITACG

TGCCAAAGAC

CCCTACAAAA

ATTTGGNCAA

AAAAGACGTG

AGGGGAGAAN

TGTGGAAGAA

ATGGTCTGTG

GGACGACATT

CACTCAACCC

GAAAGCGTTC

ATCT PTCAAC

ACAAACNCCA

AGTAGAGCCA

AA.AACAAAAT

CTAGGCrTTT 4 4

C.

4 4 T1'AGTGGTAG

GTCTTATTAA

AAACGTAGAA

AAAAGGCAAG TGTNGTGCCA CGGTAGCTGG TAGTTTACC AAGAAACAAA ATAA EF064-2 (SEQ ID NO:242) MXAKK QYKTYKAKNH WVTVPILFLS VLGAVGLATD NVQAAELDTQ PE-TTVQPNN 4 C. 4 .4

S.

PDLQSEKETP

AEKSAQEQPV

ITVVEKPAED

KNKAEKERXX

FN](VNAGOSK

LNASYLGRKI

FFDASGKEVL

GKFYSPEDID

SSAQWFAFXT

NDLNVXRGDS

NXKDXKAVAA

VKNVEGDFEN

TKIYYEVKss I SAY ILLENK

YNKELVRSNT

KTAVSEEATV

VSPETITNEPL

LGNVSSKDLA

KEVAEYNKH(

DIFTKLRKDM

TKAEFVYELQ

PDKDSPFAYA

YGTGPSGLKN

NLNAQSVKP I

LQYIVTGDTT

AINSGXAKDV

TAVQLTXDGE

QKDTTSQPTI(

GQPTEVAPAE

AXEXKEVDQLQ

NENSY-VNEAI

GGKXTGNFQN

SSPSQSGTLN

LSSLNSSLTN

SDWDAVGHKN

FNYGNPKEPE

ELAKVDPKTV

TASYXLNLDQ

TVTNTVINHV

VEEVAPEN(C

NEVNKSTSIP

KEQAYKKIAQQ

SXNLVFD)QSV

SFVKEANr~LGS AVFSNDP IIT

KGGHAEFVSD

AYFGSGVGLA

KATIEFNXYK

TKQGIRIDTFD

NTVTAMMdXTN

PGSN'PSKDVK

TTHDRFTGI(W

SNXVGKQAWS

EX IXHGKVAR TEQSSATPND ITNAQQPTVG KEFETPDVJDK AVDEVKKDPN AAELKCAKNEK IAKENAEIAA VTXDTKISS I KGGKFIKATD NGGYAVLLEK NKPVTVTYTG AF IGTNRVNG KDVKTRLTIK FGANNAFKYI NGSYVKKQAD NGRI SFSFGM TTKGKSNVPV ANVVPVLVPN KEVTDGQKNX AEKV'T-DLSK VKVYQADASL ADCSVVLAMC YKYLLVLPFV ADKNGTVGSV SLI.DKD IPLQ HAITXYDLKV GXKTLKAGTD VYLEVERXKT GDVE.NTQTEN GDVLSYEMTW~ DLKGYDKDFA ERPANYGGXT EE'WCMNDVLD DNKDLTFTMN QALLAALNEC VVTHTPDDPK PTKAVHNKXG 145 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalisv Genes.

FDTIVDLA G-V QAF IL.XGGQ

I(VGDKQSQN

ANSNFVLADG

GVER IAAGDV

VVEKASVVPE

SFFDDYDETX

ELRVTLPTKV

GATIKLGEXF

TKVNKGDDIS

YNTIEESFNN

LPQTGEI{QNV

VTPIKDLLRV

KADVSGDVYN

FYEF'TSSDIP

KLFTMTFEQG

EKIKTNTVVT

LLTVAGSLA-A

KDSKGXD ITN

SAEQNTFGQR

AEYAGVVEEW

VVI(ITASQAF

HiTPEXPQTPP

MLGLAGLGFK

QFTISWDOAX

I KTNTVVNH4 I

SISDKLDVKH

XDAMNLKENK

EKTVIVPPTP

RRKETK

GTVTXSAKDP

PKVXPKXDVV

DKFSGQWSVF

NVAHSW1(AF I

KTPQAPVEPL

EFO64-3. (SEQ ID NO:243) AGTGACGAT TGATATCC AAAGTGAAAG IT=ATCAAGC AGACGCAAGT a a.

a p a a *aa.

CTNAACGANA

GTGACTGCTT

AACGCNGACG

GTAGTGAAAA

GAAACGGTAA

AAAGCAGATA

CAAACAAAAA

ACNGAAGAAT

TGGCACGCTA

CATATTTCTG

AATCAAGCAT

TCTGTGTATC

AACTACAACA

AAACCAACCA

CGTGGTGATG

GCCTTTGATA

AANGTCACAC

AACCAGTTCA

CCACAAGCCT

GTCAAAGCCG

CGAATTAAAA

GTTATI'AAAG

TTCTTCTATG

TGGTCGATA

TTTGCCAATT

TCGAAACTAT

TITI'NGATG

ATTGGTGTAG

AATGAGAAGA

CCAGAAAAAA

?TAGTGGTAG

GTCTTAI'AA

AAACGTAGAA

AAGACTNAAA AGCTGTTGCT CTTATGANCT CAATTAGAT GNTCNGTI'GT TFTtArcAATG ATGTAGAAGG CGATTTTGAA CAAATACAGT GATTAACCAT AAAACGGTAC AGTIGGCAGT =TTATTATGA AGTGAAATCT GGGGCATGAA TGATCTCTTG TTACNAANTA TGACCTTAAA CCTACATTCT TTTAGAAAAC TATTGGCNGC NTTAAATGAA TGGAAGTCGA ACGGATNAAA AAGAGCTTGT NCGTTCTAAT AAGCCGTTCA TAACAAGAAA TTCTTI'CTTA TGAAATGACN CAGTCGATCT TGCGACAGGC CAATCAAAGA CTTACTTCGT CGATCTCNTG GGACGATGCC TTATTCTAGC GNATGGTGGG ATCTTTCTGG NGATGTTTAT CCAATACNGT TGTCAACCAT TNGGTGACAA ACAAAGTCAA AATTTACAAG TAGTGACATT GCGATAAACT ACACGTCAAA CTAATTI'GT TT1AGCAGAC TCACGATGAC CI'TGAACAA CGATGAATCT AAAAGA.AAAC AACGAATTGC GGCAGGAGAC TTAAAACNAA TACGGTAGTG CAGTGA ITGT ACCACCAACA AAAAGGCAAG TGTNGTGCCA CGGTAGCTGG TAGTTTAGCC AAGAAACAAA ATAA GCAGCNATrA

CAAAACACCC

GGGTATAAAT

AATACAGCTG

GTGCCAGGTA

GITTTCTCTAC

TCCGAACGTC

GACACGACCC

GTAGGGGANA

AAAGACA.ATA

GGAACCA.ATA

ACAGGTGACG

ACNGTGGTGA

GGGGAAGANA

TGGGACTTAA

G=TTTCT

GTCAAAGATT

AAAGGCACGO

CAAGAA ITGC

AATTCAGCGG

ATTCCAAAC

AATGGNGCCA

CCTGCAGAAT

CATGACAAAT

GGAACCAAAG

GGGGTAGTGA

AA.AAACGMr

GTTTACAACA

ACN'CATACGC

CCAAAAACAC

GAATTGCCGC

GCAATGCTTG

ATTCAGGAAN

TCACAGCAAT

ATTAC'ITGT

TCAGICTGAC

GTAATCCTTC

ATGATAAAGA

CAGCNAACTA

ATGATCGTIT

AAACG'ITAAA

AAGACTTGAC

AAGTAGGCAA

TAGAAAACAC

CGCATACNCC

TTAAIWCATGG

AAGGGTACGA

TCGATGMAT

CTAAAGGGGN

TGACNATNTC

GTGTAACNCT

AACAAAATAC

TCAANCCTAA

CAATCAAATT

ACGCTGGNGT

TTAGTGGCCA

TGA-ATAAAGG

AAATCACGGC

CACACTCATO

CAATCGAAGA

CAGAAAAACC

CGCA.AGCACC

AAACAGGCGA

G;C IrArCAGG

AGCTAAAGAC

GATGAAAACC

CTTGCCGTTT

AAANGATGGN

CAAAGATGTA

TATTCCGTTA

TGGCGGAATN

CACAGGNAA

AGCAGGAACA

GTTTACNATG

ACAAGCTTGG

CCAAACAGAA

TGATGATCCA

AAAACTNGCT

TAAAGACTTT

CGATGAAACG

AGACATTACG

TGCCAAAGAC

CCCTACAAAA

ATTGGNCAA

AAAAGACGTG

AGGGGAGAAN

TGTGGAAGAA

ATCGTCTGTG

GGACGACATT

CAGTCAAGCC

GAAAGCCTTC

ATCTTTCAAC

ACAAACNCCA

AGTAGAGCCA

AAAACAAAAT

CTTAGGCTT

EF064-4 (SEQ ID 110:244) VTIDLSK VKVYQADASL

NXKDXYKAVAA

VKNVEGDFEN

TKIYYEVKS S

ISAYILLENK

YNKELVRSNT

FDTVD)LATCV

QAFILAXGGQ

AINSGXAKDV

TAVQLITXDGE

ERPANYCCXT

DNKDLTFTMN

VVTHTPDDPK

SFFDDYDETX

ELRVTLPTKV

TASYXLNLDQ

TVTN'IVINHV

EEWGMNDVLD

QALLAALNEG

PTKAVHNKKG

VTP IKDLLRV

KADVSGDVYN

NTVTAMMYKTN

PGSNPSKDVK

TI'NDRFTGKW

SNKVGKQAWS

EXIXHGKVAR

KDSICCXD ITN

SAEQNTFGQR

AD)GSVVLAMG

ADKNGTVGSV

HA ITXYDLK V

V'YLEVERXKT

GDVLSYEMTW

QFTI SWDDA(

IKTNTIVVNHI

YKYLLVLPFV

SLHDKDIPLQ

GXKTLKAGTD

GDVENTQTEN

DLJKGYDKOFA

GTVTXSAKDP

PKVXPKKDVV

146 TABLE 1. Nucleotide and Amino Acid Seqeuences of Efaecalis Genes.

IKVGDXQSQN GATIKLGEXF FYEFTSSDIP AEYAGVV-EEW ANSNFVLADG TKVNKGDDIS KL.FTMTFEQG VVKITASQAF GVERIAACDV YNTIEESFNN EKIKTNTVVT HTPEKPQTPP

VVEKASV

SISDKLDVKH

XDAMNLKENK

EKTV IVPPTP

DKFSGQWSVF

NVAHSWKAF I

KTPQAPVEPL

EF065-1 (SEQ ID NO:245)

S

S

TAGCGAAAGA

ATGGTGA'TTO

GAAGCGGTTA

AGTCTGGCTG

GAAACGGAAC

TTTCCACATG

GTGATGCTGG

ATTAATTCAC

AGCCAATTTG

GGATTGAAAC

ACGTATTTCC

AAGACCAATA

TCAGTGGAAT

GAAATTACTA

AGITCAGTGA

GAGTTGCAAC

TTTACAACCC

GCTI'CATTAA

GGAAATGATG

TACGTAGGAA

ACCCTTGTAA

ACGATTCCTA

ACAAAAGATA

TGGCATGTCA

GATGACATTA

GATGTTACAG

AAACAAGCAG

AAAATTAAAA

CCCAACCAAG

ACCGTAACAC

TTAGATITAA

GAAACAAGCA

ACTGATGTAA

CAACAAAAkTA

AGTGGTCATA

GAATTAGCAC

AACGAAGC

GAAGATCCAA

GACCAAGAAT

CAAGCCAGCA

GANGAAAATG

ACTT'ITACTA

ATGACTATTA

GAACAAGGCG

CATTCCAACA

AAATAGGGAG

TCGCTGGAAC

AAGCAGGAGA

ATI'GTAAACG

CAGTCCAT'N'

TAAGACAAGC

CTTCATATCG

CTGATI'ATGA

TCTCGGTTT

TCGCTTTAGA

TATTAGTGAC

CCAATGATTC

ATAGTAATGA

ACCAAGGCTA

ATTCATACTT

AAGCGGTCTAG

AATTAAAACA

CGATTGCCAA

TGCCTGTTCA

ACATCACGAT

GTAGTGGGAC

AAAATGCAA

TCGAAAATCA

AAkACAGCCTT

ATAAAGTGCT

CTAACGGCAC

ACAGCTATGA

CTGACGCAAC

CCGAC ITAAA

CACCGCCAGT

CCAACCGCGA

CTI'GGACCCA

AAGTCACAGA

ACAAACTAAC

CGTACACAAT

CTATATTGA

ACGTGTTGCA

CGAITACAAA

TTAAATGGAA

TGGTGGATGA

GCAAAGATGT

TGAACAAAAA

CCACTAAAAT

GGATTCCCAA

AGCCAACCGT

GATr-AAAATG

AGTTGTGGGA

TACAGAAGGA

GATATTGGAA

AGTAGTTCTI'

GATTGATGAA

CGGCGGAAAA

TATGAATGTG

TGGAGACGTT

TACGTACAAT

AGATGGGGTC

AATCAATGAA

CTACCAAGGT

TGAAATGATT

TGATAAATAT

CACACCAGAA

AATTGTCAAA

TCAATTTGAT

AATTAACGGA

TCACTACGAA

AATGALATCAA

TGCGCATGCG

AGAACACTTA

TGGCAACGAA

AGATATCATT

AGTAACACAA

CTATI'TAAGT

GGACGAAGAA

CTTTGGCAAT

TGATCCAAAT

TCAAGAATT

AGCCAGCATC

TGAAAATGGT

'FITrGAA.AT

GACCATTACT

ACAAGGTGGC

TTCCAACAAA

AGATATCGAA

CGTCAAAACA

CATTAATAAA

TACAGATAAT

AGATGACAGC

TAAAACTGAC

CCAAGCCGAC

AACACCGCCT

TTTAAGAAAG

A.ATT-CAGTC

ATGACCAATA

GGACAAG CTA

GAAGATGCTA

GTGGTTCAAG

CAATTTATGT

CGCGTCAATA

CGGACGTATG

CAAACACACG

GCTAATACAC

TATCCAGATC

GCAGCAGCAG

AATGCGTATr

AAAACAGAAC

GAT=TATTA

GATCGTCTGG

ATTCAATCTG

CAAACCATT

GTCAAAGAAA

GGAACAATIG

TGTGACGTCA

GATTTAACCA

ACCAGTACTT

GATGTGAAAG

GAAAATAACA

GGTCATACGTr TTAGCGCCTr

GAAGGTGACG

ATTGCTAAAG

AAATGOAACG

GTAGATGACA

AAAGATGTrA

AACAANCAAG

ACTAAA.ATCA

ATTCCCAACC

CCAACCGTAA

GG.CCAA=AC

GCTTrCGGTA

GTGTTAGACA

GGCATAr-TAA

TACTCTTACT

GCAACGGATG

TTAAACTTTG

GCACCA.ACC

CAAC3AAATT

CCACATTG.GC

CGGTGAAAGT

TTICCCAGT

GTGGTAGTTT

GCTTATCTGA

I'TrCCTGATGG

CGCAA'ITGAC

GTGGTACCCC

GAGATIAAC

GTTTAGATGG

CAAGA--ATCC

AAGTTTTAGC

GGGAAAGTGT

TGGGTCCTTI'

CAAGCCAATC

CGCAATCGAC

CGACCCCTAC

CAGCAACTAG

ATACAGCGAT

CTAAGGAATT

CGCCAGA.AGA

ATCGTGAAGA

GGACCCAAGC

TCACCGACGA

AAGTAACTTT

ATACAATGAC

ACATTGAACA

TGTTACATTC

ACGTAGAAGG

TCAAAACAC

TTAATAAAGT

CAGCTAACGG

CNGACAGCTA

AAGCTAGCGC

AAGCCGACTT

CACCACCT'GC

ATrTAGA~T

ACGAAACAAG

TCACAGACGT

CACAAGAAAA

TAGCTGGTCA

AAGAATTAGC

GCAACGALAGG

CAGA-AGACCC

ATTATCGACA

TTTAGCTGAA

GAAAGACGAC

TCAAGCGGGT

TTCAGATAAT

TCAACACCC

AAAGACAAAA

TTATGATAAA

AACCCCCCCA

GAATCGAAAA

TTACTTGCAT

TCTTCAAGTC

GTTAAACCAA

TGAATCTTTA

TGTAAAACAA

TAITGATGAT

ACCAGCAACA

GGACGATGCT

TACAGAAGGT

TGATGCAGCA

TCCAGAAGCG

TCCAACGATT

TAGTTTCGAT

CAGCATGGTG

AAATGGTAAA

TGAAATGAAC

TATCACCACT

AGGCGGGATT

CAACAAACCA

ACAAGAACAT

TTCGGTAAC

GTI'AGACATC

CAAAGTAACA

TGACTAITA

AACGGACGAA

GAACTTTGGC

ACCAACGCCA

AACCAACCGT

CACATGGACC

GAAAG'ITNCT

TAACAAAGTA

TACATACACA

GCcTTATATT

TGACGTGTTG

AAAAAAACCT

S

S

147 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

GAACCTAA.AC

ACGAACCCAG

ACAACAGTAA

TTTGGCATAA

AACCCCTAAA ACCGAAAAAA CCGTTGACGC CTACAA.ATCA TCAAGCACCA TCAATTTGG AAAATCAGCA AGTAAAGGAA TTCA=rACC AATGACTAAT ATCCACTFIA CATGATCGCA GGTTTAATTG TCCTI'ATAGT GGCTATTAGC CA.AAAAATAA AAAAAGAAAA AATTAG EF065-2 (SEQ ID NO:246) MF KKATKLLSTM VIVAGTVVGN FSPTLALAEE AVKAGDTEGM TN'IVKVKDDS

LADCKRILEG

MLASYRGGKQ

LKLALDTYNQ

VEYSUDYQGA

LQQGSSTPED

NDVPVQINGQ

IPKNDNAHAC

DINKVLDI ID

IKTDATDEEL

DLTNRDQEFK

ENNKVTFEMN

EGDVLHSDTKP

ASMVDDINKV

TITTKIKTDA

PKQPLKPKKP

GITKNKKRKN

QATFPVQAGE

FMFPDGKTK I

THGDLTNRKT

AAEVLALNQE

FITSQS IDDF

TISATSTEGY

DVTPEDPTIT

VYKVTDENGKD

APYIEQGGIP

WNVKTAFGNE

XQADSYDYLS

TVTPPAPTPE

LDITDVKVXX

TDEELAPYI E

LTPTNHQAPT

TEPVDLVVVE

NSADYDMNVR

YFLLVTDGVA

ITNQGYENiIN TTQLKQ IVKD

VGNITIHYEV

KD IENQEHLD

VTANGTVTQE

NQADLNFGNE

TSTWTQASMV

G14TYTMTITT

DPTITKDIEG

ENGKDVTDNG

QGGI FNQADL

NPVNFGKSAS

DASCSFSDNF

VNTQLTYDXS

NTRLDGYLEK

kYWESVESLS

RLAQSTPATA

KENTAIIDAAT

LT.NREDSFDW

NN~KVTFE-M

GDVLHSNXPT

DDINKVLDIT

K IKASATDEE

QEHLOLTNRD

IVTQENNRVTr

NFGNEGDVLH

KG IHLPMTNT

PHVRQAIDEV

QFVSGFCDVR

TNTNDSINEY

SVNSYFDKYK

SLTIANQFDI

LVSSGTMNQG

HVKTAFGNET

QADSYDYLSG

VTPPPVDPNI

DVKVTDENGK

LAPYIEQGG I

QEFKWNVKTA

FTtNKKDDSY

SNKPTVTPPA

TVNP LYM lAG

VQGLSDQDRV

TYGGTPTAPG

PDPRHPLQVS

TEVGPFVKQE

QSATATDDAG

TIAKEFPEAT

STTQASMVD

HTYTMTITTK

AKDVEGQEHL

DVTANGKVTQ

PNQADLNFGN

FGNETSTWTQ

SYLAGHTYTM

PTPEDPKKE

L IVLIVAI SF

S..

S.

S

S.

EF065-3 (SEQ ID NO:247) GGITA AAGCAGGAGA TACAGAAGGA ATGACCAATA CGGTGAAAG-T GAAAGACGAC

AGTCTGGCTG

GAAACGGAAC

TTTCCACATG

GTGATGCTGG

ATTAATTCAG

AGCCAATTTC

GGATTGAAAC

ACGTATTTCC

AAGACCAATA

TCAGTGGAAT

GAAATTACTA

AGTTCAGTG-A

GAGTT1GCAAC

TTTACAACCC

GCTTCATTAA

GGAAATGATG

TACGTAGGAA

ACCCI'GTAA

ACGATTCCTA

ACAAAAGATA

TGGCATGTCA

GATGACAFI'A

GATGTTACAG

AAACAAGCAG

AAAA71AAAA

ATTGTAAACG

CAGTCGATTT

TAAGACAAGC

CTTCATATCG

CTGATTATGA

TCTCTGGITT

TCGCTTTAGA

TATTAGTGAC

CCAATGAITC

ATAGTAATGA

ACCAAGGCTA

ATI'CATACTI

AAGGGTCTAG

AATTAAAACA

CGATTGCCAA

TGCCTGTTCA

ACATCACGAT

GTAGTGGGAC

AAAATGACAA

TCGAAAATCA

AAACAGCCT

ATAAAGTGCT

CTAACGGCAC

ACAGCTATGA

CTGACGCAAC

GATATTGGAA

AGTAGTTGTT

GATI'ATGAA

CGGCGGAAAA

TATGAATGTG

TGGAGACGTT

TACGTACAAT

AGATGGGGTC

AATCAATGAA

CTACCAAGGT

TGAAATCATT

WATAAATAT

CACACCAGAA

AATTGTCAAA

TCAATTTGAT

AATTAACGGA

TCACTACGAA

AATGAATCAA

TGCGCATGCG

AGAACACTTA

TGGCAACGAA

AGATATCATT

AGTAACACAA

CTATTTAAGT

GGACGAAGAA

GGACAAGCTA CTTTCCCAGT TCAAGCGGGT GAAGATGCTA GTGGTAG=I TTCAGATAAT GTGGTTCAAG GCTTATCTGA TCAAGACCGC CAAT'TATGT TTCCTGATGG AAAGACAAAA CGCGTCAATA CGCAATI'GAC TTATGATAAA CGGACGTATG GTGGTACGCC AACCGCCCCA CAAACACACG GAGATI'TAAC GAATCGAAAA GCTAATACAC GTITAGATGG TTACITGCAT TATCCAGATC CAAGACATCC TCTTCAAGTC GCAGCAGCAG A.AGTTI'AGC GTTAAACCAA AATGCGTATT GGGAAAGTGT TGAATCTITTA AAAACAGAAG TGGGTC TGTAAAACAA GAI=IATTA CAAGCCAATC TATTGATGAT GATCGTCTGG CGCAATCGAC ACCAGCAACA A'FrCAATCTG CGACCGCTAC GGACGATGCT CAAACCAT'T CAGCAACTAG TACAGAAGGT G-TCAAAGAAA ATACAGCGAT TGATG-CAGCA GGAACAATTG CTAAGGAT TCCAGAAGCG TGTGACGTGA CGCCAGAAGA TCCAACGATT GAT~rAACCA ATCGTCAAGA TAGTTTCGAT ACCAGTACTI GGACCCAkAGC CAGCATGGTG GATGTGAAAG TCACCGACGA AAATGGTAAA GAAAATAACA AAGTAACTTT TGAAATGAAC GGTCATACGT ATACAATGAC TATCACCACT TTAGCGCC'IT ACATGAACA AGGCGGGAT!' TABLE I1. Nucleotide and Amino Acid Seqeuences of E. faccalis Genes.

CCCAACCAAG

ACCGTAACAC

TTAGATTTAA

GAAkACAAGCA

ACTGATGTAA

CAAGAAAATA

ACTGGTCATA

GAATTAGCAC

AACGAAGGTG

GAAGATCCAA

GACCAAGAAT

CAAGCCAGCA

CANGAAAATG

AC I=TACTA

ATGACTATTA

GAACAAGGCG

CATTCCAACA

GAACCTAAAC

ACGAACCCAG

C--GACTTAAA

CACCGCCAGT

CCAACCGCGA

CTTGCACCCA

AAGTCACAGA

ACAAAGTAAC

CGTACACAAT

CTTATATTGA

ACGTTGCA

CGATTACAAA

TIAAATGGAA

TGGT'GGATGA

GCAAAGATGT

TGAACAAAAA

CCACTAAAAT

GOATrCCCAA

AGCCAACCGT

AACCGCTAAA

TCAATTTGG

C=~GGCAAT

TGATCCAAAT

TCAAGAATTT

AGCCACCATG

TGAAAATGGT

T'ITTGAAATG

CACCATTACT

ACAAGGTGGC

TTCCAACAAA

AGATATCGAA

CGTCAAAACA

CA'ITAATAAA

TACAGATAAT

AGATGACAGC

TAAAACTGAC

CCAAGCCGAC

AACACCGCCT

ACCGAAAAAA

AAAATCAGCA

GAAGGTGACG

AT*TGCTA-AAG

AAkATGGAACO

GTAGATGACA

AAAGAT'GTTA

AACAANCAAG

ACTAAALATCA

ATTCCCA.ACC

CCAACCGTAA

GGCCAAGAAC

GCTTTCGGTA

GT(.-TTAGACA

GGCATAGTAA

TACTCTTACT

GCAACGGATG

TTAAACTTI'TC

GCACCA.ACGC

CCGTTGACGC

AGTAAAGGAA

Tr.TTACATTC

ACGTAGAAGG

TCAAAACAGC

'rrAATAAAGT

CAGCTAACGG

CNGACAGCTA

AAGCTAGCGC

ALAGCCGACTT

CACCACCTGC

ATTTAGATTT

ACGAAACAAG

TCACAGACGT

CACAAGAAAA

TAGCTGTCA

AAGAATTAC

GCAACGAAGG

CAGAAGACCC

CTACAAATCA

TT

CAACAAkACCA

ACAAGAACAT

TTTCGGTAAC

GTTAGACATC

CAAAGTAACA

TGACTATTTA

AACGGACGAA

GAACTTTGGC

ACCAACGCCA

AAICCAACCCT

CACATGGACC

GAAAGTTNCT

TAACAAAGTA

TACATACACA

GCCTTATATT

TGACGTGTTG

AAAAAAACCT

TCAAGCACCA

EF065-4 (SEQ ID NO:248) a a a

AVKAGIJTEGM

£LADCKRILE G

MLASYRGGKQ

LKLALDTYNQ

VEYSNDYQGA

LQQGS STED

NDVPVQINGQ

IPKNONAHAC

D INXVLIDI I D

IKTDATDEEL

DLTNRDQEFK

ENNKVTFEM

EGDVLHSNKP

ASMVDDINKV

TITTKIKTDA

PKQPLKPKKP

TNTVKVKDDS

QATFPVQAGE

FMFPDGKTKI

THGDLTNRKT

AAEVLALNQE

FITSQS IDDF TI SATSTEGY

DVTPEDPTIT

VKVTDENGKD

APYIEQGGIP

WNVKTAFGNE

XQADSYDYLS

TVTPPAPTPE

LDITDVKVXX

TDEELAPYIE

LTPTNHQAPT

TEPVDLVVVE

NSADYDMNVR

YFLLVTDGVA

ITNQGYEMIN

'ITQLKQIVKD

VGNITIHYEV

KDIENQEHLD

VTANGTVTQE

NQADLJNFGNE

TSTWTQASMV

GHTYTMTITT

DPTITKDIEG

ENGKDVTDNG

QGG IPNQADL

NPVNF'GKSAS

DASGSFSIDNF

VNTQLTYDKS

NTRLDGYLHK

AYWESVESLS

RLAQSTPATA

KENTAIDAAT

LTNREDSFDW

NNKVTFEIM

GDVLHSN'KPT

DDINKVLDIT

K IKASATDEE

QEHLDLTNRD

IVTQEiNNKVT

NFGNEGDVLH

KGIH

PHVRQAIDEV

QFVSGFCGDVR

TNTNDSINEY

SVNSYFOKYK

SLTIANQFDI

LVSSGTMNQG

HVKTAFONET

QADSYDYLSG

VTPPPVrEPNI

DVKVTDENGK

LAPY IEQGGI

QEFKWNVKTA

FTMNKKDDSY

SNKPTVTPPA

VQGLSDQDRV

TYGGTPTAPG

POPRHPLQVS

TEVGPFVKQE

QSATATDDAG

TIAKEFP EAT

STWTQASMVD

HTYTMTITTK

AKDVEGQEHL

DVTANGKVTQ

PNQADLNFGN

FGNETSTWTQ

SYLAGHTYTM

PTPEDPKKPE

EF066-1 (SEQ ID NO:249)

TAGCGAAACA

ATGGTGATTG

GAAGCGGTTA

AGTCTGCCTG

GAAACGGAAC

TTCCACATG

GTGATGCTGO

ATTAAT-TCAG

AGCCAATTTG

GCATI'GAAAC

ACGTATTTcC

AAGACCAATA

TCAGTGGAAT

AAATAGGGAG

'TCGCTGGAAC

AAGCAGGAGA

ATTGTAAACC

CAGTCGATTT

TAAGACAAGC

CTTCATATCG

CTGATTATGA

TCTCTGG I=

TCGCTTTAGA

TATTAGTGAC

CCAATGATI'C

ATAGTAATGA

GATTAAAATG

AC ITGTGGGA

TACAGAAGGA

GATATTGGAA

AGTAGTTGTT

GATI'GATGAA

CGGCGGAAAA

TATGAATGTG

TGGAGACGTT

TACGTACAAT

AGATGGGTC

AATCAATCAA

CTACCAAGGT

TTI'AAGA.AAG

AATI-TCACTC

ATGACCAATA

GGACAAGCTA

GAAGATGCTA

GTGG'ITCAAG

CAATTTATGT

CGCGTCA.ATA

CGGACGTATG

CAAACACACG

GCTAATACAC

TATCCAGATC

GCAGCAGCAG

CAACGAAATT

CCACATICC

CGGTGAAAGT

C'I=CCCAGT

GTGCTATI'

GCTTATICTGA

TTCCTGATGG

CGCAATTGAC

GTGGTACCC

GAGATTTAAC

G=TAATGG

CAAGACATCC

AAGTTITAGC

ATTATCGACA

TTTAGCTGAA

GAAAGACG-AC

TCAAGCGGGT

TTCAGATAAT

TCAAGACCGC

AAAGACAAAA

TTATGATAAA

AACCGCCCCA

GAATCGAAAA

TTACTTGCAT

TCTTCAAGTC

GTTAAACCAA

149 TABLE 1. Nucleotide and Amnino Acid Seqeuences of E.faecalis Genes.

GAAAITACTA

AGTTCAGrA

GAGTTGCAAC

TIrAC.AACCC

GCTTCATTAA

GGAAATGATG

TACGTAGGAA

ACCCTTGTAA

ACGATTCCTA

ACAAAAGATA

TGGCATGTCA

GATGACA ITA CATGT'rACAG

AAACAAGCAG

AAAATTAAAA

CCCAACCAAG

ACC.GTAACAC

TTAGATTTAA

GAAACAAGCA

ACTGATGTAA

CAAGAAAATA

AGTGGTCATA

GAATTAGCA.C

AACGAAGGTG

GAAGATCCAA

CACCAAGAAT

CAAGCCACCA

GANGAAAATG

AC=TTACTA

ATGACTATTA

GAACAAGGCG

CATTCCAACA

GAACCTAAAC

ACGAACCCAG

ACAACAGTAA

TT~rCCCATAA

ACCAAGGCTA

ATTCATACT

AAGGGTCTAG

AATTAAAACA

CGATTGCCAA

TCCCTCTTCA

ACATCACCAT

GTAGTGCGAC

AAAATGACAA

TCGAAAATCA

AAACAGCCTT

ATAAAGTGCT

CTAACGGCAC

ACAGCTATGA

CTGACGCAAC

CCGACTTAAA

CACCGCCAGT

CCAACCGCGA

CTTGGACCCA

AAGTCACAGA

ACAAkAGTAAC

CGTACACAAT

CTTATATTGA

ACGTGTTGCA

CGATTACAAA

TTAAATGGAA

TGGTGGATGA

GCAAAGATGT

TGAACAAAAA

CCACTAAAAT

GGATTCCCAA

AGCCAABCCGT

AACCGCTAAA

TCAArTCG

ATCCACTTTA

CAAAAAATAA

TCAAATGCATT

TGATAAATAT

CACACCAGAA

AATTCTCAAA

TCAATTTGAT

AATTAACGGA

TCACTACGAA

AATGAATCAA

TGCGCATGCG

AGAACACTTA

TGGCAACCAA

AGATATCATI'

AGTAACACAA

CTATTTAACT

GGACGAkAGAA

CTITTGCAAT

TGATCCAAAT

TCAAGAkATT

AGCCAGCATG

TGAAAATCGT

TTTTGAAATC

GACCATTACT

ACAAGGTGGC

TTCCAACAAA

AGATATCGAA

CGTCAAAACA

CATTrAATAAA

TACAGATAAT

AGATGACAGC

TAAAACTCAC

CCAAGCCGAC

AACACCGCCT

ACCCAAAAAA

AAAATCAGCA

CATCATCGCA

AAAAAGAAAA

AATGCGTATr GGGAAAGTG3T TGAATCTFTA AAAACAGAAG TGGGTCCTTT TGTAAAACAA GATTTTATIA CAAGCCAATC TATTGATGAT GATCGTCTCG CGCAATCGAC ACCAGCAACA ATTCAATCTG CGACCCCTAC CGACGATC-CT CAAACCATTT CAGCAACTAG TACAGAAGGT GTCAAAGAAA ATACAGCGAT TGATGCAGCA GGAACAATTC CTAAGCAATT TCCAGAAGCC TGTGACGTGA CCCCAGAAGA TCCAACGATT GATTTAACCA ATCGTGAAGA TAGTTTCGAT ACCAGTACTT GGACCCAAGC CAGCATCGTG GA'±GTGAAAG TC:ACCC;ACGA AAATGGTAA.A GAAkAATAACA AAGTAACTTT TGAAATGAAC GGTCATACCT ATACAATGAC TATCACCACT TTAGCGCCTT ACATTGAACA AGGCGGGATT GAAGGTGACG TGTTACATI'C CAACAAACCA ATTGCTAAAG ACGTAGAAGG ACAAGAACAT AAATGGAACrG TCAAAACAGC TTTCGGTAAC GTAGATGACA TTAATAAAGT GTTAGACATC AAAGATTA CAGCTAACGG CAAAGTAACA AACAANCAAG CNGACAGCTA TCACTATTA ACTAAAATCA AAGCTAGCGC kACGGACGAA ATTCCCAACC AAGCCGACTT GAACTTTGGC CCAACCGTAA CACCACCTGC ACCAACGCCA GGCCAAGAAC ATTTAGATTT AACCAACCGT GCT CGGTA ACGAAACAAG CACATGGACC GTCTTAGACA TCACAGACGT GAAAGTTNCT GGCATAGTAA CACAAGAAAA TAACAAAGTA TACTCTTACT TAGCTGG'TCA TACATACACA GCAACGGATr. AAGA.ATTAGC CCCTTATATT TTAAACTTTG GCAA-CCAAGG TGACGTGTTG GCACCAACGC CAGAAGACCC AAAAAAACCT CCGTTGACGC CTACAAATCA TCAAGCACCA AGTAAAGGAA TrCA=LACC AATGACTAAT GG=TAA=F TCCTTATAGT GGCTATTAGC

AATTAG

o 00.

EF066-2 (SEQ ID NO:250) MF KKATKLLSTM VIVACTVVGN FSPTLALAEE AVKAGDTEGM TNIVKVKDDS 0 0000 00 0 ~0 00

LADCKRIELEG

MLASYRGGKQ

LKLALDTYNQ

VEYSNDYQGA

LQQGSSTPED

NDVPVQ INGQ

IPKNDNAHAC

DINXVLDI ID

IKTDATDEEL

DLTNRDQEFK

ENNKVTFEM

EGDVLHSNKP

ASMVDD INK V TITTK IKTDA

QATFPVQAGE

FMFPDGKTKI

THGDLTNRKT

AAEVLAIJNQE

FITSQS IDDF

TISATSTEGY

DVTPEDPTIT

VKVTDENGKD

APYIEQGGIP

WNVKTAFGNE

XQADSYDYLS

TVTPPAPTPE

LDITDVKVXX

TDEELAPYIE

TEPVDLVVVE

NSADYDMNVR

YFLLVTDCVA

ITNQGYEMIN

TI'QLKQIVYKD

VGNITIHYEV

KDIENQEHLD

VTANGTVTQE

NQADLNFGNE

TSTWTQASMV

GHTYTMT ITT

DPTITKDIEG

ENGKDVTDNG

QGGI PNQADL

DASGSFSDMF

VNTQLTYDKS

NTRLDGYLHK

AYWESVESLS

RLAQSTPATA

KENTAI DAAT

LTURE-DSFDW

NKVTFEMNK

GDVLI4SNKPT

DDINKVLDIT

KIKASATDEE

QEHLDIJTNRD

IVTQENNKVT

NFGNEGDVLH

PHVRQAIDEV

QFVSGFGDVR

TNTNDSINEY

SVNSYFDKYK

SLTIANQFDI

LVSSGTMQG

HVKTAFGNET

QADSYDYLSG

VTPP PVDPNI

DVKVT-DENGK

LAPY IEQGG I

QEFKW.NVKTA

FTMNKKODSY

SNKP'IVTPPA

VQGLSDQDRV

TYGGTPTAPG

PDPRHPLQVS

TEVGPFVKQE

QSATATDDAG

TIAKEFPEAT

STWTQASMVD

HTYTMTITI

AKDVEGQEH-L

DVTANCKVTQ

PNQADLNFGN

FGNETSITQ

SYLACHTYTM

PTPEDpKKPE 150 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

PKQPLKPKKP LTPTNIHQAPT NPVNFGKSAS KGIHLPMTNT TVNPLYMIAG LIVL7VAISF C ITKNKKRKN EF066-3 (SEQ ID NO:251) GGTTA AAGCAGGAGA TACAGA-AGGA ATGACCAATA CGGTGAAAGT GAAAGACGAC

AGTCTGGCTG

GAAACGGAAC

TTTCCACATG

CTGATGCTGG

ATTAATTCAG

AGCCAArI'TG

GGATTGAAAC

ACGTATTTCC

AAGACCAATA

TCAGTGGAAT

GAAATTACTA

AGTTCAGTGA

GAGTTGCAAC

TTTACAACCC

GCTI'CATTAA

GGAAATGATG

TACGTAGGAA

ACCCTICTAA

ACGATTCCTA

ACAAA.AGATA

TGGCATGTCA

GATGACATTA

ATTGTAAACG

CACTCGATTT

TAAGACAAGC

CTTCATATCG

CTCATTATGA

TCTCTGGTTT

TCGCTTTAGA

TATTAGTGAC

CCAATGATTC

ATAGTAATGA

ACCAAGGCTA

ATI'CATACTT

AAGGGTCTAG

AATTAAAACA

CGATTGCCAA

TGCCTGTTCA

ACATCACGAT

GTAGTCGGAC

AAAATGACAA

TCGAAAATCA

AAACAGCCTT

ATAAAGTCCT

GATA'IGAA

AGTAGTTCTT

GATTGATGAA

CGGCGGAAAA

TATCAATCTG

TGGAGACGTT

TACGTACAAT

AGATCGGGTC

AATCAATGAA

CTACCAAGGT

TGAAATGA'T

TGATAAATAT

CACACCAGAA

AATTGTCAAA

TCAATTTGAT

AATTAACGGA

TCACTACGAA

AATGAATCAA

TCCATGCG

AGAACACTI'A

TGGCAACGAA

AGATATCATT

GGACAAGCTA

GAACATGCTA

GTGGTTCAAG

CAATTTATLGT

CGCGTCA-ATA

CGGACGTATG

CAAACACACG

GCTAATACAC

TATCCAGATC-

GCAGCAGCAG

AATGCGTAIT

AAAACAGAAG

GATTITATTA

GATCG;TCTGbG

ATTCAATCTC

CAAACCATT

GTCAALAGAAA

GGAACAATTG

TGTGACGTGA

GATTTAACCA

ACCAGTAC'T

GATGTGAAAG

CTTTCCCAGT

GTCGTAG'FIT

GCTTATCTGA

'PTCCTGATGG

CGCAATTGAC

GTGGTACGCC

GAGATTAA

GTI'TAGATGG

CAAGACATCC

AAGT=IAGC

GGGAAAGTGT

TCCGTCC=T

CAAGCCAATC

CGCA.ATCGAC

CGACCGCTAC

CAGCAACTAG

ATACAGCGAT

CTAAGGAATT

CGCCAGAAGA

ATCGTGAAGA

GGACCCAAGC

TCA

TCAAGCGGGT

TTCACATAAT

TCAAGACCGC

AA.AGACAAAA

TTATGATAAA

AACCGCCCCA

GAATCGAAAA

TTACTTGCAT

TCTTCAAGTC

GTTAAACCAA

TGAATCTTTA

TGTAAAACAA

TATTGATGAT

ACCAGCAACA

GGACGATGCT

TACAGAAGGT

TGATGCAGCA

TCCAGA-AGCG

TCCAACGATT

TAGT'=CGAT

CAGCATGGTG

S.

SS

S

S

S

55 S S S 55 55 5

S

EF066-4 (SEQ ID.NO:.252) AVKAGD'rEGM TNTVKVKDDS

LADCKRILEG

MLASYRGGKQ

LKLALDTYNQ

VEYSNDYQGA

LQQGSSTPED

NDVPVQ INGQ

IPKNDNAHAC

QATFPVQAGE

FMFPDGKTK I

THGDLTNRKT

AAEVLALNQE

FITSQSIDDF

TI SATSTEGY

DVTPEDPTIT

TEPVDLVVVE

NSADYDMNVR

YFLLVTDGVA

ITNQGYEMIN

TTQLKQ IVKD

VGNITIHYEV

KDIENQEHLD

DASGSFSDNF

VNTQLTYDKS

NTRLDGYLHK

AYWESVESLS

RLAQSTPATA

KENTAIDAAT

LTNREDSFDW

PHVRQAIDEV

QFVSGFGDVR

TNTNDS INEY

SVNSYFDKYK

SLTIANQFDI

LVSSGTMNQG

1-VXTAFGNET

VQGLSDQDRV

TYGGTPTAPG

PDPRHPLQVS

TEVGPFVKQE

QSATATDDAG

TIAKEFPEAT

STWTQASMVD

DINKVLDIID VKVT EF067-1 (SEQ ID NO:253)

S

S. S

S.

*5

TAGCGAAAGA

ATGGTGATTG

GAAGCGGTTA

AGTCTGGCTrC

GAAACGGAAC

T TCCACATC.

GTGATGCTGG

ATTAATTCAG

AGCCAATTTG

GGATTGAAAC

ACGTATI'TCC

AAATAGGGAC

TCGCTGGAAC

AAGCAGGAGA

ATI'GTAAACG

CAGTCGATTT

TAAGACAAGC

CTTCATATCG

CTGAITATGA

T CTCTGGT

TCGCTTTAGA

TATTAGTGAC

GA'ITAAAATG

AGTI'GTGGGA

TACAGAAGGA

GATATTCGAA

AGTAGTTGTT

GATTGATGAA

CGGCGGAAAA

TATGAATIGTG

TGGAGACG T

TACGTACAAT

AGATGGGGTC

T ETAAGAAAG

AATTTCAGTC

ATGACCAATA

GGACAAGCTA

GAAGATGCTA

GTGG TCAAG CAA IITATCT

CGCGTCA-ATA

CGGACGTATG

CAAACACACG

GCTAATACAC

CAACGAAAT

CCACAMIGC

CGGTGAAAGT

CTTTCCCAGT

GTGGTAGTTr

GCTTATCTGA

'IWCCTCATGG

CGCAA'ITAC

GTGGTACGCC

CGATTTAAC

GTTTAGATGC

ATTATCGACA

TTTAGcTGAA

GAAAGACGAC

TCAAGCGGGT

ITrcAGATAAT

TC-AAGACCGC

AAAGACAAAA

TTATGATAALA

AACCGC CC CA

GAATCGAAAA

ITACTTGCAT

151 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.f[aecalis Genes.

AAGACCAATA

TCAGTGGAAT

GAAA'ITACTA

AGTITCAGTGA

GAGTTGCAAC

TTTACAACCC

GCTTCATTAA

GGAAATGATG

TACGTAGGA-A

ACCCTTGTAA

ACGATTCCTA

ACAAAAGATA

TGGCATGTCA

GATGACATI'A

GATG TTACAG

AAACAAGCAG

AAAkITTAAAA

CCCAACCA.AG

ACCGTAACAC

TTAGATTAA

GC.AACAAGCA

ACTGATGTAA

CAAGAAAATA

AGTGGTCATA

GAATTAGCAC

AACGAAGGTG

GAAGATCCAA

GACCAAGAAT

CAAGCCAGCA

GANGAAAATG

ACTTTACTA

ATGACTATrA

GAACAAGGCG

CATTCCAACA

GAACCTAAAC

ACGAACCCAG

ACAACAGTAA

rI'TGGCATAA

CCAATGATTC

ATAGTAATGA

ACCAAGGCTA

ATrCATACT

AAGGGTCTAG

AATTAAAACA

CGATTGCCAA

TGCC-TGTTCA

ACATCACGAT

GTAGTGGGAC

AAAATGACAA

TCGAAAATCA

PAACAGCCTT

ATAAAGTGCT

CTAACCGCAC

ACAGCTATGA

CTGACGCAAC

CCGACTTAAA

CACCGCCAGT

CCAACCGCGA

CTI'GGACCCA

AAGTCACAGA

ACAAAGTA.AC

CGTACACAAT

CTTATATTGA

ACGTGrI'GCA

CGATTACAAA

TTAAATGGAA

TGGTGGATGA

GCAAAGATGT

TGAACAAAAA

CCACTAAA.AT

GGATTCCCAA

AGCCAACCCT

AACCGCTAAA

TCAATrTGG

ATCCACTTTA

CAAAAA.ATAA

AATCAATGAA

CTACCAAGGT

TGAAATGATT

TGATAAATAT

CACACCAGA-A

AATTGTCAAA

TCAATrAT

AATTAACGGA

TCACTACGAA

AATGAATCAA

TGCGCATIGCG

AGAACACTTA

TIGGCAACGAA

AGATATCATT

AGTAACACAA

CTATITAAGT

GGACGAAGAA

CTTTGGCAAT

TGATCCAAAT

TCAAGAATTT

AGCCAGCATG

TGAAAATGGT

TTTTGAAATG

GACCATTACT

ACAAGGTGGC

TTCCAACAAA

AGATATCGAA

CGTCAA.ACA

CATTAATAAA

TACAGATAAT

AGATGACAGC

TAAAACTGAC

CCAAGCCCAC

AACACCGCCT

ACCGAAAAAA

AAAATCAGCA

CATGATCGCA

AAAAAGAAAA

TATCCAGATC

GCAGCAGCAG

AATGCGTAI'

AAAACAGAAG

GATTTATI'A

GATCGTCTGG

ATTCAATCTG

CAAACCAFTr

GTCAAAGAAA

GGAACA.ATG

TGTGACGTGA

GATTTAACCA

ACCAGTACI'

GATGTG-AAAG

GAAAATAACA

GGTCATACGT

TTAGCGCC!IT

GAAGGTGACG

ATTGCTAAAG

AAATGGAACG

GTAGATCACA

AAAGATGTrA

AACAANCAAG

ACTAAAATCA

ATTCCCAACC

CCAACCGTAA

GGCCAAGAAC

GCTTTCGCTA

GTGTTAGACA

GGCATAGTAA

TACTCTTACT

GCAACGGATG

TT-AAACT'Fr

GCACCAACGC

CCGTTGACC

AGTAAAGGAA

GGTAATTG

AATTAG

CAAGACATCC

AAG'=''AGC

GGGAAAGTGT

TGGGTCCTTT

CAAGCCAATC

CCCAATCGAC

CGACCGCTAC

CAGCAACTAG

ATACAGCGAT

CTAAGGAATT'

CGCCAGAAGA

ATCGTGAAGA

GGACCCAAGC

TCACC&ACGA

AAGTAACTTT

ATACAATGAC

ACATTGAACA

TGTTACATTC

ACGTAGAAGG

TCAAAACAGC

TTAATAA.AGT

CAGCTAACGG

CNGACAGCTA

ALAGCTAGCGC

ACCGrACTT

CACCACCTGC

ATTTAGATT

ACGAAACAAG

TCACAGACGT

CACAAGAAAA

TAGCTGGTCA

AAGAAT-LAGC

GCAACGAAGG

CAGAAGACCC

CTACAAATCA

TTCA~rACC

TCCTTATAGT

TCTTCAAGTC

'TTAAACCAA

TCAATCTTTA

TGTAAAACAA

TATTGATGAT

ACCAGCAACA

GGACGATGCT

TACAGAAGGT

TGATGCAGCA

ICCAGAAGCG

TCCAACGATT

TAGTTTCGAT

CAGCATGGTG

AAATGGTAAA

TGAAATGAAC

TATCACCACT

AGGCGGGATT

CAACAAACCA

ACAAGAACAT

TTTCGGTAAC

GTTAGACATC

CAAAGTAACA

TGACTATTTA

AACGGACGAA

GAACTI'TGGC

ACCAACGCCA

AACCAACCGT

CACATGGACC

GAAAGTTNCT

TAACAAAGTA

TACATACACA

GCCTTATATT

TGACGTGTTG

AAAAAAACCT

TCAAGCACCA

AATGACTAA-t

GGCTATTAGC

.0* S S

S.

S.

S S

S.

S.

S S

S

S.

S *5*S

S

S

*5 5O

S.

EF067-2 (SEQ ID NO:254) HF KKATKLLSTM VIVAGTVVGN FSPTLALAEE AVKACDTEGM TNTVKVKDDS LADCKRI LEG

MLASYRGGKQ

LKLALDTYNQ

VEYSNDYQGA

LQQGSSTPED

NDVPVQINGQ

IPKNDNAHAC

DINKVLDIID

IKTDATDEEL

DLTNRDQEFK

E~NKVTFEM

EGDVLHSNKP

QATFPVQAGE

FMFPDGKTKI

THGDLTNRKT

AAEVLALNQE

FITSQSIDDF

TISATSTEGY

DVTPEDPTIT

VKVTDENGKD

APYIEQGGIP

WNVKTA.FGNE

XQADSYDYLS

TVTPPAPTPE

TEPVDLVVVE

NSADYDMNVR

YFLLVTDGVA

ITNQGYEMIN

TTQLKQIVKD

VGNITIHYEV

KDIENQEHLD

VTANGTVTQE

NQADLNFGNE

TSTWTQASMV

GHTYTMTITT

DPTITKDIEG

DASGSFSDNF

VNTQLTYDKS

NTRLDGYLHK

AYWESVESLS

RLAQSTPATA

KENTAIDIAAT

LTNREDSFOW

NNKVTFEI4NK

GDVLHSNKPT

DDINKVLDIT

KIKASATDEE

QEHLDLTI'RD

PHVRQAIDEV

QFVSGFGDVR

TNTNDS INEY

SVNSYFDKYK

SLTIANQPDI

LVSSGTMNQG

HVKTAFGNET

QADSYDYLSG

VTPPPVDPNI

DVKVTDENGK

LAPYIEQGGI

QEFKVINVKTA

VQGLSDQDRV

TYGGTPTAPG

PDPRHPLQVS

TrEVGPFVK<QE

QSATATDDAG

TIAKEFPEAT

STW.'TQASMVD

HTYTMTITTK

AKDVEGQEHL

DVTANGKVTQ

PNQADLNFGN

FGN"ETSTWTQ

152 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

ASMVDDINKV LDITDVKVXX ENGKDVTDNG IVTQENNKVT FTMXDDSY TITTXIKTDA TDEELAPYIE QGGIPNQADL NFGNEGDVLH SNKPTVT-PPA PKQPLKPKKP LTPTNHQAPT NPVNFGKSAS KGIHLPMTNT TVNPLYbIIAG G ITKNKKRKN

SYLAGHTYTM

PTPEDPKKPE

LIVLIVAISF

EF067-3 (SEQ ID NO:255) GCT AGATATCATT GATGTGAAAG TCACCGACGA AAATGGTAAA

GATO.TTACAG

AAACAAGCAG

AAAATTAAAA

CCCAACCAAG

ACCGTAACAC

TTAGATTTAA

GAAACAAGCA

ACTGATGTAA

CAAGAAAATA

AGTGGTCATA

GAATTAGCAC

AACGAAGGTG

GAAGATCCAA

GACCAAGAAT

CAAGCCAGCA

GANGAAAAT G

ACTTTTACTA

ATCACTATTA

GAACAAGGCG

CATTCCAACA

GAACCTAAAC

ACGA.ACCCAG

CTAACGGCAC

ACAGCTATGA

CTGACGCA.AC

CCGACTTAAA

CACCGCCAGT

CCAACCGCGA

CTTGGACCCA

AAGTCACAGA

ACAA.AGTAAC

CGTACACAAT

CTTATATTGA

ACGTGTTGCA

CGATTACAAA

TTAAATGGAA

TGGTGGATGA

GCAAAGATGT

TGAACAAAAA

CCACTAAAAT

GGA'ITCCCAA

AGCCAACCGT

AACCGCTAAA

TCAATTTTGG

AGTAACACAA

CTATTTAAGT

GGACGAAGAA

CTTTGGCAAT

TGATCCAAAT

TCAAGAATTT

AGCCAGCATG

TGAAAATGGT

TITTGAAATG

GACCATTACT

ACAAGGTGGC

TTCCA.ACAAA

AGATATCGAA

CGTCAAAACA

CA ITAATAAA

TACAGATAAT

AGATGACAGC

TAAAACTGAC

CCAAGCCGAC

AACACCGCCT

ACCGAAAAAA

AAAATCAGCA

GAAAATAACA

GGTCATACGT

7TAGCGCCTT

GAAGGTGACG

ATI'GCTAAAG

AAATOGAACG

GTAGATGACA

AAAGATGTTA

AACAANCAAG

ACTAAAATCA

ATTCCCAACC

CCAACCGTAA

GGCCAAGA-AC

GCTTICGGTA

6TGTTAGACA

GGCATAGTAA

TACTCTTACT

GCAACGGATG

TTAAACTTG

GCACCAACC

CCGTTIGACGC

AGTAAAGGAA

AAGTAACTTT

ATACAATGAC

ACATTGAkACA

TGTTACAFI'C

ACGTAGAAGG

TCAAAACALGC

FI'AATA.AAGT

CAGCTAACGG

CNGACAGCTA

AAGCTAGCGC

AAGCCGACTI'

CACCACCTrGC

ATTTAGATT

ACGAAACAAG

TCACAGACGT

CACAAGAAAA

TAGCTGGTCA

AAGAATTAGC

GCAACCAAGG

CAGA-AGACCC

CTACAAATCA

TT

TGAA.ATGAAC

TATCACCACT

AGGCGGGATT

CAACAAACCA

ACAAGA.ACAT

TTWTCGGTAAC

GTTAGACATC

CAAAGTAACA

TGACTATIA

AACGGACGAA

GAACT'ITGGC

ACCAACGCCA

AACCAACCGT

CACATGGACC

GAAAGTTNCT

TAACAAAGTA

TACATACACA

GCCTI'ATATT

TGACGTGFI'G

AAAAAAACCT

TCAAGCACCA

0@ 0@ 0 0* 00 0 0 *0 00 00 0 00 So 00 0 000 S 00 3 0 000 S 0 @00000 0 0000 0 0000 00 0000 0000 0 0000 000@ 0 0000 00 0 00 00 EF067-4 (SEQ ID NO:256) VLDIID VKVTDENGKD VTANGTVTQE NNXVTFEMUK QADSYDYLSG HTYTMTITTK

IKTDATDEEL

DLTNRDQEFK

ENNKVTFEN

EGDVLHSNKP

ASMVDDINKV

TITTKIKTDA

PKQPLKPKKP

APYIEQGGIP

WNVKTAFCNE

XQADSYDYLS

TVTPPAPTPE

LDITDVKVXX

TDEEL.APY IE

LTPTNHQAPT

NQADLNFGNE

TSTWTQASMV

GHTYTMTITT

DPTITKDIEG

ENGKDVTDNG

QGGIPNQADL

NPVNFGKSAS

GDVLHSNKPT

DDINKVLDIT

K IKASATDEE

QEHLDLTNRD

IVTQENNKVT

NFGNEGDVLH

KGIH

VTPPPVDPNI

DVzKVTDENGK LAPY IEQGG I

QEFKWNVKTA

FTNXKDDSY

SNKPTVTPPA

AKDVEGQEHL

DVTANGKVTQ

PNQADLNFGN

FGNETSTWTQ

SYLAAGHTYTM

PTPEDPKKPE

EF068-1 (SEQ ID NO:257)

TAGGGGAAGC

ATGAAA.AAGA

CGCAACATGC

GTAAGTATAG

CTATTATCAA

CCGCCAAACC

TCCGCTGTGT

AATGTAGCTG

TAATGATC T AAArITGTTGA

TTAATTTAGC

CTGTTACCTC

ATGTTACGTC

AAAATCAACC

TI'AGTGGACA

CAGCAGGCAG

GGTATTTATC

GGAFI'TTAAT

AATATCAACT

TGGCACAATC

AAATAATGAC

AGTTAATTTC

AAAACAAGCG

CGCAGCAATC

G=TTTTA

CGGAAAAGTC

GFIATTAT

AGTrtCATCAG

AGTOGGCACTT

ACGG=rCTG

GTGTTAGTGG

AATACCAATG

AAGAAAAGAG

AGCATAAAAA

TI'ACCTCAT

CAGCGGTC'T

CAACGAGTAA

GTGGCGCTFI'

'TCCTCCTGA

TCACGATTCGA

GGACGATCAG

ATGGACAAAA

AGCAATCCCT

GGATATCGAA

TCGTTGGACA

AGCAGATGCT

GTTAAGAGGA

TC'FTTCAAAA

153 TABLE 1. Nucleotide and Amino Acid Seqeucnces of E.[aecalis Genes.

GTTACTTTTT

ATTACCAGTG

CGTCAATTGG

GAAACGTTAG

GTTTTAGCCC

GAGGCAAAAG

CTTCCTGTCA

GGTGGTTCAG

TACCAACTA

GGAACAGTCG

TCCAACATTT

GTAACAGGTA

G=TGAAATCC

GCGTTTACAG

GCGAAAAACG

CCAACCGTAA

GTTAAAGGAA

ATAGGCACAG

GCAGGTGCCA

CCAACAACGT

GTGACAGGTA

GTTGAGATCC

GCATTTACAG

GCGAAAAACG

AATACGCCCG

GAGGTCAAAG

GTCCTTGGTA

ACAGCAACAG

CAACCAGCAA

GGCAACTCTG

GTTCGTGATC

ACTGTAACGC

GATAACGCGG

ACAGCACCAA

ACCGCTCATC

GGGACTGCCG

AATGAAACAT

TTCCAAACAC

ACAACACAAG

GCAACAGACG

ACGITAGC TT

ACTGGACTTG

ATTGG-TGACA

AATACCACCA

GATCAAGGAA

ACAGTTGTTG

GAAGATGCA

TATCAAGTAA

TTAGTTATTG

GGCACAGCAA

AGTAATCCGA

GTTGACAAAA

GGTACAACAG

ACTGATGGCA

TGACTGCCGT

GGGCGTTAGG

A.ATTAGTI'AA

CAGCTGACGG

AA.AATGTTTC

GTACCAGTAT

AAGGCACGGT

GCGTAAATGA

CCGTTTCAAC

TTCAAACAGA

ATTTTGCTGC

ATTCAACAGC

GAAATGCAGG

TTACCGTTCC

CCAGCGGNAC

CCGCACCAAC

CTCCCATGC

GTACCGCTGA

ATGAAACGTT

TCCAA.ACACC

ATTCAACAGC

GAAATGCAGG

TTACCATTCC

CCAGCGGTAC

TGGCGACGCC

GGACTCGCTGA

CT'GCAACAAC

CAAATCAAAC

CGCCGACAAC

GTTCGGGITA

CATCTGGGGC

TACCAACGGG

GAAATrGAAAG

CTGTAACAGG

CGAATGCTAC

ATGGGACTGC

TGACAGCGTI'

CAGCAGATGA

GATATCAAGT

GAACAGTTT

CAGGAAAAGC

AGAGTCA.ACC

TTACTGGAGA

TTGAAGTACG

ACTTTACTGT

GAAAAGACGG

CCGTAGCAGC

CCGGCACGGC

CTACGGGAAC

CAGCTAACGA

CTGCTTTCAA

TCACTGGTAG

TTGAGGTGCG

AATACACAGI

TTTGAATGCA

GAATTTAACT

TAACATTGAA

CTCATACATT

AAACATCTTA

CCCAAGTAAT

AAACGTGGCT

GTTAGTGGAT

ACCTCAAAAT

TCTTTAGAC

ACGCACTACT

AGGTI'ACGAA

AGGCACCGTA

CGCAGGTGAA

AGAAAGNACG

AATCACAGGA

CAATGCCACG

TGGGACAGGA

AACCGCCGTA

AGCGGATGAA

AGGITACGAA

AGGTGC'CGTG

CGCAGGTG;A

AGAAAGTACG

AATTGTTGAG

AGTIGGCACC

TGGAACTGAC

GCTGAGCGTT

ACCAGCTGAT

TCAAATTACA

AGTCATTGGT

AACGACCAAT

TCAACCGACT

AGTAACAGGT

CATCGAAATT

TI'CCTTTGCT

AGCCAAAGAT

AGTAGTGGCA

GACAGGTACC

AGGCACCGCA

AACAGCTAAA

AACTACAGCT

TTCAACAACT

GAACCCAGAT

GGACCTTCCA

TGACGGCAAT

ACCAACTGTG

AGAGCCAAAT

GAC.TGATGGT

AGCCTTAAC7

.AACACCTGCI

CACGACAAAC

TGACGCCGAI

GACT'IAGAG

GCCAATGAIT

GGTGTTGATA

AACTTAGGTG

AGTGCACCCA

CAAGATTTGA

CTTGTCGCCG

GTTCAGGTG

GCTTCTTTAC

TTATCCAATA

G'rrAA ITAT

AGTGAAGTAA

GTTAAAGGAA

ATAGGCACAG

GCAGGCGCCA

CCAACAACGT

GTGACAGGTA

GTTGAAATCC

GCGTTTACAG

GCGAAAAACG

GCAACCGTAA

GTTAAAGGAA

ATAGGTACAG

CCAGGTCA

CCAACAACCT

ACTGTAACAG

ACCATTGAGG

GAAATATA

GTAGCGAAAA

GTCACTGCAC

GGAACAGCAG

ACAGGTACCT

CCTGGGGATA

GAAGTCCTTG

AATTCAGTI'

CGTGATGCAG

GTGAACCTTC

CCTGCTGGCA

CCGCCAAGTG

GCTGAACTTG

ACAACTGGAC

CAAACAGTGA

ATGACACCCG

GGTTATGAAA

GGAACAATTA

GCGGGAGCCG

GAAAGTCAAC

ACGACTGTrA

GTCACCATTG

GCTGCGCAT

GCCAT~rGCGA

GATCCAGAT'G

GCCTATCAAG

GGCACAGTCC

CCAGGGAAGG

TAACCAATGT

=IATTGAC

CTGCTTCATT

TTAGTGATCG

ATCGCGGCAGT

CAGCTATAAA

CTTTGCCT

TAGGCACAAC

ATTTAGATGC

TAGCAACAGC

CCGCACCA.AC

CTGCCGATGC

GTACCGCTGA

ATGAP ACGTT

TCCAAACNCC

ATTCAACGGC

GAAATGCAGG

TTACCC'ITCC

CCAGCGGCAC

CCGCACCAAC

CTGCCGATGC

GTACTGCTGA

ATGAAACGTT

TCCAAACGCC

GTAGTACAAC

TTCGCGATGC

CACTrGACTT

ACGCTAGTCG

CAACAG'ITA

ACCCTAACAC

CTGATGCGAA

CGTTAACAGT

TTCCTGCTGA

CTGG77ATCA

ATGGGAACGT

CAGCTGGGAC

ATACAAGTAC

TCGACAAAGT

GCACCACCAT

CGACTGGCCA

ATGTAG ITGC

CTGATGTI'AC

TCACTGGGAC

TTGGTACAAC

CTAATCCTGG

CAACGGAAGT

CAGGAACAAC

AGATTCACAA

TTACAATCAC

AAGATGCTGC

CACCAGTCGC

TAGTAGGAGC

TTGGCATGGC

CCTCAGCTAA

GATTACTCAA

GGAAGTGAAT

TACAGCTCCG

TTAGGGTTA

TCAAGCmGT

TGCAGCCTTG

ATTAGCGGTT

CACGGTTACT

TCGTTTTGTA

AGACGGTGTA

AATCACAGGA

CAATGCCACG

TGGGACAGGA

AACCGCCGTA

AGCGGATGAA

AGGTTACGAA

AGGCACCCTA

CCCAGGTGAA

AGAAAGTACG

AATCACAGGA

CAATGCCACG

TGGGACAGGG

AACCGCCGTA

AGCGGATCCT

AAAAGGCTAT

AGCTGGCACG

AGATTCAGGA

CACGGAAAGT

TAACATCACA

AACAATCGAA

TGGTGATTTT1

GATTGGAAAG

TGCCACGGTT

GGTGACAGGC

GATTGCAACA

GGCAAATGCG

ACCGACAACC

TACTGGGAAT

TGAAGTI'CGT

ATATACTGTG

TAAAAATGAT

CACACCAACA

GGCGGACCCT

GACAACGGAT

TGATACATTA

GACGGTCCCT

TGCCACTGGG

TGAAGCAGGT

TCT1'CCGACG

TGGGA.AAGAA

GACACCTACT

AGCAGAAGTT

AACTACTGGA

CGAAACAATA

154 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. *fecalis Genes.

ACTOTCGTAG

GTCGACTTAG

ATCACTGGAA

ATTGCTGCTA

GTGACACCAG

CCAGCTACAG

CAAGGAAACA

CAATT ITACA

TTCTCCGTTC

ACAGATACAC

GGAGA.ACCAG

GCCGGCTACC

CTATTAAGAA

TTAACAGCGG

GGACATTACA

GGCAATGGTA

GCAACTGGCG

GGT=AGGCA

AGTGGCTACC

GGCTACTTAC

CC TGTCG

CGAAAAATOC

CCACACCAAC

CGGCGAGCC

CAACTGCTAA

GAGAAACGAT

CCGTTATTCC

AAGCCAATGG

ATAGCAGTGA

ATATTGCAGC

AAGGAAATGT

AGATI'AAAAT

TCATCAAAGG

GTCTIGATTGC

GACAAGCGAC

GTACGGCTAC

ACACTGGCGG

GGAATAATGG

CAACAGGTTC

ACCCTAAACT

CTAGCACAGG

CACTCCTTC

AACAGGAAAA

CATTGATTCT

AAAAACCACT

CGAAACCGGC

TACGATTATT

AGCGGATGT

CTATACAGTC

ACAATTATI'C

AGGGTI'AGCA

GAGTCCTAAA

TGCGGCACCA

AkACAGCTGAA

TGTAGGTGCC

TGCTCAACAA

AACCTTCATG

AAATAACGGC

GAATGTCA

TGGCTTAGGT

AAGTACCATC

TGAAAAAGAG

GAGCATGGGA

GAAACTCAGC

ATI'ACCGGAA

ATGATGTCC

CAATATACGG

AGCAAAGATG

GTTTTAGCGG

ACTGGAACT

GCAAGTGGCA

ACAGAAAAAG

ACCACATTTA

ACTCGTTTCTT

CECAAACCCGAA

ACCGATGCTG

AGTTTACTTG

ACGCCAGCCG

AATACAGGCG

AACACAGOTT

TCACTAGGCA

AGTI'ATGGCA

TCTTCAGCCG

ATCATCAAAC

CAGCTACAGC

ATTCTAGTAA

OGACGCAGA

TGACTCTACC

CCAGGTAA

CGCCAACTAT

CTGATCCAAA

ATACAACTAC

AAACGTTAAC

TGACGCCAGC

CAGTITTAGG

TCNTCA.AAT

AAGGCALACTT

CGACAGCTAC

ACCCAACGAA

GCAATACAGG

CAA.ATCCAAA

ATGGCCTCGG

CTGGAAATCA

TGACAACA.AG

GCA-AACGTAA

AACTACACCA

AGGTTACGA.A

CGGAACCATC

AGCTGGCGTA

TGAAAGTCAA

TACGAAGGTT

TGTCACGGT

TrCGACCTACC

CGCACTAACC

CGATATTACG

AACGTCTAAA

TAGTAACCGA

CGCTATCCAA

CGAMGCGCA

TCC I-GAGGA

AAACAATGC

TGGAGGTTICT

TACAAATGGT

CGGGAA.AACA

TTTTGGC

AA.ACTAG

S

S

S

S

5.55 4,

S

S. S 4 5

S.

EF068-2 (SEQ ID NO:258) M KKKIVEDFNR KSQHKKWTKR KMLNLAISSG LLFTSLAIPV S IAVTSGTIS

AVFSGQKQAV

TSGALGNLTG

LAQNWVSNILQ

GSGVNELVDA

NIYFAAGTTS

FTVTVPAGE-A

KGTADANATV

TTFQTPADEA

FTVTI PAGEA

VKGTAEVGTT

PATATTPADV

VTLPTGTTNP

ADPNATIEIR

QTPADEVVAP

LASGKATAKQ

TTIEVRNPDC

DA'IVAAPPTT

TATANEALTA

TTIVEVRDADG

DLATPTIDSI

TPGETITI IS

PYNSSEQLLA

EPEIKIAAPT

TAGQATAQQS

TGGNNGNGSN

YLPSTGEKES

ASAAVLDIEL

LVVPPELRGN

VDIDLTEVNR

DLNAAVQALE

SLLG TI'VTL

EVTAPTITGV

GANETLTAVA

EIRNAGGTVI

TV1TAPTITGV

GANETLTAVA

IEVRDAACT'J

TAPIVDNITG

GDTL'IVIGKD

DADGNVIATG

PSVDKVTGNT

TVNVVAI{NDT

TI IGTI1'TDD

TIVTC'ITATGY

IAKDAAGKES

TVLGMATTGT

TGNSSKCYEI

KDGAGNESQP

SGNITTGrGTF

VSSVLGTSKA

LLATATDGAG

TGSNPNGGSG

SAVTTSLFGA

LSNVTSNNDS

VAAAGSAAIN

QLELVNNIEN

AKGTS IPSNL

PTTVSTPQNL

TGNSTAGYEV

KNASGTEXTP

GTGTADGTG-A

TGNSTAGYEV

KNASGTESTP

LGTAI'CTDG

NSGSGYEITG

NAGNESQPTE

TADGTGSFAV

TQGYQVTGTA

GLESQPTTAM

QCNFTVELPA

QVTGTAEPNV

NPTAFKTPAD

DGKYTVTLEP

TGTAEPKTTI

ATAVIPADVV

SVHIAAGLAT

GYLIKGTAEP

HYSTATI'FMT

LGTTGSGLGS

FVALLASMGI

GTSTSNRWTA

TNVTIDLSKV

LGAASFTAPE

VAAAINAALL

SNNLDARFVC

KGTADANATV

TTFQTPADEA

?IVVPACEA

KGTADANATV

TTFQTPADPN

KYTVTLD)SGT

TADPNTTIEV

VLVPADATVT

NLPACTANAN

ELG~rIEVRA

TPADVTTPTI

GAANPGDTLT

TIEIHNEAGL

PDAPVATPT'J

GKASANETIT

DVRDADGTI I

LAAPTITKVE

EKETLTALTT

NRIIQISNRL

PADPTNPCG

LGNGLGTNGs

IKRKPXN

ANQNQPVNFT

TFLTAVLNAA

TtAADGSYIS

PVKGTIVNVAV

TVVQTDLLDV

EIRNAGGIVI

'IVTAPT ITO V

GANETLTAVA

EIRNAGGAVI

TPVATP IVET

ATANQTLSWV

RDPSGAVIGT

APTVTGVTGN

ETLTALAKDP

TDGTVLGTAT

GDITGDSTTG

VVGKDGDGNE

VIATIGTTDGA

DKI TOSTTNG

VVAYCNATGKE

AATTANETGQ

GNKANGYTVT

DTQGNVSPKT

LRSVIAVGAT

NGNTGNNGN

GYHPKLSTIS

VSGGALADAS

NDLTNVITQI

API SDGLGLV

SGALPLLAVG

NLLATADGVS

GTGTADGTGA

TCNSTAGYEV

KNASGTESTP

GTGTADGTGA

VTGSTTKGYE

AKNASGTESQ

GTSDANGDFT

SVAGYQVTGT

AGNTSTPTTF

TGPTGQYTVT

YEITGTADPN

SQPTEVTVPE

GA-FTITLPTG

YQVVGAAEVG

SQPATATTPV

YTVTLPACVV

GTADPNVIVQ

TFMTPADITG

DAEGNFAIQL

TGGNTGNNGA

YGTGNHGKTIG

155 TABLE 1. Nucleotide and Amino Acid Seqeuences of Efiaecalis Genes.

EF068-3 (SEQ ID NO:259) CTC TGGCACAATC AGTGCATCAG CAGCGGTCTTI GGATATCGAA

CTAITATCAA

GCCGCAA-ACC

TCCGCTGTGT

AATGTACCTG

GTTACTTTI'

ATTACCAGTG

CGTCAATCGG

GAAACGTTAG

GTTTAGCCC

GAG-GCAAAAG

CTTCCTCTCA

GGTGGTI'CAG

TTACCAACTA

GGAACAGTCG

TCCAACATTT

GTAACAGGTA

G'ITGAAATCC

GCGTTTACAG

GCGAAAAACG

GCAACCGTAA

GTTA.AAGGAA

ATAGGCACAG

CCAGCGCCA

CCAACAACGT

GTGACAGGTA

GTTGAGATCC

GCATTTACAG

GCGAAAAACG

ATC ITACGTC

AAAATCAACC

TTAGTGGACA

CACCAGGCAG

TGACTGCCGT

GGGCGTTAGG

AATTIAGTITAA

CAGCTGACGG

AAAATGTTTC

GTACCAGTAT

AAGGCACGGT

GCGTAAATGA

CCGTTTCAAC

?I'CAAACAGA

ATTTTrGCTGC

ATTCAACAC

GAAATGCAGG

TTACCGTTCC

CCAGCGGNAC

CCGCACCA.AC

CTGCCGATC

GTACCGCTGA

ATGAAACGTT

TCCAAACACC

A'TCAACAGC

GAAATGCAGG

'ITACCA'ITCC

CCAGCGGTAC

AAATAATGAC

AGTTAATTTC

AAAACAAGCG

CCCAGCAATC

TTTGAATG-CA

GAATTTAACT

TAACATTGAA

CTCATACATT

AAACATCTTA

CCCAAGTAAT

A.AACGTGGCT

GTTAGTGGAT

ACCTCAAAAT

TCTTITAGAC

AGGCACTACT

AGGT'rACGAA

AGGCACCCTA

CGCAGGTGAA

AGAAAGNACG

AATCACAGGA

CAATGCCACG

TGGGACAGGA

AACCGCCGTA

AGCGGATGAA

AGGTTACGAA

AGGTGCCGTG

CGCAGGTGAA

AGAAAGTACG

AGTCGCACN'

ACGG'ITCTC

GTGTTAGTGG

AATACCAATG

GCCAATGAT'

GGTGTTGATA

AACP'rAGGTG

AGTGCACCGA

CAAGATTTGA

C ITGTCGCCG G TTCAGGTG

GCTTCTTTAC

TTATCCAATA

GTTAATTTAT

AGTGAAGTAA

GTTAAAGGAA

ATAGGCACAG

GCAGGCGCCA

CCAkACAACGT

GTGACACGTA

GTTGAAATCC

GCGTTTACAG

GC-GAAAAACG

GCAACCGTAA

GTTAAAGGAA

ATAGGTACAG

GCACCTGCGA

CCAACAACGT

CAACGACGTAA

GTGGCGC~rT

TTCCTCCTGA

TCACGATTGA

TAACCAATGT

TTGATTGAC

CTGC=CAT

TTAGTrCATGG

ATGCGG'CAGT

CAGCTATAAA

CTT~TGCCT~TT

TAGGCACAAC

ATTTAGATGC

TAGCAACAC

CCGCACCAAC

CTGCCGATGC

GTACCGCTGA

ATGAAACGTT

TCCAAACNCC

ATTCA-ACGGC

GAAATGCAGG

TTACCGTTCC

CCAGCGGCAC

CCCCACCAAC

CT'GCCGATGC

GTACTGCTGA

ATGAAACGTT

TCCAAACGCC

TCGTTGGACA

AGCAGATGCT

GTTAAGAGGA

TC=TCAAAA

GATTACTCAA

GGAAGTGAAT

TACAGCTCCG

=ITAGGGTTA

TCAAGCTTTG

TGCAGCCTTG

ATTAGCGGTT

CACGGTTACT

TCGTTTTGTA

AGACGGTGTA

AATCACAGGA

CAATGCCACG

TGGGACAGGA

AACCGCCGTA

AGCGGATGAA

AGGTTIACGAA

AGGCACCGTA

CGCAGGTGAA

AGAAAGTACG

AATCACAGGA

CAATGCCACG

TGGGACAGGG

AACCGCCGTA

a a a a a a a a EF068-4 (SEQ ID NO:260) TSGTIS ASAAVLDIEL LSN JTSNNDS GTSTSNRWTA ANQNQPVNFT VSGCALADAS a a a,

AVFSGQKQAV

TSGALGNLTG

LAQNVSN~ILQ

GSGVNELVDA

NIYFAAGTTS

F'IVTVPAGEA

LVVPPELRGN

VDIDILTEVNR

DLNAAVQALE

SLLGTTTVTL

EVTAPTITGV

GANETLTAVA

VAAAGSAAIN

QLELNMIEN

AKOTS IPSNL

P'IIVSTPQNL

TGNSTAGYEV

KNASGTEXTP

TNVTIDLSKV

LGAASFTAPE

VA.AAINAALL

SNNLDARFVG

KGTADANATIV

TTFQTP

TFLTAVLNAA

TLAADGSYI S

PVKGTVNVAV

TVVQTDLLDV

EIRNAGGIVI

NDLTNVITQ I AP ISDOLGLV

SGALPLLAVG

NLLATADGVS

GTGTADGTGA

EF069-1 (SEQ ID NO:261)

TAGGCGAAGC

ATGAAAAAGA

CGCAAGATGC

GTAAGTATAG

CTATI'ATCAA

GCCGCAAACC

TCCGCTGTT

AATGTAGCTG

GTTACTT

TAATCATCTT

AAATTG'IIGA

TAATTAGC

CTG'ITACCTC

ATGTTACGTC

AAAATCAACC

TTAGTCGACA

CAGCAGGCAG

TGACTGCCGT

GGTAFIATC

GGATFFI'AAT

AATATCAAGT

TGGCACAATC

AAATAATGAC

AGTTAATTTC

AAAACA6AGCG

CGCAGCAATC

TTTGAATGCA

GTTrATTFrA

CGGAAAAGTC

GGTTTATI'AT

AGTGCATCAG

AGTGGCACT

ACGGTTTCTC

GTCTTAGTGG

AATACCAATG

GCCAATGATT

AAGAAAAGAG

AGCATAAAA-A

TTACGTCATT

CAGCGGTC T

CAACGAGTAA

GTGGCGCTTI

TI'CCTCCTGA

TCACGArM'A

TAACCAATGT

GGACGATCAG

ATGGACAAAA

AGCAATCCCT

GGATATCGAA

TCGTTGGACA

AGCAGATGCT

GTTAAGAGGA

TCTTTICAAAA

GATTACTCAA

156 TABLE 1. Nucleotide and Am~ino Acid Seqeuences orE. fcaecalis Genes.

ATTACCAGTG

CGTCA.ATTGG

GAAACGTTAG

GTTTTAGCCC

GAGGCAAAAG

CTTCCTGTCA

GGTGGTTCAG

TTACCAACTA

GGAACAGTCG

TCCAACATTT

GTAACAGGTA

G I1TGAAATCC

GCGTTTACAG

GCGAAAAACG

GCAACCGTAA

GTTAAAGGAA

ATAGGCACAG

GCAGGTGCCA

CCAACAACGT

GTGACAGGTA

GTITGAGATCC

GCA ITTACAG

GCGAAAAACG

AATACC CC CC

GAGGTCAAAG

GTCCTTGGTA

ACAGCAACAG

CAACCAGCAA

GGCAACTCTG

GTCGTGATC

ACTGTAACC

GATA.ACGCGG

ACAGCACCAA

ACCGCTGATC

GGGACTGCCG

AATGAAACAT

TTCCAAACAC

ACA.ACACAAG

GCAACAGACG

ACGTTAGCTT

ACTGGACTTG

ATTGGTGACA

A.ATACCACCA

GATCAAGGAA

ACAGTTGTTG

GAAGATGCAA

TATCAAGTAA

TAGITATTG

GGCACAGCAA

AGTAATCCG.A

GTTGACAAAP

GGTACAACAC

ACTGATGCP

ACTGTCGTAC

GGGCGTTAGG

AATTACTTAA

CAGCTGACGG

AAAATGTTTC

GTACCAGTAT

AAGGCACGGT

GCGTAAATGA

CCCTTTCAAC

TTCAAACAGA

ATTTGCTGC

ATTCAACAC

GAAATGCAGC

TTACCGTTCC

CCAGCGGNAC

CCGCACCAAC

CTGCCGATGC

GTACCGCTGA

ATCAAACGTT

TCCAAACACC

ATTCAACAGC

GAAATCCAG

TTACCATTCC

CCAGCGGTAC

TCGCGACGCC

GGAC~rCTGA

CTGCAACAAC

CAAATCAAAC

CGGCGACAAC

G'ITCGGGTTA

CATCTGGGGC

TACCAACGCG

GAAATGAAAC

CTGTAACAGC

CCAATGCTAC

ATGGGACTGC

TGACAGCGT'I

CACCAGATC;

GATATCAAG]

GAACAG=IT

CAGGAAAAGC

AGAGTCAAC(

TTACTGGAG)

TTGAAGTACC

ACTTTACTG'.

GAAAAGACG(

*CCGTAGCAG(

*CCGGCACGG(

CTACGGGAA(

CAGCTAACGA

*CTGCTTTCAX

*TCACTGGTA(

TTGAGGTGCI

AATACACAG'

CGAAAAATG

GAATTTA.ACT

TAACATIMAA

CTCATACATT

AAACATCTTA

CCCA.ACTAAT

AAACGTGGCT

GTTAGTGGAT

ACCTCAAAAT

TCTTTTAGAC

AGGCACTACT

AGGTTACGAA

AGCACCGTA

CGCAGGTGAA

AGAAAGNACG

AATCACAGGA

CAATGCCACG

TGGGACAGGA

AACCCCGTA

AGCGGATGAA

AGGTTACGAA

AGGTGCCGTC

CGCAGGTGAA

AGAAAGTACC

AATTCTTrCAG

AGTTGGCACC

TGGAACTGAC

GCTGAGCGTT

ACCAGCTGAT

TGAAATTACA

AGTCATTGCT

AACGACCAAT

TCAACCGACT

AGTAACAGGI

CATCGAAATI

TTCCTTTGC!I

AGCCAAAGA I AGTAGTCGCb

GACAGGTACC

ACGCACCGC)

AACACCTA-A

AACTACAGC']

k. TTCAACAACI

GAACCCAGAJ

r GGACCTTCCI

TGACGGCAA'

ACCAACTGT(

AGAGCCAAAN

GACT'GATGG'

k. AGCCTAAC'.

IN AACACCTGC' 3CACGACAAA( 3 TGACGCCGA' T' GACTTTAGA

CAACAGGAAA

GdTGTTGATA

AACTTAGCTG

AGTGCACCGA

CAAGATTTGA

CTTGTCGCCG

GTTTCAGGTG

GCTTC-TTAC

TTATCCAATA

GTTAATTTAT

AGTGA-AGTAA

9_TTAAAGGAA

ATAGGCACAG

GCAGGCGCCA

CCAACAACGT

GTGACAGGTA

GTTGAAATCC

GCGTTTACAG

GCGAAAAACG

GCAACCGTAA

GTrAAAGGAA

ATACCTACAG

GCAGGTGCGA

CCAACAACGT

ACTGTAACAG

ACCATTGAGG

GGAAAATATA

GTAGCGAAAA

GTCACTGC C:

GGAACAGCAG

ACAGGTACCT

CCTGGGGATA

GAAGTCCTTG

AATTCAG'TC

CGTGATGCAG

GTGAACCC

CCTGCTGCCP

CCGCCAAGCTC

GCTGAAC'TrC

ACAACTGCAC

cAAACAGTC) 7 ATIACACCCC 7GGTTATGAAJ

CCGAACAATTI

k GCGACCC r GAAAGTCAAC

'ACGACTGTTJ

P GTCACCA'IT r' GCTGGCGCA' r GCcATTGCGJ r GATCC.AGAT( :GGcTATCAA( r GGCACAGTC,

:CCAGGGAAGI

A, GAAAGTCAG TTGATrTGAC CTC 1CATT

TTAGTGATGG

ATGCGGCAGT

CAGCTATAA.A

CTITGCC FIT

TAGGCACAAC

ATTTACATCC

TAGCAACAGC

CCGCACCAAC

CTGCCGATGC

GTACCGCTGA

ATGAAACGTT

TCCAAACNCC

A'ITCAACGGC

GAA.ATGCAGG

TTACCGTTCC

CCAGCGGCAC

CCGCACCAAC

CTGCCGATCC

GTACTGCTGA

ATGAAACGTT

TCCAAACGCC

GTAGTACAAC

TTCGCGATGC

CAGTIGACTTT

ACGCTAGTGG

CAACAGTTGA

AC CCTA.ACAC

CTGATGCGAA

CGTTAACAGT

TTCCTGCTGA

CTGG'!FrATCA ATrCCCAACGT

CAGCTGGGAC

ATACAAGTAC

TCGACAAAGI

GCACCACCAU

CGACTGGCCP

ATGTAGTTGC

;CTC;ATCTTAC

k TCACTGGGAC k TTGGTACAAC

CTAATCCTC

:CAACGGAAG'J

CAGGAACAAC

2AGATTCACAJ r TTACAATCA( 4, AAGATGCTGC

CACCAGTCC(

S TAGTAGGAG( CT'rGGCATGG( C CCTCAGCTA C CAGCTACAC(

GGAAGTGAAT

TACAGCTCCG

TTTAGGGTTA

TCAAGC'rrTG TGCAGCC ITG

ATTAGCGGTT

CACGGTTACT

TCGTTTTGTA

AGACGGTGTA

AATCACAGGA

CAATGCCACG

TGGGACAGGA

AACCGCCGTA

ACCGGATGAA

AGGTTACGAA

AGGCACCGTA

CGCAGGTGAA

AGAAAGTACG

AATCACAGGA

CAATGCCACG

TCCGACAGGG

AACCGCCGTA

AGCGGATCCT

AAAAGGCTAT

AGCTGGCACG

AGATTCAGGA

CACGGAAAGT

TAACATCACA

AACAATCGAA

TGGTGATTTT

GATTCGAAAG

TGCCACGGTT

GGTGACACCC

GATTCGCAACA

GGCAA.ATGCG

ACCGACAACC

TACTGCAAT

*TGAAGTTCGT

ATATACTGTG

TAAAAATGAT

CACACCAACA

GGCGGACCCT

GACAACGGAT

TGATACATTA

GACGGTCCCT

TCCCACTGGG

TGAAGCAGGT

TCTTCCGACG

TGGGAAAGA6A

GACACCTACT

AGCAGAAGIT

AACTACTGGA

k. CGAAACAATA

-AACTACACCA

a a.

157 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecali-v Genes.

GTCGACTTAG

ATCACTGGAA

ATTGCTGCTA

GTGACACCAG

CCAGCTACAG

GAAGGAAACA

CAATTTTACA

TTCTCCCTTC

ACAGATACAC

GGAGAACCAG

GCCGGCTACC

CTATTAAGAA

TTA.ACAGCGG

GGACATTACA

GGCAATGGTA

GCAACTGGCG

GGTTTAGGCA

AGTGGCTACC

GGCTACTTAC

GCCTTTGTCG

CCACACCAAC

CGGCGGAGCC

CAACTGCTAA

GAGAAACGAT

CCGTTArrCC

AACCCAATCG

ATAGCAGTGA

ATATTGCAGC

AAGGAAATGT

AGATrAAAAT

TCATCAAAGG

GTGMTTGC

GACPLAGCGAC

GTACGGCTAC

ACACTOCCOG

GGAATAATGG

CAACAGGTTC

ACCCTAAACT

CTAGCACACG

CACTCCTTGC

CATTGATTCT

AAAAACCACT

CGAAAC CCCC

TACGATTATT

AGCGGATGTT

CTATACAGTC

ACAATTATTG

AGGGTTAGCA

GAGTCCTAAA

TGCGGCACCA

AACAGCTGAA

TGTAGGTGCC

TGCTCAACAA

AACCTTCATG

APLATAACGGC

GAATGGTTCA

TGGCTTAGGT

AAGTACCATC

TGAAA.AAGAG

GAGCATGGGA

ATTACCGGAA

ATTGATGTCC

CAATATACC

AGCAAAGATG

GT'rTTAGCGG

ACTGGAACTC

GCAAGTGGCA

ACAGAAA-AAG;

ACCACATTTA

ACTG'ITCTT

CCAAACCGAA

ACCGAtCCTG

AGTTTACTTG

ACGCCAGCCG

AATACAGGCG

AACACAGGTI'

TCACTAGGCA

AGTTATGGCA

TCTTCAG CCC

ATCATCAAAC

ATI'CTA*TAA

GTGACGCAGA

TGACTCTACC

GCGCAGGTAA

CGCC-AACTAT

CTGATCCAA-A

ATACA-ACTAC

AAACGTTAAC

TGACGCCAGC

CAGTTTAGG

TCATTCAAAT

AAGGCAACTT

C:GACAGCTAC

ACCCAACGAA

GCAATACAGG

CAAATCCAAA

ATGGCCTCGG

CTGGAAATCA

TGACA.ACAAG

GCAAACG"AA

AGGTTACGAA

CGGAACCATC

AGCTGGCGTA

TGAAAGTCAA

TACGAAGGTT

TGTCACGGTT

TGGAGGTACC

CGCACTAACC

CGATATTACO

AACGTCTAAA

TAGTAACCGA

CGCTATCCAA

CGATGGCGCA

TCCTGGAGGA

AAACAATGGC

TGGAGGTTCT

TACAA.ATGGT

CGGGAAAACA

TTTGTTTC

AAACTAG

EF069-2 (SEQ ID NO:262) M KKKIVEDFNR KSQHKKWTKR KMLNLAISSG LLFTSLAIPV *0 .q ~0 *0 S IAVTSGTIS

AVFSGQKQAV

TSGALGNLTG

LAQNVSNI LQ

GSGVNELVDA

NIYFAACTTS

F'IVTVPAGEA

KGTADANATV

TTFQTPADEA

FTVTI PAGEA

XTKGTAEVGTT

PATATTPADV

VTLPTGTTNP

ADPNATIEIR

QTPADEVVAP

LASGKATAKQ

TT-IEVRNPIDC

DATVAAPTVT

TATANEALTA

T'IVEVRDADG

DLATPTIDS I

TPGETITIIS

FYNSSEQLLA

EPEIKIAAPT

TAGQATAQQS

TGGNNGNGSN

YLPSTGEKES

ASAAVLDIEL

LVVPPEJRGN

VDI DLTEVNR

DLNAAVQALE

SLLGTTTVTL

EVTAPTITGV

GANETiLTAVA

EIRNAGGIVI

TVTAPTITGV

GANETLTAVA

IEVRDAAGIV

TAPTVDNITOG

GDTLTVIGKD

DADGNVIATO

PSVDKVTGNT

TVNVVAKNDr TI IGT'flTDD

TVTGTTATGY

IAKDAAGKE-S

TIVLGMATTGT

TGNSSKGYEI

KDGAGNESQP

SGNTTTGGTF

VSSVLGTSKA

LLATATDGAG

TGSNPNCGSG

SAVTISLFGA

LSNVTSNNDS

VAAAGSAAIN

QLELVNNIEN

AKGTS IPSNL

PTTVSTPQNL

TGNSTAGYEV

KNASGTEXTP

GTGTADGTGA

TGNSTAGYEV

KNASGTESTP

LGTATTGTDC

NSGSGYEITG

NAGNESQPTE

TADGTGSFAV

TQGYQVTGTA

GLESQPT'AM

QGNFTVDLPA

QVTGTAEPNTV

NPTAFKTPAD

DGKYTVTLEP

TGTAEPKTTI

ATAVI PADVV SVH IAAGLAT

GYLIKGTAEP

HYSTATTFMT

LGTTGSGLGS

FVAT-LASMG I

GTSTSNRWTA

TNVTIDLSKV

LGAASFTAPE,

VAAAINAALL'

SNNLDARFVG

KGT ADANATV

TTQTPADE-A

FTVTVPAGEA

KGTADANATV

TTFQTPADPN

KYTVTLDSGT

TADPNTTIEV

VLVPADATVT

NLPAGTANAN

ELGTTIEVRA

TPADVTTPTI

GAANPCDTLT

TIE IHNEAGL

POAPVATPTV

GKASANETIT

DVRDADGTI I

LAAPTITKVE

EKETLTALTT

NRIIQISNRL

PADPTNPGGG

LGNGLGTNGS

IKRKRKN

ANQNQPVNFT

TFLTAVLNAA

TLAADGSYIS

PV-XGTVNVAV

'IVVQTDLLDV

EIRNAGGIVI

TVTAPTITGV

GANETLTAVA

E IRNAGGAVI TPVATP IVET

ATANQTI.SVV

RDPSGAVIGT

APTVTGVTGN

ETLTALAKIJP

TIDGTVLGTAT

GDITGDSTTG

VVGKIDC DNE

VIATGTTDGA

DKITGSTTNG

VVAKNATGKE

AATTA.NETGQ

GNKA-NGYTVT

DTQGNVSPKT

LR.SVIAVGAT

NGNTGGNNGN

GYHPKLSTIS

VSGGALADAS

NDLTNVITQ I

APISDGLGLV

SCALFLLAVG

NLLATADGVS

GTGTADGTGA

TCNSTAGYEV

KNASGTESTP

GTGTADGTGA

VTGSTTKGYE

AKNASGTESQ

GTSDANGDFT

SVAGYQVTGT

AGNTSTPTTF

TGPTGQYTVT

YEITGTADPN

SQPTEVTVPE

GAFTITLPTG

YQVVGAAEVG

SQPATATTPV

YTVTLPAGVV

GTADPNVTVQ

TFMTPADITG

DAEGNFAIQL

TGGNTGNNGA

YGTGNHGKTG

EF069-3 (SEQ ID NO:263) 158 TABLE 1. Nucleotide and Amino Acid Seqeuenccs of E. faecalis Genes.

AGGTGAA GCAGGTGCGA ATGAAACGTI' AACCGCCGTA

GCGAAAAACG

AATACCCCCG

GAGGTCAAAG

GTCCI'GGTA

ACAGCAACAG

CAACCAGCAA

GGCAACTCTG

GTTCGTGATC

ACTGTAACC

GATAACGCCG

ACAGCACCAA

ACCGCTGATC

GGGACTCCC

AATGAAACAT

TTrCCAAACAC

ACAACACAAG

GCAACAGACG

ACGTTAGCTT

ACTGGACTTG

ATTGGTGACA

AATACCACCA

GATCA.AGGAA

ACAGTTC'Frc

GAAGATGCAA

CCAGCGGTAC

TGGCGACGCC

GGACTCCTGA

CTGCAACAAC

CAAATCAAAC

CGGCGACAAC

GTTCGGGTTA

CATCTGGGGC

TACCAACGGG

GAAATGAAAG

CTGTA-ACAGd

CGAATGCTAC

ATGGGACTC

TGACAGCGTT

CAGCAGATGA

GATATCAAGT

GAACAGT

CAGGAAAAGC

AGAGTCAACC

TTACTGGACA,

TTGAAGTACG

ACTTTACTGT

GAAAAGACG

CCGTAGCAGC

AGAAAGTACG

AA ITTGAG

AGTTGGCACC

TGGAACTGAC

GCTGAGCGTT

ACCACCTGAT

TGAAAITACA

AGTCAT'GGT

AACGACCAAT

TCAACCGACT

AGTAACAGGT

CATCGAAATT

TTCCTTTCCT

AGCCAAAGAT

AGTAGTGGCA

CACAGGTACC

AGGCACCGCA

AACAGCTAAA

AACTACAGCT

TTCAACAACT

GAACCCAGAT

GGACCTTCCA

TGACCGCAAT

ACCAACTGTG

CCA.ACAACGT

ACTGTAACAG

ACCATTGAGG

GGAA.AATATA

GTAGCGAAAA

GTCACTGCAC

GGAACAGCAG

ACAGGTACCT

CCTGGGGATA

CAAGTCCTTG

AATTCAGTT'G

CGATGCAG

GTGAACCTTC

CCTGCTGGCA

CCGCCAAGTG

GCTGAACTTG

ACAACTGGAC

CAAACAGTGA

ATGACACCCG

GTATGAAA

GGAACAATTA

GCGGGAGCCG

GAAACTCA.AC

ACGACTGTTA

TCCAAACOCC

GTAGTACAAC

TTCGCGATGC

CAGTrGACTTT

ACGCTAGTGG

CAACAG'ITGA

ACCCTAACAC

CTGATGCGA.A

CGTTAACAGT

TTCCTGCTGA

CTGGTATCA

ATGGGAACGT

CAGCTGOAC

ATACAAGTAC

TCGACAAAGT

GCACCACCAT

CGACTGGCCA

ATGTAGTTGC

CTGATG'ITAC

TCACT=CAC

TTGGTACAAC

CTAATCCTGG

CAACGGAAGT

CAGGAA

AGCGGATCCT

AAAAGGCTAT

AGCTGGCACG

AGATTCAGGA

CACGGAAAGT

TAACATCACA

AACAATCGAA

TGGTGATTTTI

GATTGGAAAG

TGCCACGGTT

GGTGACAGGC

GATTGCAACA

GGCAAATGCG

ACCGACAACC

TACTGGGAAT

TGAAGTTCGT

ATATACTGTG

TAAAATGAT

CACACCAACA

GGCGGACCCT

GACAACGGAT

TGATACATTA

GACGGTCCCT

S

S.

EF069-4 (SEQ ID NO:264) AGEA GANETLTAVA KNASGTEXTP TTFQTPADE-A TVTAPTITGV TGNSTAGYEV

KGTADANATV

TTFQTPADEA

FTVTIPAGEA

VKGTAEVGTT

PATAT1'PADV

VTLPTGTITNP

ADPNATIEIR

QTPADEVVAP

LASGKATAKQ

TTI EVRNPDG

EIRNAGGTVI

TVTAPTITGV

GANETLTAVA

I EVRDAAGTV

TAPTVDNITG

GDTLTVICKD

DADGNVIATG

PSVDKVTGNT

TVNVVAKNDT

TI IGTTTTID

GTGTADGTGA

TGNSTAGYEV

KNASCTESTP

LGTATI'GTOG

NSGSGYEITG

NAGNESQPTE

TADGTGSFAV

TQGYQVTGTA

GLESQPTTAM

QGNFTVDLPA

FTVTVPAGEA

KGTADANATV

TTFQTPADPN

KYTVTLDSGT

TADPNTT 1EV

VLVPADATVT

NLPAGTA.NAN

ELGTTIEVRA

TPADVTTPTI

GAANPGDTLT

GANETLTAVA KNASGTESTP EIRNAGGAVI GTGTADGTGA TPVATPIVET VTGSTTKGYE ATANQTLSVV AXNASCTESQ RDPSGAVIGT GTSDANGDFT APTVTGVTGN SVAGYQVTGT ETLTALAKDP AGNTSTPTTF TDGTVLGTAT TGPTGQYTVT CDITGOSTTG YEITGTADPN VVGKDGrDGNE SQPTEVTVPE DATVAAPTVT TVTGT EF070-1 (SEQ ID NO:265)

TACGGGAAC

ATGAAAAAGA

CGCAAGATGC

GTAAGTATAG

CTATTATCAA

GCCGCAAACC

TCCGCTGTGT

AATGTAGCTC

GTTACTr

ATTACCAGG

TAATGATCTT

AAATTGTTGA

TAATTTAGC

CTGTTACCTC

ATGTTACCTC

AAAATCAACC

TTAGTGGACA

CAGCAGGCAG

TGACTGCCGT

GGCGCGTTAGG

GGTATTTATC

GGATITAAT

AATATCAAGT

TGGCACAATC

AAATAATGAC

AGTTA.ATTTC

AAAACAAGCG

CGCAGCAATC

TTTGAATGCA

GAA'TI'AACT

GTTrTATrA

CGGAAAAGTC

GGTTTATTAT

AGTGCATCAG

AGTGCCACTT

ACGTTCTG

GTGTTAGTGG

AATACCAATG

GCCAATGATT

GGTGTTGATA

AAGAAAAGAG

AGCATAAAAA

TTACGTCATT

CAGCGGTCTT

CAACGAGTAA

GTGGCGCTTT

TTCCTCCTGA

TCACGATTGA

TAACCAATGT

TTGATMTAC

GGACGATCAG

ATGGACAAAA

AGCAATCCCT

GGATATCGAA

TCGTTGGACA

AGCAGATGCT

GTTAAGAGGA

TCTTTCAAAA

GATTACTCAA

GGAAGTGAAT

159 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

CGTCAATTCG

GAAACGTTAG

GTTTTAGCCC

GAGCCAAAAG

CT-TCCTGTCA

GGTGGTTCAG

TTACCAACTA

GGAACAGTCG

TCCAACATTT

GTAACAGGTA

GTTGAAATCC

GCGTTTACAG

GCGAAAAACG

GCAACCGTAA

GTTAAAGGAA

ATAGGCACAG

GCAGGTGCCA

CCAACAACGT

GTGACAGGTA

GTTGAGATCC

GCATTACAG

GCGAAAAACG

AATACGCCCG

GAGCTCAAAG

GTCCTTGGTA

ACAGCAACAG

CAACCAGCAA

GGCAACTCTG

GTTCGTGATC

ACTGTAACGC

GATAACGCGG

ACAGCACCAA

ACCGCTGATC

GGGACTGCCG

AATGAAACAT

TT'CCAAACAC

ACAACACAAG

GCAACAGACG

ACGTTAGCTT

ACTGGACTTG

ATTGGTGACA

AATACCACCA

GATCAAGGAA

ACAGTTGTTG

GAAGATGCAA

TATCAAGTAA

TTAGTTATTG

GGCACAGCAA

AGTAATCCGA

GTTGACAAA

GGTACAACAG

ACTGATGGCA

ACTGTCGTAG

GTCGACTTAG

AATTAGTTAA

CAGCTGACCG

AAAATGTTTC

GTACCAGTAT

AAGGCACGGT

GCGTAAATGA

CCG=TCAAC

TTCAAACAGA

ATGCTC

ATCAACAGC

GAAATGCAG

TTACCGTTCC

CCAGCGGNAC

CCGCACCAAC

CTGCCGATGC

GTACCGCTGA

ATCAA.ACGTT

TCCAAACACC

A ETCAACAGC

GAAATG;CAG

TTACCATTCC

CCAGCGGTAC

TCGCGACGCC

GGACTGCTr-A

CTGCAACAAC

CAAATCAAAC

CGGCGACAAC

GTTCGGGTrA

CATCTGGGGC

TACCAACCCG

GAAATGAAAG

CTGTAACACG

CGA.ATCGCTAC

A~rGGACTGG

TGACAGCGTT

CAGCAGATGA

GATATCAACT

GAACAGTT

CACCAAAAC

AGAGTCAACC

TTACTGGAGA

TMAAGTACG

ACTTTACTGT

GAA.AAGACGG

CCGTAGCAGC

CCGGCACGGC

CTACGGGAAC

CAGCTAACGA

CTCTTCAA

TCACTGGTAG

TTGAGGTCCG

AATACACACT

CGAAAAAT~c

CCACACCA.AC

TAACA'ITGA

CTCATACATT

AAACATCTTA

CCCAAGTAAT

AAACGTCGCT

GTTAGTGGAT

ACCTCAAAAT

TC LTTTACAC

AGGCACTACT

AGGTTACGAA

AGCCACCGTA

CGCAGGTGAA

AGAAAGNACG

AATCACAGGA

CAATGCCACG

TGGGACAGGA

AACCGCCCTA

AGCGGATGAA

AGGTTACGAA

ACGTGCCGTC

CGCAGGTGAA

AGAAAGTACG

AATTGTTCAG

AGI'CGCACC

TGGAACTGAC

GCTGAGCGT

ACCACCTGAT

TGAAATI'ACA

AGTCAITGGT

AACGACCAAT

TCAACCGACT

AGTAACAGGT

CATCGAAAT

TTCC TTGCT

AGCCAAAGAT

ACTAGTGGCA

GACAGGTACC

AGGCACCGCA

AACAGCTAAA

AAC'TACAGCT

TTCAACAACT

GAACCCAGAT

GGACCTTCCA

TGACGGCAAT

ACCAACTGTG

AGAGCCAAAT

GACTGATGGT

AGCCTTAACT

AACACCTGCT

CACGACAAAC

TGACGCCGAT

CACTTTAGAG

AACAGGAAAA

CATTGATTCT

AACTT2AGGTG CTGCTTCATT TACAGCTCCG AGTGCACCGA TTAGTGATGG =TAGGGTTA CAAGATTTGA ATCCGCAGT TCAAGCTITG CTTGTCGCCG CAGCTATAAA TGCAGCCTTG GT'TTCAGGTG CTTTGCCTTT ATTAGCGGTT GCTTCTI'TAC TAGGCACAAC CACGG'ITACT TTATCCAATA ATTTAC-ATGC TCGTTTTGTA GTTAATTTAT TAGCAACAGC AGACGGTGTA AGTGAAGTAA CCGCACCAAC AATCACAGGA GTTAAAGGAA CTGCCGATGC CAATGCCACG ATAGGCACAG GTACCGCTGA TGGGACAGCA G&AGGCGCCA AT;AAACGTT AACCGCCCGTA CCAACAACGT TCCAAACNCC AGCGGATGAA GTGACAGGTA ATTCAACGGC AGGTTAC'GAA GTTCAALATCC GAAATCCAGG AGGCACCGTA GCGTTTACAG TTACCGTTCC CGCAGGTGAA GCGAAAAACG CCACCCAC AGAAAGTACG GCAACCGTAA CCGCACCAAC AATCACAGGA GTTAAAGGAA CMCCGATGC CAATOCCACG ATAGTACAG GTACTGCTGA TGGGACAGGG GCACGTGCGA ATGAAACGTT AACCGCCGTA CCAACAACGT TCCAAACGCC AGCGGATCCT ACTGTAACAG CTAGTACAAC AAAAGGC TAT ACCATTGAGG TTCGCGATGC AGCTGGCACC GGAAAATATA CAGTGACTTT AGATTCAGGA GTAGCGAAAA ACGCTAGTGG CACGGAAAGT GTCACTGCAC CAACAG'ITGA TAACATCACA GGAACAGCAG'ACCCTAACAC AACAATCGAA ACAGGTACCT CTGATGCGAA TGGTGATTT CCTGGGGATA CGTTAACAGT GATTGGAAAG GAAGTCCTTG TTCCTGCTGA 1GCCACGG T AATTCAGTTG CTGGTTATCA GGTGACAGGC CGTGATCCAG ATGGGAACGT GATTGCAACA GTGAACC=~ CACTCCGAC CGCAAATGCG CCTGCTGGCA ATACAAGTAC ACCGACAACC CCCCAACTC TCGACAAAGT TACTGGGAAT CCTr.AAC7-Ir GCACCACCAT TGAACTTCGT ACAACTGGAC CGACTGGCCA ATATACTGTG CAAACAGTGA ATGTAGTTGC TAAAALATGAT ATGACAC CCG CTGATTAC CACACCAACA GGTTATGAAA TCACTGGGAC GGCGGACCCT GGAACAAITA TTGGTACAAC GACAACGGAT GCGCGAGCCC CTAATCCTG.G TGATACATA GAAAGTCAAC CAACGGAAGT GACGGTCCCT ACGACTGTI'A CAGGAACAAC TGCCACTGGG GTCACCATT Ac3ATTCACAA TGAAGCAGGT GCTGGCGCAT TrACAATCAC TCTTCCGACG GCCATflGCGA AAGA-TGCTGC TGGGAAAGAA GATCCAGATG CACCAGTCGC GACACCTACT GGCTATCAAG TAGTAGGAGC AGCACAAGTT GGCACAGTCC TTGGCATGGC AACTACTGGA CCAGGAAGG CCTCAGCTAA CGAAACAATA GAAAGTCAGC CACCTACAGC AACTACACCA ATTACCCGAA ATTCTALGTAA AGGTTACGAA 160 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

*ATCACTIGGAA

ATTGCTGCTA

GTGACACCAG

CCAGCTACAG

GAAGGAAACA

CAATTTrrACA

TTCTCCGTTC

ACAGATACAC

GGAGAACCAG

GCCGGCTACC

CTATTAAGAA

TTAACAGCGG

GGACATTACA

GGCAATGGTA

GCAACT'GGCG

GTTAG4GCA

AGTGGCTACC

GGCTACTTAC

GCCTTTGTCG

CGGCGGAGCC

CAACTGCTAA

GAGAAACGAT

CCGTTATTCC

AAGCCAATGG

ATAGCAGTGA

ATATTGCAGC

AAGGAAATGT

AGATTAAAAT

TCATCAAAGG

GTGTGA'ITGC

GACAAGCGAC

GTACGGCTAC

ACACTGGCGG

GGAATAATGG

CAACAGGTIC

ACCCTAAACT

CTAGCACAGG

CACTCCTTGC

AAAAACCACT

CGAAACCGGC

TACGATI'ATT

AGCGGATGTT

CTATACAGTC

ACAA'ITATTG

AGGCTIAGCA

GAGTCCTAAA

TGCGGCACCA

AACAGCTGAA

TGTAGGTGCC

TGCTCA.ACAA

AACCTT'CATG

AAATAACGGC

GAATGGTTCA

TGGCTTAGGT

AAGTACCATC

TGAAAAAGAG

GAGCATGGGA

ATTGATGTCC

CAATATACGG

AGCAAAGATG

GTTTTAGCGG

ACTGGAACTG

GCAAGTGGCA

ACAGAAAAAG

ACCACATTTA

ACTGTTTCT'

CCAAACCGAA

ACCGATGCTC

AGT=TACTTG

ACGCCAGCCG

AATACAGGCG

AACACAGGTT

TCACTAGGCA

ACTtATGGCA

TCTTCAGCCG

ATCATCAAAC

GTGACGCAGA

TGACTCTACC

GCGCAGGTAA

CCCCAACTAT

CTCATCCAAA

ATACALACTAC

AAACGTTAAC

TGACGCCAGC

CAGI=TAGG

TCATI'CAAAT

ALAGGCAACTI'

CGACAGCTAC

ACCCAACGAA

GCAATACACG

CAAATCCAAA

ATGGCCTCGG

CTGGAAATCA

TGACAACAAC

GCAAACGTAA

CCAACCATC

AGCTGGCGTA

TGAAAGTCAA

TACGAAGGTT~

TGTCACGITr

TGGAGGTACC

CGCACTAACC

CGATATTACG

AACGTCTAAA

TAGTAACCGA

CGCTATCCAA

CGATGGCGCA

TCCTGGAGGA

AAACAATGGC

TGGAGGTTCT

TACAAATGGT

CGGGAAAACA

TTTGT PTGGC

AAACTAG

EFO?O-2 (SEQ ID NO:266) M KKKIVEDFNR KSQHKKWTKR KMLNLAISSG'LLFTSLAIPV

SIAVTSGTIS-

AVPSGQKQAV

TSGALGNLTG

LAQNVSNILQ

GSGVNELVDA

N IYFAAGTITS

FTVITVPAGEA

KGTADANATV

TTFQTPADEA

FTVTI PAGEA VKGTALVGTr

PATATTPADV

VTLPTGTTNP

ADPNATIEIR

QTPADEVVAP

LASGKATAKQ

TTIEVRNPDG

DATVAAPTVT

TATANEALTA'

TTVEVRDADG

DLATPTIDSI

TPGETITI IS

FYNSSEQLLA

EPEIKIAAPT

TAGQATAQQS

TGGNNGNGSN

YLPSTGEKES

ASAAVLDIEL

LVVPPELRGN

VDIDLTEVNR

DLNAAVQALE

SLLGTTTVTL

EVTAPTITGV

GANETLTAVA

EIRNAGGTVI

TVTAPTITGV

GANETLTAVA

IEVRDA.AGTV

TAPTVDNITG

GDTLTVIGKD

DADGNVIATG

PSVDKVTIGNT

TVNVVAKNDT

TI IGTT'ITDD TVTGTTATGy

IAKDAAGKES

IVLGMATI'GT

TGNSSKGYE I

KDGAGNE-SQP

sGN'TTCCGTF

VSSVLGTSKA

LLATATDGAG

TGSNPNGGSG

SAVTTSLFGA

LSNVTSNNDS

VAAAGSAAIN

QLELVNNIEN

AKGTSIPSNL

PTTVSTPQNL

TGNSTACYEV

KNASGTEXTP

GTGTADGTGA

TGNSTAGYEV

KNASGTESTP

LGTATTGTDG

N~SGSGYE ITG

NAGNESQPTE

TADGTGSFAV

TQGYQVTGTA

CLESQPTTAM

QGNFTVDLPA

QVTGTAEPNV

INPTAFKTPAD

DGRYTVTLEP

TGTAEPKTTI

ATAVI PADVV

SVHIAAGLAT

GYLIKGTAEP

HYSTAITFMr

LGTTGSGLGS

FVALLASMGI

GTSTSNRWTA

TNVTIDLSKV

LGAASFTAPE

VAAAINAALL

SNNIJDARFVG

KGTADANATV

TTFQTPADEA

FTVTVPAGEA

KGTADANATV

TTFQTPADPN

KYTVTLDSGT

TAD)PNTTIEV

VLVPADATVT

NLPAGTANAIJ

ELGTTIEVRA

TPADVTTPTI

GAANPGDTLT

TIEIHNE-AGL

PDAPVATPTV

GKASANETIT

DVRDADGTI I

LAAPTITKVE

EKETLTALTT

NRIIQISNRL

PADPTNPGGG

LGNGLGTNGS

IKRKRKN

ANQNQPVNF'

TFLTAVLNAA

TLAADGSYIS

PVKG'IVNVAV

'TVVQTDLLDV

El RNAGGIVI

TVTAPTITGV

GANETLTAVA

EIRNAGGAVI

TPVATP IVET

ATANQTLSVV

RDPSGAVIGT

APTVTCVTGN

ETLTALAXDP

TDGTVLGTAT

GDITGDSTTG

VVGKD)GDCUE

VIATG=rGA DKITGSTrNG

VVAKNATGKE

AATTANETGQ

GNKANGY'IVT

DTQGNVSPKT

LRSVIAVCAT

NGNTGGNNGN

GYHPKLSTIS

VSGGALADAS

NDLTNVITQI

APISDGLGLV

SCALP LLAVG

NLLATADGVS

GTGTADGTGA

TGNSTAGYEV

KNASGTESTP

GTGTADGTGA

VTGSTTKGYE

AKNASGTESQ

GTSDANGDFT

SVAGYQVTGT

AGNTSTPITF

TGPTGQYTVT

YEITGTADPN

SQPTEVTVPE

GAFTITLPTG

YQVVGAAEVG

SQPATATTPV

YTVTTLPAGVV

GTADPNVTVQ

TFMTPADITC

DAEGNFAIQL

TGGNTGNNGA

YGTGNI{GKTG

EF070-3 (SEQ ID NO:267) CGG TCACGGCAAT GAAAGTCAAC CAACGGAAGT GACGGTCCCT 161 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

GAAGATGCAA

TATCAAGTAA

TTAC ETATTIG

GGCACAGCAA

AGTAATCCGA

GTTGACAA.AA

GGTACAACAG

ACTGATGGCA

ACTGTCGTAG

GTCGACTTAG

ATCACTGGAA

ATTGCTGCTA

GTGACACCAG

CCAGCTACAG

GAAGGAAACA

CAATTTTACA

TTCTCCGTTC

ACAGATACAC

GGAGAACCAG

GCCGGCTACC

CTATTAAGAA

TTAACAGCGG

GGACATTACA

GGCAATGGTA

CCAACTCGCG

GGTTTAGGCA

AGTGGCTACC

GGCTACT

CCGTAGCAGC

CCGGCACGGC

CTACGGGAAC

CAGCTAACGA

CTGC'I=CAA

TCACTGGTAG

TTGAGGTGCG

AATACACAGT

CGAAAAATGC

CCACACCAAC

CGGCGGAGCC

CAACTGCTAA

GAGAAACGAT

CCGTTATTC

AAGCCAATGG

ATACCAGTCA

ATATTGCAC

AAGGAAATGT

AGA ITAAAAT

TCATCAAAGG

GTGTGATC

GACAAGCGAC

GTACGGCTAC

ACACTGGCGG

GGAATAATGG

CAACAGCT'C

ACCCTAAACT

ACCAACTGTG

AGAGCCAAAT

GACTGATGGT

AGCCTTAACT

AACACCTGCT

CACGACAAAC

TGACGCCCAT

GACTTAGAG

AACAGGAAAA

CATTGATTCT

AAAAACCACT

CGAAACCGGC

TACGATTATT

AGCGGATGTT

CTATACAGTC

ACAATTATTG

AGGGTTAGCA

GAGTCCTAAA

TGCGCCACCA

A.ACAGCTGAA

TGTAGGTGCC

TGCTCAkACAA

AACCTTCATG

AAATAACGGC

GAATGGTTCA

TGGCTTAGGT

AAGTACCATC

ACGACTGTTA

GTCACCATTG.

GCTGGCGCAT

GCCATTGCGA.

GATCCAGATG

GGCTATCAAG

GGCACAGTCC

CCAGGGAAGG

GAAAGTCAGC

ATTACCGGAA

ATTGATGTCC

CAAkTATACGG

ACCAAACATG

GTTTTAGCGG

ACTGGAACTG

GCAAGTGGCA

ACAGAAAAAG

ACCACATTTA

ACTGTTTICTT

CCAAACCGAA

ACCGATGCTG

AGT~AC=G

ACGCCAGCCG

AATACAGGCG

AACACAGGTr

TCACTAGGCA

AGTTrATGGCA

CAGGA.ACAAC

AGATTCACAA

TTACAATCAC

AAGATG-CTrGC

CACCAGTCGC

TAGTAGGAGC

TTGGCATGCGC

CCTCAGCTAA

CAGCTACAGC

ATTCTACTAA

GTGACGCAGA

TGACTCTACC

GCGCAGGTAA

CGCCAACTAT

CTGATCCAA

ATACAACTAC

AAACGTTAAC

TGACGCCAGC

CAGTTTTAGG

TCATTCAAAT

AAGGCAACTT

CGACAGCTAC

ACCCAACGAA

CCAATACAGG

CAAATCCAAA

ATGGCCTCGG

CTGGAAATCA

TGCCACTGGG

TGAAGCAGGT

TCTTCCGACG

TGGAAAGAA

GACACCTACT

AGCAGAAGT'

AACTACTGGA

CCAAACAATA

AACTACACCA

AGGTTACGAA

CGGAACCATC

AGCTGGCGTA

TGAAAGTCAA

TACGAAGGTT

TGT--ACGGTT

TGGAGGTACC

CGCACTAACC

CGATATTACG

AACGTCTAAA

TAGTAACCGA

CGCTATCCAA

CGATGGCGCA

TCCTGGAGGA

AAACAATGGC

TGGAGGTTCT

TACAAATGGT

CGGCAAAACA

EF70-4 (SEQ ID NO:268) DGOGNE SQPTEVTVPE DATVAAP'IVT 'IVTGTTATGY TATANEALTA IAKDAAGKES TTVEVRDADG TVLGMATTGT DLATPTIDSI TGNSSKGYEI TPGETITIIS KDGAGNESQP FYNSSEQLLA SGNT'rGGTF EPEIKIAAPT VSSVLGTSKA TAGQATAQQS LLATATDGAG TGGNNGNGSN TGSNPNGGSG

QVTGTAEPNV

NPTAFKTPAD

DGKYTVTLEP

TGTAEPKTTI

ATAVI PADVV SVH IAACLAT

GYLIKGTAEP

HYSTATTFMT

LGTI'GSGLCS

TIEIHNEAGL

PDAPVATPIV

GKASANETIT

DVRDADGTI I

LAAPTITKVE

EXETLTALTT

NRIIQISNRL

PADPTNPGGG

LGNGLOTNGS

VIATGTTDGA

DKITGSITNG

VVAXNATGKE

AATTANETGQ

GNKANGYIVT

DTQGNVSPKT

LRSVIAVGAT

NGNTGGNNIGN

GYHPKLSTIS

GAFTITLPTG

YQVVGAAEVG

SQ PATATTPV YTrVTLPAGVV

GTADPNVTVQ

TFMTPADI TO DAErGNFAIQL

TGGNTGNNGA

YGTGNNGKTG

EF071-1 (SEQ ID NO:269)

TAAGTAGAAG

GTCCCGCCAT

ATGGCTTTTT

GTGATTGGTT

CCAATCAACT

TCTGGAAACG

CGAAATTGGT

TGGTCGGGAC

TTATCTGCAG

TAAGAAkAGGA

TAAGTI'TAAC

TTACTTATTT

AGCGGAACCT

CAAATGCTTA

AAACGTAGAA

G'IITAAGCCG

GCATGCTATG

GA'FrCCGATG

TCCCGGCTCT

AGGACCACAC

TGTCTCATAT

CTTTCGCTGA

TGGMAGGGAA

TTTAAAAAAT

ACGGCTTNCG

GCAAGCAATG

AG=TAGACA

TTT'GTCGGAT

TI'GCCGAAGA

CTTATITGA

TA.ATGATITCA

CTTACACCAT

AATTAAITT

ATGAAGTGGC

CTGGI'GGACG

AATI'ACTTCT

cTTrCCTrTC

ACTTGCTTTA

CGAAGCGGAT

TTTACATGAA

CTATATGAAA

AGTGAAACAA

162 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.fiaecalis Genes.

TTAGCTCCTG

GCGCAAATCG

TATGTTAA'Fr

GGAACAGT

ACAGATCCTT

ACAACGCGCG

CTGGCCAAAT

AATTGGCTCG

TGGCCCGTGA

ATGGCATAGT

ATGGTTAT-T

TTTlCNGNAAC

TCAATATGGC

AACGAATAAT

TTTGGCTCAG

CACTCATGAT

AGCGCGTGGG

AGGTGAGACT

GCAGGTTCTT

GCGGCGACAT

AACATTGGTG

TGGATTACAA

GGATTAGTAA

GGTCATTATT

TAGCTAATCA

TTAAAAAAGA

CTGATTTTTC

AAAATCIGTG

AGCGCATMG

CAGCCAGGTA

AAAAGCCTAT

T'ATGCTGCC

TCTGGACGAT

GGGAGATCAT

CACNAGATTI'

EF071-2 (SEQ ID NO:270) MF KKLMIQLALV IGLSLTI PMT AXAYTIEADP INFTYFPGSA SNEL IVLHES GNERNLGPHS LDNEVAY~'l( NWSNAYVSYF VGSGGRVKQL APAGQ IQYGA GSLANQKAYA Q IELARTNNA ATFK KDYAAY VNLJARDLAQN IGADFSLDDG TGYGIVTHDW ITKNWWGDHT DPYGYIJARGG LVKRIGTRFT TGVSXTGETG HYSAR EF071-3 (SEQ ID NO:271) G TTTAAAAAAT TAATGATTCA ACTTGCCT A GTGATTGGT'r

CCAATCAACT

TCTGGAAACG

CGAAATTGGT

TTAGCTCCTG

GCGCAAATCG

TATGTTAATT

GGAACAGGTT

ACAGATCCTT

ACAACGGrCCG

TAAGTTTAAC

TTACTTATTT

AGCGGAACCT

CAAATGCTTA

CTGGCCAAAT

AATTGGCTCG

TGGCCCGTGA

ATGGCATAGT

ATGGTTATTI'

TTITCNGNAAC

GATTCCGATG

TCCCGGCTCT

AGGACCACAC

TGTCTCATAT

TCAATATGGC

AACGAATAAT

TTTGGCTCAG

CACTCATGAT

AGCGCGTGGG

AGGTGAGACT

ACGGCITNCG

GCAAGCAATG

AGTTTAGACA

TTTGTCGGAT

GCAGGTTT

GCGGCGACAT

AACATTGGTG

TGGATT1ACAA

GGATTAGTAA

GGTCA ITAT

CTTACACCAT

AATTAATTGT

ATGAAGTGGC

CTGGTGGACG

TAGCTAATCA

TTAAAAAAGA

CTCGATTTTC

AAAATTGGTG

AGCGCATTGG

CAG-CCAGGT

CGAAGCGGAT

T''IACATGAA

CTATATGAAA

AGTSAAACAA

AAAAGCCTAT

TTATGCTGCC

TCTGGACGAT

GGGAGATCAT

CACNAGATFI

EF071-4 (SEQ ID NO:272) F KKLMIQLALV IGLSLTIPMT AXAYTIE-ADP INFTYFPGSA SNELIVLHES GNERNLGPHS LDNEVAYMKR NWSNAYVSYF VGSGGRVKQL APACQIQYGA GSLANQKAYA QIELARTNNA ATFKXDYAAY VNLARDLAQN IGADFSLDDG TGYGIVTHDW ITKNWGDHT DPYGYLARGG LVKRIGTRFT TGVSXTGETG HYSAR EF072-1 (SEQ ID NO:273)

TAATCAATGA

T=~CACAGC

TITATTGTTCC

CTGCTTCAAA

CCGTTTTATC

GAAACCGGTG

GAAGATGGAG

TATTTATTTG

GTGAT'I~TGC

AAAAATGAAG

CAAGGCTA

ATTTTIAGGAT

CCAGAATCAA

ACGCAAAAC

AAAACGCACG

AAGCATTAGC

CTGATGGTCA

ATTATCGGGG

AGCTTCGTTC

CAACAAATAG

TGGTTTCTT

TTGAAGCGGA

CTGTTCAAGA

AAAATGCCTA

ATCAAGGAGA

ATCAGGAATI'

TTGAGGTAAA

ATGGAFI'TAC

FI'GGTTAAGT

AGAGGCATCG

ATTACCAGAA

CTTAAATGAC

TGAAGGAAAA

AAAACCGATC

TrCA'ITAGCT

AGCACCAGAA

TCCACAAGGG

TGACTTACCA

GCACATTAAC

CCGTTGTCA

AGTGGCTGGA

GCTI'GATTT

ATT-TGCGTCA

CAAGCAAGCG

CAGCAGCAAA

GTCACTTATC

ACGGTCCAAG

GCAGAAGATA

AGCAAAGATT

GTGGTAAAGG

CAATCGTTAA

CCACTTGAAA

TATCAGTTAA

GATAAGGCGG

AAAACAGTTA

TCAATTAAAG

TGCTACTCGC

TTCAAGTTAC

ACACAGGGGA

AAGTCTATGA

AGGCACAGCG

AAACACAGAC

CGCAGCAACG

AAAA.AGCTAG

CGCATATTCA

AAACGGTACT

CGACTCAGAT

ATACAACGTT

CTACAGGTA

AC ETACAAAA

TCTTTTCGGG

GTTGCACAAA

AGAGGGAACG'

TGTGACGGAT

TCAATTAGCA

AATAAATGGA

AGATAPLAGCC

CAACCTAGTA

LTTATATCCA

CGATAAGCAA

TCCAGCGAAT

GACAC ITTTA

CACACTGACG

CTTTGCAAAT

163 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

CAAACAATGA

ATTAACAACG

CGTACAGGCG

GAGGCTGTTT

TATCGTTGGC

I=TCAGTTA

AGG PTATCT

GAACGGACAA

CTGTGTCGTA

A.AGGACAATT

GCAAGTCTTT

TIATCGTCA-A

AAA.AACAAAA

AAGGNNTTAA

CTGAATCAAA

CGAACAGCAC

TCAAATGCGT

AGTCACGGAC

TGTCAAAGTT

AAATCAAGCG

AGCATTAGCT

AAGATGGCCA

CAGAAATTCC

CGTTACATGT

TTAGAAAAGA

AAACATACCT

GATAGTGAAA

GGGGAATACC

AAAAAATTCA

GTACTTCTTG

r 117 1ACGGTG

AATCAATAA

CCGC-TCAACC

TGACTAAAAG

ATGCGAAAAT

TCAATGAAAC

CGTCTAATCA

GAAGA.ATCT

GGAAAAAATT

TGACACTGCG

AGCCACAC-TT

CACCTTGCCA

AGCAAAC'SGG

AGCCOCTCAA

CTGCACCAAA

CTTATGCAAC

EF072-2 (SEQ ID NO:274) MKNARWLSI CVMLLALFGF SQQALAEASQ ASVQVTILHKL LFPCGQLPEQ -QQNTGEEGTL

LQNYRGLNDV

DGVVS FSLAS

NEENAYDLPP

Es -EVKVAG(

NNEGQLVTDK

RWQKEKALA(

TYQVYDVTDP

KDSQQRDKAY

LEKTVLDKQQ

TVTTGYTLTT

HTLTKRA'IVR

KFTSNQAGEF

FYQLRSEGKT

LFVE-AEAPEV

GFNQGEH INY

QKHGFTLDFS

TGGKSF'VKVD

SVKGXKRWPV

VQEAQRQLAE TGATNRk?IA VKEKASNLVV ILPVQDPQGQ QLTTQIPANI LGYQEFRLSD IKDLQNFANQ TMTVSYQMRE.

SENAKITLPE AVFIVKNQAG LLGRNLCTKR LSSESNRNSF EDKTQT INGE

SLTHIHLYPK

KADTTLTLLP

ERTAEPDTAI

EYLNETANGY

YGGKKFLCNE

RTTNSTVTCN Q EF072-3 (SEQ ID NO:275)

ATTACCAGAA

CTGCTTCAAA

CCGTTTI'ATC

GAAACCGGTG

GAAGATGGAG

TATTTATrrG

OTGATTTTGC

AAAAATCAAG

CAAGGCTTTA

ATTAGGAT

CCAGAATCAA

ACGCAAAAGC

CAAACAATGA

ATTAACAACG

CGTACAGGCG

GAGGCTGTTT

TATCGTTGGC

TTTTCAGTTA

AGGTTATCTT

GAACGGACAA

CAGCAGCAAA

ATI'ATCGGGG

AGCTITCGTTC

CAACAAATAG

TGGTTTCTFI

TTGAAGCGGA

CTGTTCAAGA

AAAATv.CCTA

ATCAAGGAGA

ATCAGGAATI'

TTGAGGTAAA

ATGGATTAC

CTGTGTCGTA

AAGGACAAT'

GCAAGTCT

TI'ATCGTCAA

AAAAAGAAAA

AAGGNNTTAA

CTGAATCAAA

CGAACAGCAC

ACACAGGGGA

CTTAAATGAC

TGAAGGAAAA

AAA.ACCGATC

TTCATTAGCT

AGCACCAGAA

TCCACAAGGG

TGACTTACCA

GCACATTAAC

CCGTTTGTCA

AGTGGCTIGGA

GCTTGATTT

TCAAATGCGT

AGTCACGGAC

TGTCAAAGT'

AAATCAAGCG

AGCATTAGCT

AAGATGGCCA

CAGAAAT'CC

CGTACATGT

AGAGGAACG

GTCACTTATC AAGTCTATGA ACGGTCCAAG AGGCACAGCG GCAGAAGATA AAACACAGAC AGCAAAGATT CGCAGCAACG GTGGTAAAGG AAAA.AGCTAG CAATCGTTAA CGCATATTCA CCACTTGAAA AA.ACGGTACT TATCAGTAA CGACTCAGAT GATAAGGCGG ATACAACGTT AAAACAGTTA CTACAGCCTA TCAATTA.AAG ACTTACAAAA TTAGAAAAGA CCGCTGAACC AAACATACCT TGACTAAAAG GATAGTGAAA ATGCGAAAAT GGGGAATACC TCAATGAAAC AAAAAATTCA CGTCTAATCA GTACITCTTG GAAGAAATCT TITTACGGTG GGAAAAAATT

A

TGTGACGGAT

TCAATTAGCA

AATAA.ATGGA

AGATAAACCC

CAACCTAGTA

T'rATATCCA

CGATAAGCAA

TCCAGCGAAT

GACACTTTTA

CACACTGACG

C71TGCAAAT

TGACACTGCG

AGCCACAGTI

CACCTTGCCA

AGCAAACGGG

AGCCGGTGAA

CTGCACCAAA

C'IATGCAAC

EF072-4 (SEQ ID NO:276) QLPEQ QQNTGEEGTL LQNYRGLNDV TYQVYDVTDP DCVVSFSLAS KDSQQRDYAY NEENAYDLPP LEKTVLDKQQ ESIEVKVAGX TV'ITGYTLTT NNEGQLVTDK HTLTKRANVR RWQKEKALAK KFTSNQACEF

RTTNSTVTC

FYQLRSEGKT

LFVEAkEAPEV GFNQGEH1INY

QKHGFTLDFS

TGGKSFVKVD

SVKGXKRWPV

VQEAQRQLAE

VKEKASNLVV

QLTTQIPANI

IKDLQNFANQ

SENAKITLPE

LLGRNLCTKR

TGATNRKP IA

ILPVQDPQGQ

LGYQEFRLSD

TMIVSYQMRL

AVFItVINQAG

LSSZSNRNSF

EDKTQT IN~GE

SLTHIHLYPK

KADTTLTLLP

EKTAEPDTAI

EYLNETANGY

YGGKKFLCNE

164 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

EF073-1 (SEQ ID NO:277)

TAAATGAACA

ATTGCTGTCG

TTCGTTCTCT

ACGAAAGATA

TTCAGAAGTA

CGTTCAACTA

CTGTTTAAAG

ACACATTTAC

CTAAAAAACA

TCAAAATI'AG

GTCGAAATGT

CAAGTAAACG

AATTAAATAC

CTAGAGAATA

ATCCAAAAAA

AAGAAGCCCA

CAATGAGCAC

AATTACGGC

AGTTAGTGAA

CAAATATCGT

AACA.AACGTA

TTAAAAATGA

CGATCGCTAA

AAGACCAGCA

AAAATTACTG

TGGCTTCTTC

GA.AAAATGCC

TTATCGTGAG

AGCCAAAAAA

GATTCACTTA

AGAACCTAAA

TGACTTAACA

TGAA.AAATTA

TTATGAGGAA

AAGCAGCTIG

ATAA

ATTGGCTATA

GCTTTTGTGA

GCCGACAAAA

TTGGGGTTAT

CAAATCATAC

CGTAATGATA

AAGTTACACT

AGTAAACATr

CA.AGAAACC

ATCGTTTCCG

TCGCAAAAAG

TTCTTTTAGG

TTCTGGTAGG

GCCATCAAAT

CTCCACAAGA

AATTGCAAGA

CTACGAAGGT

TAGCCAATCA

TAGAAATCGA

CACAAATCAT

ATGACTTAGA

CTGCAA CTGA

AGCCTTAATC

CTTTTTAGTA

GCCTTAC ETA AATTGATrT

AAACATGAAT

TTCTAAAGCT

CT'ITCTCTAT

ACAACACGAA

TGACCAATTG

CGATTTAGAT

GGAATCACCT

EF073-2 (SEQ ID NO:278)

S.

S

S

S

S

S.

S

S.

MKLNTKLLI GYILLGALI I KDKEAHYREL GLSPQEIDFF FKELVKEPKK LHLANHFLYT KLVKNDYEEI VSDDLDDLDV

AVAREYGFFA

RSTMSTAKKQ

HLPNIVDLTS

E-MS IAKSSLS

FVILVGFLVF

I IQLQENNNR

KHLEIEQHEV

QKAATEESPQ

VLYRKK<KNA.A

STKLRAIDLR

KN\KQTYEKLE

VNEIDQQ

DKSDQMPYLT

NDTTKVSKAL

ESAQI IDQLS EF073-3 (SEQ ID NO:279) CT ATCGAAAAAA GAAAAATGCC GCCGACAAAA GCGATCAA.AT GCCTTACTTA

ACGAAAGATA

TTCAGAAGTA

CGTTCAACTA

CTGTTTAAAG

ACACATTTAC

GTAAAAAACA

TCA.AAATTAG

CTCGAAATGT

AAGAAGCCCA

CAATCAGCAC

AA ITACGGGC

AG'ITAGTGAA

CAAATATCGT

AACAAACGTA

ITAAAAATGA

CGATCGCTAA

TTATCGTGAG

AGCCAAAAAA

GATI'GAC 1TA

AGAACCTAAA

TGACTTAACA

TGAAAAATTA

TTATGAGGAA

AAGCAGCTTG

TTGGGGTTAT

CAAATCATAC

CGTAATrGATA

AAGTTACACT

AGTAAACATT

GAAGAAAGCG

ATCGTTTCCG

TCGCAAAAAG

CTCCACAAGA

AATI'CCAAGA

CTACGAAGGT

TAGCCAATCA

TAGAAATCGA

CACAAATCAT

ATGACTTAGA

CTGCAACTGA

AA'FGTTT

AAACATGAAT

TTCTAAAGCT

CTTTCTCTAT

ACAACACGAA

TGACCAATTG

CGATTTAGAT

CGAATCACCT

CAAGTAAACG AAGACCAGCA AT EP073-4 (SEQ ID NO:280) YRKKKNAA DKSDQMPYLT KDKEA}{YREL GLSPQEIDFF FKELVKEPKK LHL.ANHFLYT KLVKNDYEEI VSDDLDDLDV

RSTMSTAKKQ

HLPNIVDLTS

EMS IAKSSLS I IQLQENMNR

KHLEIEQHEV

QKAATEESPQ

STKLRAIDLR

KNKQTYEKLE

VN~EDQQ

NDTTKVSKAL

ESAQI IDQLS EF0'74-1 (SEQ ID NO:281)

TAAAGGAGTT

ACCGTTGCAG

ATGGTAGATA

GGACGCGATG

GCCTACAATCG

TTCAAGCCTT

CAAGGTCGCG

GGCGATGAAC

GT'TAGACA

CTCAAAAAAT

TrcTTGCTG

TCTCTGGCAA

G'ITACAAACA

TCGTACCGGT

ATAACCAAAC

CAGT'ITTA T

AAGCCTTTGC

TCGACTTAGA

GAAGCTAAAA

GTTAACGCCT

AAAAGTGTTG

AGGAACATCA

TTCCTTCATG

CGACACTGCC

GGCACTTGGT

GAATGAAATC

GCA.ATTGGCG

AAAATAATTC

ACTCAACT

G=GCATAT

GCATCACTAA

AAAAGCGATC

TI'CCGACAAG

GGAGCAGATG

ATTCCTCAAG

ATTACTGCTG

cTGCTTrTCC

CTGCAGAMC

GGCATAACTG

ACCTTCAGA

GCACGACACG

AACTCGCACA

CACATATTCA

TGGAAMCATA

GCGACAACCA

CCTTCTTTCA

TGCGGATACG

GGCCTCAAAA

AGTAAATCAA

GA'ITCCTACG

ATTAAATAGT

ATTAGTCAAA

CGGCI'TGAT

AACCGTCATC

165 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

CCTGCTACGT

ACGATGGCAC

TCCCTAAATrC

GTCTGGGTTG

TTCCTCTATN

TGAAAATAGT CAAAGACCAT TATCGAGCAC AAGGAAAAAA TTTCATCATT CAGAATTCCC T'rA'rTAAAA CCTGGTGCCG CTTATGAAAC ATACATTACT GTTATTATGA TTACATTCC CCACAATTAT ATAACCAAGG CCGCCACGGT ATGAAGTTAT GACTFrGGGTT GCTCAAAGCA ACGATGCTCT AAAATACGAG

ATATI'

EF074-2 (SEQ. ID NO:282) MXLK( IIPAFPLLST VAVGLWLTPT QASADAADTM VDISGKKVLV GYWHNWASKG RDGYKQGTSA SLNLSZ-VNQA YNWVPVSFM< SOGTTRIPTF KPYNQTDTAF RQEVAQLNSQ GRAVLLALGG ADAHIQLVKG DEQAFANEII RQV? TYPFDG LOIDLEQL.AI TAGDNQTVIP ATLKIVKDHY RAQGKNFIIT MAPEFPYLKP GAAYETYITS LNGYYDYIAP QLYNQGGDGV WVDEVMTWVA QSNDALKYEF LYXI EF074-3 (SEQ ID NO:283) TGC TGCGCATACG ATGGTAGATA TCTCTGGCAA AAAAGTGTTG GGACGCGATG GTTACAAACA AGGAACATCA

GCCTACAATG

TTCAAGCCTT

CAAGGTCGCG

GGCGATGAAC

GGTTTAGACA

CCTGCTACGT

ACGATGGCAC

TCCCTAAATG

GTCTGGGTTG

TTCCTCT

TCGTACCGGT

ATAACCAAAC

CAGITI'TATT

AAGCCTTTGC

TCGACTI'AGA

TGAAAATAGT

CAGAATTCCC

GTTATTATGA

ATGAAGTTAT

TTCCTTCATG

GGACACTGCC

GCCACTTGGT

GAATGAAATC

GCAATTGGCG

CAAAGACCAT

TTATAAAA

TTACATTGCC

GACTTGGGTT

GTTGGATATT

GCATCACTAA

AAAAGCGATG

TTCCG3ACAAG

CQACCAGATC

ATTCGTCAAG

ATTIACTGCTG3

TATCGCCAC

CC TGGTG CCG

CCACAATTAT

GCTCAAAGCA

GGCATAACTG

ACCTTTCAGA

GCACGACACG

AAGTCGCACA

CACATATTCA

TGGAAACATA

GCGACAACC A

AAGGAAAAAA

CTTATGAAAC

ATAACCAAGG

ACGATGCTCT

GGCCTCAAAA

AGTAAATCAA

GATTCCTACG

A'ITAAATAGT

ATTAGTCAAA

CGGCTTTGAT

AACCGTCATC

TTTCATCATT

ATACATTACT

CGGCGACGGT

AAAATACGAG

EF074-4 (SEQ ID NO:284) AADTM VDISGKKVLV GYWHNWASKG RDGYKQGTSA SLNLSEVNQA YNVVPVSFMK SDGTTRIPrF KPYNQTDTAF GRAVLLALGG ADAHIQLVKG DEQAF'ANEII RQVETYGFDC LDIDLEQLAI ATLKIVKDHY RAQGKNFIIT MAPEFPYLKP CAAYETYITS LNGYYDYIAP WVDEVMWVA QSNDALKYEF LY

RQEVAQLINSQ

TAG DNQTVI P

QLYNQGGDGV

EF075-1 (SEQ ID NO:285) TAACCTATAA GAAAAAAATC GGGAAGAAAA ITT~lGC CAT GGAATGGGAG CAAAACTTTA CTAGAACGAT CTAAAAAAAC TTATI'AGGGA 'ITGATACAGG ATTGTTGCAA CAOTTAATCC ACCTATGTTG ATATrCCAGG GGTGGCGCAT CTTrAGCAAT TATGTTTCAA ITAATATGGC GTGAACAATA ATCTGACTTT TTGGATGGTG AACAAGCACT TACGGCCGCC AAGAACGTCA CTTAACAC- TAAGCAACTA GATTTAAGTT TTGATGACAT

ACAACCTGTG

TATCNTTGGA

TTGGGATGTT

TCAGGTCAAT

CGATGATGGG

TCGTGACAAG

TCAAGGAAAA

GGACACAGTT

TGGTTTAAAA

TTCTCAAGAC

CTCCTATTCA

AAGAAAAGTG

TCAAGAAATT

GAAAAAAATT

ATAAATTATT

ATTATCTTGG

TCTAAATC-AA

TTAAACAATA

CGTGTCGACC

CAAACAACCT

CAAGATAAAT

GAAAACTATT

GAATTAGTCA

GGATATGATT

AGAATGCGTT

ArrGAAGGCA

TTAACAGCTG

CC TAGATT

GGAGGNAAAA

NTCTATTTCT

TGGATAAAAC

AGGAGCCTTT

AAGGTCGTTC

TAGTCAGTCT

TGAATCACGC

TAAACATACC

ACGCGGTTGG

TTACGATTGG

ACGA.AGACCC

TCGTCCAAAA

TTTCTGATAA

ATCGCAGTGC

TATGTCAPAA

TCAGTTGTT

CTATGAAACA

TrcTGITI:TA

GGATACAACA

TGCTCGCGAT

CTATGCTTTT

TATTAATCAT

CGGAATCGAA

TAAAATCA

TAATGGTGAC

AGTCTTAAGT

TATGAAGACA

CTTTCGTAAA

166 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.juecalis Genes.

GTGAAACAAG ACCAACTTCA AGGTACTGGT ?TTATGCAAG ATGGTGTTC CT-ATCAACGT GTGGATGAAC AGAAI'AAC TCGTGTCCAA CAAGAG LTGA AAA.ATCAATT GAATACAAAA

TAA

EF075-2 (SEQ ID NO:286) MSKG KKIFAIIXGI ILXLFLAVVG MGAKLYWDVS KSMDKTYETV ERSKKSQVNL NNKEPFSVLL LG IDTGCDDGR VEQGRSDTI'I VATVNPRDKQ YVD IPGQGKQ DKLN-IAYAPG GAS tAMDTVE NYLNIP INHY VS INMAGLKE NNNLTFSQDG YDFTIGKISL DGEQALSYSR MRYEDPNGDY GRQERQRKVI NSVSNYQEIL TiAVSDNMKTD LSFDDMXKIA LDYRSAFGKV KQDQLQGTGF DEQELTRVQQ ELKNQLNTK EF075-3 (SEQ ID NO:287) ACTTTA TTGGGATGTT TCTAAATCAA TGGATAAAAC CTATGAAACA

TTLVSLARDT

LVNAVGG 1EV

EGIVQKVLSL

MQDGVSYQRV

A.

S S *5 9*

S.

S a..

S

a a.

GTAGAACGAT

T"TATAGGGA

ATTGTI'GCAA

ACCTATGTTG

GGTGGCGCAT

TATGTTTAA

GTGA.ACAATA

TTGGATGGTG

TACGGCCGCC

CTTAACAGCG

GATTI'AAGTI'

GTGAAACAAG

GTGGATGAAC

CTAAAAAAAG

ITGATACAGG

CAGTTAALTCC

ATATTCCAGG

CrI'AGCAAT

TTAATATGGC

ATCTGACTT

AACAAGCACT

AAGAACGTCA

TAAGCAACTA

TTGATGACAT

ACCAACTTCA

AAGAATTAAC

TCACGTCAAT

CGATGATGGG

TCGTGACAAG

TCAAGGAAAA

GGACACAGTT

TGGTI'TAAAA

TTCTCAAGAC

CTCCTATTCA

AAGAAAAGTG

TCAAGAAAT

GAAAAAAATT

AGG.TACTGGT

TCGTGTCCAA

TTAA.ACAATA

CGTGTCGAGC

CAAACAACCT

CAAGATAAAT

GAAAACTATT

GAATTAGTCA

GGATATGATT

AGAATGCGTr

ATTGAAGGCA

TTALACAGCTG

GccTTAGArr

TTTATGCAAG

CAAGAGFI'GA

AGGAGCCTTT

AAGGTCGTTC

TAGTC:AGTCT

TGAATCACGC

TAAACATACC

ACGCGGTTGG

TTACGAMG

ACGA-AGACCC

TCGTCCAAALA

=IICTGATAA

ATCCCAGTGC

ATGGTG=IC

AAAkATCAATT

TTCTGTTTTA

GGATACAACA

TGCTCGCGAT

CTATGCTTTT'

TATTAATCAT

CGGAATCGAA

TAAAATTTCA

TAATGGTGAC

AGTCTTAAGT

TATGAAGACA

CTI'GGTAAA

CTATCAACGT

GAATACAAAA

EFO075-4 (SEQ ID NO:288) KLY'.WDVS KSMDKTYE'IV ERSKKSQVNL NNKEPFSVLL LGIDTGDDGR YVDIPGQGKQ DKLNHAYAFG GASLAMDTVE NWNLTFSQDG YDFTIGKISL DGEQALSYSR NSVSNYQEIL TAVSDNMKTD LSFDDMKKIA DEQ)ELTRVQQ EL.KNQLNTK

VEQGRSDTTI

NYLNIPINHY

MRY'EDPNGDY

LDYRSAFGKV

VATVNPRDKQ

VS INMAGLKE

GRQERQRKVI

KQDQLQGTGF

TTLVSLARDT

LVNAVGGIEV

EGIVQKVLSL

MQDGVSYQRV

EF076-1 (SEQ ID NO:289) TAGAAAATAA CAGAGGAGCT AGCATI'GCTG CAGTITGCAAG AAGGTATCTC ATGTTTCCAA GGAAACCAAA AATTATTATC TTAAATGTTG TGGATCGTGT GTTAAAGACA ATACAGATTC AAGTTAAAAA AGTGGCCTAG

TAA

GAAGGAAATG

TGTCTCTCTI'

TCGTTATAAA

GAITGTCGAT

GAAACATGGC

TTTAAAAGAA

GCCATCT TI

AAAGCATCAA

GCAGTCATCC

GTTAAA;AGT

CATTTATCCG

GGTCAAAAT

CGCT~rTTCA

T'ITATAAAA

CAA1AAATTGG

CTTCTGAAAA

?rGTAGACGA

ATGATGAATT

TAGCTGAATA

CATTTATTGA

ATAATTCTTT

TATCGGTTTA

AATTATTAAG

TAAATTTGAT

AGATI'CTGTT

TGGCGAAAAA

AGATGCAATG

TTCAACA

TABLE 1. Nucleotide and Amino Acid Seqeuences: of E. faecalis Genes.

EF076-2 (SEQ ID NO:290) MK( ASTKIGIGLS IAAVASVSVA VIASEKIIKK VSHVSNRYXV KKFVDDKFOG NQKLLSTVDD LSDDELDSVL NVVD)RVKDGG; SKLAEYGEKV KDNTDSLKER FFTFIEDA,fl LXKWPRPSFF YKNNSFVST EF076-3 (SEQ ID NO:291) CATCG CTTCTGAAAA AAITATTAAG AAGGTATJCTC ATG ITCCAA TCGTTATAAA GTTAAAAAGT GGAAACCAAA AATTATTATC GATTGTCGAT-GAETTATCCG T'TGTAGACGA TAAATTTGAT ATGATGAATT AGATTCTGTT

'ITAAATC'ITG

GTTAAAGACA

AAGTTAAAAA

TGGATCGTGT

ATACAGATTC

AGTGGCCTAG

GAAAGATGGC

TTAAAAGAA

GCCATCTTT

GCI'CAAAAT

CGCTFTTCA

TTTTATAAAA

TAGCTCA-ATA

CAITTATTGA

ATAATTCTT

TGGCGAAAAA

AGATGCAATG

EF076-4 (SEQ ID NO:292) VIASEKIIKK VSHVSNRYKV KKFVDDKFDG NQKLLSIVDD LSODELDSVL NVVDRVKDGG LKKWPRPSFF YKNNS SKLAEYGEKV KDNTDSLKER FFTFIEDAMfl 9 EF077-1 (SEQ ID NO:293)

TAAT-GTAAAG

ACAATTATAA

GCGTATGGCA

ATTICAAACCT

GCAACCTTAG

GGTGATTCAT

GATAACTCGC

GAGGAAATCA

GGCTTGGTAA

CCAATTGAAA

TTGAAAATG

GTGAAAGAAT

CCTTTTACAC

ACACGTTTTG

ATTGCTTTAG

ACGATGGTCG

ACGCAAGGAC

GAATTAGTGG

ATTGTTGCTT

GTTTCTGGTG

AAT'TrCTGA

TCACGTAATG

AAAGAGACTA

GATCAAGAAT

GAATGITTAC

GTCATCATGG

ATTCCTATGG

AAAGATGACT

GCCAAACAAT

ACCGGGATCA

ATCT"TATCTG

.4*

S*

*9

TGAATGATGG

CAGGAGTTTT

TTATTTTAAT

TACGTGAAGG

CTGTGGGAGA

TAGAAGACTA

CACAAAAAGC

ATGITGGCGA

AAACCGGCAC

AAAATCCTGG

TTCCTGAAAA

CTCCGGCGCG

TAGTTGCCTA

CGGAACTCTI'

TGGCAGGGAT

AAAAATTAGC

AACTTTCTGT

GAI'CCCAGC

ATGCCAGAAA

CTGGCGTCAA

CACAAGAGTC

GCACATATTT

TGGAAAAATTI

CCGTTGCAGA

CACAAGATAA

TAGGAGATGG

CCTCATGG

TAAGTAAAGT

C GTATTAAT

TTCCGGCGCT

CTTTGCGTGC

GAGAGAAAAA

GGCATTATTA

AACAGGTTCT

AAAATATGGT

ATACTCCGCC

TGCCGCTGGA

TCATCGCTTG

TGAATTAGTA

ATCAACAGTC

GGATGAATTA

AACTGTAGCA

TCCAGCTCAT

CCTAATTGCA

AGTTGTTGCT

GGGTCGTTCA

TrCTGCAAAA

TGATCAAGTC

AAGCCTCGAA

GCAAGATGTC

GGCATFI'GTG

TCAAGAAACT

AGGCCGAA'T

ACACCAASTA

AACCATTIGCT

ATTAACTATT

TGTAAATGAT

AGCTACTGCG

CAGCCAACC

CGGAATT'TrT

AATCGCGGCT

TCGTCGAATT

GAGATGAAGC

TTITGAATrTA

GTAATGGCGT

GTCGATATTT

AC ETTGAT GA

AAAGCTAACC

AATGGCGAAA

GTAAAACCAG

GAGA-ATCTT

ATGTCGGGTT

GACAGTCAAT

TTTGTACGTI

GGTGTTGCTT

TCGCCGTGTC

AGTCGTCATG

ACGATTGCGT

CAACCAATCA

CAAGA.ATCAA

CCATTAAAA

GATGGTGCTG

GAAAAAATTG

AC TI=ACAG

CATCTTCAAC

GCAGAAGTAG

CTAA.AAGAAT

GCACCTTCGC

GCTAGTGAAA

GTCC4AATTG ATGTAACAAA AITGGGGATT 'TTTrACATCA CCCGAAITCG TAATGATGTT CTGGGAAATG TAGCGAT1'AC CGCTATCG7T 'TTrTAATTAT GTTGACTGGT AAGAGCTGA-A GTCATTATT1G ATTTAGAAGA TGTTTCTGTI' GGGAACTAGT TCCAGTTGAT CATTAACAGG AGAATCAAAA CCGTGAATGG TGACGGCTCT ATCAAACAAT TGTGAACTTA TAGCAGATCG CTATGCGGTA GGTTGTTTC AAAAAGTCCC C PTAATTCT ATCTGCCCCA GGGTCGTTAT TAAATCGGGA ITATAAA.AC AGGCACGATT ATGCTGGAAT AACTGCTGCT CTCATATI=TT AGCTAGATCA ATATACAGA TCTACCGGAA AGATACGGGT AGGTAAAAAG ATAAkAACGAC TATTCATATI' ACACTGTACG CCCAGAAGCA GAAT=IIAAT GCTGACGGGG GAATTACCGA AGTACATGGG TGCCTAAAGA AAATCATCCA TTGCTGCTGC AGACGTAGGT CTGCTGACGT TGTTAT'ITTA CCCAACATAC CATGAAAATT ATCTdcGTTT TAcTAA 1,,Ir ATGCTACAAG AAGTCGTGGA

GGCCAGTAA

IITGCTAGT

CACTGTGTCA

168 TABLE 1. Nucleotidc and Amino Acid Seqeuences of E. faecalis Genes.

EF077-2 (SEQ ID NO:294) MKHVTKLG IT

QTLREGKYGV

NSPQKAHRLN

IEKNPGDELM

FTLVAYLIAG

MVEKLASAKT

VAYARKQDVP

RNGTYLGR IT

CLPQDKLTIL

ODLSKVSQAV

LSALRARRIG

I ITGVLALLF

DILAITAIVA

GENLEDVSVE

SGSVNGDGSL

VAWFVSKSPT

IAFDKTCTIT

LKN ITDLAEV FTDTVR PEAK KELPKENH PV

EIAQDTMKIA

Q

EFILHQPNWA YGIILITGSV TLAVGEYWAS LMIL IMLTGG EINVGDELWV KPCELVPVDG KMVAEKTVAD SQYQTIVNLV RFAEVLVVAS PCPLILSAPI QGQLSVDQVQ PINAG ITAAE SGAGVKAFVD GAEIRVGKKN

ETMEKLHQLHLQRILMLTGD

IMVGDGVNDA PSLAAADVGI KQSVLIGIFI CVLLMLIAST MALMFtE4I

DSLEDYAAGI(

LVKTCTSTVD

KESAARPAHF

ALVAGMGRSS

LVGLAASVEQ

FVTQESQETE

QESVAETIAA

AMGAHGATAA

GI IPALIGAM

ANQELKSLLD

ESSLTGESKP

VRLADRYAVP

RHGVVIKSGT

2ESSH ILARS I

KIDKTTIHIS

EVGITEVHGE

SETADVVILK

LQEVJVDTVS I EF077-3 (SEQ ID NO:295) TCA GCCGAATTGG 0*

S

SS

0# 0 0

SO

50 5 50 00 OS S 050 0 5* 5 0 000

GCGTATGGCA

ATTCAAACCT

GCAACCTTAG

GGTGATTCAT

GATAACTCGC

GAGGAAATCA

GGCTTGGTAA

CCAATTGAAA

TTGAAAATGG

GTGAAAGAAT

CCTTTTACAC

ACACGTTTTG

ATTGCTTTAG

ACGATGGTCG

ACGCAAGGAC

GAATTAGTGG

ATTGTTOCTI'

GTTTCTGGTG

AATITI'GTGA

TCACGTAATG

AAAGAGACTA

GATCA.AGAAT

GAATCTITAC

GTCATCATG

ATTGCTATGG

AAAGATGACT

GCCAAACAAT

ACCGGGATCA

ATCTTATCTC

TTATTTTAAT

TACGTGAAGG

CTGTGGGAGA

TAGAAGACTA

CACAAAAAGC

ATGTTGGCGA

AALACCGGGAC

AAkAATCCTGG

TTGCTGAAAA

CTGCGGCCCG

TAGTTGCCTA

CGGAAGTCTT

TGGCAGGGAT

AAAAATTAC

AACTTTCTGT

GATTGGCAGC

ATGCCAGAALA

CTGGCGTGAA

CACAAGAGTC

GCACATATTT

TGGAAAAATT

CCGTTGCAGA

CACAAGATAA

TAGGAGATGG

GTGCTCATGG

TAAGTAAAGT

CTGTXFI'AAT

TTCCGGCGCT

CTTTGCGTGC

AACAGGTTCT GTAATGGCGT AAAATATGGT GTCGATATTT ATACTGGGCC AGTTTGATGA TGCCGCTGGA AAAGCTAACC TCATCGCTTG AATGGCGAAA TGAA'rTAGTA GTAAAACCAG ATCAACACTC GATGAATCTT GGATGAATTA ATGCTCGGGTT AACTGTAGCA GACAGTCAAT TCCAGCTCAT TTrGTACG TT CCTAATTGCA GGTGTTGCTT AGTTGTTGCT TCGCCGTGTC GGGTCGITCA AGTCGTCATG TTCTGCAAAA ACGATTGCGT TGATCAAGTC CAACCAATCA AAGCGTGGAA CAAGAATCAA GCAAGATGTC CCATTAAAAA GGCATI'=GTG GATGGTGCTG TCAAGAAACT GAAAAAATTG AGGCCGAATT ACTTTTACAG ACACCAATTA CATCTTCAAC AACGATTGCT GCAGAAGTAG ATTAACTATT CTAAAAGAAT TGTAAATGAT GCAC-CTI'CC AGCTACTGCG GCTAGTGAAA CAGCCAAGCG GTCGAAATTG CGGAA'TTI' ATCTGCGTTT AATCGGGGCT ATGCTACAAG TCGTCGAA ET GCCC 0000 5000 4 *405

S

40 0

SO

*0

TA.ATGATGTT

TAGCGATTAC

TTTTAATTAT

AAGAGCTGAA

A=TAGAAGA

GGGAACTAGT

CATTAACAGG

CCGTGAATGr-

ATCAAACAAT

TAGCAGATCG

GGTTCTTTC

CTTAATTCT

GGGTCGTTAT

TTGATAAAAC

ATG.CTGGAAT

GTCATATTI'

ATATTACAGA

AGATACGGGT

ATAAAACGAC

ACACTGTACG

GANIT=TAAT

GAATTACCGA

TGCCTAAAGA

TTGCTGCTGC

CTGCTGACGT

CCCAAGATAC

TACTAATGTT

AAGTCGTGGA

CTGGGAAATG

CGCTATCGTT

GTTGACTGGT

GTCATTATTG

TGTTTCTGTT

TCCAGTTGAT

AGAATCAAAA

TGACGGCTCT

TGTGAACTI'A

CTATGCGGTA

AAAAAGTCCG

ATCTGCCCCA

TAAATCGGGA

AGGCACGATT

AACTGCTGCT

AGCTAGATCA

TCTAGCGGAA

AGGTAAAAAG

TAITCATAT

CCCACAAGCA

GCTGACGGGG

AGTACATGGG

AAATCATCCA

AGACGTAGC-T

TGITATTTTA

CATGAAAATT

AATTGCTAGT

CACTGTGTCA

EF0~77-4 (SEQ ID NO:296) QPNWA YGIILITGSV MALMMFWEMI QTLREGKYGV DILAITAIVA TLAVGEYWAS NSPQKAHRLN GENLEDVSVE EINVGDELVV IEKNPGDELM SGSVNGDGSL KMVAEKTVAD LMILIMLTGG DSLEDYAAGK ANQELKSLLD KPGELVPVDG LVKTGTSTVD ESSLTGESKP SQYQTIVNLV KESAARPAHF VRLADRYAVP TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

FTLVAYLIAG

MVEKLASAKT

VAYARKQDVP

RNGTYLGRIT

CLPQDKLTIL

DDLSKVSQAV

LSALRARRIG

VAWFVSKSPT

IAFDKTGTIT

LKNITDLAEV

FTDTVRPEAK

KELPKENH PV EIAQDTMK IA

RFAEVLVVAS

QGQLSVDQVQ

SGAGVKAFVD

ETME(LHQLH

IMVGDGVNDA

KQSVLIGIFI

PCPLILSAPI

PINAG ITAAE GAE IRVGn(N

LQRILMLTGD

PSLAAAIOVCI

CVLLMLIAST

ALVACIAGRSS

LVGLAASVEQ

FVTQESQETE

QESVAETIAA

AMGAEGATAA

GIIPALIGAM

RHGVVIKSGT

ESSHILARSI

K IDKTT I HIS EVG ITEVHGE SETADVV ILK LQEVVDTVS I EF079-1 (SEQ ID NQ:297)

TAATICTAG

CCCAGGCTCT

ATCATTGATG

T'IT'IAGCG

AAAGCAAGCC

AACCAAGAAT

AAAACAACGA

ATTCCAAAAA

AAAGGAAGCT

GTCATTTCAG

AAAAAAGGCG

CAAATAAAAA

CTCGTCACTT

GGACATCGTA

CAACAAAATT

TTCATTATCT

CATCACCGAA

CATGCTTTAT

GTTTTATGAT

ATGCATTAAA

AAGAAAACAC

TAGCGAAAAA

AAAAACCAGA

TAAATGTCCG

CCTTGTTAGA

GCCATCGTGG

ATrGAATTTTA

CCGTTGAACC

TATTAACTTG

TCCCATATCA

TACTATTATG

GGTACAAGCG

GAAATTTTTA

TTIAAGGAG

TC=IFTACTG

TAACTATCTG

CAAAGAAATG

AGGCAGCAAT

CAA.ATCCTAT

TTTACCAATT

AGGAACCTCC

TCTCCCTCAA

TATCGAAGTIC

AACTGATACA

CACACCGTAT

ACCAGAAAAA

GACATTACT

ACGGAAAAAG

GAAAAACAAA

GAAGCAATGA

ATTATTGGAA

GATCAACAAA

GCTGAACTTC

CCTGGATTAG

TTTGAAAGTC

TTTGATAAAA

TATCCTACAG

GCCAAATTAT

AATGGGAAGA

AAAGATTTAC

ATGATA.AACA

GCAGCAGCGG

TAATTCGCCT

ACGACCAGAA

CAGCCT-GGGC CAATCACTGT AGTCAAAAAA GAAACGTCGT TAGGTGCATT TGCGTATCCT TI'ATCGCTCA TATCAAGCA A.AGAAAAAAT CGAAAAGAAA ATCCTTTTC TGAAACGICAA ATACGArI'GG TGTTTTAACC CGAATGCATT GCTATTGGAA GTGGTACGAA TACACATGCG TTACAGATT1' GCCAGAATTA CGCTTGCTTA TCAAGTAGAT ACATTGAGTC TGGCCAAGAT GTCATCGGTI' ATI'AG7TCGA GGATGAAAAA AGTGGCACAA GTGCGTTAAT TATTAGCGGC AACCAAAGTA G

SO

S

S@

0* S S *5

SS

S S

SO

06 @0 S 050 0 *5 5 0

S

S

0 EF079-2 (SEQ ID NO:298) MXSKKKRRI IDGFMIKLLI ICIGAFAYPF VSDALNNYLD QQIIAHYQAX ASQENTKEMA TTKKPDKSYF ESHTIGVLTI PKINVRLPIF ISGHRGLPQA KLFTDLPELK KGDEFYIEVN VTLLTCTPYM INSHRLLVRG HRIPYQPEKA IIWYKRRKKT TRKPK

ELQEKMEKKN

DKTI'JALLLEK

GKTLAYQVDQ

AAGMKKVAQQ

QELAKKGSNP

GSSLLECTSY

IK'IVEPTDTK

QNLLLWTLLL

GLDPFSETQK

PTGGTNTHAV

DLHIESGQDL

IACALIISCF

EF079-3 (SEQ ID N'O:299) see* 0000 0@ 0

TCCT

=TGTTAGCG

AAAGCAAGCC

AACCAAGAAT

AAAACAACGA

ATTCCAAAAA

AA.AGGAAGCT

GTCATTTCAG

AAAAAAGGCG

CAAATAAAAA

CTCGTCACTT

GGACATCGTA

CA.ACAAAATT

'ITCATTATCT

ATGCATI'AAA

AAGAAAACAC

TAGCGAAAAA

AAAAACCAGA

TAAATGTCCG

CCTTGTrAGA

CCCATCGTGG

ATGAATTTTA

CCGTTGAACC

TATTAACTTG

TCCCATATCA

TACTATTATG

GGTAC.AAGCG

TAACTATCTG

CAAAGAAATG

AGGCAGCAAT

CAAATCCTAT

TTTACCAATT

AGGAACCTCC

TCTCCCTCAA

TATCGAAGTC

AACTGATACA

CACACCGTAT

ACCAGAAAAA

GACATTACTT

ACGGAAAAAG

GATCAACAAA

GCTGAACTrC

CCTGGATTAG

TTTGAAAGTC

'TTTGATAAAA

TATCCTACAG

GCCAAATTAT

AATGGGAAGA

AAAGA'ITAC

ATGATA.AACA

GCAGCAGCGG

TTAATTGCCT

ACGACCAGAA

TATCGCTCA

AAGAAAAAAT

ATCCTTTT-TC

ATACGATTOG

CGAATGCAFI'

GTGGTACGAA

ITACAGATTT

CGCTIGCTTA

ACAT'GAGTC

GTCATCGGT1'

GGATGAAAAA

GTGCGTTAAT

AACCAA

TTATCAAGCA

GGAAAAGAAA

TGAAACGCAA

TGTTTTAACC

GCTATTGGAA

TACACATGCG

GCCAGAA ITA

TCA.AGTAGAT

TGGCCAAGAT

ATTAGTTCGA

AGTGGCACAA

TA'ITAGCGGC

EF079-4 (SEQ ID NO:300) 170 TABLE 1. Nucleotide and Amnino Acid Sequenices of E. faecalis Genes.

PF

VSDALNNYLD

TTKKPEDKSYF

I SGHRGLPQA

VTLLTCTPYM

I IWYKRRKKT QQIIAHYQAI( ASQENTKEMA ELQEIOIEYKK QELAKKGSNP

GLDPFSETQX

ESHTIGVLTI ?KINVRLPIF DKTNA[JLLEK GSSLLEGTSY PTGGTNTMAV KLFTDLPELK KGDEFYIEVN GKTLAYQVD)Q IKTVEPTDTK DLHIESGQDL INSHRLLVRG :4RIPYQPEKA AAGfrU(KAQQ QNLLLWTLLL

IACALIISGF

TRKP

EF080-1 (SEQ ID NO:301)

TACTTACACT

TTTATTAGGA

CTTACCTTTG

CAATTACTGA

AAAACAAAAG

GAATATGCAG

CTCATTGATA

ATGACAGATG

AATTATTTCG

GTTCCTGGG

ATTACCCCGC

TTAGGCATTA

GAAAAAACAA

ACGCGTCCTA

ACCCACTCAA

CGTT'TAGGGC

GGTTATTTCC

GCC7rGCCCT

CACCTGATCA

CCTCTGTCTT

ATCATTATCT

TGGACTTACG

CACGAATIGA

CGGCTGCTCA

GCCACTATCG

TGGAAATGGT

ATAT'ITCTAA

CTTTAAAC'T

TTCCTAAAAA

CTGGCGGCGG

TAGCAACGT1T AGGCAT1TTTC

TATGAAAAAA

ACCCCTI'TCG

AATCAACCAA

TATTGTAACC

TTTAAATAAA

GAAAATCTAC

TGATACCTTA

AAccTTTGTT

TGTGGACAGC

AATTGCTTFI

ATATCAATrA

AACCTCCCGC

CAATGGCGGC

CACATTCGGT

CGACTITTAC

GCGGCTGAAA

cTAAAGCAAG

ACAAATAATA

GTTGGCAACG

ATCTCTACTT

GATAAAATAT

CAGGAAACTC

GAAACAGGTA

GCTGCTGCGC

AAATTTCAA

ACAGATCAGT

AGTGGCGGAA

GGCGGCGGTC

GCTCGACTICT

CTAMTI=r

ATTCAATTGA

AdATACAACC

ATACCTATGG

ACCAAAATGC

CTGGAAkACAT

GGGATAATAT

AAGCATTTGT

AAATCACTCG

TGATACTCAG

GTTATCAATA

TAACCAACTC

TGGGCGGTGG

GAAGTTTTTA

TAGCACTCTT

CCTAATACTT

TGATGGCGCA

TrTTAGAAGAA

CGATGALACAA

GATTCTTTTT

GATTGATTAT

GAGTCAAGGA

TAATAAAGGG

TI'ATAAAGTC

TITCGTCTTC

TCCCTTTAGG

TTTCATCACT

TGGTAGCACC

EFOSO-2 (SEQ ID NO:302) MK(KR LLPIFFLILL TFGL.ALPVSA

AENSIDDG-AQ

LLTPDQINQL KQEIQPLEEI( TKASVFIVTT

NNN'TYGDEQE

IDMDLRKIYI STSGNMIDYN TDARIDDTLD

KIWDNMSQGN

PGGHYRVDSE TGKITRYKVI TPLEMVIAFA AALILSLVFL KTTLNLTSRT DQLTNSFI IT RRIPKNNGGS

GGMGGGGST

YADHYLLNKV

YFAAAQTFVQ

GINI SKYQLK

HSTGGGTFG

GKDQNAILFL

ETQAFVNKGV

FSSYQYPFRE

CCRSF

EF080-3 (SEQ ID NO:303) GGCTGAAA ATTCAAT GA TGATGGCGCA

CAATI'ACTGA

AAAACAAAAG

CAATATGCAG

CTCATTGATA

ATGACAGATG

AATTATITCG

GTI'CCTGGGG

ATTACCCCGC

TrTAGGCATTA

GAAAAAACAA

ACGCGTCGTA

ACCCACTCAA

CACCTGATCA

CCTCTGTCT

ATCATTATCT

TGGACTTACG

CACGAATTGA

CGGCTGCTCA

GGCACTATCG

TGcAAATCGT

ATATTTCTAA

CTTTAAACTT

'FrCCTAAAAA

CTGGCGGCGG

AATCAACCAA

TATTGTAACC

TTAAATAAA

GAAAATCTAC

TGATACCTTA

AACCTTIGTT

TGTGGACAGC

AATTGCTTTT

ATATCAATTA

AACCTCCCGC

CAATGCGCC

CAr-A ICGGT

CTAAAGCA-AG

ACAAATAATA

GTTGGCAAGG

ATCTCTACT

GATAAAATAT

CAGGAALACTC

CAAACAGGTA

GCTGCTGCGC

AAATT7'rCAA

ACAGATCAGT

AGTGC.CGGAA

GGCGGCGGTC

AGATACAACC

ATACCTATGG

ACCAAAATGC

CTGGAAACAT

GGGATAATAT

AAGCATTrT

AAATCACTCG

TGATACTCAG

GTrATCAATA

TAACCAACTC

TGGGCGG;TGG

GAAGT

TTTAGA.AGAA

CCATGAACAA

GATCTTTTT

GATTGATTAT

GAGTCAAGGA

TAATAAAGGG

TTATAAAGTC

TTTGGTCTTC

TCCCT'ITAGG-

TTTcATcACT

TGGTAGCACC

EF080-4 (SEQ ID NO:304)

AENSIDDGAQ

LLTPDQINQL KQEIQPLEEX TKASVFIV T NNNTYGDEQE YADHYLLNKV GKD)QNAILFL 171 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. [aecalis Genes.

IDMDLRKIYI

PGGHYRVDSE

KF1'LNLTSRT STSGNMI DYM

TGKITRYKVI

DQLTNSFITT

TDARIDTLD

TPLEMVIAFA

RRI PKNNGGS K IWDNMSQGN

AALILSLVFL

GGMGGGGS ET YFAAAQrTFVQ

GINISKYQLX

H STGGGTFGG

ETQAFVNKGV

FSSYQYPFRE

GGRS

EF081-1 (SEQ ID NO:305)

TGAATGGAAC

TCTGCCTTG

GCCAAACAAG

AA.ACAGGAAT

TATGATACTA

GGAGTCAAAG

TATAAATTAT

ATCGCCAAAA

ATGTCAGGAA

GATCGTAATG

GGCAAACTCG

GCGTTTCTGC

TTTGTACCAA

AAAGATACAG

GAAGCAATCG

'ITCTAATCGC

AATT1AGCCGA

TTGATAAGTT

AATCTGTTGT

CTAGTGATGT

CGATGAGCAC

AAGGCGATAC

ATGATAAAAT

GTAGTGGGCT

GTTCTGCC

TTGATGAAAT

TCACAGTCGC

AGTCACGFI'A

TAATAAAAAA

TGCTGCCGGT

AGcGAAGAAA

ACCGTCCGTT

TGAAAAATAC

TCAAGTCAAA

CCTTTAGGC

CTACCAAATC

TTCGATI'CAA

AGCAATTAAC

AGA-AAAAACA

CAATCAAAAG

TTCTGAACCA

TATCCGCTG

TCTTCAAAAA AACCACTrAT TCI'GGTGTT GGCGGGTATT ATGCTTATAG TCAATGGCAA ACAGCTACTA CATTTTTAAA CGTATTGTCA GTTCAAGAAG CTAGC~rAAA GAAAAAT'-GC C-AAGCAATI AI'CAGGGAT TCAAGCAGAA AAGGCGAAAG ACAATCAATA CACATTTACC GAAATGAAAG ATTTGTCTTA TCA.ATCAAGT GCTTGGAAGC CATCTTTAAT

TTTTCCAGAT

GTAGATAATG CCA.AACCTGG AGAAATTGTC AA.AGTGTTTC ACGAAGTGGG CGTAGTGCCT GCCAATATCA AAGCTTTTAG

TGATAAATTC

TTAAGCCAAG GATGCGTCCA

AGCAGACTCC

GTGACAGAAT TACCAACAGG

GGCTGCGACA

GGGGAAGCAN TGCGCKATTA A EF081-2 (SEQ ID NO:306)

MERSNRNKKS

QEFDKLPSVV

KLSMSTPLGE

RNGSGLAINK

VPITVASEPV

SKKPLILGVS

QE-ASLKKNGY

MKDLSYQSS I

V'FDEVGVV'PG

TELPTGAATK

AL.VLIAAAGG GYYAYSQWQA KQEL.AFAKKT DTKSVVEKYQ AIYSGIQAEG VKASDVQVKK

ATTFLNVLSK

AKDNQYTFTY

DNAKRGEIVD

SQGWVQADSF

AKKGDTYQ IA

KLGSGAEKTA

DTESRYYPLG

WKPSLIFPDM

NIKAFSDKFG

EAXRN

VSVDEINQKL

a.

a.

a a a a a a a a a a EF081-3 (SEQ ID NO:307) T GGCGGGTATT ATGCTTATAG TCAATGGCAA

GCCAAACAAG

AAACAGGAAT

TATGATACTA

GGAGTCAAAG

TATAAATI'AT

ATCGCCAAAA

ATGTCAGGAA

GATCGTAATG

GGCAAACTCG

GGCGTTTCTG

TTTGTACCAA

AAAGATACAG

AATTAGCCGA

TTCGATAAGTT

AATCTGITGT

CTAGTGATGT

CGATGAGCAC

AAGGCGATAC

ATGATAAAAT

GTAGTGGGCT

GTTCTGGCGC

TTGATGAAAT

TCACAGTCGC

AGTCACGTTA

ACCAAGAAA

ACCGTCCGTT

TGAAAAATAC

TCAAGTCAAA

GCCTTTAGGC

CTACCAAATC

TTCGATI'CAA

AGCAATTAAC

AGAAAAAACA

CAATCAAAAG

TTCTGAACCA

TTATCCGCTG

ACAGCTACTA

GTCAAGAAG

CAAGCAATT

AAGGCGAAAG

GAAATGAAAG

GCTTGGAAC

GTAGATAATG

AAAGTGTTTG

GCCAATATCA

TrAACCCAAG

GTGACAGAAT

GGGG

CAT1TITAAA CGTATTGTCA CTAGCT1'AAA GAAAAATGC ATTCAGGGAT TCAAGCAGAA ACAATCAATA

CACATTTACC

A'TTG.TCTTA

TCAATCAAGT

CATCTMTAAT

TTTTCCAGAT

CCAAACGTGG AGAAATTGTC ACGAAGTGGG

CGTAGTGCCT

AAGCTTTTAG TGATAAATTC GATGGGTCCA

AGCAGACTCC

TACCAACAGG

GGCTGCGACA

EFO81-4 (SEQ ID NO:308) G GYYAYSQWQA KQELAEAXK<T

ATTFLNVLSK

QEFDKILPSVV QE-ASLKKNGY DTKSVVEXYQ AIYSGIQAEG vKAsDVQVKK

AKDNQYTFTY

KLSMSTPLGE MKDLSYQSSI AIKKGDTYQIA

WKPSL

1 IFPDM SGNDKISIQV DNAXRGEIVD RNGSGLAINK VFDEVGVVPG KLGSGAEKTA NIKAFSDKFG VSVDE-INQKL

SQGWVQADSF

VPITVASEPV TELPTGAATK

DTESRYYPLG

172 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

EF082-1 (SEQ ID NO:309)

TAAAAAATGA

CTTTTAAATA

AITTACG=TG

CCTGTrGACAC

GACTATGTTT

TCGGCACAAC

GTTCAAGTAA

GCGCAATTTG

TTAGCAGCAA

ACCTTGGATC

ATCCGCACTT

ACTGTTCCTG

ATT'ITGGATG

AAAAGATCGT

GTTCAAAAGT

CTCTTGATAA

CTA.ATCCTGC

CAAATATCCA

TGGATCAAGT

CTGACA.AACG

CTACAAGTGA

CACCCAA'ITC

CAACTCGTC

GGACATT1AGC

CGACAACGAA

ATACACCACT

GCGCATTCA

TGA.AGCAGCT

TACTGTCACG

TGATCCTCAT

TTTTGGATCA

GCAAAATAGT

TCGTCTAAAT

TTCA-ACACCC

AACACAGTTA

CGCCAC'ITCT

T'rFTGGTAGC

AAAAGTTGCA

'FrAA

AGCAT=~GT

CAAGTCGCTT

CCACCTGTCA

CAACCTGGTA

AAACAAATTC:

ACTCCCGATT

CTTGGCTGGA

GCTGTTTGG

CI'CTTTGG

CCTGTGGCGA

GGANCGACCG

GCTAAACAAT

TCGTTGCTAC

CTATTCAATC

ACCCGACGAA

CAGCCGGACC

AAGCCGGAAC

TAATTAGCCT

AATTATCAGT

ATAATGCATC

CGCCATTAAC

CTGCCCCTCT

CTGCTCAAGG

AtAAALACAAC

GCCTCTTATG

CAACGCTGAT

CCCTTCTCAC

CCTTAGTA'-

AGCGATCTAT

GCCAAACTAT

TAAACAGAGT

CTTGACCTTT

GGTCCCAGTA

TTCAACAGGA

CATTCAATTA

GCTTACTTG

EF082-2 (SEQ ID NO:310) HI(KIVRISS ILFVATPLML LNSSKVEAAQ VASIQS4ADI TFALIDNTVTP PVNPTNPSQP VTPNPADPHQ PGTAGPLSID YVSNIHFGSK QIQAGTAIYS AQLD)QVQNST GDLISVPNYV QVTDKRGL 'NL GWKLSVKQSA QFATSDSTPA VLDNASLTFL AATPNSTQLL SLAPLTVPVT LDPTGAATSP VATAALSTGM GTWTL.AFGSG XTAAQGIQLT VPATTKKVAA KQYKTTLTWI

LDDTPL

EF082-3 (SEQ ID NO:311) AGCT CAAGTCGCTT CTATTCAATC CAACGCTGAT e.

t

ATTACGTTIG

CCTGTGACAC

GACTATGTTT

TCGGCACAAC

GTTCAAGTAA

GCGCAATTTG

TTAGCAGCAA

ACCTTCCATC

ATGGGCACTT

ACTGTTCCTG

ATTTGGATG

CTC'ITGATAA

CTAATCCTGC

CAAATATCCA

TGGATCAAGT

CTGA. AACG

CTACA.AGTGA

CACCCAATTC

CAACTGGTGC

GGACATTAGC

CGACAACGAA

ATACACCACT

TACTGTCACG

TGATCCTCAT

=ITGGATCA

GCAAAATAGT

TGGTCTAAAT

F1'CAACACCC

AACACACTTA

CGCCACTTCT

T'ITGGTAGC

AAAAGTT1GCA

CCACCTGTCA

CAACCTGGTA

AAACAAATrC ACTGGCGA T

CTTGGCTGGA

GCTGTTTTGG

CTTTICTTT'GG

CCMTGGCGA

GGANCGACCG

GCTAAACAAT

ACCCGACGA-A

CAGCCGGACC

AAGCCGGAAC

TAATTACCT

AATTIATCAGT

ATAATGCATC

CGCCATTA-AC

CTGCCGCTCT

CTGCTCAAGG

ATAAAACAAC

CCCTTCTCAG

CCTTAGTAT'

AGCGATCTAT

CCCAAACTAT

TAAACAGAGT

CTTGACCTTT

GTCCCAGTA

TTCAACAGGA

CATTCAATITA

GCTTACTTGG

EFOB82-4 (SEQ ID NO:312) AQ VASIQSNADI TFALONTVTP PVNPTNPSQP VTPNPADPHQ PGTAGPLSID YVSNIHFGSI( QIQAGTAIYS AQLDQVQNST GDLISVPNYV QVTDKRGLNL CWKLSVXQSA QFATSDSTPA VLDNTASLTFL AATPNSTQLL SLAPLTVPVT LDPTGAATS P VATAALSTGM GTWTLAFGSG XTAAQGIQLT VPATTKKVAA KQYKTTLTW I

LDDTP

EF083-1 (SEQ ID NO:313)

TAATTTAAA-A

GCTCTGTTTT

GCGATTTATC

CAGAITGCAG

GCAAGTGCTA

GACA.AGGAGA

TTATGCCTAC

AAGGTGAACA

GCTACAATC

1 FGCCCAAGG

AATAAAAATG-

AGCTATG=T

AGGTCGCTI'

TAGCCGTATT

TAAACGTGCG

AAAAAGAAAA

GCCGCAAAAG

GGCTATGCAC

TATGAACAAT

CATACCTATA

TTTTAGCACC

GAGACCAAGG

ATGATAAAT

ACACATATA.A

T=TGGTATGA

AGCTTGTC

TTCCGATIGG

CGCTATTGCC

AACGCAAGTG

CAcTTGGGGA TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes

AACATGGACA

AATTCCATCG

GGAGGATATG

ATGCGCAGAA

CTAAATCATG

GCGTATCCTA

GGTATTGGTA

GAT'ITAACAG

CCAGCAACAG

GATACCGTCA

TGGGTAAAAG

GGTATCTTGT

CCTGATAAGC

GCTTATCAAT

AATCTTATTT

ACGGTTAAAT

GCTTTAGCTG

AATTAI'AA

ITTGCGAAAAC

TTGcATTAGA

TAAGTTCAGA

TCAAACAGGC

TAAACTATCA

TCGATGGTGT

ITTTGGCAAT

GAATTACGGA

ATGCAGGCGA

AAGTGAAATT

GAAACAGCTA

CATGGATTAG

AACCAGAAGC

ATGGAACAGA

ATCCTGGTCA

ACGGCGATAA

CATTAAACGG

AACAATGGAT

TACTTTM'TGC

TrTTGAACAT GGAGCGTTrGO TGCCGAAAAA

GCAGCAAATA

TCGCTATACT ccAATG.TATT ACAAATCATC

AAACAGTTTC

GTCACCATAT

CCATTGTATG

CACATCCGCT TATA MGCAG TAGTGG'ITAT

ACAGATACCA

ACGAAATTGAA AAAATACCTA TAATCTAGAT

GCTTGGCAA

CAAAGTGCAAGAACTAACT

CAAAGGTGAT

ATTI'AATTAT

GACTCATGTG

GTACAATACG

CTATCAAACG

TTGGCGGCAT

AGTTTTGAAA

GTCAATGGAT

TTTATCTAGT

ATTGCAGCAA

ATTAGcAAAT

CCTAACTI'A

CACGTATTCA

CTAGTGTTCC

CAGAGACAAT

ACAGCTATAA

CTAACTC 11T

CTTATTTCCC

GTGGTTTAGA

ATAAACCAGA

ATTCTGATGT

CTGGGGAAGC

GAAGCAGAGT

TGCCAGACGC

GAGAPLACATT

TAAATGCATT

CGGCAACAAG

AACTTGGCAC

TTTATCCAGC

A-ACGCCTAAA

AGATGGATAT

TTTGTACGGT

GCCATTTACA

ATGGATTGCT

A.AGCATGGAT

TGGTAACGTA

AACGGATACG

TAAAGTTGGC

TATTCCGCAA

ATTGCTTGAA

A.ACAGTCGTC

ATCAAGTAT

GGCTAATCCA

TAATGTCTAC

TACTTATCAA

TCAAACATTG

EF083-2 (SEQ ID NO:314) MK KKILAGALVA LFFMPTAMFA AKGDQGVDWA IYQGEQGRFG

YAHDKFAIAQ

IGGYNASGIY EQYTYKTQVA SAIAQGKRAH TYIWYDTWGN N4DIAKTTMDY

FLPRIQTPKN

SIVALDFEHG ALASVPDGYG GYVSSDAIEKA ANTETILYGM RRIKQAGYTP

MYYSYKPFTL

NHVNYQQIIK EFPNSLWIAA YPIDGVSPYP LYAYFPSMDG IGIWQFTSAY

IAGGLDGNVD

LTGITDSGYT DTNKPETDTP ATDAGEEIEK IPNSDVKVGD TVKVKFNVDA

WATOEAIPQW

VKGNSYKVQE VTGSRVLLEG ILSWISKGDI ELLPDATVVP D)KQPE-ATHVV

QYGETLSSIA

YQYGTDYQTL AALNGLANPN LIYPGQVLKV NGSATSNVYT VKYCDNLSSI

AAKLG'LTYQA

LAALNGLANP NLIYPGQTLN

Y

4*

SSSSSS

S

S

555.

5555

S

5555 *555

S.

EF083-3 (SEQ ID NO:31S) AAAAG GAGACCAAGG TGTGGATTGG

GCGATTTATC

CAGATTGGAG

GCALAGTGCTA

AACATGGACA

AATTCCATCG

GGAGGATATG

-ATGCGCAGAA

CTAAATCATG

GCGTATCCTA

GGTATTGGTA

GAITTAACAG

CCAGCAACAG

GATACCGTCA

TGGGTAAAAG

GGTATCT'TGT

CCTGATAAGC

GcTTATCAAT AATCTTATm ACGG ETAAA'I

GCTTTAGCTC

AAT

AAGGTGAACA

GCTACAATG-C

TTGCCCAAGG

TTGCGAAAAC

TTGCA'ITAGA

TAAGTTCAGA

TCAAACAGGC

TAAACTATCA

TCGATGGTC.T

TTTGGcAATT

GAATTACGGA

ATGCAGGCCA

AAGTGAA6ATT

GAAACAGCTA

CATGGATTAG

AACCAGAAGC

ATG.GAACAGA

ATCCTGTCA

AcGGcGATAA

CATTAAACGC

AGGTCGCTTT

TAGCGGTATT

TAAACGTGCG

AAcAATGGAT

TTTTGAACAT

TGCCGAAAAA

TGGCTATACT

ACAAATCATC

GTCACCATAT

CACATCCGCT

TAGTGGTTAT

AGAAATTGAA

TAATGTAGAT

CAAAGTGCAA

CAAAGGTGAT

GACTCATGTG

CTATCAAACG

AGT'ITTGAAP

TTTATCTAG1

ATAGCAAAI

GGCTATGCAC

TATGAACAAT

CATACCTATA

TACTTTTTGC

GGAGCCC LrGG GcAG~cAAATA

CCAATGTATT

AAAGAGTTTC

CCA'TGTATG

TATATTGCAG

ACAGATACCA

AAAATACCTA

GCTGGGCAA

GAAGTAACTG

ATTGAATTAT

GTACAATACG

TTGGCGGCAI

GTCAATGGA'I

ATTGCAGCAP

CCTAACTTGP

ATGATAALAT C

ACACATATAA

TTI'GGTATGA

C

CACGTATTCA2 cTAGTGTCC

CAGAGACAAT

ACAGCTATAA

CTAACTCTTT

CTTATTTCC

C

GTGGTTTAGA

ATAAACCAGA

ATTCTGATGT

CTGGGGAAGC

GAAGCAGAGT

TGCCAGACGC

GAGAAACATT

TAAATGGATT

CGGCAACAAG

AACTTGGCAC

TT1'ATCCAGG

:GCTATTGCC

AcGCAAGTC :ACTTGGcA k.AcGCCTAAA kGATGGATAT rTTGTACGGT GccATTTACA

ATGGATTGCT

AAGCATGGAT

TGGTAACGTA

AACGGATACG

TAAAGTTGGC

TATTCcGCAA ATTGCTT1GAA

AACAGTCGTC

ATCAAGTAT

GGCTAATCCA

TAATGTCTAC

TACTTATCAA

TCAAACATTG

TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

EF083-4 (SEQ ID NO:316) KGDQGVDWA IYQGEQGRFG

YAHDKFAIAQ

ICGYNASGIY EQYTYKTQVA

SAIAQGKRAH

SIVALDFEHG ALASVPDGYG

GYVSSDAEKA

NHVNYQQIIK EFPNSLWIAA

YPIDGVSPYP

LTGITDSGYT DTNXPETDTP

ATDAGEEIEX

VKGNSYKVQE VTGSRVLLEG

ILSWISKGDI

YQYGTDYQTL AALNGLANPN

LIYPGQVLKV

LAALNGLANP*NLIYPGQTLN

TYIJDTWGN

ANTETILYGM

LYAYFPSMECG

I PNSDVKVGD

ELLPDATVVP

NGSATsNVYT MD IAKTTIMDY

RRIXQAGYTP

I GIWQFrSAY

TVKVKFNVDA

DKQPEATHVV

VKYGE)NLSSI

FLPRIQTPKN

MYYSYXPFTL

IAGGL.DGNVD

WATGE-AIPQW

QYGETLSS IA

AAJ(LGTTYQA

EF084-1 (SEQ ID NO:31 7 TAGTCAAACG TTTATTTITTT CCT1'AAATCC CTATTGAATT GGAGGAGAAC TATGA6AGAA6A AGCGGTATGA TGGCCACCAT

TGCGGCCGCC

AAAAATCGCC GTGTTGGGAA

AAAATTATA

AATAATCGGC CCGCAGAAGA

AATCATTTCA

AGCGCATTI' CACAAT'I'IGA

TAACTATGAT

CACTTAAAAG AAGAAGATCA

CGGACGCATG

GTTGATGCGC TATTTA6ACCG

CATI'AACGA

GTCACAAAAT TACTACGAAA

AGACGATCAA

ATTTATCCAC CGTGTGTTGT

ATTAACAACT

ACTGGTGATG -GCTATAAACT

AGCCAAAAAA

ACCGAATCAC CTATTATTTC

TGAAGAACCT

TCTTTACA6AG ATGTTAATTT

AACTGTTTTG

CAAATGGATA TGCTGTTTAC ACATT' ITGC .AGTTTTAITA ACCAAGAATT

AACTCGCAAC

**GTCTTTCCGA CAAAATCTI'T

TGAAGAAGTG

*CTTTCCTTTG TGGAACTACT

GAAAGACTTT

GAAAAATC'IT TTGTCACAGG

CGGTGGAT'I

*GAGAGCAAAT TAGTCAATGG

-TATTTTTI

*ACTGCAGGCT ACAATGTTAC AGCTGCATT1 GCAGAAATTG CAGAATACAC

CTATTTACCI

EF084-2 (SEQ ID NO:318)

AGAAAAAATC

TTTGATGTA6A

GAAGCAGGCG

ATGACTCGTG

TTTATTCCTG

ATCATGAACT

TTCCCTGTI'A

TTAGGAGTCA

ATAATTGGCG

GGCGGCCGCA

ATGGGGCATA

'rTrATCCTGG AAccAAAAAG

ATTTCAGGAC

GGTAATCAAC

CCTGCCAAAC

CAGTTCACTC-

TCCCTCAAAC

GCTGOGTGAAC

GTCACTGGAC

SAT1'GAAGAAC CCGTAA6TTAT G

TI'ATTGTCGG

CTCAAGTATT

GCGGCCGCTG

GGAATGGAAA

Tr'IrrGAATC

CAGATAAATC

CTGT'T1TAC

TTGAAACCGA

CTTATCCTTC

CCATCAGCCC

ATAAAACGTT

GAAAACCTI'

CTGCCGCGCT

CTGTCACGGT

*AACTAACAGA

*TTACGAAAAC

AAGTGACCCC

-TTTAGATAT

-ATGTIMCTGG

TCTAA

;GTACACTAC

EGCTGGGACG

kTTGATTGAA

L'AATGTAACC

kTTr-TTTATAC

CA.ATGGTATT

GAAGTCAATTr

AAAAACACAG

ACTGGAAAAA

CACAGGAGCA

GCTCTACCCT

GCAAGGTCTC

AGTTAATCAT

CCGCTGTT-CT

AGCCTTGGAT

AAAGCAACGN

ATTGCCTTTG

TAAAACAATG

TAATGGCTAT

CTCCCATGCC

4*t*

C

MKKl'F DVIIVGAGTS GmmATIAAAE

AGAQVLLIEK

NRRVGKKLLM TGGGRCNVTN NRPAEEIISF

IPGNIGYFLYS

LKEEDHGRMF PVTDKSKSIV DALFNRINEL

GVTVFTKTQV

YAPCVVLTTG GRTYPSTCAT GDGYKLAKKM

GHTISPEJYPT

LQDVNLTVLN QKGKPLVNHQ MDMLFTHFGI SGPAALRCS S FPTKSFEEVP AKQLTEKQRL SFVELLKDFQ

FTVTKTLPLE

SXLV-NGLFFA GELLDINGYT GGYNVTAAEV

TGHVAGSHAA

AFSQFONYDI

TKLLRI(DDQI

ESPIISEEPF

FINQELTRNG

KSFVTGGGIS

EIAEYTYLPI

MNFFESNGIH

IGVETELEKI

ILDKTLQGLS

NQPvTVALDv

LKEVTPKTME

EEV

EF084-3 (SEQ ID N~O:319) a C. C GAAGCAGGCG CTCAACTATI' ATTGAF1'GAA AAAAATCGCC GTGTTGGGAA AAAATTATTA

ATGACTGCTG

AATAATCGGC CCGCAGAAGA AATCATTI'CA TrATTCCTG AGCGCATTTT CACAATTGA TAACTATGAT

ATCATGAACT

CACTTAAAAG AAGAAGATCA CGGACGCATG TCCCTrGTA GTTGATGCGC TATTTAACCG CATTAACGAA

TTAGGAGTCA

GCGGCCGCTG

GGAATGGAAA

TTTTTGAATC

CAGATAAATC

CTGTTT=AC

TAATGTAACC

ATTTTTATAC

CAATGGTATT

GAAGTCAATT

AAAAACACAG

175 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

GTCACAAAAT

ATTTATGCAC

ACTGGTGATG

ACCGAATCAC

TCTTTACAAG

CAAATGGATA

AGTTTATTA

GTGTTTCCGA

C=TCCTTTG

GAAAAATCTT

GAGAGCAAAT

ACTGGAGGCT

GCAGAAATTG

TACTACGAAA

CGTGTGTTGT

GCTATAAACT

CTATI'ATTTC

ATOTTAATT

TGCTGTTTAC

ACCAAGAATT

cAAAATCTT

TGGAACTACT

TTGTCACAGG

TAGTCAATGG

ACAATGTTAC

CAGAATACAC

AGACGATCAA ATAAT1CGCG ATTAACAACT

GGCGGCCGCA

AGCCAAAAAA ATGGGGCATA TGAAGAACCT TTI'ATCCTGG AACTGTTTTC AACCAAAAAG ACX1Tr-TGGC ATTCAGGAC AACTCGCAAC GGTAATCAAC TGAAGAAGTG

CCTGCCAAAC

GAAAGACTTT CAGTTCACTG CGGTGGGATT TCCCTCAAAG TTATTTTT-. GCTGGTGAAC AGCTGCATTT

GTCACTGGAC

CTATTTACCA ATTGAAGAAG

TTGAAACCGA

CTTATCCTTC

CCATCACCCC

ATAAA.ACGTT

GAAAACCTFI'

CTGCCGCGCT

CTCTCACGGT

A.ACTAACAGA

TTACGAAAAC

AAGTGAC C

FAGATAT

ATGTTGCTGG

TC

ACTGGAAAAA

CACAGGAGCA

GCTCTACCCT

GCALAGGTCTC

AGT1'AATCAT

CCGCTGTTCT

AGCCTTGGAT

AAAGCAACGN

ATTGCCTTTG

TAAAACAATC

TAATGGCTAT

CTCCCATGCC

EF084-4 (SEQ ID NO:320) E AGAQVLLIEX NRRVGKKLLM TGGGRCNVTN LKEEDHGRMF PVTDKSKS IV YAPCVVLTTG GRTYPSTGAT LQDVNLTVLN QKGKPLVNHQ FPTKSFEEVP AKQLTEKQRL SKLVNGLFFA GELLDINGYT NRPAEEI 1SF DALFNR INEL

GDGYKLAKIK

MOMLFTHFC I

SFVELLKDFQ

GGYNVTAAFV

I PGNGKFLYS

GVTVFTKTQV

GHTI SPLYPT

SGPAALRCSS

FIVTKTLPLE

TGHVAGSHAA

AFSQFDNYDI

TYKLLRKDDQI

ESPIISEEPF

F IN'QELTRNG

KSFVTGGGIS

E:IAEYTYLPI

MNFFESNG IH IGVETELEK I

ILDKTLQGLS

NQPVTVALDV

LKEVTPKTME

EE-V

EF085-1 (SEQ ID NO:321) a a a a

TAACCCATGA

CAGTCCACAC

CCGCGGTGTA

AAGGTT N'

TTTGCACAC

TATCAGCCAT

GGGCTGTT'C

GTACTCTATC

GTGGTACATA

CTTTATCAAG

CTTTTATOCG

AGTGCGAATA

CTTGGCGCAC

AGGCTGAG

GCGGCAGCCT

ATGCTTGAAA

ATGATGGACA

GGGAGAATCG

CCCGATTTC

GGACAAATTG

AATGCGGTTC

GAAAAATACC

AAGCTG ITCA

CGGTTTCGGGG

GGAAAACACC

AATATTTAA

AATCATTTTG

TCATATCAAA

GGTTACCGAT

CCTATACGAT

AATrTGTGTC

TGGTGGAACT

ACTACAATAA

CCACACCGAA

GAGATGATAA

GGCTGACAAT

CGTATATCTT

AAATGGCAAA

TGGCTCACGA

ATGAAATCGC

CTCATGAGTT

ATATCGGTGA

GCCAGGGCAA

TACCCATAGC

AAACCTTGGC

TCCTGTCCGP

AGAACACGCC

GTCTT'CCG]

TCCCATTCTI

CTTGCCATCC

TCAGATGGC(

TCCCGCATAT

AATCTGCGGG

TG-GAGGAATC

CATTGTCCTC

TTATTTCAGA

GATTCAGAAT

CCAATCCTTT

TGCCGATACA

TATI CGAT

TCGGGGAATT

TGCGCGGCAA

CCTGAAAGAA

CATGCATI'CC

AAGGGAACAT

AAAAACGCCC

*CTACAAAGAC

AACCATTTCC

*CGAACCGCTG

AGAGGCAA.AC

TCCGAAGCTC

CCAGGGAGGI

VrrGAACATC

TCGCATTGAI

TACAAAAAAC

T'1rTGTTCTC

GGGGATATGA

CGAACTGCCG

AMTATAATGG

TTACTCCTTG

GCGATGGAAG

AGCGATAGGC

GAGTITATA

TCAAATAGTG

G'IGCTCAAA

GTTATC.ATTf-

ATGACAACC

GTACCGCCGC

ATGTATATCA

GAGTTG-AGG

ATCGCGGCTG

CATTCTAAGT

GAAATACTGG

GA.CATAGGGC

AACCAGCGGI

ATCCAAAAGG

*GAG4GTACGGP

GGCGTTCAC;

CAGGCGCGAI

GCTGGATGCC

GCTGGATTTI

CTITGACGGT C

GAAAATATCA

AAAGAAAAGGC;

TCGrGTGTAAC

CACAGCAAAC

=TATATGCA

T'TGAAGATAA

TTAGGCCCGA

GCAAGGCAGG

CCATAATCGT

CGATCAAkAGC

CGCTGGAGCG

GGCTGAAACA

AAACACAGCG

TAAG-CGTTCT

ATCTGCGCGA

AGC 1TGTCAG

GCACGGT'GC

TCGTCACAGA

CGCTATCCAA

TATGGAGTGA

TTGATGATAC

SGCAGCAAAAA

ATGAGCCTCC

k CCGCCCACAT

,ATGGCAGCA

ECAAAACCAT

;ATTTrCATT

;GCAACACTG

AGTAAAATCC

AGAGGTGCA

AGAGGGAAGC

CTrTCTI'AT

TGTGGGATTG

TGTATTCAGC

CTTAGCGGAC

AAAGGATCAG

AACCATCGCA

ATATTTCTT

GTI'GGAGGGA

ATGCATCAAA

CCTGAACGAT

CGAGCTGCTA

TATIrCCAGCC

TGTCATAITG

GcCTGGGGCT

TGCACTTTCA

GTGGGCGMAG

AATATGCGCT

CAACACTATA

176 TABLE 1. Nucleotide and Amino Acid Seqeuences of E-faecalis Genes.

EF085-2 (SEQ ID NO:322)

HERKGIFIK

VFSYTI IVLL

L.FHYNNQSFE

YQGLTIRG IV GALAHDMI4SM

LENIGDYKDH

DFQTLAEANN

KYRLsvLNMCG

LLVGVTATLF

FYI EDKEGSV

MIAIMVVFSL

YIRLKETIAR

SKYLREC 1KM

QRFVTDIPAG

.VHIDDTALSK

AQQFVSYFRA

LYATPNADTS

LCAY IFARQM

LEDEIAREHE

MDRQGITISE

QIVLSDPKLI

LFIPFYRIDQ

MEAQQTVMSY

QPLVELIQNS

NSVRPDFLYV

VH-RODNISIV

7rTPIKALAIls

ANXMANLKEV

LEETQRYFFA

AASHELKTPI

ILELVSLNOG R IVP IAEPLD QKALSNVILN

AVQNTPQGGE

ARSSKK(WAKR

FGACHRTKNA

DRLDMQEVAG

AQSKAGVGLL

PPPLERKDEL

AAVSVLLEGM

IGRTVAELLP

VRIWSEPGAE

GcI{EPP ICAG KHLRWRFVLA GFTAHINTIN

I

EF08S-3 (SEQ ID NO:323) GC AATTTGTGTC 'FrATTTCAGA

GCGATGG

TATCAGCCAT TGGTGGA.ACT

GAFI'CAGALAT

AG CACAGCAA AC AGTAAAATCC

GGGCTGI'TC

GTACTCTATG

GTGGTACATA

CTTATCAAG

CT TTATGCG

AGTGCGAATA

CTTGGCGCAC'

AGGCTGGAGG

GCGGCAGCCT

ATGCTTGAAA

ATGATGGACA

GGGAGAATCG

CCCGATTI'C

GGACAAATTG

AATGCGGTITC

GAAAAATACC

AAGCTGTTCA

CGGTTTGGGG

GGAAA-ACACC

AATATTT

ACTACAATAA

CCACACCGAA

GAGATGATAA

GGCTGACAAT

CGTATATCT

AAATGGCAAA

TGGCTCACGA

ATGAAATCGC

CTCATGAGTI

ATATCGGTGA

GGCAGGGCAA

TACCCATAGC

AAACCTTrGCC

TCCTGTCCGA

AGA.ACACGCC

GTCTTTCCG!I

TCCCATTCTP

cTTGCCATCC

TCAGATGGCC

CCAATCCTTTr

TGCCGATACA

TATTTCGATT

TCGGGGAATT

TGCGCGGCAA

CCTGAALAGAA

CATGCAFI'CC

AAGGGAACAT

AAAAACGCCC

CTACAAAGAC

AACCATTICC

CGAACCGCTG

AGAGGCAAAC

TCCGAAGCTC

CCAGGGAGG

I

TTTGAACATC

TCGCATI'GA]

TACAAAAAA(

TTTTGTTCT(

GAGTTTTATA

TCAAATAGTC;

GTTGCTCAAA

GTTATGAT'IG

ATCACAACGC

GTACCGCCGC

ATGTATATCA

GAGTTGGAGG

ATCGCGGCTG

CATI'CTAAGT

GAAATACTGG

CACATAGGGC

AACCAGCGGT

ATCCAAAAGG

GAGGTACGGP

GGCGTTCACP

SCAGGCGCGAM

GCTCGATGCC

;GCTGGATr

TTGAAGAA

TTAGGCCCGA

GCAAGGCAGG

CGATA.ATGGT

CGATCAAAGC

CGCTGGAGCG

GGCTGAAAGA

AAACACAGCG

TAAGCGTTCT

ATCTGCGCGA

AGCTTGTC-AG

GCACGGT*TGC

TCGTCACAGA

CGCTATCCA-A

TATGGAGTGA

TTGATUCATAC

GCAGCAAA

ATGAGCCTCC

k CCGCCCACAI

AGAGGTGGCA

AGAGGGAAGC

CTITCTTTAT

TGTGGGATTG

TGTATTCAGC

CTTAGCGGAC

AAAGrGATAG

AACCATCGCA

ATATTTCTTT

GTTGGAGGGA

ATGCATCAAA

CCTGAACGAT

CGAGjCTGCTA

*TATTCCAGCC

*TGTCATATTG

GCCTGGGGCT

TGCACTTTCA

GTGGGCGAAG

pLATATGCGCT rCAACACTATA

S

S S EF085-4 (SEQ ID NO:324)

S

QFVSYFRA MEAQQWVKSY QPLVELIQNS

DRLDMQEVAG

LFHYNNQSFE FYIEDKEGSV LYATPNADTS

NSVRPDFLYV

YQGL.TIRGIV MIAIMVVFSL LCAYIFARQM

TTPIKALADS

GALARDMHSM YIRLKETIAR LEDEIAREHE

LEETQRYFFA

LENIGDYKDH SKYLRECIKM MDRQGKTISE

ILELVSLNDG

DFQTLAEANN QRFVTDIPAG QIVLSDPKLI

QKALSNVILN

KYRLSVLNMG VHIDDTALSK LFIPFYRIDQ

ARSSKKWAKR

KHiLRWRFVLA GFTAHINTIN

I

VHRIDDNIS IV

ANKMANLKEV

AAsHELKTP I RIVP IAEPLD

AVQNTQGGE

FGACHRTKNA

AQSKArGVGLL

PPPLERKDEL

AAVSVLLEGM

IGRTVAELLP

VRIWSEPGAE

GCHEPPICAG

EF086-1 (SEQ ID NO:325) TAAcTGGTGG

CATGATGACC

cTGTATGCAA

ACAGATATTT

GATTGGCAAA 'TCGTTCCGC

GCAGCGCTAA

TATI'GAATAC AGATGCAGAA

AAA'ITAAATA

AAGATCCAAA CATACA.ATGG

CCAATTTATC

CAATCACCGT TTTAGGTACT GGACTTF'rGT CAGATAcATT GATI1'TA 1TA AATTTACTGC TCcGcTGATG GTGCAACAGG

AGCTAACTTA

TAGAAGATAA TCAACGCCTA 1 177 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

GTACAAGTAC

TATCCTGATG

GAGTTGCTAA

AATGACCCTA

GTAAATGGAA

AkATCCTTTTA

GCAAAATTTG

CAACAAAGTG

GACTTGAAAA

AATGTATATG

AGTATGTATT

TGGCATACAG

GGATACTGGG

ITGGCAAATG

AATGACAGG

G 1TGCTAAAA

ACTGGTACGA

GAAGTGAAGC

ANTCCATTGA

ATAGAAGAAC

TATACAAATA

TACGAATArT

AAAGGCTATA

GTCATGATGA

ATGTCGGTTA

TTACAAAATA

GCGGACCCAG

GGCAGCTCGC

AAATTAATTC

GTCTACAGCG

GCAGAAGTAG

CCAACTAAAG

CAAGAAAAAG

CAA.ACTGTCG

CTACGTI'CGG

AAAAATGGGG

AAACAAGTTA

ACAGGAGAAA

TGTC T=~AG

AAGAAGCTGT

CTTCCMGAT

AAGGGTTTGG

ACA'ITAGTAA

AAATGCCATC

CTACAGAGTT

CACCAGAAAA

GGTCATACTA

ATGTAGTGAA

GTTCGATGGA

CACAACGTT

CAGACCOCAT

CAACGATCGA

GTGCTTATAC

TTGCCTCTAT

AATCTTGGTT

CAGATGCTTC

TTAACCAAGG

ATAACATTGG

GCTCTGGTCG

CATTTGCTAA

TAACAGTrGGT

CTGTTCTAGA

ATACATGGAA

=TCAGAAAA

ATGCATCCGT

AAATTn-CTGT

GTTCAATICAT

AGGAACAAAA

AAGCATTGAA

ACCAAGCAGA

AAGTAGATAA

ACTACACCGC

CAGATCAAAC

CAGTGAACG

GGAATAATGG

AGCCATCAAG

AGAAATCGAT

TTI=CGTAA

TCCGTCCGTT 1'AAAAAGTG TCAACATCGGT TATTTTCCGT ACGAATTCAG ACTATMIAC TTAI'TAAT GTTGTGGATA GATGGTTTCT GGTAGAACTA TGAATCGGGT AAAGAAACAA TTTAAGAAAT GACAT ITATA TCATTTCTTT AAAAAACCA-A TAGTGCGTCA CCTGCCCAAG TCGAGTCCTA CAGAAAAATA

CGGAAACTAT-GAATT=~GA

GCTTTATITA ThCPLATICAAG TCCATATCGA TTACCAGGAA AGGGAAACGC AGTCCCCAGT AGGAATGTTT TTAGATAAAA CTTATTAGAT GGTCAAATCA GATTGAAACA ATCCTCGATA TTCAGACAAA GATAAMTCTT CTATGTI'TT CCTAATTCNA CTACGGAGAT ATTAACGAAT AATTAGTAAA AATTATGGCA TGGGAAAACG AATGAACAAA AAATACAGCA AACTTACAAG TAATGACCAA GAAA CAG AATI'GATAAC GGTGTTTATC ITCTATTGAA TTTGATAAGG TGACCAAAAT ATATCACT' TGTTAAA.ACA ACTCCTMAAG AGAACACCAA GAAAAAGACT ACAAGCACAA ACTGTGGCAG AACAGAGT1A CGTTCGGCAG AACCAACTTG TrGAA.AATCA AAGCAGTTGG AAAGTCTACA AACAGCAACG CAAGCAGAAG ATTAACATTG AAAAATAGTG ACACTI'AAAT ACTAGTACAG CCAAGGTGGT TTCAGAAAAG CGCGCTTGTG ATIATTGGTC AAGTAAATCG AAGAAGTAA rTTrccTCTGG

ACAACGGCAG

AAGGTTCCGA

AAGGTTACTT

TTTCCAGAGC

TAGCTAA=r

CATCTATCCA

GAGATT=A

Cr-ACACCAAT

ACGAATATGC

ATACGGAAA.A

ACTI'TCTCA

CGACAGTTGA

CATGGGTAGG

GTAATCAAGG

TTAATTTGGG

ATCGGATGAT

GG;ATTAGTTT

TGAATACGCT

ACTTTGTTAA

AGACTGTTGA

TCGCAGCTCT

CCATTGAAGC

-ACTGTATGC

GCITAACTCT

GCATI'CTGA

TAAATAGTGC

TAACGAAAGA

ACACCGCAAG

ATCAA-ACAAC

TGAAGCAATT

TCAAAGAAAA

GTGAAGCATT

TAGACCAAGC

GGGAAAATAA

GAGMITATCA

CTACCCAATT

TCTAGTTAT

rGATGGCT-TA

TTACGGGAAT

CTGGGAGATG

ACAATTGATG

CCCAGAAACG

AACCTTAATT

AACGTGGCTT

AGCGTTAATT

GCAATCTTTA

GGTGGGGATC

TAAAAAAGC

GTTTGATGAA

CAC-AAGAGAA

TGGCTCAAAT

AATGAACTTA

AAGTGGCATT

TCATCCACAG

AAGCGCAGCG

TGATGTTCAA

TGATAAAACC

AAATGGTACT

TCTAAAAAC

AGGTAATTAT

CTATGATCCA

TGCGAATCCT

AGTAG.TCGCA

GGGGTTAAAT

AGCGTT AGAA

CAGCTGGAAA

AGCAACGCA.A

GGTAAAAGTG

CGAGAAACAC

GAAGCAAGCG

AGAAGCAAAA

AAAGGAGCAA

AACTGGTACG

T'ITACCGAGC

CGCCAGTGGG

S.

S.

S

555 S S

S

a.

S

S

S S a.

EF086-2 (SEQ ID NO:326)

IVGLANWFRA

DIS IT'JLTG

LLKGFGRIQT

PFTI'EFESGK

LKNVVNSASP

HTADGMLYLY

GQVAS IGMFL

VKLNQGSDKD

TNTFkAKISKN

MMNTWNNDQE

DPEISVDQNI

YSEALKQAQT

ALTDTLI LU-

LLLEDNQRLV

ILQGSDWEMN

ETIANLTLIA

AQATPMQSLN

NQDFAQFDEG

DKSNEGMNLV

NSWISLSAAX

YGKTVENGTY

IAGLYAYDPM

ITLNSAGLNG

VADQTTATQA

ODLLNTDAE(

QVQE-AVPSVL

DPNISNLFNV

KFAPENLRND

VYGSMDRVLQ

YWATIDPYRL

AKI(SWFLLDG

PLNNICYVFP

EYLTVVGKTN

SVISEKIDNG

SSRSI IVKTT

EVDQAETE-LR

LNKFTAPLML

KSVSSGDGLY

VDKGYLQLMV

IYTS IQTWLQ KNNEYAVG IS

PGITVDTREL

QI INLGSGIT

NSMNTLDVQI

EEIAALSKNK

VYRLTLANPL

PEVTKEA-LEK

SAVKQLVKVP

YAI(DPNIQWP

PDCGSLSIQHGY

NGKHPSMVSG

QSGSYYHFFK

MYSQRVGNYE

ANGAYTGKRS

GTDAS IETI

EERSGRYGDI

GYT'JLENTAN.

QNNASVSIEF

L IQEQKE{QE TK'tIMKTNLL I YRATG-ANLT

FPYNGSYGNE

RS ISRAPETN KPRDFEAkLID

FGNTENKKGW

PQSWVGGSNN

LDNRMI HPQE

NEYFVNDKTY

LQAIE-AGNY-V

DKGILEVVAA

KDYTASSWKV

KI IKENEKHQ TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

EKDYTASSWX, VYSE-ALKQAQ TVADQTTATQ AEVDQAEAKL RSAVKRLTLK

NSGENKKEQK

NGGNNGHLNT STGVDQTGTK QVKPSSQGGF RKASQFLPST G;EKKSIALVI

IGLLVIASGC

LLVFRKSKSK

K

EF086-3 (SEQ 10 NO:32 7 ACCAGAAALA TTAAGAA.AT GACATTTATA CATCTATCCA

AACGTGGCITT

cAACAAAGTG GGTCATACTA TCAT-rTT'r AAAAAACCAA

GAGA'TTGA

GACTTGAAAA ATCTAGTGAA TAGTGCGTCA CCTG-CCCAAG

CGACACCAAT

AATGTATATG GTTCGATGGA TCGAGTCCTA CAGAAAAATA

ACGAATATGC

AGTATGTATT CACAACGTGT CGGAAACTAT- GAA'11 GGGA ATACGGAAAA TGGCATACAG CAGACGGCAT GCTTTATTTA TACAATCAAG

ACTITGCTCA

GGATACTGGG CAACGATCGA TCCATATCGA TTACCAGGAA

CGACAGTTGA

TTGGCAAATG GTGCTTATAC AGGGAAACGC AGTCCCCAGT CATGrGGTAcr

AAT

GCAATCTTTA

GGTGGGGATc

TAAAAAAGGC

GTTTGATGAA

CACAAGAGAA

TGGCTcAA-AT EF086-4 (SEQ ID NO:32 8 PENLRND IYTSIQTWLQ QSGSYYHFFK

KPRDFEALID

LKNVVNSASP AQATPMQSLN VYGSMDRVLQ

KNNEYAVGIS

HTADGMLYLY NQDFAQFDEG YWATIDPYRL

PGTIVDTREL

MYSQRVGNYE FGNTEN1CKGW AINGAYTGKRS

PQSWVGGSNN

EF087-1 (SEQ ID NO:32 9 4

TAACTGGTGG

CATGATGACC

CTGTATCCAA

ACAGATATTT

GTACAAGTAC

TATCCTGATG

GAGTTGCTAA

AATGACCCTA

GTAAATGGAA

AATCCTTTTA

GcAAAATTXG

CAACAAAGTC

GACTTGAAAP

AATGTATATC

AGTATGTA19

TGGCATACAC

GGATACTGGC

TTGGCAAATC

AALTGGACAG(

GTTGCTA;Lk

ACTGGTACG.

GAAGTGAAG

ANTCCATTG

ATAGAAGAA

TATACAAAT

TACGAATA'I

AAAGGCTA7

GTCATGATC

ATGTCGGT]

TTACAWAN

GATTGCCAAA TJ TATTGAATAC

AC

AAGATCCAAA

C~

CAATCACCGTT

AAGAAGCTGT Ti GTTCCTTGAT

T

AAGGGTTTGG

A

ACATTAGT-

T

AAATGccATC

G

cTACAGAGTT

TI

CACCAGAAAA GGTCATACTA

I~

ATGTAGTGAA GTTCGATGGA

CACAACGTGTC

CAGACGGCAT

CAACGATCGA

GTGCTTATAC

STTGCCTCTAT

N. AALTCTTGGT1' A, CAGATGCTTC c: TrAAcCAAGG A ATAAcA'ITGG C GCTCTGGTCG 'A CATTI'GCTAA yr TAA.CAGTGGT ,A CTGTICTAGA ;A ATACATGGAA .A TITCAGAAAA C'A ATGCATCCGT EGGTTCCGC GC 3ATGCAGA6A bLTACAATGG

CC

rTAGGTACT

GC

:CCGTCCGT

CAACATGGT

T

cGAATTCAG A TTATTTAAT

G

,ATGGTTTCT

G

GAATCGGGT

TTALAGAAAT

G

~CATTTCTT 7AGTGCGTCA

C

M'GAGTCCTA

C

:GGAAACTATC

.CTTTATI'TA

rCCATATCGA

AGGGAAACGC

AGGAATGT

CTTATTAGAT

GAT1'GAAACA

TCAGACAAA

CTATGTTTTT

CTACGGAGAT

AATTAGTAAA

TGGGAAAACG

AAATACAGCA

TAATGACCAA

AATTGATAAC

TTCTATTGAA

AGCGrCTAA CAGATACATT

G)

A'ITAAATA AATTTACTGC

TC

:AATTTATC GTGCA.ACAGG

AC

;ACTTICT TAGAAGATAA

T

7AAAAAGTG TTrCCTCTGG Ti =TPTCCGT ACAACGGCAG

T

:TATTI'TAC AAGGTTCCGA

C

rTGTGGATA AAGGTT.ACTT

A

GTAGAAGTA TTTCCAGAGC

G

MLGAAAcAA

TAGCTAAITT

ACATTTATA

CATCTATCCA

AAAAACCAA GAGA'rTGA

P~

CTGCCCAAG CGACACCAAT

C

AGAAAAATA ACGAATATGC

C

AATTTGGCGA

ATACGGAAAA

'ACAATCAAG

ACTTTGCTCA

.'TACCAGGAA

CCACAGTTGA

GTCCCCAGT

CATGGGTAGG

n1AGATAAAA

GTAATGAAGG

-GTCAAATCA

TTAATTGGG

MLTCCTCGATA

ATCGGATGAT

GATAATTCTTI'

GGATTAGTTT

:CTAArTTCNA

TGAATACGCT

AT'rAACGAAT

ACTTTGTTAA

AATTATGGCA

AGACTGTTGA

AATGAAGAAA

T-CGCAGCTCT

AACTTACAAG;

CCATTGAAGC

G;AAATTGCAG GACTGTATGc G;GTGT'1rATC GCTrAACTCT TTT1GATAAGG GCATrCTTGA TTFTAT'rA

.CGCTGATG

;VcTAACTTA

.AACGCCTA

.,ATGGC

ITA

TACGGGAAT

TGGGAGATG

CAATTGATG

,CcAGAAACG

.ACCTTAAT

LACGTGGcTT

~GCGTTAAT

.CAATCTTTA

,GTGGGGATC

I.AAAAAAGGC

;TTTGATGAA

CACAAGAGAA

TGGCTCAAAT

AATGAAcTI'A

AAGTGGCATT

TCATCCACAG

AAGCGCAGCG

TGATGTCAA

TGATAAAACC

AAATGGTACT

TCTAAAAAC

AGGTAATTAT

GTATGATCCA

TGCGAATCCT

AGTAGTCGCA

TABLE 1. Nucleotide and Amino Acid Seqeueflces of E. facalis Genes.

GCGGACCcAG

GGCAGCTCC

AAATTAATTC

GTCTACAGCG

GCAGAAGTAG

CCAACTAAAG

cA.AGAAAAAG

CAAACTGTGG

CTACGTTCGG

AAAAATGGGG

AAAcAAGTTA

ACAGGAGAALA

TGTCTTTTAG

AAATTvTCTGT

GTTCAATCAT

AGGAACAAAA

AAGCATTGAA

ACCAAGCAGA

AAGTAGATAA

ACTACACCGC

CAGATCAAALC

cAGTGAAGCG

GGAATAATGG

AGCCATCAAG

AGAAATCGAT

TTTTTCGTAA

TGACCAAAAT

ATTATCACTT

TGTTAAAACA

AGAACACCAA

ACAAGCACAA

AAcAGAGTTA AACCAACT'rG

AAGCAGTTGG

AAAGCAACG

ATTAACATTG

ACACTTAAAT

CCAAGGTGGT

CGCGCTTGTG

AAGTAAATCG

CAAAAAGACT

ACTGTGGCAG

CGTTCGGCAG

TI2GAAAATCA

AAAGTCTACA

CAAGCAGAAG

AAAAATAGTG

ACTAGTACAG

TTCAGAAAAG

ATTAT'rGGTC

ACAAGTAA

TAAATAGTGC GGGGTTA.AAT TAACG-AAAGA

AGCGTTAGAA

ACACCGCAAG

CAGCTGGAAA

ATCAAkACAAC

AGCAACGCAA

TGAAGCAATT

GGTAAAAGTG

TCAA-AGAAAA

CGAGAAACAC

GTGAAcCATT

GAAGCAAGCG

TAGACCAAGC

AGAAGCAAAA

GCGGAAAATAA

AALAGGAGCAA

GAGTTGATCA

A.ACTGGTACG

CTAGCCAATT

TTTACCGAGC

TTCTAGTTAT

CGCCAGTGGG

EF0872-(SEQ ID NO:330)

LVGLANWFRA

DIS ITVLGTG

LLKGFGRIQT

PFTTEFESGK

LKNWVNSASP

HTADGMLYLY

GQVAS IGMFL VKL NQGSDKD TNTFAKI SKN

MM~NTWNNDQE

DPEISVDQNI

YSEALKQAQT

EKDYTASSWK

NGGNNGHLNT

ALTDTLILLHI

LLLEDNQRLV

ILQGSDWEMN

ETIANLTLIA

AQATPMQSLN

NQDFAQFDEG

DKSNEGMNLV

NSWISLSAAX

yGKTVENGTY

IAGLYAYDPM

ITLNSAGLNG

VADQTTATQA

vysrEALKQAQ

STGVDQTGTK

DDLLNTDAEKI

QvQEAVPSVL

DPNISNLFNV

KFAPENLRND

VYGSMDRVLQ

YWATIDPYRL

AKKSWFLLDG

PLNNIGYVFP

EYLTVVGKTN

SVISEKIDNG

SSRSI IVKTT

EVDQAETELR

TVADQTTATQ

QVKPSSQGGF

.NI(F1APLMLJ

(SVSSGDGLY

IDKGYLQLV

IYTSIQTWLQ

KNNEYAVGIS

PGTTVDTREL

QI INLGSGIT NSMTLDVQ

I

EE-IAALSY.NK

VYRLTLANPL

pEVTKEALEK

SAVYKQLVKVP

AEVDQAEAKL

RK(ASQFLPST

YAKIDPNIQWP I PDGSLIQ{GY

I

NGKM-PSMVSG

QSGSYYHFFK

i4YSQRVGNY-E ANGAY-TGKRs GTTD ASIETI

EERSGRYGDI

GYTVLEI\TAN'

QNNASVS IEF

LIQEQKEHQE

TKEVIDKTNLL

RsAVKRLTLK GEKXS IALVI :yRATGANLT

~PYNGSYGNE

ISI SRAPETN KPRDFEAL ID

E'GNT-ENKKGW

PQSWVGGSNN

LDNFRMIHPQE

NEYFVNDKTY

IQAIEAGNYV

DKILEVVAA

KDYTASSWKV

KI1IKENEKHQ

NSGENKKEQK(

IGLLVIASGC

4

S

S 9 a

S

a a *9 LLVFRKSKSK K EF087-3 (SEQ ID NO:331) A ATCGGATGAT

TCATCCACV

GAAGTGAAGC

TTAACCAAGG

ANTCCATrGA

ATAACATTGG

ATAGAAGAAC-

GCTCTGGTCG

TATACAAATA CAIrCTAA TACGAATATI

TAACAGTGGT

AAAGGCTATA

CTGTTCTAGA

GTCATGATGA

ATACATGGAA

ATGTCGGT'TA TT1'CAGAAAA TTACAAAATA

ATGCATCC

TrTCAGACAAA GATAATTCTTI

CTATGTI'TT

CTACGGAGAT

AATI'AGTAAA

TGGGAAAACG

AAATACAGCA

TAATGACCA

AATTGATAAC

ATAACGAAT

AATTATGGCA

AATGAAGAA

AACTTACAAG

GAAATTGCAG

GGTGTTTATC

GGATTAGTTTI

TGAATACGCT

ACTTTGTTAA

AGACTsTTGA TC-CAG CT1CT CCA 1TGAAGC

GACTGTATGC

GCrAACTCT

AAGCGCAGCG

TGATGTTCAA

TGATAAAACC

AAATGGTACT

TT~CTAAAAAC

AGGTAAT1TAT

GTATGATCCA

TGCGAATCCT

EF087-4 (SEQ ID NO:3 3 2 NRM4IHPQE VKLNQGSDKD NSWISLSAAX pLNN'IGYVFP NSb4NTLDVQI EERSGRYGDI

NEYFVNDKTYI

TNT1FAKISKN YGKTVENGTY EYLT'JVGKTN EEIAALSKNX GyTVL.ENTAN

LQAIEAGNYV

MMN~TWNNDQE IAGLYAYDPM SVISEKIDNG VYRLTL.ANPL

QNNAS

EFO88-1 (SEQ ID NO:3 3 3 180 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faccalis Genes.

TAACTGGTGG

CATGATCACC

CTGTATGCAA

ACAGATMTT

GTACAACTAC

TATCCTGATG

GAGTTGCTAA

AATGACCCTA

GTAAATGGAA

AATCCTTTI'A

CCAAAATTTG

CAACAAAGTG

GACTTGAAAA

AATGTATATG

AGTATGTATT

TGGCATACAG

GGATACTGCG

TTGGCAAATG

AATGGACAGG

GTTCCTAAAA

ACTGGTACGA

GAAGTGAAGC

ANTCCATTGA

ATAGAAGAAC

TATACAAATA

TACGAATATT

AAAGGCTATA

GTCATGATGA

ATGTCGGrA

TTACAAAATA

GCGGACCCAG

GGCAGCTCGC

AAATTAATTC

GTCTACAGCG

GCAGAAGTAG

CCAACTAAAG

C.AAGAAAAAG

CAAACTGTGG

CTACG~rCGO

AAAAATCGG

AAACAAGITA

ACAGGAGAAA

TGTC=TAG

CATTGGCAA.A

TATTGAATAC

AAGATCCAAA

CAATCACCGT

AAGAAGCTGT

GtTCCTTGAT AAGGG ITTGC

ACATTAGTAA

AAATGCCATC

CTACAGAGT'

CACCAGAAAA

GGTCATACTA

ATGTAGTGAA

GTTCGATOGA

CACAACGTGT

CAGACGGCAT

CAACGATCGA

GTGCTTATAC

TTGCCTCTAT

AATCTTGGTT

CAGATGCTTC

TTAACCAAGG

ATAACATTG

GCTCTGGTCG

CATTTGCTAA

TAACAGTGGT

CTGTTCTAGA

ATACATGGAA

TTCAGAAAA

ATGCATCCGCI

AAATTrTCGI

GTTCAATCAI

AGGAACAAA-a

AAGCATTGAP

ACCAAGCAG;

AAGTAGATA-Z

ACTACACCGC

CAGATCAAAC

CAGTGAAGCC

GG.AATAATGC

AGCCATCAAC

AGAAATCGA'

TTTTCGT;J

TTGGTTCCGC

AGATGCAGAA

CATACAATGG

TTTAGGTACT

TCCGTCCCGTT

TCAACATGGT

ACGAATTCAG

11TATTTAAT

GATGGTTCT

TGAATCGGGT

ITTAAGAAAT

TCATTTCTTT

TAGTGCGTCA

TCGACTCCTA

CGGAA-ACTAT

G-CTTATTTA

TCCATATCGA

AGGGAAACGC

AGGAATGT'T

CTTATTAGAT

GATTGAAACA

TTCAGACAAA

CTATGTTTTT

CTACGGAGAT

AATTAGTAAA

TGGGAAAACG

AAATACAGCA

TAATGACCAA

AATTGATAAC

TTCTATI'GAA

TGACCAAAAT

TGTTAAAACA

ACAACACCAA

ACAAGCACAA

AACAGAGT'A

AACCAACTTC

AAGCAGTTGC

AACAGCAACC

ATrAACATTC

ACACTTAAAI

CCAAGGTGGI

r' CGCGCTIlGTC k AAGTAA-ATCC

GCAGCGCTAA

AAATTAAATA

CCAATTTATC

GCACTTTTGT

TTAAAAALGTG

TATTTTCCGT

ACTATTTTAC

GTTGTGGATA

GGTAGAAGTA

AAACAAACAA

GACATTTATA

AiAAACCAA

CCTGCCCAAG

CAGAAAAATA

GAATTTGGGA

TACAATCAAG

TTACCAGGAA

AGTCCCCAGT

TTAGATAAAA

GGTCAAATCA

ATCCTCGATA

GATAAI'TCT

CCTAATTCNA

ATTAACGAAT

AAT'IATGGCA

AATGAAGAAA

AACTrACAAG

GAAATTGCAG

GGTGTTTATC

T=IATAAGG

ATTATCACTT

ACTCCTGAAG

*GAAAAAGACT

*ACTGTGGCAG

CGI'GGCAO

TTGAAAATCA

1AAAGTCTACA

CAACCAGAAC

AAAAATAGTC

ACTAGTACAG

TCAGAAAAC

ATTATTGGTC

AAGAAGTAA

CAGATACATT

AATTTACTGC

GTGCAACAGG

TAGAAGATAA

=rCCTrCTGG

ACAACGGCAG

AAGGTTCCGA

AAGG7TACTT

TTTCCAGAGC

TAGCTAATTT

CATCTATCCA

GAGATTTTGA

CGACACCAAT

ACGAA'VATGC

ATACGGAAAA

ACTTTGCTCA

CGACAGTI'GA

CATGGGTAGG

GTAATGAAGG

TTAA7TTGGG

ATCGGATGAT

GGATTAGTTT

TGAATACGCT

ACTTT GTTA.A

AGACTGTTGA

TCGCAGCTCT

CCATTGAAC

GACTGTATGC

GCITAACTCT

GCATTCTI'GA

TAAATAGTGC

TAACGAAAGA

*ACACCCCAAG

ATCAAACA-AC

TGAAGCAATI

*TCAAAGAAAA

GTGAAGCATI

TAGACCAAGC

GCAAAATAP

GAGTTlGATCP CTAGCCAAT7

*TTCTAGTTAI

CATTTTATTA

TCCGCTGATG

ACCTAACTTA

TCAACCCCTA

TGATGGCTTA

'FrACGGGAAT

CTGGGAGATG

ACAATTGATG

GCCAGAAACG

AACCTTAATT

AACGTGGCT

AGCCTTAATT

GCAAT'CTIA

GGTGGGGATC

TAAAAAAGGC

GTTTGATGAA

CACAAGAGAA

TGGCTCAAAT

AATGAACTTA

AAGTGGCATT

TCATCCACAG

AAGCGCAGCG

TGATGTTCAA

TGATAA.AACC

AAATGGTACT

TTCTAAAAAC

AGGTAATTAT

GTATGATCCA

TGCGAATCCT

AGTAGTCGCA

GGGGTTAAAT

AGCGTTAGAA

CAGCTGGAAA

AGCAACGCAA

GGTAAAAGTG

CGAGAAACAC

GAAGCAAGCG

,AGAAGcAAAA

,AAAGGAGCAA

,AACTGGTACG

TITACCGAGC

CGCcAGTGGG EF088-2 (SEQ ID NO:334)

LVGLANWFRA

DISITVLGTG

LLKGFGRIQT

PFTTEFESGK

LKNVVNSASP

HTADGMLYLY

GQVASIGM2FL

VKLNQGSDKD

ALTDTL.ILLH

LLIJEDNQRLV

ILQGSDWEMN

ETIANLTLIA

AQATPMQSJN

NQDFAQFDEG

DKSNEMN~LV

NSWISLSikX

DDLLNTDAK

QVQEAVPSVL

DPNISNLFNV

KFAPENLRND

VYGSMDRVLQ

YWATIDPYRL

AKXSWFLLDG

PLNNIGYVFP

LV1FnAPLML

KSVSSGDGLY

VDKCYLQLMV

IYTSIQ IWLQ KNNEYAVG IS

PCTI'VDTR:EL

Q IINLGSGIT

NSMNTLDVQI

YAXKDPNIQWP

PDGSLIQHGY

NGKMPSMVSG

QSGSYYHFFK

MYSQRVGNYE

ANGAYTGKRS

GITDAS IETI

EERSGRYGDI

iyRATGANLT

FPYNGSYCOE

RSISRAPETN

KPRDFEALID

FGNTENKKGW

PQSWVCGSNN

LDNRMIHPQE

NE-YFVNDI<Ty 181 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

TNTFAKISKN

MMTWNNDQE

DPEI SVDQNI

YSEALKQAQT

EKDYTASSW(

NGGNNGHLNT

LLVFRKSKSK

YGKTVENGTY

IAGEJYAYDPM

ITLNSAGLNG

VADQTTATQA

VYSEALKQAQ

STGVDQTG"X,

EYLTVVWGKTN

SVI SEK lONG SSRS IIVKTT

EVDQAETELR

TVADQTTATQ

QXTKPSSQGGF

EEIAkALSKNK

VYRLTLANPL

PEVTKEALEI(

SAVKQLVKVP

AEVDQAEAKL

RKASQFLPST

GYTVLENTAN

QNNASVSIEF

LIQEQKEHQE

TXEVDKTNLL

RSAVKRLTLK

GEKKS IAIVI LQAI EAGNYV

DKGILEVVAA

KDYTASSWKV

KI IKENEKNQ

NSGENKKEQK

IGLLV IASGC EF088-3 (SEQ ID NO:335) A ACTCCTGAAG TAACGAAAGA AGCGTTAGAA AAATTAATI'C AGGAACAAAA AGAACACCAA dAAAAAGACT GTCTACAGCG AAGCATTGAA ACAAGCACAA ACTOTOCCAG GCAGAAGTAG ACCAACCAGA AACAGAGTTA CGTTCGGCAG CCAACTAAAG AAGTAGATAA AACCAACTTG TTGAAAATCA CAAGAAAAAG ACTACACCGC AAGCAGTTGG AAACTCTACA ACAC CGCAAG

ATCAAACAAC

TGAAGCA.ATT

TCAA.AGAAAA

GTGAAGCAF1'

CAGCTGGAA-A

AGCAACGCAA

GGTAAACTG

CGAGAAACAC

GAAGCAAGCG

CAAACTGTGG

CTACG'ITCGC

AAAAATGGGG

AAACAAGTTA

CAGATCAAAC

CAGTGAAGCG

GGAATAATGG

AGCCATCAAG

AACAGCAACG

ATTAACATTG

ACACTTAAAT

CCAAGGTGGT

CAAGCAGAAG

AAAAATAGTG

ACTACTACAG

TTCAGAAAAG

TAGAC-A-AGC AGAAGCAAAA GGGAAkAATAA

GAGTTGATCA

CTAGCCAAITT

AAAGGAGCAA

AACTGGTACG

TTTACCGAGC

ACAGGAGAAA AGAAA EF088-4 (SEQ ID NO:336) T PEVTKEALEK LIQEQKEHQE KDYTASSWKV YSEALKQAQT VADQTTATQA EVDQAETELR SAVKQLVKVP EKDYTASSW< VYSEALKQAQ IVADQTTATQ AEVDQAEAKL NGGNNGHLNT STGVDQTGTK QVYPSSQGGF RKASQFLPST

TKEVDKTNLL

RSAVKR:LTLK

GEKK

KI IKEN'EKHQ

NSGENCKEQK

9 9 .99.*

C

EF089-1 (SEQ ID NO:337)

TGACAGATAC

TATAGGTCAA

AATGACAGAC

TGTATGTTAT

ACACCAACAA

GCGCCTGGTG

ACCA'ITGAAA

CAAAACGGGA

CCGAAAGAAA

CCTAAAGATT

GAAACAACGA

GTTGTGGCTA

GGGGTTAAAC

CAAGCGGCCT

CTI'TACCAAT

ATTrCTTTAA

GGTGTAAAAG

CTGTACAAAT

AATGAAAAAG

ATCATTCTAG

GAACAACAAT

ACCTGCTAAC

AAATTTTTTG

ATAGGAGAAT

ETGGCTGGAT

ITCCCGAAAA

CCAAACAAAC

ATACGGTGA.A

TCAAACCTGA

TCATCTTGCC

CT'=TTATGG

CTTCTGCGGA

ETATTCTrCA

CAGGCCAAGT

ATTI'AAACCA

CCGATACTGA

AAGGGGAACG

ATGAAAAGGG

GGGAATrTAC

ACCTAACCAT

CGCTG.CTCTT

CTGAGCAATA

ACAGGAAACT

GCTTATCTT

GAATATGAAC

TGGCGTGGAG

TCAAGTG-GAT

CGTAGAAATT

CTCAGCGACA

CAAAACCT1'A

GAAGCATTCC

CGTGATGGCT

TCAATCAAAA

GCAAAATGAG

CAACGCGCGA

ATTACATITA

GGATATGCAA

ATTAACGCCA

CACCTATCAA

AAAAGAAT=

TAAAGGAACC

ATTGATITC

A

AAGAACGACA

CGGTCTTTG

AGATGGAAAG

GCGCACGCTT

AAATCAAAAA

CAGITACGCA

ACAAATTTAA

CG T=IAACT

CAAAAGACCT

GGCGGTATAA

GGGTI'AGCTA

ACAAAGGTTC

AACGTCATCA

ATCAACACTG

GTGGCGCCAA

GGAAAATATG

GTCAAAGGCG

ACTATTTCTG

AATTGGTGGT

TTCTTGTATC

CCATACACGC

GTCCTTATAA

TATATGCAAC

CTGAA=AA

CCTACT=TA

AT GATACAGA

ATGGCGTAGT

TAAAAGA'ITA

TACCTTTAAC

CACTCAAAGA

TTAATAATGA

AACCAGATT

ATGT=CT'Fr

TTTCAAAAGG

ACTCTAAC'rr

TCT'TGAAATC

CCAATGGTGA

GGGACGTGC

TGTATCT'ACT

GTAAAAAGAA

AAGATCGGGA

TACAACAAAG

GGTAATCGCT

TITTGCGGTC

CTTAA.AAATG-

TGAAGACATT

AGAATATGGC

TGTGGAAGCA

CATTACGATG

GAAAAAGAAA

ATACTCCTAT

AAAATTACTG

ACAAAACCCA

AGGCGAAACG

TAGTTACCCA

AACGGCCTAT

AGAACGGTAC

TAAAGAATTA

GATTGCATTA

AAAAGAGGAA

EF089-2 (SEQ ID NIO:338) 182 TABLE 1. Nucleotide and Amino Acid Scqeucnces of E. faecalis Genes.

MNR WKWVATVIAC MLFGWIGVEA HASEFNFAVT IEN'IVNSATT NLNGVVEYGQ KDSFDGVMAG GITLKEKKKE VKPGQVNARN VINVSLQNPQ SLXGERLTPG KYVLKSTAYG EKDVTIKGTN WWLYLL IAL I

PTIPENQVDK

NG IKPCKTLR

TTTSADQSKG

AAYLNQLHLI

VKDEKGTYQV

ILALLLLIFF

SKTYFDLKMA

FNLKDYVEAP

LAINNEYSYV

NTVSKGGETL

KGANGEERYL

LYRKKKKEEE

PGA(Q'VEI Q

XEIILPKHSQ

VAI ILQQNET

YQSDTEDMQV

YXWEFTK-FT

QQSEQ

LRNDTDED IT

KTLPLTITMP

KVQPDLKLLG

AFNSNFSYP I

ISCDVAKELN

EF089-3 (SEQ ID NO:339) T CTGAA TTAA TTTTGCGGTC

ACACCAACAA

GCGCCTGGTG

ACCATTAAA

CAAAACGGGA

CCGAAAGAAA

CCTAAAGATT

GAAACAACGA

GTTGTGGCTA

GGGG'ETAAAC

CAAGCGGCCT

CTTTACCAAT

ATTTCTTTAA

TCCCGAAAA

CCAAACAAAC

ATACGGTG;A

TCAAACCTGA

TCATCTTGCC

CTTTTGATGG

CTTCTGCGGA

TTATTCTTCA

CAGGCCAAGT

ATTIAAACCA

CCGATACTGA

A.AGGGGAACG

TCAAGTGGAT

CGTAGAAATT

CTCAGCGACA

CAAAACCTTA

GAAGCATTCC

CGTGATGGCT

TCAATCAAAA

GCAAAATCAG

CAACGCGCGA

ATTACATTTA

GGATATGCAA

AT

AAATCAAAAA

CAGTTACGCA

ACAAATTTAA

CGTTITAACT

CAAAAGACCT

GGCGGTATAA

GGGTTAGCTA

ACAAAGGTTC

AACGTCATCA

ATCAACACTG

GTGGCOCCAA

CCTACTTTGA

ATGATACAGA

ATGGCGTAGT

TAAAAGATTA

TACCTTTXAC

CACTCAAAGA

TTAATAATGA

AACCACATT

ATGT=C=T

TTTCAAAAGG

ACTCTAACTT

CTTAAAA.ATG

TGAAGACATT

AGAATATGGC

TGTGGAAGCA

CATTACGATG

GAAAAAGAAA

ATACTCCTAT

AAAATTACTG

ACAAAACCCA

AGGCGAAACG

TAGTTACCCA

EF089-4 (SEQ ID NO:340) S Sc. SEFNFAVT PTIPENQVDK SKTYFDLKMA PCAXQ'IVEIQ LANDTIDEDIT IENTVWSATT NLNGVVEYGQ NGIKPDKTLR FNLKDYVEAP KEIILPKHSQ KDSFDGVMAG GITLKEKKKE TTTSADQSKG L AINNEYSYV VAIILQQNET VKPGQVMARN VINVSLQNPQ AAYLNQLHLI NTVSKGGETL YQSDTEDMQV

SL.KGER

KTLPLTITMP

KVQPDLKLLG

APNSNFSYP I EF090-1 (SEQ ID NO:341)

SS

a

*S.S

S

aS S S a.

TAGTCTCTAA

GAAATGCAAA

ATTATTACTT

AGTCACTITT

GCTGCAGAAG

ACAAAAGAAG

GACCA TTAC

GAAGCCAAAT

CGTCGAGGCA

CAGCGCTTAA

AATGCCAAAG

ACATTGACAA

AAAGTAGCCT

AAAGAAAAAC

CCAGTTAT

GGCTGGTCGA

GAACAAACGA

CCCAATAATT

AAAAAAATAG

GAAATAXACC

TGCGTAATAC

CFTGGTTGG

TNCCAACCAC

AAAAA'ITAC

AAAAAAT TCA

ACAGTAGCAA

TAGCAACTTT

ATGGCAC'IFT

ACCAGCAGAT

ATTTTTATAA

CCCTCAATAA

TTGATAAAAC

TAACTACTTG

TTACAGATGT

TTGATGGGGC

ATGCAATCAC

ACATTGAAAT

TCACCACAGA

TAAAATTATT

ACGTCGTCAA

ACTAGCTCTG

AAAAGTAAkAT

GCAAGTTAAT

ACTTCCTAAA

GGTGAAGCTA

GCGTTTTCCA

TGAAATTGT

TATTGCGGAT

AGCCCCTGAA

CAAGTTAAAT

ACAAATTCAA

GGTGACACAA

TCACGGCACG

CAAAACGCAA

TGCTCCGTTG

TGATTIAAAA

TGTCATTACT

GATATAAAGG

AAATCAGGAA

GTTGCTG-GGG

GGAGTTTCTG

CAAACCGAAG

AAATACCAAT

CCGTTAA-ACG

GAGGGGAMAC

CCCGAAGAAC

GTGAAGCGG

ATTACA-AAAG

AAAACAATA

AACGTGCTGA

TTAGAAACAA

ACACGTCG ET

GAACTACTAG

GTTGGTGATA

GATCAAAAAA

GGCAGATATA

ATCAACAAAT

PIAAATAATAA

GCAGTTATGT

TAGGCTGGT

AAGITMGGT

TGGATCAAAA

AGGCATTCAA

CAACCAAAAA

AAGGCACAGT

GAAAAGGCAA

AGGATCAAAC

CAGTTGATAT

ATCATGATGG

CATATGGTTC

TTAAAAACAA

TAAACGCGCT

CCAAAGAAAA

TGTA TGTTT

ACAAAGGAAC

GAAAAAAGAA

AAAGAAAGTA

TTATTI TCAA

AAATGTAAAT

TCAAACGGGG

AT ITTTAAAA

AAAAGAACTA

TGcGAGTATC

AGTGGACACA

CTATCAATAT

GTrAA.AGGCA

TAATGGTGAA

CACAATCAAC

TCCTAATCAA

CGGAAG'ITAT

GAATAGCCAA

TAGTAAAATT

CATTGATGGC

CGCAACAGTA

183 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

CCAGGATTCC

GATGGITCTC

GTGTCACAC

GAAGCCTTTA

TCAAAAATCT

GATGATGCAC

ATACAAI-lP

GATACAGTGT

AAATCGGGAT

AAACCCATGC

TTGATGTATC

CAGGTGAATT

ATATCGGACA

GCCAGTAAAA

TCATGACTCC

TGGTACTAAT

CTATGACGGA

TGATAAACCT

ACCCAI'GTGA

TATTGGATGC

GACCATAAAT

GGCTGTATCA

ATGCCGGTXA

GTAGAI'TACG

ATTTAG7LAGG

CGTTATTAAG

TGGATAAGTA

ATACGCCAGG

TTATTTATGG

GCGAAGAAGT

TCAAATGCTT

TCAAGGGGGC

TGGCGATAAA

AACAGA.AGTT

ACATATCTAT

ATAA

EF090-2 (SEQ ID NO:342) MRNTRRQK SGKNNXKKVI ITSLVGLALV AGGSYVYFQS

HFXPTTKVNG

HLHSSKVKLP

RLNQQ IIADV

VAFDKTQIQN

WS IDGAKTQE

KIVTTDVITG

VSVGWLNVNA

LNEAFKKELE

EACKGNYQYN

VLNODGTINK

LLVNALNSQE-

RYNKGTATVP

AEEKLAQVNQ

A.KLATLSFPE

AKDFYKAPEI

EKLTTWVTQL

QTNAITAPLV

GFH-TILYRTT

AFXTOAGSNG

TEEVVVQTGT

GKPSKNAS IR

TKEDQTLXAT

ETTYGSANQP

GDTKENSKIA

DVNLEGQMLD

CI NTPGTEVS KEEK IQLPKK

RGNGTFEIVP

LTTLN~kLNK

VLFTDVHGTT

NNYIEIDLKD

GSRYSVPVKY

KIFDVSYDGM

YQLDQKFLKD

EEQGTVVDTQ

TITVDINGEK

RRFKNNGSYG

QKMYCFIDGK

WMPLLSQGGV

PVIIYGHIYD

VTQ IGIHDSD HKLDKYGDKE DAPGEFDKPV DYGEEV EF090-3 (SEQ ID NO:343) CAC AAAAGTAAAT GGAGTTTCTG TACGCTGGTT AAATGTAAAT 9 9 9 9 *9*e

GCTGCAGAAG

ACAAAAGAAG

GACCATTTAC

GAAGCCAAAT

CGTCGAGGCA

CAGCGCTTAA

AATGCCAAAG

ACATTGACAA

AAAGTAGCCT

AAAGAAAA.AC

CCAGTTTTAT

GGCTGGTCGA

GAACAAACCA

GCCAATAATT

AAAAAAATAG

CCAGGATTCC

GATGGTTCTC

GTTGTCACAC

GAAGCCTTTA

TCAAAAATCT

GATGATGCAC

AAAAATTAGC

AAAAAATTCA

ACAGTAGCAA

TAGCAACTTT

ATGGCACTTT

ACCAGCAGAT

A'TFI'TATAA

CGCTCAATAA

TTGATAAAAC

TAACTACTTG

TTACAGATGT

TTGATGGGGC

ATGCAATCAC

ACATTGAAAT

TCACCACAGA

ATACAATTTT

GATACAGTGT

AAATCGGGAT

AAACCGATGC

TTGATGTATC

CAGGTGAATT

GCAAGTTAAT

ACTTCCTAAA

GGTGAAGCTA

GAG'ITTTCCA

TGAAATTGTT

TATI'GCGGAT

ACCCCCTGAA

CAAGTTAAAT

ACAAATTCAA

GGTGACACAA

TCACGGCACG

CAAAACGCAA

TGCTCCGTTG

TGATTTAAAA

TGTCATTACT

ATATCGGACA

GCCAGTAAAA

TCATGACTCC

TGGTAGTAAT

CTATGACGGA

TGATAAACCT

CA.AACCGAAG

AAATACCAAT

CCGTTAA.ACG

GAGGGGAAAC

CCCGAAGAAC

GTTGAAGCGG

ATTACAAAAG

AAAACAATTA

AACGTGCTGA

TTAGAAACAA

ACACGTCGTT

GAACTACTAG

GTTGGTGATA

GATCAAAAAA

GGCAGATATA

ACCGATGTGA

TATTGGATGC

GACCATAAAT

GGCTGTATCA

ATGCCGGTAA

GTAGATTACG

AAGTTGTGGT TCA TGGATCAAAA ATT AGGCATICAA AAA CAAGCAAAAA MC AAGGCACAGT AGT GAAAAGGCAA CTA AGGATCAAAC GTT CAGTTGATAT TAA ATGATGATGG CAC CATATGGTTC TGC TTAAAAACAA CGG TAAACGCGCT GAA CCAAACAAAA TAG TGTATTGTTT CM ACAA.AGGAAC CGC ATTTAGAAGG TC~ CGTI'A1 9 AAG TC TGGATAAGTA TGC ATACGCCAGG AAC TTA=FATGG ACI GCGAAGAAGT AT

AACGGGG

TTTAAAA

GAACTA

GAGTATC

OGACACA

TCAATAT

AAAGGCA

.GTGAA

AATCAAC

TAATCAA

AAGTTAT

*TAGCCAA

~TAAAATT

,TGATCGC

:AACAGTA

LAATGCTT

LAGGGGGC

;CGATAAA

AGAAGTI'

CTATCTAT

*993 9 *9 S

S

EF090-4 (SEQ ID NO:344) TKVbIG VSVGWLNVNA AEEKLAQVNQ TEEVVVQTGT KEEKIQLPKI< YQLDQKFLKD HLHSSKVKLP LNEAFKKELE AKLATLSFPE GKPSKNASIR RGNGTFEIVP EEQGTVVDTQ RLNQQIIADV EAGKGNYQYN AKDFYKAPEI TKEDQTLKAT LTTLNNXLNX TITVDINGEK VAFDKTQIQN VLNDDGTINK ELTTWVTQL ETTYGSANQP VLFTDVHGTr RRFKNNGSYG WSIDGAKTQE LLVNALTNSQE QTNAITAPLV GDTKENSKIA NNYIEIDLKD QKMYCFIDGK KIVTTDVITG RYNKGTATVP GFHTILYRTT DVNLEGQM~LD GSRYSVPVKY WMPLLSQGGV VTQIGIHDSD HKLDKYGDKE AFKTDAGSNG CINTPGTEVS KIFDVSYDGM PVIIYGHIYD 1 184 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

DAPGEFDKPV DYGEEV EF091-1 (SEQ ID NO:345)

TAATTGGNGG

GGAACAGCAG

rCATGATTTAT

GCTGTTCAAA

GATCAAGCCT

GCACAAGGC

GATCAA'IT1A

GCCGAAGATG

GAAACTG'TT

GCTGCAGAAG

GGGTAA

AGATTTTTAT

CAGCCGTTGC

CAAATCAAAC

AAGGAACAGA

CTGATTGGC

TTTCTGGCGA

AAAAAGCAGC

CAGCAGAAGA

CTGCTCCTT

CAAAAGAAGA

GGCTAAAAAA

CGCATTAITA

AGATGATTTA

ATTAACAGAA

AGGT'TCTGTC

CATGCTTGAT

TrGACGATGCT

TATCTATATT

CGATGAAGCA

TGTTAAAT

GGCGGATTTT

CTI'GCACCAA

AAAAACAAAG

ATCGCAAAAC

AAAGAAAAAA

AACTTTkAAA

CAAGATCACG

&AECGT-TAAAG

ANAGAAACCA

GCAGCGAAAG

TCTTAGGNGC

AATCAGGTAA

CCCAAGATTA

AAAAAGCCGG

CAAAAGATTC

AACA-AACAGG

CAGAAGATTT

ATTCTGCGGC

CCA-AAGATGT

ACGTAIAAAAA

AGTAATTGGT

AGAATTACGT

CACAGATTAT

CGTTTTATCA

ATTGGATAAA

TGATTTATCT

AGGTGAA-AT

AGCGGCCAAA

TCCTGAAAAA

AGAATTTAAA

EF091-2 (SEQ ID NO:346) MAKKC GFFLGAVIGC TAAAVAALLL APKSGKELRD OLSNQTDDU( NKAQDYTDYA VQKGTELTEI AKQKAGVLSD QASDLAGSVK EKTKOSLDKA QGVSGDMLDN FKKQTGDLSD QFKK.AADDAQ DHAEDLGE IA EDAABD IY ID VK.DSAAAAKE TVSAGVDEA.X ETTKDVPEKA AE-AKE-DVKDA AKDVKKEFKG EF091-3 (SEQ ID NO:347) AT CAAATCAAAC AGATGATTTA AAAAACAAAG CGCAAGATTA CACAGATTAT 90 0 0 0@ 00 0 9 0* 00 0 0 00 00 9 000 0 00 0 0 .00 0 GCTG TTCAAA

GATCAAGCCT

GCACAAGGTG

GATCAAT'rA

GCCGAAGATG

GAAACTGTITT

GCTGCAGAAG

AAGGAACAGA

CTGAI=TGC

TTTCTGGCGA

AAAAAGCAGC

CAGCAGAAGA

CTG-CTGGTGT

CAAAAGAAGA

ATTAACAGAA

AGGTTCTGTC

CATGCTTGAT

TGACGATGCT

TATCTATATT

CCATGAAGCA

TGTI'AAAGAT

ATCGCAAAAC

AAAGAAAAAA

AACTTTAAA

CAAGATCACG

GACGTTAAAG

ANAGA.AACCA

GCAGCGAAAG

AAAAAGCCGG CGTTTTATCA CAAAAGATTC ATTGGATAAA AACAAACAGG TGATTTATCT CAGAAGATTT AGGTGAAATT ATTCTCGGC AGCGGCCAAA CCAAAGATGT TCCTGAAAAA ACCTAAAAAA ACAATTTAAA

GGGTAA

EF091-4 (SEQ ID NO:348) SNQTDDLK NKAQDYTDYA VQKGTELTEI AKQKAGVLSD QASDLAGSVK E-KTKDSLDKA QCVSGDMLDN FKKQTGDLSD QFKKAADDAQ DHAEDLGEIA EDAAEDIYID VKDSAAAAKE TVSAGVDEAX ETI'DVPEKA AEAXEDVKDA AXDVKKEFKG EF092-1 (SEQ ID NO:349) 0* 0

TAAGGGGATG

AGCACGAGTT

GACATCTITG

GTTTTACTT

CCAAAAGGGA

AAAGTTTTAG

AAGAAAAAAT

TAT1'AGTAAC

CAGTATCGGC

TAGGTCCACA

TTCCTTTAGA

ATCAAGCAAT

GGCAAAAAAA

AAAAATGCAA

TTCTGAACCA

AGTTCGTTTC

TGTAATTAAC

CTCATTAATG

ACAATTATGT

AAAGCAGCAG

GATACAAACT

ATGAAAGGGC

ATGGCACA'T

GGATAA

TAG=1GTTC

AAGATCGTGG

TGGAAAATAA

AATTGAACA

ATGGCATGAT

CGCAGGAATG

CATGGAAGCA

AGAGGTGAAT

AAAATTACAA

GAATGGCGAA

0000 0 0000 00 0 00 00 EF092-2 (SEQ ID NO:350) MAKKT IMLVCSACMS TSLLVTKMQK AAEDRGMEAD IFAVSASEAD TNLENKEVNV 185 TABLE I. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

LLLGPQVRFN KGQFEQKLQP KG IPLDVINM ADYGKMNGEX VLCQAISLMG EF092-3 (SEQ 10 NO:351) AG AAGATCGTGG CATGCAAGCA GACATCTT-TG CAGTATCGGC 'TCAAGCA GT=TACTTT TAGGTCCACA AGTTCGTI'TC CCAAAAGGGA TTCCTI'TAGA TGTAATI'AAC AAAGTTTTAG ATCAAGCAAT CTCAITAATG

GATACAAACT

ATGAAAGGGC

ATGGCAGATT

GGAT

TGGAAAATAA

ATTAACA

ATGGCATGAT

AGAG'CTGAAT

AAAATTACAA

GAATGGCGAA

EF092-4 (SEQ ID NO:352) EDRGMEAD IFAVSASE-AD TNLENKEVNV LLLGPQVRFM KGQFEQKLQP KGIPLDVINM ADYGD2NGEK VLCQAISLMG EF093-1 (SEQ ID NO:353)

TAGTTFITT

TTCACGGCCA

CGTCAGTTAA

TTTCA'IIAT

CTCGTATACG

GTTTTXIATAA

AAAGCGGAAA

CCGATAAAGG

TGGCCATTGT

CACAAAAATT

TTCTTGCACA

CGCAAGTGAC

ACAAATCTGG3

CAATACTGGA

CAGAATTTTA

TTATTTGATG

TACGC:AAGCT

TOTTAAACAA

CCTCGATTAT

TCGAAA.ATAC

ATAG

ATGAGGCAAA

AGTTTTCTCG

ACCCAGGAAT

A.ATAGACGAA

ACATACAAAA

CAATATCAAG

AATA7CAGG

CCCTTCAGTT

ACTATGCAGG

AGTTAAAAAC

ATGAACAATT~

AGAGAGTTTC

AAACTTATTG

ACTAGAAGA.A

GAAAAGTATC

AGAAGAAAGG

AAGAATAACT

TCATCAAAAA

EF093-2 (SEQ ID NO:354) M RQKYSGNLLF TAMA.IVYLMS FLALQLLEER QLTQKFTQAT QEYYAGKS IF HLFLADVKQN RRKLKTEERL VYAQVTLDYT YKNEQLR ITV LLNKSGRKYQ YQERVSHQKK

AETILE

EF093-3 (SEQ ID 00 9. 6 @0 00 0 0 @0 00 S S 00 00 0S 0 0 @0 @0 0

S

CCTTrCAGrr ACTAGAAGAA CGTCAGTIAA CAcAAAAATT TACGCAAGCT TrTCAITPTAT TTCTIGCAGA TTAAACAA CTCGTATACG CGCAAGTGAC CCTCGATTAT GTTTATT AA ACAAATCTGG TCGAAAATAC AAAGCGGAAA CAATACTGG

ACCCAGGAAT

ALATAGACGAA

ACATACAAAA

CAATATCAAG

ACTATGCAGG

AGTTAAAAAC

ATIGAACAATT

AGAr-AG ITTC

GAAAAGTATC

AGAAGAAAGG

AAGAATAACT

TCATCAAAAA

EF093-4 (SEQ ID NO:356) 000 0 ::96.

0.*.0 LQLLEER QLTQKFTQAT QEYYAGKSIF HLFLA-DVKQN RRKLKTEERL VYAQVTLDYT

AETI

YKNEQLRITV LLNKSGRKYQ YQERVSHQKK EF094-1 (SEQ ID NO:357)

TAAACATTTG

ATTAATTATA

TTAAGTCAAA

CACCCTCAAG

TTI'AGCGATG

GCAAAACAAA

AGACA'ITCAG

TGAAAAAAAC

CAATTGGCGG

AGGTAACAAT

GCAGCACGCT

CAGTATTTTG

AGGTGAATGT CTCTTTTTrA AACATTTAAA AAITGGTCGT AACGATTGGT CCTACGATTG TCATTATGAC GTAAGTAAAC CTCAGAACCT ACTACGTCAT TATTGAACCA GGTGTTAGTA

TTACTCAAAA

TATTTr.CGAC

CTTTTGCCCGA

TGTATGAAGT

TATATGCAGA

TTCCAACAGA

ACGAAAGGGG

TTTGGCTCTA

TGAAATTACT

TGACGGAACT

ATACAATGGT

AGTCACGCAC

186 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

GGTTATCAGA

TGGGAAAAGG

GAAGTGAACG

AAATCTATTG

CATAATACCA

TTAAATTI'AT

ATTGGGAATC

AAAAAATCAG

ATGGCTGGTG

GGTTCTTTAA

TTCCATTTAG

A=ICTATTT

CCAGATCCTT

A=TCCATGA

GTAGAAACAC

ATTCGTAAAG

CGTGCGGAA-A

GAAACTAAAT

TATGCCAATC

ACTGGGGAAA

GCTGAGTITA

TGGAGTGAAG

ACTTTGGCTT

FI'CTGGCAAG

TCCATCAAAA

AAAGAACAAG

ACTGCCGAAA

GAAATCACAG

GGCTATGGTA

CCAGAAGGAT

GACCACTATG

ATTAAGATGG

AACCGTTTGA

TGGAAACACG

AGATATAACT

GAAGCCACAA

ACAACCGCAA

ACCGCTGAAC

GTAGCCTTTG

CAAACCr-TCA

GATGGTTCC

ACCCATGATG

CCAGATGGTA

AAkAGAATTTA

ACTAAGTTTA

AATGAAGATT

AGTACGCCAA

ACAGTGAAGA

TTTATCTTCA

AAAACCCTTT

CTGGAACAGA

GTTATA.AACT

AAGGAAAAAT

CTGTAAAAAC

CTGAGTTTGA

AATTAGTGCT

CTCGTACTGG

CGCTTGATAA

AGATCAAAAA

ATTMI'GGGAA

TGGATGGAAT

ATATGATTGA

CTTCGAAAAA

CTACTGATCT

ACAGCCCAGC

CACCA.AAAGA

CTAGTAATGG

AAACCGTGC

CCACTTI'GAC

AAGGAGCTGA

CTTTTAAAAC

TAGATGAAAA

AA.ACCAAAGC

AAGTTGATAA

TTATTCGCTT

CTGGATTTAA

GTCCTGAAGA

AGTTTGAAAA

TTCAAAAGAT

CGAAGACTGA

TGACCGT1'CC

TGCTTTATGA

GAAATAAAAA

TGCATGAAAT

AAGCTG3CCCA

CGTTGGCGAA

AAG~TGA

AAGCAGGCAA

ATTGTACGAT

A.AACTTTTAC

TACTGGATGG

CGAACAAAGA

CCAAAACCGT

CTF'TTACGGA

TGAAAGAAAA

AACAACCCGA

CATTCCCGCA

TTTTCGAC

GCCATCAATG

TATTGATACA

CCATTCCATA

TAATAAGGCA

AAT1'TTAGGT

TAAAGTCGTC

TACTCCAAAC

AACTCCAGTC

GCCCAATACC

AATCGATAAA

AGCT

T

ITACCT

TCCCCATGGT

TACCACACCC

TATGCGACAA

TTGGAATOAC

TGGTGAAATT

GCTTGCTAAT

TTT'CGTGAAT

TCTTGTTACC

AAAAGAAGAC

ATATACTCTC

AGAATTAGTG

GAACCAAGT

ACCTGAAGGA

TAACGAAAAA

TGGCTI'TGAT

CGACTTATCT

TAAAGCGACC

TCTTCCTTAT

'ACACCACTA

CTATGTCTT

TTACGAGAAA

TTTGCCCGAG

ATTGAATACC

CAAAGAAGAC

AGAAAAAGAC

CAAAGAGAAA

TAAAACCATC

TGAGCCAGtA

TGAACGCCAT

TCATGGTGAC

CTCAAAAGAA

AATTrGGA;A

ACTTGCGCAA

AATCAATTAC

ATCTCAAACC

AACACCAGCT

AACTGGGGAG

TGCTGGGTAT

TCTGATAAAG

AATATGGTTG

AAAAGATTAG

ATTGAGGAGT

CAATCGACAA

CAAAATACGG

TCTAAt'TCCA

GCTTATAAAA

TACGCTATTA

GAATCAGGTG

TCAAAAGATG

kCAAAGGTAA

ATGGCTGCCA

A.AAGGTCAAA

AATTATTCTC

GTCCA-AGAAA

GCITTTGGAAC

ACCITCAAAC

AGTAACGTA.A

AAAGATACCG

TTTACTGCA

AAGGGAACGA

GCCGTTAAAC

TATACTTG

AATGCCGTA

TTCTTTAAAT

TTTAAAGTGT

ACAr.CTTCTA GGGGATTATTr

GAAATCCGTT

ACCATTACCG

CTAACTAACA

AAAGAAGATA

CTTGATrTrTTA

TGGTATGTCG

GAAAAAGCCA

ACTGGAACTT

CTC'FrGTTCA

GCGAAGGAM

GTTTCCATCC

GTGATGGATA

GC'TrCGAAA

TCTGGCAAAA

AAAGTAGATA

GAAAALAGATO

TTAIACACCAA

GTTCCAAGTA

AAAAATTCCA

TATTTCTGGA

CGAAACTAGT

CACAAAAGAT

GTGC-TGCTTC

ATCAAAAAAA

CTTTAATAGA

CGAATATAGA

AATCAGGA.AC

AAGCAGGACT

AAAMrAATrCT

ATAFTGTACC

TGACAACAGA

CTATTACTGA

CCATTAAAGC I

TCTTT'AGA

TAGCTGGAAA

TAACAACQGA

TGGGAACCTA

CAACAAAAGT

AAGGCCAAAA

GTAATGAGAG

AAGATGGTCA

AAGCTTCTGA

ACCTAGCAAT

ATGAAACGAA

TTACTCGAGA

TTC:TGGATC

.CGCCATTGGA

ACGAOCAAT

TACTTAAGA

CTACATTTAA

AAGA.AGGACA

ACGAGITISC

GT=GACTTIC

CCGAGCTAGT

TAGCTCAAGC

AACCGGTGGT

GGAAAATTCT

ATTATCTGTA

CTAGCTTGAA

AAACAAAAGC

TGT=rATGA

CA-ATTCTGTA

TTGAGCATGA

CCGGAAAGTA

GAAACGTGAA

AAGA.AGTGCC

ATTCTCAAGA

ACGT1' ACT

ATCGCCGCAA

ATCGGTTCTT

GATTTGGGAA

AGTTGATATA

ACCAAG=TT

TAAAAATGAA

F1'ACCGTrCTA

ATTAACATTG

TCAAACTGTG

GGAAACTAAG

P.GAAACGGTT

TAAAGATGGG

AAAATCGGTG

GGGCGAGACC

GAAGACTGGG

TACATTTGCC

TGAAAAAGGT

TACGTGACA

CGAGTTAAA

CCAAGAAATT

TCAAGGGAAA

AGCTGITAAA

TGAAACAGTG

TAACGAGTAT

GTATCCTGTA

T'CTTACGCCA

G'-CTGATGGC

AGGGACCA.AN

AGCG'rTTGAT

AATAGAGGCT

GGAAAACAAA

AAAACAACCA

TG LTAGTCTC

TCTTCGACT

TGATAAATTG

CATTGATGTG

GATTGCCCAA

GCATAAATTA

TGAAAACAAG

CAATCAAGCA

CCACCTAGAA

TGTGTCGGTT

TCTTTACTA

AGTGAATGAT

TCCAGAAGGA

TGGAAAACAC

AACCATACCG

ATCTAGTCCC

G ITAGTTGGC

TTAA

EF094-2 (SEQ ID NO:358) MKKTTKN WSLFATLALL SQTICTIGP TIAFADEITH PQEVTI HYDV SKLYEVDCTF SDGSTLSERT TSLYAEYNCA KQTVFCIEPG VSIPTEVTHG 187 TABLE 1. Nuclcotide and Amino Acid Seqeuences; of E. faecalis Genes.

YQKNPLPSMS

SIEGKINKAI

GNQLVLTPNS

SLKIKKIDKE

DPYMIDTTPM

RKDSPAGEIV

ANQTVALVTS

SEAFKTELVK

IKKVDNNEKN

ITGAEDKATT

DYAKSEYVFT

KDGM(KLNTL

TATLANKEKT

TVNCTIERHV

OGTNKE IWKS

EDLKEKSQTL

ILIFSTAGYY

DKAKLVSVLW

EEYQKKPSFH

NSKSGTLTLK

SGDIVPETVF

AATIKAGETI

QEITTDEKGR

NVXGQNQEIT

GTKASDETVT

AVITRDVTAK

ACNEQLGFDG

ITEEGQKQP I

DFTELVDKLR

GTWKILHKLT

S IQTKAHLED

GKIEHEVNDK

TPKEVPTIPS

FWNRRN

EKAGTDIDTN

NTTVKTILGQ

KSAGTGTPVA

HLDFGKALPS

SMTSKNMRQK

AE:TPFLANA

GETTLTKEDK

LALDEKNQVA

EQVIRFGFDF

YGKFENtJPYG KM4VTVPYEKL YNLHE IKEDW AEQVLDKS IV

GSQTFTHGDV

EFTKTTLAEI<

TPKQPT-TPAV

MVAQKMIWEE

STTLI DKDEL

YKKAGLQTVM

KDV'ITDKDGI

GO ILLEKTGV

LELCTYYVTE

DTGNESQGKA

VKHLAI NEYF

FKFAG-SADGT

DYLLEE I EAP

TNNEFSVSLN

YVIVAQAIDVE

LFNWVYENKV

MDMFDD VS VT

VDTGKYPEGT

PSNSQESSPT

V7NGYF.LHS IK

NELSEFDKVVQ

AGALDKPNTY

SILDGIPHGT

ETGTDLWND)N

TKS SNGFVNT

EFKGAEYTLF

WQETXAPEGY

AETGFNDLSF

EGFQKITPLE

RLMLYDLPEK

ATKAAQEKDE

AFEAGNEPVA

HDVLDGSKEA

KFTFTE INYE

VKTFPQTGEK

RLGGASVDIK

NTANIDYRVI

AIKINVETKG

KVTITEKSVP

YSLAGNTIFA I

FKPTKVELKY

TAKDGQAVKW

TLDETKYPVS

KVSPLEGTXE

IRSTFKENKD

EDSLTSLATW

KAKPVVIAET

KDASLNNQAQ

FETI LYALLP

KDGNVNGKHN

NSNVLLLVGF

EF094-3 (SEQ ID NO:359) CGA TG-AAATTACT CACCCTCAAG AGGTAACAAT TTTAGCGATG GCAGCACGCT GCAAAACAAA CAGTATFITG GGTTATCAGA AAAACCCTTT TGGGAAAAGG CTGGAACAGA GAAGTGAACG GTTATAAACT AAATCTATTIG AAGGAAAAAT CATAATACCA CTGTAAAAAC TTAAATTTAT CTGAGTITGA ATTGGGAATC AATTAGTGCT AAAAAATCAG CTGGTACTGG ATGGCTGGTG CGCTTGATAA GGTTCTI'TAA AGATCAAAAA TTCCATTTAG ATI1'GGGAA ATTTCTATI'T TGGATGGAAT CCAGATCCTI' ATATGATTGA ATTTCCATGA CTTCGAAAAA GTAGAAACAG GTACTGATCT AT1'CGTAAAG ACAGCCCAGC CGTGCGGAAA CACCAAAACA GAAACTAAAT CTAGTAATGG TATG;CCAATC AAACCGTGGC ACTGGGGAAA CCACTTTGAC GCTGAGTTTA AAGGAGCTGA TGGAGTGAAG CT'ITTAAAAC

TCAITATGAC

CTCAGAACGT

TATTGAACCA

GCCATCA-ATG

TA'FrGATACA

CCATTCCATA

TAATAAGGCA

AMTTAGGT

TAAAGTCGTC

TACTCCAAAC

AACTCCAGTC

GCCCAATACC

AATCGATAAA

AGCTTTACCT

TCCCCATGGT

TACCACACCC

TATIGCGACAA

TTGGAATGAC

TGGTGAAAT

GCTTGCTAAT

T=rGTGAAT TCTrG'rrACC

~AAAAGAAGAC

ATATACTCTC

AGA.ATTAGTG

GTAAGTAAAC

ACTACGTCAT

GGTGTTAGTA

TCTGATAAAG

AATATGGTI'G

AAAAGATTAG

ATTGAGGAGT

CAATCGACAA

CAAAkATACGG

TCTAATTCCA

GCTTATAAAA

TACGCTATTA

GAATCAGGTG

TCAAAAGATG

ACAAAGGTAA

ATGGCTGCCA

AAAGGTCAA.A

AATTATTCTC

GTCCAAGAAA

GCT'ITGGAAC

ACCTTCAAAC

AGTAACGTAA

AAAGATACCG

TTTACTGCAA

AAGGGAACGA

TGTATCAAGT

TATATGCAGA

TTCCAACAGA

CGAAACTAGT

CACAAAAGAT

GTGGTGC'rC

ATCAAAAAAA

CITTAATAGA

CGAATATAGA

AATCAGGAAC

AAGCAGGACT

AAATTAATGT

ATATTGTACC

TGACAACAGA

CTAT'TACTGA

TGACGGAACT

ATACAATGGT

AGTGACGCAC

ATCGGTTCTT

GATTTGGGAA

AGTTGATATA

ACCAAGTTTT

TAAAAATGAA

TTACCGTGTA

ATTAACATTG

TCAAACTGTG

GGAAACTAAG

AGAAACGGTT

TAAAGATGGG

AAAATCGGTG

CCATTAAAGC GGGCGAGACC TTCTITAGA GAAGACTGG TAGCTGGAAA TACATrGCC TAACAACGGA TGAAAAAGGT TGGGAACCTA TTACGTGACA CAACAAAAGT CGAGITAAAA AAGC-GCAAAA CCAAGAAATT GTA.ATCGAGAG TCAAGGGAAA AAGATGGTCA AGCTGTI'AA-A AAGCTTCTGA TGAAACAG EF094-4 (SEQ ID NO:360)

DEITH

PQEVTIHYDV

YQKNPLPSMS

SIEGKINKAI

GNQLVLTPNS

SKLYEVDGTF

DKAKLVSVLW

EEYQKKPSFH

NSKSGTLTLK

SDGSTLSERT

EKAGTDIDTN

NTTIVKTILGQ

KSAGTGTPVA

TSLYAE:YNCA

MVAQKMI WEE STTLI DKNEL

YKKAGLQTVM

KQ'IVFCIEPG VSIPTEVTHG VNGYKLHS 1K RLGGASVDIK NLSEFDKVVQ NTAN IDYRVI AGALDKPNIY AIKINVETKG 188 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faccalis Genes.

SLKIXKKIDKE

DPYMIDIrPM

RKDSPAGEIV

ANQ IVALVTS

SEAFKTELVK

SGD IVPETVF

AATIKAGETI

QEITTDEKGR

NVKGQNQEIT

GTKASDET

HLDFGKALPS

SMTSKNMRQK

AETPKELkNA GET1'LTKEDK

KDVTTDKDGI

GQILLEI<TGV

LELGTYYVTE

DTGNESQGKA

S ILDGT PHGT ETGTDLWtDN

TKSSNGFVNT

EFKGAEYTLF

KVTITEKSVP

YSLAGNTFAI

FKPTKVELKY

TAKIDGQAVKW

EF095-1 (SEQ ID NO:361) a a a

TAAGAATTGT

GAArI'CATAG

GTTTI'TGCAG

ACAGCAGAAA

ACACTGGCAT

ATTAGAGAAA

GGGCAGCCAG

CCAGATGGCA

AAT'1AAAAG

GTGCTTGCAG

TACAATAAGA

GGAAACCAAT

TATAGTCAGA

TTGTACAACC

Trr1CAGGGC

ACTCAAGGGA

GAGCGTGACC

TCAAAAACGT

AAAAGCGTAA

TATAAGGAGA

CCACCATCTC

TTAGCTGGAA

GGTTGGTATC

TCCCCTGTTT

GCTGAAATGC

CAGATTATGC

AGTGGTTGGT

CCAAACAAAA

GAAGTGCCTA

CATCAAGTGT

GATACTGTCA

ATCAGCACAC

GGTTTAAAC

AAAAAATCAC

GATCAACTAT

CAGTACAATC

GTTGCCAACC

TATCAATTTG

AAACATCAAA

TGGATTGTTC

TAACGGGCAT

AAACATTACC

AAGCCGAAAG

TGTCAAAAAG

GAATTGAGAC

TAAACGCCAA

TAAATGTGTG

AAGTGGTAAT

CGAGTAATCA

AAGGGGAAAT

ATCCAACAAC

AAACAGGAGT

AAGTGAAGGT

CGGT=ATTA

AACCAATCCC

CTTACACCTT

ATCAATTTCA

CGCCCAGTTA

TACCTCAAAA

ATTTTATTCA

AAAAACTGCC

AAGATAAAAC

TTAATGAAAT

AAATAGAAGG

7ACAAATGT

CACCAGGTCT

TTGTTCCTAT

TTGGTCAGAC

TACAAGCGC

GAATCCAACC

CAACTTGA

AGGCACCACA

AACCCAATTG

CATCAATGAC

AACCAACGCA

GTGTAGCTAG

ATTTCTTT

CTTATCAAGC

TTTAGAAAGA

CTGCCATATA

AAGTACAAAA

TCAACAACCA

TGAGTTAATC

GCCTAACCTA

TGAGATCCTT

GGAAGGTGAA

TCCAAGTGAG

AACA=''TT

TCATCCCAAT

AATTTCGCAA

AACGTTTAAC

TGATTCATCG

TCATGTTACC

TCCACCACCG

TAAACAGAAA

AGGATGGTAT

TGATATTACC

'AAAITATACA

GGATCACCAA

ACAACAATAC

NAAACAAGAG

GAACGCTA IT

ACTAGTCAAA

GGGAGAAGTA

AGCTCGGCCA

TACTGATGAA

AGCAACTATT

TGTTGAAATG

TAAAAATCAA

T'TI'GGCAAA

TTATTATGAA

GGCACTAACT

AAAG TATTG

AACTAAAGTT

CCATAFI'GTT

TrGATCAA.ATC

AATGGTGACT

AGGGACAATA

TTAGTATTCC

CMGTAAGAG

CACACAAAGG

GATAATGAGG

ACTTATCGTr

CTACAGTATC

AGTCAACCAG

AA.AGTAGCCG

TTACCAAGAT

TATCCGCTGC

TTTGAATTGG

ATTAGCGAGA

AATCAATCTG

AATCGCAAAG

GGGTTTAGAC

CATCTTTTGC

AAAGGGAAAA

TATGACGACA

TTTGAGGAIM

CAACCAATAA

AGCGTTAACG

AGCTTAGAAA

ACAGCAGL=

GTCATGCCAA

CCGTTAAAAA

ATCCAAGTCA

AATTIGGCGAG

ATGATGACAA

A.ATGAANT

GAAATTGTGG

GTCGCCAM

AATCTCACG

GCAGAAC TAT

TTAGGAACAA

GCTCTTGGCA

GCCAATGGTC

AAATTAGAAA

TGGAAT'TTAG

TGAAGCGAAC

:CATAcTAAT.

AAGGAACCAA

ATAAACTACA

CTAATGTTAC

ATGGA!TI'AT

ATAGTTGGCA

TGACAGCATC

TCTATTCC-GA

ATTATACTTC

CA.ACTATTTC

AAAAAATGTC

GTCAAAAACT

GGCTMAA

TGACAGAACA

AAGGAAAGCA

CAAGTAGCTA

CGAA.ACCTGA

ATGATGATTT

TCAATGGTGT

CTACGGATGG

GTAAAACI'A

AAACGAAGCG

ATAALGGAAAT

GTGGTI'ATAT

AAATAAACT

CGATTCGTGT

TTGOCATTAC

CAATTrGCTAC

TTTCMCTGT

CACCAGATGA

CTAGCAACAC

AA.AATCCATA

CCCCCTTTGA

CCAATGMTC

AAACAACCGC

AGTIrMACGA

TTCCAGCAAA

TGACAGGCCC

TAAATGGAAA

ACAGACAACT

TCATTCATI'A

TGATGPLAGAA

AAGTCAAACG.

TAATGAAGAG

AGGCAATTCC

TACAGCCT

CATGTCAACG

TTTAAGCTTA

CGACGCATCA

TGCACAACAA

AATCGTTCCT

TTACTTTAAA

=ITTGGAT

AACACTTATT

TGAAATTGAC

AAATTIAGAA

AACTGTTGTC

TGA.AATTGCA

CTTTCGCTAT

TTTATATCAA

ACCCATAALAC

AACTGCAAAA

ACAAATTTG

AAAGCCAGCA

TGGATCTGAA

TTTAAATACG

AGGTGAACCA

TCACGCAGCT

GGAAGGTI=

GCAGCAACAT

'PITACGTTTG

AGGAAGAGTG

AGGTTTTATT

TATTcAATTA AAGTGATmTT

TCAAGGTAGA

ATAA

EF095-2 (SEQ ID N'O:362) MKRSKWKE LIVTGICHIL VFPILIQTTV FAETLPSTKQ VRE-GTNHSLT AEKAESEQPQ TKDKLHDEET LALSKSELID NEANVTSQTI RERIETPNLT YRYGFUIEEG QPVNANE ILL QYHSWQGNS P DCINVWEGES QPVTAsTVAN LKEVVIPSEK VAVYSDMSTV 189 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

LAASNQTFFL

SQKTGVTFNI

QGKPIPPPPG

S VT PSY DITY

AGKKLPQQYS

EMQ lEG LVKV NKIVP ITfEN

TVRIQPKNQE

KSQPNWALTA

ANGVASHIVA

PRYYTSLSLY

SESQKLIVPL

FRQGKQTLIE

DDNDDLTVVY

VNGKTYLYQG

MPSGYIQIWQ

WRVGITLNTE-

IVAPDEEGF I

ELSPFEGRVD

NGQFCESDVY

NKKGEIDPNY

YNQVKVDS SN

RDPYTFKQKD

KEI PQKNYTF

WYQDKTKQES

IMLTNVGEVP

VPIGQTATIM

STPTFDFGKV

QLSSMTKLLL

QFDFSFDQIK

PLPTISDASG

QSGLLNYPKF

LLPSSYEIEDS

EDVNGVE lAP LEKTKRP INS LKK INLKPAS

MTTIATGEPD

AISSNTQQHG

GTTNVSGFIQ

L I PANQGRK

NQYPTTISQF

SGPVVYYHVTN

KTYQFQGWYK

PSDF IQCHQQ

PVFN&ENAIT

GWSPGLARP I

QVLQAMVEI~

LKQAADYYEN

YNQPTETKVA

DQTYQAMVTW

ELEKMSAQQY

RKVTEHFVDT

GKTKPENLEK

P I'TDGFRYL AVYKE ITAKA

QVTIRVGSEP

GNFSAVHAAD

GQENPYLRLK

LGKTTAIQLV

NLVTGP

EF095-3 (SEQ ID NO:363) AAGTACAAA.A CAAGTAAGAG 4* a.

ACAGCAGAAA

ACACTGGCAT

ATTAGAGAAA

GGGCAGCCAG

CCAGATGGCA

AA'ITTAAAAG

GTGCTTGCAG

TACAATAAGA

GGAAACCAAT

TATAGTCAGA

TTGTACAACC

TTTTCAGGGC

ACTCAAGGGA

GAGCGTGACC

TCAAAAACGT

AAAAGCGTAA

TATAAGGAGA

CCACCATCTG

TI'AGCTGGAA

GGTTC-GTATC

TCCCCTGTTT

GCTGAAATGC

CAGATTATGC

AGTGGTIGGT

CCAAACAAAA

GAAGTGCCTA

GATCAAGTGT

GATACTGTCA

ATCAGCACAC

GGTITAAAGC

AAAAAATCAC

GATCAACTAT

CAGTACAATC

GTTGCCAACG

TATCAATTTG

AAAGATCAAA

AAGCCGAAAG

TGTCAAAAAG

GAATTGAGAC

TAAACGCCAA

TAAATGTGTG

AAGTGGTAAT

CGAGTAATCA

AAGGGGAAAT

ATCCAACAAC

AAACAGGAGT

AAGTGAAGGT

CGGTTATTA

AACCAATCCC

CTI'ACACCTT

ATCAATTCA

CGCCCAGTTA

TACCTCAAAA

AT=-k~TTCA

AAAAACTGCC

AAGATAAAAC

TTAATGAAAT

AAATAGAAGG

TTACAAATGT

CACCAGGTCT

TTGTTCCTAT

TI'GGTCAGAC

TACAAGCGGC

GAATCCAACC

CAACTTTTGA

AGGCAGCAGA

AACCCAATTG

CATCAATGAC

AACCAACGGA

GTGTAGCTAG

ATTTTTCT'rr

CTTATCAAGC

AAGGAACCAA

TGAACAAC-CA

TGAGTTAATC

GCCTAACCTA

TGAGATCCTT

GGAAGGTGAA

TCCAAGTGAG

AACATITTT

TGATCCCAAT

AATTCGCAA

AACGTITAAC

TGATTCATCG-

TCATGTrACC

TCCACCACCG

TAAACAGAAA

AGGATGGTAT

TGATATTACC

AAATTATACA

GGATCACCAA

ACAACAATAC

NAAACAAGAG

GAACGCTATT

ACTAGTCAA

GOCAGAAGTA

AGCTCGGCCA

TACTGATGAA

AGCAACTATT

TGTTGAAATG

TAAAAATCAA

TTTTGGCAAA

TTATTATGAA

GGCACTAACT

AAAGTI'ATTG

AACTAAACTI

CCATATTGTIl

TGATCAAATC

AATGGTGAC'I

TCATTCATTA

CAGACAAAGG

GATAATGAGG

ACTTATCGTI

CTACAGTATC

AGTCA.ACCAG

AAAGTAGCCG

TTACCAAGAT

TATCCGCTGC

TTTGAAIG

ATTAGCGAGA

AATCAATCTG

AATCGCAAAG

GGGTTTAGAC

GATCTTMTC

AAAGGGAAAA

TATGACGACA

TTTGAGGATr,

CAACCAATAA

AGCGrtTAACG

AGCTTAGA;A

ACAGCACTOT

GTCATGCCAA

CCGTrAAAAA

ATCCAAGTCA

AATTGGCGAG

ATGATGACAA

AATGGAAATT

GAAATTGTGG

GTCGCCAT

AATGrGTCAGG

GCAGAACTAT

TTAGGAACA.A

GCTCTTGCCA

GCCAATGGTC

AAATTAGAAA

TGGAATTTAG

ATAAACTACA TGATGAAGAA CTAATOTTAC AAGTCAAACG ATGGArTTAT TAATGAAGAG ATAGTTGGCA AGGCAATTCC TGACAGCATC TACAGTGGCT TCTATTCCGA CATGTCAACG ATTATACTTC TTTAAGCTTA CAACTATTTC CGACGCATCA AAAAAATGTC TGCACAACAA GTCAAAAACT AATCGTTCCT GGCTATTGAA TTACTTTAAA TGACAGAACA TI'TTGTGGAT AAGGAAAGCA AACACTTATT CAAGTAGCTA TGAAATTGAC CGAAACCTGA AAATTTAGAA ATGATGATTT AACTGTTGTC TCAALTGGTGT TGAAATTG-CA CTACGGATGG CTTTCGCTAT GTAAAACTTA FTTATATCAA AAACGAAGCG ACCCATAAAC ATAAGGAAAT AACTGCAAAA GTGGTTATAT ACAAATTTGG AAATAAACTT AAAGCCAGCA CGATTCGTGT TGGATCTGAA TTGGC:ATTAC TTrAAATACG CAATTGCTAC AGGTGAACCA T'ITCTOCTGT TCACGCAGCT CACCAGATGA GGAAGGT=I CTAGCAACAC CCAGCAACAT AAAATCCATA TrrACGTTTG CCCCCTTTGA AGGAAGAGTG CCAATGTTTC AGGTTTATT AAACAACCGC TATrCAATTA AGTFTGACGA AAGTGATGT TTCCAGCAAA TCAAGGTAGA TGACAGGCCC A a EF095-4 (SEQ ID NO:364) STKQ VREGTNHSLT 190 TABLE 1. Nucleotide and Amino Acid Seqeuences ofrE. faecalis Genes.

AEKAESEQPQ

QPVNANEILL

LAASNQTFFL

SQKTGVTEFNI

QGKPIPPPPG

SVTPSYDITY

AGKKLPQQYS

EMQIrEGLVKV

NKIVPITDEN

TVRIQPKNQE

KSQPNWALTA

ANGVASH IVA

TKDKLHDEET

QYHSWQGNSP

PRYYTSLSLY

SESQKLIVPL

FRQGKQTLIE

DDNDDLTVVY

VNGKTYLYQG

MPSGYIQIWQ

WRVG ITLNTE

IVAPDEEGFI

ELSPFEGRVD

NGQFIJESDVY

LALSKSELID

DC INVWECES

NKKGEIDPNY

YNQVKVDSSN

RDPYTFKQKD

KEI PQKNYTF

WYQDKTI<QES

IMLTNVGEVP

VP IGQTATI2

STPTFDFGKV

QLSSMTKLLL

QFDFSFDQIK

NEANVTSQTI

QPVTASTVAN

PLPTISDASG

QSGLEJNYFKF

LLPSSYEIDS

EDVNGVEIAP

LEXTKRP INS

LKKINLKPAS

MTTIATGEPD

Al SSNTQQHG

GTTNVSGFIQ

fEI PANQCRK

REPRIETPNLT

LREVVIPSEK

NQYPTTI SQF

SGPVYYHVTN

KTYQFQGWYK

PSDFIQDHQQ

PVFNENAIT

GWS PGLARP I

QVLQAAVEMN

LKQAADYYEN

YNQPTETKVA

DQTYQAMVTW

YRYGFINEEG

VA'\VYSDMSTV

ELEK14SAQQY

RKVTEHFVDT

GKTKPENLEK

P ITTDGFRYL

AVYKEITAKA

QVTIRVGSEP

GNFSAVHAAD

GQENPYLRLK

LGKTTAIQLV

NLVTGP

EF096-1 (SEQ ID NO:365)

TGAGGTGGCC

ATTGTCATCT

GATACCTTGA

GAAAAAGAAA

ACAAAAATTC

CAAAAAATGA

GAAAACATTG

CCGTACTTCT

ATCCAACATT

GGCCCTCG-CG

AACGACCAAC

GCAATTGTTG

ACFI'CTCTG

AAGTTAAAAT

TGTTI'TTGG

CCATPI'ACA-A

CAGGCTATAA

AGCAAGGTGG

TGAAAGCGAA

ATGCACGCTT

GGGGCACGTI'

GGATOATTI'

AAGTG PIAGG

AATTACGTCA

CCGAT1GAAAT

GTGAAGCTGC

GAAAAAATTA

CGTGCGCCAA

TTGGGGGGAC

AGTCAA'ITAC

CACAGCCTAT

GATGCTTTTA

1TTAGATCAA

GGGGATTAT

ATCGCGCCCG

ATTATCTT'

GGCTACCGAT

CAAAATGTAC

TGAAATGCTA

CTGGTTTGAT

TATGAACTT

CACACCAAAT

TTATCCAGAT

CTTAGGAAT

CAGTCACTTT

TTGAGAAAG

TATATAGATC

GAAACCTTTG

GATATTGCCA

CCACTTGATC

TCCTTTGATC

TATAATGATA

GAATTAAAAA

AACAGTTTAG

AAGTI'AAACC

ATGGCTAATG

GAAAACAATG

AACATTGT6A

ATGTI'ACGAC

AAAGAAGCTA

GATGAAACTA

TATAACGATC

TTAT7GGAAT TATCGCTATT CAAGTGGCAT GG^CAGGAGCA CCGCCTTGAT TAAGAAATTT AFTCTAATGA AGCTATGTAT TTCCTTCTGA ATATATGATT ACAGCAAATT AAAAGGC FTA CCAAAAATAA GTTTTCCGTT AAI'TATTGA CGGCCGTCAG ATAATGTCAT GCTGATTGAT GCTATTCGTT AA.ACAGTAAA GATTAACGAkA CAATGTCAAA AAGAAAGTGC AGTTGCTGTA AACATCTACA TTATGTGAT TGCCTAAGAC AGCCAAAAAT CAGAAAATGC GGCACAAAAT AAA.AACTATT ACCAAAAGA-A TCAAACATTT AGAAGTTTAC TGTTCTTGGA GT=TAAGATG 4 CCCAGTGAAG GCTCTAATCT AAAGAGGGTG CCTATGCATT GCAGAATATA TTGGTTATTC GTTGCCGAAG ATAAACAATT CAAGACTTAG GTCAAGAATA TATCGGAAAT AA EF096-2 (SEQ ID NO:366) MKKLQ SLFIGIIAII VILFFGVRQL EKASGMAGAD TLTIYNWGCY IDPALIKXFE KETYKVNYE TFDSNEAMYT KIQQGGTAYD IAIPSEYMIQ KMMKAKMLLP LDHSKLKGLE NIDARFLDQS FDPKNI(FSVP YFWGTLGIIY NDKFIDGRQI QHWDDLWRPE LKNNVMLIDG AREVLGLSLN SLGYSLNSKN DQQLRQATDK LNRLTNNVKA IVAD)EIKMYM ANEESAVAVT FSGEAAEMLE NNEHELMYVIP SEGSNLWFDN IVMPKTAKNK 'EGAYAFMNFM LRPENAAQNA EYIGYSTPNK E.AKXLLPKEV AEDKQF'YPDD ETIKHLEVYQ DLGQEYLGIY NDLFLEFKMY

RK

EF096-3 (SEQ ID NO:367) AAGTGGCAT GGCAGGAGCA GATACCTTGA CCATTTACAA GAAAAAGAAA CAGGCTATAA ACAAAAATTC AGCAAGGTGG CAAAAAATGA TGAAAGCGAA GAAAACATI'G ATGCACGCTT CCGTACTTCT GGGGCACGTT

TTGGGGGGAC

AGTCAAITAC

CACAGCCTAT

GATGCT'TA

TTTAGATCAA

GGGGATTAT'r

TATATAGATC

GAAACCTTTG

GATATTGCCA

CCACTTGATC

TCCTTTGATC

TATAATGATA

CGGCCrI'GAT A~rCTAATGA TTCCTnTCTGA

ACAGCAAAT

CCAAAAATAA

AATTITATTGA

TAAGAAATTT

AGCTATGTAT

ATATATGATT

AAAAGGCTTA

GTTTTCCGTT

CGCCTCAG

191 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.f[aecalis Genes.

ATCCAACATT

GGCGCTCGCG

AACGACCAAC

GCA.ATTGTTG

ACTTTCTCTG

CCCAGTGAAG

AAAGAGGGTG

GCAGAATATA

GTTGCCGAAG

CAAGACTTAG

GGGATGA=T

AAGTGTTAGG

AATTACGTCA

CCGATGAAAT

GTGAAGCTC

GCTCTAATCT

CCTATGCATT

TTGGTTATTC

ATAA.ACAATT

GTCA.AGAATA

ATGGCGCCCG

ATTATCTTTG

GGCTACCGAT

CAAAATGTAC

TCAAATGCTA

CTGGT FGAT TATGAAC Tr

CACACCAAAT

TTATCCAGAT

CTI'AGGAATT

GAATTAAAAA

AACACTTTAG

AAGTTAAACC

ATGGCTAATG

GAAAACAATG

AACATTGTGA

ATGTTACCAC

AAACAACCTA

GATGA-AACTA

TATAACGATC

ATAATGTCAT

GCTATTCGTT

GArI'AACGAA

AAGAAAGTG.C

AACATCTACA

TGCCTAAGAC

CAGAAAATC

AAAAACTATT

TCAAACATT

GCTGATTGAT

AAACAGTAAA

CAATGTCAAA

AGTTGCTGTA

TTATGTGATT

AGCCAAAAAT

GCCACAAAAT

ACCAAAAGAA

AGAAGTTTAC

TGTTCTTGGA GTTTAAGATG

TATCGGAAA

EF096-4 (SEQ ID NO:3 68) SGMAGAD TLTIYNWGDY IDPALIKKFE KETGYKVNYE TFDSNEAMYT KIQQGGTAYD NIDARFLDQS FDPKNXFSVP YFWGTLGIIY AREVLGLSLN SLGYSLNSKN DQQLRQATDK FSGEAAEMLE NNEHLHYVIP SEGSNLWFDN EYIGYSTPNX EAKKLLPKEV AEDKQFYPD

RK

IAIPS EYMIQ NDKF IDGRQI

LNRLTNNV*XA

IVMPKTAKNK

ETIKHLEVYQ

KDOUK-kXMLLP

QHWDDLWRPE

IVA-DEIKMYM

EGAYAFIMFM

D)LGQEYLGIY

LDH SKLKGLE LKNNVMLI DG

ANEESAVAVT

LRPENAAQNA

NOLFLEFQ4Y EF097-1 (SEQ ID NO:369) *99 9 4*

TAGAAGTATT

ATGCATTCGC

GGTCATCGTT

TT1GTCTTTGG

ACGATAATGA

GAGGAACAAC

GATGTTCCAC

AAAATTGAAC

ITT7TrAGCAG

GCTGTTGAAA

CTTTTACCAT

ATTAACCATO

ATTTTATTTC

CTGTTTGGGC

GGGGGCATTC

GTAATTGCTG

GCTCCAGCTT

CTGGCGGTTI'

TTATTAAAAC

CAAGTGGAAA

GGCTCAAGTG

ATCCCTGTGA

ATTCAAGCAG

GTTCAAAATT

TCTTCTCAAG

ATACAGAAGC

GAATTATTGC

CTGGAAACAG

GCCTATCATT

AAAGAGTATC

CTAATTATCT

TCTTTTTTAA

TGAGTGGCAT

TGGCTGGCTA

TTCGAGTTGT

GTGGCGGCGT

AGTTGTTTGG

AAATTCTCTT

CAATTGTCGGG

GCGCTAGTTT

TGGTTCACGT

GCTTATTAAC

TATTGGAAAC

CTGTAGGACA

ATGSAAAFIA

GAGCAATGAG

CGCCAGGTI'C

TAGCGGAAT

GTCAACGAGG

CAGTCACACC

CCATGGGGGC

CTTACCAGTC

AATTGAAACA

TTTAGAA.AT

AGCAATCTTC

FrGTTTTTT

GGCAACAAGC

TCqTrTTTTAC

TAGATCTAAC

AAGAATGGCT

ACATAGAGAG

ACATAAGTTT

GATTATGCCA

TACGACTGGG

TTTACCGATT

TGTTGCTGCT

TGCTATCTTT

ACCGAAAGTT

AGGACTGCTG

TTGCCTGTAT

=~CTTAGAG

GCCTCTAGGT

AAACCCTGGA

ACGAAAAACA

TITT-CCGTTT

TGGTACGCTIT

ATTGGTTGCG

TIGTTAGC

AATTGAACCA

TAACTATCAG

TAGTTTGCTA

CG TTCATCAG

GITACCACAA

TAAATCCTAT

ACTTGG'-rCA

ATATGCCGAG

GGCGAAACAA

CAAGGAGACA

GCAACAAAAT

GGAAGGAGGA

CGAGGGACAA

GTGAAAGTAA

AATTTGAGTA

AATCTACGC

CTAArI'GGTT

ATTGCGACAG

ATTGGCCCTT

AAAGAAGGCT

TGCTGTTTTG

CAATTTTCTT

CCCTTAAAAG;

TTAGAAGGTG

CCAGGCGTGG

GCAGGAGGTG

GTTTTGATCG

GTTTTCAAA

ATTTTAGCCA

TTTCTGTGCT

GTTTCAATrGA CAAATTTrAT

AGCCGACAAT

ATGAAGTGGC

AAGTACGTCC

TACCCGCAAr-

GAGTCTACTG

AATGTrCGAG

GGAGTCGCGA

ACCTAC:GTAG

TTATACGTAG

GCTGATAGAT

GGAATATGAA

CTCCCTATTT

I'TTATGC

TAGCTC=IC

TTACAGGCGG

TGGGCGTGAT

TAGCAGGATA

ACGAGATCCT

GTATTCTGGT

CTTCG'ITAAT

TGTTATTTTT

CTAGTCAAAC

GCGTTrGGT

CCACCATGAT

ACCCGCGCTT

TATTTAATGT

ATGCCCCGAC

CTTTTGCAAT

TAAAGATGAA

TTGTTGCTCA

TAAAAGCTGT

AGCCTAAGAC

CAGAAAAGGA

TTTTAGCCAA

AAACGAACTC

GATCGCAAAC

TTGAAGTATC

TGACTCGTGA

TTACTA'TTT

GTT'I=AA

CCAAAAAGAA

ACGTCG TFI

GTGGAGCTTA

TGAAGTCGAA

AAAAATGTTC

TGTTTICCACA

TAC'I'ITCGCC

GACTAAAAAC

TGTAGCTCCG

TGAAGCCAAT

TAATAAT'GCG

AGGTCAGTCC

TGCTI'TCTG

TCAACTCATT

ATTTTAGCA

GGGTCTAAGT

TGATGCGAGG

AGCAAGCTTG

GGAGGAAGAC

TCGCACGAATG

GAACTTGGAG

ATTAGTGGTC

TATGGTGAGT

ACTGACTGCT

GACAAAACAA

AATGGGAATG

TAAACAGCCA

ACTGGCGCAA

TTGAATA.AG

192 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

EF097-2 (SEQ ID NO:370) MLTKNF LAGIVGGLLC CFGILVVAPA

VESASFWLYQ

LFLLETNPGP

IAGGMSGTLV

LKRQRGIEPV

PVTYQSVH-QM

SQEQSSLGSE

ETVFFTKETT

FSSWL IEANL

GVGVLVAFLL

FQIFNVCLSA

SMIKMflEEDQ

KWQPKTLWVI

STETNSTKQ I

YVVTRELAQA

LPLVHVFLEP

FGP VGQRKTA

PASPGSLVAI

VETVTPNYQQ

QAELKQLAQK

QKLVPLYAEN

YHLDLTQQNL

LKVLFFNNAI

GGATMIQLIG

LANAPTDARL

ILFVCDAGMG

YVPEKDMVSV

VRGSQTMOME

YVVTSFLNKK

NHGLLTPLGL

01 HE IYFFV

AVFSGIFVSF

SSAMGASILLS

QNFLEIKSYY

LLRQQAAKQG

EYQEWLEGGA

EGASQTGQS I

LMDPRLFLAV

LCSFAIASLL

RQLKAVNLEM

PQVLAKLTAS

VA IEVSKEPL

DRCF

EF097-3 (SEQ ID NO:37.) ACGAGG AATTGAACCA GTTTCAATGA TAAAGATGAA GGAGGAAGAC CAAGTrGGAAA

GGCTCAAGTG

ATGCCTGTGA

ATTCAAGCAG

GTTCAAAATT

TCTTCTCAAG

ATACAGAAGC

GAATTATTC

CTGGAAACAG

GCCTATCATT

AAAGAGTATC

CAGTCACACC

CCATGGGGGC

CTTACCAGTC

AATTGAAACA

TT'FrAGAAAT

AGCAATCTTC

TTGTTTTTTT

GCCAACAAC

TCTTTTTTAC

TAGATCTAAC

AAGAATOOCT

TAACTATCAG

TAGTTTGCTA

CGI'CATCAG

GTTAGCACAA

TAAATCCTAT

ACTI'GGTTCA

ATATGCCGAG

GGCGAAACAA

CAAGGAGACA

GCAACAAAAT

GGAAGGAGGA

CAAATTTTAT

AGCCGACAAT

ATGAAGT'GGC

AAGTACGTCC

TACCCGCAAG

GAGTCTACTG

AATGTTCGAG

GGAOTCGCGA

ACCTACGTAG

TTATACGTAG

GCTGATAGAT

TTTTIGTGA

TAAAAGCTT

AGCCTAAGAC

CAGAAAAGGA

TTTAGCCAA

AAACGAACTC

GATCGCAAAC

TTGAACTATC

TCACTCGTGA

TTACTAGTT'

GTTT

TGCAGGAATG

GAACTTGGAG

ATTAGTGGTC

TATGGTGAGT

ACTO.ACTGCT

GACAAAACAA

AATGGGAATG

TAAAOAOCCA

ACTOOCGCAA

TTTGAATAAG

EF097-4 (SEQ ID NO:372) RGIEPV SMIKMKlEEDQ VETVTPNYQQ ILFVCDAGMIG SSAMGASLLS RQLKAVNLEM

PVTYQSVHQM

SQEQSSLGSE

ETVFFTKETT

KWQPKTLVVI

STETNSTKQ I

YVVTRELAQA

QAELKQLAQK

QKLVFLYAEN

YHLDLTQQNL

YVPEKDMVSV

VRGSQTMGME

YVVTSFLNKK

QNFLE IKSYY

LLRQQAAIQG

EYQEWLEGGA

PQVLAKLTAS

VAIEVSKEPL

DRCF

EF098-1 (SEQ ID NO:373)

TAAATGAAAA

CTTGTACCAA

CAATTTCCAC

AAACCAATrA

CCGTTATCCA

AATATGACCT

GCCCAAACT

GTAAAACAAA

ACTTTAAGCA

GCTACGATTG

GAAGGTGCGG

ATITrCATTAG

ACTTGGACTT

AGACAAAAGT

TGAGTGCATI'

CAAATACGAA

CACCAGIGA

TTGACTATGC

ACTATGCAGA

TTGTTCAAGT

ATGGTCAA T

ACGGACGCGT

AATTAA.ACCC

GTACGTACTT

AAGTACCTGG

TGACAGATAC

AATGACATTG

AGCAGTCGAC

CCCAACGAAT

TCCAACTGAT

ATCTAGCTTA

AACACAAAAA

CTCAGATAAT

CAAAACTGAA

GGTTTCAGCT

AACTG.GGGCT

AATGAGCTGG

TTCAACCACA

ACCTGCTAAC

ATGGCAACCA

GGTGGTGAAT

CCAGTTGATC

CCGACAGGGC

TCTTI'TGGGG

TACAAAGATA

CGTGGGACTG

GCCAACCAAG

TCACAATCTG

GAATCAGTGG

GGCGATAGTG

AAATATGCGA

ACACGAAACT

CAACTI'TAGG

ACCAAACAAA

CGACGAATCC

CTAAGCCAGG

AACAA.ACGAT

ACGCTGGTGC

AGACAGGTTG

AACTAACAGC

CAAAGCCAAC

TCATGGCTGC

TAGATACCGC

AAAAATACAC

AA

CGCACTGGCA

CGGAGCGATT

AGACCCAGAT

GACAGCAGGT

TACCTCAAAA

CGACCAAGAA

GACGCTAAA-A

GGCCAAAGTA

GACAGCGCCA

TGGCGATAAA

TAAAACAAGT

GACAAC=TT

EF098-2 (SEQ ID NO:374) MKKTKVMTLM ATTTLGALAL VPMSALAVDG PITPVDPTDP TGPKPGTAGP LSIDYASSLS GEYQTNGAIQ FAPNTNPTNP VDPTNPDPDK FGEQTITSKN MTY-YAETQKY KDNAGADQEG 193 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

PNFVQVSDNR GTETGWTLKV KQNGQFKTEA NQELTAAXVT LSNGRVVSAS QSAKPTTAPA TIELNPTGAE SWVMAAGDKE GAGTYLMSWG DSVDTAKTSI SLEVPGSTTK YA.KKYTTTFT WTLTDTPANT GN EF098-3 (SEQ ID NO:375) AGTCGAC- GGTGGTGAAT ACCAAACAAA CGGAGCGATT

CAATTTGCAC

AAACCAATTA

CCGTTATCCA

AATATGACCT

GGCCCAAACT

GTAAAACAAA

ACTTTAAGCA

GCTACGATG

GAAGGTGCGG

ATTTCATTAG

ACTTGGACTT

CAAATACGAA

CACCAGTTGA

TTGACTATGC

ACTATGCAGA

TTOTTCAAGT

ATGGTCAATT

ACGGACGCGT

AATAAACCC

CTACGTACTIT

AAGTACCTGG

TGACAGATAC

CCCAACGAAT

TCCAACTGAT

ATCTAGCTTA

AACACAAAAA

CTCAGATAAT

CAAAACTCAA

GGTTTCAGCT

AACTGGGC-CT

AATGAGCTGG

TTCAACCACA

ACCTGCTAAC

CCAGTTGATC

CCCACAGGC

TCTTTT MGGG

TACAAAGATA

dGTGGCACTG

GCCAACCAAG

TCACA-ATCTG

GAAT-CAGTGG

GGCGATAGTG

AAATATGCGA

ACAGGAAACT

CGACGAATCC

CTAAGCCAGG

AACAAACGAT

ACGCTGGTGC

AGACAGGTTG

AACTAACAC

CAAAGCCAAC

TCATGGCTGC

TAGATACCGC

AAAAATACAC

AGACCCAGAT

GACAGCAGGT

TACCTCAAAA

CGACCAAGAA

GACGCTAAAA

GGCCAAAGTA

GACAGCGCCA

TGGCGATAAA

TAAAACAAGT

GACAACT'IT

EF098-4 (SEQ ID NO:376) VDC GEYQTNGAIQ FAPNTNPTNP VDPTNPDPDK PITPVDPTOP TGPKPGTAGP LSIDYASSLS FGEQTITSKN PNFVQVSDNR GTETGWTLKV KQNGQFKTEA NQELTAAI(VT TIELNPTCAE SVVMAAGDKE GAG EYLMSWG DSVDTAK<TSI WTLTDTPANT GN

MTYYAETQKY

LSNGRVVSAS

SLEVPGSTTI(

KDNAGADQEG

QSAKPTIAPA

YAKKYTTTFT

4 4 EF099-1 (SEQ ID NO:377) TGATGTTGTA GAGGGCTGAT ATGAAGAAAT TAGD-CAAGGT

TTATTTTTAG

ACACCACAGG

TTGCAGTTTG

AGAATTCAAA

TATGGAATTG

AAAACTCAAA

GATGGTTCTG

GCGGTAGATA

GTAAAAAGAA

GGAGGTTCAG

ACTATTrAATG

CTACAAATGA

AGTTCAkI'TG

GTCAITAATIT

GCAAGTGTTG

=TTGCTA

AGGAAATAG

CTGTATTTTC

AAAAAGTAGC

CTTCCGCTTG

CTGATTI'ATC

GGTTAGGACA

AAAAGGAATG

ATAGTGACT

TTTGAAGCT

AGGCTAGTGC

CCAATGTTGG

GTGGTCAATG

TGGGTACGGG

GTTGGACAGT

TTGCTCAAGG

AAGGTAAAAA

AGTAT='CG

GAAATGTTTA

TTAATTGTT~

TTCTAGTGAA

ATTAGAAGTT

GATTGGCAAT

GTTTAATTCA

ATGGGAITCA

GAAATCAGTA

ACTTAAAAGA

GTGGGAACGA

TAATAATTGG

TGGAGGAAAA

TTATGGC LTA GCATATG TI

CATAAAGAAT

TGGTGTGGCT

CAAGTTTACT

GACTTGGGGA

TCAGTCTTCT

AGTTGTTTTA

AGCGGAGATT

TCTAACTACG

ATGGAACAT-G

GCGATAGCTT

GGACGAAGAG

GCTTTACAAA

ATGTCTAAAT

GCTGGAACAA

TATAAACGAC

ATTGATGCCT

TCTGCTFri-T

GCGAGTQAAA

CCAAATTATT

ACTAGTATIT

ACTTATGAGC

TTAGATWC

TTTTATTGAA

TTTTTATTCT

CTTCCCAGTT

TGACGTCACA

AAAGTGGATL'

TTAATCCTTC

T'AATTTATT

TGGA=7TGC

CAAAAGATCT

AAGATGATCC

TTI'CTACAGG

TGGAAAAAGT

TTG~TGAA-AA

TTGGTAATGA

CAGATATTAA

ATGGGCATAC

AAAACGCTGA

CACAMMTAC

AGGAGAGATC

TCCTTTTFIA

TCAGCCCGCT

TGGCGGAACG

AAATCCTGCT

GTTAGCCT

AAATTITGCA

GTGGAATAAG

GAATACAC I'1

CGCAGAACAA

TTCCATGGGC

GATGGGGCAA

ACAAGAGGT

T1'ATCCTTGG

AGCAGGAGAT

TCGTGTAGTG

ACAAGGTCAA

CAGCATAGTA

a EF099-2 (SEQ ID 1NO:378) frflCLS VFFLLKGEIM KKLGKVLIVS CFIFILPFLL FLGVFSSSES GDSSQFQPAT PQEKVALEVS NYVTSHGGTL QFASAWIGNN EHESGLNIPAR IQSDLSFNSA IAFNPSLGGY GIGLGQWDSG RR\TNLLNFAX SQKKEWKSVA LQMDFAWNXD GSDSDLLKRM SKSKDVNTLA 194 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

VDILKLWERA GTKDDPAEQV KRKASANNZWY KRLSTGSMG INGGQCYGLS AFFVEKQGGL QMMGTGHMFA SEIGNDYPWS I NFGQGGVAT S IYGHTGVWA SVEGKNKFTT YEQNAEQGO I

K

EF099-3 (SEQ ID NO:379) TAGTGAA. AGCGGAGATT CTTCCCAGTT TCAGCCCGCT GSANVGGGK I CALEKVMGQT SIGWTVIKNP NYSDIKACDV VAKYFRTWGL CFPHVTS IVR

ACACCACAGG

TTG-CAGT='

AGAATTCAA.A

TATGGAA=~

AAAAGTCAAA

GATGGTTCTG

GCGGTAGATA

GTAAAAAGAA

GGAGGTTCAG

ACTATTAATG

CTACAAATGA

AGTTCAATTG

GTCATTAATT

GCAAGTGTTG

ATTGTTGCTA

AGGAAAT

AAAAAGTAGC ATTAGAAGT

CITCCGCTTG

GTGATTTATC

GGTTAGGACA

AAAAGGAATG

ATAGTGACTT

ITTGAAGCT

AGGCTAGTGC

CCAATGTTGG

GTGGTCAATG

TGGGTACGGG

CTTGGACAGT

TTGGTCAAGG

AAGGTAAAAA

ACTATTTICG

GA'ITGGCAAT

G'1TTAATTCA

ATGGGATTCA

GAAATCAGTA

ACTTAAAAGA

GTGGGAACGA

TAATAATTGO

TGGAGGAAAA

TATGGCTT1A

GCATATGT=

CATAAAGAAT

TGGTGGCT

CAAGTTTACT

GACTTGGGGA

TCT.ACTACG

ATGGAACATG

GCCATACCTT

GG.;CGAAGAG

GCTTTACA;A

ATrTCTAAAT

GCTGGAACAA

TATAAACGAC

ATTGATGCCT

TCTGCTIT

GCGAGTGAAA

CCAAA'ITATT

ACTAGTAT

ACTTATGAGC

TTAGA'I IC

TGACGTCACA

AA.AGTGGATT

TTAATCCTTC

TTAKI'TTATT

TGGATTTTGC

CAAAAGATGT

AAGATGATCC

TTTCTACAGG

TGGAAAAAGT

T'rGTTGAAAA

ITGGTAATGA

CAGATATTAA

ATGGGCATAC

AAAACGCTGA

CACATGTGAC

TGGCGGAACG

AAATCCTGCT

GTTAGGCGGT

AA.ATTTI'GCA

CTGGAATAAG

GAATACACTI'

CGCAGAACAA

'PrCCATGGGC

GATGGGGCAA

ACAAGGAGGT

TTATCCTTGG

AGCAGGAGAT

TGGTGTAGTrC

ACAAGGTCAA

CAGCATAGTA

EF099-4 (SEQ ID NO:380) SES GDSSQFQPAT PQEKVALEVS NYVTSHGGTL GIGLGQWDSG RRVNLLNFAK VDI LKLWERA GTKDDPAEQV INGGQCYGLS AFFVEKQGGL INFGQGGVAT S IYGHTOWVA

QFASAWIGNM

SQKKEWKSVA

KRKASAhNY

QMMGTGHMFA

SVEGKNKFTT

EHESGLNPAR

LQMDFAWNKD

KRLSTGSMGG

SEIGNDYPWS

YEQNAEQGQI

IQSDLSFNSA

GSDS:DLLKRM

GSANVGGGKI

SIGWqIVIKNP

VAKYF'RTIWGL

IAFNPSLGGY

SKSKDVNTL.A

DALEKVMGQT

NYSDIKAGDV

DFPHVTS IVR EF100-l (SEQ ID NO:381) Pt..

a

TAN'ITATGGC

ACATI'ATTAG

CCTAACTTAG

ATrGTAGAAT

GAATTAGTCA

CAATGA

AATATGGAAG

AAATGTTGAT

CGAAACATAA

CACAAATCGA

ACGAAGGCTA

GAGTTTATA

TGTCTTATTG

AGAAACAGTT

GCTCTACACA

CATTACTAAA

ATGAAAAAGA

ATTA7TCCG

GATAAAAAAG

CTAGAAAAAA

GAGCAGTTAC;

AACAAAAATA

TArI'ATT

GCAAT'-A-AGC

ATAAGACGCCC

ATAALATATAC

CGCAGGGTT]?

ACTTTTTGTC

AATCGTAAAA

TTCCTTAAAT

AGCAGAAAAG

EFlOO-2 (SEQ ID NO:382) MKKKQKYAGF TLLEMLIVLL I ISVLILLFV PNLAXHKE'IV DKXGNEAIVX IVESQIELYT LEKNKTPSLN ELVNEGYITK EQLDKYTAEK Q EF100-3 (SEQ ID NO:383) TAA AGAAACAGT GATAAAAAAG GCAATGAAGC AATCGTAAAA ATTGTAGAAT CACAAATCGA GCTCTACACA CTAGAAAAAA ATAALGACCCC TTCCTTAAAT GAATTAGTCA ACGAAGGCTA CATTACTAA.A GAGCAGTTAG ATAAATATAC AGCAGAAAAG 195 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

CAAT

EF100-4 (SEQ ID NO:384) KETV DKKGNEAIVK IVESQIELYT LEKNXTPSLN ELVNEGYITK EQLDKYTAEK Q EF100-1 (SEQ ID NO:385)

TANTTATGC

ACA'FIATTAG

CCTAACTTAG

ATTGTAGAAT

GAATTAGTCA

CAATGA

AATATGGAAG

AAATGTTGAT

CGAAACATAA

CACAAATCGA

ACGAAGGCTA

GAGFFI'TATA

TGTCTTATTG

AGAAACAGTT

GCTCTACACA

CATTACTAAA

ATCAAAAAGA

ATI'ATICCG

GATA.AAAAAG

CTAGAAAAAA

GAGCAGTTAG

AACAAAAATA

TATTGATTTT

GCAATGAAGC

ATAAGACGCC

ATAAAtATAC CGCAGGG TTT

ACTTTTTCTC

AATCGTAAAA

TTCCTTAAAT

AGCAGAAAAG

EFI00-2 (SEQ ID NO:386) MXKKQKYAGF TLLEMLIVLL IISVLILLFV PNLAKHKETV DKKGNEAIVK I'JESQIELYT LEKNKTPSLN ELVNEGYITK EQLDKY'IAEK Q EF100-3 (SEQ ID NO:387) TAA AGAAACAGTT GATAAAAAAG GCAATGAAGC AATCGTAAAA ATTGTAGAAT CACAAATCGA GCTCTACACA CTAGAAAAAA ATAAGACGCC TTCCT~rAAAT GAATTAGTCA ACGAACGCTA CATTACTAAA CAGCAGTTAG ATAAATATAC AGCAGAAAAG

CAAT

EFIO0-4 (SEQ ID NO:388) 4.

KETV DKXGINEAIVK IVESQIELYT LEKNKTPSLN ELVNEGYITK EQLDKYTAEK Q EF101-1 (SEQ ID NO:389) 44

TGAGGAGATG

GTAATTGCGG

GCTGTCAAC

CAACAAATTT

CAAAAAATAA

ACAGAATTAT

AGCTTACCCA

GATAAA ETTA

CAATATCAAA

GCGGATTTAG

ATTCCAGAAG

ITAGCTGGAA

CTAACTTCTG

CCTCCTGAAG

AG'ITATAGCG

CAAGCAACCA

AAAGAGGGTG

GTCCAAGTCG

GACCCAGTGG

AAACGAAGAA

TrGGGGGCAT

AAGCGCCTAA

ATA'ITAACGG

CAAAGGATCC

TTACTTATGA

AATTAGAAAC

ATAAAACTAA

CAGAAGTCGA

GArGCGAAGCA

TAAAAGATGC

AAGTGAATGA

TTI'CCAACAA

GCAACAGCGA

TCAAAATTGC

TrGATTTAGG

AACAGG-CCTA

GGCAAGAAAA

TTATTCTTC

AATGAAGAAG

CGTAACTGTG

AGATGACTGG

TGTCATCCAA

AGAGATI'AAG

AGATGAGGCG

GAAGCGGGCG

AGAAGAAGAC

TGCAGTAGAT

ATATMT=CC

CAATTICACCG

AAAGGACTTG

TGTGGITG T(

TGCCGCGAGT

GTTGGCCAAT

CGATTTAGGG

CGTTTTAGTG

TGGCGACAAA

AAAAAAACCA

A-AAACGATAA

AATGCG'TAA

GGAATTGACT

CCGGAACAAA

GTGAAAAACG

GTCACAAAAG

AATATCTATA

CGCACGATCT

GAAGAGATTA

ACAAAGGCTA

AlITI'TACGGT

ACTAAAATTA

GATGGCTCAA

GGCAATCCAG

TTAGACAAAG

GCGATTGAGT

AATGA7=TG

ATGGCGATTG

GTAAAAGTCG

TTATATTCGG GGCAGTTGCG ATAAAIAATGC ACAACAAGTA ATI'TTGACGT TCCCGACTTG TGGAAGCCTT TGCGCGTGAT GCGATGTCGT AGATGCAGC AAATTGAGGC ACAACAAAAT ATAAGTGGAA TCGGGCCATT CTGGTGATGA TTTAAATG-AA CCTTCACCAA TGAAACCTTA ATTTCAAAGG TCGTGTATCA TAACTTCAGA AGATCTTTAT GTGTTGGGCA AAAAGCTAAA TTTCTrACAT CGATGATAAT AGGGCGGCAC AACGATGTCT TCAMAAATGG CTACCATATG TACCGAAAAA AGCGATTCAA GAACCATCAT TCGTCGTGAT AATClrGCTT AGAATCACCC GTGATATI'GT TGAATCAGAT 196 TABLE 1. Nucleotidc and Amino Acid Seqeuences of Efaecalis Genes.

GCAGCGATTG CI'CTGATGA ATCACCAACC AACGAATCAA TGACAGATGC GTCGAAATAG EF101-2 (SEQ ID NQ:390) MKKK TIIILGAVAV IAVGGIVTVN ALNKNAQQVA VKQAPKDJWG IDYFI)VPDLQ

QIYINGVIQP

LAKLETKRAN

DLGAKQYIST

TSVSNNVVVD

ATIDLGDLGA

RVVISSKKPV

EQMEAFARDQ

IYN'KWNRAID

KANFI<GRVS I

GSISYIDDNP

IELPKKAIQK

KVGDIVESDA

KITKDPEIXV

KFNKTKEEDR

PEVKDANSI

PEGNSDAASG

EGEQAYVLVN

AIASDESATN

KN'GDWVDAGT

TMSCDDLNJEQ

LRLTSEDLYL

NPEGGTTMSS

DFGTIIRRDV

ESMTDASK

ELFTYEDEAV

YQTEVDAVDE

AGKVNE-KDLT

YSVKIALANL

QVGQENGDKM

TK:-IEAQQNS

EITFTNETLA

KISVGQKAKL

DKVKNGYHMQ

AIESGLESAD

EF101-3 (SEQ ID N0K391) TAAAAATGC ACAACAAGTA GCTGTCAAGC AAGCGCCTAA CAACAAATTT ATATTAACCG CAAAAAATAA CAAAGGATCC ACAGAATTAT TTACTTATGA AGCTI'AGCCA AATTAGAAAC GATAAATrTA ATAAAACTAA CA.ATATCAA.A CAGAAGTCGA GC'CGAT ITAG GAGCGAAGCA ATTCCAGAAG TAAAAGATGC TTAGCTGGAA AAGTGAATGA CTAACTTCTG TITCCAACAA **CCTCCTGAAG GCAACAGCGA AGTTATAGCG TCAAAATTGC CA.AGCAACCA TTGATTTAGG *.AAAGAGGGTG AACAGGCCTA *GTCCAAGTCG GGCAAGAAAA GACCGAGTGG TTATrTCTTC GCAGCGATTG CTTCTGATGA

AGATGACTGG

TGTCATCCAA

AGAGATTAAG

AGATGAGGCG

GAAGCGGGCG

AGAAGAAGAC

TGCAGTAGAT

ATATATTTCC

CAATTICACCG

AAAGGACTTG

TGTGGT1'GTG

TGCCGCGAGT

GTTGGCCAAT

CGATTAGGG

CGTTTTAGTG

TaCC;ACAAA GGAA7DGACT

CCGGAACAAA

GTGAAAAACG

CTCACAAAAG

AATATCTATA

CGCACGATGT

GAAGAGATTA

ACAAAGGCTA

ATTTACGGT

ACTAAAATTA

GATGGCTCAA

GGCAATCCAG

TTAGACAAAG

GCGA'ITGAGT

AATGATTTTG

ATGGCGATTG

ATTTTGACGT

TGGAAGCCTT

GCGATGTCGT

AAA=TAGGC

ATAAGTCGAA

CTGGTGATGA

CCTTCACCAA

A=TCAAAGG

TAACTTCAGA

GTGTTGGGCA

TT-rCTTACAT

AGGGCGGCAC

TCAAAAATGG

TACCGAAAAA

GAACCATCAT

AATCTGGCTT

GTGATATTGT

TGACAGATC

TCCCGACTTG

TGCCCCTGAT

AGATGCAGGC

ACAACAAAAT

TCCCCATT

TTTAAATGAA

TGAAACCTTA

TCGTGTATCA

ACATCTTTAT

AAAAGCTAAA

CGATGATAAT

AACGATGTCT

CTACCATATG

AGC-GATTCAA

TCGTCGTGAT

ACAATCAGCC

TGAATCAGAT

GTCGAAAT

AAAAAAACCA GTAAAAGTCG ATCAGCAACC AACCAATCAA EF101-4 (SEQ ID N~O:392) KNAQQVA VKQAPKDDWG IDYFDVPDLQ

S

a..

C

a

QIYINGVIQP

LAKLETKRAN

DLGAKQYIST

TSVSNNVVVD

ATI DLGDLGA

RVVISSKKPV

EQMEAFARDQ

IYNKWNRAID

KANFKGRVS I

GSISYIDDNP

IELPKKAIQK

KVGDIVESDA

KITKDPEIKV

KFNKTKEEDR

PEVKDANSP I

PEGNSDAASG

EGEQAYVLVN

AIASDESATN

EF102-1 (SEQ ID NO:393)

TAAACATITG

ATTAAT'ATA

TTAAGTCAAA

CACCCTCA-AG

TTTAGCGATG

GCAAAACAAA

GGTITATCAGA

TGGGAAAAGG

GAAGTGAACG

AGACATTCAG

TGAAAAAAAC

CAA ['rGG

AGGTAACAAT

GCAGCACGCT

CAGTATTTTG

AAAACCCTT

CTGGAACAGA

GTTATAAACT

AGGTGAATGT

AACA=IAAA

AACGATTGGT

TCATTATGAC

CTCAGAACGT

TATTGAACCA

GCCATCAATG

TATIGATACA

CCATTCCATA

KNGDVVDAGT

TMSCDDLNEQ

LRLTSEDLYL

NPEGGTTMSS

DFGTIIRRDV

ESMTDASK

CTCTTrTTA AAT'rGGTCGT

CCTACGATTG

CTAAGTAAAC

ACTACGTCAT

GGTGTTAGTA

TCTGATAAAC

AATATGGTTM

AAAAGATTAG

ELFTYEDE-AV

YQTEVDAVDE

AGKVNEKDLT

YSVKIALANL

QVGQENGDKN

TTACTCAAAA

TAITI'CGAC

CTITTGCCGA

TGTATGAAGT

TATATGCAGA

TI'CCAACAGA

CGAAACTAGT

CACAAAAGAT

GTGGTGC

ACGAAAGGGG

TTTGGCTCTA

TGAAATTACT

TGACGGAACT

ATACAATGGT

AGTGACGCAC

ATCGGTTCTT

GATTTGGGAA

AGTTGATATA

TKEIEAQQNS

EITFTNETLA

KISVGQKAKL

DKVKNGYHMQ

AIESGLESAD

TABLE 1. Nucieotide and Amino Acid Seqeuences of E. faecalis Genes.

AAATCTATTG

CATAATACCA

?I'AAAmTAT ATTr.GGAAT'C

AAAAAATCAG

ATGGCTGGTG

GGTTCTTrAA

TTCCATTTAG

ATTTCTATTT

CCAGATCCT

ATTTCCATGA

GTAGA.AACAG

ATTCGTAAAG

CGTGCGGAAA

GAAACTAAAT

TATCCCAATC

ACTGGGGAAA

GCTGAGTTTA

TGGAGTGAAG

ACTTTGGCTT

TTCTGGCAAG

TCCATCAAAA

AAAGAACA-AG

ACTGCCGAAA

GAAATCACAG

GGCTATCGTA

CCAGAAGGAT

GACGACTATG

ATTAAGATGG

AACCGTTTGA

TGGAAAGACG

AGATATAACT

GAAGCCACAA

ACAACCGCAA

ACCGCTGAAC

GTAGCCTTTG

CAAACCGTCA

GATGGTTCGC

ACCCATGATG

CCAGATGGTA

AAAGAATTTA

ACTAAGTTTA

AATGAAGATT

AGTACGCCAA

ACAGTGAAGA

WI'ATCTI'GA

AAGGAAAAAT

CTGTAAAAAC

CTGACT=GA

AATTAGTCCT

CTGGTACTGG

CGCTTGATAA

AGATCAAAAA

ATT=GGGAA

TGGATGGAAT

ATATCATTGA

CTTCGAAAA-A

GTACTGATCT

ACAGCCCAC

CACCAAAAGA

CTAGTAATGG

AAACCGTGGC

CCAC'ITGAC

AAGGAGCTGA

C'ITTTAAAAC

TAGATGAAAA

AAACCAAAGC

AAGTTGATAA

TTATTCGCTT

CTGGATTTAA

GTGCTGAAGA

AG'T~TGAAAA

TTCAAAAGAT

CGAAGAGTGA

TGACCOTTCC

TGCTTTATGA

GAAATAAAAA

TGCATGAAAT

AAGCTGCCCA

CGTTGGCGAA

AAGTTTTGGA

AAGCAGGCAA

ATTGTACGAT

AAACTTFAC

TACTGGATGC

CGAACAAAGA

CCAAAACCGT

CTTTTACGGA

TGAAAGAAAA

A.ACAACCGGA

CATTCCCGCA

TTTTTCGAC

V'AATAAGGCA I

.ATTITAGGT

rAAAGTCGTC

EACTCCAAAC

AACTCCACTC

GCCCAATACC

.ATCGATAAA

AGCTTTACCT

TCCCCATGGT

TACCACACCC

TATGCGACAA

I'TGGAATGAC

TGGTGAAATT

GCTTGCTAAT

I=CGTGAAT

TCI'GTTACC

AAAAGAAGAC

ATATACTCTC

AGAATTAGTG

GAACCAACTT

ACCTGAAGGA

TAACGAAAAA

TGGCCITIAT

CGACTTATCT

TAAAGCGACC

TCTTCC ITAT

TACACCACTA

GTATGTCTTT

TTACCAGAAA

'TTTGCCCGAG

ATTGAATACC

CAAAGAACAC

AGAAAAAGAC

CAAAGAGAAA

TAAAAGCATC

TGAGCCAGTA

TGAACGCCAT

TCATGGTCAC

CTCAAAACAA

AAT=TGAAA

AC ITGCGGAA

AATCAATTAC

ATCTCAAACC

AACACCAGCT

AACTGGGGAG

TGCTGGGTAT

.TTGAGGAGT2

AATCGACAA

~AAAATACGG

ECTAATTCCA2 ;CTTATAAAA2

PACGCTATTA

;AATCACGTG

TCAAAAGATG

A.CAAAGGTAA

ATCGCTGaCCA

AIAAGGTCAAA

~ATATrCTC

GTCCAAGAAA

GCTTTGGAAC

ACCTTCAAAC

AGTA.ACGTAA

AAAGATACCG

TTACTGCAA

AAGGGAACGA

GCCGTTAAAC

TATACTMG

AATGCCGTAA

TTCTTTAAAT

TTAAAGTG-T

ACAGCTTGTA

GGGATTATT

GAAATCCGTT

ACCATTACC 6

CTAACTAACA

AAAGAAGATA

CTTGA=rTA

TGGTATGTCG

GAAAAAGCCA

ACTGGAACTT

GTCTTGTTCA

GCGAAGGATG

GTTTCCATCC

GTGATGGATA

GCTTTCGAAA

TCTGGCAAAA

AAAGTAGATA

GAAAAAGATG

TTA.ACACCAA

GTTCCAAGTA

.AAAAATCCA

TATTTCTGGA

TCAAAA.AAA I

TTTAATAGA

:GAATATAGA

kATCAGGAAC2 kAGCAGGACT kAATTAATGTC kTATTGTACC rGACAACAGA

CTATTACTGA

CCATTAAAGC

TTCTTTTAGA

I'AGCTGGAAA

'A.ACAACGGA

TGGGA CCTA

CAACAAAAGT

AAGGGCAAAA

GTAATGAGAG

AAGATGGTCA

AAGCTTCTGA

ACCTAGCAAT

ATGAA.ACGAA

TTACTCGAGA

TTGCTGGATC

CGCCATTGGA

ACGAGCAATT

TACTTGAAGA

CTACATTTAA

AAGAAGGACA

ACGAGTFII'C

GTTTGACTI'C

CCGAGCTAGT

TAGCTCAAGC

AACCOGTGGT

GGAAAATTCT

ATTATGTGTA

CTAGCTTGAA

AAACAAAAC

TG'FIGATGA

CAATTCTGTA

TTCACCATGA

CC:GGAAAGTA

GAAACGTGAA

AAGAAGTGCC

ATTCTCAAGA

ACGTTCTACT

ATCGCCGCAA

CCAAGTTTT

CAAAAATGAA

ETACCGTGTA

kTTAA.CATTG

LCAAACTGTG

GGAAACTAAG

AGAAACGGTT

TA.AAGATGGG

~AAATCGGTG

GGGCGAGACC

GAAGACTGGG

TACAT?1GCC

TGAAAAAGGT

TTACGTGACA

CGAGTTAAAA

CCAAGAAATTI

TCAAGGGAAA

AGCTG'TAAA

TGAAACAGTG

TAACGAGTAT

GTATCCTGTA

TGTTACGGCA

GGCTGATGGC

AGGGACCAAN

AGGTTTTGAT

AATAGAGGCT

GGAAAACAAA

AAAACAACCA

TGTTAGTCTG

TCTTGCGACT

TGATAALATTG

CATTGATGTG

GATTGCCGAA

CCATAAATA

TGAA.AACAAG

CAATCAAGCA

CCACCTAGAA

TGTGTCGGT'

TGCTTTACTA

AGTGAATCAT

TCCAGAAGGA

TGGAAAACAC

AACCATACCG

ATCTAGTCCC

GTTAG~TTGGC

TTAA

EF102-2 (SEQ ID NO:394) MKKTTFKN WSLFATLALL SQTIGGTIGP TIAFADEITH PQEVTIHfYDV SKLYEVDGTF SDGSTLSERT TSLYAEYNGA YQKNPLPSMS DKAKLVSVLW EKACTDIDTN MVAQKI4IWEE SIEGKINKAI EEYQKKPSFH NTTVKTILGQ STTLIDKNEL GNQLVLTPNS NSKSGTLTLK KSAGTGTPVA YKKAGLQTVM

KQTVFCIEPG

VNGYKLHSIX

NLSEFDKVVQ

AGALOKPNTY

VS IPTEVTHG

RLGGASVDIK

NTAN IDYRV I

AIKINVETKG

TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faeca fis Genes.

SLKIKXI DKE

DPYMIDTTPM

RKDSPAGEIV

ANQTVALVTS

SEAFKTELVI(

IKKVD)NNEKN

ITGAEDKAT

DYAKSEYVFT

KDGNKKLNTL

TATLANKEKT

TVNCTIERHV

DGTNKEIWKS

EOLKEKSQTL

IL IFSTAGYY SGD IVPETVF

AATIKAGETI

QEITTDEKGR

NVKGQNQEIT

GTKASDETVT

AVITRDVTAK

ACNEQLGFDG

ITEEGQKQPI

DFTELVDKLR

GTWKILRKLT

SIQTKAHLED

GKIEHEVNDK

TPKEVPTI PS

FWNRRN

HLDFGKAL.PS

SMTSKNMRQK

AETPKELANA

GETTLTKEDK

LALDEKNQVA

EQVIRFGFDF

YGKFENLPYG

KMVTVPYEKL

YNLHEIKEDW

AEQVLDKS IV

GSQTFTHGDV

EFTKTVLAEK

TPKQPETPAV

KDVTTDKDG I

GQILLEKTGV

LE-LGTYVVTE

DTGNESQGKA

VK}(LAINEYF

FKFAGSADGT

DYLLEEIEAP

TNNEFSVSLN

YVVAQAIDVE

LFN.YVYENXV

MOMFDD VS VT

VDTGKYPEGT

PSNSQESSPT

SILDGIPHGT

ETGTDLWNDN

TKSSNGFVNT

EFKGAEYTLF

WQETKAPEGY

AETGFNDLSF

EGFQKITPLE

RLMLYOLPEK

ATKAAQEKDE

AFEAGNEPVA

HD-VLJJGSKEA

KFTFTE INYE

VKTFPQTEY(

KVTITEXSVP

YSLAGNTFAI

FKPTKVELKY

TAXKDGQAVKW

TLDETKYPVS

KVS PLEGTXE

IRSTFKENXD

EDSLTSLATW

KAKPVVIAET

KDASLNNQAQ

FETILYALLP

KDGNVNGKHN

NSNVLLLVGF

EF102-3 (SEQ ID NO:395) TT TAGATGAAAA GAACCAAGIT GCCGTTAAAC ACCTAGCAAT TAACGAGTAT a.

a.

a..

a a.

a a a a a a a a

TTCTGGCAAG

TCCATCAAAA

AAAGAACAAG

ACTGCCGAAA

GAAATCACAG

GGCTATGGTAL

CCAGAAGGAT

GACGACTATG

ATTAAGATGG

AACCGTTTGA

TGGAAAGACG

AGATATAACT

GAAGCCACAA

ACAACCGCA.A

ACCGCTGAAC

GTAGCCTTTG

CAAACCGTCA

CATGGTTCC

ACCCATGATG

CCAGATGGTA

AAAGAAFI'TA

ACTAAGTTTA

AATGAAGATT

AGTACGCCAA

ACAGTGAAGA

AAACCAAAC

AAGTTGATAA

TTATTCGCTT

CTGGA'ITTAA

GTGCTGAAGA

AGTTTGAAAA

TTCAAAAGAT

CGAAGAGTGA

TGACCGTTCC

TGCTTTATGA

GAAATAAAAA

TGCATGAAAT

AAGCTGCCCA

CGTTGGCGAA

AAGTTTTGGA

AAGCAGGCAA

AITGTACGAT

AAACTTTTAC

TACTGGATGG

CGAACAAAGA

CCAAAACCCT

C T=ACGGA

TGAAAGAAAA

AACAACCGGA

ACCTGAAGGA

TAACGAAAAA

TGGCTTTGAT

CGACTTATCT

TAAAGCGACC

TCTTCCTTAT

TACACCACTA

GTATGTCTIT

TTACGAGAAA

TTTGCCCGAG

ATTGAATACC

CAAAGAAGAC

AGAAAAAGAC

CAAAGCAAA

TAAAAGCATC

TGAGCCAGTA

TGAACGCCAT

TCATCGTGAC

CTCAAAAGAA

AA'ITTGGAAA

ACTTGCGGAA

AATCAATTAC

ATCTCAAACC

AACACCACCT

TATAC=TTGG

AATGCCGTAA

TTCTTTAAAT

TITTAAAGTGT

ACAGCTMTA

GGGGATTATJT

GAAATCCGTI'

ACCATTACCG

CTAACTAACA

AAAGAAGATA

CTTGA=ITTA

TGGTATGTCG

GAAAAAGCCA

ACTGGAACII'

GTCTTGTTCA

GCGAAGGATG

GTTTCCATCC

GTGATGGATA

GCTTTCGAAA

TCTGGCAAAA

AAAGTAGATA

GAAAAAGATG

TTAACACCAA

GTTCCAAGTA

ATGA-AACGAA

TTACTCGAC;A

TTCMTGATC

CGCCATTG'-A

ACGACCAATT

TACTM'AAGA

CTACATTTAA

AAGAAGGACA

ACGAGTTIC

GTTI'ACT'C

CCGAGCTAGT

TAGCTCAAGC

AACCGGTGGT

.GGAAAATTCT

ATTATCTGTA

CTAGCTTGAA

AAACAAAAGC

TGTTTGATGA

CAATTCTGTA

TTGAGCATCA

CCGGAAAGTA

GAAACGMAA

AAGAAGTGCC

ATI'CTCAAGA

GTATCCTGTA

TGTTACGGCA

GGCTGATGGC

AGGGACCAAN

AGGTTTTGAT

AATAGAGGCT

GGAAAACAAA

AAAACAACCA

TGTTAGTCTG

TCTTGCGACT

TCATAAATTG

CATTGATGTG

GATTGCCGAA

GCATAAATTA

TGAAAACAAG

CAATCAAGCA

CCACCTAGAA

TGTGTCGGTT

TGCTTTACTA

AGTGAATGAT

TCCAGAAGGA

TGGAAAACAC

AACCATACCG

ATCTAGTCCC

EF102-4 (SEQ ID NO:396) LDEKNQVA VXHLAINEYF WQETKAPEGY TLDETKYPVS

IKKVDNNEKN

ITGAEDKATT

DYAKSEYVFT

KDGNKKLNTL

TATLANKEKT

TVNCTIERHV

DGTNKEIWKS

AVITRDVTAK

ACNEQLGFDG

ITEEGQKQP I

DFTELVDKLR

GTWKILHKLT

SIQTKAHLED

GKIEHEVNDK

EQVIRFGFDF

YGKFENLPYG

KMVTVPYEKL

YNLHEIKEDW

AEQVLDKS IV

GSQTFTHGDV

EFTKTVLAEK

FKFAGSADGT

DYLLEEIEAP

TNNEFSVSLN

YVVAQAIDVE

LFNYVYENKV

MDMFDDVSVT

VDTGKYPEGT

AETGFNDLSF

EGFQKITPLE

RLMLYDLPEK

ATKAAQEKDE

AFE.AGNEPVA

HDVLDGSKEA

KFTTE INY

KVSPLEGTXE

IRSTFKEN!(D

EDSLTSLATW

KAKPVVIAET

KDASLNNQAQ

FETILYALLP

KDGNVNGKHN

199 TABLE 1. Nucleotide and Amino Acid Seqeucnces of E.faecalis Genes.

EDLKEKSQTL TPKEVPTIPS TPKQPETPAV PSNSQES8PT VX EF103-1 (SEQ ID NO:397)

TAAGATAGGT

ATTACTTTAG

CATCATGCAC

CAAACAAGAG

GTTTATGATG

AAATATGATG

GGTGACGAAG

ACACAAAATT

AAGCGTI'TAG

TATCATGGGA

AAAATI'GTGA

AAAGTCGTAG

GCGAAATTG

CGCGGCGACT

ATTGGTACGA

ACGTATACTC

GAACCTI'ACC

TTATCAAAGA

TAACATTAAG

CCAATCCAAA

GCGACAAGGC

CTGCTGGTAA

GTGAAACCGG

GAACATTT'I

ATCAAGCCGT

GGAAAGATAA

AAA-AATTAGC

CAAATAAATC

ATAAAAACGG

ATCCAAACAC

TTGGGTACTT

ATCGTTACGG

GAATGGGTAA

AAGGCACATA

AAAGGAGCCA

TACGTI'ACTT

TACTGCTACA

GACTAAGAT

TGAT'ITAACG

ITTTTACGAG

TGTGACAGCT

AGTGGATTTA

ACTGGGGAAT

1TMACAAAT AGGG3-ATAAA

TAATGATITG

AAGTAAATAT

CCAAGTGGTG

CGCGGCGCTA

AGATAATCCT

TCATCCAGCC

TCGCTTTATCA A.AAAGAAAG-T ATAGCAGGTT ATGCAAGTCC GCAAAT1rrAG GCAAACATCA -TATCTGGCA CGGACTGGCA GCAGAAAATG CTAATTTTAT TITTTCGACA AAAATACTGG CATGGCACAA AACGAATTTT ACCGAAGTGA CTAAAGACNA GATGTTGAAG TTrACGTGGA GGACGTG;AAG CATTAACCAA ATrTTAGGAA CAACCTTGTG ACAGCGGCCA ATCAAAATT GAATTTTCA ATTTACAAAC GACAATAACA AGATTZCGGC GAATTAACGG AACTAAACAA GGTAATGATA 'ITCAAGTGTT TTTACTT-TCT AA

ATTAAGTTCG

AGCATrrGCA

AAACAATGGC

AGGAACCCGT

TGGTTTAGCA

GGAAACCCGT

AATTTCGCGG

ATTACTTAC

ACACATCCCT

TCAAACTGGC

GAATGGCACA

CATTAGT=A

AGGTGAAACC

CCATGTATCT

TGATCGA'IT

CGTGGAACAT

EF103-2 (SEQ ID NO:398) a.

a a a MKKKVLSSI TLVTLSTLLI AGYASPAFAD HAANPNSATA NLGKHQNNGQ TRCDKATKIL SGTDWQGTRV YDAAGNDLTA ENANFIGLAK YD)GETGFYEF DEGTFFVTGD GTKRIL.ISRT QNYQAVVDLT EVSKDXFTYK RLGKDKLGND HGKKLAFTNG REALTNQTGK IVTNKSGDK I LGTTLWNGT.K VVDKNG;NDVT KFDPNTSKYE FFNLQTGETR GDFGYFQWVD NNKIRAHVSI GTNRYGAALE YTRMGKDNAG NDIQVFVEHE PYQGTYHPAF TF

FDKNTGETRG

VEVYVE14I PY AANQNFI SLA

LTELNNDRFT

EF103-3 (SEQ ID NO:399) TCATGCAG CCAATCCAA-A TAGTGCTACA GCAAT~AG GCAAACATCA AAACAATGGC

CAAACAAGAG

GTTTATGATG

AAATATGATG

GGTGACGAAG

ACACAAAATT

AAGCGTTTAG

TATCATGGGA

AAAATTGTGA

AAAGTCGTAG

GCGA.AATITG

CGCGGCGACT

A'ITGGTACGA

ACGTATACTC

GAACCTTACC

GCGACAAGGC

CTCCTGGTAA

GTGAAACCGG

GAACATTTTT

ATCAAGCCGT

GGAAAGATAA

AAAAAT1'AGC

CAAATAAATC

ATAAAAACGG

ATCCAAACAC

TTGGG'ACTT

ATCGTTACGG

GAATGGGTAA

AAGGCACATA

GACTAACATT

TGA=TAACG

T'I=rACGAG

TGTGACAGGT

AGTGGATTTA

ACTGGGGAAT

TTI'TACAAAT

AGGGATAAA

TAATGATGTG

AAGTAAATAt

CCAAGTGGTG

CGCGGCGCTA

AGATAATGCT

TCATCCAGCC

TTATCTGGCA

GCAGAAAATG

TITCGACA

GATGGCACAA

ACCGAAGTGA

GATGTrGAAG

GGACGTGA.AG

A'ITTTAGGAA

ACAGCCGCCA

GAATTI'TCA

GACAATAACA

GAATTAACGG

GGTAATGATA

T

CGGACTGGCA

CTAA'TITAT

AAAATACTGG

AACGAATT

GTAAAGACNA

TTTACGTGCA

CA ITAACCAA CAACCTrGTG

ATCAAAAT=

ATIACAAAC

AC;ATTCGGGC

AACTAAACAA

AGGAACCCGT

TGGTI'TAGCA

GGAILACCCGT

AATTTCGCGG

ATITACTTAC

ACACATCCCT

TCA.AACTGGC

GAATGGCACA

CATAGTTITA

AGGTGAAACC

CCATGTATCT

TGATCGAT

TTCAAGTGTT CGTGGAACAT EF103-4 (SEQ ID NO:400)

HAANPNSATA

TRGDKATKIL

DECTFFVTGD

NLGKHQNNGQ

SGTDWQGTRV YDAAGNDLTA ENANFIGLA( YDGETGFYEF FDKNTGETRG GTKRILISRT QNYQAVVDLT EVSKDXFTYK RLGXDKLGND VEVYVEHIPY 200 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

HGKKLAFTNG RE-ALTWQTGK IVTNKSGDKI LGTTLWNGTK VVDKNGNDVT AANQNFISLA KFDPNTSKYE FFNLQTGZTR GDFGYFQVVD NNXIRAHVSI GTNRYGAALE LTELNNDRFT YTRMGKDNAG NDIQVFVEHE PYQGTYMPA EF104-1 (SEQ ID NO:401) S S 'S 55 S 5

S

.55SS

S

S

S

*5 5

S.

S.

TGAAAGGGGA

GCACAAAATT

GAGACCACTG

T'ITAGCAACAA

ACACAGGCGA

GAGGACAAGG

GATGAACTAC

GAGGTTCACC

AACACAGC'IT

TCAACTGTCA

AAAACATTAA

AAAAGTTATC

AAAAAAACCT

ACCAGAACGA

AATCAAACAT

AACTTGGAAC

ACGCAAACCT

ATTGCTGTAA

ACAATTTATT

ACAAAAATTG

ACTGCTAAGA

TATATCAGTT

ACAAAAGTGG

AAAI'TACGG

AAAACTGAAG

ATTTCTGATT

GTAATTGCTC

GTTGTTAATC

TCATCAAGAA

ATTAAAGTAA

TACAAGTI'AG

ACTACGCCAA

TTGCCAAAAG

TTWAACTCCAG

CAAAGTAGTA

GTCCGTACAA

GATCAGGTAG

ATGACGCCAA

ACGTTTGATT

TGGATCGACG

AATGAAGGCT

CAAGCGGCGA

GATAATAAAA

AATGCTCAAA

TTAGAGAAGA

ATTACGATI'G

ATCGACTCTG

ATCACATTGA

TTAGTATGALA

TCGGGTTGC

ATACAGCAAA

CGACTACTTC

GCACAACAC

ACACCTCACT

TGACAGTAT

GCGAATTAAA

ATGCACCTGG

GCGGAGAAGT

GTATTACCTT

TAACAACAGA

ACTCG TIGA

CCGGGTTAGA

TAGAATT

CTCAAGTr=r

TGTTAACACC

CTGTTCCAAA

TAGAGAGTGC

GGTCAATTTC

CGAGTCAAAC

TTCAAAGCAA

GTCAACAAAT

CGTATGGTCC

GTGGCAAGCT

TAACAATGGA

CTAATAAAGC

CTITrGAATGC

CAACTGTCTC

AGCATCCTAA

GA.ACGGATTA

TAACCAACGA

ATAAAAGTAT

TTGATACGAC

AAAATCAATT

AAATTCCAGC

ATTCAATTTA

ACAGCCCTCG

TTGGAAAAAC

TGCCAACTCT

CTGCTTCGGT

ATCCAACATT

CACATCGTGT

TCGATTTGAA

CTACAAACGG

AATACAATAC

AA.ACATTGAA

AATTCTCAGA

GAAAAAAACT

CGTAAATGCC

AAAAGAGGCA

AGAAATGAAT

TTCCAGTGAT

TAATGAAAAA

GAGTCTTGCG

TACAACACCG

ATCAGTTI'AT

GACAGGCATT

TAATCAACGA

GGCGGAACCA

=FATATGAA

TGGCGAAATT

AACAACAGAG

TATGCTGTAA

GAGTTATCGA

CAACCAACTG

GCTGCTACAC

GCf CTGC CAG

CCGATTGGTG

GTAACCGCTA

ACAGTTCAAT

GGCGCAACTT

GTTTCAGATA

TTAATCAAAA

CAAGTGGAAC

TTT-TATAAT

ACGCCAGCGC

CA.ACGACAGA ACCACAAACA ACTCAAC!ACC ATCTTTACCT

CAACAACTGA

CATCTGAACA

ATGTTCAAGC

GAACAGAATA

CGTTCCAATT

TACCAGAACA

GGGCAGTCGA

CTTCC TTTAA

TTGAAACTCC

CAATTCAATA

TAGACCGGAC

CTGTCTTTIGG

ATGGTCAAAT

GCGATAATC

ATTCCTTATC

ATCGCAAACC

ACAAkACAACG

GCCAATTACA

CAGCCAAACA

TGCTGTTGGA

'r=AGGTTAT

TGCGGCGACC

AGTAGAACTA

AGGAAAAAAT

TAACGAACGA

GTTAACTlCGC

AAAACAAGAT

=TACCAGAA

ACTCGGGCGG

CATTAATCGG

CAGFTACGAT GTCGACATTA TGGCAAAGAT TATACATTAA CATGAATCAA CAAAAACCCT TTCGGACTAT AACTACTTAT AT7CGCAGCA GTATCCAACA TTTGAAAAGT ACGACAGGAA AAGTAAACTA ATTGCTGATC

AGGGAAATAT

CGTATTAGAG

TTTATATGAA

CACTTAACG

TTTTGACAAG

CATTCAATTA

TGAAACTGCT

AGTTATGGGA

TTATCTTTICA

TACAGTAACG

AATCCAAATTr

CCCAGTCGAT

AGTAACTACT

TATGTAACAA

AGTACAAACG

AATGTAAAAT

GCCACAAA.AC

AAGGACATTA

GTATCCGATC

TGGGGTA-AT'

AGCAAAGAGA

T'TACGAGCTA

CCAACCTCAG

CCAM'I'GGTT

ACGATACCGA

AATAGTAAGC

TTTTCI'TTG TGATGTTGAG TATACTTCTC

CTAAACAAAC

GTGAAATGCG

TTATGGCAC

GTCAAGAAAT

TGGAAGACTA

ATAGCTATTT

ATCTATCATT

AATATATrGA

ATGATCAAAA

AACCGATTCA

CAAAAGAAAT

ATGGTrCAGT

LTAATTATGT

TAACAATGAG

GTGGTTCTGA

ATTTTGA

ACATI'ATGA

GTCAATACTT

ACCAAAATAC

AGTATAAAAA

AAGAACCACA

TAGACATTTT

CAGTAACAAC

TAACACCAAA

TCCCAGAAGA

ACTATACAT

AAATCTATAC

AAAAAGTCAC

TTTATTTAGC

AACCGATTTT

TAGTATGTCC

GTTAACAGAA

TAAGAATCCT

TTPTGATATT

AAGAATAAAT

AAGTACACCT

ACCAATTAGT

TG-GTGCCTAT

AAATTTAGAA

TTATTTTTAT

TA'ITAAGTTC

GCCAGATAGT

TGCTGAAGGT

ACGAACACE I

CTCACTAAGC

TGTrrCAAAC

TGATAALACCA

CAATACTT1AC

CGCCAATGGC

ATCGAATAAT

GAGTGCAACA

AAAAAATATT

AGGCACAACC

TGCTTATTCA

CACAGAAAAC

AGAAACAACA

CACTGCGCCA

AACTGCCACA

CCTTGTCAAT GCACGAAATG TGGCGCCGAT GTTCTTTTTG

TCCACAATAC

ATATCCAACG

CAACAAACGT

TTATATAACA

TI'CTGTTCA.A

AAAAAATGTA

GAAAAATC-CA

TTCTATTACC

TGCGAAAGTC

GGTCTCTGCA

CCAGATGTCT

AGGTGATGCG

TGGGACC.GCG

ATTACTTTTG

TACATTATIG

GGGACAGCGA

A.ATCAAGCGT

ACAAAA.ACGA

ACGATTGAAT

GTGAAAGGCG

ATI

5

ITAAAG

AACGCTGGCC

GCTACCAAGA

GAAGGTATI'

201 TABLE 1. Nucleotide and Amino Acid Seqeuences; of E. fiacalis Genes.

?I'CTACACGC

ATTGATAATG

=TGAT-GCCA

TCCGTGGATG

CTAAAAATTC

ATTTATGTCG

GGTCAAGACG

GGCG'FTTATC

AAACCCGACC

AAACCAGAAG

AAAAFI'GATG

AAAAATGGCA

GTTAAAGATA

GCAACAGACA

GATACTAGCA

GCTAATGTGA

GTGGGCGATA

GATGTCCCGT

TATGAAATTG

GACAGTCAGT

GATAACTTCG

GTTCAGGGAA

ACAAAAGAAG

ACAACGATT

AAAACAGGTC

ATAACGTAGA

TCACGCATAC

TCATGACAGG

AAGAGTATTT

CATTAACGAA

GCGATTCATG

TTCCCTI'CGA

CAATTATTTA

AATCTAAG PP

ATAATTTCGT

TTCAGGGAAC

NAAAAGAAGC

CAACGATTI'A

AAACAGGCCA

AAGCAGGCGT

CTGTCAAACC

AATGGGAACC

TTGAAAAAAT

TCTATAAAAA

TAGAGGTCAA

TTTCCGCGAC

CAGTr.AATGT

CGAAAGCAAT

ATGTCGGCGA

AAGATGTCCC

GGATGAAAAC

AGCAACCGAA

TGATI'ATACT

GACACGPAAA

AACAATTGAT

GAAACCAGAA

AAAAATCACT

CAGTGACGAA

AGAGGTCAAA

TTCAGCGACA

AGTGAATGTT

GAAAGCAATC

TGTTGGTGAT

AGACGTTCCG

TATCCAATC

CGACCAATCT

AGAAGATAAT

TGACGTTCAG

TCGCACAAAA

AGATACAACA

AGACAAAACA

TGATAAAATA

CGTI'CATGTC

TTCNTGGANA

ATTCGAAAAA

CAAGCAATTG

=TACAACAG

'TrGCGAGTAA

GCCATTAAGC

CACAGTCGTT

GAGAACTTTG

GTTTCAGGTC

GGTAAAGAAG

GATACAACGA

GACAAAACAC

GATAAAATAG

Ga7-CATIGTCC

TCGTGGAAAC

TTGAAAAAA

GTI'TACAGTT

AAGTTAGAGG

TTCGTTTCAG

GGAACAGTGA

CAAGCGA.AAC

CGAAGGTTTC

ATGAAAAAGG

CGAATGT.ACC

TSCGTCAXAGG

TACAAGTCAA

MIrCAGCAAC

AACTTGATAA

AAACAGCCTA

TI'ATGTTGG

GTCAACAC-GT

GCGAF1'ATGA

GTGATGACAG

CAGAACATAA

TCACT-CT'FC

ACGAAGGTAA

TTAAkAGATAC

CAACAGACAA

ATG=GATAA

CAATCGTTCA

TTTTGAACTA

TCAATACTCC

GCAGGA.ATAT

AGACAACCAA

AGATTCAACG

AGATAAAACA

CANCAAAGCA

TGTGACCGTC

TGATTCGTGG

NCCGTTTGAA

AATTGTCTAT

TCAG FTAGAG TTITCG ITTCA

AGGTCAAGTT

AGAAGAAACA

AACGATTTAT

AACAGGTCAA

AATAGGCGAT

TGTCCGTGAT

GGAAGCAGAA

AAAA.ATTGAT

TAAAAATGGC

AGTCAAGGAT

AGCNACAGAT

ATATGTCG GTGATAAATC GGTCAAGACG TTCCGT=GA GGCGATTATG AA.ATTGTCTA CGTGATGATA GTCGI'TACA CCAGAAGNGA ACTTTGTTTC

ATCACTGT

9* 9 9 99 9.

9 9 9.

99 9 S 99 9.

9 999 9 9a *9 9

S

9 *99~

C

99*9 EF104-2 (SEQ ID NO:402) MKKKTF SFVMLSILLA QNFGFAVNAY AVTTTEAQTE TI'DTAKXEAE- LSNSTPSLPL

ATTTTSEMNQ

ELLDSMSLAP

TVSGEVTG IG

KTYSFDLYEQ

LEPQVFSYDV

IYLESASDYN

ISFQSKGXYY

TEGGKLTLTA

VNPLNAETAW

KLGTDYTVTP

TP VOTTVTTN QVDS IYPQYN

IDVPTLYITG

NKTHRVKNPT

T IEYNT'JSAN

YTHNVEDENQ

VDEEYLTGKA

QDVPFEKITV

PEDNFVSATD

KDTTIYVGDS

NVWVKPDQSK

E IVYKNGTKE QG IVNVDKIG

TGQDVPFEKI

PTATTESQTT

IGGTEYSQTE

ATWAVDAATK

VEPIQYNERT

DINGQILPET

YLYSQQYPTT

VTIYGTLTET

TKDSYLRINI

GNYDQNGAYS

TSDGSVIKFT

SKRGSERTLQ

DRGQYFDKPM

TAKEPQSNNN

IELTPKGTTN

AGQIYTETTI

AIA.KVSFELI

IKLVKGDNQL

SGQVDNXKAG

KTGQDVPFEK

WKPEDNFVSA

LEVKDTI'IYV

AKAIVHVRDD

DYE IVYKNGT

TV

EASTTASSDA

VHRELNT'PV

TLSITFNQRV

RTTGLOGEIF

QTLLTPGKDY

KICSISLKST

KVGQQIVLES

SDLTMDFDKK

SRTTVSVMGS

TPITNEIQIP

SSKNQFLVNA

TPNSPGYPTI

ECSASVSVQN

AQIDLNS ITV

DSETLNQMSA

DNV'THTATEF

KIPLTKTIDH

VYPI IYSDEG

IDVQGTVNVD

TDKTGQDVPF

GDKWEPEDNF

SQLMVDTI

KEAXKAIVHVR

ATPSEQQTTE OKDTSLNEKA TATFQFAVGN TGYAPGSVYT SDTSFKV-L( SYLTI'EAEPL YNLDRTLTGN QTLELLTI'ET TLSDNSLGRI AVTVPNMNQQ TGTKQTTDFT AXTSQTSKVI TNGQE IKNPK

DINLSLSTPV

I<EKPIQNLEI

IGFNYVPD)SL

RNDSFDSLSV

TFDENTNSYT

EALDILSATQ

KGVPEDAYSL

SKXKVITAP I

?TDEKGQYSF

SRLQVKDSTI

KEETAYVTVK

RIGDYEIVYK

EKITVSGQVD

VSATDKTGQD

YVGDKWE-AED

DDSRLQVKDT

FTAYGPLYEN

IGNKAIQLV

KVKHPNYLSL

PKDKSIPVDT

RTXIPAGADV

FDFGKTNKRY

AANPTLKNVr

EKTTNCAXVI

TLKFSEGDAE

DAIMTGDYTL

YVGDSWKPEE

PDQSKLEVKD

NGXKEAKAIV

TSKAGVYPIV

VPFEKIDVQG

NFVSATDKTG

TIYVGDSWXP

LPDVQAPITD

VQLPEHLGYS

IKIETPGKNK

PGAVFGKQDN

KAYSLSINRT

ADREMRSMSY

VKLEDYFDIK

SDQYIEPISV

RATKE IYFYY

IPITMSAEGL

LFD IYD)VSND I IEYKNANGW

KTTVTTKNID

FKDYTLTENI

GIVYLATATF

RVTNVPQEYS

NFVSATDKTG

TTIYVGDSWI<

HVRDDSQLE:V

YSYEGKEETA

TVNVDKIGDY

QDVPFEKIDV

EXNFVSATDK

9999

S

S. 9 99 a 59 202 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faccalis Genes.

EF104-3 (SEQ ID NO:403) TGTA.A CAACGACAGA AGCACAAACA 9.

9 9 99 .9 9 9 9 9 99 99 9. 9 9.

99 9 999 9 9 9@9999 9 .99.

9 9999 9.*9 9 9

GAGACCACTG

TTAGCAACAA

ACAGAGGCGA4

GAGGACAAGG

GATGAACTAC

GAGG?1'CACC

AACACAGGTT

TCAACTGTCA

AAAACATTAA

AAAAGTTATC

AAAAAAACCT

ACCAGAACGA

AATCAAACAT

AACTTGGAAC

ACGCAAACCT

ATTGCTGTAA

ACAA'ITTATT

ACAAAAATG

ACTGCTAAGA

TATATCAGTT

ACAAAAGTGG

AAATTTACGG

AAA.ACTGAAG

ATTTCTGATT

GTAAITGGTC

GTT-GTTAATC

TCATCAAGAA

ATTAAAGTAA

TACAAGTTAG

ACTACGCCAA

2TTGCCAAAAG

TTAACTCCAG

CAAACTAGTA

GTCCGTACAA

GATCAGGTAG

ATGACGCCAA

ACGTITGATT

TGGATCGACG

AATGAAGGCT

CAAGCGGCGA

GATAATAAAA

AATrGCTCAAA

TTAGAGAAGA

ATTACGATTG

ATCGACTCTG

ATCACATTGA

TTCTACACGC

ATTGATAATG

T'ITGATGCCA

TCCGTG.GATG

CTAAAAATTC

PLTACAGCAAA CGACTACTTC A~ GCACAACAGC T ACACCTCACT T TTGACAGI'AT C GCGAATTAAA2 ATGCACCTGG IZ GCGGAGAAGT C GTATTACGTT2 TA.ACAACAGA C

ACTCGTTTGA

CGGGGTTAGA

TAGAAT 'ATT

CTCAAGTTTT

TGTTAACACC

CT-GTTCCAAA

TAGAGAGTGC

GGTCAATI'TC

CGAGTCAAAC

TTCAAAGCAA

GTCAACAAAT

CGTATGGTCC

GTGGCAAGCT

TAACAATGGA

CTAATAA.AGC

CTTTGAATGC

CAACTGTCTC

AGCATCCTAA

GAACGGATI'A

TAACCAACGA

ATAAAAGTAT

TTGATACCAC

AAAATCAXI-r

AAATTCCAGC

ATTCAATTIA

ACAGCCCTGG

TTrGGAAAAAC

TGCCAACTCT

CTGCTTCGGT

ATCCAACATT

CACATCGTGT

TCGATTTGAA

CTACAAACG

AATACAATAC

AAACATTGAA

AATTCTCAGA

ATAACGTAGA

TCACGCATAC

TCATGACAGG

AAGAGTATT

CATTAACGAA

LAAAGAGGCA G CGAAATGAAT C ~TCCAGTGAT G ~AATGAAAAA G AGTCTTGCG C ACAACACC GC ~TCAGTTAT A~

;ACAGGCATT

'AATCAACGAC

~GCGGAACCA I E1'TATATGAA C rGGCGAAATT I kACAACAGAG I~ :AGTTACGAT C rGGCAAAGAT

CATGAATCAA

TTCGGACTAT

m'GAA.AAGT

A.AGTAAAGTA

AIGGGAAATAT

CGTATTAGAG

=TATATGAA

CACTTTAACG

TITTGACAAG

CATTCAATTA

TGAAACTGCT

AG2ITATGGGA

TTATC=TCA

TACAGTAACG

AATCCAAATT

CCCAGTCGAT

AGTAACTACT

CCTTIGTCAAT

TGGCGCCGAT

TCCACAATAC

ATATCCAACG

CAACAAACGT

TrATATAACA

TTCTGTTCAA

AAAAAATGTA

GAAAAATCCA

TTCTATTACC

TGCGAAAGTC

GGTCTCTGCA

CCAGATGTCT

AGGTCATGCG

GGATGAAAAC

AGCAACCGAA

TOATTATACT

GACAGGAAAA

AACAATTGAT

AGTTATCGA A AACCAACTC C CTIGCTACAC C CCCTGCCAG A :CGATTGGTG G ;TAACCGCTA C LCAG T--CAATI ;CGCAACTT G ;TTTCAGATA C ~TPATCAAAA 'I AAGTGGAAC C 'TTTATA.ATT 'I ~CGCCAGGCG C

TCGACATTA

CATACATTAA C AAAAAGCCT .ACTACTTAT I k.CGACAGGALA C kTTGCTGATCC IATGTAACAA2

%.GTACAAACG

AATGTAAAAT2

GCCACAAAAG

AAGGACATTA

GTATCCGAtC

TGGGGTAATT

A~GCAAAGAGA

TTACGAGCTA

CCAACGTCAG

CCAATTGGTT

ACGATACCGA

AATAGTAAGC

GCACGAAATG.

GETTTTTG

TGGGACCGCG

ATTACT1'TG

TACATTATTG

GGGACAGCGA

AATGAAGCGT

ACAAAAACGA

ACGATTGAAT

GTGAAAGGCG

ATTIW-TAAAG

AACGCTGGCC

GCTAGCAAGA

GAAGGTATTG

CAACCA.ATTG

TTTACAACAG

TTGCGAGTAA

GCCA2LTAAGC CACAGTCG2T

.CTCA.ACACC

AACAACTCA

ATCTGAACA

.TGTTCAAGCC

AACAGAATAC

:CTTCCAATT2 'ACCAGAACA2 ;GGCAGTCGA2

:TTCCTTTAA

~TGAAACTCC

:AA'IrCtAATA

'AGACCGAC

TGTCTTTGG

~TGGTCAAAT

;CGATAATTC

.TTCCTTATC

~TTCGCAGCA4

TAA.ACA.AAC

;TGAAATGCG

=rATGGCAC

;TCAAGAAAT

CGGAAGACTA

kTAGCTA II

%TCTATCATT

N.ATATATI'CA

A.TGATCAAAA

.AACCGA7TCA

CAAAAGAAAT

ATGGTTCAGT

TTAATTATIGT

TAACAATGAG

GTGGTTCTGA

ATrCTTTTGA ACA ETTATGA

GTCAATACTT

ACGAAAATAC

AGTATAAAAA

AAGAACCACA

TAGACATI=

CAGTAACAAC

TAACACCAAA

TGCCAGAAGA

ACTATACATT

AAATCTATAC

AAAAAGTCAC

TTEAT'FTAGC

CGAAGGTTTC

ATGAAAAACG

CGAkATGTACC

TGGTCAAAGG

TACAAGTCA-A

4TCI=ACCT

~TCGCAAACC

~CAAACAACG

;CCAATTACA

AGCCAAACA

C'CCTGTTGGA

[TTAGGTTAT

rGCGGCGACC kGTAGAACTA

AGGAAAAAAT

rAACGAACGA GTTAACTG-cC

AAAACAAGAT

TTTACCAGAA

ACTCGGGCGG

GATTAATCGG

GTATCCAACA

AACCGATT'Fr

TAGTATGTCC

GTTAACAGAA

TAAGAATCCT

TTTTGATATT

AAGAATAAAT

AAGTACACCT

ACCAA2LTAGT

TGGTGCCTAT

AAAT ITAGAA

TTATTTITAT

TATTAAG LTC

GCCAGATAGT

TGCTGAAGGT

ACGAACACTT

CTCACTAAGC

TGTTrCAAAC

TGATAAACCA

CAATAGTTAC

CGCCAATGGC

ATCGAATAAT

GAGTGCAACA

AAAAAATA2TT

ACC-CACAACC

TGCTTATTCA

GACAGAAAAC

ACAAACAACA

CACTGCGCCA

AAcTGCCACA TWrTGAACTA

TCAATACTCC

GCAGGAATAT

AGACAACCAA

AGATTCAACG

203 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.fJaecalis Genes.

ATTTATGTCG

GGTCAAGACG

GOCGIATC

AAACCCCACC

AAACCAGAAG

AAAATTGATG

AAAAATGGCA

GTTAAAGATA

GCAACAGACA

GATACTAGCA

GCTAATGTGA

GTGGGCGATA

GATGTCCCGT

TATGAAATTG

GACAGTCAGT

GATAACTTlCG

GTTCAGGGAA

ACAAAAGAAG

ACAACGATT

AAAACAGGTC

GCGATTCATG

TTCCCTTCGA

CAATTATTTA

A.ATCTAAGTT

ATAATTTCGT

TTCAGGGAAC

NAAAAGAAGC

CAACGATIA

AAACAGGCCA

AAGCAGGCGT

CTGTCAAACC

AATGGGAACC

TTGAAAAAAT

TCTATAAAAA

TAGAGGTCAA

TTTCCGCGAC

CAGTGAATrGT

CGAAAGCAAT

ATGTCGGCGA

AAGATGTCCC

CAAACCAGAA

AAAAATCACT

CAGTGACGAA

AGAGGTCAAA

TTCAGCGACA

AGTGAATGTT

GAAACCAATC

TGTTGGTGAT

AGACGTTCCG

TTATCCAATC

CGACCAATCT

AGAAGATAAT

TGACGI'CAG

TGGCACAAAA

AGATACAACA

AGACAAAACA

TGATAAAATA

CGTTCATGTC

TTCNTGGANA

ATTC

GAGAACT=G

GTTCAGGTC

GGTAAAGAAG

GATACAACGA

GACAAAACAG

GATAAAPLTAG

GTTCATGTCC

TCGTGCAAAC

TTTGAAAAAA

GTTACAGTT

AAGTTACAGG

TTCCTTTCAG

GGAACAGTGA

GAAGCGAAAG

AATATGG

GGTCAAGACG

GGCGATI'ATG

CGTGAT--ATA

CCAGAAGNGA

TTTCAGCAAC

AAGTTGATAA

AAACAGCCTA

TI'TATGTTGG

GTCAAGACGT

GCGA LPATGA

GTGATGACAG

CACAAGATAA

TCACTGTTC

ACGAAGGTAA

TTAA.AGATAC

CAACAGACAA

ATGTTGATAA

CAATCdTTCA GTGATWATG3

TTCCGTTTGA

AAATTGTCTA

GTCGTTTACA

AC'TTGTTTC

AGATAAAACA

CANCAAAGCA

TGTGACCGTC

TGAITCGTGG

NICCGI'GAA

AA ITGTCTAT

TCAGTTAGAG

TTTCGTTTCA

AGGTCAAGTT

AGAAGAAACA

AACGATTTAT

AACAGGTCAA

AATAGGCGAT

TGTCCGTGAT

GGAAGCAGAA

AAAAATTGAT

TAAAAATGGC

AGTCAAGGAT

AGCNACAGAT

EF104-4 (SEQ ID NO:404) VTTTE-AQTE 'ITDTACKEAE LSNSTPSLPL 0@ 00 S 0S

S.

00 6 00 @0 5 5 @0

S.

@0 S 0@0 0

S.

0@ S 000 0 6 000.00

S

05., 5 @600

S.

0050 0 0050 0000 0000 0 0500 OS 0 00 50

ATTTTSEMNQ

ELLOSMSLAP

TVSGEVTGIG

KTYSFDLYEQ

LEPQVFSYDV

IYLESASDYN

ISFQSKGKYY

TEGGKLTLTA

VNPLNAETAW

KLGTDYIVTP

TPVDTTrVITN QVDS IYPQYW

IDVPTLYITG

NKTHRVKNPT

TIEYNTVSAN

YTHNVEDENQ

VDEEYLTGKA

QDVPFEKITV

PEDNFVSATD

KDTTIYVGDS

NVTVKPDQSK

EIVYKNGTKE

QGTVNVDK(IG

TGQDVPF

PTATTESQT

IGGTEYSQTE

ATWAVDAATK

VEPIQYNERT

DINGQILPET

YLYSQQYPTT

VTIYGTLTET

TKDSYLRINI

GNYDQNGAYS

TSDGSVIKFT

SKRGSERTLQ

DRGQYFOKPM

TAYEPQSNNN

IELTPKGTTN

AGQ IYTETTI

AIAKVSFELI

IKLVKGDNQL

SGQVDNXKAG

KTGQDVPFEK

WKPEDNFVSA

LEVKDTTIYV

AKAIVHVRDD

DYEIVYKNGT

EASTTASSDA

VHRELNTTPV

TLS ITFNQRV RTTGLDGEI F

QTLLTPGXDY

KIGS ISLKST

KVGQQIVLES

SOLTMDFIDKK

SRTTVSVMGS

TPITNEIQIP

SSKNQFLVNA

TPNSPGYPTI

EGSASVSVQN

AQ IDLNSITV

DSETLNQMSA

DNVTHTATEF

KIPLTKTIDH

VYPIIYSDEG

IDVQGTVNVD

TDKTGQDVPF

GDKWEPEDN"F

SQLEVKDTTI

KEAKAIVHVR

ATPSEQQTTE

TATFQFAVGN

SDTSFKVELK

YNLDRTLTGf4

TLSDNSLGRI

TGTKQTDFT

TNGQEIKNPK

DINLStSTPV

KEKPIQNLEI

IGFNYVPDSL

RNDSFDSLSV

TFDENTNSYT

EALDILSATQ

KGVPEDAYSL

SKKKVITAP I

TTDE-KGQYSF

SRLQVKDSTI

KEETAYVTVK

KIGDYEIVYI(

EKITVSGQVD

VSATDKTGQD

YVGDKWEAED

DDSRLQVKDT

DKDTSLNEKA

TGYAPGSVYT

.SYLTTEAEPL

QTLELLTTET

AVTVPNMQQ

AKTSQTSKVI

FTAYGPLYEN

IGPNKAIQLV

KVKHPNYLSL

PKDKS IPVDT RTKI PAGADV

FDFGKTNKRY

AANPTLKNVT

EKTTNGAKVI

TLKFSEGDAE

DAIMTGDYTL

YVGDSWKPEE

PDQSKILEVKD

NGXKEAKAIV

TSKAGVYPIV

VPFEKIDVQG

NEVSATDKTG

TIYVGDSWXP

LPDVQAP lTD

VQLJPEHLGYS

IKIETPGX(NK

PGAVFGKQDN

KAYSLSINRT

ADREM'RSMSY

VKLEDYFDIK

SDQYIEPISV

RATKEIYFYY

IPITMSAEGL

LFDIYDVSND

I IEYXNANGW K'FIVITKNI U

FKDYTLTENI

GIVYLATATF

RVTNVPQEYS

NFVSATDKTG

TrIYVGDSWK

HVRDDSQLEV

YSYEGKEETA

TNNVDKIGDY

QDVPFEKIDV

EXNFVSATDK

EF105-1 (SEQ ID NO:405) TAAATGAAAA AALACAGTCGT CTACTCCTTG TTAIMCGAA CAATGTTGCT TGGCGCCAcT GTTCCTGCC AAGCGGCGAC GGTCG TTII GATAGCGAAC AGTCGATTGT TTTTACCCCA ACCACAGATO GGACGGATCC AGTAAATCCA GAAAATCCCG ATCCAGAAAA ACCAGTTCGA 204 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faccalis Genes.

CCAGTCGATC

GATTATGCCT

TTTGCCAGAG

AATTATGTAC

CAA.AATGGTC

GCCTTTACTG

GCAAACA LTC

ACCGGCGCCG

CAACAAGGAC

AAAACCCCTA

CCAGTAAATA

CAACGAATCC

CAAGTTTGGA

CGCAAACCTA

AAGTAAGTGA

AATTTCGTAA

AGCCCAGTGT

AATTAGATCC

GAACGTGGAT

AGCAAGTAAA

AGGATGCAGT

ATCGAGGGAA

TGATGGACCT

TTTTGGGAGT

TAGAAATCCA

'ITTIACGGGGA

TGCAGAAACA

TCGCTCAAAT

TGCGGGCGCA

CACGCTGTCG

TGCCACAATC

ACAATATAAA

ATAA

AATCCAGGTA

AATGAGATAT

GATGr.TTCAG

ACCAATGCTG

TTACACA.ZAG

GCGACGGACG

GAAACTG=I

GGGCAAGCAG

ACACGGGCA-A

ACAACATTGA

CCCCTGGTCC

CGAATAAGGA

CAAGTGAATT

GCTGGGTTTT

AA ITAACAGG

TATTGCCGCC

TCATGCAAGC

AAAAAGTC.AC

TCTCACTAAC

CTTGGCTACT

ACTI'TCCATC

TCAAACGTAT

GGCAACTGCT

AAAAGTGAAA

CGCCACCOTC

AACTGCTACC

CCCAGAAAAG

CCAA.AAAAAT

TG ITCCTGGG rICAGATGTA EF105-2 (SEQ ID NO:406)

MYKKVVYSLL

VDPTNPDGPN

YVQVSDLRGT

NI QLDAAGAE

TPKDAVQYKT

FGTMLLGATV

PGTPGPLS ID NAG WVLKVKQ

TVVMQAPEKT

TLTWLLSDVP

PAEAATVVFD

YASSLDFGSN

NGQFRNAETL

GAGTWITLWG

VNNGGF

SEQSIVFTPS

El SNKDQTYF

HKELTGATVA

QAEKVTEKNQ

TOGTDPVNPE

ARAZQTYRNPD

FTEPSVRSNA

QGQQVNATIT

NPDPEKPVRP

GSASELATAN

TDVLPPTATA

RAISLTVPCK

o 0 0* 0 000 0 0*0* *000 EF105-3 (SEQ ID NO:407) GCCGAC GGTCGT'ITT GATAGCGAAC AGTCGATTGT TTTTACCCCA

ACCACAGATG

CCAGTCGATC

GATTATGCCT

TTGCCAGAG

AATTATGTAC

CAAAATGGTC

GCCTTTACTG

GCAAACATTC

ACCGGCGCCG

CAACAAGGAC

AAAACCCCTA

GGACGGATCC AGTAAATCCA CAACGAATCC TGATGGACCT CAAGTTTGGA TTTGGGAGT CC-CAAACCTA TAGAAATCCA AAGTAAGTGA TTACGGGGA AA =TCGTAA TGCAGAAACA AGCCCAGTGT TCGCTCAAAT AATI'AGATGC TGCCGGCGCA GAACGTGGAT CACGCTGTGG AGCAAGTAAA TGCCACAATC AGGATGCAGT AC GAAAATC CCG

AATCCAGGTA

AATGAGATAT

GATGGTTCAG

ACCAATGCTrG

TTACACAAAG

GCGACGGACG

GAAACTGTTG

GGGCAAGCAG

ACACG.GGCAA

ATCCAGAAAA

CCCCTGGTCC

CGAATAAGGA

CAAGTGAATI'

GCTGGGTTTT

A.ATTAACAGG

TATTGCCGCC

TCATGCAAGC

A.AAAAGTGAC

TCTCACTAAC

ACCAGTTCGA

ACTTTCCATC

TCAAACGTAT

GGCAACTGCT

AAAAGTGAAA

CGCCACC-GTC

AACTGCTACC

CCCAGAAA-AG

CGAAAAAAAT

TGTTCCTGGG

EP105-4 (SEQ ID NO:408) no.

0000 0 00 ATVVFD SEQSIVFTPS TDGTDPVNPE NPDPEKPVRP VDPTNPDGPN PGTPGPLSID YASSLDFGSN EISNKDQTYF YVQVSDLRGT NAGWVLKVKQ NGQFRNAETL HKELTGATVA NIQLDAAGAE TVVMQAPEKT GAGTWITLWG QAEI<VTEKNQ

TPKDAV

ARAQTYRNPD GSASELATAN FTEPSVRSNA TDVLPPTATA QGQQVNATIT RAI SLTVPGK EF106-1 (SEQ ID NO:409)

TAGTCGTTTA

TTAGTTGGC

AATCCCAATA

GAAGGAFI'AT

TTAACAGATG

TATGAT=T

ACCGCTGGTA

GGTGTATACC

GATAAAAACG

TGAAGAAAAA

GAGCAGGAGG

ATTTAGGGGA

CAGAAAAAGA

AAAATCAAAA

TTGATAAAGA

TTACAGATCT

CAC1'TAGAAC

GAAATGATAT

AATCGTTGGT

GGCTTTrGACG

TTACCMCAG

TT1GGGCTGGA

CCTATTACAT

GACTIGGAGAA

TTCCCGTCTT

TTTATACCAA

TGAAGTTI'C

ACAATTACGT

GCAGAAGCAT

TATTTACGTT

ACACGCGTT

GCAATCAAAT

TCAACAGGAG

GTAA'TATT

GATACTTTI'A

GTAGAAAACA

TGTTCCCTTT

ACGTICCTCA

CAG~TTGGTAT

ATGATCGAAA

TTG-ATGCAAC

ATGAAGGAAC

CTGAAACCAA

CGTATAGACA

A.AGCAACCTC

AAGTGCGTNTA

AAGCGTAGAC

TAGACAAGAT

TGGGAATGAC

CACTAGTTTC

CTTCT1'ATG

AAATTATCAA

GATGGGGAAA

AGGACCAGTT

1 205 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

TATGGTCGTC

CGCCGTGCCA

ATTGGTAAAA

GATAAAGATG

GACCAAGATA

TATGGCTACT

AATCGCTATG

CGAATGGGTA

CAAGGAACTT

CGCAGCCATA

TGACAGAACA

CT'TCCTI'TGA

GCAATGACGT

GCAGCAAATA

TTAAAGTAGG

GCGCTGTCTT

AAGATAACGA

TTAATCCTGA

CCCCAATAAT

AACAGGCCAG

TGGGACACCG

AACTTCGGCC

Ty-AATT'IIC

AAATCAAAAT

AGAGTTAACA

AGGAAACGAT

ATTTAC=TI'

CGTCCCAGAA

ATTGATGTA-A

CAACTTCTTT

AACCAAAACT

AATTTACAAA

AAATirCCGTc

GAAI'GAATG

ATCCAAGTCT

TAA

CACTAGAAT

ATCGACAAGG

GGAATGGCAC

TTATCAGC IT

CTGGTGAAAC

CCCATGTTTC

ATAATCGTTI'

AtGTGGAACA

CACGAATGGA

GGATGAAATT

AAAAGTAGTG

AGCGAAATT

TCGTIGGCGAC

CATTGGAACC

TACGTACACA

TGAACCATAC

EF106-2 (SEQ ID NO:410) MKXIVGT ITLLALSALL VGGAGGALTA EAYVPQSVDN PNNLGDLPEY LRSVGIRQDE GLSEKOWAGT RVYDRNGNDL TDENQNLLHA IKFDATTSFY EFFDKETGES TGDEGTFFMT AGITDVSRLV IISETKNYQG VYPLRTLYQD TFTYRQMGKD KNGNDIEVFV ENKATSGPVY GRPQPYPNNR PRTLEFTNGR RAMTEQTGQI DVNRQGDEI I GKTSFDGTPQ LLWNGTKVVD KDGNDVTSAN QNFISLAXFD QDSSKYEFFN LQTGETRGDY GYFKVGNQNK FRAHVSIGTN RYGAVLELTE LNDNRFTYTR MGKONEGNDI QVYVEHEPYQ GTFNPEFTF EF106-3 (SEQ ID NO:411)

C

C C

C.

C C CC, C

CC

C C

C

C

C

AT ACGTTCCTCA AAGCGTAGAC

AATCCCAATA

GAAGGATTAT

F1'AACAGATG

TATGAATTTT

ACCGCTGCTA

GGTGTATACC

GATAAAAACG

TATGGTCGTC

CGCCGTGCCA

ATTCGTAAAA

GATAAAGATG

GACCAACATA

TATGGCTACT

AATCGCTATG

CGAATGGGTA

CAAGGAACTT

ATTTAGGGGA

CAGAAAAAGA

AAAATCAAAA

TGATAAAGA

TTACAGATGT

CACTTAGAAC

GAAATGATAT

CGCAGCCATA

TGACAGAACA

C EFCTTTGA

GCAATGACGT

GCACCAAATA

TTAAAGTAGG

GCGCTGTCT

AAGATAACGA

TTTACCTGAG

TTGGGCTGGA

CCTATTACAT

GACTGGAGAA

TTCCCGTCTT

TTTATACCAA

TGAAG=TTC

CCCCAATAAT

AACAGGCCAG

TGGGACACCG

AACTTCGGCC

TGAATITTTC

AAATCAAAAT

AGAGTTAACA

AGGAAACGAT

TATTTACGTT

ACACGCGTT

GCAATCAAAT

TCAACAGGAG

GTAATTATTT

GATACTTTTA

GTAGAAAACA

CGTCCCAGAA

ATTGATGTAA

CAACTI'CTrT

AACCAAAACT

AATTTACAAA

AAATTCCGTG

GAATTGAATG

ATCCAAGTCT

CAGTTGGTAT

ATGATCGAAA

TTC'ATGCAAC

ATG-AAGGAAC

CTGAAACCAA

CGTATAGACA

AAGCAACCTC

CACTAGAATT

ATCGACAAGG

GGAATGGCAC

TTATCAGCTT

CTGGTGAAAC

CCCATGTFI'C

ATAATCG'TI

ATGTGGAACA

TAGACAAGAT

TGGGAATGAC

CACTAGTITTC

CTTCTTTATG

AAATTATCAA

CATGGGGAAA

AGGACCAGTT

CACGAATGGA

GGATGAAATT

AAALAGTAGTG

AGCGAAAT

TCGTGGCGAC

CAITTGAACC

TACGTACACA

TGAACCATAC

*CC.

C. C

C

EF106-4 (SEQ ID NO:412) YVPQSVDN PNNLGDLPEY LRSVGIRQDE GLSEXDWACT RVYDRNGNDL TDENQNLLHA AGITDVSRLV IISETKNYQG VIYPLRTLYQD GRPQPYPNNR PRTLEFTNGR RANTEQTGQI KDGNDVTSAN QNFISLAKFD QDSSKYEFFN RYGAVLELTE LNDNRFTYTR MGKDNEGNDI

IKFDATTSFY

TFTYRQMGKD

DVNRQGDEI I

LQTGETRGDY

QVYVEHEPYQ

EFFDKETGES

KNGNDIEVFV

GKTSFDGTPQ

GYFKVGNQNK

GT

TGDEGTFFMT

ENKATSGPVY

LLWNGTKVVD

FRAHVS IGTN EF107-1 (SEQ ID NO:413)

TAAAAAACGG

ATANATANAA

CTTTATAGAG

AATTGGAAAA

CACTCAATAT

AAATGCTAGT

ACTATAGATT

GATGGCTAGT

GTCAAAATT

TATCAGTATC

GAATTTTTAC

TGTTGGGTTA

GAAATTTCAA

GATAATAACA

ATAGAAAGAA

AGTTTTCTT

GCTGTCGTGTT CTTGGTAAA GGATACTGAT TAAC;AAAGGA GGAGCAAGAT GAAGCdAGTA TGTTCATGGA TTCAGTG 206 TABLE 1. Nucleotide and Amino Acid Seqeuences of Efaecalis Genes.

0**t

GGTGTGACTG

CAGTCGAGTG

GCTAGTGAAG

GGTTATTCFT

TTGTTTGATC

GAGAAATTAC

ATTCCAACGT

GGCGTTTCTG

AGTACGTTTT

GGGGGCAAAG

GAAAAAATGC

ACGGCACCAG

ACTAGTTCGC

CGTGTCACGG

ACCCAGGATA

TTGCCTGAAC

GGGAATGCGA

GATGACAATG

CCAGCGAGAG

AAGTTTAAGC

TTTCCCACAA

CAAAAAGTC'I

AGTGTTGAAA

GGGGATAAAA

AACA ITGTT,

CGCTATACTC

AGTATCTATI

TATTATTAC!

ATCACGCCAC

GTCTACACTC

TTCCAAGGCI)

AGTTTTACA(

CCCACCGCG(

GATTACTGG(

AAGCCAACGC

ACGGGTCAA

GTGTCCATCO

GGAACCGCC(

AACTrrGGC,

ACGAGTCCT,

ATTICAGGA

ACTCGCAAC

CTATCGCAA

ACGGCCGCT

GGCAAGACC

CAACAGT'C

GAACTCCCA

TGTTAGCGGA

ACGAAGCGAG

CGAAAACAGT

CTGTGGAAGG

CCGACCCAAT

AACGAACCCC

CACCAGVITI

AAGTGTCGTT

ATACAGGAGA

TGACGAACTA

CCAATAAGCT

GAGGCGTTAC

ACCAAAGACG

AAAAA TrGA

AGAAAACCAT

GTTACACAGG

AACCTAGCAC

ACGATPGCA

AAGCTTTGTT

TAAGTGCGAC

TTGATGGAAT

ACACACGCCC

TTCCTAAGTA

LCCAAGTATCC

GTAGTTTAGI

CCCGCCGTTC

CAGGAACTGC

7TAGAAAATCC

CAACTGGCTI

TCGCAAAAGC

EGGTTAAAGC

CAACTTTTA;

3AACTAACGT 3AGGCGCTAC'.

3 CAACTTGGG( k ACACCAAAG( k~ CGTTGGAMC 3TTACAGGAA.

N GTTTAACTGI S ACGGCGACGI A GTAAGCAAC, C CTTATTTAC

CAAAATCAG

CTGCCAGCT

A GCACCGTGA A CAGGCAGTG .G CCAACCAAG

AACGATTACT

CCAGACGACG

ACCCCACAG

GcGcG;AGATT

TACGATGGCG

CC=ITAGCCC

TAAATATGAT

TACTATTCCT

CACTACGCAA

TGTGGGCGCC

GGGGCGACGG AGCAAGGAGT CAAACAkACCG AAGAGTCACA GAAACGGCAA GAATTOCTTC CCCTTTTTCT TTGTGGAGGA GTCAATCTTT

CCACGTTTTC

ACCACTGTGA ATGGCGGAAA ACAAATAACC AGAATCCAAG

AAGGAGTATC

TATCCAGTGC

AATGCGGAGA

GACGT-'TTGGT C-TAAG GTATGCGCTG ACCTATTTTT

AGGAACAGCG

GAATCCCGCA

TGTAACAGGG

CAGTGATGGG

GTTGGAAACC

TGTGGTCTAT

GGTCCTGTGT

GGCGGGGCGA

GAGGATTTTA

AAGACGTAT1'

ACCAAAACGC

GAAGAAGCAG

CGGCTATGTT GATGAGCAAC CATGGGTG-AA

AGTGACGGAG

CGATATGCCA C

AGACGATGGG

TTACCAGACG1

AGTACCAAAT

GGGAANCNCT

TTACTGGANG

T GGGGGCAAC

GCGGGTCACT

TACACAAGGA.

'FTGCCGAAG.

cAAAACCAAG

'GATGAGGAC

AACAGGTGCC

GAAGA.ACACA

GGCTGGCATC

TTTTCCTGTC

GCCTTTACCA

TCCTGGTCAA

cAAAGATACG cTrTATTAGT

ATATGGTTTG

CCTGAATACT

CACAGACAGC

TACCGATTAC

TITAACCGCC

CGTCTATCAG

TATGGCTGGC

AGCAACATCT

GGCAACGGTC

TCGAGCGATT

AGACGGGACG

GTTTTATGAA

GTTACTGTCT

TAATATI'TAT

;CAAGTCAAT

~CAATCGTAA

kTTTCGAT=I

'AAGTGCGCC

3CGTATAACT rGGGGCCCCA rATAATTTAT

GAACATTTTG

AATCAGCTAG

ATCTACCAAG

CCAGCAACAT

GACATCACCC

GTI'GACATAA

GGCGAAGCGC

GGCGCACCCA

ACCAAAGAAC

GCTGGCGGTC

GTTI'TAACCC

GTCCGTATT;

ACCCCGACX

A.AGAAGGCCC

AGCCAAGCC

TTGCCAACk

AACCAACCAX

GACAATACC(

TTGGACTTC

CAACAATAC

AAAGCCTGGA CATTCGACCA CAACGGTTTT TGCGAACGTT CGGAATTAGA GTTAACCAAT CGTT.TAAATA TACGGTAGCT ATGGAGATGT CCGCGGTCCA ATTATrIATT AACAAAGCGG TTCCTGCGCC

AGAAGGTTAT

CTTITACCCA AGAAGGCACC TATTTAAAGG TTGGTACAAA CTAGTTIATGC GGTGACCTAT TGATGAAAAC CTATACGTTG GAAACTTGAT TAATCCCGCC CCACAGGGGA

AATGACGACT

TAAAGAAATT AGCCATCCCG C'ITATG-GCCC GCAAGAAGTG CACCAACTAC

TGCGTATACA

GTGGCATCGA

AAACCCCGAC

TGACCCAAAA

AAGTGCCACA

CGAAGACACT TTACTCAATG CGACCCCTGA TGGCACCATT TAGACGAAAG TGGCGCAAAA TGGTGGACAG TGAAAACTAT CTGGTGAAALA AACCTATATC TAAAGACGAC AACGACCCCA CTGTGTACCA AGA.AGCGATT TC:GAAAATGG CGCCACAATG CGTTALACCAC CATTAAAATC ACACCATATT

TGTACAAGGA

AATGGACGAC

CGGTGCAGGA

AATTAAAAAT

GAACTTATTA

CTGATGTTGA

AGTAACGGG-C

AAGAC'ITAGA TCAAGAAATT TTGATTTTGG

TAAACTAGCA

3CAGATTACTA CGGCAATGGC A ATTGGAGTTT AACGGCCCAG SCGACCCGCTT

GTTGCTAGGA

SCAGAAACCAG GACACCACTI' G CAACAGCGGT CGTCGCAAAC A CGTTTGCTAA CATCAAACTA C AAGCCGCCGT

CACGTGGAAT

TTAGTGACTG GCCCCTAA EF107-2 (SEQ ID NO:414)

MKRVN

WKRWLVVGLS CSLFM.DSVVG VTVLAETITG ATEQGvATSQ SSDE.ASQTTQ 'FrEESQATVA SEAKTVPPQE TARIASRAIG YSSVEGREIP FFFVEEDGTL FDPDRITMAV NLSTFSFYEE TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

KLQRTPLJEPT

TFYTGDTTQY

APGGVTYALT

QDKKTIVTGE

DNZDDLHVVYE

PTIDGIDMPA

DKTKYPVPNE

IYSGTAGGNY

YTVAI(ALPK I

TAELTLTGAV

GQNTKAFPVT

FGSLTAKDT'J

RNPYLRLNTS

KTSTVTLTAD

VTGP

TVNGGKLLS I

PVPTVFANVG

YFYGDVGGPT

DPI'FTQEGTL

EAVMXTYTLP

SQLKKLAIPQ

VRRGIENPDN

NLSTPDGTIY

YQAGEKTYIF

DIIENGATMD

KEQWTTGAGV

RIKDLDQEIT

QANWSLTAQL

NTATAVVANQ

PTSPAFKYDT

GKVTbNYVGAN

SSHQRRGTAG

PERYTGSDGK

AREALFGYVD

KVYTRPDDGT

IVSSLVGXXA

YYLENRRVTE

QGWFKGKTKP

YWEALLKNTG

S ITLDQPLPA.

SPDGDGFIST

SQPKSATDSL

QFTGSDVYQL

NNQNPSNIYG

AETELELTNE

PVYYYLTKRR

TYLFKGWY'KG

EQGNLINPAK

IVTYGPQEVS

YNLTQKSATR

HFVDESGAKI

ATLKTTIIP S

EAPLTTIKIK

,GGQLKMNLLG

PFDFGKLAI

PTTTRLLLGT

OFTFANIKLE

VSEVSFTIPX

K4PNKLTFGP

VTEKFENPAG

NAKPSTLET'

FKLSATMGES

VEI PKYYQTI

YTARRSYWXW

TPPTrFTQGN

FTPTFNDEDD

pTAT 'AAG IG

TAVTGNPGQV

SGSKQQYGLK

,AAAAS FTDYN

VPANQGMAGQ

EYQSLDIRPS

KKTFKYTVAT

GAl PAP EGYT

KTPSYAVTYD

DGATGEMTTF

S ISPTTAYTG

GPTKTLYSMS

QLVVDSENYV

MTAVYQEAI P

APNTIFVQGT

LTADVEVTGN

KAADYYGNGT

QPTETRTPLG

QYQAAVTWNL

EF107-3 (SEQ ID NO:415) GG AGCAAGGAGT AGcAACATCT

CAGTCGAGTG

GCTAGTGAAG

GGTTATTCTT

TTGTTTGATC

GACAAATI'AC

ATCCAACGT

GGCGTTTCTG

AGTACGTTTT

GGGGGCAAAG

GAAAAAATGC

ACGGCACCAG

ACTAGTTCGC

CGTGTCACGG

ACGCAGGATA

ITGCCTGAAC

GGGAATGCGA

GATGACAATG

CCAGCGAGAG

AAGTTTAAGC

T'TCCCACAA

CAAAAAGTCT

AGTGTTGAAA

GGGGATAAAA

AACATTGTTA

CGCTATACTG

AGTATCTATTI

TATTATTAC7I

ATCACGCCAC

GTCTACACTC

TTCCAAGGCI

AGTTI'TACAC

CCCACCGCGC

GATTACTGGC

AAGCCAACGC

ACGGGTCAAJl

ACGAAGCGAG

CGAAAACAGT

CTGTGGAAGG

CCGACCGAAT

AACGAACCCC

CACCAGCTTT

AAGTGTCGTT

ATACAGGAGA

TGACGAACTA

CCAATAAGCT

GAGGCGTTAC

ACCAA-AGACG

AAAAATTTGA

AGAAAACCAT

GTTACACAGG

AACCTAGCAC

ACGATTTGCA

AAGCTTTG T

TAAGTGCGAC

TTGATGGAAT

ACACACCCCC

TrCCTAAGTA

CCAAGTATCC

GTAGTTTAGI

CCCGCCGTTC

*CAGGAACTGC

*TAGAA.AATCC

CAACTGGCTJ

TCGCAAAAGC

GGTTTIAAAGC

CAACTTTTAJ

AACTAACGT'

AGGCGCTACr

CAACTTGGG(

kACACCAAAG(

CCAGACGACG

ACCGCCACAG

GCGCGAGATT

TACGATGGCG

CCTTGAGCCC

TAAATATGAT

TACTATTCCT

CACTACGCAA

TGTGGGCGCC

GACGTTTGGT

GTATGCGCTG

AGGAACAGCG

GAATCCCCCA

TGTAACAGGG

CAGTGATGGG

GTTGGAAACC

TGTGGTCTAT

CGGCTATGT

CATGGGTGAA

CGATATGCCA

AGACGATGGG

TACCAGACG

AGTACCAAAM

GGGAANCNC I

TTACTGGANC

TGGGGGCAAC

GCGGGTCAC1 7TACACAAGG; TrTGCCG5AAC

CAAAACCAA(

k. TGATGAGGA( r AACAGGTGC( r' GAAGAACAC

GGCTGGCATI

TT'cCMTI CAAACAACCG AAGAGTCACA GGCAACGGTC GAAACGGCAA GAATTGCTIC TCGAGCGATT

CCCTTTCT

GTCAATCTTT

ACCACTGTGA

AC AAATAAC C

AAGGAGTATC

TATCCAGTGC

AATGCGGAGA

CCTAAAAAGA

ACCTATTTTT

GGTCCTGTGT

GGCGGGGCGA

GAGGA1TT1A

AAGACGTATT

ACCAAAACGC

GAAGAAGCAG

GATGAGCAAG

AGTGACGGAG

GCAAGTCAAT

ACAATCGTAA

ATTTCGATTT

GAAGTGCGCC

GCGTATANACT

CCACGT=TC

ATGGCGGAAA(

AGAATCCAAG

AAAGCCTGGA

CAACGGIII

CGGAATTAGA

CG=rAALATA

ATGGAGATGT

ATTATTATT

TTCCTGCGCC

CT=rACCCA

TA'IIAAAGG

CTAGTTATGC

TGATGAAAAC

GAAACTTIGAT

CCACAGGGGA

TAAAGAAA]T

CTTATGGCCC

CACCAACTAC

GTGGCATCGA

TGACCCAAAA

;TTTTATGAA

;TTACTGTCT

.AATATTTAT

:ATTCGACCA

n'CGAACGT

TIAACCAAT

rACGGTAGCT

:GGCGGTCCA

kACAAAGCGG kGAAGGT1'AT kGAAGGCACC rTGGTACAAA

:;GTGACCTAT

CTATACGTTG

TALATCCCGCC

AATGACGACT

AGCCATCCCG

GCAAGAAGTG-

TGcGTATACA

AAACCCCGAC

AAGTGccACA

TTACTCAATG

TGGCACCATT

TGGCGCAAAA

TGAAAACTAT

AACCTATATC

AACGACCCCA

AGAAGCGATT

cG~cCACAATG

CAITAAALATC

TGTACAAGGA

CGGTGCAGGA

TGGGGCCCCA CGAAGACACT TATAA=rAT CGACCCCTGA

GAACATITTG

AATCAGCTAG

ATCTACCAAG

CCAGCAACAT

GACATGACCG

GTTGACATAA

GGCGAAGCGC

GGCGCACCCA

ACCAAAGAAC

TAGACGAAAG

TGGTGGACAG

CTGGTGAAA-A

TAAAGACGAC

CTGTGTACCA

TCGAAAATG

CGTTAACCAC

ACACGATAT]T

AATGGACGAC

208 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

GTGTCCATCA

GGAACCGCCG

AACTITMGCA

ACGAGTCCTG

ATTCAGGAA

ACTCGCAACZ

CTATCGCAAC

ACGGCCGCTG

GGCAAGACCA

CAACAGTTCA

GA.AGTGCCAG

CGTTGGATCA

TTACAGGAAA

GT=AACTGC

ACGGCGACGG

GTAAGCAACA

CTTATTTACG

CAAAATCAGC

CTGCCAGC T

GCACCGTGAC

CAGGCAGTGA

CCAACCAAGG

GCCTTTACCA

TCCTGGTCAA

CAAAGATACG

CTTTATTAGT

ATATGGTTI'G

CCTGAATACT

CACAGACAGC

TACCGATTAC

TTAACCGCC

CGTCTATCAG

TATGGCTGGC

GCTGGCGGTC

GTTAACCG

GTCCGTA ETA

ACCCCGACAT

AAGAAGGCCG

AGCCAAGCCA

TTGCCAACAA

AACCAACCAA

GACAATACCG

TTGGACTTCA

.CAACAATACC

AATTAAAAAT

CTGATGTTGA

AAGACTTAGA

TTGAT'ITT"IGG

CAGATTACTA

ATTGGAGT

CGACCCCCTT

CAGAAACCAG

CAACAGCGGT

ccT'ITGCTAA

AAGCCGCCGT

GAACTTATTA

AGTAACGGGC

TCAAGAAATT

TAAACTAGCA

CGGCAATGGC

AACGGCCCAG

GTT-GCTAGGA

GACACCACTT

GGTCGCAAAC

CATCAAACTA

CACGTGGAAT

TTAGTGACTG GCCCCT EF107-4 (SEQ ID NO:416) a EQG'IATSQ SSDEASQTTQ

TTEESQATVA

SEAKTVPPQE TARIASRAIG YSSVEGREIP KLQRTPLEPT TVNGGKLLSI PTSPAFKYDT TFYTGDTTQY PVP IVFANVG GKVTNYVGAN APGGVTYALT YFYGDVGGPT SSHQRRGTAG QDKKTIVTGE DFTFTQEGTL

PERYTGSDGK

DNODLHVVYE EAVMKTYTLP AREALFGYVD PTIDGIDMPA SQLKKLAIPQ KVYTRPDOGT DKTKYPVPNE VRRGIENPDN

IVSSLVGXXA

IYSGTAGGNY NLSTPDGTIY

YYLENRRVTE

YTVAKALPKI YQAGEKTYIF

QGWFKGKTKP

TAELTLTGAV DIIENGATMD

YWEALLKNTG

C-QNTKAFPVT KEQWTTGAGV SITLDQPLPA FGSLTAXDT'J RIICDLDQEIT

SPDGDGFIST

RNPYLRLNTS QANWSLTAQL SQPKSATDSL KTSTVTLTAD NTATAVVANQ

QFTGSDVYQL

VTGP

FFM~EDGTL

NNQNPSNIYG

AETE.LELTNE

PVYYYLTKRR

TYLFKGWYKG

EQGC1LINPAK

IVTI'GPQEVS

YNLTQKSATR

HFVDESGAKI

ATLKTTTTPS

EAPLTTIKI.K

GGQLKM1NLLG

PTFDFGKLAI

PT±TTRLLLGT

DFTFANIKLE

FDPDRITMAV

VSvEVSFTIPK

KMPNKLTFGP

VTTEKFENPAG

NAKPSTLETT

FXLSATMGES

VEIPKYfYQTI

YTARRSYWXW

TPPTGFTQGN

FTPTFNDEDD

PTATWAAGIG

TAVTGNPGQV

SGSKQQYGLK

AAAASFTDYN

VPANQGMAGQ

NLSTFSFYEE

EYQSLDIRPS

KKTFKYTVAT

GA IPAP EGYT

KTPSYAVTYD

DGATGEMTTF

SI SPTTAYTG

GPTKTLYSMS

QLVVDSENYV

MTAVYQEAIP

APNTI FVQGT

L.TADVEVTGN

KAADYYGNGT

QPTETRTPLG

QYQAAvTWNL EF1O8-1 (SEQ ID NO:417)

TAATCGG=T

CAACGATTAG

ACGGCAGTTG

GAAGAGACGC

CAAGTAACAA

CAAGCAGAAG

AAGGCAACTT

CAATTAATAA

ACAGATGCTT

AACTTAAAGA

GAAGGAACTC

GCGAGTCTCT

CTAGCAGGTA

ACCAGTAAAG

CGTGTAAAGT

ATAGGTTATA

GAAAACTTCG

AAACAAACGG

ACCTACAAAG

GGCGGGAATC

CAACCATTGG

CGGAAACCGT

AAGCAAGTAG

CTGACACGAG

TGGAACAAGC

ATTCATTTTC

ATCCAGATAT

ATAATCGAAA

ATGTAATALAT

AAGAGGTGTA

ACAATACAGA

CTAGACCGCT

GAGACAATGT

TTACTAATT

AGACTGTGAG

TAGATACAAG

TCATTAACAG

TTGGCACGAA

:;TACATAGAA AGAAGGGACG. ACATGAAGCA

AACTAAGTGG

CTTGTGTAGT

GACGATTGAA

CGTGAAGGAA

TCAGGAAGAA

AGAAACACCA

TGCAGAAACT

TATACCAATT

GTGGACTAGT

GCcAGcAcT

CACAAACTTT

AGGTAAAATTI

ATCTrITGACT

TTATACACCA

'FrrGTATCGT

TAGCCCAATC

TGGCGCCAAA

TGATCCTTAC

ATCTTACCGA

rcrTAGTAA

AGTAGTCCGA

cAAACAACGA

GCAACGAAAG

ATCATTCCTA

TATCAGTTTG

ACGTATAGCT

ATGGTTCAAG

TCTGTAGTTA

TCAATTCGCA

GATTCTACTT

CAAAGTAGTG

CGGGAAACAT

ATAAATGACT

TATrACCATC

ATCACGCCAC

ACG~rCCAAC

TTCAAAGGC'I

CCGCCGAAAG

AAGCCAGTAC

A.AGCGGAGAA

AACCAA.AAAA

CATTTGTGAA

ATGCCAAAGG

GGAGTGCTTC

TOCCACCACO

TACCAAAATA

ATCCGTTACC

TAATTAGTGC

TTTTTrGGAGG

TTGATGTGA

TGACCAACCG

CAAGTAATTT

AAAGTGGTTT

GGTACAAAGG

TAGTGCCAAG

GGAAAATAGT

A.GAAGAACCG

AATCAATATG

TCAATCAGGT

ATCATGGAAG

A.ACCGTAGGA

ACCCTCATAT

TTATCCATCA

TGCTAT1'GCA

ATTGGCGCTG

AGATCCTGCA

AGGTAATAAC

CCGTGTCACC

CACCCAAGGG

TTTACCCGAG

GAAA.ACCAAA

209 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

et t 4

ACCGAGCCTT

GATTTGACGG

TTTGGCTTTG

TATAATCTTA

ACAGATACAG

GTCAATACGT

ATCAATATTA

AATATAGATC

CTGTCGGGCC

ACGTTATII'G

CCTGITTACT

GCTAAAATCA

GCCTACACTT

TACAAGTTCA

GCGCCAAGTT

GAAACAGTTA

GCTTTCACAC

TTA.AGAACCG

AGTATTAATA

CAACCAATCA

CAACTAAAA'I

TATAGATATC

GAGGTTGACC

TTTTCTAATC

GTTAACTATC

GTCTACAAAC

ATCACTGCAC

AAGTACACAC

TTCCAAGGG'

ACGTTCAATC

CCAACAGCT)

ATCTGGACA

AAAGGGCCCA

GGAGAAACG,

CCAAATGCCI

AA.ACCAAAC

GTGGATAAC

GGCTTCATC

CAACACAGC

CGGATTAAG

GCGACAGAC

TI'ACCAAIl

AGCTTAACP.

AATGTTTA'I

GGTGTTAAP

TGGCCACCAC

TGGTCTATGA

TGG.ATGAAGC

CITTAAATGA

CGACACTGAA

TTTATGGCGC

ccAAATCAGA

AAGTAGAAAT

AAACGTrGG

GGACAGAGTC

ATTATTAGA

CACCGCCAAC

TCA.AACAAGC

AAGGTTGGTA

ATCAAGTGAC

CGACAGTGTA

CGGCGTTAAC

ATTTATATGP

ATGGTAGTAM

GTGCTACCA

ATGTTGACAC

TGTACACGAI

TAGTTCAGZ

CAAATAATAC

rTTTCGTA.W.

TCACTCGCAj CAACAGGCr CGGCAkAAG C' CGTATAAAGC

CGACCTTIG.

'GTGTCACAT

D, CAACGATCA k. ATTGGTCAG k. CAAAATCAA TTcCTATCG A. CTGTTTTGA T TCGTGAGAA A GTGTGCCAP T TCAAACAAC A AAACGCAAC A GCTTGCCTI 'r ACAATCAA-C ,G CCAACAACJ IC AGTTGGAC' LG GGCAACAA'

TAAAACACCT

GGAGI'TTCA

GACGAACAAA

AAATAATAAA

AAACTTGTCC

GAGTGACATT

TGGCAAAACC

GTCACACATG

CTTTAATGCT

TGGCATCGAT

AAACCGCAAA

AGGTTTCACC

AGGCACCTTA

CAAAGGCAAG

CTACGATGAC

*TCCATCAGTC

*TTTTAGTGG'I

CGTGACCTC.P

GCCATTGTCC

CAGATTACAC

CATTCAATT;

CAATAGCTCI

kATCTC'ITAA(

;AC=TTTA(

r' cT'rrTTTT' k. ACAAGTCAC( r CACCCAAGG' :T'IT1ACCAGC( S GAAAACCAA, P. TGGCA-ATGA T AACTCGACC C GAATACTAG C TGGTCTGAC .T CCCAGTAAA .0 CAAAAAAGT A AGCAGAAGIl LT TCGTCCAAA.

,C CTTCGACTI ;C CGCGGA T; .C CAATTGGAC C AGCGACCCC :C AACCGAGT' kC AGCAACGA( Mr CACCTTICAj I'A CAAGGCCG(

AGCTATAAAG

GGGTACGAGC

TTAATTGCCC

AAAACAGTAA

GTGCC1XGTCA

ACGTTTACAT

GACCCAGCTT

CCTGTGACCA

TTAGCCGATC

GACCCAGTCA

GT'CACCGAGA

CAG GTAAAA

CCAGACACTT

TCCATACTCA

AATGATGATT

GATATGAACT

AAGTACTATG

AAAAATAATC-

CAAGAIrA'I

TTTAATGTT

GACACAGCTC

CTGGTTITCC

TTGC IAAT J

ACGCATTTA(

P' AACGCCAAA(

:GAAAACTTC(

r AAccAAGTA(

ACGTATACT,

SCCAAGTACG'

C GATATGACCI A AAAGAAGTG.

c AAAGCACCC G ATCCCGACC TCACGTATGA T TGCCTGCTTC

G

CCGACCAAGT

C

TGAGCAGTAA C ACTATTTTGA

A

TGCCCAAACG

C

TTCCTCTTCC

'I

CTTATAACA-A

C

AACCTGAATT

IJ

A'ITATI'ATAC

IZ

ACTTCGTAGA

AAACGGTGAT

ACACAACAGG

ACACATTGAC

TGAATGTGGT

*TTGTGAATGA

CGCAA.AGTAC

GTAATGGGCA

*TGAAAAAATA

ATAAATTAC

*AAAGTAGCAA

ACCCAAATGT

7 TTGATTCAGA

GATATAGTGG

-CTGCGGATAA

TGGATCTCAA

CAATGAACAG

k CAGGTGGCA.A r TGAACAAAAC

GCCATGTATAA

A TI'ATACGAA T TACAAAATCT T TTATGGA.AGT

'ACAATCAT

~CCAATCAA

AGATGAAG

rTAACGGG

CAATATCGC

TACAAATCA

~AAATCTAT

TTGAAACAG

rATACGAAA

ATGAGTGGC

PCCAACGGC

ACAAGCGAC

GGTAAGACC

LACTACCAAA

TATGAAGA-A

LAAAGGCGGG

,AGTGCGTAC

~TATACGGTA

~AATAATGGA

:ATCGACCAA

rcTGAAATcc kGCACCAGCA rCGGCACCTAT

CACACCAGGA

GTCAAAACTT

CGTCCCAAA

TAACACCTTC

AGTCTATACG

P.ACAACTCCA

GGAAGAAATA

TACCAATGTA

CACCTTGAA.A

GACACCAGAA

GGTTCCTITA

CGCAACAGGG

AGATACTACA

AACGACCGAA

AACTAAGCAA

TCCGTATCTG

ACcAAAATCA

TGT-CTCTAGC

CAGTGCCATT

CACAGGTAGT

AGCCA.ATCAA

ACGTCCTTAA

AGTACACTT GGACAGAGGG

GTAGTATTTG

GATCAAGAAG

GGCCAAGTGG

TACGGTAACG

TTAACAGCGC

TTATTA'rTAG

AAAAATACGG

ATTATTGCCA

AATGTCAAAC

GTTACATGGA

GT'GGTATI'AA

TAGTCACACC

GCGTTGCAGG

GCACACGGAA

AACTGTCACA

GGGCGGCGCC

TCCGTACCAC

ACAAGCAATI'

TTGAAGTGCC

ACCTAGTTAC

EF108-2 (SEQ ID NO:418) MKQTKWQ RLATIGLCSS LVINAFSGVT AVAETVTIES

SPTAESSAKE

ETQASSVKEE TrKASTENSQ VITDSQEE.A TKEAEKEEPQ

AEVEQAETPI

ATYSFSAETY QFGFVNESGQ LINPDI IP IT YSYAI(CSWKT

DGYNRKWTSM

LKNVIMPATS VVMPPGPSYE GTQEVYTNFS IRIPKYYASA

SLYNREGKID

ACTRPLSLTQ SSVISALALT SKGDNVYTPR ETFFGGDPAG

VKFTNFLYRI

GYKTVSSPIY YHLTNRRVTE NFVDTSCAKI TPPSNFTQCI(

QTVINSDPYT

IPKPKKINMK(

VQGSASTVGN

STYPLPAIAL

NDFDVKGNNI

FQQSGFLPET

210 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

YKVGTKSYRF

GFVDEATNKL

NTFYGASDIT

SGQTFGFNAL

KITPPTGFTQ

PSYQVTYDDN

RTD)LYDVTSK

LKYVDS IQLD

SNANNRLFYT

TAPTGFTQGN

FNATFDGNDD

SP NWSAGLT I

PNTVI.AAEVV

HSICQA.ADYY

TNYNQPTELK

VKGQQYKAAV

KGWYKGKTKT

IAPOQVQMXY

FTLPKRY(S I

ADQPEFYTKT

GI{KTVITSDA

DDLNVVYEEE

NNGNGQYTVS

TAQSSNLKSY

HLGYSGTPGV

QVPMNSNTFK

MTAMYKEEIP

PTFMLVTPEG

VrGG IKDSTV

GNGTRNPYLR

NTVGTTSAIS

TWNLVTGP

EPLATTKTPS

NLTLNENNKK

NIITKSDGKTD

LFGTESGIDD

YTFKQAGTLP

TVTTVYPSVD

INNGSMPLSQ

RYVYTNNSSL

NYLLVMFLFN

YTAAKALPAT

TAS VTLTRPK E'FKS IPVNS

DNFVRIRPND

IKKTQPNWSL

LTANNTATS I YKVTIYCDNDD LTvvYEEFSG YELPASTNQF TVMSSNLTGT.DTATLK<NLSV

PVNYFEQYRV

PAFPLPXIYN

PVNYYTMSGP

DTYTTGGKTY

MN4FVNEKGGA

ELLKKYNNGQ

VFDPNVAPAE

AKPADKSKLV

YTTGGKVYTF

EVIDTNTNVI

TLW tEVPLP

QEVVTPTTEG

TAQLSQPKSA

IANKQFT S N I DQVM-4SHMP

VYYYLENRKV

KFKGWYKGKS

FTPALTFSGK

PISATNRLQF

VDLSSESLNL

YKVTRXKQVTE

QGWYKGKTKP

W=rITNTSI( NAVP IGKQCVS

FISVPTFDFG

TIDSLP.TAThL

VYQLDFT-.M

TEN~FVDTNGA

ILNTL LTTKA

YYAQSTSAYL

NVOKLAIDQQ

LNFOSOGTYF

NFVDVNGAK I

STLNKTTTPT

APLQNLTLKK

VAFTTRATGK

QVGVAGTKQQ

LLGAAPVSSF

VKLEVPANQG

EF108-3 (SEQ ID NO:419)

V.

V

CGT GACGATTGAA AGTAGTCCGA CCGCCGAA TAGTGCC

GAAGAGACGC

CAAGTA-ACAA

CAAGCAGAAG

AAGGCAACTT

CAATTAhATAA

ACAGATGGTT

AACTTAAAGA

GAAGGAACTC

GCGAGTCTCT

CTAGCAGGTA

ACCAGTAAAG

GGTGTAAAGT

ATAGGTTATA

GAAAACTI'CG

AAACAAACGG

ACCTACAAAG

ACCGAGCCTT

GATTTGACG

TT1'GGCTTTG

TATAATCTA

ACAGATACAG

GTCAATACGT

ATCAATATTA

AATATAGATC

CTGTCGGGCC

ACGTTATTTG

CCTGTTrACI

GCTAAAATC.A

GCCTACACTI

TACAAGTTCP

GCGCCAAG79

GAAACAGTT;

GCTTTCACAC

AAGCAAGTAG

CTGACACGAG

TGGAACAAGC

ATTCATT'-TC

ATCCAGATAT

ATAATCGAAA

ATGTAATAAT

AAGAGGTGTA

ACAATAGAGA

CTAGACCGCT

GAGACAATGT

TACTAATT

AGACTGTGAG

TAGATACAAG

TCATTAACAG

TTGGCACGAA

TGGCCACCAC

TGGTCTATGA

TGGATGAAGC

CTI'TAAATGA

CGACACTGAA

T[TATGGCGC

CCA.AATCAGAP

AAGTAGAA.Al

AAACGTTTGC

GGACAGAGTC

ATTATTTAGI

CACCGCCAAC

TCAAACAAGC

AAGGTTGGT2

ATCAAGTGA(

CGACAGTGTj

CGGCC'ITAA(

CGTGAAGGAA

TCAGGAAGAA

AGAAACACCA

TGCAGAAACT

TATACCAAT'

GTGGACTAGT

GCCAGCAACT

CACAAACTTT

AGGTAAAATT

ATCTTTGACT

'ITATACACCA

TTTGTATCGT

TAGCCCAATC

TrGGCGCCAAA

TGATCCTTAC

ATCTTACCGA

TAAAACACCI

GGAGTI'TTCP

GACGAACAA.P

*AAATAATAAJ

*AAACTMTCC

GAGTGACAT]

TGGCAAAAC(

GTCACACATC

cITTAATC

TGGCATCGA'

AAACCGCA-k

AGGT'ITCAC(

AGGCACCTT,

k. CAAAGGCAAI

-CTACGATGA,

k. TCCATCAGT'

:TTTTAGTGG

GAAACAACGA

GCAACGAAAG

ATCA'ITCCTA

TATCAGTTTG

ACGTATAGCT

ATGGITCAAG

TCTGTAGTTA

TCAATTCGCA

GATTCTACTT

CA AAGTAGTG

CGGGAAACAT

ATAAATGACT

TATTACCATC

ATCACGCCAC

ACGTTCCAAC

*TTCAAAGGCT

*AGCTATAAAG

GGGTACGAGC

L TAArrrGCCC

LAAAACAGTAA

GTGCCTGTCP

ACGTTTACA'I

GACCCAGCT']

CCTGTGACC;

r TTAGCCGrATC r' GACCCAGTCI

GTCACCGAGI

:CAAGGTAAPJ

k~ CCAGACACT'J 3TCCATACTCj

AATGATGAI

C GATATGAAC' T AAGTACTAT A AAAAATAATI

:AAG

AAGCCAGTAC G AAGCGGAGAA A

AACCAAAAAAA

GATTTGTGAA

ATGCCAAAGG A~

GGAGTGCTTC

TGCCACCAGG TACCAAAATA 9) ATCCGTTACC 9

TA-ATTAGTGC

TTTTGGAGG

TI'ATGTGAA

TGACCAACCG

CAAGTAATTT

AAAGTGGTTI!

GGTACAAAGG

TCACGTATGA

TGCCTGCTTC

CCGACCAAGT

TGAGCAGTAA

LACTATTTTGA

TGCCCAAACG

TTCCTCTTCC

CTTATAACAA

AACCTGAATT

k ATTATI'ATAC k. ACITCGTAGA k AAACGGTGAT r' ACACAACAG

ACACAI'GAC

r TGA.ATGTGGT r TTGTGAATGA 3 CGCAAAGTAC 3 GTAATGGGCA

,GAAAATAGT

.GA.AGAACCG

,ATCAATATG

,GAATCAGGT

LTCATGGAAG

ACCGTAGGA

~CCGTCATAT

'TATGCATCA

EGCTATTGCA

=TIGGCGCTG

k.GATCCTGCA

%.GGTAATAAC

CCGTGTCACC

CACCCAAGGG

rTTACCCGAG

GAAAACCAAA

TGACAATGAT

GACCAATCAA

GCAGATGA-AG

CTTAACGGGG

ACAATATCGC

GTACAAATCA

TAAAATCTAT

GT1'GAAACAG

TTATACGAAA

AATGAGTGGC

CACCAACGGC

TACAAGCGAC

CGGTAAGACC

AACTACCAAA

GTATGAAGAA

AAAAGGCGGG

GAGTGCGTAC

ATATACGGTA

'AAGAACCG ATI'TATATGA CGTGACCTC TABLE 1. Nucleotidz and Amino Acid Seqeuences of E. faecalis Genes.

AGTATTAATA

CAACCAATCA

CAACTAAAAT

TATAGATATG

GAGGTTGACC

TTTTCTAATG

GTITAACTATC

GTCTACAAAG

ATCACTGCAC

AAGTACACAG

TTCCAAGGGT

ACGTTCAATG

CCAACAGCTA

ATCTGGACAA

AAAGGGCCCA

GGAGAAACGA

CCAAATGCCG

AAACCA.AACA

GTGGATAACT

GGCTTCATCA

CAACACAGCT

CGGATTAAGA

***GCGACAGACA

'rTTACCAATT

:AGCTI'AACAG

AATGTI'TATC

GGTGTTAAAG.

kTGGTAGTAT ,TGCtACCAA ATG TMACAG

TGTACACGAA

rTAGTTCAGA cAAATAATAG

TTCTCGTAAT

TCACTCGCAA

CAACAGGCTT

CGGCAAAAC

GGTATAAAGG

CGACCTTTGA

GTGTCACATT

CAACGATCAC

ATTGGTCAGC

cAAAATCAAT

TTCCTATCGG

CTGrT=GAA

TCGTGAGAAT

GTGTGCCAAC

TGAAACAAGC

AAACGCAACC

GCTTGCCTAC

ACAATCAACC

CCAACAACAC

AGTTGGACTI

GGCAACAAT;

GCCATTGTCC CA.AGAATTAT CAGATTACAG

TTTAATGTTG

CATTCAATTA GACACAGCTC CAATAGCTCA CTGG=FTCG ATCTCTTAAC TTGCTTAATT ACTTTTTTAC ACGCKPI'TAG GTTCTTTTI' AACGCCAAAC ACAAGTCACC GAAAACTTCG CACCCAAGGT AACCAAGTAC TTACCAGCG ACGTATACTA

GAAAACCAAG-CCAAGTACGT

TGGCAATGAC GAtATGACCG AACTC--ACCA AAAGAACTGA GAATACTAGC AAAGCACCCT TGGTCTGACG ATCCCGACCT CCCAGTAAAT AGTACACTTT CAAAAAAGTT TCAGTTCT AGCAGAAGTT

GTAGTATTTG

TCOTCCAAAT GATCAAGAAG C'rTCGACTTC GGCCAAGTG CGCGGATTAC

TACGGTAACG

CAATTGGAGC TTAACAGCGC ACGACCCGC TTATTATTAC AACCGAGTTG

AAAAATACGC

AGCAACGAGT ATTATTGCCI CACCTTCAAT AATGTCAAAc CAAGGCCGCA GTTACATGGI TGAAA AAATA TAATAP.TGGA ATAAATTAGC C AAAGTAGCAA I1 ACCCAAATGT TTGATTCAGA I

GATATAGTGG

CTGC--GATAA

TGGATGTCAA

CAATGAACAG

CAGGTGGCAA

TGAACAAAAC

CCATGTATAA

TTGA1IACGAA

TACAAAATCT

TI'ATGGAAGT

GGACAGAGGG

TCACAACTCG

GTGGTATTA-A

TAGTCACACC

GCGTTGC-AGG

GCACACGGAA

AACTGTCACA

GGGCGGCGCC

TCGGTACCAC

ACAAGCAATT

TTGPLAGTGCC

SACCTAGTTAC

:ATCGACCAA

~CTGAAATCC

LGCACCAGCA

LGGCACCTAT

ACACCAGGA

'TCAAAACTT

:GGTGCCAAA

rA.ACACCTTC

AGTCTATACG

AACAACTCCA

GGAAGAAATA

TAC.CAATGTA

CACCTTGAAA

GACACCAGAA

GGTTCCTTTA

CGCiLACAGGG

AGATAGTACA

AACGACCGAA

AACTAAGCAA

TCCGTATCTG

ACCAAAATCA

TGTCTCTAGC

GAGTGCCATT

CACAGGTAGT

AGccAATCAA

AG

EF1OS-4 (SEQ 1D NO1:420) VTIES SPTAESSAKE a

ETQASSVKEE

ATYSFSAETY

LKNVIMPATS

AGTRPLSLTQ

GYKTVSSP IY

YKVGTKSYRF

GFVDJEATNKL

NTFYGASDIT

SGQTFGFNAL

K ITPPTGFTQ

PSYQVTYDDN

RTDLYDVTSK

LKYVDS IQLD

SNANNRLFYT

TAPTGFTQGN

FNATFDGNDD

GPNWSAGLTI

PN'IVLKAEVV

HSLKQAADYY

TNYNQPTELK

TTKASTENSQ

QFGFVNESGQ

VVMPPGPSYE

SSVISALALT

YHLTNRRVTE

KGWYKGKTKT

IAPDQVQMXY

FTLPKRYKS I

ADQPEFYTKT

GK'KTVITSDA

DDLNVVY'EEE

NNGNGQYTVS

TAQSSNLKSY

HLGYSGTPGV

QVPMNSNTFK

MTAMYKEE IP

PTFMEVTPEO

V'FGG IKDSTV

GNGTRNPYLR

NTVCTTSAIS

V'ITDTSQEEA

LINPDI IPIT

GTQEVYTNFS

SKGDNvYTPR

N~FVDTSGA.KI

EPLATTKTPS

NLTLNENNKK

NITKSDGKTD

LFGTESGIDD

YTFKQAGTLP

TVTTVYPSVD

INNGSMPLSQ

RYVYTNINSSL

NYLLvMFLFN

YTAAYKALPAT

TASVTLTRPK

ETTKSIPVNS

DNFVRIRPND

IKKTQPNWSL

LTANNTATSI

TKEAEKEEPQ

YSYAXGSWKT

IRIPKYYASA

ETFFGGDPAG

TPPSUFTQGK

YKVTYDDNDD

TV14SSNLTGT

PAFPLPKIYN

PVNYYTMSGP

DTY'ITGGKTY

MNFVNEKGGA

ELLKKYNNGQ

VFDP.NVAPAE

AKPADKSKLV

YTTGGKVYTF

EVIDTNTNVI

TLWTEGVPLP

QEWVTPTTEG

TAQLSQPKSA

IANKQFTGSN

AEVEQAETPI

DGYt4RKWTSM

SLYNREGKID

VKFTNFLYRI

Q IVINSDPYT

LTVVYEEFSG

DTATLI<NLSV

IDQVEMSHMP

VYYYLENRKV

KFKGWoYKGKS

FTPALTFSGK

PISATNRLQF

VDLSSESLNL

YKVTRKQVTE

QGWYKGKTKP

WTTTITNTSK

NAVP IGKKVS

FISVPTFDFC

TDSLPTATRL

VYQLDFTFNN

IPKPKKINmK

VQGSASTVGN

STYPLPAIAL

NDFDXTKGNNI

FQQSGFLPET

YELPASTNQF

PVNYFEQYRV

VTTYNKLKQL

TENFVDTNGA

ILNTLTI'rKA

YYAQSTSAYL

NVDKLAID:QQ

LNFDSDGTYF

NFVDVNGAKI

STLNI(TTTPT

APLQNLTLKK

VAFTTP-ATGK

QVGVAGTKQQ

LLGAAPVSSF

VKLEVPANQG

VKGQQYKAAV TWWLVT EF109-1 (SEQ ID NO:421) 212 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

AGGAGTAAAT

GGAITTCTCG

GAAACGGTGG

GAAATGAACC

ACCTCCGATT

NATAGTAGCG

TAG

TAATGAAAAA

TTACCCCTAT

CTTCAGAAAC

CAAGCATCAT

CCACCACTGA

ACGTACTGAA

AAGTGTTATA

TTCTGCTTAC

ATCTCTA-ACG

kAATTCTCAA

AGTTTCTACA

ACTACTITTGG

ACTAGTTCTA

GCTITGGAAC

GAGCGACAAA

GAGGAAACAG

TCAGAAGTAA

NAACATCACN

TGT -AGCGGT

GCTCTAAGGG

TGAGTAGCGG

AAACAACGTC

CAACTGTI'AA

AAGTAATGAG

TTTGTGTCG

AACTACTGAA

TGTCACTGAA

CACTTCCTCA

TGATACAGAA

GACACACCTA

EF1O9-2 (SEQ ID NO:422) MKUKSVI TSSMLAVLLS GFLVTPISAY ALERSKGTTE ETVASETSLT ERQMSSGVTE EMNPS IINSQ EETETTSTSS TSDSTTEVST SEVTTVNDTE XSSDVLKLLW XHHXVMRTHL EF109-3 (SEQ ID NO:423) GGAAC GCTCTAAGGG AACTACTGAA GAAACGGTGG CTTCAGAAAC ATCTCTAACG GAGCGACAAA TGAGTAGCGG TGTCACTGAA GAAATGAACC CAAGCATCAT AAATTCTCAA GAGGAAACAG AA.ACAACGTC CACTTCCTCA ACCTCCGATT CCACCACTGA AGTTTCTACA TCAG EF1O9--4 (SEQ ID NO:424) ERSKGTTE ETVASETSLT ERQMSSGVTE EMNPSIINSQ EETETSTSS TSDSTTEVST S EF110-1 (SEQ ID N1O:425) S S

S

S

TAAATAAAA

AGTGCTGGTT

AATGCAGAAG

GACCCTGAGG

GGAAGAATCA

GTTGGAACCA

GTATTAAATC

ACACCAT'ITG

GTAGTGACTG

ACGCCAT'ITG

CCAGGT'GAGA

ACAGACTTAG

TCACCAATCT

CAAACAGGAA

GTGAATGAAG

TrGGATAAGGA Tr ICTTTCT

AGTATATCGT

ACAGAAGACA

TTTCCCCTGC

ATACAATIGT

CGAATGCCAA

GCAAAITCAA

TCGGCAAACA

TTTTGAAAAA

AA-AACCACAC

AAAATCCT

ATAATGATCA

ATCATGGTCA

AAGAAAATAA

GTGGCATAAT

GATTTTAGTA

TCCTGCCGAA

AGAAGTGGCA

CAGTAAACCA

CACCAATAAT

AGATGATGCT

AGTGATTGAT

AAACGATCGT

GTrTGAATCT

ACAATGGTCT

ACTATTAT

GGTI'GAAGTA

AAGACTAAAT

ACGTTTATCC

CTTATGAAAA

ACTI'TGATCG

AdTCATTCAC

GATACAACCG

GGCTATATTT

CATGTGGCTG

TGGTTTTATC

GTAGCTTTTT

CCAGATGGCC

TCAGATACCC

CATGAAAATG

GATATCGATA

GTTGGTGTTC

GAAGTGAATT

GCTGTGCCAG

AG'I'1CTZCAT

CCGGTITTAG

GACAAAAA.AG

AAGCGCCTTT

CT'ITAGGAAC

AAAGTTTTAA

CAGGTCGAGA

CCCCGAATGC

CAGAGTTGGG

ATGTCACAAT

ATFTTTTTAC

CAACCGGCGG

ATTCCAATGG

ATAACTTTAT

CAGCGTAA

ACGAAAAATT

CITGTCTGCA

ATCGTTACTG

TGCGTCAATC

AGGC ITTGTT

GAATGCCAAA

TGGCAGTGCG

GGATATTGCG

AGAAATTTTA

ATCAGGCTAT

ATCTAACTTT

TCAATC~iGT

CGGCATTAAG

TGTTAATCGA

EFI1O-2 (SEQ ID NO:426)

MKKFSIRKIS

PEDRRQEVAD

LNPNAKDDAW

PFVLKKFESS

P IYNDQVEVV AGFLFL ILVT

TTEAPFASIG

FYPGRDGSAT

DTHVTISGYP

GVIISNGGIKQ

L IAGFSLSAN

RIISPASKPG

PFGKFKVIDV

GEKNHTQWSH

TGNHGQRLNE

AEEYIVPAES

YISLGTGFVV

AFSPNADIAV

ENDLFTSNFT

vNYNFIVNRV

HSRQKRSLLD

GTNTIVTNNH

VTVGKQNDRP

DLENPLLFYD

NEEENKRLSA

VAESFKNAKI

DGPELGEILjT

IDTTGGQSGS

VPAA

EF110-3 (SEQ ID NO:427) AG AGTATATCGT TCCTGCCGAA AGTCATI'CAC GACAAAAAAG ATCGTTACTG 213 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

GACCCTGAGG

GGAAGAATCA

GTTGGAACCA

GTATTAAATC

ACACCATTTG

GTAGTGACTG

ACGCCATTTG

CCAGGTGAGA

ACAGACTTAG

TCACCAATCT

CAA.ACAGGAA

GTGAATGAAG

ACAGAAGACA

TTTC CCC TG C

ATACAATTGT

CGAATGCCAA

GCAAATTCAA

TCGGCAAACA

TTTTGAAAAA

AAAACCACAC

AAAATCCATI'

ATAATGATCA

ATCATGGTCA

AAGAAAATAA

AGAAGTGGCA

CAGTAAACCA

CACCAATAAT

AGATGATGCT

AGTGATTGAT

AAACGATCGT

GTTTGAATCT

ACAATGGTCT

ACTA'ITI'AT

GGTTGAAGTA

AAGACTAAAT

ACGTTTATCC

GATACAACCG

GGCTATATIT

CATCTGGCTG

TGGTTTTATC

GTAGCTTTTT

CCAGATGGCC

TCAGATACCC

cATGAAAATG

GATATCGATA

GTTGGTGT'rC GAAGTGAA 1T GC(TtCCAG

AAGCSCCTTT

CTTTrAGGAAC

AAAGTTTTAA

CAGGTCGAGA

CCCCGAATGC

CAGAGTTG-GG

ATGTCACAAT

ATTTGTTTAC

CAACCGGCGG

ATTCCAATGG

ATAACTTTAT

CAGCGT

TGCGTCAATC

AGGCTTTGTT

GAATGCCAA-A

TGGCAGTGCG

GGATATTGCG

AGAAATTTTA

ATCAGGCTAT

ATCTAACTT'T

TCAATCTGGT

CGGCATTAAG

TGTTAATCGA

EF110-4 (SEQ ID NO:428) EY IVPAES HSRQKRSLLD PEDRRQEVAD TTEAPFAS IG LNPNAKDDAW FYPGRDGSAT PFVLKKFESS DTHVTISGYP PIYNDQVEVV GVHSNGGIKQ RI ISPASKPG

PFGKFKVIDV

GEKNHTQWSH

TGNHGQRL.NE

YISLGTGFVV

AFSPNADIAV

ENDLFTSNFT

VNYNFIV']FV

GTNTIVTNNH

VTVGKQNDRP

DLENPLLFYD

NEEEN'KRLSA

VAESFKNAKV

DGPELGEILT

IDTTGGQSGS

VPAA

EF111-1 (SEQ ID NO:429) C.

C.

TGATCAATAC

AAAAAGGGAT

CTATCATTTT

TGGTTAGCGT

AGTGTTGAAA

ACCGAACGGA

GCATGGCATG

GGGCCATTGA

ACGGTCAAAA

CTGTCTTATT

'PTAGCTI'AA

GGTGAAGAC

GAGGGAACGG

GTGAAT=ITG

ACGATITCATC

GCTGAACCAA

ACTT'ITACAA

GCGGCGCGCT

GATCAAAAGA

ACGA TAATA

TCCATGTCTT

GCAACCCCTG

CAAATTCAAA

TTITACAATC

AAACCACCAI

TTITrAAAAC

GACCACAAT;

AAAGACGAC;

CTGTTATTCl

AAGGTGTGGC

TAG

ACTTCGATAC

ACACTCCATT

ATAGGAGGGG

TAGGAATCAC

GAACAAAAGT

CGAATGGCGT

GCTACTATTT

TTATTGCGGA.

ATAAAAAAAC

ATCCTGGGCG

TTTTTGTCAG

CCTTGTCACT

AAACCTTAGA

GTGAAGTCAG

ATGCGGATAA

TTGAATAA

AAGAAGAAGA

ATTTCAAAGA

AAACAGCAGA

CCAATTGGCG

ATAAATGGT'I

ATT ITAATCC

AAGATGATAC:

AAGACAGTGC

TCCCTGPATG

AAATGTATCC

AAAATGGGAI

AGGATCAAAJ

kAGCAGCCGCC

'CAAAGGCGA(

GGTCGCTTTT TTTCTAGAGA TGGCATAGTC CTrGCTGATA TTTTATGAAG GGTTTATCAA CGTAGTCAGC

TGTTIGCCT

TGATGAXFI'T GCAAATGTTT ATACGATACC AAI'ATTTTA2 ACCTCAAAAA AGCAATAAAG2 AGAATATCCA

GTAAACTTGG

GGGAGAA-ACC TATGATTTAA CCTAGAGCAL ACCTATGAAT CAATCGAACG GCGC ETATCC TGCAGCAAGC TGGACAGGTG TATTGGCACT CGTTI'AACTG AGAAACGTGG AATTATITG AGT'I CAACA AAAATTGATA GCCTAAACAA ACGTACAACA GGCAAAGGAA CAACAACAAG GAACAAGCAA AGAVGGCAAG ATTCCTCAA TATCAAAATG AAGTGCGGCA GGTGCCTTIA *TATTGGTATG TGGGCTTGGG

*TGAGTTAGCT.AAAAATAATA

CGTACGTCCA CAAGATGCAG GCGTGGTGGT GAAGGTGGCA GGCAGTT1'GG CATAITTTATC CAA.ACTTGTG GC LTATCATA AGCAGAATAT GGAAGCATGG CATTAAAGAT AAAAATGGCC TGGGAAAGTG GCATGGATAA GTGGAGTTA AAGTTTITA ~.GTTGAATC T ~TAAATCAGT G

LAAAGAAACGG

rAAGCAGGGA ['AGATGTGAG %TAATT'rITc T :LGCTACTGGG TI :GGCAAGTTT GCCAAAGCAA

C

rAGACGAI'T

AAACGACACT

CGGTCTIGAC

CTAAAGACAA

CTACGAAAGA

ATCGGAATTA

CCTATACGAC

CACCCGAATA

GATATCTAGA

CCCTACTCAA

AGCATGACGG

ATTCGTGGAA

TGCCGGCCTT

GAGCGATCAT

ACTGGAATGA

AAGAAACCAA

ATTGGTGGTA

TCAGTGATGC

AccTAAAGTG

CCCTACACGC

AAACAAAAAT

TTCAATAAT

TATAAAGCG

GTGTCTACT

GTGCAAGCA

IGCATCACCA

GATTTAGGT

GGTTTTGCG

LACAAATTA

:CCCATGC-AC

LACGAITCAT

rGAAAACACT

:AAAATTCAA

L'GACATTCA

CACAAAATAT

E'ACAGCAACC

AGAAAGCTAT

rACCAAAAAT

TAAAACGTTT

ATCGATTGAA

GATTGTTCCG

AGCGGATGTA

GTTTGATI'AT

TCATGCTGTC

ACGAALATTCT

AGATAAGGAA

TACCAACAGA

TCACTGGCAA

GATGATGATG

TTTGACAAAG

AAAGGAAAAG

214 TABLE 1. Nucleotide and Amino Acid Se.qeuences of E.f[aecalis Genes.

EF111-2 (SEQ ID NO:430) MK(C LSKKKRVSTW

LALGI'IVVSC

WEGYYLPEKS

SYYPGRLEQT

GTETLDIGTR

EP IELKXIQT

QKXTAEFPEY

TADFNPELAK

PP LAAWAVWH DDKDQ IIKDI(

FALSREVQAS

NKELLGGFAG

YELDOLTIEL

LTAKONDIQV

VNTY-TTF-SYT

QNALVKS lET

NNMRALFD)YQ

IYQETKDKEF

NGHLKWMMML

VERTKVDEFA

PLIIAEEYPV

ALIFVSNRTA

NFGEVRETWN

FTKEEEAKEQ

INTNWRSA-AG

IQKDDTVRPQ

LKEM~YPKLVA

LLKQPRGKVA

NVLDVSAS PT

NLAASLNKLT

LIQTTLENTG

YFATKDTKYT

QQAPEYTKNA

AFKHDGIVPS

DAGAI IDAVF YHI4IWYTNRD WI TLHGLTKK 7RTNGVYDTN

VKNKKTGETY

EEPLSLGASW

IHHADKVSTK

ARYFKENKQR

MSYKWFIGMW

YNQDSARGG-E

HNKNG IAEYG

VWAKATLELK

YFNNFSDLGA

DLSQSNRMDL

TGAVFDKIQE

IDNRNYTATA

WQGYLDKTFD

AWDSWI(ADVA

CGNWNERNSK

SMVSDAHWQK

FLKTKIKEK

EF111-3 (SEQ ID NO:431) a.

a a a a a a a.

E-ATGAATTIT

ACCGAACGGA

GCATGGCATG

GGGCCATTGA

ACGGTCAAAA

CTGTCTTATr

TTAGC'ITAA

GGTGAAGAGC

GAGGGAACGG

GTGAATTITG

ACGATTCATC

GCTGAACCAA

AC7TTACAA

GCGGCGCGCT

GATCAA~AAGA

ACGATTAATA

TCCATGTCTT

GCAACGGCTG

CAAATTCAAA

TTTI'ACAATC

AAACCACCA7 ?rTTITAAAAC

CACCACAATP

AAAGACGAC;

CTGTTATTG;

AAGGTGTGGC

GCAAATGTT

CGAATGGCGT

GCTACTATTT

'TATTGCGGA

ATAAAAAAAC

ATCCTGGGCG

TTrTTGTCAG

CCTTGTCACT

AAACC'ITAGA

GTGAAGTCAG

ATGCGGATAA

TTGAA ITGAA

AAGAAGAAGA

ATTCAAAGA

AAACAGCAGA

CCAATTGGCG

ATAA.ATGGTT

ATTTTAATCC

AAGATGATAC

AAGACAGTGC

TGGCTGCATG-

1AAATGTATCC

,AAAATGGGAI

LAGGATCAAA!

AGCAGCCGCC

CAAAGGCGAC

TAGATGTGAG

ATACGATACC

ACCTGAAAAA

AGAATATCCA

GGGAGAAACC

CCTAGAGCAA

cAATCGAACG

TGGAGCAAGC

TA'FTGGCACT

AGAAACCTGG

AGTTTCAACA

GCCTAAACAA

TGCATCACCA

AATTATTTTA

AGCAATAAAG

GTAAACTTGG

TATGATTTAA

ACCTATGAAT

GCGCTTATCC

TGGACAGGTG

CG~TAACTG

A.MTATTTTG

AAAATTGATA

ACGTACAACA

C

C

GGCAAAGGAA CAACAACAAG GAAcAAGCAA AGATGGCAAG ATTICCTGA-A TATCAAAATG AAGTGCGGCA GGTGCCTT1'A TATTGGTATG

TGGGCTTGGG

TGAGTTAGCT AAAAATA6ATA CGTACGTCCA

CALAGATGCAG

GCGTGGTGGT

GAAGGTGG-CA

GGCAGTTTGG

CATATTTATC

CAAACTTGTG GCTTATCATA AGCAGAATAT GGAAGCATGG CATTAAAGAT

AAAAATGGCC

TGGGAAAGTG GCATGGATAA GTTGGAGTTA

AAGTT

~TAATTT'ITC TI ~GCTACTGGG GCcAAGTTT ;CCAAAGCAA C DAGACGATTI k.AACCACACT

CGGTCITTGA

CTAAAGACAA

:TACGAAAGA

ATCGGA.ATI'A

CCTATACGAC

CACCCGAATA

GATATCTAGA

CGCTAGTCAA

AGCATGACGG

ATTCGTGGAA

TGCGGGCCTT

GAGCGATCAT

ACTGGAATGA

AAGAAACCAA

ATTGGTGGTA

TCAGTGATGC

ACCTAALAGTG

CGCTACACGG

GATTTAGGT

rGGTTI'TGCG LAACAAAT'rA

:CGCATGGAC

ACGATTCAT

CGAAA.ACACT

:AAA6ATTCAA 1'GACATTCAA

CACAAAATAT

TACAGCAACC

1GAAAGCTAT

TACCAAAAAT

TAAAACGTTT

ATCGATTGAA

GATTGTTCCG

AGCGGATGTA

GTTTIGATTAT

TGATGcTGTC

ACGAAATTCT

AGATAAGGAA

TACCAACAGA

TCACTGGCA.A

GATGATGATG

TTTGACAAAG

EF111-4 (SEQ ID NO:432) DEFA NVLDVSASPT ERTNGVYIDTN

YFNNFSDLGA

WHGYYLPEKS

SYYPGRLEQT

GTETLDIGTR

EP IELKPKQT

QKKTAEFPEY

TAOFNPELAK

PPLAAWAVWH

DDKDQI IKDK

NKELLGGFAG

YE-LDDLTIHL

LTAKDNDIQV

YNTYTTESYT

QNALVKSIET

NNMRALFDYQ

IYQETKDKEF

NGHLKWMMML

PLIIAEEYPV

ALIFVSNRTA

1NFGEVRETWN

FTKEEEA(EQ

INTNWRSAAG

IQKDDTVRPQ

LKEI4YPKLVA

LLKQPRGKVA

NLAASLNKLT

LIQTTJENTG

YFATKDTKYT

QQAPEYTKNA

AFKHDGIVPS

DAGAI IDAVF

YHNWWYTNRD

WITLHGLTKK

VKNKXTG-ETY

EEPLSLGASW

IHHADKVSTI(

ARYFKZENXQR

MSYKWFIGMW

YNQDSARGGE

HNKNGIAEYG

VWAKATLELK

DLSQSNRMDL

TGAVFDKIQE

IDNRNYTATA

WQGYLDKTFD

AWDSWKADVA

GGNWNERNSK

St4VSDAN

WQK

215 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

EF117-1 (SEQ ID NO:433)

TAATTCGA'

GCTTTAACAG

AAGGAAACGN

GAATATATfl'

AAAGGTGATC

AAGAAAAAAT

AAAGGGACAT

GAGAAGGTG

GTTGTAAATC

CACAGTCTTA

CTTTGAAAGG

GTTT'TATTTT

TGAAGATTGG

TAATTGAAAG

TTTAGTGAAA

CGGTGAAAAA

NTCAGAAACA

GGAGATTCGTT

GAAAAGTGGT

TGACGAAGTG

TGAGGAGAAT

AGATTTTCAT

GAATTTGATG

GAATTACAAA

GAGAGTGACA

TCTAGTAAAT

ACAGTTI'ACG

CATGATTCAG

'I =ITTTACT

AAGAATCTCT

ATGGTGACGT

GTCr-TTCCAG ATcAAGTTTN

GAGAATATTA

CCACGAATTA

AATTTTACTT

TCAAAATCTA

TCGTTTAAAT

TTTAATAAAA

TAATGAGCAA

CGGCTTTFITG

EF117-2 (SEQ ID NO0:434) VKR FSFFLLILLA LTGCKSGEKE FDEESLQNLK ETXQSXSETE

LQNGDVRLNE

YISLKGEIVE SDSRSSLIKK GDR-FILKSGS SKYQVXNEQK KKLKIGDEVT

VYGEYY'GFLK

GTLIESEENH

DSATN

EF117-3 (SEQ ID NO:435) TG AAGAATCTCT

TCAAAATCTA

AAGGAAACGN CACAGTCTTA

NTCAGAAACA

GAATATATTT CTTTGAAAGG

GGAGATTGTT

AAAGGTGATC GTTTTATT

GAAAAGTGC-T

AAGAAAAAAT TGAAGATI'GG

TGACGAAGTG

AAACCGACAT TAATTGAA.AG

TGAGGAGAAT

GAATTACAAA

GAGAGTGACA

TCTAGTAAAT

ACAGTTTACG

CATGAT'rCAG

ATGGTGACGT

GTCGTTCCAG

ATCAAG7MT

GAGAATATTA

CCACGAA

TCGTTTAAAT

TTTAATAAAA

TAATGAGCAA

cGGcTrTTG EF117-4 (SEQ ID NO:43 6 EESLQNLK ETXQSXSE-TE LQNGDVRLNE YISLKGEIVE SDSRSSLIKK

GDRFILKSGS

SKYQVXNEQK KKLKIGDEVT VYGEYYGFLK GTLI ESEENH DSATN EF118-1 (SEQ ID NO:437)

TGAGGGGGAA

GCTTTCTTAC

AATCANAACG

TTTTCACAAG

AAAGACCCAT

CTTAGAGATrG

AGATAA

AAAGTGTGTT

TGACAGGTTG

CACAAGAATC

AAGATGGTGC

CGCTTTCTGG

AXATTAAAAG

AAAAAGAAAA

TGGCAAAAGT

TGGrCGTTI'GN

ACAAACGAAT

GGAAGATTCA

ccATGGGAAT

GTGGGGAITG

GCGAAAGATG

GATTTCTCTA

CCTATGAMrG

AGTAGATGCC

GGATGTACCG

TCGCAGGCGT TTCTGTTCA AGTTCATTCA

AGGAATCGGC

TGTCAATTAG

TGACATGAA-A

GGATGCTCAT

CACGCAAATC

AAAAAAGAAA AAGCATTCAA ATI'TCATTGG

TTGGATCGTT

EF118-2 (SEQ ID NO:438) VLKRXV GIVAGVFCSA LLLTGCGKSA KDEF IQGIGN XNAQESGVXD FSNSI SDM41F SQEDGAQTNP MIGMLITQIK DASLSGEDSS RCQKRKSIQL RDEIKSDGNG

CTDFIGWIVR

EF118-3 (SEQ ID NO:43 9 GAAAGATG AGTTCATTCA

AGGAATCGC

AATCANAACG CACAAGAATC TGGGGTTTGN4 GATTCTCTA TGTCAATTAG

TGACATGAAA

TTTTCACAAG AACATCCTGC ACAAACGAAT CCTATGATG CGATGCTCAT

CACGCAA-TC

AAAGACGCAT CGC=rCTGG GGAAGATTCA AGTAGATCC AAAAPLAGAAA

AAGCATTCAA

CT'TAGAGATG AAATIAAAkAG CGATGGGAAT GGATGTACCG ATTTCATTGG T GGATCGTT

AGAT

216 TABLE 1. Nucleotidc and Amino Acid Seqeuences of E. faecalis Genes.

EF118-4 (SEQ ID NO:440) KDEFIQGIGN XNAQESGVXD FSMSISD1M(F SQEDGAQTNP MIGMLITQIK DASLSGEDSS RCQKRKSIQL RDEIKSDGNG CTDFIGWIVR EF119-1 (SEQ ID NO:441)

TAAAGAATAC

AGTGCAACGG

GGCGGAGTGT

GTATCGACAG

TTTACGAGTG

GTAA.AACTTC

AATGAAAAGC

AAAACTGGAG

CATGAATATG

TTGGCTA-AGG

TATTAG

CGAGTAAAAT

AAAGGAGCTC

TGCTTGTTAG

AAGAAACGAC

AAGCAACTAA

TAGAAGCTTA

TAAACCCCCT

TAGCGTTAGT

cTTTACTTT

TGAAGAACAA

TTTCGGA;AGG

GGAAATCGTrG

TGGCTGTCAA

AGTGATI'GAA

AAAACAGACA

TGGAAATGCG

CATGACTGAA

TTCCGTAGGA

GGATGTGAA

TAAAATTTCT

CTMTTTCA

AATAAGCTAC

AGCCATAAGG

ACAGTAGCAA

GAAACAACGA

TATGCGAACT

AAATGTATCA

AAGGTTACAA

CAAAATGGAA

GAAATGACCT

AAAATTGTAT

CTTTACTTAT

AAGAAAACAA

GGGAACAATC:

AATTAGAAGA

TTACA.AGTAT

AAAAAAATGG

CGAT'-TATAA

CGCAGACACG

ATAATTCAGT

ATGCAAAAGA

ITTATTGTTA

GTCTAGTAAA

GAAGGAATCG

ACCAGATCAT

TAATGATC-GC

AATTGATGTT

AAATGATCA.A

AGTGTTACTT

TAAGCAAGAG

*q.

S S

S

S.

S.

S

.55S S. S S S

S.

EF119-2 (SEQ !D NQ:442) 'ZN KLPLLILLLG GVLLVSGCQS HKEENKSSKV STEETTVIET VAREQSKESF TSEATKXQTE TTKLEEPDHV KLLEAYGNAY ANFTSINDRN EKLKPLMTEK CIKK(NGIDVK TGVALVSVGK VTTI YKNDQH EYALLLDCEQ NGTQTRVLLL AXVKNNKISE MTYNSVKQEY EF119-3 (SEQ ID NO:443) AGAAAACAA GTCTAGTAAA GTATCGACAG AAGAAACGAC TTTACGAGTG AAGCAAkCTAA GTAAAACTTC TAGAAGCTTA AATGAAAAGC TAAAGCCCCT AAAACTGGAG TAGCGTTAGT CATGAATATG CTTTACTTTT TTGGCTAAGG TGAAGAACAA

TAT

AGTGATTGAA

AAAACAGACA

TGGAAATGCG

CATGACTGAA

TTCCGTAGGA

GGATI'GTGAA

TAAAATTTCT

ACAGTAGCAA

GAAACAACGA

TATGCGAACT

AAATGTATCA

AAGGTTACAA

CAAAATGGAA

GAAATGACCT

GGGPLACAATC

AATI'AGAAGA

TTACAAGTAT

AAAAAAATGG

CGATTTATAA

CGCAGACACG

ATAA LTCAGT

GAAGGAATCG

ACCAGATCAT

TAATGATCGC

AATTGATGT

AAATGATCAA

AGTGTTACTT

TAAGCAAGAG

EF119-4 (SEQ ID NO:444) ENKSSKv STEETrVIET VAREQSKESF TSE-ATKXQTE rITKLEEPDF4V KLLEAYGNAY ANFTS INDRN E-KLKPLMTEK CIKKNGIDVK TGVALVSVGK VTTIYKN~DQH EYALLJLDCEQ NGTQTRVLLL AXVKNKI SE MTYNSVKQEY EF120-1 (SEQ ID NO:44 5

TGAATAGGCG

ACTGCTTGTC

GTI'GAAGAGA

AGCAGTGTCG

AGTCAGCTAA

TTAGTTGGAA

TGAAAAAGGG

GGGAACCAAA

CGAATGAAAA

AGTCTAACGA

CTGTAGCTGA

TATGGAAAAA

AATGTTAGCG

AGNAAAGAAA

AACGAGTGAA

ATCAGTGAA-A

TTTAGATACT

TGGTAAAGGA

TT~TTGTCG

GTAACCGC T

ACAATTGATA

AATGAAGAC

ACAGCGATTA

CAGAGTTTGA

TrCCTAGCGGT

CAACGGAGGC

AGACAAACGA

CGACAGCTGA

ATGCTGGCGA

TCATTCATCC

TTTATCATTA

ATCCTCTAAA

ACAAGCGAGC

TGGAAAcAAT

TTITACTACT

TGATGGTAGT

TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

ACAAATACCG GAGGAATGAT TACGAAGGAT TCACCTACTG ATGAGTCGCG

ACCAATTACA

AGCTTAAGTA TTAGCTGGGG GCCTACTGGT GCTGCCCTAT TATTATATAA

AATTGGTGTT

EF120-2 (SEQ ID NO:446) VKKGMLAF FVVLAVLSLT ACREPKXKKV TASTEASSKV EETNEKTSET

IDKTNEQASS

SVE-SNESVKN EEPTADGNNS QLTVADLDTT AINAGDFTTL VGIWKNGKGE SLI IHPOGST NTGGMITKDS PTDESRPITS LSIRWGPTGA

ALLLYKIGV

EF120-3 (SEQ ID NO:447) AAGAAA GTAACCGCTT CAACGGAGGC

ATCCTC'AA

GTTGAAGAGA CGAATGAAAA AACCAGTGA-A

ACAATTGATA

AGCAGTGTCG AGTCTAACGA ATCAGTGAAA

AATGAAGAGC

ACTCAGCTAA CTGTAGCTGA TrAGATACT

ACACGATTA

TAGTTGGAA TATGGAAAAA TGGTAAAGGA

GAGAGTTTCA

ACAA.ATACCG GAGGAATGAT TACGAAGGAT

TCACCTACTG

AGCTTAAGTA TTAGGTCCGG GCCTACTGGT

GCTCGCTAT

AGACAAACGA

CGACAGCTGA

ATGCtGGA

TC:ATTCATCC

ATGAGTCGCG

TATTATATAA

ACAAGCGAGC

TGGAAACAAT

TTTTACTACT

TGATGGTAGT

ACCAATTACA

AATTGGTGTT

a.

a a a a a a EF120-4 (SEQ ID NO:448) KKV TASTEASSKV EETNEKTSET

IDKTNEQASS

SVESNESVKN EEPTADGNNS QLTVADLDTT AINAGDFTTL VGIWKNGKGE

SLIIHPDGST

NTGGMITKDS PTDESRPITS LSIRWGPTGA

ALLLIYKIGV

EF121-1 (SEQ ID NO:449)

TGAAACACAA

CATT=TTAA

GAAACAACGA

CAAGAACCAG

ACGAGTGACA

GAAACGTCAA

ACGTCAGATG

GTTGGTTTGT

ACCGTTTAA

AATAAACC

AAGTATGATG

AAAATTCAAC

GGTC ITCGTT

CCAGATITAA

CCTCGTGTTA

GAGTTGAAAG

AATAGTGATC

TATATTCTGG

ACTGTACCGG

GIrGCTAAAA ACGAATG ETC cGAGCGTTT'P

ATTAAAGGAP

GTTCAAAAAC

AAATTACCTC

ACcT1'AGTGI

GGAGGAAATT

TGGCTGTL'GC

GTCAACAAAG

TCATACACA

GTGTCAAGCA

TCGCTGAAALA

TTCATGGTCA

cccAAGTAAG

TTGATAATGG

CTTTAGTGAA

cAATGGTITT

AAGAAGCCAC

TrGTTGAAGG

AAGTTGGGAT

CTTCGCTTAA

CTAACGATCA

CGGCTGCCAG-

GTCATGACC.P

TAGGGGGACC

AT'GCCGATA-2

CAGCAGATGI

TTCAGGAGGI

LTTCCCGAAG(

AAGTAACGG(

CGGGGAAGX

k GTGTTCCCA'

TC;TGAAAAAG

GTTGATAGCG

'T~cAGAAGCA

GGAAACAACA

GTCACAAGAA

AGAAGAA.ACG

ATTATGGAAT

TACAGTCGTI'

CGACAATA T

TGAAAAGACC

GGGAAATCAT

TTTTCCAATC

GACTACCACG

TATCGGCTTA

TTTTTTACCT

GGCTGACAT'I

TGCCGACCAA

CCTI'TCTTTi

GAAAGATACC

GTGGGAAGTC

SAGCAGTTAAC

k GATCGGCAC) 'ACAATTACA2 3CGCACAAT~ r' 'TCCTATGC( r TAACGGTGA.

TTGAGCTTTA

CCAAGTGTTA

GTAACAAGTA

GACATCAAAC

ACCACAGCAC

AGCACGCCGC

TrGGTCTTATG

AACCAAGTCC

CAAGGCACTA

CATCCAATGA

GAGTTTAATT

TTGTCTGCGA

AAGGAACTTC

ACAATTCCG.C

TI'GAAAGA.AG

ATtGTTGC CI

*GTAATTGAA-P

*ACCAAGCAAC

GGGACAGAAC

CAAGAAGGTI

GCAGCGACAI

k GCAACAGCTC k CCAACAGCGj

AGTGCGGCA(

ACGA TTTD PL AACTTACTG, AAAAAGTGAA G

CTAGTACGGCA

CCACCGATTCA

AACAAGCACC

CAACAGAGAC

G

AAAAAATAAC

AAGATCATAA

GGGCACAAAAC

TTTTAACAGA

TCACCGCCATC

TTGGTTI'ACC

ATACCTACAA'

ATTITAATCA

ACATTCCTTT

AAGCAGAAAA

CCATrTCATGC

ATGTCGCGGG

GAGCAGCGCC

TTGTCAAAAT

SCAGCAACGAT

~AAGAATACCA

XITrTTTTACC k. TGATTTCTTT CGCTGmAA

ATATCTACAA

A, AGTATTTAGA

.TATGCAGTA

ACTAGAAAA

LAATCAGGCT

~ACGAATA

ATTITAGGT

LGAACTACCA

:CCAGCAGGC

EGACTTGTAT

;AATGTGATG

;TTAATCAM.A

I'AAGGAAGAT

ALGATGrGGCAG GTiGGGATGGC

AGCAGTTACT

GGGACAAcAA

GATTGATGCG

GAATGGAAAA

TGATCTTTCA

TGTACCAACA

TGAAAAAACG

AAAACAAGAA

AATTAATAAC

ATACGACAGT

ATACCCGAAT

AAAACAAGGG

218 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. foecalis Genes.

set**

GCGTACTATA

GTATATAACT

GGTGAACGAA

ACGA ETGCTA

GAACCTATTA

AAAAAAGGAA

AATTT1'GATG

ATTCCCACTT

CGTA.ATGCGG

GGCCGACTAG

CAAGAAAACC

AATTTCTCCA

GCGGTGGGAA

CTGAATTITC

GATCCGTATA

CCAGAAACAG

A IICCAGAAG

TTCGTGGTTA

ACGCTAGCAG

CATTCGCATA

CGTAATTATT

ACAACAAAAT

GCCATCGTTG

TATATTCCAT

GGGAATTTGA

GATTTTGCAG-

GTTGGGGATC

GGCGCCCAAC

AATGGCACA.J

CTACATGTT

GCTAGTGACC

GAAA ITAAAJ

ATGGCTACC)

CGAGAAGAA(

TTGCGGTJTG'

GGCGAACGJAj

ACGCCAAAA(

AATAAACAA.

TATGGCGGC'

CTGTTCGTD

TAG

ACCAAXACACA

ATGACATGAT

TTGTAGATGC

TGAATAATTA

AAAATTCTGA

CTCTTGATCC

AAAAATGGCG

CTC:CTGATGG

GCTTTGATITT

AAGCAGGGAA

CAACCTrGAT

AAGGCGCGGA

ATCACGAG71

CGATTTI'ATC

CAATCGTAGP

CA.ACGAAAAC

TAGAAGCAG~I

CTGGGCATT9)

AAACCCTTAC

CAGCCGTCGI

TAAATAATG'.

TGA'TTTCAGC

ATGAAGCAC(

*TCACATTGG

TITCGTGATG(

TAACTAATGC

TCATTGCTG'

TTAAAGAAA'

LTTTACTAG.

CAAGCTCCA

AAGGCAATC

kATCGGCAAA k ATGATTTCT 3 GATTTCAC r* ATCGTGCAG k. TTGTTTCTP

:CAGATCCAP

PL. ACCAAGCGC r TrTTAccT; 3 GACTTTCT9

GCCAGATGAT

TrTCTGGAGTG

GAA.AATTGAC

'TCGTTACGGC

TCCACAAACC

AGAACAAGAA

TGCCAAAGCA

ACGTACACCA

AGATAATGCA

AGGCGAATTA

GGTGGATGCA

TATGGCCAA-A

TGATTTTGGT

TAGTAATACG

AAAATCCGGG

ACACCCGAAA

GATTAAGGAA

AGGCGTAGAT

TCAAACATAI

AAGTGGCAAA

P TGGGATCGTC

-AGCAGAGCT'C

3 TACGAATTTI k. TGGACA.ACG;

-GATTATCTC;

3 TGGCGGCATr r GTTACCTTT' T GTTGAAATC A TGATGCTGG( T TCGTATCCA( A AACAGGACA C ACAAAAGTT' 'T AGCTGCTGG' T ACATTCTGTI C AACGACGAT ,T TTCGGAAGA hA ACCAGACCC ,G AGCAAGACA LA AACGGGTAC ,c TTCTGGCTG TGACCATTA G

GACTACAAGAT

GGCCAACCGC T GGTTTAGCTA G 1TACGAGGAA T

ATCGAACGAAA

ATCGAATI'AGT

AACGCCGCCG C

TATACCATTA

GGTATGGCGC G .CGGGATGTTT CCAATGAATC ITAGAGATTG C TATIACAAAG AAAAAGTTTG C

AACGTAGACA

ATTAAAGAGA

GAAACGACGC

CCTGAGTTAC

CGTTATGGCA

ACAGCACCAG

CTAGAATTGC

AACGCTGAAA

GAAAATGTGC

TATGGCCAAG

rCGCGCTCATA r' GGCAATAGCA 'TCTGTTCG LT

CAACCAAA.AC

TATGATTCCA

k. ACGATTGTCG r GAACCATrGG r GGTGATGG T

:T'TAATTGAAT

T GATTTAGCAC A GCITACAAAG G AAACCAACAC G AGCAATCCAT A GAAACAGAAA G TATATTTATA

TTTTAATCCA.

TGACATTTCk GGATCCT'-C C.

CCAAGGGAT T

GAT-TGTI'GAT

ETGGTCAAT

GAATGACGG C TATTACGAA A

CGCACACAAAT

TCTAAAAAC C 'CCAAGGATT A

LAGTTGCTA

ACTACGGTA T ~TGGC.AGTGG P

CATTGTAGG

GGTGACATT 9J kGTACGCGGA :GCATATCTG C

ATATCACTGT

AAGTGATCTA

A.GAGTGAACC

CAGAAAACCC

ATGAAAAAGT

GCACACGAGA

ACGCGTTTAG

TTAAACAACG

TTGCGCAAAT

CGATTCCACA

TTGGCGCAAA

CAAAACCAGG

GTAGTCGCGT

ATGAGAAGAA

ACGATATGCT

ACTTGAAAAG

AATATAAAGA

AGTTAATCGG

CAGAAACACC

CCGTAACAGA

CGCTTGCATT

AACGACGTAA

kACA I7CGT kAACCAGTG

~AACAATAT

CAAGTAGGG

TATATTAAG

A'I ICGGACA

ACTCTI'CAA

CAAGATGTC

rGACG ETCAT

TTA-AAGAC

,CCAATCTCC

,rGATGCCATG

~AAAGACCAA

LCGGGTT TTT

.GTGACGACC

~AAAGACCCG

'ATNCAAGCT

CGTGGTGAT

3ATTGATGGA rGCTCAAACA k.ACTAAGAAA

GGCACTTAAA

AATTGTCGAT

GACCAACTTA

CCAACCTGCT

GccAATTAAA

TCAAGTAACC

AAAAGATGAG

TGGTGGTTTC

TACTCGCTTG

ATTAGGAATA

ACAATACCG

AGGTGGTGAA

TGCAACCAGC

ACCATTCCCA

TGGAGGAGAG

AGTAGCAACC

GAAGAAAAAG

ATATGGTTTA

CAAAGCTAGT

0sS

S

0* S 9.

S.

EF121-2 (SEQ ID NO:450) VKKL SFKKVKWGMH FLMAVALIAP SVTSTAYAVE TTSQQSSE-AV TSTTDS

EPVITQETTD

SDVHGQLWNW

KAPLVNEKTH

LRFVEGTTTK

LKANDQADI I

VPVGGPKDTG

AFIQEEIGTA

LPAGKI SYAT

IKQEAPNQAT

SYEDDKELPV

PMITAMNVMK

ELDFNQDGQP

VAS IHAGQQN

TEVVKIDLSV

TADFLPKQEI

IFDIYKYPNT

SDSVKQSQET

GLSQVSTVVN

YDAMVLGNHE

DLKVGIIGLT

SDPAASADQV

AKNADKWEVQ

I(GIPEAQLQP

LVSVPIbIGEN ER IVDAXI DG TAPTETTNriE

QVRAQNPAGT

FNFGLPLIKK

IPHIPLWDGP

IENVAGIDAY

EGTATIVPTT

TAMISLINNV

LLKYLEKQGA

QPLDPAKEYT

VLIDNGDNIQ

IQQEATFP IL

RVTSLNFLPL

ILGHDHLSFT

NVPADEAVKA

QKEVTGAQLS

YYNQTQPDDL

IAMNNYRYGG

SRKQ

TPQKITILGT

GTILTDDLYN

SANTYNKEDG

KEEAEKAVTE

KQGAAPN1GY(T

ATKEYHF.KTR

AAALFKYDSK

TISFNPNIRV

LASQGIQVGE

YNYDMISGVD YKIDISKPVG 219 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

PIKNSDPETL

PTSPDGRTPN

ENPTLMVDAG

NFP ILSSNTY

PEVEAVIKEI

SHTAVESGKR

IVDE-ARTNFN

FAVTNGGG IR

GTILLDDAGQ

IKNRQTQKFE

RLYRAATTID

KQNQAGARQS

RGMIVDYIKK

AAAITKQDVR

DVFQGLPISN

YKDGSGRVFD

KEKYADXQAF

YGKVIYAQTG

AENEKVIVDY

ADIKQGPIKV

PKLGANGGFL

PLDEKKQYRM

LAQYKEPFPG

NP SVTZKKKY

KGTLOPEQEI

NAG FDLDNAY

FSKGADMAKA

PYTIVEKSGK

VVTGHLGVDE

NYLNNVG IVT IPFTLDGQR-7

GOVIAVLPFG

HVSSSIRIHY

ATNDFLAAGG

ERIVSISEE-A

GGFLPKTGTE

ERNWSI IGTN

TIMHTNDV{G

MNEVG.YDAMIA

KFAIVGVTI'P

'1 9 1PH IWRGDT

APESEPTKKT

NVRTRETNLG

NSIAQIQVTG

DSTKPGTR{LA

DGYDMLGGER

YKYELIGGGET

TETLALYGLL

FDEKWRAKAI

RLEAGKGE:LG

VGNHEFDFGL

E-TATKTHP"!

LAETLSQTYP

TKL ISAAELL

NLIGDAIMSY

AQVKEMFEMS

SDEGNETGQT

EEGISLDSVL

PKPDPKPDPK

FVGLS SSGWY

ELVNDGTLQI

MARLKTFKDQ

EIALGYXDQL

VEKVTFKDP I

ELDITVIDGH

ELPENPAVKA

GQDAFSQPAO

vRS IPQKDEN

IVGSRVLGIE

IEYLKSATSL

PTPETPVATN

IYKRRNKAS

EF121-3 (SEQ ID NO:451) ACAAAG TTCAGA.AGCA GTAACAAGTA CCACCGATTC AAGI *9 0 90

SS

S S

SS

SS

S 0 5@ 5* 0 5

S

S.

0 5 5

CAAGAACCAG

ACGAGTGACA

GAAACGTCAA

ACGTCAGATG

GTTGGTTTGT

ACCGTTTTAA

AATAAAG.CGC

AAGTATGATO

AAAATTCAAC

GGTCTTCGI'I

CCAGATTTAA

CCTCGTGTTA

GAGTI'GAAAG

AATAGTGATC

TATA'ITCTGG

ACTGTACCGG

GTTGCTAAAA

ACGAATGTTC

CGAGCG=IA

ATTAAAGGAA

GTTCAAAAAG

AAATTACCr

ACCTTAGTGA

GCGTACTATA

GTATATAACT

GGTGAACGAA

ACCAT'rGCTA dAACCTATTA

AAAAAAGGAA

AATTTTGATCG

ATTCCGACTI

TCATTACACA

GTGTCAAGCA

TCGCTGAAAA

'ITCATGGTCA

CCCAAGTAAG

TITGATAATGG

CT'1AGTGAA

CAATGGTTTT

AAGAAGCCAC

'FrGTTGAAGG

AAGTTGGGAT

CTTCGCTTAA

CTAACGATCA

CGGCTGCCAG

GTCATGACCA

TAGGGGGACC

ATGCCGATAA

CAGCAGATGA

TTCAGGAGGA

'rrCCCGAAGC

AAGTAACGGG

CGGGGAAGAI

GTGTTCCCAI

ACCAAACAC;

ATGACATGA1

TTGTAGATGC

TGAATAATTI

AAAATTCTGI

CTCTTGATC(

AAAAATGGCC

CTCCTGATG(

GGAAACAACA

GTCACAAGAA

AGAAGAPLACG

ATTATGGAAT

TACAGTCGTT

CGACAATATT

TGAAAAGACC

GGGAAATCAT

TITTCCAATC

GACTACCACG

TATCGGCTTA

TTTTTTACCT

GGCTGACATT

TGCCGACCAA

CCTTTCTT7I

GAALAGATACG

GTGGGAAGTG-

AGCAGTTAAC

GATCGGCACP

ACAATTACAP

iCCCACAATT;

TTCCTATGCC

TAACGGTGA.U

GCCAGATGK.

TTCTG.GAGTC

GAAAATTGA(

TCGTTCGG(

k. TCCAGAAAC( AGAACAAGXi 3TGCCAAAGC 3ACGTACACC,

GACATCAAAC

ACCACAGCAC

AGCACCCC

TGGTCTTATG

AACCAAGTCC

CAAGGCACTA

CATCCAATGA

GAGTTTAATT

TTGTCTGCGA

AAGGAACTI'G

ACAATTCCGC

TTGAAAGAAG

ATIGTTGCCT

GTAATTGAAA

ACCAAGCAAG

GGGACAGAAG

CAAGAAGGTA

GCAGCGACAA

GCAACAGCTC

CCAACAGCGA

AGTGCGGCAG

ACGATTFIG

kAAC'ITACTGA.

C TTGACCATTP 3GACTACAAGP

GGCCAACCGC

2 GGTTAGCV.

TI'ACGAGGA;

N ATCGAACGA) N. ATCGAATTAC A, AACGCCG

'GAAAA

AAGAAGCACC

CAACAGAGAC

AAAAAATAAC

AAGATGATAA

GGGCACAAAA

T=T1AACAGA

TCACCGCCAT

TMG=ACC

ATACCTACAA

AITTTAATCA

ACATI'CCTTI'

AAGCAGAAAA

CGATI'CATGC

ATGTCGCGGG

GAGCAGCGCC

TTGTCAAAAT

CAGCA.ACGAT

AAGAATACCA

AITITTTTACC

TGATTCTTT

*CGCTGTTTAA

ATATCTACAA

AGTAI'TTAGA

GTTTTAATCC

ITACATLTC

TGGATCCTGC

GCCAAGG-GA]

TGATTGTTGrz k ATTGGTCA-A'

TGAATCGACG(

AAATCAGGCT

GACGAATITA

AArI'TTAGGT

AGA.ACTACCA

CCCAGCAGGC

TCACTTGTAT

GAATGTGATG

GTTAATCAAA

TAAGGAAGAT

AGATGGGCAG

GTGGGATGGC

AGCAGTTACT

GGGACAACAA

GATTGATGCG

GAATGGAAAA

TGATCTTTCA

TGTACCAACA

TGAAAAA.ACG

AAAACAAGAA

AATTAATAAC

ATACGACAGT

ATACCCGAAT

AAAACAAGCG

AAACAITCGT

AAAACCAGTG

CAAAGAATAT

7TCAAGTAGG

TTATATTAAG

r TATTGGGACA 3CACTC'FrCAA

S.

OS@S

S

9

OSSO

OSSO

S

0000 @0 5 00 0S EF121-4 (SEQ ID NO:4S2) QSSEAV TSTTOSSRKQ EPVITQETTD IKQE-APNQAT SDSVKQSQET TAPTETTNLE TSIAEKEETS TPQKITILGT SDVHGQLWNW SYEDDKELPV GLSQVSTVVN QVRAQNPAGT VLIDNGDNIQ GTILTDDLYN 220 TABLE 1. Nucleotide and Amino Acid Seqeuences; of E.faecalis Genes.

KAPLVNEKTH

LRFVECTTTK

LKANEDQADI

I

VPVGGPKDTG

AFIQEEIGTA

LPAGKISYAT

YNYDMI SGVD

PIKNSDPETL

PTS POGRTPN

PMITAMV'

ELDFNQDGQP

VAS IHAGQQN

TEVVKIDLSV

TADFLPKQEI

I FD IYKYPNT

YKIDISI(PVG

RGMIVDYIKK

YDAMVLGNHE

OLKVGIIGLT

SDPAASADQV

AKNADKWEVQ

KGIPEAQLQP

LVSVP INGEN

ERIVDAKIDG

KGTLDPEQEI

FNFGLPLIKE

IPHIPLWDGP

I ENVAGIDAY

EGTATIVPTT

TAMI SLINNV

LLKYLEKQGA

QPLDPAKEYT

ERNWS IIGTN

IQQEATFIL

RVTISLNFL-PL

ILGHDHLSFT

NVPADE-AVKA

QKEVTGAQLS

YYNQTQPDDL

IAMNNYRYGG

FDEKWRAKAI

SANTYNKEDG

KEEAEKAVTE

KQGAAPNGKT

ATKEYHEKTR

AAALFKYDS(

TISFNPNIRV

LASQGIQVGE

ELVNDGTLQ

I

EF122-1 (SEQ ID NO:453)

TGAAACACAA

CATTTTTTAA

GAAACAACGA

CAAGAACCAG

ACGAGTGACA

GAAACGTCAA

ACGTCAGATG

CTTGGTTTCT

ACCGTTFTAA

AATAAAGCGC

AAGTATGATG

AAAATTCAAC

GGTCTTCGTT

CCAGATTTAA

CCTCGTGTTA

GAGTT1GAAAG

AATAGTGATC

TATATTCTG

ACTGTACCGG

GTTGCTAAAA

ACGAATGTTC

CGAGCG'ITTA

ATTAAAGGAP.

GTTCAAAAAC

AAA TACCTC

ACC~TTAGTG;

GCGTACTATI

GTATATAAC9

GGTGAACGAI

ACGATTGCTJ

GAACCTATTj

AAAAAAGGAJ

AATTTTGATI

AI'CCGACTI

CGTAATGCG,

GGCCGACTA

CAAGAAAAC

AATTI'CTCC

GCGGTGGGA

CTGAATTTI

GGAGGAAATT

TGGCTGTTGC

GTCAACAAAG

TCATTACACA

GTrTCAAGCA

TCGCTGAAAA

TTCATGGTCA

CCCAAGTAAG

TTGATAATGG

CTI'TAGTGAA

CAATGGTTTT

AAGAAGCCAC

TTG7'rGAAGrG

AAGTTGGGAT

CTTICGCTTAA

CTAACCATCA

CGGCTGCCAG

GTCATGACCA

TAGGGGGACC

ATGCCGATAP

CAGCAGATGP

ITCAGGAGG;

'FrCCCGAAGC

AAGTAACGGC

CGGGGAAGA'.

GTGTTCCCK

SACCAAACACi

LATGACATGA!

k. TTGTAGATGi

TGAATAATI'

AAA-ATTCT.

k CTCTTGATC S AAAA.ATGGC T CTCCTGATG G GCTTTGA7I G AAGCAGGGA C CAACCTrGA A AAGGCGCGC A ATCACGAGI 'C CGATT'ITA)

TGTGAAAAAG

GTTGATAGCG

TTCAGA.AGCA

GGAAACAACA

GTCACAAGAA

AGAAGAA.ACG

A'ITATGGAAT

TACAGTCGT'

CGACAATATT

TGAAAAGACC

GGGAA.ATCAT

TTTI'CCAATC

GACTACCACG

TATCGGCTTA

TTTTTACCI

GGCTGACATI

TGCCGACCAP

CCTTTCTI'

GAAAGATACC

GTGGGAAGT(C

AGCAGTTAAC

LGATCCGCACJ

ACAATTACAj

CGCACAATT

C' TTCCTATGCI r' TAACGGTGIk k GCCAGATGA r TTCTGGAGT

-GAAAATTGA

h TCGITACGG A TCCAGAAAC C AGAACAAGP G TGCCAAAGC G ACGTACACC T AGATAATGC A AGGCGAATI 6T GGTGGATGC ;A TATGGCCPJ .r TGATTTTG .C TAGTAATA( ;A AAAATCCGI

TGAGCTTTA

CCAAGTGTTA

GTAACAAGTA

GACATCAAAC

ACCACAGCAC

AGCACGCCC

TGGTCTTATIG

AACCAAGTCC

CAAGGCACTA

CATCCAATGA

GAGT"TTAATT

TTGTCTGCGA

AAGGAACTTG-

ACAATTCCGC

TTGAA-AGAAC

ATTGTTGCC']

LGTAATGAAN

ACCAAGCAAC

GGGACAGAA(

CAAGAAGGT

GCAGCGACAX

k GCAACAGCT k. CCAACAGCG.

k~ AGTGCGGCA

ACGATTTTT

A AACTIACTG T FI'GACCATT G GACTACAAG C GGCCAACCG C GGTlTAGC'I C TTACGAGGP LA ATCGAACG; :A ATCGAATI :A AACGCCGCC :A TATACCAT.

LA GGTATG-GCC :A GGGGATG7 k.A GCAATGAA! 'T TTAGAGAT :G TATTACAA AAA.AAGTGAA G' CTAGTACGGC

A)

CCACCGATTC

AJ

AAGAAGCACC CAACAGAGAC

G~

AAAAA.ATAAC

X~

AAGATGATAA

A(

GGGCACAAAA

C

TITTAACAGA T1 TCACCGCCAT

G.

TTGGTI'TACC

G

ATACCTACAA

T

ATTTTAATCAA

ACATTCCTTT

G

AAGCAGAAAA

7CCATTCATGC

G

kATGTCGCGGG

G

'GAGCAGCGCC

G

TTGTcAAAAT k CAGCAACGAT k. AAGAATACCA 2AT'FITIACC A. TGATTTCTTT 0 CGCTGTITAA G ATATCTACAA A AGTATTI'AGA A GT=11AATCC A TTACATTC ;C TGGATCCTGC

GCCAAGGGAT

LA TGATTGTTGA A ATTGGTCAAT kG TG.AATGACGG 'G CTAITACGAA C~A TCCACACAAA ;C GTCTAAAAAC rT TCCAAGGATT ro AAGTI'GGTTA TG CACTAGGTI'A AG ATGGCAGTGG

.GGGGCATG

'ATGCAGTA

~TAGAAAA

kATCAGGCT kCGAATTTA

~TT

T

ITAGGT

GAACTACCA

CCAGCAGGC

2ACTTGTAT

AATGTGATG

TTAATCAAA

AACGAAGAT

GATGGGCAG

TGGGATGGC

,GCAGTTACT

GGACAACAA

ATTGATGCG

;AATGGAAAA

7GATCTTTCA

E'GTACCAACA

rGAAAAAACG kAAACAAGAA k.ATTAATAAC kTACGACAGT

ATACCCGAAT

P.AAACAAGGG

AAACATTCGT

AAAACCAGTG

CAALAGAATAT

TCAAGTAGGG

TrATATTM-G

TATTGGGACA

CACTCTTCAA

ACAAGATGTC

TGACGTTCAT

CTTTAAAGAC

ACCAATCTCC

TGATGCCATG

TAAAGACCAA

ACGGGTTTITT

AAAAAGTTTG CCA'FI'TAGG TGTGACGACC GATCCGTATA

CAATCGTAC

221 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.f[aecalis Genes.

CCAGAAACAG

ATTCCAGAAG

TTCGTGGTTA

ACGCTAGCAG

CATTCGCATA

GGTAATTATT

ACAACAAAAT

GCCATCGTI'G

TATATTCCAT

GGGAATTGA

GATTTTGCAG

G PIGGGGATG

GGCGCCCAAG

AATGGCACAA

CTACATGTIT

GCTAGTGACG

GAAATTAAAA

ATGGCTACCA

CGAGAAGAAG

TTGCGGTTGT

GGCGAACGAA

ACGCCAAAAC

AATAAACAAA

TATGGCGGCI

CTGTTCGT'G

CAACGAAAAC

TAGAAGCAGT

CTGGGCA'TIT

AAACCC LTAG.

CAGCCGTCGA

TAAATAATGT

TGATTTCAGC

ATCAAGCACG

TCACATTGGA

TTGGTGATGC

"TAACTAATGG

TCATTGCTrGT

TTAAAGAAAT

TTTTIACTAGA

CAAGCTCCAT

AAGGCAATGA

ATCGCCAAAC

A EGATTITCTT GGAqTTCACT

ATCGTGCAGC

TTGTTTCTA'I

CAGATCCAAP

ACCAAGCGGG.

T=TACCTAM

GACTTTCT'C

ACACCCGAA-A

GATTAAGGAA

AGGCGTAGAT

TCAAACATAT

AAGTGGCAAA

TGGGATCGTC

AGCAGAGCTG

TACGAATTTT

TGGACAACGA

CATTATGTCA

TGGCGGCATT

GTTACCTTTT

GTTTGAAATG

TGATGCTGGC

TCGTATCCAC

AACAGGACAA

ACAAAAGTTI'

AGCTGCTGGT

AGATTCTGTC

*AACGACGATT

T~CGGAAGAA

ACCAGACCCC

AGCAAGACAC.

AACGGGTACP

'TCTGGCTGC

AACGTAGAGA

ATTAAAGAGA

GAAACGACGC

CCTGAGTTAG

CGTTATGCCA

ACAGCACCAG

CTAGA.ATTGC

AACGCTCAAA

GAAAATGT4GC

TATGGCCAAG

CGCGCTGATA

GcAATAGCA

TCTGTTCGTT

CAACCAAAAC

TATGATTCCA

ACGAI 'GTCCG GAAC CATTGC GGTGATGGT I T-rAATTAAWJ

*GATTTAGCAC

*GCTTACAA.AC

AAACCAACAC

AGCAATCCA'

GAAACAGAA2 TATATTTATj

AGGTGACA-T

AGTACGCCGGA

CGCATATCT-G

ATATCACTGT

AAGTGATCTA

AGAGTGAACC

CAGAAAACCC

ATGAAAAAGT

GCACACGAGA

ACGCGTTTAG

1'TAAACAAGG

T'TGCGCAAAT

CGATTCCACA

'FrGGCbCAAA

CAA.AACCAGG

GTAGTCGCGT

ATGAGAAGAA

ACCATATGCT

ACTTGAAAAG

AATATAA.AGA

AGTTAATCGG

CAGAA.ACACC

r CCGTAACAGA k. CGCTTGCATI AACGACGTAAi

TAAAGACCCG

TATNCAAGCT

GCGTGGTGAT

GATTGATGGA

TGCTCAAACA

AACTAAGAAA

GGCAGTTAAA

AATTGTCGAT

GACCAACTTA

CCAACCTGCT

GCCAATTAAA

TCAACTAACC

AAAACATGAG

TGGTGGTTTC

TACTCGCTTG

ATTAGGAATA

AcAATACCGG

AGGTGGTGAA

TGCAACCAC

ACCATTCCCA

TGGAGGAGAG

AGTAGCAACC

GAAGAAAAAG

ATATGGTTTA

CAAAGCTAGT

a

C

4~*

TAG

ZF122-2 (SEQ ID NO:454) VKKL SFKKVKWGMH FLMAVAL lAP SVTSTAYAVE TTSQQSSEAV

TSTTDSSRKQ

EPV ITQETTD

SDVHGQLWNW

KAPLVNEKTH

LRFVEGTTTK

LKANDQADI I

VPVGGPKDTG

AFIQEEIGTA

LPAGKISYAT

YNYD)MISGVD

P IKNSDPETL PTS PDGRTPN

ENPTLMVDAG

NFPILSSN'TY

PEVEAVIKEI

SHTAVESGKR

IVDEARTNFN

FAVTNGGG IR

GTILLDOAGC

IKNRQTQKFE!

RLYRAATTIE

KQNQAGARQS

IKQEAPNQAT

SYEDDKELPV

PMITAM4NVMK

ELDFNQDGQP

VAS IHAGQQN

TEVVKIDLSV

TADFLPKQEI

IFDIYKYPNT

YKIDISKPVG

RGMIVDYIKK

AAAITKQDVR

DVFQGLPISN

YKDGSGRVFD

KEKYADXQAF

YGKVIYAQTG

AENE-KVIVIDY

ADIKQGPIKV

PKLGANGGFIL

PLDEKYQYPYJ

LAQYKEPFPC

NPsvTEKKIXN

SDSVKQSQET

GLSQVSTVVN

YDAMVLGNHE

DLKVGI IGLT

SDPAASADQV

AKNADKWEVQ

KGIPEAQLQP

LVSVPINGEN

ERIVDAKIDG

KGTLDPEQE I

NAGFDLDNAY

FSKGADMAKA

PYTIVEKSGK

VV'TGHLGVDE

NYLNNVCIVT

IPFTLDGQRE

GDVIAVLPFG

HVSSSIRIH'Y

IATNDFLAAGG

ERIVSISEEA

GGFLPKTGTE

QVRAQNPAGT

FNFGLPLIKK

IPHIPLWDGP

IENVAGIDAY

EGTATIVPTT

TAMI SLINNV

LLKYLEKQGA

QPLDPAKEYT

ERNWSI IGTN

TIM}{TNDVHG

MNEVGYI)AMA

KFAIVGVTTP

TTPH IWRGDT

APESEPTKKT

NVRTRETNLG

NS IAQIQVTG

DSTYPGTRLA

DGYDNLGGEP

YKELIGGGEl

TETLALYGLI

VLIDNGDNIQ

IQQE-ATFPIL

RVTSLNFLPL

ILGHDHLSFT

NVPADE.AVKA

QKEVTGAQLS

YYNQTQPDDL

IAMNNYRYGG

FDEKwRAKAI

RLEAGKGELG

VGNHEFDFGL

ETATKTHPKN

L.AETLSQTYP

TKLI SAAELL

NLIGDAIMSY

AQvKEMFEMKS

SDEGNETGQT

EEGISLDSVL

PKPDPKPDPK

FVGLSSSGWY

TPQKITILGT

GTILTDDLYN

sANTYNKEDG

KEEAEXAVTE

KQGAAPNGKT

ATKEYHEKTR

AAALFKYUSK

TISFNPNIRV

LASQGIQVGE

EL VNDGTLQ I

MARLKTFKDQ

EIALGYKDQL-

VEKVTFKDP I

ELDITVIDGH

ELPENPAVXKA

GQDAFSQPAD

vRs IPQKDEN IVGSRVLG

IE

IEYLKSATSL

PTPETPVATN

IYYRRNKAS

EF122-3 (SEQ ID NO:45 5 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

TG AAAAATGGCG TGCCAAAGCA ATCGAATTAG TGAATGACGG

CACTCTTCAA

ATTCCGACTT

CGTAATGCGG

GGCCGACTAG

CAAGAAAACC

AATTTCTCCA

GCGGTGGGAA

CTGAATTTTC

GATCCGTATA

CCAGAAACAG

ATTCCAGAAG

TTCGTGGTTA

ACGCTAGCAG

CATTCGCATA

GGTAATTAT

ACAACAAAAT

GCCATCGTTG

TATATTCCAT

GGGAkATTTGA

GATTTTGCAG

GTTGGGGATC

GGCGCCCAAG

AATGGCACAA

CTACATGTTT

GCTAGTGACG

GAAATTAAAA

ATCGCTACCA

CGAGAAGAAC

TTGCGGTTGE

GGCGAACGA6P

ACGCCAAAAC

AATAAACAAP

CTCCTGATGG A GCTTTGATTTI A AAGCAGGGAA

A

CAACCTTGAT G

AAGGCCCGGA

ATCACGAGTT I1 CGA=TTTATC

CAATCGTACA

CAACGAAAAC

TAGA6AGCAGT CTGGGCA1TT

AAACCCTTAG

CAGCCGTCGA

TAAATAATGT

TGATTTCAGC

ATGAAGCACG

TCACATTGGA

ITGGTGATGC

TAACTAATGG

TCATTGCTGT

TTAAAGAAAT

TITTACTAGA

CAAGCTCCAT

AAGGCAATGA

ATCGGCAAAC

ATGATT"TCTT

GGArI'TCACT

ATCGTGCAC

TTGTTTCTAT

CAGATCCAAA

LACCAAGCGGG

.CGTACACCA

GATAATGCA ~GGCGAATTA G

;GTGGATGCAC

.ATGGCCAAA C ~GATTTTGGT

'GTAATACG

".ATCCGGG

kCACCCGAA6A 3ATTA6AGGAA kGGCGTAGAT rCAAACATAT k~AGTGGCAAA

TGGGATCGTC

AGCAGAGCTG

TACGAATTTT

TGGACAACGA

GATTATCTCA

TGGCGGCATT

GTTACCTTTT

GTTTGAAATG

TGATGCTGGC

TCGTATCCAC

A6ACAGGACAA

ACAAAAGTTT

AGCTGCTGGT

AGATTCTGTC

AACGACGATT

TTCGGAAGAA

ACCAGACCCG

AGCAAGACAG

LACGCCGCCG

.ATACCATTA

;GTATGGCGC

;GGGATGTTT

;CAATGA6ATG

.TAGAGATTG

C'ATTACAAAG

kAAAAGTTTG

),,ACGTAGAGA

%TTAAAGAGA

GAAACGACGC

CCTGAGTTAG

:GTTATGGCA

ACAGCACCAG

CTAGAATTGC

AACGCTGAAA

GAAAATGTGC

TATGGCCAAG

CGCGCTGATA

GGCAATAGCA

TCTGTTCGTI

CAACCAAAAC

TATGATTCCP

ACGATTGTCC

GA.ACCATTGC

GGTGATGG J

TTAATTGA.A

GATTTAGCA(

GCTTACAA6A(

AAACCAACA(

AGCAATCCA'

CTATTACGALA ACAAGATGTC TGCACACAAA TGACGTTICAT GTCTAAAAAC CTTTAAAGAC .TCCAAGGAT ACCAATCTCC AAGTTGGTTA TGATGCCATG CACTAGGTTA TAAAGACCAA ATGGCAGTGG ACCGGTTFI' CCATTGTAGG TGTGACGACC AGGTGACATT TAAAGACCCG AGTACGCGGA TATNCAAGCT CGCATATCTG GCGTGGTGAT ATATC ACTGT GATTGATGGA AAGTGATCTA TGCTCAAACA AGAGTGAACC AACTAAGAAA CAGAAAACCC GGCAGTTAA-A ATGAAAAACT

AATTGTCGAT

GCACACGAGA GACCAACTI'A ACGCGTTTAG CCAACCTGCT TTAAACAAGG GCCAATTAA6A TTGCGCAAAT TCALAGTAACC CGATTCCACA AAAAGATGAG rI'GGCGCAA.A TGGTGGTTI'C CAAAACCAGG TACTCGCTTG GTAGTCGCGT ATTAGGAATA ATGAGAAGA6A ACAATACCGG ACGATATCCT AGGTGGTGAA P' ACTTGAAAAG TGCAACCAGC AATATAAAGA ACCATTCCCA AGTTAATCGG TGGAGGAGAG CCAGAAACACC AGTAGCAACC T CCGTAACAGA CAAGAAAAAG TATGGCGGCT TT EF122-4 (SEQ ID NO:456) 4* EKWRAXAI ELVNDGTLQI PTSPDGRTPN AAAITCQDVR ENPTLMVDAG DVFQGLPISN NFPIhSSNTY YKDGSGRVFD PEVEAVIKEI KEKYADXQAF SHTAVESGKR YGKVIYAQTG IVDE-ARTNFN AENEKVIVDY FAVTNCGIR ADIXQGPIKV GTILLDDAGQ PKLCANGGFL IKNRQTQKFE PLDEKKQYRM RLYRAATTID LAQYKEPFPG KQNQAGARQS NPSVTEKKKY

NAGFDLDNAY

FSKGADMAKA

PYTIVEKSGI{

VVTGHLGVDE

NYLNNVG IVT IPFTrJDGQRE

GDVIAVLPFG

HVSSSIRIHY

ATNDFLAAGG

ERIVSISEEA

GGF

TIMHTNDVHG

MNEVGYDAMA

KFAIVGVTTP

TTPH IWRGDT

APESEPTKKT

NVRTRETNLG

NS IAQIQVTG

DSTKPGTRLA

[)GYDMLGGER

YKEL IGGGET

RLEAGKGELG

VGNHEFDFGL

ETATKTHPKN

LAETLSQTYP

TKLISAAELL

NLIGDAIMSY

AQVKEMFEMS

SDEGNETGQT

EEGISLDSVL

PKPDPKPDPK

MARLJKTFKDQ

EIALGYKDQL

VEKVTFKDPI

ELJDITVIDGH

ELPFNPAVKA

GQDAFSQPAD

VRSIPQKDEN

IVGSRVLGIE

IEYLKSATSL

PTPETPVATN

EF123-1 (SEQ ID NO:457) TAAAATAAAA AATTGGTACG AAGTGAACGT TCTC'I7CTAT GTGTCGTTAG TAGAGGAAGG ATGAAAGAAA TGAGAAAGAA TGGTCCAATG GTAAACCGTT GGCTCTACGG GrI'GATGTGT 223 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

TTGTTACTTG

GATGGCCACT

CTI'CAAGGAA

TCAATCAAAG

CAGAAGAAAC

ACGTTGAACC

TTGACGTTAA

GCATCAGCGA

TCCGAAGAAA

ACTGTCAAAC

ACA.ACGATTA

ACACCGCCGT

GATGTGCGAG

AAACCTAATA

TACACAATTA

AGTGACATTC

CCAGGAGATT

ATTGTCCCAG

CCTAGTGCGA

ACTGTGACGG

GTGATGAATC

ACCGTTGATA

GAGTACCAAA

AAAA.ATCACC

GTTACCGCCP

CAAGAAT'TC

GCAGTCATTI

TATTCAGTGI

AAAGATTACI

GTGACTGGCC(

GTTGCCGTGJ

GCCAGTCAA(

GGTTGGACG!

ACGTACGAA(

ACCACTGGI)

GATGGTGAA

TCCACATA

TTGGACCAT

CATCAGAA

AGCGGTGTT

AATAATCGT

GCTGGGAGC

AAACCAACG

ACTTGGCG'I

GTTGATGAP

CAAGGTTC'I

AAAAAAGG I

AATGGCGCC

GTGCTAGAJ

ACGCCAGAJ

GATAATGAJ

TATATGGAJ

AACCAAGT(

GTGGTCGT'

TTCTAAATTA TG TAACGTTAGG AG AAGCACAACC AG CTGCACATTG GG AGATTCAAAT TG CTACAGCTAC

AG

AGACTGGTAC

TG

ATGAAGGTTC A-P CTGTCGCCAG CP CGCGCGTAGC

AC

TCGATAATTT TC ATAAAGAAGA

TC

A.ACAAATGAA AC AACCAIGTTC AC GTGAAGATGG T~ ACGGGGAC ET 7 GGGTGATTGA Tj ATACCGAACA

A

FITAC ETGGAC G~ AAACATGGCC A.

TTGATGGAAC

A

AAAATGGCAA

T

CGACGATGA

C

CGAcGTTAACA

CATATGGCAAA

CTTGGGAALATT

CAGACACAAT

kCATITGATGA

C

.AAGTGGTAAT C ;CAGTCAAGAT T1 ~ATAATCGTGT

C

AA.AATATTATJ

r TAGCTGTGAA 9J -CAGTTCCTGG

C

3 CTCAGTTGACC A CAGGCTI'TAAC A CFI'ATACTAC T ATCGAAATAC G ATAGTAAACC T ACAATGCCGT T TAGTCGACGC T TGAAAGTCTA ;C AACCGTTGAC rG 'TGATTTCC LA AAG~rATCGA

CACGTGATGT

GCGAATACCA

'C AATCGGTITI'- M' CCGAGTCATI EA AATCTGTAT kA CAGGACAACA k.T ATCGTACTTT IT cAATTACTGG rG ACATTGATCA GCACACCA CTCATGGCTT AAGTGAAG CAAACCAGCCC TAACACAA GAGGTTGTAG CAGCGCCC AATAATACGC AATTGAAT cAGCAAGCGT AAGATGTG ACGTFITCT ~ATCCGACA GAATCA.ACGG ~CACAAGAA GCATCTACAA ~CGACAAAA GCGATAGAAA ;GACCAACA GATATCAGTG ;AAGATCCG _ATTTAITTAA TGACCATT CATTGAACT CAGGCGAT TAC~rCGAGT -GTGATTTA GTTGATIGCAG DLCGGTTCGT 1'TTACCTI'TA FCTTTAGAT ACTCAT=GA ~TTCCTACA CAAGAAGATT :AA.ATTGAT AAACAAGGCC GTAGATATC A.ATCAAGCGA ACAGGGAAT ACCTTTA-AGI TAAAGAA GTGC-GTCGCC GTGACGAT

AAAGGTGACP

GAGGCGGTT ATTCCAGATC AGTGATAAT AATCC-AAATC ATGTTAGAC AAGCGCAATI 'AACTACAAC TATGGTGAAC GGGGATAAT TTAACG=I :AAAGGAAAT

GAAGTCGTTC

AACGGAGAC GGTTCCIn ~GATI'ATAALA ACCAAAGTT GATGTTGGC ACTGGTCAGI *AAAAACACT GGTGCAGTfl rCAAAATAAT TA TTTGATG TTAACTATG GTACCCAAT ;TTAGGCAAG

GATTTCATG

'GTAAG=~T

ATAGGGGCG

CTTTTTCGAT GTTACCGAG; CGCTGCCATT GATTGGACG GITAAACCT

TTACCTGCI

CACCAALAGA.A ATCACTTGC CTTTrrrGACG GATCCAAT] TGAAGGCAAT

ACAAAGCC.

GGATATCACA ATGGAAGMJ TA-ATGATAGT

CGTACGTA'

AGGTTCGGCT AG ETATGA( GACAGGAAAA GT'rrCTA7 CAAAGATGAT CCAGATCA' AGACGATGTG GTTATTAC' GGTGATTTAC GGTACCAA TTTAGAAGAA GGAAAAGX AAAA.ATTGTC- GTTAAA-AT AGTGACVTCT TCAGCGGC AAATGGTICA GAAGTCGT CAGTGGCGGG CATGCCAC TGGCGGAAGA GG AGCAAGAAAT

GA

TGCATTATAG

TG

GCAAGA ITCA AG TACCATAC

GI

ATGGACAACA

GC

CAATCACGAG

TTI

ACTCCTCTGT

TC

GTAAAACAAC TG ATTATTTTAC AC ATCCTGATGG A;~ TTAACTGGTC GI TI'CAATTACC

T

AAGCdAATGT

C

ATGAGCGA.AT

C~

ATGATTCAGA

TD

T'GCCGCCTGT

A'

ATTTTGATCGA

TIGAAAGATCA

A

CCGTGAAAGT C AACTTAGTCC

A

CCAACAA-AGC G GCGGCGGCGA

T

GGTTAGATCC T kTAGATTACGA

C

A.AACCATTCC

AACCAGAFI'C

3 GAGCAGAACT T1 3 CAATTGACTT ATGGAATTCT

C

ZA'TCAGAAGA 3 ATTATCAAAA G AAAATGCCGT T CGmTOGGTTGT G TAGAAATAAC T ATGCCAAAAC T TAGACGCTAA G ATGAAGCAGG

TTGAITI'AAA

;A CGATTGCCGT .T TAACCAATCA iG ATGGTTCGGT kC CAAGCGAGA.A C'G TGATTGAATT 'A ATACCGCATC IrC AACATGGTGG rG TGTACTGGCA rG ATACACCCTC -G TAACAGAAGA TT ACACACTGGA GG CCCATATI'GA GG GGAGTACAGA TC ATGGGGATGA AG GGACTAAAGG 11AACAGC

CCTTAGCG

CCAATGTG

TGGATGAC

'AGTCTA

:AACGAGCG

CGCCAGCC

:CTCG'ITCG

;AATCGACG

;GTGATGAA

CACCAGCA

MT1CCAGAA 3GCAAT'ITG

LATGGAACC

kCGTCTGAA

~GGCGGGGC

GTGATTCCA

PCGCCCAAT

ACAAATCCA

TATGAGITA

GATGAATAT

TATCGTCTT

GTGCCTTT

'GAACCAACT

'GAAGCCAAT

.AAAGACCAA

=TACA L'TA

'GTGGAAGGA

.TTACATGAT

GAAGGCGAT

rGATGCCACA rTCAACGAT

;ATTACGGAT

CAAAGATACA

rCGTAATGCA

AAGTGATGCC

CAATCCTCCG

AAAcAATCAT

TGCGCAAAAA

TAATTI'AAGT

AACCTArI'TG

TGAA.AAAGTG

AAACCAAAAT

TAAGACGTCT

TTATACAAAC

CGAPATCAGTG

TGTA.ATGATC

ACCAAACCAA

CGGAAcTATT

AGTTACCACC

AGCACCTTAT

CACGGTATCC

CAATGGCGAT

CAAAATTCAG

224 TABLE 1. Nucleotide and Amino Acid Seqeuenccs of E. faecalis Genes.

a a a a

CTGAAGPAAA

GACCAAGCTA

TTTGGTGGGT

ACAGTTTCGG

GAAGGAGCAC

GATGAGAAGG

CCGTTTACTC

TTAGAGGTGG

GGCTATCTTT

ATT~CGTGATG

GACCAAGCAG

GCAGTTCGAG

CCAGGAAAAT

CCAAGTGCTT

GCTAACTTTG

FI'ATTAAGTG

rrGACGACAA

TTTGTAGAAA

ATTGCTGAGA

TACAA.AGGGG

G MIT TGA.AT

GATGGCAAAA

GCACCAAAAT

TTCGTGGTG

AA.AGGACGCG

TTTAA.ATTGT

AAAGATGGTt

GATGCAACGG

TCAAATGATA

GGACGTAAAG

GCCGATGAGC

AAAGTTTCTG

GGGCATTACG

CATCCAT'TTG

ATCAATTATC

GGTGCGGTGI

ATGTCTGAC;

GTGGAGACAC

ATTCCCAAAC

TACCAAGGTC

GAATI'TAAAC

AACCAAGCG'

AAAGCACCGC

GCGAAAGAC)

TCCAAAAAA2

CTTGG'ITA(

TTGGTCGG7

CAGCCATGGA

AAACACAAGT

TGCCACAAGG

ACGAAT1'AGC

AACCAACCAT

GTAAAAAGTT

ATTGGGAAGA

ATAGTTTAAA

TAGACACGAC

TTCATGTCAA

GCAATCCATT

AACACTTAGT

ATCAATTTGT

TCACGATTG.C

ITAACTATCA

GTGCCACA LT

ATAATCAAGG

CCAAAGCGCC

AAAATGCTGG

CTTTCCAAMA

TATATGATCA

TTATCTTTAC

TACCAGATGC

ATTTCAAAGC

CCGTC TITAM

ATCGAATCG;

CATTGGCTA)

ATGGCTATAJ

AACAACCAC'

TCAACGAGC2

AGAATCAGC(

AAATAACAA(

'ITTTAGTGGi

AMMTACCG(

AAGGAACTG,

TTAAGGTCA:

AGCAAGGCA

AAGCGCCAA

ACAACCAAG

CTGCCAAGC

TGACAGATG

U TAG'N'CAAG

ATGGTTACC

k AACCTGAAC k. CAA.AACCAA

:CGAAAACCA

r TGGCAAGTI

TGAGACGACT

CCTACGTGAA

GCAATACATT

TAAAGGCCGA

TATTAAAAAC

AGTCAATGCT

AG ITCCCCTT

ACCAGGGCTT

CCCCAAACCA

AATGCTTAAT

AGCAGGTGCT

TCGGATGCA

GGAAACCAAA

AGCAAGCGAT

AGGCACGGCT

TA.AGTGC'T

GGAAATTGTT

AACAGGCTAT

TAALACCAGCG

CGTGAAAACG

CAATAAACAA

AGACTTGGCG

CGCAGATTAT

TGATCCGGAG

GAAAATTGAT

AAACGGGGAA

GGAGGATTT-D

r CGTCAATAA-P r AGATGAGTTP k. AGGTZ-AAACC

CCAAGGTTCV

GGATAAGAC9] k. AACGAA-AGCI

CCAATTAGGI

TCAATTAAC(

r TGATGAAAC A. AGTCATTGC( C TAGCTATCT G CAAACCAGC( T GGTGAA.AAT' C AGAGACAGG, T GAACCACTT.

A ACTGTCTAA T CGTGAATGC A TCAGCCAAC ,A CACACAAGT 'G GCTCTTCTA ATTT1'AGCAG

GGTACAGTAG

TTGGTGGAGA

GTCATTACTA

GATGTCAATA

CGCTTTAAAT

GCOCCGGATC

TATCAGITCA

TTCATCGTGA

TACCAAGGT

GAATT~TTCAG

AAC'GGAAAAG

GCGCCAGCAG

CGGGGCAAAC

AAATTAATCA

GATGCGAAGG

GCAGACCACT

TTATTrAAATA

GTCGTGGTI'G

AATAGCGCAG

TCATTAGGGA

CCAGGTACC'I

A'ITAT1'TATC ATTTTC CAMq

GCCAATGCGP

AAAATCTTTC

GGTGCTGGT;

GCGCCCA'IT

ATGCCACCGG

CAAAAGCACC

TTC-ATGAAGA

AAGTATrT=

TAGAGCATGC

GAACCAACGC

CAGAAATCGA

CACAAAATAC

CTGCTGAACT

TCCTTGACAC

TCACAGTGAC

GGTACC TTT CAGCAA CACT

AAAAAGATGT

GAGAAACGAT

TAGCC CC AGO

CCACGCCAGT

CTAGTGACAA

ACCAACCATT

TTACAGCAAC

ATTATTACAA

*CTGAATTAGT

*TAGGGGCCIT

ACCCACTTCC

AAAGAGAAGT

LGCTATGAATTI

:CAAATTGG

;GTTATCACA

%GAAGGCTAT

AACTTCAGCC

AGAAAAAATC

CGTAACCACG

GAATGGCCAG

PGCACCGACA

GAGCGGACALA

AATTAAAAAA

CACAGGACAA

GGATTTAGCC

AAACACTGAA

TATAGCAACG

GAATGGACAC

TCAAACAGGC

AAAATATCGC

CCCATTTGAA

CTTTGTGAGT

AGCAGGTGCT

GAGTGGCAAA

AGAAATCAAA

AAAAGTAGAA

CGCCAATTTC

AGGAACGATT

AACTGCTGA

AGATGAACTG

CAACCCATTT ATTGTAGT GAAGAAGAAT

GAGTTTGTA.A

TACGGGTG

CCGATAACAT

GGCGAAATT

CCAACAGGCT

AAAGAGCAGC

AAAGAAAACG

GGGCAAACCG

AAAAACITAG

CTTAATG"-AAA

ACTGTGGTAC

GATCAGCAAA

CAAACTGTCG

CAACCAGGAA

CAAGCTGTCG

GGCACGTITG

ACGAAkACAAG

AATTACT'I'C

AAAAAGAGCA

A ITATCAAGC

CAGTTTTTGC

TCTTGA.ATCG

ACGCTAAAGG

ATCTGTTAGA

CAATTGCTr

AAACAGGTGA

TAGATOGACA

CACCGGGAAC

CGCCAAGCGC

TTAAAGCACC

AGAATGCCTT

CTCGTTCATI'

AATATACCT

CATTCACTAT

TTAACGAGAA

CAGCTAGAGA

FrGTCT'ITAT AGAAkATAA

AGAAGTAATG

AATTTACAAT

TGCAGGAGAA

GCTAAATGAA

CACAACGCTA

AGGCGATCTT

AGCATTGGCA

AACCAATCTG

GTATCGTITT

AAGCTT1'ACG =rTAT'rAAT

AGCAGGTGCT

ACGTTCTGAC

TGTGGAAACA

TGCGGCAACA

ACAACCTGTA

GACAGGTTGG

CGGCCTCATG

a EF123-2 (SEQ ID NO:458) MRXNGPMV NRWLYGLMCL LLVEJNYGTPL MALAEEVNSD GQLTLGEVKQ TSQQEM4TLAL QGKAQPVTQE VVVHYSANVS IKAAHWAAPN NTRKIQVDDQ KKQIQIELNQ QALADTLVLT LNPTATEDVT FSYGQQQRAL TLKTGTDPTE STAITSSPAA SANEGSTEEA STNSSVPRSS EETJASTTKA IESKTTESTT VKPRVAGPTD ISDYFTGDET 225 TABLE 1. Nucleotide and Amino Aci d Seqeuences of E. faecalis Genes.

TIIDNFEDPI

PNKPGSGDLV

GDWVIDI PTQ

VTETWPTGNT

YQTTIDE-AVI

EFTWEINYNY

DYKVVINGDG

SQQNI IKNTG

TGAQLTLGKD

DHYRNTAAID

NRLVDAFLTD

WRVDFPNDSR

KGGEYHKDDP

PDKSVILEEG

QVS ITGNGSE

QAKTQVLREG

GAQPTI IKND

LVDSLKPGLY

QAGNPt.AGAE

SAFTIAASDF

TTNNQGEIVA

KGAFQIVKT'

PKLPDGADYI

KLYRIENGE?

NDKQPLDELI

VS=EITTDKT<

NYQGTAQLTn

ETQAPTSYLI

FKVTDAETG

KDKPELVNA(

YLNPDGTPAT

DAEGNVYTY

EDLPPVVIPI

FKSVKVYELV

PD)GGGDVPFK

GEQTI PKDQA SFAIDFLHDv

AVDYQNSTIG

FMVEITRNAD

WTDEAGNNHH

P1 LTNQTYLA TYVIEF1KTSV

DHVYWHVMIN

KDYTLEVTTO

VVHGDDNGDV

TVDATGVITF

VNKVFLEKMO

QFTEIEAPTG

FSVELDTTGQA

GKPATVIATP

EHLAPGKYRE

I SADQPLAGA% IYPELvKVE]

IFEREVTAEV

FVNYQAEVMC

EIYAKGLNEC

ENETGEALA(

NETPSASFT'

2TVARSLRSDI

STFVNEKQPV

ppyKEDVTIH

TISEDC-TVRF

VPDTEQQIDK

MNLDGTIKEV

NHATTSDN

VITDTMGIDNL

TGAVKIDYKT

WTLAVNQiqDY GETGFKVSF I

SEDSKPFKPL

GSLKVYEGNT

DERVIEGSAS

GAQSVLDDVV

NETGQQKIVV

VVDIDHSGG',

GGLPQGQYIL

EKGKKLVNAR

YLLD'TPKRF

*VREHLVSDM~

NFVNYQGTA.?

*VETKAPTCYI

FELYDHNKQ~l

RGDFKGDPE:

DGSLAMEDL(

RKVNEQGQTI

3 HYVLVETKA:

"AVFKVIDETI

r AKDNQGKPA: .4 QGLVQVNHL S KKTKPNQPT WNFNWS IPED

TFNERITSES

QGHFDRTPNP

GRELSPDEYT

PNGLDAEATV

TFEPDSLHLY

KVDG IVEGDV

LMENAVITOT

GAYA.KTSDAF

PAFDLNAQKS

Y1,PDGSVEKVK YID~rASYTNQ

ITDTPSPNQV

KI4AH IF.APYY

AT-.TKGKIQL

VETKAPEGYT

FKLEHAV~r

IVTQNTSGQI

I GKVTVTDLAE LIKKDVNGH1 LNTTPvPFE] 3 LGITATSGKI

EFQLGAFANFI

3AGsYELDELI L, AGAVFAIYN) P TGYLLDTTLI

SQTVDGQTNLI

T VVLKAPFINT Q PGKYTFVET T KQAARETGW VREQ1MflAGDY F DIIIGDFSLDT H SAITWTVDIN

Q

VDKNGNVTIK

G

TATYCMLDK

R

sVTFDDKGNEV AVNNRVIDVGT G YEPVPGLTMV P HITYTTFFDV

TI

GVYNAVTKEI PTQPLTDITM

I

GSSRDVTG(V

LDPESLVIYG

MEYRSLVTSS

KXTAMDETTI

*VSDELAKGRV

FTHWEEVPLA

*RDVHVKMLNY

GKYQFVETKA

LSGATFKVLO

-AEKNACKPAV

G KIIFRDLAP C GRAVFKY\IDA

ATDCYIVNKQ

k. DEQNQPQGSP A PFDVTAQLGK A SDXQGKVIAK C AAKLVKID K APDGYQLSKQ L GLPKTNTQVN

EFQLPGNLK

LNDSDGRGP

AMKT)(QTNPT

OTNKAYRLE

NIDYDEANQ

VGAELVEGK

*QHSEDDGTA

NSLVVKDTT

'ELDAINNPAL,

7tTIAVNLSN

~EPSEKNQNT

IQHGGESVK

CNVTEDGTIT

%AG STDTVSN

.AGAHFQIWD

ITI DEETSAE

PEDRTNANGQL

QGSAELIKKD

PAGYLLNTEP

AKGETIQTGL

VVASDNFrVSY

GTYYYKEIKA

NANPLPGTIF

P IYFVTKKINS

ITFLNRAGEK

EQPIALGDLI

NLAPG-YRF

QQKNALAGAE

AVAFTIAATA

YFFVF IGLML VGLASWLFYYK

KSKK

EF123-3 (SEQ ID NO:459) *6 GGAAGA GGTTALACAC GATGGCCACT TAACGTTAGG

AGAAGTGAAG

CTTCAAGGAA AAGCACAACC

AGTAACACAA

TCAATCAAAG CTGCACATTG

GGCAGCGCCC

CAGAAGAAAC AGATTCAAAT

TGAATTGAAT

ACGTTGAACC CTACAGCTAC

-ACAAGATCTG

IrTGACGTI'AA AGACTGGTAC

TG.ATCCGACA

GCATCAGCGA ATGAAGGTTC

AACAGAAGAA

TCCCAAGAAA CTGTCGCCAG

CACCACAAAA

ACTGTCAAAC CGCCCGTAGC

AGGACCAACA

ACAACGATTA TCGATAAT'IT

TGAAGATCCG

ACACCGCCGT ATAAAGAAGA

TCGTGACCATT

GATGTGCGAG AACAAATCAA

AGCAGGCGAT

AAACCTAATA AACCAGGTTC

AGGTGATTTA

TACACAATA GTGAAGATCG

TACGGTTCGT

AGTGACATTC ACGGGGACTT

TTCTTTACAT

CCAGGAGATT GGGTGAI'GA

TATTCCTACA

ATTCTCCCAG ATACC';AACA

ACAAATTCMI

CCTACTCCGA TrACTTGGAC

CGTAGATATC

ACTGTGACGG AAACATGGCC

AACAGGGAAM

CAAACCAGCC

GAGr'ITGTAG

AATAATACGC

CAGCAAGCGT

ACGTT'IMTT

GAATCAACG

GCATCTACAA

GCGATAGAAA

GATATCAGTG

ATTTATTTAA

CATTGGAACT

TAcTTCGAGT G'1'GATGCAG ITI'Acc IA AcTcATA

*CAAGAAGATT

*AAACAAGGCC

*AATCAAGCG.A

*ACCTTTAAGIl

AGCAAGAAAT

TCCATTATAC

GcAAGATTCA

TAGCAGATAC

ATGCACAACA

cAATCACGAG

ACTCCTCTGT

GTAAAACAAC

ATTATTMAC

ATCCTGATGG

TrAACTGGTC TrCAATTACC

AAGGCAATGT

ATGAGCGAAT

ATGATTCAGA

TGCCGCCTGT

A~ITTGATCG

TGAAAGATCA

'CCGTGAA.AGT

;ACCTTAGCG

['CC CAATGTG krCTGGATGAC

GTTAGTCTTA

GCAACGAGCG

TTCGCCAGCC

TCCTCGTTCG

TGAATcGACG

AGGTGATGAA

AACACcAGCA

GATTCCAGAA

TGGCAATTTG

CTATGGAACC

CACGTCTGAA

TCCGCGGGGC

ArGCTTCCA

AACGCCCAAT

AACAAATCCA

CTATGPAGTTA

226 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

GTGATGAATC

ACCGTTGATA

GAGTACCAAA

AAAAATCACG

GTTACCGCCA

CAAGAATTCA

GCAGTCAITA

TATTCAGTGA

AAAGATTACA

GTGACTGGCG

GTTGCCGTGA

GCCAOTCAAC

GGTTGGACGT

ACGTACCAAC

ACCACTGGTG

GATGGTGAAA

TTCCACATAA

TTGGACCATT

TTrGATGGAAC

AAAATGGCAA

CGACGA'ITGA

CGACGTTAAC

CATATGGCAA

C'rrGGGAAAT

CAGACACAAT

CATTTGATGA

AAGTGGTAAT

CAGTCAAGAT

ATAATCGTGT

AAAATATTAT

TAGCTGTGAA

CAGTTCCTGG

CTCAGTTGAC

CAGGCTTTAA

CTTATACTAC

ATCGAAATAC

AATTAAAGAA

TGTGACGATT

CGAGGCGGTT

AAGTGATAAT

AATGTTAGAC

TAACTACAAC

GGGGGATPAT

CAAAGGAAAT

CAACGGAGAC

TGATTATAAA

GGATGTTGGC

TAAAAACACT

TCAAAATAAT

CTTAACTATG

GTTAGGCAAG

GGTLAGTT

CTT=TCGAT

CGCTGCCATT

GTGGGTCGCG

AAAGGTGACA

ATTCCAGATG

AATCCAAATG

AAGCGCAATA

TATGGTGAAC

TTAACGTTrG GAAGTCG1TG

GGTTCCTIG

ACCAAAGTIG

ACTGGTCAGC

GC6TGcAG TG TA ITTGATCC

GTACCCAATT

GATTTCATGG

ATAGGGGCGT

GTTACCGCT

GATTGG

AACTTAGTCC

CCAACAAAGC

GCGGCGGCGA

GGTTAGATGC

TAGATTACGA

AAACCATTCC

AACCAGATTC

GAGCAGAACT

CAATTGACTr

ATGG-AATTGT

ATTCAGA-AGA

ATTATCAAAA

A.AAATGCCGT

cGT~&FGT

TAGAAATAAC

ATGCCAAAAC

TAGACGCTAA

AGATGAATAT

GTATCGTCT

T'GTGCCTTTT

TGAAGCAACT

CGAAGCCAAT

AAAAGACCAA

TrI'ACATTTA TrTGGAAGGA

TTTACATGAT

CGAAGGCGAT

TGATGGCACA

TTCAACGATT

GATTACGGAT

cAA.AGATACA

TCGTAATGCA

AAGTGATGCC

CAATCCTGCG

EF123-4 (SEQ ID NO:46O) .s a.

4~ a

EEVNSD

GQLTLGEVKQ TSQQEMTLAL KKQ IQ IELNQ QALADTLVLT SANEGSTEEA STNSSVPRSS TI IDNFEDP I YLNPDGTPAT PNKPGSGDLV DAEGNVYGTY GDWVIDIPTQ EDLPPVVIPI VTET WPTGNT FKSVKVYELV YQTTIDEAVI POGGGDVPFK EFTWE INY'NY GEQTIPKDQA DYKVVINGDG SFAIDFLHDV SQQNI IKNTG AVDYQNSTIG TGAQLTLGKD FMVEITRNAD DHYRNTAAID W

QGKAQPVTQE

LNPTATEDVT

EETVASTTKA

PPYKEDVTIH

TISEDGTVRF

VPDTEQQIDK

MLDGTIKEV

NHATLTSDNN

VITDTMGDNL

Tr.AVKI DYKT

WTLAVNQNNY

GETGFKVSF I

VVVHYSANVS

FSYGQQQRAL

I ESKTTESTT WNFNWS IPED

TFNERITSES

QGHFORTPNPi

GRELSPDEYT

PNGLDAEATV

TFEPOSLHLY

KVDGIVEGDV

LMENAVITDT

GAYAKTSDAF

IKAAHWAAPN

TLKTGTOPTE

VKPRVAGPTD

VREQMKAGDY

DIHGDFSLDT

SAITWTVDIN

VDKNGNVTIK

TATYGKMLDK

SVTFDDKGNE

AVNNRVDVGT

YEPVPCLTMV

H ITY'ITFFOV

NTRKIQVDDQ

STAITSSPAA

ISDYFTGDET

IEFQLPGNLK

HLNDSDGRGP

QAW(DQTNPT

GDTNKAYRLE

RNIDYDEANQ

VVGAELVEGK

GQHSEDDGTA

PNSLVVKDTT

TtLOANNPAL EF124-1 (SEQ ID NO:461) a. a a.

TAAAATAAAA

ATGAAAGAAA

TTGTTACTTG

GATGGCCAGT

CTTCAAGGAA

TCAATCAAAG

CAGAAGAAAC

ACCTTGAACC

TGACGTTAA

GCATCAGCGA

TCCGAAGAAA

ACTGTCAAAC

ACAACGATTA

ACACCGCCGT

AATTGGTACG

TGAGAAAGAA

TTCTAAkATTA

TAACGTTAGG

AAGCACAACC

CTGCACATTG

AGATTCAAAT

CTACAGCTAC

AGACTOCTAC

ATGAAGGTTC

CTGTCGCCAG

CGCGCGTAGC

TCGATAATTT

ATAAAGAAGA

AAGTGAACGT

TGGTCCAATG

TGGCACACCA

AGAAGTGAAG

AGTAACACA

GGCAGCGCCC

TGAATTGAAT

AGAAGATGTG

TGATCCGACA

AACAGAAGAA

CACGACAAAA

AGGACCAACA

TGAAGATCCG

TGTGACCAT

AGCAGGCGAT

TCTCTTCTAT

GTAAACCGTT

CTCATGGCTT

CAAACCAGCC

GAGGTTGTAG

AATAATACGC

CAGCAAGCGT

ACG=CTT

GAATCAACGG

GCATCTACAA

GCGATAGAAA

GATATCAGTG

ATTTATITrAA CA=rGAACT

TACTTCGAGT

GTGTCGTTAG

GGCTCTACGG

TGGCGGAAGA

AGCAAGAAAT

TGCATTATAG

GCAAGA7TCA

TAGCAGATAC

ATGGACAACA

CAATCACGAG

AcTCCTCTGT

GTAAA.ACAAC

ATTATTITAC

ATccTGATGG TrAACTGGTC

TTCAATTACC

TAGAGGAAGG

GTrGATGTGT

GGTTAACAGC

GACCTI'AGCG

TGCCAATGTG

AGTGGATGAC

GTTAGTCTTA

GCAACGAGCG

TrCGCCAGcC

TCCTCGTTCG

TGAATCGACG

AGGTGATGAA

AACACCAGCA

GATTCCAGAA

TrGGCAATTTG GATGTGCGAG AACAAATGAA 227 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

AAACCTAATA

TACACAATTA

AGTGACATTC

CCAGGAGATT

ATTGTCC'CAG

CCTAGTGCGA

ACTGTGACGG

GTGATGAATC

ACCGTTGATA

GAGTACCAAA

AAAAATCACG

GTTACCGCCA

CAAGAATTCA

GCAGTCATTA

TATTCAGTGA

AAAGATTACA

GTGACTGGCG

GTTGCCGTGA

GCCAGTCAAC

GGTTGGACGT

ACGTACGAAC

ACCACTGGTG

GATGGTGAAA

TTCCACATAA

TTGGACCATT

CATTCAGAAC

AGCGGTGTTT

AATAATCGTI

GCTGGGAGC'I

AAACCAACGC

ACTTGGCGTC

GTTGATGAA.

CAAGGTTCT']

AAAA.AAGGTC

AATGGCGCCC

GTGCTAGATC

ACGCCAGATI

GATAATGAAJ

TATATGGAAW

AACCAAGTG'

GTGGTCG?1'(

CTGAAGAAAJL

GACCAAGCTM

TTTGGTGGG'

ACAGTTTCGI

GAAGGAGCA,

GATGAGAAGI

CCGTTTACT

TTAGAGGTC'

GGCTATCT

ATTCGTGAT

GACCAAGCA

GCAGTTCGA

CCAGGAAA.A

AACCACCTTC

GTGAAGATGG

ACGGGGACTT

GGGTGA'I GA

ATACCGAACA

TTACTTGGAC

AAACATGGCC

TTGATGGAAC

AAAATGGCAA

CGACGATTGA

CGACGjTTAAC

CATATGGCAA

CTTGGGAAAT

CAGACACAAT

CATTI'GATGA

AAGTGGTAAT

CAGTCAAGAT

ATAATCGTGT

AAAATA ITAT

TACCTGTGA.A

CAGTTCCTGG

CTCAGTTGAC

CAGGCTTTAA

CTTATACTAC

ATCGAAATAC

ATAGTAAACC

ACAATGCCGT

TAGTCGACGC

TGAAACTCTA

AACCG'ITGAC

TTGATTTTCC

LAAGTTATCGP

CACGTAI

GCGAATACC;

AATCCGTTT1

CCGAGTCAT']

k. AATCTG.TTAJ k. CAGGACAAC) C' ATCGTAGLT' r' CAATTACTG( SACATTIGATCj k. CACCCATGGQ k. AAACACAAG' r' TGCCACAAG( 72 ACGAATTAGI C AACCAACCA' 3 GTAAAAAGT C ATI'CGGAAG.

G ATAGTTTAA T TAGACACGA G TTCATGTCA G GCAATCCAT G AACACTTAG ,T ATCAATTC

AGGTGATI'TA

TACGGTTCGT

TTCTTTAGAT

TATTCCTACA

ACAAAI'TAT

GGTAGATATC

AACAGGGAAT

AATTAAAGAA

TGTGACGATT

CGAGGCGG'T

AAGTGATAAT

AATGTTAGAC

TAACTACAAC

GGGGGATAAT

CAAAGGAAAT

CAACGGAGAC

TGATTATAA-A

GGATGMGGC

TAAAAACAC'I

TCAAAATAAI

CTTAACTATC

GTTAGGCAAG

GGTAAGTTTrI

C'FITITCGAI

CGCTGCCATI

GTTTAAACC9

*CACCAAAGPJ

CTT'TTGACC

TGAAGGCAA'

GGATATCACJ

TAATGATAG

AGGTTCGGC'

GACAGGAAAi

CAAAGATGA'

7AGACGATGTC GGTGA=TAl 7TTTAGAAGA kAAAAATTGT r AGTGACT'C'

AAATGGTTC

k CAGTGGCGG k. TGAGACGAC r CCTACGTGA

GCAATACAT

C TAAAGGCCG r TATTAAAAA r AGTCAATGC A AGI'CCCC]l A ACCAGGCC'I C CCCCAAACC A AATGCTI'AJ T AGCAGCTGC T TTCGGATGC T GCAAACCAJ

GTTGATGCAG

TTTACCITA

ACTCAT7IGA

CAAGAACA'T

AAACAAGCC

AATCAAGCGA

ACCT'FrAAGT

GTGGGTCGCG

AAAGGTGACA

ATTCCAGATG

AATCCAAATG

AA3CGCAATA

TATGGTGAAC

TTA.ACGTTTG

GAAGTCG'TTG

GGTTCCTTTG

ACCAAAGTTG

ACTGGTCAGC

GGTGCAGTTG

TATTTGATG

GTACCCAATrf

GATTTCATGG

ATAGGGCCGrl

GTTACCGAGE

IGATTGGACGC

I TACCTGCTq kATCACTTGGP

'GATCCAATT.

1' ACAAAGCCAC

ATCGAAGAAC

r CGTACGTATC r AGTTATGACI P, GITTCTA'IT r CCAGATCATC

"GTTATTACTX

CGGTACCAAC(

A GGAAAAGAT' C GTTAAAATG T TCAGCGGCG A GAAGTCCTT, G CATGCCACA T ATTTTAGCA A GGTACAGTA T TTGGTGGAG A GTCA 1TACT ,C GATGTCAAT :T CGCmTAAA .r GCGCCGGAI .T TATCAGTI'C ,A TCATCGTC CT TACCAAGGrI "T GATTrrC; "A AACGGAAAM k.A GCGCCAGCI AAGGCAATrGT

ATGAGCGAAT

ATGATTCAGA

TGCCGCCTCGT

ATTTTrCATCG

TGAAAGATCA

CCGTGAAAGT

AACTTAGTCC

CCAACAAAGC

GCGGCGGCGA

GGTTAGATGCC

TAGATI'ACGA

AAACCATTCC

AACCAGAITC

GAGCAGAACT

CAATTGACTT

ATrGGAATTGT ATrCAGAAGA

ATTATCAAAA

AAAATGCCCT

CGTTGGTTCT

TAGA.AATAAC

ATGCCAAAAC

'TAGACGCTAA

ATGAAGCAGG

TTGA~rTAAA

CGA=ECGGT

TAACCA.ATCA

ATGGTTCGGrI

CAAGCGAGAA

TGATTGAATri kATACCGCATC

AACATCGTGC

3TGTACTGGC; 3 ATACACCCTC

STAACAGAAGI

r ACACACTGGI 2CCCATATTGI 2GGAGTACAG) C ATGGGGATG] G GGACTAAAG( G GCGCCCATrL G ATGCCACCG( A CAAAAGCAC, A TTGATGA-AG.

'A AAGTATTT .T TAGAGCATG 'C GAACCAACG A CAGAAATCG ;A CACAAAATA 'T CTGCTGAAC iG TCCTI'GACA 4G TCACAGTGA kG GGTACCIT

CTATGGAACC

CACGTCTGAA

TGGGCGGGGC

AGTGATTCCA

A.ACGCCCAAT

A.ACAAATCCA

CTATGAGTTA

AGATGAATAT

GTATCCGrCTT

TGTGCCTTTT

TGAACCAACT

CGAAGCCAAT

AAAAGACCAA

TTTACATTTA

TGTGGAAGGA

TTTACATGAT

CGAGCCAT

TGATGGCACA

TTCA.ACGATT

GATTACGGAT

CAAAGATACA

TCGTAATGCA

AAGTGATGCC

CAATCCTGCG

AAACAATCAT

.TGCGCAAAAA

*TAATTTAACT

AACCTATTTG

TGAAAAAGTG

*AAACCAAAAT

*TAAGACGTCT

TTATACAAAC

CGAATCAGTG

TGTAATGATC

ACCAAACCAA

CGGAACTATT

k AGTTACCACC k. AGCACCTTAT k CACGGTATCC k. CAATGGCGAT

CAAAATTCAG

r ccAAATTTGG 2 GT1ATCACA CAGAAG~cTAT h. AACTTCAGCC r AGAAAAAATG C CGTAACCACG C GAATGGCCAG A AGCACCGACA C GAGCGGACA T AATTAAAAAA .C CACAGGACAA .C GGA'ITTAGCC T AAACACTGAA

S

228 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

CCAAGTGCCFI

GCTAACTTTG

TTATTAAGTG

TTCACGACA-A

ITTGTAGAAA

ATTGCTGAGA

TACAAAGGGG

GTTTTTGAAT

GATGGCAAAA

GCACCAAAAT

ATTCGTGGTG

AAAGGACGCG

TTTAAATI'CT

kAAGATGGTT

GATGCAACGG

TCAA.ATGATA

GGACGTAAAG

GCCGATGAGC

AAAGI'TCTG,

GGGCATTACG

CATCCATTTG

ATCAATTATC

GGTGCGGTGI

ATGTCTGACP

GTGGAGACAC

ATTGCCAAAG-

TACCAAGGTC

AACCAAGG

AAAGCACCGC

GCGAAAGACI

TCCAAAAAA2 CTGGT7TA( TTGGTCGGI7 TCACGATT GC

TTAACTATCA

GTGCGACATT

ATAATCAAGG

CCAA.AGCGCC

AAAATGCTGG

CT'TTCCAALAT

TATATGATCA

TTATCTTTAG

TACCAGATGG

ATT'rCAAAG

CCGTCTTTAA

ATCGAATCGA

CATTGGCTAT

ATGGCTATAT

AACAACCACT

TCAACGAGCA

AGAATCAGCC

AAATAACAAC

TTTTAGTGGA

ATGTAACCGC

AAGGAACTGC

TTAAGGTCAT

AGCAAGGCAA

AAGCGCCAAC

ACAACCAAGC

CTGCCA.AGCI

TGACAGATGC

TAGTTCAAC9

ATGGTI'ACCI

SAACCTGAACJ

k. CAAAACCA-ki 'CGA.AAACCA2 r' TGGCAAG=(

AGCAACAT

AGGCACGGCT

TAAAGTZCTT

CCAAATT1'CTT

AACAGGCTAT

TAAACCAGCG

CCTGAAAACG

CAATAAACAA

AGACTTGGCG-

CCCAGATTAT

TGATCCGGAG

GAAAATTGAT

AAACGGGGAA

GGAGGATTTA

CGTCAATAAA

AGATGAGTTA

AGGTCAAACC

CCAAGGT1'CA

GGATAAGACT

AACGAAAGC.A

CCAATTAGG-A

TCAATTAACC

*TGATGAAAC.P

*AGTCATTGCCG

TAGCTATCTJ

CAAACCAGCC

GGTGAAAAT'J

AGAGACAGGC

GAP.CCACTTI

ACTGTCTA-AC

7 CGTGAATGCC k TCAGCCAAC k. CACACAAGT(

GCTCTTCTA!

CGGGGCAAAC

AAATTAATCA

GATGCGAAGG

GCAGAGCACT

TTATTAAATA

GTCGTGGTTG

AATAGCGCAG

TCATTIAGGGA

CCAGGTACCT

ATTATTTATC

A'ITT'CCAAT

G6CCAATGCGA

AAAATCTTTG

GGTGCTGGTA

CAACCCATTT

GAGTTTGTAA

TTAGCGGGTG

CCGATAACA7 GGCGAAA TT7

CCAACAGGC'I

AAAGAGCAGC

AAAGAAAACC

GGGCAAACCC

AAAAACTTAC

CTTAATGAAN

ACTGTGGTA(

7GATCAGCAAJ

CAAACTGTC(

k. CAACCAGGAj

CAAGCTGTC(

3 GGCACGTT( k. ACGAAACAA(

AATTACTTC'

r AAA.AAGAGC.

CAGCAACAGT

AAAA-AGATGT

GAGAAACGAT

TAGCCCCAGG

CCACGCCAGT

CTAGTGACAA

ACCAACCATT

TTACAGCAAC

ATTATTACAA

CTGAATTAGT

TAGGGGCCTT

ACCCACTTCC

AAAGAGAAGT

GCTAT AATT

ATTTTGTAGT

A'IWATCAAGC

CAG'TTrTGC

TCT-A-ATCG

ACGCTAAAGG

ATCTSTTAGA

CAA'FI'CTT

IAAACAGGTGP

TAGATGGAC;

CACCCGGGAAC

CGCCAAGCGC

TTAAAGCAC(

k. AGAATGCCT' 3CTCGTTCAT'.

k. AATATACCT'

SCATTCACTAK

T TAACGAGA, 3 CAGCTAGAG, r TTGTCTTA' A. AGAALATAA

TATAGCAACG

GAATGGACAC

TCAAACAGGC

AAAATATCGC

CCCATTTGAA

CTTGTGAGT

AGCAGGTGCT

GAGTGGCAAA

AGAAATCAAA

AAA.AGTAGAA

CCC AAT=~C

AGGAACGATT

AACTGCTGAA

AGATCAACTG

GAAGAAGAAT

AGAAGTAATG

AATTTACAAT

TGCAGCAGAA

CTAAATGAA

*CACAACGCTA

*AGCGATCTT

AGCATTGGCA

LAACCAATCTG

GTATCGTT

AAGCTTACG

TTTTATTA-AT

p' AGCAGGTGCT

ACCTICTGAC

r' TGTGGAAACA r TCGCAACA N. ACAACCTGTA P. GACAGGTI'GG r CGGCCTCATG a.

EF124-2 (SEQ ID NO:462) MRKNGPMV NRWLYGLMCL LLVLNYGTPL MALAEEVNSD

GQLTLGEVKQ

KKQIQIELNQ

SANEGSTEE-A

TI IDNFEDPI

PNKPGSGDLV

GDWVIDIPTQ

VTETWPTGNT

YQTTIDEAVI

EFTWEINYNY

DYKlVVINGDG

SQQNIIKNTG

TCAQLTLGKD

DHYRNTAAID

NRLVDAFLTD

WRVDFPNDSR

KGGEYHKDDP

TSQQEMTLAL

QALADTLVLT

STNSSVPRSS

YLNPDGTPAT

DAEGNVYGTY

EDLPPVVIP I

FKSVKVYELV

PDGGGDVPFK

GEQTIPKDQA

SFAI DFLHDV

AVDYQNSTIG

FM'JEITRNAD

WTDE-ACNNHH

P ILTNQTYLA

TYVIEFKTSV

DHVYWHVMIN

QCKAQPVTQE,

LNPTATEDVT

EETVASTTKA

PPYKEDVTIH

TISEDGTVRF

VPDTEQQI OK

MNLDGTIKEV

NHATIJTSDNN

VITDTMGDNL

TGAVKIDYKT

WTLAVNQNtNY GETGFKVSF I

SEDSXPFKPL

GSLKVYEGNT

DEKVIEGSAS

1GAQSVLDDVV

VVVHYSANVS

FSYGQQQRAL

IESKTTESTT

WNFNWSI PED

TFNERITSES

QGHFDRTPNP

GRELSPDEYT

PNGLDAP-ATV

TFEPDSLHLY

KVDGIVEGDV

LMENAVITDT

GAYAKTSDAF

PAFDLNAQKS

KPDGSVEKVK

YDNTIASYTNQ

ITDTPSPNQV

IKAAHWAAPN

TLKTGTDPTE

VKPRVAGPTD)

VREQMKAGDY

TJIHGDFSLDT

SAITWIVD IN

VDKNGNV'IK

TATYGKMLDK

SVTFDDKGNE

AVNNRVD)VGT

YEPVPGLTMV

HITYTTFFDV

GVYN4AVTKE I

PTQPLTDITM

GSSRDVTGYKV

LDPESLVIYG

NTRKIQVDDQ

STAITSSPAA

ISDYFTGDET

FEFQLPGNLK

HLNDSDGRGP

QAmKD)QTNPT

GDTNKAYRLE

RNI DYDEANQ

VVGAELVEGK

GQHSEDDGTA

PNSLVVKDTT

TELDANNPAL

TWTIAVNLSN

EEPSEKNQNT

S IQHGGESVK

TNVTEDGTIT

229 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

PDKSVILEEG

QVS ITGNGSE

QAKTQVLREG

GAQPTI IKND

EVDSLKPGLY

QAGNPLAGAE

SAFTIAASOR

TTNNQGEIVA

KGAFQIVKTN

PKLPDGADYI

KLYRIENGEK

NDKQPLDELE

VSE ITTDKTG

NYQGTAQLTK

ETQAPTSYLL

FKVTDAETGQ

KDKPELVNAG

KDYTLE:VT'D

VVHGDDNGDV

TVDATGVITF

VNKVFLEKMD

QFTEIEAPTG-

FSVLDTTGQA

GKPATVIATA

EHLAPGKYRF

SADQPLAGAV

IYPELVKVEI

I FEREVTAEK

FVNYQALVMG

EIYAKGLNEG

EN=TEALAG

NETPSASFTI

TVARSLRSDN

TFVNEKQPVS

NETGQQKIVV WKI4MIAPYY VVDIDHSGGH AT4-TKGKIQL GGLPQGQYIL VETKAPEGYT EKG~aLVNAR

FKLEHAVTTP

YLLIDTI'PKRF IVTQNTSr-Q I VRE-{LVSDAN GKVTVTDLAP NFVNYQGTAK LIKKDVNGHL VETKAPTGYL LNTTPVPFE I FELYDHNKQS LG ITATSGKD RGDFKGDPEI FQLGAFANFI< DGSLAMEDLG

AGSYELDELD

RKXVNEQGQTL AG3AVPAIYNA HYWLVETKAP

TGYLLD-.TLH

AVFKV7DET

QTVIDGQT.NLM

AYKDNQGKPAT VVLKAPFINY QGLVQVNH-LQ PGKYTFVETK KI(TKPNQPTT KQAARETGWL MEYRSLNTSS AAGSTDTVSN

KXTAMDETTI

VSDELA1(GRV FTH WEEVP LA RDvWvKMILNY

GKYQFVETKA

LSGATFKVLD

AEKNAGKPAV

GKI IFRDLAP

GRAVFKKIDA

ATDGY IVNKQ

IJEQNQPQGSP

P FDVTAQLGK SDKQdKVIAI(

QGAAICLVKID

APDGYQLSKQ

ITIDEETSAE

PDRTNANGQL

QGSAEL IKKIJ

PAGYLLNTEP

A.KGE.TIQTGL

VVASDNFVSY

GTYYYKE IKA NAN PL PGT IF P IYFVVKXNS

ITFLNRAG:EK

EQP IALGDLI

NLAPGTYRF-V

QQKNALAGAE

AVAFTIAATA

GLPKTNTQVN YFFVFIGLML VGLASWLFYK KSK( EF124-3 (SEQ ID NO:463) TGCCTTCCACATAACTTATACTACCTTT=GACG GATCCAA=I TAACCAATCA AACCTATTTG GCTGGGAGCT TGAAAGTCTA TGAAGGCAAT ACAAAGCCAG ATrGGTCGGT TGAAAAAGTG AAACCAACGC AACCGTTGAC GGATATCACA ATGGAAGAAC CAAGCGAGAA

AAACCAAAAT

ACTTGGCGTG TTGATTTTCC TAATGATAGT CGTACGTATG TGATI'GAATT TAAGACGTCT GTTGATGAAA AAGTTATCGA AGGTTCGG-CT AGTTATGACA ATACCGCATC TTATACAAAC CAAGGTTCTT CACGTGATGT GACAGGAAAA GTTTCTATTC AACATGGTGG CGAATCAGTG

AAAAAAGGTG

AATGGCGCCC

GTGCTAGATC

ACGCCAGATA

GATAATGAAA

TATATGGAAT

AACCAAGTGT

CTrGGTCGTTG

CTGAAGAAAA

GACCAAGCTA

TTTGGTGGGT

ACAGTTTCGG

GAAGGAGCAC

GATGAGAAGG

CCGTTTACTC

TTAGAGGTG

GGCTATCT

ATTCGTGATG

GACCAAGCAG

GCAGTTCGAG

CCAGGAAAAT

CCAAGTGCTT

GCTAACTTTG

TTATTAAGTG

TTGACGACAA

GCGAATACCA CA A~ATCGGTTI AG.

CCGAGTCA IT GG 1ATCTG ITAT TT CAGGACAACA A ATCGTAGTr AC CAA ITACTGG A ACATTGATCA CA CAGCCATCCA TO AAACACAAGT

CC

TGCCACAAGG GC ACCAATTAGC T AACCAACCAT T CTAAAAAGTT Ac ATTCGCAAGA AC ATAGTTTAAA

AC

TAGACACGAC C( TTCATCTCA.A A2 GCAATCCA IT AC AACACTTAGT

F

ATCAATITCGT G( TCACGATTCC AX TTAACTATCA A( CTGCCACATT T ATAATCAACC G k.AGATGAT

ACGATGTG

I'GATTTAC

TAGAAGAA

AAA'ITGTC

TGACTTCT

ATGGTTCA

.GTGGCGG

,ACACGACT

TACGTGAA

.AATACATT

,AAGCCCGA

LrrAAAAAC

,TCAATGCT

TTrCCCC'Fr

.CAGCGC'T

CCAAACGA

~TGCTTAAT

;CAGGTGCT

rCGGATGCA

.,AAACCAAA

~CAAGCCAT

,GCACGGCT

k.AAGTrGCTT CCAGATCATG I GTTA NTACTG CGTACCAACG 9 GGAAAAGATT I

GTTAAAATGGC

TCAGCCCCGGC

GAAGTCGTTC

CATGCCACAG

ATTT'TAGCAG

GCTACAGTAG

TTGGTGGAA

GTCATTACTA

GATCTCAATA

CGCTrAA.AT

GCGCCGGATC

TATCAGTTCA

TTCAT'CGTGA

TACCAAGGTT

GAArF1TTCAG AAcrGGAAAAG

CCGCCAGCAG

CCCGGCAAAC

AAATTAATCA

GATGcGAAGG ~TACACCCTC

EAACACAAGAC

~CACACTGGA

:CCATATTGA

GCAGTACAGA

kTGCCATCA

GGACTAAAGG

GCGCCCA'TTT

WICCCACCGC

CAAAAGCACC

11'GATGAAGA

AAGTATTTT

TAGAGCATGC

GAACCAACGC

CACAAATCOA

CACAAAATAC

CTGCTGAACT

TCC1TCACAC

TCACACTGAC

GGTACCTII'

CAGCAACAGT

AAAAACATC-T

GAGAAACGAT

.CCAAACCAA

:GGAACTATT

kGTTACCACC

%.GCACCTTAT

CACGCTATCC

CAATGCAT

cAAAATTCAG :cAAAT'FrGG

GG'ITATCACA

AGAAGGCTAT

AACTTCAGCC

ACAA.AAAATG

CGTAACCACC

GAATGGCCAG

AGGACCOACA

GAGCGGACAA

AATTAAAAAA

CACAGCACAA

GCAT'ITAGCC

AAACACTGAA

TATACCAACG

GAATGCACAC

TCAAACAGGC

4**b EF124-4 (SEQ ID NO:464) 230 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

AF HITYTTFFDV TELDANNPAL

DHYRNTAAID

NRE.VDAFLTD

WRVDFPNDSR

KGGEYHKDOP

PDKSVILEEG

QVS ITGNGSE

QAXTQVLREG

GAQPTI IKND

EVDSLKPGLY

QAGNPLAGAE

SAFTIAASDR

TTNNQG

WTDEAGNNI4 P ILTNQTYLA

TYVIEFKTSV

DHVYWHVMIN

KDYTLEVTTD

VVHGDDNGDV

TVDAT GVITF

VNKVFLEKMD

QFTEIEAPTG

FSVLDTTGQA

GKPATVIATA

SEDSKPFKPL

GSLKVYECNT

DEKVI EGSAS

GAQSVLDDVV

NETGQQKIVV

VVDIDHSGGH

GGLPQGQY IL

EKGKKLVNAR

YLLDTTPKRF

VREHLVSDAN

N~FVNYQCTAK

PAFDLNAQKS

KPDGSVEKVK

YDNTASYTNQ

ITDTPSPNQV

KMAHI EAPYY

ATGTKGKIQL

VETKAPEGYT

FKLEHAV'ITP

IVTQNTSGQ I

-CKV-TVTDLAP

LIKKDVNGHL

GVYNAVTKE I

PTQPLTDITM

GSSRDVTGKV

LDPESLVIYG

MEYRSLVTSS

KKTAMDEITI

VSDELAKGRV

FTHWEEVPLA

RDVHVKMLNY

GKYQFVETKA

ISGATFKVLD

TWTIAVNLSN

EEPSEKNQNT

SI QHGGESVK

TNVTEDGTIT

AAGSTDTVSN

LAGAHFQ IWD

ITIDEETSAE

PDRTNANGQL

QGSAELIKKD

PAGYLLNTEP

AKGETIQTGL

EF125-1 (SEQ ID NO:465)

TAAAATAAAA

ATGAAAGAAA

T'rGTTAC PTG

GATGGCCAGT

CTTCAAGGAA

TCAATCAAAG

CAGAAGAAAC

ACGTTGAACC

ITGACGTTAA

GCATCAGCGA

TCCGAAGAAA

ACTGTCAAAC

ACAACGATTA

ACACCGCCGT

GATGTGCGAG

AAACCTAATA

TACACAA ITA

AGTGACATTC

CCAGGAGATT

ATTCTCCCAG

CCTAGTGCGA

ACTGTGACGG

GTGATCAATC

ACCGTTGATA

GAGTACCAAA

AAAAATCACG

GTTACCGCCA

CAAGAATTCA

GCAGTCATTA

TATTCAGTGA

AAAGA ITACA

GTGACTGGCG

GTTCCCTGA

GCCAGTCAAC

GGTTGGACG'I

ACGTACGAAC

AAI ICGTACC

TGAGAAAGAA

TTCTAAAT-TA

TAACGTTAGG

AAGCACAACC

CTGCACAITG

AGATTCAAAT

CTACAGCTAC

AGACTGGTAC

ATGAAGGTTC

CTGTCGCCAG

CGCGCGTAGC

TCGATAATTT

ATAAAGAAGA

AACAAATGAA

AACCAGGTTC

GTGAAGATGG

ACGGGGACTT

GGGTGATTGA

ATACCGAACA

TTACTTGGAC

AAACATGGCC

TIGATGGAAC

AAAATGGCAA

CGACGATTGA

CGACGTTAAC

CATATGGCAA

CTTGGGAAAT

CAGACACAAT

CATTTGATGA

*AAGTGGTAA7 CAGTCAAGA7

*ATAATCGTG'I

*AAAATATTAI

TAGCTGTGAP

CAGTTCCTGC

AAGTGAACGT

TGGTCCAATG

TGGCACACCA

AGAAGTGAAG

AGTAACACAA

GGCAGCGCCC

TGAATTGAAT

AGAAGATCTG

TGATCCGACA

AACAGAAGAA

CACGACAAAA

AGGACCAACA

TGAAGATCCG

TGTGACCAT

AGCAGCAT

AGGTGA I TA

TACGGTTCGT

TTCTTAGAT

TATTCCTACA

ACAAATGAT

GGTAGATATC

AACAGGGAAT

AATTAAAGAA

TCTGACGATT

CGAGGCGGTT

AAGTGATAAT

AATCTTAGAC

*TAACTACAAC

GGGGGATAAI

CAA.ACGAAA I

*CAACGGAGAC

*TGATTATAAP

GGATTGGC

*TAAAAACACIl

LTCAAAATAAI

;CTTA.ACTATC

TCTCTTCTAT

GTAAACCGTT

CTCATGGCTT

CAAACCAGCC

GAGGTTGTAG

AATAATACGC

CAGCAAGCGT

ACTrCTT

GAATCAACCG

GCATCTACA.A

GCGATAGAAA

GATATCACTG

ATTTAmTAA

CATTGGAACT

TACTTCGAGT

GTI'GATGCAG

?TTTACCTTTA

ACTCATTTGA

CAAGAAGAT

AAACAAGGCC

AATCAAGCGA

ACCTI'TA.AGT

GTGGGTCGCG

AAAGGTGACA

AT.TCCAGATG

AATCCAAATG

AAGCGCAATA

TATGGTGAAC

TTAACGI"MT

GAAGTCGTT'G

GGTTCCTTTG-

ACCAAAGTTCG

ACTGGTCAGC

GGTGCAG=I2 TATTTGATGc:

GTGTCGTTAGJ

GCCTCTACGG C TGGCGGAAGA C

AGCAAGAAAT

TGCATTATAG

GCAAGATTCA

TAGCAGATAC

AT'GGACAACA

CAATCACGAG

ACTCCTCTGT

GTAAAACAAC

ATTATTITAC

ATCCTGATGG

TTAACTGGTC

TTCAATTACC

AAGGCAATGT

ATGAGCGAAT

ATGATTCAGA

TGCCCCTGT

A'=TATCG

TGAAAGATCA

CCGTGAAAGT

AACITAGTCC

CCAACAAAGC

GCGGCGGCGA

GGTTAGATGC

TAGATTACGA

AAACCAITCC

AACCAGATTC

GAGCAGAACT

CAATTGAcTT

ATGGAATT

A'rrCAGAAGA

ATTATCAAAA

AAALATGCCGT

~'AGAGGAAGG

'ITGATGTGT

GTTAACAGC

;ACCTTAGCG

L'GCCAATGTG

kGTGCATGAC

;-TTAGTCTTA

GCAACGAGCG

TTCGCCAGCC

TCCTCGTTCG

TGAATCGACG

AiGGTGATGMA

PIACACCAGCA

GATTCCAGAA

TGGCAATTTG

CTATGGAACC

CACGTCTGAA

TGGGCGGGGC

AGTGATTCCA

AACGCCCAAT

AACAAATCCA

CTATGAGTTA

AGATGAATAT

GTATCGTC 1T TGTGCCTTrT

TGAAGCAACT

CGAAGCCAAT

AAAAGACCAA

TTTAcATI'TA

TGTGGAAGGA

TrTACATGAT

CGA-AGGCGAT

TGATGGcACA TrCAACGATr

GATTACGGAT

GTACCCAATT CGTTGGTTGT CAAAGATACA 231 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

*5 ACCACTGCTG CT GATGGTGAAA C TTCCACATAA Cl TTGGACCATT Al CATTCACAAC Al AGCCGTCTTT A( AATAATCGTrTI GCTCCCACCT TC

AAACCAACCA

ACTTGGCGTG T GITGATGAAA A CAAGGTTCTT

C.

AAAAAAGGTG Gi AATGGCGCCC A G'IGCTAGATC

C

ACGCCAGATAA

GATAATGAAA C TATATGAAT A AACCAAGTGT C GTGGTCGTTG A CTGAAGAAAA C

CACCAACCTA

TT1'GGTGGGT1 ACAGTTTCGG A

CAAGGAGCAC

GATGAGAAGC C CCGTTTACTC

TTAGAGGTGG

GGCTATC=I1

ATTCGTGATG'

CACCAAGCAG

GCAGTTCGAG

CCAGGAAAAT

CCAAGTCCT'

GCTAACTTC

TTATTAAGTG

TTGACGACAA.

TTI'GTAGAAA

ATTCCTGAGA

TACAAAGGGG

G TTTCAAT

GATGGCAAAA

CCACCAAAAT

ATTrCGTG4GTG

AA.AGGACGCG

TTrAAATTGT

AAAGATGCTT

GATGCAACG

TCAAATGATA

GGACGTAAAG

GCCGATGAGC

AAAG TTCTG

GGGCATI'ACG

CATCCATT'G

~CAGTTGAC CT CGCTAA GG 'TATACTAC CT ~CGAAATAC CG ~AGTAAACC CG :AATGCCGT CA kGTCGACGC CT .AAAGTCTA TC ~CCGTTCAC CC rGATTTTCC T kGTTATCGA AC A.CGTG.ATrCT GI CAATACCA Cl ATCGGTTITT AC CGAGTCATT CG

ATCTCTTAT

AGGACAACA A TCCTAGTTT- AN

AATTACTGC

.CATTGATCA C.

ACCCATGGA T AACACAACT C 'CCCACAAGG G CGAATTACC

T

LACCAACCAT

;TAAAAAGTTA

OTTGGGAAGA A

TAGTTTAAA

PAGACACGAC C

['TCATGTCAA

3CAATCCATT

%.ACACTTAGT

kTCAATTTGT

C

rCACGArrC

ITAACTATCA

GTGCGACATI'

ALTAATCAAGG

CCAAACCC

AAA.ATCCTG

CTTTCCAAAT

TATATGATCA

TTATCmTAG

TACCAGATG

ATTTCAAAG

CCGTCTTrA.A

ATCGAATCCA

CATTrGGCTAT

ATGGCTATAT

AACAACCACT

TCAACGAGCA

AGAATCAGCC

AAATAACAAC

TI-rTAGTCGA

ATGTAACCGC

TAGGCAAG GA TAAGTI'IT AT 'TTTTCCAT CT CTGCCATT GA -MAAACCT T CCAAAGAA ATl -rr'=ACG GA ~AAGGCAAT AC ~ATATCACA Al LATGATAGT CC ;GTTCCCCT AC ~CAGGAAAA Gl kkAAGATGAT CC ;ACGATGTG G~ MTATTTAC G( I'rAGAAGAA CG kAAATTGTC G~ ~TGACTTCT Ti AATGCTTCA CG IGTGCCCG C

GAGACGACTA

CTACGTGAA C

CAATACATTT

AA.AGGCCGA C 'ATI'AAAAAC

G

,GTCAATGCT

C

,CTTCCCCTT G

,CCAGCCCTT''

CCCAAACCA

LATGCCTAAT

=CAGGTGCT C n'CGGATGCA GAAACCA.AA C kGCAAGCGATC

%GGCACGGCT

rAAAGTccTT 3GAAATTGTT

AACACCCTAT

TAAACCAGCG

CTGAAAACC

CAATAAACAA

AGACTTIGCC

CGCACATI'AT

TCATCCCGAG

GAAAATI'GAT

AAACGGGGAA

CGAGGATTI'A

CGTCAATAAA

AGATGAGITA

AGTCAA.ACC

CCAAGCITCA

GGATAAGACT

AACGAAAGCA

CCAATTAGCA

TTTCATC.G T-AL ACGGCGT AT( 'TACCCACT TAI .TTCGACCG AT ,ACCTGc ET TT CACT1'GGA CC ~TCCAATTT TA .AAAGCCAG ATl 'GGA-AGAAC CA ,TACGTATG TG ~TTATGACA Al rTC.TAIC AA .AGATCATG TC rTATTACTG Al 3TACCAACC T .,AAAACA ET AC TTAAAATCG CC CAGCGGCCG CC AACTCCTTrC A' ATGCCACAC G( 'ITTTAGCAG G CTACACTAC A TGGTCGACA C.

TCATTACTA T ,ATGTCAATA A

.CCT'TAMATT

,CGCCCGATC C .'ATCAGTTCA C TCATCGTGA C .ACCAAGGTT

C

.AATTTTAG TI ACGGAAAAG Ti .CCCCACCAC C .,GGGGCAAAC C

"TAIATCA

GATCAAGGC

.CAGACCACT

TTATTAAATA

.TCGTGGTTG

A.ATAGCGCAG

TCATTAGGGA

CCACGTACCT

ATTATTTATC

ATTTTCCAAT

CCAATGCCA

AAAATCTTTG

GGTGCTGCTA

CAACCCATTT

GAGTTICTAA

TTAGCGGGTG

CCGATAACAT

GCGAAAT

cCA.ACACCCT

AAAGACCAGC

~AAATAAC TC( ~CCAAAAC AA' ~ACCCTAA CA.

CAACCAGC AA GATTTAAA TC ATTCCGGT TA ACCAATCA AA GCTICCGT TC ACCACAA AA ATTCAATT TA ,'ACCCCATC TT LCATGGTCG CG TACTGGCA TC 'ACACCCTC AC kACAbAAGA CC :ACACTCCA AC :CATATTCA AC ;AGTACACA CI I'CCGGATCA C~ GACTAAC Cj CCCCATTT C( TCCCACCGG CG AAAACCACC A' TGATGAAGA A AGTATTTTT A ACACCATCC C AACCAACGC C

AGAAATCCAA

ACAAAATAC C TcTGAACTA 'CCTTCACAC C ICACACTOAC

C

;CTACC'TT

ACAACACT I LAAAAGATOT C ;ACAAACGAT 9

[AGCCCCAGC

:CACGCCACTC

CTAGTCACAAC

ACCAACCATT

TIACAGCAAC

ATI'ATI TCAA

CTGAATTAGT

TAGGGCCCTI

ACCCAC'ITCC

AAAGACAAGT

GCTATGAA1T AT7'GTACT

ATI'ATCAAC

CAGTTTTTC

TCTTGAATCG

ACCCTAAAG

ATCTGTTACA

CAATGMC=T

GTAATGCA

GTGATGCC

ATCCTC

ACAATCAT

CCAAAAA

ATTTA-ACT

.CCTATTTC

AAAAAGTG

ACCAAAAT

,ACACCTCT

'ATACAAAC

;AATCAGTC

TAATCATC

:CAAACCAA

;CAACTATT

;TTACCACC

;CACCTTAT

kCGGTATCC kIATCGCAT

AAAATTCAG

cAAATTTGG

GTTATCACA

~AACCCTAT

ACTTCAGCC

GAAAAAATG

CTAACCACG

AATGGCCAG

.GCACCGACA

AGCGGACAA

ATTAAAAAA

ACAGGACAA

GATTTACC

LAACACTCAA

~ATAGCAACC

;AATGCACAC

'cAAACAGC

L.ATATCGC

:CCATTTGAA

:TTTCACT

AGCAGCTGCT

GAGTGCCAAA

ACAAATCAAA

AAAAGTAGAA

CGCCAAT'ITC

AGAACGATT

AACTCCTGA-A

AGATCAACTG

GAAGAAGAAT

AGAACTAATG

AATTTACAAT

TGCAGCAGAA

GCTAAATGAA

CACAACGCTA

AGCATcTT

S

232 TABLE 1. Nucleotidc and Amino Acid Seqeuences of E. faecalis Genes.

ATCAATTATC

GGTGCGGTGT

ATGTCTGACA

GTOGAGACAC

ATTGCCAAAG

TACCAAGGTG

GAA ITTAAAG

AACCAAGGGT

AAAGCACCGG

GCGAAAGACA

TCCAAAAAAA

CTTGGLTIAC

TTGGTCGGTT

KAGGAACTGC

TTAAGGTCAT

AGCAAGGCAA

AAGCGCCAAC

ACAACCAAGG

CTGCCAAGCT

TGACAGATGC

TAGTTCAAGT

ATGGTTACCA

AACCTGAACT

CAAAACCAAA

CGA.AAACCAA

TGGCAAGTTG

TCAATTAACC

TGATGAAACA

AGTCATTGCG

TAGCTATCTT

CAAACCAGCC

GGTGA.AAAT

AGAGACAGGG

GAACCACTTA

ACTGTCTAAG

CGTGA.ATGCG

TCACCCAACA

CACACAAGTC

GCTCTTCTAT

AAAGAAAACG

GGGCAAACCC

AAAAACTTAG

CTTAATGAAA

ACTGTGGTAC

GATCAGCAAA

CAAACTGTCG

CAACCAGGAA

CAAGCTGTCG

GGCACGTrL'G

ACGAAACA.AG

jATTACTTCT

AAAAAGAGCA

AAACAGGTGA

TAGATGGACA

CACCGGGAAC

CGCCAAGCGC

TAAAGCACC

AGAATGCCT

CTCGTTCATT

AATATACCTT

CATTCACTAT

TTAACGAGAA

CAGCTAGAGA

TTGTCTTTAT

AGAAkATAA

AGCATTGGCA

AACCAATCTC

GTATCGTTT

AAGCTTTACG

TTTTAITrAAT

AGCAGGTGCT

ACGTTCTGAC

TGTGGAAACA

TGCGGCAACA

ACAACCTGTA

GACAGGTTGG

CGGCCTCATG

EF125-2 (SEQ ID NO:466) MRKNGPMV NRWLYGLMCL LLVLNYGTPL MA.LAEEVNSD

GQLTLGEVKQ

KKQ IQ IELNQ

SANEGSTEEA

TI IDNFEDPI

PNKPGSGDLV

GDWVIDIPTQ

VTETWPTGN T

YQTTIDEAVI

EFTWEINYNY

DYKVVINGDG

SQQNI IXNTG

TGAQLTLGKD

DHYRNTAAID

NRLVDAFLTD

WRVDFPNDSR

KCGEYHKDDP

PDKSVILEEG

QVS ITGNGSE

QAKTQVEJREG

GAQPTI IXND

EVDSLKPGLY

QAGNPLAGAE

SAFTIAASDR

'rrNNQGEIVA

KGAFQIVKTN

PKLPDGADYI

KLYRI ENGER

NDKQPLDELE

VSEITTDKTC

NYQGTAQLTI(

ETQAPTSYLL

FKVTDAETC

KDKPELVNAC

VGLASWLFY?

TSQQEMTLAL

QALADTLVLT

STNSSVPRSS

YLNPDGTPAT

DAEGNVYGTY

EDLPPVVIPI

FKSVKVVYELV

PDGGGDVPFK

GEQTIPKDQA

SFAIDFLHDV

AVDYQNSTIG

FMVEITRNAD

WTDEAGNNHH

PILTNQTYLA

TYVIEFKTSV

DHVYWHVMIN

KDYTLEVTTD

VVHGDDNGDV

TWDATGVITP

VNKVFLEa<M

QFTEIEAPTC

FSVLDTTGQA

GKPATVIATP.

EHLAPGKYRF

SADQPLAGA%

IYPELVKVE]

IFEREVTAET

FVNYQAEVMC

EIYAXGLNEC

ENETrOEALAC

NETPSASFT:

TVARSLRSDI

TFVNEKQPV!

KSKK

QGKAQPVTQE

LNPTATEDVT

EETVASTTKA

PPYKEDVTIH

TI SEDGTVRF VPDTEQQ IDK MNhLDGTIKEV

NHATLTSDNN

VITDTMGDNL

TCAVKIDYKT

WTLAVNQN.Ny GETGFKVSF I

SEDSKPFKPL

GSLKVYEGNT

DEKVIEGSAS

GAQSVLDDVV

NETGQQKIVV

VVDIDHSGGH

GGLPQGQYIL

EKGK'KLVNAR

YLLDTTPKRF

VREHLVSDAN

NFVNYQGTAK<

VETKAPTGYL

FELYDHNKQS

RGDFKGDPEI

DGSLAMEDLC

;RKVNEQGQT.

;HYVLVETKAI

;AVFKVIDETC

E AKDNQGKPAJ qI QGLVQVNHL< 5 KKTKPNQPT'J

VVVHYSANVS

FSYGQQQRAL

IESKTTES~r WNFNWS I ED

TFNERITSES

QGFFDRTPNP

GRELSPDEYT

PNGLDAE-AT

TFEPDSLHLY

KVDGIVEGDV

LMENAVITC)T

GAYAKTSDAF

PAFDLNAQKS

KPDGSVEKVK

YDN4TASYTNQ

ITDTPSPNQV

KMAH IEAPYY

ATGTKGKIQL

vETKAPECYT

FKLEHAV'ITP

IVTQNTSGQ I

GKVTVTDLAP

*LIKKDVNGHL

LNTTPVPFEI

LGITATSGKE

*FQLGAFANPB

*AGSYELDELE

AGAVFAIYNI

TGYLLDTI'I

QTVDGQTNUl

SVVLKAPFIY.

SPGXYTFVET]

r' KQAARETCGWI

IYKAAHWAAPN

TLKTGTOPTE

VKPRVAGPTD

VREQMKAGDY

DIHGDFSLDT

SAITWT\TDIN

VDKiNGNVTIK

TATYGKMLDK

SVTFDDKGNE

AVNNRVDVGT

YE? VPGLTMV

HITYTTFFDV

GVYNAVTKEI

PTQ PLTD ITM

GSSRDVTGKV

LDPESLVIYG

MEYRSLVTSS

KKTAI4DETTI

VSDELAI(GRV

FTHWEEVPLA

RDVHVKMLNY

GKYQFVETKA

LSGATFKVLD

AEKNAGKPAV

GKIIFRDLAP

GRAVFKYIDA

ATDGYIVNKC

SDEQNQPQGSE

I PFDVTAQLG? d1 SDKQGKVIA? C QGAAKLVKIE k APDGYQLSK(

GLPKTNTQVW

NTRKIQVDDQ

STAITSSPAA

ISDYFTGDET

FEFQLPGNLK

HLNDSDGRGP

QAMKDQTNPT

GDTNKAYRLE

RNIDYDEANQ

VVGAELVEGK

GQHSEDDCTA

PNSLVVKTJTT

TELDANNPAL

TWTIAVNLSN

EEPSEKNQNT

SIQHGGESVK

TNVTEUGTIT

AAGSTDTVSN

LAGAHFQ IWD

ITIDEETSAE

PDRTNANCQL

QGSAELIKKD

PAGYLLNTEP

AKGETIOTGL

WVASDNFVSY

GTYYYKEIKA

NANPLPGTIF

PIYFVVKKNS

ITFLNRAGEK

EQPIALGDLI

NLAPGrfYRFV

QQKNALAGAE

AVAFTIAATA

4 YFFVFICLML

S

a EF125-3 (SEQ ID NO:46 7 233 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

TAATT- TACTATCA AGGCACG'GCT AAATTAATCA AAAAAGATGT GAATGGACAC

TTATTAACTG

TTGACGACAA

TTTGTAGAAA

ATTGCTGAGA

TACAAAGGGG

OTTTTTGMAT

GATGGCAAAA

GCACCAAAAT

ATTCGTGGTG

AAAGC-ACGCG

TTTAAATTGT

AAAGATGGTT

GATGCAACGG

TCAAATGATA

GGACGTAAAG

GCCGATGAGC

AAAGTTTCTG

GGGCATTACG

CATCCATTITG

ATtAATTATC

GGTGCGGTGT

ATGTCTGACA

GTGGAGACAC

ATTGCCAAAC

TACCAAGGTG-

GAATTTAAAC

AACCAAGGGI

AAAGCACCGC

GCGAAAGACI

TCCAAAAAAJ

CTTGGT

GTGCGACA IT

ATAATCAAGG

CCAAAGCGCC

AAAATGCTGG

CTTTCCAAAT

TATATGATCA

TATCTTTAG

TACCAGATGG

ATTTcAAAGG

CCCTCTTTAPA

ATCGAATCGA

CATTGGCTAT

ATGGCTATAT

AACAACCACT

TCA-ACGAGCA

AGAATCAGCC

AAATAACAAC

T'TTrAGTGGP

ATGTAACCGC

AAGGAACTGC

TTAAGGTC'A

AGCAAGGCMJ

AAGCGCCA-AC

ACAACCAAGC

CTGCCAAGCr.

TGACAGATG(

7TAGTTCAAG'

;ATGGTTACC,

k AACCTGAAC k CAAAACCAA

TAAAGTC'I

GGAAATTGTT

AAcAGGCTAT

TAAACCAGCG

CGTGAAAACG

CAATAAACAA

AGACTTGGCG

CGCAGATAT

TGATCCGGAG

GAAA?1'GAT

APLACGGGGAA

GGAGGATTTA

CGTCAATAAA

AGATGAGTTA

AGGTCAAACC

CCAAGGTTCA

GGATAAGACT

AACGAAAGCA

CCAATTAGGA

TCAATTAACC

TGATGAAAC1P

SAGTCATTGCC

TAGCTATCT'I

3CAAACCAGCC r~ GGTGAAAATJ

:AGAGACAGGC

r GAACCACTTJ k. ACTGTCTAA( r CGTGAATGC(

GATGCGAAGG

GCAGAGCACT

TTATTAAATA

GTCGTGGTTG

AATAGCGCAG

TCATTAGGGA

CCAGGTACCT

ATTAI=ATC

ATT='CCAAT

GCCAATGCGA

KZAAATCrG

GGTGCTG;GTA

CAACCCATTT

GAGTTTGTA-A

TTAGCGGGTG

CCGATAACkI

GGCGAAATTI

CCAACAGGC2l

AAAGAGCAGC

AAAGAAAACC

GGGCAAACCC

*AAAAACTTAC

CTTAATGAA)

ACTGTGGTA(

7GATCAGC"A

CAAACTGTC(

k. CAACCAGGA.

3 CAAGCTGTC(

GGCACGTT

TAGCCCCAGG

CCACGCCAGT

CTAGTGACAA

AccAACcATT rTACAGCAAC

ATTATTACAA

CTGAATTAGT

TAGGGGCCTT

ACCCACTTCC

AAAGAGAACT

GCTATGAATT

AkTTTTGTAGT

ATTATCA.AC

CAGTTTTTGC

TCTTGA-ATCG

ACGCTAAACG

ATCTGTTAGA

CAAT1GCTT'I

AAACAGGTGA

3TAGATGGACP 3CA CCGGGA6AC k. CGCCAAGCGC

TTAAAGCACC

~AGAATGCCTI

3CTCGTTCAT'

PAATATACCT

3 CATTCACTA! 3TTAACGAGA 3CAGCTAGAG,

TCAAACAGGC

AAAATATCGC

CCCATTTGAA

CTTTGAGT

AGCAGGTGCT

GAGTGGCAAA

AGAAATCAAA

AAAAGTAGAA

CGCCAATTTC

AGGAACGATT

A.ACTGCTCAA

AGATGAACTG

GAAGAAGAAT

AGAAGTAATG,

AATTTACAAT

TGCAGGAGAA

GCTAAATGAA

CACAACGCTA

AGGCGATCTT

AGCATTGGCA

LAACCAATCTG

GTATCGT'TI'

AAGCTTTACG

TTT'rATTAAT

SAGCAGGTGCT

C ACGTTCTGAC C TGTGGAALACA r TGCGGCAACA k. ACAACCTGTA k. GACAGGTTGG

S

S

5.5 TCAGCCAACA ACGAAACAAI EF125-4 (SEQ ID NO:468) 5*S*

NFVNYQGTAK

TTNNQGEIVA

KGAFOIVKTN

PKLPDGADYI

KLYRIENGEX

NDKQPLDELE

VSEITTDKTG

NYQGTAQLTK

ETQAPTSYLL

FKVTDAETGQ

KIJKPELVNAG

LII(KDVNGHL

EHLAPGKYRF

SADQPLAGAV

IYPELVKVEI

IFEREVTAEK

FVNYQAEVMG

EIYAKGLNEG

ENETGEALAG

NETPSASFTI

TVARSLRSDN

TFVNEKQPVS

LSGATFKVLD

VETKAPTGYL

FELYDHNKQS

RGDFKGDPEI

DGSLAMEIDLG

R.KVNEQGQTL

HYVLVETKAP

AVPKVIDETG

AKDNQGKPAT

QGLVQVNHLQ

KKTKPNQPTT

AKGETIQTGL

LN'TTPVPFE I

LGITATSGKD

FQLGAFANFI(

AGSYELDELD

AGAVFAIYNA

TGYLLDTTLH

QTVDGQTNLM1

WLKAPFIN'Y

PGKYTFVET(

AEKNAGKPAV

GKI IFRDLA? GRAVFKKI DA

ATDGYIVNKQ

D)EQNQPQGSP

PFDVI'AQLGK

SDKQGKVIAK

QCAAKLVKID

APDGYQL.SKQ

VVASDNFVSY

GTYYYKEIKA

NANPLPGTIF

P IYFVVKKNS

ITFLNRAGEK

EQPIALGDLI

NL.APGTYRFV

QQKNALAGAE

AVAFT IAATA

KQA.ARETG-WLG

EF126-1 (SEQ.ID.NO:469)

TAGCGAAAGA

ATGGTGATTG

GAAGCGGTTA

AGTCTGGCTG

GAAACGGAAC

AAATAGGGAG

TCGCTGGAAC

AAGCAGGAGA

ATTGTAAACG

CAGTCGATTT

GATTAAAATG

AGTTGTGGGA

TACAGAAGGA

GATATTGGAA

AGTAGTI'GTT

TTrAAGAAAG

AATTTCAGTC

ATGACCAATA

GGACAAGCTA

GAAGATGCTA

CAACGAAATT

CCACATTGGC

CGGTGAAAGT

CTTTCCCAGT

GTGGTAGTTT

ATTATCGACA

TTTAGCTGAA

GAPLAGACGAC

TCAAGCGGGT

TTCAGATAAT

234 TABLE 1. N~ucleotide and Amino Acid Seqeuenccs of E. faecalis Genes.

TTTCCACATG

GTGATGCTGG

ATTAATTCAG

AGCCAATTTG

GGATTGA.AAC

ACGTATTTCC

AAGACCAATA

TCAGTGGAAT

GAAkATTACTA

AGTTCAGTGA

GAGTTGCAAC

TTTACAACCC

GCTTCATI'AA

GGAAATGATG

TACGTAGGAA

ACCCITGTAA

ACGATTCCTA

ACAAAAGATA

TGGCATGTCP

GATGACATTP

CATOITACAC

AAACAAGCAC

AAAA'ITAAJ

cccA.AccAAC

ACCGTAACA(

TTAGATTTAX

GAAACAAGCC

ACTGATGTAk

CAAGAAAAT,

AGTGGTCAT,

GAATTAGCA'

AACGAAGGT

GAAGATCCA

CACCAAGAA

CAAGCCAGC

GANGAAAAT

ACTTTAC'I

ATGACTATI

CAACAAGGC

CAT'ICCAAC

GAACCTAAP

ACGAACCCI

ACAACACTI

TTGGCATI

TAAGACAAGC

CTTCATATCG

CTGATTATGA

TCTCTGGTTT

TCGCTTTAGA

TATTAGTGAC

CCAATGATTC

ATAGTAATGA

AC C AGCTA

ATTCATACTT

AAGGGTCTAG

ALATTAAAACA

CGATTGCCAA

TGCCTGTTCA

ACATCACGAT

GTAGTGGGAC

AAAATGACAA

TCGAAAATCA

AAACAGCCTT

ATAAAGTOCT

CTAACGGCAC

ACAGCTATGA

k CTGACGCAAC

;CCGACTTAA-P

:CACCGCCAGI

k CCAACCGCG;

CTTGGACCCI

k AAGTCACAGI k. ACAAAGTAAC AL CGTACACAA' C CTTATATTGQ G ACGTGTTGC A CGATTACAA" T TTAAATGGA.

A TCGTGGATG, C GCAAAGATG' 'A TCAACAAAA 'A CCACTAAAA !G GGATTCCCA A AGCCAACCC iC AACCGCTAA LG TCAATTTTC L.A ATCCACTTI .A CAAAAAAT.P

GATTGATGA.A

CGGCGGAAAA

TATGAATGTG

TGGAGACGTT

TACGTACAAT

AGATGGGGTC

AATCAATGAA

cTACCAAGGT

TGAAATGATT

TGATAAATAT

CACACCAGAA

AATTGTCAAA

TCAATTTGAT

AATTAACGGA

TCACTACGAA

AATGAATCA-A

TGCGCATGCC

AGAACAC'TP

TGGCA.ACGAJ;

AGATATCAT'I

AGTAACACAJ

CTATTTAAGJ

GGACGAAGPI

CTTTGCCAA'

TGATC-CAAA'

TCAAGAAT'FI

AGCCAGCAT(

TGAAAATGG'

TTTTCAAAT,

r' GACCATIAC

ACAAGGTG

k. TTCCAACAA ,k AGATATCGA P. cGTCAAAAC A. CA'ITAATAA T TACAGATAA A AGATGACAG T TAAAACTGA A CCA-AGCCGA T A.ACACCGC A ACCGAAAJ G AAAATCAGC 'A CATCATCGC LA AAAAAGAAJ

GTGTCAAG

CA.ATTITATGT

CGCGTCAATA

CGGACGTATG

cAAACACACG

GCTAATACAC

TATCCAGATC

GCAGCAGCAG

AATCTATT

AAAACAGAAG

GATITATTA

r-ATCGTCTGG

*ATTCAATCTG

*CAAAccA'TTT

*CTCAAAGAAA

GGAACAATTG

TGTGACGTGA

LGATTTA.ACCA

LACCAGTACTT

7GATGTGA.AAG C AAAATAACA

SGGTCATACG'I

k TTAGCGCCT'I C' GAAGGTGACC r' ATTGCTAAAC r AAATG.GAACC .3 GTAGATGACI r AAAGATGTT)

AACAANCAA(

T ACTAAAAMC C ATTCCCAACI GC'ITATCTGA T 'TCCTGATGG A CGCAATTGAC Ir GTGGTACGCC P GAGATTTAAC C GTTTAGATGG 'I CAAGACATCC I AAG'ITTTAGC C GGGAAAGTGT 9

TGGGT-CCTT'

CAAGCCAATC

CGCAATCCACJ

CGACCGCTAC

CAGCALACTAG

ATACACAT

CTAAGGA-AT

CGCCAGAAGA

ATCGTGAAGA

GGACCCAAC

TCACCGACGA

,AAGTAACTT

'ATACAATGAC

ACATTGAACA

TGTTACArTC

ACGTAGAACG

TCAAAACAGC

k TTAATAAAGT k. CAGCTA.ACCG

CNGACAGCTA

k. AAGCTAGCGC

'AAGCCGACTT

:AAGACCGC

k.AGACAAAA

TATGATAAA

A.CCGCCCCA

A.ATCGAAAA

TACTTGCAT

CTTCAAGTC

rTAAACCAA r.AATCTTI'A

GTAAAACAA

ATTGATGAT

CCAGCAACA

;GACGATGCT

ACAGAAGGT

CATGCAGCA

CCAGAACCC

CCAACGA TT

AGTTTCGAT

AGCATGGTC

kAATGGTAAA rGAAATGAAC rATCACCACT

A.GGCGGGATT

:AACAAACCA

ACAAGAACAT

TTTCGGTAAC

GTTAGAcATC

CAAAGTAACA

TGACTATTTA

AACGGACGAA

GAAC'ITTGGC

ACCAACGCCA

AACCAACCGT

CACATGGACC

GAAAGTTNCT

TAAcAAAGTA

TACATACACA

cCCTTATATT

TGACGTGTTG

AAAAAAACCT

TCAAGCACCA

AATGACTAAT

GGCTATTAGC

CCAACCGTAA CACCACCTGC GGCCAAGAAC

ATTTAGATTT

GCTrCGGTA

GTGTTAGACA

GGCATAGTAA

TACTCTTACT

GCAACGGATG

TTAAACTTTG

GCACCAACGC

CCGTTGACC

AGTAAAGGAA

GG'rTTAATTG AA ITAC ACGA;AcAAG

TCACAGACGT

CACAAGAAAA

TAGCTGGTCA

AAGAA'ITAC

GCAACGAAGG

CAGAAGACCC

CTACAAATCA

TTCATTTACC

TCCTTATAGT

EF126-2 (SEQ ID NO:470) MF KXATKLLSTM vIVAGTVVGN~ FSPTLALAEE AVKAGDTEGM TNTVKVKDDS LADCKRILEG QATFPVQAGE TEPVIJVVVE DASGSFSDNF PHVRQAIDEV

VQGLSD)QDRV

MLASYRCGKQ FMFPDGKTKI NSADYDMNVR VNTQLTYDKS QFVSGFGDVR

TYGGTPTAPG

LXLJALDTYNQ THGDLTNRKT YFLLVTDGVA NTRLDGYLHK TNTNDS1INEY PDPRHPLQVS VEYSNDYQGA AAEVLALNQE ITNQGYEMIN AYWESVESLS SVNSYFDKYK TEVGPFVKQE LQQGSSTPED FITSQSIDDF TTQLKQIVKD RLAQSTPATA SLTIANQFDI QSATATDDAG UDVPVQINGQ TISATSTEGY VGNITIHYEV KENTAIrJAAT LVgSGTMNQG TIAXEFPEAT 235 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

I PKNDNAHAC

DINKVLDIID

IKTDATDEEL

DLTNRDQEFK

ENNKVTFEMN

EGDVLHSNKP

ASMVDDINKV

TITTKIKTDA

PKQPLKPK(P

GITKNKKRKN

DVTPEDPTIT

VKVTDENGKD

APYIEQGGIP

WNVKTAFGNE

XQADSYDYLS

TVTPPAPTPE

LDITDVKVXX

TDEELAPYIE

LTPTNHQAPT

KDIENQEHLD

VTANGTVTQE

NQADLNFGNE

TSTWTQASMV

GHTYTMTITT

DPTITKDIEG

ENGKDVTDNG

QGGI PNQADL

NPVNFGKSAS

LTNREDSFDW

NNVTFMK

GDVLHSNKPT

DDINXVLDIT

KIKASATDEE

QEHLDLTNP.D

IVTQENNVT

NFGNEGDVLH

KGIHLPM'TNT

HVKTA.FGNET

QADSYDYLSG

VTPPP'JOPNI

DVKVTIDENCK

LAPYIEQGGI

QEFKWNVKTA

FTMNKXDDSY

SNKPTVTPPA

TVNPLYMIAG

STWTQASMVD

HTYTMTITTK

AKDVEGQEHL

DVTANGKVTQ

PNQADLNFGN

FGNETSTWTQ

SYLAGHTYTM

PTPEDPKKPE

LIVL IVA 1SF EF126-3 (SEQ ID NO:471) 9*

S

59 9.

9 9 @9 59 5S

S.

S S 5 5* 5 0

S

9 .9 9559 5 9* 9 59 @5

TGAA

GAAGCGGTTA

AGTCTGGCTG

CAAACGGAAC

TTTCCACATG

GTGATGCTGG

ATTAATTCAG

AGCCAATT1'G

GGATTGAAAC

ACGTATTC

AAGACCAATA

TCAGTGGAAT

GAAATTACTA

AGTI'CAGTGA

GAGTTGCAAC

TTTACAACCC

GCTTCATTAA

GGAAATGATG

TACGTAGGAA

AAGCAGGAGA

ATTGTAAACG

CAGTCGATTT

TAAGACAAC

CTTCATATCG

CTGATTATGA

TCTCTGC [T TCGC IAGA

TATTAGTGAC

CCAATGATTC

ATAGTAATGA

ACCAAGGCTA

ATTCATACTT

AAGGGTCTAG

AATTAAAACA

CCATTGCCAA

TGCCTGTTCA

ACATCACGAT

TACAGAAGGA

GATATTGGAA

AGTAGTITGTT

GA ETGATGAA

CGGCGGAA.A

TATGAATGTG

TGGAGACCFI'

TACGTACAAT

AGATGGGGTC

AATCAATGAA

CTACCAAGGT

TGAAATGATT

TGATAAATAT

CACACCAGAA

AATTGTCAAA

TCAATTTGAT

AATTAACGGA

TCACTACGAA

ATGACCAATA

GGACAAGCTA

GAAGAT-uCTA

GTGGTTCAAG

CAATTATGT

CGCGTCAATA

CGGACGTATC

CAAACACACG

GCTAATACAC

TATCCAGATC

GCAGCAGCAG

AATGCGTATT

AAAACAGAAC

GATTI'ATTA

GATCGTCTGG

A ETCATCTG

CAAACCATTT

GTCAAAGAAA

CCC TGAAAGT

CTTTCCCAGT

GTGGTAGTTT

CCTTATCTGA

TTCCTGATGG

CGCAA ITGAC

GTCGTACGCCC

GAGATTTAAC

GTTTAGATGG

CAAGACATCC

ALAG=IITAGC

GGGAAAGTGT

TGGGTCCTTI

CAAGCCAATC

CGCA.ATCGAC

CGACCGCTAC

CAGCAACTAG

ATACAGCGAT

GAAAGACGAC

TCAAGCGGGT

FI'CAGATAAT

TCAAGACCC

AAAGACAAAA

TTATGATAAA

AACCGCCCCA

GAATCGAAAA

TI'ACTTGCAT

TCTTCAAGTC

GTTAAACCAA

TGAATCTTA

TCTAA.AACAA

TATTGATGAT

ACCAGCAACA

GGACGATGCT

TACAGAAGGT

TGAT

EF126-4 (SEQ ID NO:472) EE AVKACDTEGM TNTVKVKDDS LADCKRILEC QATFPVQAGE TEPVDLVVVE MLASYRGGKQ FMFPDGKTKI NSADYDMNVR LKLALDTYNQ THGDLTNRKT YFLLVTDGVA VEYSNDYQGA AAEVLALNQE ITNQGYEMIN LQQGSSTPED FITSQSIDDF TTQLKQIVKD NDVPVQINGQ TISATSTEGY VGNITIHYEV

DASGSFSDNF

VNTQLTYDKS

NTRLDGYLHK

AYWTESVESLS

RLAQSTPATA

I{ENTAID

PHVRQAIDEV

QFVSGFGDVR

TNTNDS INEY

SVNSYFDKYX

SLTIANQFDI

VQGLSDQDRV

TYGGTPTAPG

PDPRHPLQVS

TEVGPFVKQE

QSATATDDAG

EF127-1 (SEQ ID NO:473)

TAGCGAAAGA

ATGGTGATG

CAAGCGGITA

AGTCTGGCTG

GAAACGGAAC

T'ITCCACATG

GTGATCCTGG

AAATAGGGAG

TCGCT'GGAAC

AAGCAGGAGA

ATTGTAAACG

CAGTCGATTT

TAAGA'.AAGC

C?1TCATATCG

GATTAAAATG

AGTTGTGGGA

TACAGAAGGA

GATATr'GGAA

AGTAGI'GTT

GATGATGAA

CGCGAAAA

TTTAAGAAAG

AATITMAGTC

ATGACCAATA

CGACAAGCTA

GAAGATr.CTA GTGGTrCAAG

CAAMATGT

CGCGTCAATA

CAACGAAATT

CCACATTGC

CGGTGAAAGT

CTTTCCCAGT

GTGGTAGTTT

GCTTATCTGA

TrCCTGATGG

CGCAATTGAC

ATTATCGACA

'TTTAGCTCA

GAAAGACGAC

TCAAGCGGGT

TrcAGATAAT

TCA.AGACCGC

AAAGACAAAA

TTATGATAAA

ATTAATTCAG CTGATTATGA TATGAATGTC 236 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

2 kGCCAATTTG TCTCTGG'TT I 3GATTGAAAC TCGCPTTAGA k.CGTATTITCC TATTAGTGAC A~ %.AGACCAATA CCAATCA'PTC rcAGTGGAAT ATAGTAATGA C 3AAATTACTA ACCAAGGCTA TI !LGT-TCAGTCA A'ITCATACTT I 3 AGTTGCAAC AAGGGTCTAG C TTTACAACCC AATTAAAACA GCTTCATTAA CGATGCCCAA IJ GGAAATGATG TGCCTGTTCA2 TACGTAGGAA ACATCACGAT ACCCTTGTAA GTAGTG3GGAC ACGATTCCTA AAAATCACAA ACAAAAGATA TCGAAAATCA TGGCATGTCA AAACAGCCTI' CATGACATTA ATAAAGTGCT GATG'FrACAG CTAACGGCAC AAACAAGCAG ACAGCTATGA AAAATI'AAAA CTGACGCAAC CCCAACCAAG CCGACTTAA ACCGTAACAC CACCGCCAGT TAGATTTAA CCAACCGCGA GAAACAAGCA CTI'GGACCCA ACTGATGTAA AAGTCACAGA CAAGAAAATA ACAAAGTAAC AGTGGTCATA CGTACACAAT GAATTAGCAC CTI'ATATTGA AACGAAGGTG ACGTGTTGCA GAAGATCCAA CGA'ITACAAA GACCAAGAAT TTAAATGCAA CAAGCCACCA TGGTGGATGA GANCAAAATG GCAAAGATG-T ACTTTTACTA TGAACAAAAA ATGACTA?1TA CCACTAAAAT CAACAAGGCG GGATTCCCAA CATTCCAACA AGCCAACCGT GAACCTAAAC AACCGCTAAA ACGAACCCAG TCAATTTTGG ACAACAGTAA ATCCACTTTA T=rGGCATAA CAAAAAATAA

YGGAGACGTT

'ACGTACAAT

4

GATGGGGTC

LATCAATGAA

TACCAAGGT

~GAAATGATT

~GATAAATAT

:ACACCAGAA

=.TTTCAAA

~CAATTTGAT

ATTAACGGA

rCACTACGAA kATGAATCAA

EGCGCATGCG

AGAACACTTA

rGGCAACGAA

AGATATCATT

PGTAACACAA

CTATTTAAGT

GGACGAAGAA

CTTTGGCAAT

TGATCCAAAT

TCAAGAATI'

AGCCAGCATG

TGA.AAATGGT

TITTGAAATG

GACCATI'AC7

ACAAGGTGGC

rCCAACAAA

AGATATCGAP

CGTCAAAACP

CATITAA.TAA;

TACAGATAAI

AGATGACAGC

TAAAACTGAC

CCAAGCCGAC

AACACCGCC'

ACCGAAAAA2

AAAATCAGCJ

CATGATCGCJ

AAAAAGAAA)

CGGACGTATG

CP.AACACACG

GCTAATACAC

TATCCAGATC

GCAGCAGCAG

AATGCGTATT

AAAACAGAAG'

GA LT=A'TA

GATCGTCTGG

ATTCAATCTG

CAAACCATT

GTCAAAGAAA

GGAACA

TGTCACGTGA

GA=TAACCA

ACCAGTACTT

CATr.TGAAAG

GAAAATAACA

GGTCATACGT

rrAGCGCCTT

GAAGCTGACC

ATTGCTAAAG

AA.ATGGAACG

GTACATGACA

AAAGATGTrA

AACAANCAAG

ACTAAAATCA

ATTCCCAACt

*CCAACCGTA-A

GGCCAACAAC

LGCTTTCGGTP

GTGTTAGACP

GGcATATAP

TACTCTTACI

GCAACGGATC

TTAAACTM

r' GCACCAACGC k CCGTTGACG( kAGTAAAGGA2 GGTrrAA=r k. AATI'AG

GTGTACGCC

GAGATTTAAC C GTTTAGATGG I CAAGACATCC IJ

AAGTTTTAGCC

GGGAAAGTGT

-GGGTCCTT

CAAGCCAATC

CGCAATCGAC

CGACCGCTAC

CAGCAACTAG

ATACAGCGAT

CTAAGGAAT

CGCCA6AAGA

ATCGTGAAGA

GGACCCAAGC

TCACCGACGA

AAGTAACT

ATACAATGAC

ACATTGAACA

TGTTACAI1TC

ACGTAGAAGG

TCAAAACAC

TTAATAAAGT

CAGCTAACGG

CNGACAGCTA

AAGCTAGCGC

AAGCCGACTT

CACCACCTGC

ATTTAGAT

ACGAAACAAG

TCACAGACGT

CACAAGAAAA

TAGCTCGTCA

AAGAATTAGC

GCAACGAAGG

CAGAAGACCC

CTACAAATCA

k. TTCATTTACC

'TCCTATAGT

LACCGCCCCA

;AATCGAAAA

~TACTTGCAT

LCFI'CAAGTC

;TTAAACCAA

EGAATCTTA

rGTAAAACAA rATTGATC-AT kCCAGCAACA jGACGATGCT

TACAGAAGGT

TGATGCAGCA

TCCAGAAGCG

TCCAACGATT

TAGTTTCGAT

CAGCATGGTG

A.AATGGTAAA

TGAAATGAAC

TATCACCACT

AGGCGGGATT

CAACAAACCA

ACAAGAACAT

TTTCGGTAAC

GTTAGACATC

CAAAGTAACA

TGACTATTTA

AACCGACGAA

GAACTTTG'GC

ACCAACGCCA

AACCAACCGT

CACATGGACC

GAAAGTI'NCT

TAACAAAGTA

TACATACACA

GCCTTATATT

TGACGTGTTG

AAAAAAACCT

TCAAGCACCA

AATGACTAAT

GGCTA'ITAGC

0* 9O S

S@

0e S S

SO

0@ 5 S

SS

@0 4~ S

S

@5 S S

S

0 *5@S 5

SOS.

OS

5555

S

@505

S

5.55 0 @555 55 0

S.

SO

EF127-2 (SEQ ;D NO:474) MF KKATKLLSTM VIVAGTVVGN FSPTLALAEE AVKAGDTEGM TNTVKVKDDS

LADCKRILEC

MLASYRGGKQ

LKLALDTYNQ

VEYSNDYQGA

LQQGSSTPED

NDVPVQ INGQ

IPKNDNAHAC

DINKVLDI ID

IKTDATDEEL

QATFPVQAGE

FMFPDGKTKI

THGDLTN~RKT

AAEVLALNQE

FITSQSIDDF

TISATSTEGY

DVTPEDPTIT

VKVTDENGKD

APYIEQGGIP

TEPVDLVVVE

NSADYDDIVR

YFLLVTDGVA

ITNQGYEMIN

TTQLKQIVKD

VGNITIHYEV

KDIENQEHLD

VTAN'GTVTQE

NQADLNFGNE

DASCSFSDNF

VNTQLTYDKS

NTRLDGYLHK

AYWESVESLS

RLAQSTPATA

KEN'rAIDAAT

LTNREDSFDW

NNKVTFEMNK

PHVRQAIDEV

QFVSGFGDVR

TNTNDS INEY

SVNSYFDKYK

SLTIANQFDI

LVSSGTMNQG

HVKTAFGNET

QADSYDYLSG

VQGLSDQDRV

TYGGTPTAPG

PDPRHPLQVS

TEVGPFVKQE

QSATATDDAG

TIAKEFPEAT

STWTQASMVD

HTYTMTITTK

GDVLHSNKPT VTPPPVDPNI AXDVEGQEHL 237 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

DLTh?.DQEFK

ENNKVTFEN

EGDVUISNKP

ASMVDDINKV

TITTKIKTDA

PKQPLKPKKP

G ITKNKKRKN

WNVKTAFGNE

XQADSYDYLS

TVTPPAPTPE

LDITDVKVXX

TDEELAPYI E

LTPTNHQAPT

TSTWTQASMV

GHTYTMT ITT

DPTITKDIEG

ENGKDVTDNG

QGG:?NQACL

NPVNIPGKSAS

DDINKVLDIT

K IKASATDEE

QEHLDLTNRD

IVTQENN'KVT

NFGNEGDVLH

KGIHLPMT-NT

DVKVTDENGK

LAPYIEQGGI

QEFK7WVKTA

FTMKKDDSY

SNKPTVTPPA

TVNPLYM TAG

DVTANGKVTQ

PNQADLNFGN

FGNETSTWTQ

SYLAGHTYTM

PTPEDPKKPE

LIVLIVAISF

EF127-3 (SEQ ID NO:475) GAATCAA GGAACA.ATTG CTAAGG;AATT TCCAGAAGCG

ACGATTCCTA

ACAAAAGATA

TOGCATGTCA

GATGACATTA

GATGTTACAG

AAACAAGCAG

A-A-ATTAAAA

CCCAACCAAG

ACCGTAACAC

TTrAGATTTA6A

GAAACAAGCA

AAAATGACAA

TCGAAAATCA

AAACAGCCTT

ATAAAGTGCT

CTAACGGCAC

ACAGCTATGA

CTIGACGCAAC

CCGACTTAAA

CACCGCCAGT

CCAACCGCGA

CTrGGACCCA

TGCGCATGCG

AGAACACTTA

TGGCAACGAA

AGATATCATT

AGTAACACAA

CTATTTIAAGT

GGACGAAGAA

CTTTGGCAAT

TGATCCAAAT

TCAAGkAAI'

AGCCAGCATG

TGTGACGTGA

GATTTAACCA

ACCAGTAC'T

GATIGTGAAAG

GAAAATAACA

GGTCATACGT

TTAGCGCCTT

GAAGGTGACG

ATTGCTAAAG

AAATGGAACG

GTAGATGACA

CGCCAGAAGA

ATCGTGtAAGA GGACCCA6AGC

TCACCGACGA

AAGTAACTTT

ATACAATGAC

ACATTGAACA

TGTTACATTC

ACGTAGAAGG

TCAAAACAGC

TTAAT

TCCAACGATT

TAGTTTCGAT

CAGCATGGTG

AAATGGTAAA

TGAAATGAAC

TATCACCACT

AGGCGGGATT

C AAC AAAC CA

ACA.AGAACAT

TTTCGGTAAC

EF127-4 (SEQ ID NO:476) MQG TIAKEFPEAT IPKNDNAHAC

DVTPEDP'TIT

DINKVLDI ID VKVTDENGKD IKTDATDEEL APYIEQGGI P DLTNRDQEFK WNVKTAFGNE

KOIENQEHLD

VTANGTVTQE

NQADLNFGNE

TSTTQASMV

LTNREDSFDW

NNKVTFEMNK

GDVLHSNKPT

DDIN

HVKTAFGNET STWTQASMVD QADSYDYLSG HTYTMTITTK VTPPPVDPNI

AKDVEGQEHL

EF128-1 (SEQ ID NO:477) TAGCGAAAGA AAATAGGGAG GATTAAAATG ATGGTGATI'G TCGCTGGAAC AGTTGTGGGA GAAGCGGTTA AAGCACGAGA TACAGAAGA AGTCTGGCTG ATTGTAAACG GATATTGGAA GAAACGGAAC CAGTCGATTT

AGTAGTT.

TTTCCACATG TAAGACAAGC GAT=KTGAA GTGATGCTGG CTTCATATCG CGGCGGAAAA ATTA.ATTCAG CTGATTATGA TATGAATGTG AGCCAATTTG TCTCTGGTTT TGGAGACGTT GGATTGAAAC TCGCTTTAGA

TACGTACAAT

ACGTATTTCC TATTAGTGAC AGATGGGGTC AAGACCA.ATA CCAATGATTC AATCAATGAA TCAGTGGAAT ATAGTAATGA CTACCAACGT GAAATTACTA ACCAAGGCTA TGAAATGATE AGTTCAGTGA ATTCATAC~TI TGATAAATA'I GAGTTrGCAAC AACGGTCTAG CACACCAGA.P TTI'ACAACCC AAITTAAAACA AATTGTCAAP GCTTCATTAA CGATTGCCAA

TCAATTTGAI

GGAAATrGATG TGCCTGT1'CA

AATTAACCGG;

TTTAAGAAAG

AATL'TCAGTC

ATGACCAATA

GGACAAGCTA

GAAGATGCTA

GTGG'ITCAAG

CAATI'ATGT

CGCGTCAATA

CGGACGTATG

CAAACACACG

GCTAATACAC

TATCCAGATC

CAGCAGCAG

AATGCGTA'Fr

*AAAACAGAAG

GA T1'=ATTA

LGATCGTCTGG

ATrCAATCTG

CAAACCATTI

CAACGAAATT

CCACATI'GGC

CGGTGAAAGT

CTTTCCCAGT

GTGGTAGTTT

GCT'TATCTGA

TTCCATGG

CGCA.ATTGAC

GTGGTACGCC

GAGATTTAAC

G=TAGATGG

CAAGACATCC

AAGTITTAGC

GGGAAAGT

TGGGTCCT'T

CAAGCCAATC

CGCAATCGAC

CGACCGCTAC

CAGCAACTAG

ATTATCGACA

TTTAGCTGAA

GAAAGACGAC

TCAAGCGGGT

TTCAGATAAT

TCAAGACCGC

AA.AGACAAAA

ETATGATAAA

AACCGCCCCA

GAATCGAAAA

TTACTTGCAT

TcTTCAAGTC

GTTAAACCAA

TCAATcTTTA

TTAAAACAA

TAT GATGAT

ACCACCAACA

GGACGATGCT

TACAGAAGGT

238 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faeculis Genes.

TACGTAGGAA

ACCCTTGTAA

ACGATTCCTA

ACAAAAGATA

TGGCATGTCA

GATGACAI1TA

GATGTTACAG

AAACAAGCAG

AA.AATTAAAA

CCCAACCAAG

ACCGTAACAC

T1'AGAT1'AA

GAAACAAGCA

ACTGATGTAA

CAAGAAAATA

AGT-GG-TCATA

GAATTAGCAC

AACGAAGGTC

GAAGATCCAP

GACCA.AGAAI

CAAGCCAGC;

GANGAAAATC

ACTTTI'ACTI

ATGACTAT'IU

GAACAAGGC(

CATTCCAACi

GAACCTAAAI

ACGA.ACCCAI

ACAACAGTA

TTTGG-CATA

ACATCACGAT

GTACTGGGAC

AAAATGACAA

TCGAAAATCA

AAAcAGcCTT

ATAAAGTGCT

CTAACGGCAC

ACAGCTATGA

CTGACGCAAC

CCGACTTAAA

CACCGCCAGT

cCAACCGCGA

CTTGGACCCA

AAGTCACAGA

ACAAACTAAC

CGTACACAAT

CTTATATTGA

ACGTGTI'GCP

CGATTACAAJ

TTA.AATGGA-Z

LTGGTGGATG;

GCAA-AGATM'

STGAACAAAAJ

~CCACTAAAA'

3 GGAITCCCAj k. AGCCAACCG'

AACCGCTAA

2TCAAITI'TGI NATCCAC TT.

A CAAAAAATA

TCACTACGAA

AATGAATCAA

TGCGCATGCG

AGAACACTTA

TGGCAACGAA

AGATATCATT

AGTAACACA-A

CTA'FIAAGT

GGACGAAGAA

CTTTGGCAAT

TGATCCAAAT

TCAACAATTT

AGCCAGCATG

TGAAAATGGI

TTTTGAAATC

GACCATTACI

ACAAGGTGGC

TTCCAACAAI

LAGATATCGAJ

CGTCAA.AACI

CATTAATA.AJ

STACAGATAAX

k AGATGACAG r TAAAACTGAI k CCAAGCCGA' r AACACCGCC' k. ACCGAAAAA 2AAAATCAGC A CATGATCC A AAAAAGAAA GTCAAkAGAAA

GGAACAA'FTG

TGTGACGTGA

GATrI'AACCA ACcAGTACTT

GATGTGAAAG

GAAAATAACA

GGTCATACGT

TTACCGCCTT

GAAGGTGACG

ATTGcTAAAG

KAATCA.ACG

GTAGATGACA

AAAGATGTTA

AACAANCAAG

ACTAAAATCA

ATTCCCAACC

CCAACCGTA-A

kGGCCAAGAAC k. GCTTTCGGTP

~GTGT'TAGAC;

r GGCATAGTA;

TACTCTTACI

GCAACGGAIX

TTAAACTTT

T GCACCAACG( A CCGTTGACGI A AGTAAAGGA A GGTTAATT A AATTAG ATACAGCGAT T CTAkAGGAAT

T

CGCCAGAAGA

TI

ATCCGTGAAGA GGACCCAAGC C TCACCGACGA AAGTAACTTT I ATACAATGAC 9J

ACATTGAACA

TGTTACATTIC

ACGTAGAAGG

TCA-AAACAGC

TTAATAAAGT

cAGCTAACGG

CNGACAGCTA

AAGCTAGCGC

AAGCCGACTT

CACCACCTGC

ATTTAGAT

ACGA.AACAAG

TCACAGACGT

CACAAGAAAA

TAGCTGGTCA

AA!GAATTAGC

SGCAACGAAGG

cAGAAGACCc 2CTACAAATCA N, TTCATTTACC G TCCTTATAGT

GATGCAGCA

CCAGAAGCG

'ccAACG-ATT

'AGTTTCGAT

AGCATGGTG

LAATGGTAAA

~GAAATGA.AC

~ATCACCACT

kGGCGGGATT

AACAAACCA

kCAAGAACAT

TTCGGTAAC

GTTAGACATC

:AAAGTA-ACA

TGACTAITI'A

AACGGACG-AA

GAACTTT GGC

ACCAACGCCA

AACCA-ACCGr

CACATGGACC

GAAAGTI'NCT

TAACAAAGTA

TACATACACA

GCCTTATATT

TGACGTGTTG

AAAAAAACCT

TCAAGCACCA

AATGACTAAT

GGCTATTAGC

EF128-2 (SEQ ID NO:478) Mr vvhqvPCT'M UT\JAGTVVCN FSPTLALAEE AVKAGDTEGM

TNTVKVKDI

LADCKRILEG

MLASYRGGKQ

LKLALDTYNQ

VEYSNDYQGA

LQQGSSTPED

NDVPVQINGQ

IPKNDNA}HAC

TINKVLDIID

IKTDATDEEL

OLTNPDQEFK

ENNKVTFEMN

EGDVLHSNKP

ASMVDDINKV

TITITKIKTDA

PKQPLKPKKP

GITKNKKRKN

QATFPVQAGE

FMFPDGKTKI

TI4GDLTNRKT

AAEVL.ALNQE

FITSQSIDDF

TISATSTEGY

DVTP-7DPTIT

VKVTDENGKD

APYIEQGGIP

WN'JKTAFGNE

XQADSYDYLS

TVTP PAPTPE

LDITDVYKVXX

TDEELAPYI E

LTPTNHQAPT

TEPVDLVVWE

NSADYDMNVR

YFLLVTDGVA

ITNQGYEMIN

TTQLKQIVKD

VGNITIHYEV

KDIENQEHLO

vTANGTVTQE

NQADLNFGNE

TSTWTQASMV

GHTYTMTITT

DPTITKDIEG

ENGKDVTDNG

QGGIPNQADL

NPVNFGKSAS

DASGSFSDNF

V~NTQLTYDKS

NTRLDGYJHK

AYWESVESLS

RLAQSTPATA

KENTAIDAAT

LTNREDSFDW

NNKVTFEMNK

GDVT-4-SNKPT

DDINKVLDIT

KIKASATDEE

QEHL.DLTNRD

IVTQENNKVT

NFGNEGDVLH

KGIHLPMTNT

QFVSGFGDVR

TNTNDS INEY svNsYFDXK

SLTIANQFDI

LVSsGTMNQG

HVKTAFGNET

QADSYDYLSG

VTPPPVDPNI

DVKVTDENGK

LAPYIEQGGI

QEFKWNVKTA

FTMM<KDDSY

SNKPTVTPPA

TVNPLYMIAG

VQGLSDQDRV

TYGGTPTAPG

PDPRHPLQVS

TEVGPFVKQE

QSATATDDAG

TIAKEFPEAT

STWTQASMVD

HTYTMTITTK

AKDVEGQEHL

DVTANGKVTQ

PNQADLNFGN

FGNETSTWjTQ

SYLJAGHTYTM

PTPEDPKKPE

LIVLIVAISF

EF128-3 (SEQ ID NO:479) 239 TABLE 1. Nucleotide arnd Arnino Acid Seqeuences of E. faecalis Genes.

AGA TGAAAATGGT AAAGATGTI'A CAGCTAACGG

CAAAGTAACA

CAAGAAAATA ACAAAGTAAC TITT-GA-AATG AACAANCAAG

CNGACAGCTA

AGTGGTCATA CGTACACAAT GACCATTACT ACTAAAATCA

AAGCTAGCGC

GAATTAGCAC C1TTATATTGA ACAAGGTGGC ATTCCCAACC

AAGCCGACTT

AACGAAGGTG ACGTG-TGCA TTCCAACAAA CCAACCGTA.A

CACCACCTGC

GAAGATrcCAA cGATTACAAA AGATATCGAA GCCAAGA-AC

ATTAGATTT'

GACCAAGAAT TI'AAATGGAA CGTCAAAACA GCTTTCGGTA

ACGAAACAAG

CAAGCCAGCA TGGTGCATGA CMTTAATAAA GTGTTAGACA

TCACAGACGT

GANGAAAATG GCAAAGATGT TACAGATAAT GGCATAGTAA

CACAAGAAAA

ACTTTTACTA TGA.ACAAAAA AGATGACAGC TACTCTTACT

TAGCT.GGTCA

ATGACTAT'rA CCACTAAAAT TAAAACTGAC GCAACGGATG

AAGAATTAGC

GAACAAGGCG GGATTCCCAA CCAAG.CCGAC -iTAAAcTTTG

GCAACGAAGG

CAITCCAACA AGCCAACCGT AACACCGCCT GCACCAACGC

CAGAAGACCC

GAACCTAAAC AACCGCTAAA ACCGAAAAAA CCGTI'GACGC

CTACAAATCA

ACGA.ACCCAG TCAA'TrrGG AAAATCAGCA AGTAAAGGAA

TTCAT

TGACTATTTA

AACGGACCAA

GAACTTTGGC

ACCAACGCCA

AACCAACCGT

CACATGGACC

GAAAGTTNCT

TAACAAACTA

TACATACACA

GCCTTATATT

TGACGTGTTG

AAAAAAACCT

TCAAGCACCA

EF128-4 (SEQ ID NO:480) DENGK DVTANGKVTQ ENMKVTFEMN

XQADSYDYLS

EGDVLHSNKP

TVTPPAPTPE

ASMVDDINKV

LDITDVKVXX

TITTKIKTDA

TDEELAPYIE

PKQPLKP~kP

LTPTNHQAPT

GHTYTMT ITT

DPTITKDIEG

ENGKEDvTDNG QGG IPNQADL

NPVNFGKSAS

KIKASATDEE

QEHLDLTNRD

I'JTQENNKVT

NFGNEGDVLH

KGI H LAPY IEQGGI

QEFKWNVKTA

FTMNKKDDSY

sNI(PTvTPPA

PNQADLNFGN

FGNETSTWTQ

SyLAGHTYTM

PTPEDPKKPE

*0S EF129-1 (SEQ ID NQ:481)

TGACAAGTGA

ATTGCTACCC

CAAGAAATT

GAAGCAGAAG

CTAAAAGCAA

AAACGTAACC

ACAATGCTAG

GCTGTTTCAA

CAAGCCCTTG

GAAGCTGAAT

ATGAAAGCTT

AAACAAAAAG

GCTGAAAAAG

CCAGTTGCCIl

GAATCAAGCP

GAAAATACTIC

GGAAATAATC

GCGCCTTCTC

CGTCCAGTAC

GAAGCAGGT(

TGGGCGCCA(

TCAGGAAGCC

TACTCAGGT

AGAAACGTCT

cAAGCATCGC

CATCAFI'AAA

TATC'ITCAGT

AATCAGAACA

AAGCAATCAA

ATGCAGTTTTi~i

CAATCGTAAG

TTGATAAAAA

TAGAAACAAA

CATTAGCATT

CAGCTGCTGA

CCAA.ACAAGC

CTTCATCAAC

CGCAACAAAC

GCTCTTCTTC

GTGGCCAAAC

CTGATCCAAC

TATGGGATIGC

;GCATTCCAAA

3GTAACTCAG'I

GTCACCGTGP

k. GCACAATCGI

ATGCATCA

TITGGCGGAC

AGCAAAACAA.

ATTTGATGAA

ATTACAACAA

AAATCAAGCA

AGATGCGGAC

TGCCAACAAC

AGCTGAAAAC

ACGTCAAGAT

AGAACAATCA

AGCAGAGCAA

TGCTGCAAAA

AACAGAAGCA

AACTGAAACA

ATCAGAACAA

TGGTGGTGGP

AATCAATGC;

AGGTTGC;

*TGATCACTGC

*AATCATGGCC

LTTGGGAAAT'

'AGGACACTC

GTATTACTAT

AATGTTGATA

GGGGATTTAG

AGCATGGCTT

GAAATI'ACAA

CGTGATGTTC

TCAGTTGCAG

GACTTAATGC

GAGAAAAAAG

TTACTITCTA

TCAGCTGAAA

GCACGCTTAG

CCAGCTAAAG

*CAAGCACCAG

*ACTACACCAA

CCAGTACAAC

ACAGTTACAC

TTGAACGTTC

GCTTCTGCA?

TCTCGTGGA(

STGGTACAATC

C AACCCAGGTj k GCCTAA GTTCATTAAC C

AAAAAATTGA

C ETCACAAGT

TACGTGAACA

ACTI'GAATCA

AAGT'rAATGG

ATGCAATCAG

AACAACAAAA

TGAAACAACT

A-ACAATCTGA

GTTCTAAAGC

CTGCTGAACA

CTGAAGTGAA

CAAGCTCAAG

GTACAGATAA

CTACAACACC

CAACACCAGA

TACGTCAATC

CTGCTCGTGC

ATGAAGTTAT

AAACAAACAT

T 'TACGCGTGT

;CTATCAGCA

kGAAAAAAAT k.TCTTcTTTA kAAGCAAACA

ACGTATTGAA

ACAAAGCACA

:cGTGTTCAA

AGAAGACAAA

TGAAGCAACA

ATTAAACGTA

TGGCTTAGAA

AAAAGCTGCA

AGCAGAAGCA

CTCAGCAACT

TAGTGcAACA

AAGCGATAAT

ACCAAcACCA

ATTAGGT'ITA

ACCACAAGTT

CGCAATTATG-

GGTAACAGCT

CGGTTGGT

EF129-2 (SEQ ID NO:482) 240 TABLE 1. Nucleotidc and Amino Acid Seqeuences of E. faecalis Genes.

VYX-KPLFASV LLCSLTLSAI ATPSIALAEN VDKKIEEKNQ EISSLKAXQG DLASQVSSLE

AEVSSVFDES

MLDAVLDADS

AELETKRQDL

EKAKQAAAKP

NTGSSSSEQP

PVVWDAGLAA

GSGHROWEIN

MALREQKQTL

VADAISRVQA

LSKQSELNVM

AKAEVXAEAP

VQPTTPSDNG

SATARAAQVE

PG ITRVGFGY

KAKSEQLQQE

VSTIVSANND

KASLALEQSS

VASSSI TEAQ

NNGGQTGGGT

AGGIPNDHWS

SGSTIVGHSA

ITNLNQRIEK

LMQQQKEDKQ

AESSYXAGLEK

APASSS SATE

VTPTPEPTPA

RGDEVIAIMW

AWVDKKAENE

QKAAAEAEQA

SSTQQTTETI'

P SAD PT INAL

APGNSVIMAW

KKVKQLEATE

RLAAEQKAAA

TPSTONSATE

NVLRQSLGLR

YNETNMVTAS

EF129-3 (SEQ ID NO:483) GGAC AATGTTGATA AAAAAATTGA AGAAAAAAT--

CAAGAAATTT

GAACCAGAAG

CTAAAAGCAA

AAACGTAACG

ACAATGCTAG

GCTGTTTCAA

CAAGCCGTTG

CAAGCTGAAT

ATGAAAGCTI'

AAACAAAAAG

GCTCAAAA.AG

CCAGTTGCCT

GAATCAAGCA

GAAAATACTG

GGAAATAATC

GCGCCTI'CTG

CGTCCAGTAG

CAAGCAGGTG

TGGGCGCCAG

TCAGGAAGCG

CATCATTAAA

TATCTTCAGT

AATCAGAACA

AAGCAATCAA

ATGCAGTI=

CAATCGTAAG

TTGATAAAAA

TAGAAACAAA

CATTAGCATT

CAGCTGCTGA

CCAAACAAGC

CTTCATCAAC

CGCAACAAAC

GCTCTTCTTC

GTGGCCAAAC

CTGATCCAAC

TATGGGATGC

GCATTCCAAA

GTAACTCAGT

GTCACCGTGA

a

C

a a a a.

AGCAAAACAA

ATTT'GATGAA

ATTACAACAA

AAATCAAGCA

AGATGCGGAC

TGCCAACAAC

AGCT'AAAAC

ACGTCAAGAT

AGAACAATCA

AGCAGAGCAA

TGCTGCAAAA

AACAGAAGCA

AACTGAAACA

ATCAGAACAA

TGGTGGTGGA

AATcAATGCA

AGG=TGGCA

TGATCACTGG

AATCATGGCC

TTGGGAAATIl

AGGACACTC.P

GGGGATTI'AG

AGCATGGCTT

GAAATTACAA

CGTGATG~rC

TCAGTTGCAG

GACI-rAAT'GC

GAGAAA.AAAG

TTACTTTCTA

TCAGCTGAAA

GCACGCTTAG

CCAGCTAAAG

CAAGCACCAG

ACTACACCAA

CCAGTACAAC

ACAGTTACAC

'rAACG~rC GCTrCTGCAA

TCTCGTGGAG

TrGGTACAATG

AACCCAGGTA

GCC

CTTCACAAGT

TACGTGAACA

ACTTGAATCA

AAGTTAATGG

ATGCAATCAG

AACALACAAAA

TGAAACAACT

AACA.ATCTGA

GTTCTAAAGC

CTGCTGAACA

CTGAAGTGA-A

CAAGCTCAAG

GTACAGATAA

CTACAACACC

CAACACCAGA

TACGTCAATC

CTGCTCGTGC

ATGAAGTTAT

AA.ACAAACAT

TTACGCGTGT

ATCTTCTTTA

AAAGCAAACA

ACGTATTGAA

ACAAAGCACA

CCGTG'ITCAA

AGAAGACAAA

TGAAGCA6ACA

ATTAAACGTA

TGGC'ITAGAA

AAA.AGCTGCA

AGCAGAAGCA

CTCAGCAACT

TAGTGCAACA

AAGCGATAAT

ACCAACACCA

ATTAGGTTTA

AGCACAAGT'

CGcAAITATG

GGTAACAGCT

CGGTT1TT1GGT TACTCAGGTA

GCACAATCGT

EF129-4 (SEQ ID NO:484) DN VDKKIEEKNQ EISSLKAKQG DLASQVSSLE

AEVSSVFDES

MLDAVLDADS

AELETKRQDL

EYXkKQAAAKP

NTGSSSSEQP

PVVWDAGLAA

GSGHRDWEIN

MALREQKQTL

VADAISRVQA

LSKQSELNVM

AKAEVKAEAP

'JQPTTPSDNG

SATARAAQVE-

PGITRVGFGY

KAXSEQLQQE

VSTIVSANND

KASLALEQSS

VASSSTTEAQ

NNGGQTGGGT

AGGIPNDHWS

SGSTIVGHSA

ITNLNQRIEK

LMQQQKEDKQ

A.ESSKAGLEJ(

APASSSSATE

V'TPTPEPTA

RGDEVIAIMW

RNE-AIXNQAR

AVVDKAENE

QKAAAEAEQA

SSTQQTTETT

PSADPTINAL

APGNSVIMAW

DVQVNGQSTT

KKVKQLEATE

RLAAEQKAAA

TPSTDNSATE

NVLRQSLGLR

YNETNMVTAS

EF130-1 (SEQ ID NO:485)

TGATACATTA

TI'AATCAACA

CAGATACGTA

CCAGCTGATG

TCCTTGAGCA

GGTGCCATTG

GTCGCTTTAT

AAAGGAGGGA

GTTTATTAGT

GTTGGGTGGT

TGAAGAAAAA

CAGAAGCGGT

CGATACCMAG

TAACTGGTGC

AAATATGCGC

TTTACTATTT

TCAACAAAAT

TATGGCTCGT

GATGAAACCC

TGTCGAAATT

CGGGACCATG

CCAAAAGAGA

ATCATTGGCT

ACCCCCTCGT

GAAACAACGT

CAT'rAAA

AATI'TGCCCA

AAAGAAGATC

AAAAAAGAGG

TAGCcTTAAT

ACGCCGTTAG

TTGAC TI'GA

ACAAAAACT

TTTTTAAAGG

AAGTCATGCG

AAAAAATTGG

TTTTAAcAAT

CAAGTTGAAA

TTcAGTTGAG

ACCTGTGATIT

ATTGTCCAAT

GAAAAACAAT

241 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

TATGCCITrGG

ACCAAAAAAG

ACCT'GTAG

AATATGATTA

GGAACATTIAG

CAATTGGAGC

CTAGTCATCG

ACGAACTCAT

AAAAAATCGA

CCTTAAMTAC

CAGCAACGAC

AAAAAACTT

AACGGA-AGAT

TTATATCACT

ACCAACCCCT

CTGTGGCGAT

GCCTATLAAA

AGCCGATTGG

GGCGTT'TTCCT

GATTTATCTA

G'ITGAGTTAA

TTACAAGCAA

GACGCCAACG

GTGGCTTAA

TATTTCACC

CTGTTTATAC

TGATGACGT

CGACGCGAAT

ACGATATGTT

TTTAGAAAGA

ATACAAAATA

TCCTGGTCAA

TGCTGTTCAA

GAAGGC=TC

EF130-2 (SEQ ID NO:486)

YIKRRENMRP

ADVKKNMARE

ALLTGAGTMK

SVEKIEPTRV

LEQXTLADWV

KE-KKRGKNWL

TTFDFDSVES

EDQVMGKNNY

ELI DDVPGQN INSL.LVLLFI IGLALIFNNQ LSTEAvbMUAQFENKNLPVIG ALASHRTEDG VSLFSPLERT MITLITCGDL QATTRIAVQG I RSWVVQQNS AIAI PSVEIN

KKIDELIYITD

TLAATP IKD

RSYAVSKLKP

LPIFKGLSNV

LSTVYTYK IT

ANDDMLKAFQ

EF130-3 (SEQ ID NO:487)

C

CGITAG CAAGTTGAAA CCAGCTGATG TCAAGAAAAA TCCI'TGAGCA CAGAAGCGGT GGTGCCATTG CGATACCAAG GTCGCTTTAT TAACTGGTC TATGCCTTGG CTAGTCATCG ACCAAAAAAG ACGAACTCAT ACTTCTCTAG AAAAAATCCA A.ATATGATTA CCITAATTAC GGAACATTAG CAGCAACGAC CAATTGGAGC AAAAAACTTT

TATGGCTCGT

GATGAAAGCC

TGTCGAAATT

CGGGACCATG

AACGGAAGAT

TI'ATATCACT

ACCAACCCGT

CTGTGGCGAT

GCCTATTAAA

AGCCGATTGG

GAAACAACGT

CAATTTGAA.A

AATITIGCCCA

AAAGAAGATC

GCCCTTTCCT

GATI'TATCTA

GTTGAGTTAA

TTACAAGCAA

GACGCCAACG

GTGGCT

TTGACT-TTGA

ACAAAAACTT

TTTTTAAAGG

AAGTCATGGG

TAT'ITTCACC

CT--TTTATAC

TGATGACGT

CGACGCGAAT

ACC-ATATGTI'

TTCAGTTGAG

ACCTGTGATT

AFI'GTCCAAT

GAAAAACAAT

'TI'AGAAAGA

ATACAAAATA

TCCTGGTCAA

TGCTGTTCA-A

GAAGGCTI'TC

EF130-4 (SEQ ID NO:488)

VSKLKP

ADVKKNMARE TTFDFDSVE.S LSTE-AVM(AQ FENKNLPVIG AIAIPSVEIN

LPIFKGLSNV

ALLTGAGTMK EDQVMGKNNY ALASHRTEDG VSLFSPLIERT KKDELIYITD

LSTVYTYKIT

SVEKIEPTRV ELIDDVPGQN MITLITCGDL QATTRIAVQG TLAATTPIKD ANDDMLKAFQ LEQKTL.ADWV A EF131-1 (SEQ ID NO:489)

TAGGCGCAGG

TGGCTITTTA

TTCT'TI'CA

CGCCGAACTG

CTI'GATCAAA

CAGGTTTTAG

CCAAACACAA

AA.AGCAATTG

ATTCAAGGTG

TTATCAGCAA

AATACCATTC

GCAGGAATGT

CACAAAACGG

AATTCAACAA

TAACGTAT

TAGTATGITI'

CTAGAGAT'C

ATAA'ITATGC

AA-ATI'CAAGA

TAAATAAAGG

GGTTTCAGAT

AAGAAGGTAA

CTGAGGATAT

TGCCTAATAA

AAGTCAATAA

TAGAGAAAAT

CTIGGTTTAAA

GTrATAAATG

GCGTAA.ACGA

GGCTGGTG

ACAAGTTAGT

GAA'ITTAACC

AAcAAATTAT ATATGGcT'TT

TGGCTCAATT

ACT'TACATA

TACGATTAGC

TATCGTTACC

AGGAAAATAC

GTATCAACGT

nAArTTTGGC

CATGCAAAGA

AT1'GGTCGTA

CAAGAATCGA

AAAGAAATAG

ATflGGI'CGG

GCCAATTTTG

CAAAA.ATCTT

GATACAAAAC

GATATGTTGA

GATGAAGAAA

AATTATTCCC

ACCTATCA.AG

'TCTATGAAA

AAAGACAiG

GTCTTATTT

AAGTGGTCrI'

TTGCACCAGA

CTTTGATCAT

AAAAGCAACA

TTACCACA-AC

TCGCTACGI'

ATATGACAAG

TTATTCAATT

CAGTAAATTT

AITTA'TI'AA

CCTTATTGGA

ACCA.AGTGCT

AGGAGTGAAT

AATAAAAACG

GGAAGAAGAT

TAGTGGCGAA

TAAAr-ATGAT

AGCCAACACG

CTTGATCTTA

TTATCCGCAA

TGGT'IrAAAG

TGTTAAACAA

TGTCCTTA

TAATCTTTAT

ACAGCCCAAT

CTCAATTCCT

GACAGAAGAT AATTCATATA 242 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

GCAGCTAG?1'

TATTGGTACT

TTATGGACGT

TTACG'ITTAC

AAGACAGGGG

GGTTCGTTGT

TTGCCCATGA

TACATcAATT

CTTCTCAGCA

ACGGCGTTGA

TCATATTGCT

TCCATGATGT

A'FTTGGGACT

AACGAGTCGA

AAGCTCTTAT

AGCGGGTCA.A

AACTAACTGT

AACCAATTTA

GGTAATGTGG

cATTTAGTTT

CATMGCGGCA

CAAGCCCTGG

GTGAATCCAG

ACTGTTAA-AT

ATATGACGAC

CCACCGCACT

TCTATGTTCA

T=rATTTTC

CGAAATACAA

TTTAA

AGGTGATG

TTTGCAAAAA

TGATAATTAT

AAAAGATATG

AGAATTAATT

EF131-2 (SEQ 10 NO:490) MRKRH AKRRHGGVNW LFIVCLLVVI GGSGYLIKTF FFTRDSQVSQ ESKVVLEEOR RSDNYANLTI( EIVAPDSGEL DQKIQETNYI GSALIIKDDQ VLVNKGYGFA

NFEKQQANTP

NTRFQIGSIQ KSFTTTLILK AIEEGKLTLD TKLATFYPQI QGAEDIT7SD MLNMTSGLKL SANPNNIVTD EEIIQFVKQN TIQVNKGKYN YSPVNFVLLA GMLIEK14YQRT

YQELFNNLYH

KTAGLKNFGF YETLLEQPNN STSYKWTEDN SYNQVLSIPA ASFA4EFGTG,

NVDMTTGDLY

WYLHQLTSGH LVSTALLQKL WTSSQQSSYH GGIVVHDNYL RLHGVEAGQQ

ALVLFSKDMK

TGVILLTNCV NPAKYKELIG SLFHDVTNLT VKF EF131-3 (SEQ ID NO:.491) a.

a a TTT AATAAAAACG TTCTTTI'TCA CTA( CGCCGAAGTG ATAj CTTrGATCAAA AAA' CAGGTTTTAG TA CCAAACACAA GGT AAAGCAATTG AAG A TTCAAGGTG CTG TTATCAGCAA TGC AATACCATTC AAG GCAGGAATGT TAG CACAAAACGG CTG AATTCAACAA GTT GCAGCTAGTT TTG TATTOCTACT TAC TTATGGACGT CTI TTACGTT'AC ACG AAGACAGGGG TCA GGTTCGTTGT TCC

~AGATTC

TTATCC

TTCAAGA

FTAAAGG

ITCAGAT

AGGTAA

AGGATAT

CTAATAA

TCAATAA

AGAAAAT

GTTTAAA

'ATAAATG

C CCATGA

ACAAGTTAGT

GAATTTAACG

AACAAATTAT

ATATGGCT

TGGCTCAATT

ACTITACA'ITA

TACGATTAGC

TATCGTTACC

AGGAAA.ATAC

GTATCAACGT

GAATTI'TGGC

GACAGAAGAT

ATTTGGGACT

CAAGAATCGA

AAAGAAATAG

ATI'GGTTCGG

GCCAATTTTG

CAAAAATCT

GATACAAAAC

GATATGTTGA

GATCAAGAA

AATrATTCCC

ACCTATCAAG

TTCTATGAAA

AATL'CATATA

GGTAATGTG

CAT

T

ITAGTIT

CATGGCGGCA

CAAGCCCTGG

GTGAATCCAG

ACTGITAAAT

AAGTGGTCT~r =rCACCAGA C ITGATCAT

AAAAGCAACA

TTACCACAAC

TCGCTACGTI'

ATATGACAAG

TTATTCAATTl

CAGTAAATTT

AATATTrAA

CCTTATTGGA

ACCAAGTGCT

ATATGACGAC

CCACCGCACT

TCTATGTTCA

T=IATmTC

CGAAATACAA

TT

GGAAGAAGAT

TAGTGGCGAA

TAAAGATGAT

AGCCAACACG

CTTGATCTTA

TTATCCGCALA

TGGTTTAAAG

TGTTAAACAA

TGTCCT'FTTA

TAATCTTTAT

ACAGCCCAAT

CTCAATTCCT

AGGTGATITG

TTTIGCAAAAA

TGATAATTAT

AAAAGATATG

AGAATTAAT'

:ATCAATT AACGAGTGGA CTCAGCA AAGCTCTTAT ~GCGTTGA AGCGGGTCAA LTATTGCT AACTAACTGT :ATGATGT AACCAATTTA EF131-4 (SEQ ID NO:492) LIKTF FFTRDSQVSQ ESKVVLEEDR RSDNYANLTK EIVAPDSGEL DQKIQETNYI NTRFQIGSIQ KSFTTTLILK AIEEGKLTLD SAMPNNIVTD EEIIQFVKQN TIQVNKGKYN KTAGLKNFGF YETLLEQPNN STSYKWTEDN WYLHQLTSGH LVSTALLQKL WTSSQQSSYH TGVILJTNCV NPAKYKELIG SLFHDVTNLT

GSALIIKDDQ

T(LATFYPQI

YSPVNFVLLA

SYNQVLS IPA

GGIYVHDNYL

VKF

VLVNKGYGFA

QGAEDITISD

GMLE<YYQRT

ASFAHEFGTG

RLHGVEAGQQ

NFEKQQANTP

MLNMTSGLKL

YQELFNNLYH

NvDmTTGDLY

ALVLFSKDMK

EF132-1 (SEQ ID NO:493) TA~~iTACCCAACAATTTAAAGAGGGTGTTAGGAAGA-GAT

GTGAGCGGAGTATCCTTAATG~-AGTACATGAAAAAAAAT

243 TABLE 1. N'ucleotide and Amino Acid Seqeuences of E. faecalis Genes.

GCTGAGAATAAGATGTCATTGTTACGTTATAATTGAAAAA,

ATTGArACACAGTATCGTACCTATTAGATCCCCACGATAGACC tTTGCCTGAAGATGTTCAAAAAACT mATCCGAACAGAGGAAACAAAGACTATTTTGCAGCGTATGGCATAcTATATACTTAGAGccTCAGAcT

GAA-=AGAACCAGTGTAATAAAAGTTATAGTAATTGAATG

TTGGAA-GTCrAATAAATCAAAAATTGTATTTGAATGATTT GAAAAGTAACAATGCTATCGAGTAAATGTGTCATAGA CrtAAA EF132-2 (SEQ ID NO:494) MMRKWVVVGSLMLIALF I FGACSTNSKDKDTVASNEKLIKVVTNS I LAD ITEN IAKDK I DLHS IVP IGKD PH EYEP LPEDVQKTSKADL IFYNGVNLXTGrNAWFTYKLVKXANKEEN(DYFAASDG

IDVIYLEGQSEKGKEDPHAWLNLENGII

YAXN IEKWLAEKDPDNKKFYKENLDKYI EKLDSLDKEAKSKFAS IPNDKKMIVTSEGCFKYFSKAYNVPSAY IWE INT EEEGTPDQ IK}{LVEKLRTTKVPSLFVES SVDDRPMKTVSKTI P IYSTIFTDS IAEKGQDGDSYYAMMKflWNLDK

IAE

GLSK.

EF132-3 (SEQ ID NO:495) ATATCAACCtTrCT

GTTCAAACMAACGTTATTTAACGGTATG~A

TGAGATC*GTTCAATATA*nTCGAAAAGAACAAATTTTCGAGG

TGCTGTTATATTGGGCGGGGAGGAAGAACCAGCTGTAATAAA

CGTTATAGTAATTCAATGTGG~&-AACTAATAAATTTAGA-

TCAAkGAATAALTGATTTGCAGACAACAATGTCATCATAA A*AAGTGTCATG.GAGTtATTTCCAACTTAGGCTCGTAATGG **AATTGCTGAAGGCCTrTCGAA-A EF132-4 (SEQ ID NO:496) CSTNSKDKDTVASNEKLKVVVTNS ILADITENIAKDKIDLHS IVP IGKDPHEYEPLPEDVQKTSKADL

IFYNGVNLXT

G-GNAWFTKLVKXANKEENKDYFAASDGI DVIYLEGQSEKGKEDPHAWLNLENGI IYAXNI EKWLAEKDPDNKKFYKEN *LDKY IEKLDSLDKEAKSKFAS I PNDKKMIVTSEGCFKYFS(AYiVP SAY IWE INTEEEGTPDQ IKHLVEKLRTTKVPS LFVESSVDDRPMKTVSKDTNI PIYSTIFTDSIAEKGQDGSYYAMKWNLDKIAErCLSK 9 9* S *9 S Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in Genlank and Derwent databases.

Query GenBank GenBank Ge ne Dcscription BLAST BLAST Access. No. Score P-Value EF002-2 gi12338759 (AF0 18073) periplasmic sorbi tol -binding protein; SmoE 113 3.6013-18 Rhodobacter EF003-2 gil 1552773 hypothetical [Escherichia coli] >gnlIDId 10 12634 hypothetical 278 1 .20E-53 EF003-2 gi12196996 lipoprotein homolog [Treponema palliduni] >giJ2 108234 29K 309 3.30E-44 EF003-2 gil46649 lipoprotein-28 precursor [Escherichia coli] >gi12905 10 263 9.20E-40 EF003-2 gi1148838 28 3kDa membrane protein [Haemophilus influenzae] 197 2. 10E-39 EF003-2 giJ1573614 28 kDa membrane protein (hIpA) [Haemopliilus influenzae] 197 7.80E-39 EF003-2 gi123 14748 (A.E000654) outer membrane protein [Helicobacter pylori] 263 4.60E'--37 EF003-2 gi13495 30 _lipoprotein [Pasteurella haemnolytica] >gill 50508 lipoprotein 189 4.i1OE-29 EF003-2 I lPID el 18435 similar to hypothetical proteins [Bacillus subtilis] 158 2.70E-26 EF003-2 giJ349532 lipoprotein [Pasteurella haemolytical >pirl1N075311N0753 outer 200 1.2013-25 EF003-2 gi11336657 lipoprotein [Bacillus subtilis] 182 2.70E-25 EF003-2 gnljPlDje233873 hypothetical protein [Bacillus subtilis] >gnljPlDjel 182900 186 1.3013-23 EF003-2 giJ294071 lipoprotein 3 [Pasteurella haemnolytica] 199 '6.60E-23 EF003-2 giJ34953 I lipoprotein [Pasteurella haemolytica] >pirIJN0752IJN0752 outer 198 1.30E-20 EF003-2 gil294070 __lipoprotein 2 [Pasteurella haemnolytica] 198 1.80E-20 EF005-2 giJ537235 Kenn Rudd identifies as gpmB [Escherichia coli] >gill 790856 127 6.20E- 12 EF006-2 gi1155277 3 hypothetical [Escherichia coli] >gnlPD~d 10 12634 hypothetical 255 1.40E-60 _29.4 1 0 OOe 0* 0* 0** 000 0 09 0 0 0 0*0 0 0 0 0S 0 0 0 00 0 0 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF006-2 giJ349532 lipoprotein [Pasteurella haemolytica] >pirjJN0753iINO753 outer 221 6.40E-49 EF006-2 gil23 14748 (AE000654) outer membrane protein [Ilelicobacter pylori] 283 2.70E-48 EF006-2 g11219699 6 lipoprotein homolog [Treponema pallidumi] >giJ2 108234 29K 267 4.4013-47 EF006-2 gn IPIDIel 18435 similar to hypothetical proteins [Bacillus subtilis] 359 1.80E-44 EF006-2 giJ3 4 95 3 1 lipoprotein [Pasteurella. haemolytical >pirj1N0752jfN0752 outer 218 3.8013-41 EF006-2 g11294071_ lipoprotein 3 [Pasteurella haemolytica] 220 2.30E-38 EF006-21 gil 146 6 49 lipoprotein-28 precursor [Escherichia coli] >gi12905 1 0 193 2.60E-38 EF006-2 giJ294070 lipoprotein 2 [Pasteurella haemolytical 218 1 .2013-36 EF006-2 gil 148838 28 kA membrane protein [Haemophilus influenzae] 112 8.50E-34 EF006-2 gil 1573 6 1 4 28 kDa membrane protein (hipA) [Haemophilus influenzae] I112 1.50E-"3 EF006-2 gil3495 30 lipoprotein [Pasteurella haemolytical >gill150508 lipoprotein 114 4.30E,-29 EF006-2 gi12940 69 lipoprotein 1 [Pasteurella haemolytical 114 1.3k0-27 EF006-2 gi1133 665 7 lipoprotein [Bacillus subtilis] 202 2.10E-26 EF006-2 gri1IPlDIe23387 hypothetical protein [Bacillus subtilis] >gnljPIDjeI 182900 200 6.5013-25 EF008-2 gif49301 7 endocarditis specific antigen [Enterococcus faecalis] 1590 2.7013-211I EF008-2 giJ3932 6 9 adhesion protein [Streptococcus pneumoniae] 986 1.8013-129 EF008-2 gil 1538 3 4 adhesin specific for salivary pellicle of dental surfaces 973 1.0013-127 EFOO8-2. gil 1575030 surface adhesin A precursor [Streptococcus pneumoniael 934 .2.90E-126 EF008-2 gi1153 826 adhesin B [Streptococcus sanguis] >pir1A435831A43583 adhesin 916 3.90E- 126 EF008-2 gill 1184932 jScbA [Streptococcus crista] 915 3.40E- 125 EF008-2 gill 1117994 Isurface antigen A variant precursor [Streptococcus neurnoniae] 917 15.60E- 124

S

S

S

0 S S S

S

S S S S S S S S S S *5 55 S Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF008-2 giJ3 10633 adhesin [Streptococcus gordonii] 891 6.OOE- 122 EF008-2 gn1lPIDe255529 lipoprotein [Staphylococcus epidermidis] 476 1.20E-99 EF008-2 giJ 1573330 adhesin B precursor (fim.A) [Haemophilus influenzae] 380 1.60E-68 EF008-2 giJ1245 464 YfeA [Yersinia pestis] >gi11 2 4546 4 YfeA [Yersinia pestis] 355 l,20E-64 EF008-2 giJ755O 7 5 periplasmic-binding protein [Synechocystis sp.] 321 1 .70E-62 18652 Mn EFOO8-2, g1133591 2 EwIA [Erysipelotluix rhusiopathiael 232 4.40E-42 EF008-2 guliPIDlel 18595 similar to ABC transporter (membrane protein) [Bacillus 204 4.10E-38 EF008-2 gi117779 3 3 TroA [Treponema pallidum] 181 2.4013-35 EFOO9-2 giJ34953 I lipoprotein [Pasteurella haemolytica] >pirlJN0752jJN0752 outer 391 4.OOE-64 EF009-2 giIl5527 73 hypothetical [Escherichia coli] >gnljPIDjd 10 12634 hypothetical 359 1.90E-63 29.4 EF009-2 gi129 4070 lipoprotein 2 [Pasteurella haenolytica] 391 6.40E-63 EF009-2 gi3 4953 2 lipoprotein [Pasteurella haemolytica] >pirIlu'.u 75.IJINU 0- o uter a Ou EF009-2 gi1148 838 28 3kDa membrane protein (Haemnophilus influenzae] 286 5__60E_60 EF009-2 gij 1573614 28 kDa membrane protein (hIpA) [Haenoph ilus influenzae] 286 .7.60E-60 EF0Q9-2 gi129 4 069 lipoprotein 1 [Pasteurelia haenolytical 122 4.70E-59 EF009-2 giJ146 649 lipoprotein-2 8 precursor [Eschericbia coli] >g12905 10 326 12.20E-58 EF009-2 giJ349 530 lipoprotein [Pasteurella haemol)tica] >gillSOSO 8 lipoprotein 239 _I7.80E-57 EF009-2 giJ29 4 0 7 1 lipoprotein 3 [Pasteurella haemolytica] 344 4.90E-56 EF0O9-2j gi123 14748 I(AE000654) outer membrane protein [Helicobacter pylori] 319 4.20E-53 I r onma nalidiiml >i12108234 29K 312 2.601341 EF00U9-2 1,4 1 VOYYD protein LLIIJ A

__I

A a a a a a a a a a a a a. a a a a a a a *a a a a. .a a Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF009-2 gil 1336657 lipoprotein [Bacillus subtilis] 234 4.0013-32 EF009-2 gn1lPIDle233873 hypothetical protein [Bacillus subtilis] >gnlIPlDI 182900 242 1.40E3-31 EF009-2 gnliPlDjel 18435 similar to hypothetical proteins [Bacillus subtilis] 102 6.80E-22 EFOl 11-2 gnIPlD~dl096 ferric anguibactin-binding protein precusor FatB of V. 579 3.1013-98 F0 11-2 gnlPIDjdIO096 ferric anguibactin-binding protein precusor Fatl3 of V. 579 3.1013-98 EFO 11-2 gnljPlDjel85374 ceuE gene product [Campylobacter colil 284 1.30E3-89 EFOI 1-2 gnljPIDIl537 4 ceuE gene product [Campylobacter ccli] 284 1.30E3-89 EFOl 11-2 gi1 150756 40 kDa protein [Plasmid piMI] >pirlA29928lA29928 222 2.80E-52 membrane-associated EFOl 1-2 gil 15075 6 40 kDa protein [Plasmid pJMl] >pirlA299281A29928 222 2.80E-42 membrane-associated E3F012-2 giJ3O9662 pheromone binding protein [Plasmid pCF 10] 266 8.70E-1 116 >pirjB53309IB53309_____ EF012-2 giJ3882 69 traC [Plasinid pAD I) >pirlA5331I0lA533 10 pheromone cADi1 252 1.101-109 ~~binding EFOI12-2 gnljPlDjdlOl 18 TRAC [Enterococcus faecalis] 281 3.60E3-103 EF0 12-2 gn]IP]Dld 10065 TraC [Enterococcus faecalis] 277 2.3013-102 F0 12-2 giJ3 12940 threonine kinase [Streptococcus equisimilis] 227 1.90E3-67 >pirIS28 153IS281 53 EF012-21 giJ48808 jdciA-E [Bacillus subtilis] 228 11.70E-46 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EFO 12-2 pirISI 665 1 IS166 dciA.E protein Bacillus subtilis 228 1.OOE-45 EFOl12-2 gnlIDel 18149 (AJ00257 1) DppE [Bacillus subtilis] >gnilIPIDIel 183316 228 3.80E-45 EF012-2 giJ40005 OppA gene product [Bacillus subtilis] 281 3.90E-44 EFO 12-2 giJ1 4 360 3 sporulation protein [Bacillus subtilis] >gn1 PIDe e1183 163 281 7.70E-44 EFOl12-2 gnllPlDldl0156 Periplasmic oligopeptide-binding protein precursor. 152 2.20E-43 3 EF012-2 giI157 46 79 oligopeptide binding protein (oppA) [Haemophilus influenzae] 178 2.20E-42 EFOl12-2 giJ47802 Opp A (AA 1-542) [Salmonella typhimuriumi >giJ478O8 128 1.OOE-37 EFOl12-2 giJ88 2 55O ORFJf535 tEscherichia coli] >gi1 1789397 (AE000384) [535; 228 5.3013-36 This 535 aa EF014-2 pirID7007OlD7O transcriptional regulator homolog ywtF Bacillus subtilis 101 1.40E-J7 0_ EFO 14 2 gnjPlDe 116988 capsular polysaccharide synthesis protein (Streptococcus 121 9.50E-.27 EFO 14-2 gi1280 4 769 (AF030373) putative regulatory protein [Streptococcus 121 9.50E-27 EFO 14-2 gnllPIlDe28 9126 1unknown [Streptococcus pneumoniae] 121 l.OO13-24 EFOI14-2 giJ2267239 ORFI [Staphylococcus epidermidis] 234 .1.50E-24 EFOI14-2 gi148527 5 _putative regulatory protein [Streptococcus pneunoiniael 121 3.90E-24 EF014-2 giJ280 4 7 3 5 (AF030367) putative regulatory protein [Streptococcus 121 3.90E-24 _____pneumoniaej EF014-21 gi1280 4 747 (AF030369) putative regulatory protein [Streptococcus 1.21 3.90E-24 pneumnoniae 9000 Oee 9 SO 0~ 9 0 e e S.

CO C e 0 Se 0 060 0* 0 S Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EFOl14-2 git 1762327 putative transcriptional regulator [Bacillus subtilis] 185 2.80E-22 EF014-2 -gi114315 6 membrane bound protein [Bacillus subtilis) >gn1IP1Dle1 184471 116 l.lO13-21 EF014-2 gnIlPIDIdlO 189 membrane bound protein LytR [Synechocystis sp.] 113 6.20E-20 EFOI14-2 gi1127 6 874 jEpsA [Streptococcus thermophilus] 103 4.OOE- 17 SICAAI r 1'2 C EF016-2 EF016-2 EF016-2 t~r~t~I~t I C ;lmf% w4d API' trans orter (11inding protein I gn Piiii~ IfJU II U a& "ALL -SS ar inine binding protein [Ecei~ oi gl674at 121 .0E3 giJ4U934 I u- I gn]IP1DId10 152 Argiinebiningperplamicprotein 2 precursor [Escherichi a 14.ISUL-.3 1 I I I EFOI16-2 gi1687 652 j EFOl16-2 giJ26504I0 FlIN [Escherichia coli] >gnlPlD~dt 10 16464 FINY protein 10 5.70E-31 (AEOO 1090) glutamine ABC transporter, periplasmic glutamine- 122 3.30E-29 104nding~2 EFQ 16-2 EF016-2 EF016-2 gi1l 64 90 35 ai1157 4634 g1415 6 9

I

high-affinity periplasmic glutamine binding protein [Salmonell I I 2.50E-27 EF016-2 jgnhiPIDtd 1015: GlnH precursor (AA -22 to 226) [Escherichia coli] 106 4.70E-27 >gnljPIDIdlO152SO____ 2 Arginine-binding periplasmic protein I precursor [Escherichia 109 .3.70E-26 artl [Escherichia coli] giJ7 6979 I arti [Escherichia coli] 127 2.30E-25 9 homologous to Gin-binding periplasmic proteins [Bacillus 117 8.50E-24 EFOI16-2 EFO 16-2 7 gil76979 1 gn.l1PIDId1 008 EFOI16-21 giI1541 25 'J protein [Salmonella typhimurium] >gi4771 8 reading frame 1,18 2.10E-23 0 0 0 0 *0 .i ~0* *0 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

hisJ EF016-2 gnIIPlDjdl0l68 HISTIDINE-BINDING PERIPLASMIC PROTEIN 117 4.50E-23 8 PRECURSOR

(HBP).

EFO 16-2. g!1 1166636 histidine-binding perilasmic protein HisJ [Escherichia coli] 117 6.60E-23 EF017-2 giJ38 8269 traC [Plasmid pADI]) >pirjA533101A533l10 pheromone cADI 421 4.50E-1281 ~binding EFO17-2 gn1jPIDjIl 8 TRAC [Enterococcufs faecalis] 417 5.1013-124 EF017-2 gnljPIDjd1006S TraC [Enterococcus faecalis] 414 4.40E-123 EFO 17-2 giJ309 662 pheromone binding protein [Plasmid pCF 10] 415 2.40E-1 19 _____________>pirjB53309IB53309.k EFO17-2 giI40005 OppA gene product [Bacillus subtilis] 294 6.2OEA82 EFO 17-2 gil 14360 3 sporulation protein [Bacillus subtilis] >gnlIP1Djel 183163 290 2.80E-79 EF017-2 giJ 3 l 2 94 0 threonine kinase [Streptococcus equisimilis] 241 2.4013-71 ~>pirIS281I53IS28 153____ EF017-2 giJ488OS dciAE [Bacillus subtilis] 270 1.I1OE-61 EFOI17-2 gnIIPIDel181 49 (MJ00257 1) DppE [Bacillus subtilis] >gn]IP1Dle1 183316 270 .150E-61 EFO 17-2 oirIS 1665l1IS 16 6 dciAE protein- Bacillus subtilis 270 3.10E-60 EFO 17-2 giJ3049 5 j periplasmic oligopeptide binding protein [Escherichia coli] 171 2.60E-57 EF017- !~41l oligopeptide binding protein precursor [Escherichia colil 171 8.7013-56 j 1)9 J In JI..J IEF017-2 Opp A (AA 1-542) [Salmonella typfimunumJ >9g'if ,a'J6 precursor

C

a a Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EFOI17-2 giJ88255O ORFJf535 [Escherichia coli] >gil 178 93 97 (AE000384) f535; 135 5.50E-52 This 535 EFOl17-2 gil 1574679 oligopeptide binding protein (oppA) [Haeinophilus influenzae] 168 2.90E-43 EFO 19-21 giJ43845 8 liely N-terminal signal sequence; mature protein probably 104 .2.30E-17 EF021-2 gnlIDje3I1492 unknown [Bacillus subtilis] >gnlPD~e1 184232 similar to ABC 317 2.5013-103 EF021-2 bbs1173803 CD4+ T cell- stimulating antigen [Listeria mnonocyto genes, 476 2.80E-81 E0- 167, EFO21-2 gil 5 8 1 8 09 tmbC gene product [Treponema pallidumi >pirIA435951A43595 152 3.20E-71 membrane____ EF021-2 giJ26882 80 (A.E001143) basic membrane protein C (brnpC) [Borrelia 10 1 5.50E-27 EF021-2 gnlIDIel 17283 membrane protein A [Borrelia garinli]j 142 6.50E3-h EF021-2 gntljPIDlel 17283 membrane rotein A [Borrelia burgdorfen]l 141 9.20E-22 EF021-2 gnljItDjeI 17283 membrane protein A [Borrelia burgdor-feriJ >giIS 16592 141 9.20E-22 membrane EF021-2 gnljIlDje 17283 bnipA(239,ORF 1) [Borrelia burgdorferiii 141 1 .70E-21I EF021-2 giJ508421 antigen P39 [Borrelia burgdorferi] >gil 2 68 82 8 I (AEO0I 143) 141 1 .7012-21 basic EF021-2 gi11753 22 5 jBmpA protein [Borrelia burgdorferil 141 2.70E-20 EF021-2 gn]IP1De 11728 2 membrane protein A [Borrelia afzeiilj 141 8.60E-20 EF021-2 gnljPIDjIl728 3 Imembrane protein A [Borrelia afzelii] 141 8.6013-20 A 0 141 ~Af EF021-2 EF021-2 ,gn1jPIDje 17283 gnIjPIDje 17282 membrane protein A[Borrelia afzeliiJ bmpA(p39,OR.F 1) [Borrelia burgdorferi] J 141 1.50E-19 I 1.50E-19

I

I

I II

I

I

I

I I

I

Table 2. Closest matching sequences between tile polypeptides of the present invention and sequences in GenBank and Derwent, databases.

EF022-2 giJ1 2 9 40 threonine kinase [Streptococcus equisimilis] 324 5.90E-66 1531S28153_________ EF022-2 giJ3096 6 2 pheromone binding protein [Plasmid pCF 10) 307 5.60E-60 B53 3091B53309_ EF022-2 gnljPIDjdl0l 118 TRLAC [Enterococcus faecalis] 301 4.80E-59 EF022-2 gnIPIDeIel18149 (AJ 0571) DppE [Bacillus subtilis] >gntIPIDIe 1l8 33 1 6 170 5.LOE-59 EF022-2 gi14880 8 dciAE [Bacillus subtilis] 170 5.20E-59 EF022-2 gnlP1D~d1 0065 TraC [Enterococcus faecalis] 299 2.80E-58 EF022-2 pirI1665115l66 dciAE protein Bacillus subtilis 170 1.60E-57 EF022-2 giJ38 8269 traC [Plasmid pADI >pirIA533 I OA533l10 pheromone cADI1 290 2.70E753 ____binding EF022-2 giJ4 000 5 OppA gene product [Bacillus subtilis] 154 7.30E-48 EF022-2 gi11 43 6 0 3 sporulation protein [Bacillus subtilis] lfPIDjeI 183163 154 3. 1OE-47 EF022-2 gi2688227 (AEO 1139) oligopeptide ABC transporter, periplasmic 215 l.OOE-36 EF022-2 giJ228 1458 (AF000366) oligopeptide permease homolog All [Borrelia 215 l.OOE-36 EF022-2 gif 304925 Periplasmic oligopeptide binding protein Escherichia coli] 131 1 I.30E-35 EF022-2 gi114701 4 oligopeptide binding protein- precursor [Escherichia coli] 131 1 .80E-34 EF022-2 giJ47 8 02 Opp A (AAI-542) [Salmonella typhimurium] >giJ4780 8 138 4.90E-34 precursor __231 EF023-21 gi3 0 9 6 2 lpheromone binding protein [Plasmid pCF 10] 3 .7E6 e

S

Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in Gen~ank and Derwent databases.

-I>pifB533091B53309

III

EF023-2 EF023-2 EF023-2 EF023-2 EF023-2

FEF

0 2 3 2 EF023-2 EF023-2 EF023-2 EF023-2 giJ3 88269 gnl11PIDIdI01 18 gnI IPIDId 10065 giJ4 8808 gnjPlD le1 18149 pirS 16651 IS 166 giJ 40 005 gil 143603 giJ47802 giJ268 82 7 giJ2 28 l 4 5 8 traC [Plasinid pADI] >pir1A533lOlA53310 pheromone cADI binding TRAC [Enterococcus faecalis) TraC [Enterococcus faecalis] dciAE [Bacillus subtilis] (AJ002571) DppE [Bacillus subtilis] >gntPDlel 183316 dciAE protein Bacillus subtilis, OppA gene product [Bacillus subtilis] sporulation protein [Bacillus subtilis] >gnIPIDIe 1183163 Opp A (AAI-542) [Salmonella typhimuriumi >gil47 8 08 precursor (AEQO 1139) oligopeptide ABC transporter, periplasmic (AF000366) oligopeptide permease homolog All [Borrelia burgdorferil OREfJ535 lEscherichia colil] >gi117 8 9 397 (AEOOO3 84) f535; This 535 aa periplasmic oligopeptide binding protein [Escherichia coli] oligopeptide binding protein precursor [Escherichia coli] (AF005097) unknown [Lactococcus lactis] pheromone binding protein [Plasnmid pCF 226 1 OOE-58 226 4.40E-58 157 157 137 133 135 187 187 1 .20E-57 3.80E3-56 2.30E-53 6.90E- 3 2.0013-41 9.4013-41 I 1.90E3-40 155 11l'3013-38 E F 0 2 3 -2-rj i8 8 2-55 EF023-2 EF023-2 EF026-2 EF027-2 giJ3 04925 gi1 147014_ gil 235 24 82 giJ3O9 662 130 130 141 198 9.0013-37 3;70E3-34 1. 1OE-23 6.2013-71 6 669 6* 6 6* 3 6** 6 6

I

6 66 6 666 6 6 6 1 66 6* 6 *6 61 6 I It *6 66 #6 6~ 6 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

T F 11 202 EF027-2 gnljPIDldlO065 ITraC [Enterococcus faecalis] 202 1 .50E-68 I i i EF027-2 EF027-2 EF027-2 EF027-2 EF027-2 EF027-2 EF027-2 EF02 7-2 EF027-2 EF027-2 gn.1IPIDjdlOl 118 r,

A

TRAC [Enterococcus faecalis] traC [Plasmid pAD I] >pirIA533lIOIA533l10 pheromone cAD I binding 213__ j8.30E-68

I

gillDle 18149 (AJ00257 1) DpE[ailssbiis giII~l131 r 222 AlO-4 giJ48 808 pirIS 16651 IS 1 66 giI4 000 5 gi1143 6 0 3 gif3l12940 gi12688 227 gil2 2 8 1458 dciAE [Bacillus subtilis] dciAE protein Bacillus subtilis OppA gene product [Bacllus sutilis] 1222 1 LlOE-39 TJ 25 sporulation protein [Bacillus subtilisi >gnlPlDIel 183163 threonine kinase [Streptococcus equisimilis] >PirIS28 153 1S28 153 (AFO0l 139)oligopeptide ABC transporter, periplasmic (AF000366) oligopeptide permease homolog All [Borrelia ~burgdorferil (AF000948) OppAJV [Borrelia burgdorferi] >gi12 68 989

I

(AE0007 92) ~oligopeptide binding protein (oppA) [-aemophilus influenzae] B. subtilis alkaline phosphatase iIIA; P 19405 secretory 247 233 131 131 4IE-39 5.8OE-3 9 8.90E-,33 2.40E-24 2 .40E-24 117 1,OE2 EF027-2 1 gij2 2 8 1468 EF027-2 EF028-2 IEF028-2 gil 1574679 gnlfIPlDId 10204 7 pirIBl3 90961B39 130 996 3.50E-20 3.6013-131I alkaline phosphatase (EC 3.1.3. 1) 111 prcursor Bacillus 9 982 12.90E-129

I..

I a *1 II I I I I C I I

I

I. I I Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF028-2 EF029-2 EF028-2 EF028-2 EF028-2 EF028-21 EF028-2 EF028-2 EF02 8-2 EF028-2 EF028-2 EF028-2 EF028-2 0 gi147 0383 gi114 3324 gill 47243 gi11 4 7237 gi11 47239 giJ14724 I gi11.27 7 127 gi1147 229 g-181 8 851 gil 147245 gi1147 23 1 giJ1 4 7 235 gill 0 1 60 10 alkaline phosphatase A [Bacillus subtilis >gIIlel 182942 AkPase I [Bacillus lichenifon-nis] >pirlA44828lA4482 8 alkaline alkaline phosphatase precursor (EC 3.1.3. 1) [Escherichia coliLalkaline phosphatase precursor (EC 3.1.3. 1) Escherichia colil alkaline phosphatase precursor (EC 3.1.3. 1) [Escherichia colil alkaline phosphatase precursor (EC 3.1.3. 1) [Escherichia colil phoA gene product [Cloning vector pFW..phoAl >gil 12 77 130 phoA gene alkaline phosphatase precursor (EC 3.1.3. 1) [Escherichiacoli] alkaline phosphatase [synthetic construct] alkaline phosphatase (phoA) (EC 3.1.3. 1) [Escherichia fergusonii) alkaline phosphatase precursor (EC 3.1.3. 1) [Escherichia, coli] alkaline phosphatase precursor (EC 3.1.3.1)1[Escherichia coli] alkaline phosphatase with N-terminal PeIB-leader and Cterminal YncB [Bacillus subtilisi >gnillPlDlel 183421 similar to niicrococcal similar to hypothetical proteins [Bacillus subtilis] nuclease [Staphylococcus aureus] >pirIAOO790INCSAF icrococcal thernionuclease [Stahlococcus hicus] 803 184 183 178 178 178 174 178 174 177 174 174 174 4.8013-119 3 .OOE-54 8.30E-54 4.40E-53 -4.40E-53 4 .40E-53 4.90E-53 8.40E-53 IOE-52 1.20E-5,2 1 .6013-52 1 .60E-52 1 .60E-52 257 3;50E-55 EF029-2 giIl7501 2 6 EF029-2 EF029-2 gnljPIDje 1183 60 giJ67 3492 263 320 7.80E-53 2.20E-39 EF029-2 i gi5 32653 15 5 ]19.I1OE-39 9* 9.

4** 9 9 9 9 9 9 9.

9 9 99. 9 *9 9. 9 9 9 9ta* 9. 9 9* 9* 9 9 9 9 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in Genl~ank and Derwent databases.

I EF029-21 EFO30-2 EF030-2 EF03-2 EFO30-2 EFO30-2 rEF030.2

I

gIJ47 146 thermonuclease [Staphylococcus intermediusi >iflS26079IS260 7 9 gi48808 dciAE [Bacillus subtilisl mnlPI~ell 8 l 4 9 AJ002571) Dp2E fBacillus Subtilis] >gn1PlDje1183316 S,66 6 -51 S166 dciAE protein Bacillus subtilis giJ30 9662 pheromone binding protein [Plasmid pCF 101 ir B533091 353309 gnlIPIDjdl~l 18 TRAC [EnterococcUS faecalis) gn.1PIDldlOO 6 TraC [Enterococcus faecalis] 149 149 149 227 1.l1OE-66 1 .50E-66 5.90E-66 1 7.4013-52 2 37 F7.40E-52 233 9.70E-5Sl 22 9 3 .OO0E_48 giJ38 8269 EF030-2 gi(3 12940 EF030-2 giJ4 7802 traC [Pla smid pADI lI >pir1A533101A 53 3 l 0 pheromone cADI binding threonine kinase [Streptococcus equisimilis] >pirIS2S 153IS28153 Opp A (AAl-542) [Salmonella typhimuriufli >gil 4 780 8 277 3.OOE-45 125 8g.50E-34 125-3 recursor EFO3O-2reur 2682 AQl19 hoetide ABC trasporter, Renplasinic EF030-2 gi1222 8 1458 (AF000366) oligopeptepreaehmogM[orei b u r d o r fe ri t l s EF030-2 i 40005 0 A ene Rroduct [Bacilluis subtis EF030-2 143603 s orulation protein B acillus subtilisl ltPIDj IeI 183163 E F030-2 giJ228l 4 68 (AF000948) OppAIV [Borr elia burgdorferil >gi12 68 989 l 00OO07 92) S211 211 148 144 210 4.80E-31I 1 .20E-30 4.80E-30 2.IE-2

S

S S S S S S S *5 S. S S S S S S S S S S S. S.

S

Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Der-went d .atabases.

EF030-2 EF033-2I EF033- EF033-2 EF03 6-2 EF036-2 giJ157 4 679 gnljPJDjI 18439 pirjS54437JS5 44 g1619 623 gnlPIDIdl 0102 2_ gi126 22858 EF036-2 jgiJ26 22859 EF036-2 1 gi12 688 115 oligopeptide bndig potein (oppA) [H-aemophilus influenzae] similar to iron-binding protein [Bacillus subtilis] hemnin binding protein Yersinia enterocolitica hemin binding protein [Yersinia enterocolitica]_ ORF 108 IBacillus subtilis) >gnljP1DeII857 6 6 alternate gene (AE000929) phosphate-binding protein PstS (Methanobacteflum (AE000929) phosphate-binding protein PstS homolog [Methanobacteflum.

(AEOO 132) phosphate ABC transporter, periplasmic tphosphate-binding (AF005097) unknown [Lactococcus lactis] hypothetical protein [Esherichia coli] >gil78651 1 (AEOOO 139) 2-5A-de endent RNase [Mus musculus] >;pirtB45771lB3 4 5 7 7 l G9a [Homo sapiens] >pirS30385IS 3 0 3 8 5 G9a protein human erythroid aniyrin [Mus musculus) >pirS3777 S3 77 7 1 ankyrin, ankyrin [Mus musculus] >pir114950211 49 502 ankyrin mouse alt. anrn (variant 2.2) [Homo sapiens] ankyrin [Homo sapiens] ankyrin [Homo sapiens] ankyrin 1, erythrocyte splice formn 2 human 148 164 108 108 544 183 1.40E-45 158 12.40E-41 11-7 1IOE-12 6 OOE-29I 2.60E- 14 1.40E-1 I 2.OOE-1 1 1 .20E-96 EF037-2 EF040-2 2 EF040-2 EF040-2 EF040-2 EF040-2 EF040-2 EFO4O-2.

EF040-2 giJ23 52482 giJ 1657516 giJ293 2 65 61~ 2 8786 5 gil 31 1817 giJ19 1940 gil7 477 1 0 giV]178646 giI18 45 26 5 pirlA3 5049IA3 5 141 208 105 143 119 119 120 120 10 120 1.l08-23 1,.90E-29 i.OOE- 17 8 .30E=-14 -4.80E- 13 4-.90E- 13 1.50E-12 1.80E-12 I1.80E-12 I.8E-1 L 0 a 4 9* 4 q 4 4* 49 S 9 9.

9 9~ S 9 a 9 5 9

S

Table 2. Closest matching sequences between the polypeptides of the present ivnonadsquences in GenBank and Derwent databases.

a a 9 9* p.

9..

9 9* 9 a 9* a a a a a a a a.

a a S a S S. *a Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

1EF041-2 giJ147 0 1 4 oligopeptide binding protein precursor [Escherichia coli] 160 1 .50E-28 EF041-2 giJ22 5328 6 (AF005657) plasminogen binding protein [Borrelia burgdorferiL 134 5.0011-27 tEF045-2 giJ3O 8 85 4 oligopeptide binding protein [Lactococcus lactisl 43 3.21312 I L I EF045-1 EF045-2 EF045-2 EF048-2 EF04 8-2 EF048-2 EF048-2 EF048-2 EF048-2 EF04 8-2 EF049-2 gi)49 5 1 8 1 giJ 6 7 79 45 giJ293 0 14 gil 1574060 dbjllABOO 1498 2_ gi146 6 7l 7 gil 46 00 6 gil 7 3 102 _gil2 182530 gi15 7 2999 gi1149 58 1 oligopeptide binding protein [Lactococcus lactis] 426 9.70E- 124 AppA [Bacillus subtilis] >gnlPIDIel 183158 oligopeptide ABC 154 2.30E-31 peptide-binding protein [Lactococcus lactis] 159 2.40E- 14 >pirj B47098B47098____ hypothetical [Haemophilus influenzae] >pir116416411 64 164 250 2.30E-41 (ABOG 1488) SIMILAR TO C4-DICARBOXYLATE- 208 3.60E-34 BINDING PERIPLASMIC______ No definition line found [Escherichia coli] >gill 79 000 4 199 1.3013-30 penipiasm-ic C4-dicarboxylate binding-protein [Rhodobacter capsulatus] hypothetical [1amophilus influenzae] >piilH64 14311-64143 (AE000085) Y4mM [Rhizobium s NGR234] hypothetical [Haemophilus influenzael >pirIE64 141 I E64 141 maturation protein [Lactobacillus paracasei] >pirIA448581A44858 ORF (AA 1 to 299) [Lactococcus lactis cremoris) >pirlS0 8 0 83 1 S08083 ~maturation protein [Lactococcus lactis] >gi62 3 055 proteinase 162 11.405-25 2_4 4 114 116 241 239 3.80 5.60E- 18 5:9013- 2.40E-55 1 .O13-54 I6.20E-54 EF049-2 giJ414 32 0 7 ~EF049-2 giJ4321 98 9 90 0* 0 099 9. 0 4 9 9*.

:0 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GerLBank and Derwent databases.

EF049-2 giJ4728 35 ORFI [Lactococcus lactis cremoris] 241 11.5013-53 EF049-2 giJ39782 33kDa lipoprotein [Bacillus subtilis] >gnlIPlDje325 181 33kDa 128 8.90E-40 EF051-2 gnlPIDjdl10l114 molybdate-binding periplasmic protein [Synechocystis sp.] 173 3.2013-50 2 EF051 2 IgnlIPID~eI 18602 alternate gene name; yvsD; similar-to molybdate-binding 314 5.90E-50 1-2 gil 1574546 EF051-2 gi150 44 9 8 EF051-2 01g1 48 9 39 EF054-2 EF054-2 EF054-2 _901505 56 gn1IPlDle23657 I giJ457 38 lsg locus hypothetical lHaemopflhlus inhiuenzacj >pirlA641I751A64 175 periplasmic, inolybdate-binding protein [Escherichia coli] >-gill 147 817 ORF 8 [Haemnophilus influenzae] >pirIS27S 83IS27583 hypothetical surface protein [Plasmid pCF 101 >pirIA4l8261A4]826 probable cell wall anchoring signal [Enterococcus faecalis] ORFC [Enterococcus faecalis] >pirIJHO2O4IJHO2O4 hypothetical 30.5K orf iota [Str~eptococcus pyogenes] >pirIS681I25IS4509 1 hypothetical sporozoite surface protein [Plasmodium yoeli) >pirIA45559IA45S 59 PbTRAP fPlasmodium bergheil zinc finger protein [Mus inusculus] >gnlfP1Dj225 6 88 zinc IgG and IgE immnunoreactive antigen recognized by sera from patients 1490 515 372 1.80E-192.

1 .60E-58 148 1.40E-30 150 I8.IOE-28 EF054-21 giJ496S 2

O

362 1I.30E-43 286 14.-30E3-33 EF054-2 EF04- EF054-2 EF054-2 giJI6 06 9 3 7 gi1181 35 23 gn1jPlDje225 6 87 giJ2290 394 305 246 242 1.30E3-32 3.60E3-26 1.40E3-25 4 4..

9** 4 9.

4* 9 9 4 9 44 4 9 *4 9. 9 4 9 9 9 9 99 99 9* 4 9 9 9994 99 9 9 Table 2. Closest mnatching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF054-2 EF054-2 EF054-2 EF054-2 EF054-2 gi 9 3 92 gi46523 pirnS 153301jFCS 0 [1620100 *i636869 Pro- and Glu-rich, PENPEV (l0x); similar to Streptococcus

B

NF-M c-terminuS GCallus gallus) NF-M protein [Gallus gallus] >pirjS 1 57621S 15762 neurotilament triplet TATA-box like sequence (Usi 11) [Human herpesvinis 1) >giJ29193 18 cell wall anchoring signal [EnterocoCCUS faecalis] sufaerotein [Plasmid pCFI10 >pir A4 1826 IA4 1826 oal ORFC [EnterococCus faecalis] >pirlJ H0204 1JH0204 232 228 210 222 222 2 .80E-23 1 .OE3-22 3.I1OE-21 6.90E-2 1 8.SOFE-21 1T94-4. IOE- 19 EF054-2 1 gi17 578 67 EF059-2 EF059-2 gnlIPlD~e23657

I

6150556 I giJ4573 8 418 1366 5 .6013-95 3 .70B-87 9,30E-50 EF059-2 EF059-2 EF059-2 EF059-2 9-2 hypothetical 30.5K gi149 6520 orf iota [Streptococcus pyogenes] >pirIS6 8 125IS4509

I

yotetical gill1 6 06 93 sporozoite surface protein [Plasmnodiumn yoeliil >pirIA4S559IA4555 9 g1813523 PbTRAP IPlasmodium berghei gi122 9 0394 lgG and IgE inunnoreactive antigen recognized by sera from pati ents gi12 290 392 IgG and IgE immunoreactive antigen recognized by sera from watients 367 5.90E-44 1 344 1-.IE-38 2-95 251 251 =2.50OE-32 3 .OOE-29 3E 0- 29 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF059-2 gil 1620100 Pro- and Glu-rich, PENPEV (IlOX); similar to Streptococcus B 253 6.40E-27 EF059-2 gif 46521 Fc receptor [Streptococcus agalactiae] >pirlA602341A60234 IgA 197 2.70E-26 Fc EF059-2 gi4 6523 B3 antigen [Streptococcus agalactiael 232 9.30E-26 EF059-2 pirISl533OIECS IgA Fc receptor precursor Streptococcus agalactiae 232 19.30E-26 0 EF059-2 gn~lPIDDle225687 zinc finger protein [Mus musculus] >gnIjPlDje225 6 88 zinc 234 1 .4E-22 EF059-2 gil42 5356 zona pellucida protein [Pseudopleuronectes aniericanus] 229 1 OOE-21 EF059-2 gi(4577 6 9 Collagen [Bomrbyx mori] >pirlS42886IS4288 6 collagen 209 7.60E- 19 silkwormn EF061-2 gnlPIDe236571 cell wall anchoring signal [Enterococcus aecalis] 925 _18.1E-1 18 EF061-2 IL05 surface protein [Plasmdd pCFIO] >pirlA41826lA41826 probablel 350 11.50E-107 I-)n 1I I AAEZ'.CQ EF061-2 giJ496520 EF061-2 giJ4573 8 EF061-2 gil 18135 23 EF061-2 gil 160 693 EF061-2 giJ 636 86 EF061-2 giJ6 36 89 EFO6I-2 giJ2290 392 orf iota [Streptococcus pyogenes] >pirIS61SbIji43u0i I hypothetical ORFO [Enterococcus faecalis] >pirlJHO204[1H020 4 hypothetical 30.5K PbTRAP [Plasmodium berghei] sporozoite surface protein [Plasmo dium yoelii] >pirIA455591A455 5 9 INF-M c-terminus [Gallus gallus] INF-M protein [Gallus gallusi >pirIS I57621Sl15 76 2 6.40E-50 322 263 1 OOE-26 241 -9:.O0E-25 232 2. 1OE-22 232 2.60E-22 176 12.4013-21 neurofilament, triplet lIgG and IgE imunoreactive antigen recognized by sera from S S S *5 S Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF061-2 gil 16201 00 Pro- and Glu-rich, PENPEV (lOx); similar to Streptococcus B 165 2.70E-20 EF061-2 gnljPID~e22568 7 zinc finger protein [Mus musculus] >gnljPIDje22568 8 zinc 197 7.80E- 19 EF061-2 gil 160 355 interspersed repeat antigen [Plasmodium falciparum] 199 8.20E- 18 EF061-2 giJ410750 interspersed repeat antigen [Plasmodium falciparum] 199 8.90E-18 EF061-2 giJ22903 8 8 IgG and IgE iminunoreactive antigen recognized by sera from 182 1.40E- 17 ________patients EF061-2 giJ2290 394 lgG and IgE inmunoreactive antigen recognized by sera from 180 2.80E- 17 patients EF062-2 gi470 49 asal gene product (AA 1-1296) [Enterococcus faecalis] 3716 0 EF062-21 giJ4332 4 aggregation substance (ASPI1) [Enterococcus faecalis] 4003 0 EF062-21 giI2 109266 aggregation substance [Enterococcus faeciuin] 5523 0 A~ 141 41Q A EF062-2 EF062-2 EF062-2 gil 150555 gil 100973 giJ47 248 aggregation substance [Pl'asmid pt-t Iu0j >~pr~IoI 61u.

150K mating SsvB precursor [Streptococcus gordonij iI 110 j9.90E-39 107 I1.70E-38

I

PAc protein precursor (AA -38 to 1527) [Streptococcus T- r EF062-2 jgni IPID d 10 150 surface protein antigen precursor [Streptococcus sobrinus) 1 1 5--OOtE-3 6 6.5013-36 1 .2013-35 EF062 21 giJ47 267 cell surface antigen 1111 [Streptococcus mutans] >p~iY15068391S06839

I

EF062-2 bbsj 148453 SpaA~endocarditis im-munodominant antigen [Streptococcus sobrinus, S S 9*

S

S..

S S S

S

S

S.

S

S S

S..

S S S S S 55*** S S S S S S S S S S S. S* S S Table 2. Closest matching sequences between the polypeptides of the presenit invention and sequences in GenBank and Der-went databases.

EF062-2 giJ47 620 antigen 1 /11 [Streptococcus sobrinus] >pir1A603381A60338 surface 2.90E-35 -1109 12. 1OE-34 EF062-2 EF062-2 EF062-2 EF063-2 EF0632 EF063-2 EF063-2 EF063-2 EF063-2 pirlA3S I86lA35j gI I 009-)r giI097 giJ47 049 gi324 giJ2 109266 -gi1150555 gi1l100973 giJ 4 7 24 8 salivary agglutinin receptor precursor Streptococcus I i. 110 3.80E-32 SspA [Streptococcus gordonii] SspA [Streptococcus gordonij asalI gene product (AA 1- 1296) [Enterococcus faecalis) aggregation substance (ASPI) [Enterococcus faecalis] aggregation substance Enterococcus faeciun] aggregation substance [Plasmid pCF 101 >pirIH41662lH4l66 2 150K mating SspB precursor [Streptococcus gordonii] PAc protein precursor (AA -38 to 1527) [Streptococcus mutansi surface protein antigen precursor [Streptococcus sobrinus] cell surface antigen 1111 [Streptococcus mutans] >pirlS068391SO 68 39 SpaAendocarditis inumunodomiflant antigen [Streptococcus sobrinus, antigen I A~l [Streptococcus sobrinus] >pirlA60338lA60 3 38 surface salivary agglutinin receptor precursor Streptococcus 110 3716 4003 5523 6338 3.80E-2 2.0E2 0 0 0 0 eI EF063-2 gnillPIDjdI10 EF063-2 gi147 267 EF063-2 bbsl 148453 EF063-2 giJ47 620 132 5.OOE-36 107 I6.50E-36 132 132 1 .20E-35 2.90E-35 EF063-2 1861A35 I

I

4 4** 0** .4 *4* 6 V 4 V V

V

V V 44 4

V

4*4 *4e V A 4 *4 VA *4 V* V 0 V *VV*4 *g* *6 C V Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF063-21 gil 1100971 EF063-2 gil 110097 5 EF064-2 giJ470 49 EF064-21j giJ433 24 EF064-2 EF064-2 EF064-2 EF064-2 EF064-2 EF064-2 EF064-2 EF064-2 EF064-2 EF064-2 EF064-2 EF068gi121092 66 gil 150555 gill 100973 giJ4 7248 gnllPlDl 1050 7 gi1j47 267 bbs1 148453 giJ47 62 0 pirIA35 186lA35 gill 100971 gil 100975 gi179 03 98 SspA [Streptococcus gordonii] 110 3.80E-32 SspA [Streptococcus gordoniij 110 2.30E-21 asalI gene product (AA 1-1296) [Enterococcus faecalis] 3716 0 aggregation substance (ASP I) [EInterococcus faecalis] 4003 0 aggregation substance [Enterococcus faeciuml 5523 0 aggregation substance [Plasmid pCF 10] >pirlH41662lH41662 6338 0 150K mating____ SspB precursor fStreptococcus gordonii] 110 9.90E-39 PAc protein precursor (AA -38 to 1527) [Streptococcus 107 1-70E-38 mutans] surface protein antigen precursor [Streptococcus sobrinus] 132 5.OOE-36 cell surface antigen 1/11 [Streptococcus rnutans] 107 6.50Eb-36 >pirlS06 8 39 1S 0 68 39 SpaAendocarditis immnunodominant antigen [Streptococcus 132 1.20E-35 sobrinus, antigen 1 /11 [Streptococcus sobrinus] >pirlA60338lA6033 8 132 2.90E-35 surface salivary agglutinin receptor precursor Streptococcus 109 2. 1OE-34 SspA [Streptococcus gordoniil 110 3.80E-32 sp Streptococcus gordonii] 110 2.3013-21 T06D8. I [Caenorhabditis elegans] 137 85E1 6 C C e.g S C 0e 0O4 S 0 S S *0 C 9

C

S *g C C C C C

C..

CCC egO C C C g.e gee *0 e* g Ceeggee *4 CC C C C Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF068-2 gnIjPlDjdIO208 membrane glycoprotein [Equine herpesvirus 1] 210 5.80E3-16 4 EF068:2 gi(2286 2 04 (AFOI 1339) unknown -[Acinetobacter calcoaceticusl 121 -8.40E3-16 EF068-2 giJ330 86 2 membrane glycoprotein [Equine herpesvirus 1I 208 1.IOE-15 EF068-2 gil 1 7 072 47 partial CDS [Caenorhabditis elegans] 131 3.70E-15 EF068-2 gnlPIDIdIO208 membrane glycoprotein [Equine herpesvirus 1] 203 6.20E- 4 EF068-2 giJ21339 2 antifreeze glycoprotein [Notothenia coniiceps] 102 4.601E-13 84201A3 8420 EF068-2 gnljPIDje12 54 64 (AL022022) PORS-family protein [Mycobacterium 145 1.0E- 12 EF068-2 EF069-2 EF069-2 EF069-2 EF069-2 giJ95 1460 gil790 39 8 gnlPIDId 10208 4 g228 6 20 4 giJ33 08 62 FIM-C.1I gene product L enopus Iaevisj -'pu 1Ij I -ii).i mucin

T

?S2~UL.-I

I

I -rA~r'~Q I rr s,-l~Ik p1po~4n.d Ut-1 Ic membrane glycoprotein [Equine herpesvirus I] 210 156.80E.-10

I

(AFO 11339) unknown [Acinetobacter calcoaceticus] membrane glycoprotein [Equine herpesvirus 1] 208 131 t 3.70E-15 EF069-21 EF069-2 gil 1707247 gnllPlDldlO2O8 4 giJ2 13392 partal CDS Jaenorabditi eleals membrane glycoprotein [Equine herpesvirus I1 anti freeze glycoprotein [Notothenia coriiceps] [6.20E-15 I t 102 I

S

I 4.60E-13 102 4.60E- 13 6 0 *00 000 0 000 00 0 0e S 0 0 0 0 0 0 00 S. @0 0 @0 S 000 *00 @00 0 0 0 0 000000 0@ OS S 0 0 @000005 0000000 0@ @0 0 0 0 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

I

EF069-2 gidlPlDlel2546 4 EF069-2 giJ95l 46

O

EF070-2 giJ 7 9 0398 EF070-2 gnlP1D~d10208 EFO7O-2 giJ22 862 04 EF070-2 giJ3309 62 EF07O-2i gi1170 7247 EF070-2 gnljPIDjdl 0208 4 EF070-2 giI2 13392 EF070-2 gnllIle 125464 EF070-2 gil95 1460 EFO7 1-2 gnIlPlDje30 64 28 EF071-21 giJ8537 5 l EF073-21 gi143830 >pirIA3 8420 jA38420 (AL022022) PGRS-family protein [Mycobacteriunm 145 1 .50E-12 tuberculosis] FIM-C.1I gene product [Xenopus lacvis] >pirIA451551A45155 109 2.70E- 12 mucin____ T06D8. 1 [Caenorhabditis elegans] 137 8.50E- 17 membrane glycoprotein [Equine herpesvirus 1] 210 5.80E- 16 (AFOI 1339) unknown [Acinetobacter calcoaceticus] 121 8.40E- 16 membrane glycoprotein [Equine herpesvirus 1] 208 1.1OE- >pirIH368021VGBEXI 1 partial CDS [Caenorhabditis elegans) 131 3.70E, membrane glycoprotein [Equine herpesvirus 1] 203 6.20E- antifreeze glycoprotein [Notothenia coriiceps] 102 4.60E-13 >pirIA3 8420 1A3 8420 (AL022022) PGRS-family protein [Mycobacteriumn 145 1.50E-12 tuberculosis] FIM-C. I gene product [Xenopus laevis] >pir1A451551A45 155 109 2.70E- 12 mucin unnamed protein product [Bacteriophage nlt] >gill 353566 127 2.00E-37 Lysin N-acetylmuramoyl-L-aaninfe aidase Bacteriophage AL5 11] 273 2.6E3 xpaC [Bacillus subtilis) >gnIPID~d1O0505 3 hydrolysis of 173 7.IE1

A

S S S S S S S S.

S

Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank EF074-2 giJ125 6698 chitinase [Serratia marcescens] >gill 25 6698 chitinase [Serratia 618 2.60E-104 EF074-2 giJ17 63 98 5 chitinase A [Vibrio harveyij 526 2.80E-84 EF075-2 giJ143 156 membrane bound protein (Bacillus subtilis] >gnIIPlDjel 184471 593 1.70E-91 EF075-2 pirlD7007OlD7O transcriptional regulator homnolog ywtF Bacillus subtilis 118 1 .90E-59 0 EF075-2 giJ1762 3 27 putative transcriptional regulator [Bacillus subtilis] 148 9.6013-53 EF07 5-2 giJ 1276874 EpsA [Streptococcus thermophilus] 239 2.20E-33 EFO75-2,gnljPlDje289l2 6 unknown [Streptococcus pneumoniae] 150 1.20E-27 EF075-21 gil 48 5275 putative regulatory protein [Streptococcus pnieumoniae] 150 2.50E-27 I CA I ICM2 and Derwent databases.

EF075-2 EF075-2 EF075-2 EF075-2 EF075-2 EF075-2 gil280 4 735 (AF030367) putative regulatory protein [Streptococcus gil2 804 7 47 gnljlDlel 16988 giJ280 4 7 69 gill 147744 gi1790 43 giJ22 672 39 gnlP1DIdl01 8 9 gnlIPlDldlOI 13 pneurnoniae] (AF030369) putative regulatory protein [Streptococcus pneumoiael

I

2.50 127.

14I ~Ucapsular polysaccharide synthesis protein [Streptococcus (AF030373) putative regulatory protein [Streptococcus pneumoniae] FPSR [Enterococcus hirae] PSR [Enterococcus faeciumjk pir4S5l77IS54177 PSRroteinLZ 148 _L.30Ez I 109 102 5.30E-27 2. 1OE-23 4.40E-19 85E19 109 ORF I [Staphylococcus epidermidis] membrane bound protein LytR [Synechocystis sp.] cadmium-transporting ATPase [Synechocystis sp.] 121

I

2.30E-1 13 2.30E- 113 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

*t.

4

C

*4 4 S 4 4* 4 *4 4* 4* 4* Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF078-2 gnlI1PIDIdlOl 19 homologous to sp:PHOR BACSU [Bacillus subtilis] 219 4.20E-44 EF078-2 gi11 5 7 5578 histidine protein kinase [Thermotoga maritirna) 191__ Ic K7 An AAULI EF078-2 EF078-2 EF078-2 EF078-2 EF078-2 EF078-2 EF078-2 EF078-2 EF078-2 EF078-2 EF078-2 EF079-2 EF081-2 EF081-2 EF081-2 EF081-2 ri' 5zW)QQCI histidine kinase rLactococcus lactis cremorisl I 152 1.I1OE-39 giJ21829 92 gn1(P1 Did101 113 4 gil 14 929 6 giJ58 1188 gn1IPID~dl 0108 7 gnhIPlDje266592 12 182996 gn1P1D~d 10 113 gi129 4893 giJ288 420 giJ209 8 71 9 gi4 6 7 806 giJ790 42 9 ,gnljPlDje208 36 5 g1179 043 3 histidine kinase [Lactococcus lactis cremoris] sensory transduction histidine kinase [Synechocystis sp.] phosphate regulatory protein phoR (gtg start codon) [Klebsiella !hoR gene product (AA 1-431) [Escherichia coli] >gi11657 5 96 sensory transduction histidine kinase [Synechocystis sp.] unknow [Mycobacterium tuberculosis histidine kinase [Lactococcus lactis cremoris) 259 3 .90E-38 226 138 232 206 7 .60E-33 1 .60E-32 3 .70E-32 1. 1 E 31 1.309-31 256 1.30E-31 sensory transduction histidine kinase [Synechocystis sp.] hohate regulatory protein phoR (gtg start codon) [Shigella drug sensory protein A [Synechocystis PCC68031I >gnlIPID d 1017420 putative fimbrial -associated protein [Actinomyces naeslundii) penicillin-binding protein [Enterococcus faecalis) low affinity penicillin-binding protein 5 (PBP5) [Enterococcus penic illin-binding protein 5 Enterococcus faecium] low affinity penicillin-binding protein 5 (PBP5S) Enterococcus 225 106 1 .60E-3 1 2.50E-31I 183 8.60E-26 1356 2.lIOE-178 607 1.OOE-78 604 I.IOE-78 604 2.70E-78

S

S S

S

*5 S *5* a S S S S S S S S S S. Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF081-2 giJ790 437 low affinity penicillin-binding protein 5 (PBP5) [Enterococcus 602 5. 1OE-78 EF081-2 -gil79 043 1 low affinity penicillin-binding protein 5 (PBP5)_[Enterococcus 591 2.60E-77 EF081-2 giJ433 42 D-alanyl-D-alanine carboxypeptidase [Enterococcus hirae] 587 9.30E-77 EF081-2 giI4900 0 ID-alanyl-D-alanifle carboxypeptidase [Enterococcus hirael 572 5.20E-74 EF081-2 gnlIPIDldl0079 penicillin-binding protein 2 [Bacillus subtilis] 149 7.40E-2 4 EF08 1-2 gnl(PID1e315088 MecA 1 [Staphylococcus sciun]l Ill 4.40E-19 EF081-2 gnlPIDle28 66 51 MecA protein [Staphylococcus sciuri] 106 2.90E-18 EF081-2 gnljPIDje3l6581 MecA protein [Staphylococcus sciuri] 11I 2.90E- 18 EF081-2 gnljPIDje3 1660 MecA2 protein [Staphylococcus sciuri] 101 3.70E-14 EF081-2 griIPDe31661 3 MecA prtein [Staphylococcus sciuri] >gi 4 6 613 mecA gene 101 3.70E-,'14 EF083-2 giJ49 6 83 lysin [Bacteriophage Tuc2009] 436 6.205'-176 EF083-2 gi153079 8 LysB [Bacteriophage phi-LC3] 421 3.OOE- 175 EF083-2 gi1 16 6 1 8 3 muramidase [Bacteriophage CP-71 186 1.20E-21 EF083-2 gi16 88 muramidase [Bacteriophage CP-91 >pirJQ0438IMUBPC9 188 5.00E-21 EF083-2 gi162 3084 muramidase; muramridase [Bacteriophage LL-H] 193 8.40E-20 EF832 il 6 t 7 muramnidase Bacteriophage CP-1117 -3.40E-19 EF8--: 1. 6 JA,(61I.At5r 175 -dlFI EF083-2 gn1jPIDje22 1272 EF083-2 pirIJQ04371MU

BP_

EF083-2 gil 4 105 02 EF083-2 g793850 lysozyuie Baceniophage r- iiIr~ j >pUA.pv UU61 N-acetylmuramoyl-L-alaline amidase (EC 3.5. 1.28) phage LysA [Bacteriophage inv4j >pirIS38477IS384 77 lytic enzyme IlysA Ilysin [Lactobacillus bacteriophage phi adh] >gnllPlDe 217 3 1 4 a 9.5 50E- 19 187 8.90E- 17~ 117 5.60E- S S CS S S. S S S S S S S S S *5 S 5 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in Genflank and Derwent databases.

EF084-2 gi1229 33 12 (AF008220) YtfP [Bacillus subtilis] >gnljPIDje 185,879 similar 438 1.70E-140 to EF084-2 gi123672 34 (AE000425) hypothetical 43.8 kD protein in rhsB-pit intergenic 167 2.2013-51 EF084-2 gil9 12464 No definition line found fEscherichia coli] 167 6.0013-51 EF084-2 gnljPIDjd10l 12 hypothetical protein [Synechocystis sp.] >pirlS76 6 78IS 76 67 8 151 6.J1OE-42 7 EF084-2 gil 1573954 hypothetical [Haemophilus influenzael >pirlG64 161 IG64 161 142 2.90E-40 EF085-2 gil 1209527 protein histidine kinase [Enterococcus faecalis] 2023 18.0012-279 EF085-2 giJ4670 5 7 phoR; B2168_C3_247 [Mycobacterium leprae] 226 8.80E-23 ___________>pirj5729051S72905 EF085-2 gnlPIDIel 19229 SenX3 [Mycobacterium, bovis BCG] 222 3. 10E322 EF085-2 gnljP1Dje255l52 unknown [Mycobacterium tuberculosis] >gnlPIDle3215 4 6 222 3.I1OE-22 SenlX3____ EF085-2 gill 77 848 5 PcoS homnolog [Escherichia coli] >gil 1786783 (AE0001 62) M480; 1l1 3.8013-16 This__ EF085-2 gi1149296 phosphate regulatory protein phoR (gtg start codon) [Klebsiella 110 1.40E-14 EF085-2 gil5811 88 phoR gene product (AA 143 1) [Escherichia colij >gill 6 5 7 5 96 103 5.30E-14 EF085-2 6-11143331 alkaline phosphatase regulatory protein [Bacillus subtilis] 118 4.9013-13 EF085-2. gil537239 alternate gene name phoM; CG Site No. 395 [Escherichia colij 126 9.50E-13 EF085-2 giJ147 2 5 1 phoM [Escherichia coli] >gil809670 phoM protein (I is 3rd 126 9.5013-13 base in EF085-2 giJ2 182992 histidine kinase [Lactococcus lactis cremoris] 109 O.9E-12 1111' 949 4 4 4 4 9 4 4 4 4. 4* 9* 9 *4 9. 4 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

I

EF086-21 giJ4377 06 lalternative truncated translation product from E.coli 3.00E-54 ~1 jIStreptococcus I EF086-2 jgi143770 5 jhyaluronidase [Streptococcus pneumoniael 221 41.60E5 EF086-2 Igi1595 847 Ihyaluronate lyase [Streptococcus agalactiae] 203 3.30E-44 I 1371A55 1371 1.t n AAV Ae) EF086&2 EF086-22 FEF087-2 EF087-2 EF087-2 EF087-2 EF087-2 EF088-2 EF088-2 EF088-2 gi1705 406 gi(5620 86 gi14377 06 gil437705 giJ59 58 47 gi1705 406 giJ56 2 086 gij43 7706 giJ437705 giJS958 4 7 hyaluronate lyase [Staphylococcus aureus] hyaluronidase [Propoibacterium acnes) alternative truncated translation product frm E.coli 198 6.OOE-27 3.OOE-54 [StreptocoCCUS hyaluronidase [Streptococcus pneurnoniae] 221 1 .60E-53 hyaluronate lyase [Streptococcus agalactiael 203 3.30E- 44 >pirjA55137jA55 137____ hyaluronate lyase [Staphylococcus aureus] 191 3.40E-42 hyaluronidase [Propionibacterium acnes] 198 6.OOE-27 alternative truncated translation productfr E.coli 221 3.OOE-54 [S treptococcus hyaluronidase [Streptococcus pneumnoniae] hyaluronate lyase [Streptococcus agalactiael 221 203 1.60E-53 1.30E-44 EF088-21 giJ705 4 0 6 hyaluronate lyase Staphylococcus aureus] 1 91 3.40E-42 EF088-21 gij562 086 hyaluronidase [Prop ion ibacteriurn acnes] 198 6.OOE-27 EF091-2 gi5 6 l iia opatwtrsrs roteins; ORF2 [Bacillus subtilis] 198 5.50E-21 EF091- 1 i235 3333 j(AF 16513) Ce-LEA [Caenorhabditis elegans] 189 2.40E-17 9 9 9 S p a 9~ *9 St 9 a. 9 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF091-2 gnt1PIDje353216 seed maturation protein homolog [Arabidopsis thaliana] 146 3.6013-11I EF091-2 gill 161171 late embryogenesis abundant protein [Picea glauca] 132 5.70E-l II EF091-2 pirlS049091SO 49 embryonic protein DC8 (clone 8/10) carrot 127 6.5OE-l II EF092-2 gJ1268989 8 (AE000792) PTS system, cellobiose-specific ti13 component 145 4.OOE-27 ~~(celA) EF092-2 gn1lPlDjdl0204 B. subtilis, cellobiose phosphotransferase system, celA; 116 1.40E-26 8 EF096-2 gJ11 4 7 329 transport protein [Escherichia coli] >gnljPlDjd 10 15409 532 2.I1OE-91 EF096-2 gil 1573475 spermidine/putrescine-binding periplasmic protein precursor 527 1.I1OE-79 (potD) EF096-2 gi1157 480 3 spermidine/putrescine-bindiflg periplasmic protein precursor 468 1.6013-75 EF096- il 10142681 1Lpp38 [Pasteurella haernolytica) 446 4.40E,72 EF096-2 gnIjPlD~d10152 Putrescine transport protein PotF [Escherichia coli] 216 1.5013-54 6 EF096-2 gi1l 4 73 34 periplasmic putrescine binding protein [Escherichia coli] 216 2.I1OE-53 EF096-2 giJ2688 565 (AE001 165) spermidine/putrescine ABC transporter, 240 2.OOE-48 EF096-2 gi1881 733 PotD [Salmnonella typhimuriumn] 253 2.7012-28 EF096-2 gnIjPIDldlOl92 spermidine/putrescine-binding periplasmic protein 243 4.20E-26 6 EF096-2 gnlPlDle]5254 3 potE gene product [Clostridium perfringcns] 204 3.30E-21 EF097-2 gi1622 99 1 mannitol -transport protein [Bacillus stearothermophilus] 547 4.90E-93 EF097-2 gi14203 4 mannitol permease [Escherichia coli] >gi4 66737 mannitol- 535 5.50E-85 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF097-2 giJ633 6 5O enzyme 11(mannitol) [Staphylococcus camosusl 516 2.I1OE-82 ~~>pirIS68 1931522385 EF097-2 giJ8 8246 2 protein-N(pi)-phosphohistidifle-ugar phosphotransferase 509 3.OOE-76 [Escherichia EF097-2 giI3 12763 protein-N(pi)-phoSphohisti dinle-sugar phosphotransferase 357 7.50E-70 EF097-2 gnIlPIDIdlO09 6 homologue of mannitol transport protein of B. 492 3. 1 OEB-62 6 EF097-2 gnlIPIDjd1079 mannitol-specific phophotransferaSe enzyme 11 [Bacillus 484 5.20E-61 2 1 EF097-2 gi11 6 7 3 855 (AEOOOO2O) Mycoplasma pneumoniae, PTS system mannitol- 232 3.50E-\59 specific EF097-2 gn]IPIDIdlOO 6 5 phosphotransferase enzymell, niannitol-specific [Mycoplasma 158 8.20E- 18 1 EF097-2 pirIS77757IS777 phosphotransferase system enzyme 11 (EC 2.7.1.69), 103 2.OOE- 13 EF 100-2 giJ20 5 8546 ComYC [IStreptococcus gordonii] 193 7.30E-27 EF 100-2 gi1205 85 46 ComYC Streptococcus gordonil 193 -7.30E-27 EFI100-2 giJ142708 comnG3 gene product [Bacillus subtilis] >gnljP1Dje1 185739 150 2.90E-22 comGC EF 100-2 gil42708 comG3 gene product [Bacillus subtilis] >gnljPlDje1 185739 150 2.90E-22 comGC___ EFI100-2 gi114 843 7' secretory component [Erwinia chrysanthemui] 134 4.40E- I>pirE 702 IIE4702 D ectic____

S

*5* a S. S

S

S S S S a S. 5 5 a a a. a a S S *5 S a Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank EFI100-2 giJ148 43 7 secretory component [Erwinia chrysanthemi] 134 4.40E- >pirIE4702 1 JE47021I EFJOO-2 giJ6062 62 ORE-o 145 [Escherichia coli] >gil69370 6 HopG [Escherichia 136 9.1013-13 EF 100-2 gi16062 62 ORF-o 145 [Escherichiacoli] >gil6937 0 6 HopG [Eschericbia 136 9.IOE-.13 coli] EFl100-2 giJ388 28 ExeG gene product [Aeromonas hydrophila] 132 3.50E-12 >pirlS229 101149905 protein EF100-2 giJ388 28 ExeG gene product [Aeromonas hydrophila] 132 3.50E-12 >pr S229 101149905_protein____ EFIOO-2 2gnlIlDjel 17259 etpG [Escherichia coli] 131 5.1013-12.

and Derwent databases.

EF 100-2 EFIOO-2 gil 42189

IL

Wietpu13 I 9.90Ec-ac12 ~1 outG gene product [Erwinia carotovora] >pirIS328 6 l1S 32 86 1 outG EFIOO-2t giJ4218 9 outG gene product [Erwinia carotovora] >pirJS3 2 8 6

IIS

32 8 6 1 outG putative [Vibrio cholerae] 130 9.90E- 12 128 9l.90E- 12 EF 10-2~ EF 100-2 EF 101-2 EFIOI-2 EFI11-2 EF 110-2 giJ609 6 8 gi160962 8 gn1lP1DId1 0257 3 IgglIPIDle32 1943 gnilPIDle1 18502 12 6013- 11 putative [ibrio cholerae] bacG [Enterococcus faecalis] hypothetical protein [Enterococcus faecalis] >gn1PlDje32 1943 similar to hypothetical proteins from B. subtilis [Bacillus Staphylococcal serine proteinase homologue [Enterococcus fahecalis] 106 31.60E-17 105 113 3.60E- 17 1.80E-16 1462 2.30E-195 1462 2.3013- 195 S 4 4 4*4 4 9 *4*4 9* S 9~ p

S

S p 9 4 9 *4 44* S. 54 4 p *4 S S S Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank EFi 110-2 gnlIDIdlO0lO glutamic acid specific protease prepropeptide [Staphylococcus 106 3.7013-14 8 EF 10-2 gil46687_ preproenzyme (AA -68 to 268) [Staphylococcus, aureus] 106 6.70E-14 EFI 11-2 giJ6060lS ORF o783 [Escherichia coli] >gi11789 46 2 (AE000390) 477 8. 1OE-80 _________hypothetical_88.3 1_ 1 1 An I -7z] and Derwent databases.

EF 121-2 EF 121-2 EF 12 1-2 EF 121-2 EF 121-2 EF 121-2 EF 121-2 EF 121-2 gi12 626 826 giJ4845 3 giJ75 7 84 2 g11177 3 162 giJ4795O gi17 4 7 9 1 3 giJ627 72 J..3VLL n.K 1 tI* 1ki' 4L'Slfll. eI I i ilrt M, VW a" m f

I

(AE000532) 2'3cci-uloie2-popoiseae(pB 413i Z.OUt- 5'-nucleotidase [Vibrio parahaemolyticus) >gnljPIDjd 100 12 18 UDP-sugar hydrolase [Ecricha olii....

7 39 1,.60FE-44 UDPsu r hdroaseprecursor [Escherip~acolil >gill786~Z. 239

I

precursor polypeptide (AA -25 to 525) [Salmonella typhimurium] 2',3 '-cyclic-nucleotide 2'-phosphodiesterase [Yersiia, 5'-nucleotidase [Discopyge ominata] >pir15 195641Sl1 9 5 64 5'nucleotidase 2' 3' cyclic-nucleotide 2'-pbosphodiesterase (cpdB) 2',T-cyclic-nucleotide 2'-phosphodiesterase [Escherichia colil 1 .60E-44 2I IOE-41 I I 4 .70E-3 6 4.70E-36 EF121-21 gi15 7357 3 137 5.80E-35 114 8.90E-34 110 E;I OE-31 EF12 1-: EF 121-: EF 121-: 2 giJ537 054 bbsj 1359 15 5l..nucleotidaseglycosylphosphatidylinositol-anclhored protein 128 7-70E-29 {EC 5'-nucleotidase [Boophilus microplus] 104 1.60E-28 21 gil 17-3 74,43 1 1.315 0. 1 ULL-/-O EF 12 EF 12 1-I v1Ii1J21 5)flU1ttuLIUorvetyie- 1-1) -I I- p I ceuaeDrcso I1* 2 9Q( 4 9 7 83 jecto-5'-nucleotidase [Mus musculus] >pirli C200 I IJC200 1 1-50 1. 1 VrI-1- 9 a 9 a a 9* .9 *9 9 a 9* a a *9 a 9 9 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EFl121-2 gi1 23897 5'-nucleotidase [H-omosapiens] >pirIS I 10321S1 1032 133 1.60E-27 ___________nucleotidase EF122-2 giJ262 68 26 YfkN [Bacillus subtilis] >gnIPIDiell182774 similar to 143 1.30E-96 EF122-2. g1231 3 187 (AE000532) 2',3'-cyclic-nucleotide 2'-phosphodiesterase (cp B) 413 2.60E-82 EF122-2 gi14 8453 5'-nucleotidase [Vibrio parahaemolyticus] >gnlPDIdlO01218 279 8.50E-47 EF 122-2 giJ757 8 4 2 UDP-sugar hydrolase [Escherichia, colij 239 1.60E-44 EF122-2 giII77 3 1 6 2 UDP-sugar hydrolase precursor_[Escherichia coli] >gil 17 86 687 239 1.60E-44 EF 122-2 giJ4795O precursor polypeptide (AA -25 to 525) [Salmonella 229 2. 1OE-41 typhimurium] EF 122-2 giJ7479 13 2',3'-cyclic-nucleotide- 2'-phosphodiesterase [Yersinia, 115 4.70E-36 EF 122-2 giJ6 2 7 7 2 5'-nucleotidase [Discopyge ommata] >pir1l95 6 4 I1 9 5 64 137 5.80E-35 nucleotidase EF 122-2 giJIS 73 5 73 2',3'-cyclic-nucleo tide 2'-phosphodiesterase (cpdB) 114 8.90E-34 Haemophilus EF 122-2 giJ53705 4 2',3'-cyclic-nucleotide 2'-phosphodiesterase [Escherichia coli] 110 1. 1 OE-31I EF 122-2 bbsl 135915 5'-nucl eotidase= glyco sylphosphati dylinosi to Iancho red protein 128 7.70E-29 EC EF 122-_2 gi117 3 7 443 5'-nucleotidase [Boophilus microplus] 104 -1.*60E-28 EF 122-2 EF 122-2 EF122-2 gi120 255 1 giJ3497 83 giJ23 897 5'-nucleotidase precursor jz- J) tnatu norve -ua ecto-5'nuceotidase[M~us uculus >irJC200JC 2 001 1361

I

.LIOE-27 1 .6013-27 5'-nucleotidase [Homo sapiens] >pirIS I 10321S1 11032 5'nucleotidase (EC I ~,iU EF12 "-9 1I A~i$J~ P54 protein I7Enterococcus faecum >irlS55421S05542 b J U YAUn- /Y I a

I

9 .9 9*9 9

S

9 S

S

S 9. 9**

S

*5 5 9 9 S *9 55 S S 5*9 S* S S Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank ___________hypothetical EF 129-2 guS 12521 usp 45 gene product [Lactococcus lactis] >pirlJNOO97IJN0097 374 1.3013-42 secreted EF 129-2 gi114952 5 secreted protein [Lactococcus lactis] 371 3.60E-42 EF129-2 gnhI]PlDle3l3O22 hypothetical protein [Bacillus subtilis] >gn1P1De 118616 8 317 2.30E-33 EF130-2 giJ 4 8833 9 aipha-amylase [unidentified cloning vector] 621 6.70E-81 EF130-2 gi148833 6 ORF [unidentified cloning vector] 242 8.OOE-27 EF130-2 bbsl 112518 aipha-arnylase {N-tenninal region Artificial sequence, Peptide 237 4.80E-26 EF130-2 gn1,IPIDle28914 4 YwPE [Bacillus subtilis] >gnlIlDjel 1184540 ywpE [Bacillus 129 5.404-l 1 EF131-2 gnljPIDje 11 8 5 2 8 penicillin-binding protein [Bacillus subtilis] 277 7.40E-43 EF131-2 giJ488 33 0 alpha-amylase [unidentified cloninvetr 280 1.30E-731 EF 13 1-2 gi15092 49 No definition line found [Lactobacillus plantarum] 274 11 1.3 EFI131-2 gn]IPID~dl0249 (AB009635) Fmt [Staphylococcus aureus] 170 5.60E-20 EF 131-2 guS 15050 DD-peptidase precursor [Streptomyces'lividans] 131 2.30E- 14 ____________>piTIS48220IS48220 EF13 1-2 giII53448 serine DD-p ptidase [Streptomyces lividans] 131 1.20E- 12 EF132-2 giIl 53 826 adhesin B [Streptococcus sanguis] >pirlA435831A4 35 83 adhesin 1257 2730E- 166

B

EF 132-2, gil 1184932 ScbA [Streptococcus crista] 1248 3.70E- 165 I 14! J.jur,*10z and Derwent databases.

EF132-2 EF 132-2 ~EF 132-2 giJ3 10633 gil393269 gijl.5750 3 0 oAhjoin r~tr~t~-nccii~ Qnrdoniil 1 /14 3. 1LF_ iO.U st. T Von I 1204 .163 adhesion protein [Streptococcus pnunme 1204 3.40Et-t I 1220 2AUL-1~l surface adhesin A prcro Srpoccu nuoie 122

I

5 5 S S S S S S S S S 55 S SS S S S *5 55 5 S Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF13272 EF13 2-2 EF 132-2 EF 132.2 EF 132-2 EF 132-2 EF 132-2 EF 132-2 EF132-2 EF 132-2 Query EF003-2 EF003-2 EF006-2 EF006-2 EF008-2 EF008-2 FOO8-2 EF008-2 EF009-2 gill153 83 4 gill 117994 gi14930l 7 gnl1PlDle2555 2 9 gi112 45464 gil 1573330 g117550 7 5 gnljPlDel 118595 gi1177 79 33 gil790 546 adhesin specific for salivary pellicle of dental surfaces 1203 4.80E-159 surface antigen A variant precursor [Streptococcus pneumoniael 1191 2.OOE-1 57 endocarditis specific antigen [Enterococcus faecalisl 931 3.70E-122 lipoprotein [Staphylococcus epidermiidis] 453 3 .20E-92 YfeA [Yersinia pestis] >gil 2 454 64 YfeA [Yersinia pestis] 364 3.60E-64 adhesin B precursor (fim.A) [Haemophilus influenzae] 349 3.50E- 63 per-iplasmic-bindiing protein [Synechocystis sp 326 6. 80E-62 >gnllPIDld 10 1 6 5 2 Mn [similar to ABC transporter (membrane protein) [Bacillus 174 3.10E-32 ~TroA [Treponema pallidum) 171 3.40E-32 Trm I [Treponema palliduml 171 5.10E-32 Derwent Gene Description

IBLAST

Derwven t Access. No.

W20909 W20166 W20909 W20 166 R37495 W26367 R79722 W22134 W20909 pylori outer membrane protein 14gel 0705orf5.

Helicobacter pyloriouter membrane protein, 1 6225006.aa.

H. pylori outer membrane protein 14gelO0705orfS.

Helicobacter pyloni outer membrane protein, 1 6225006.aa.

Pneuniococcal fimbrial protein A.

Staphylococcus aureus saliva binding protein.

ROM precursor

TROMPI.

Treponema pallidum rare outer membrane protein (TROMP-I).

H. pylori outer membrane protein 14ge1705orf5.

268 241 283 266 967 467 181

BLAST

P-Value 4.20E-39 3 .OOE-27 1 .20E-48 1IAOE-30 1 .20E-1 27 7.50E-100 8.00E-36 8.OOE-36 1 .40E-53 9 9 S 0 0 4 5 S S 0t S S Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in Genflank and Derwent databases.

EF009-2 W20 166 Helicobacter pylori outer membrane protein, 16225006.aa. 278 2.5012-32 EFO 12-2 R48035 Hyaluronic acid synthase of Streptococcus equisimilis. 227 3.20E-69 EF014-2 W 14070 S.thermophilus exopolysaccharide biosynthesis protein EpsR. 103 5.90E- 19 EF014-2 W22 169 S.thermophilus exopolysaccharide synthesis operon epsA gene 103 7.30E-18 EF016-2 W 15799 Adherence factor 104R of Lactobacillus fennentum. 157 9.60E-22 EF016-2 W 15793 Adherence factor consensus sequence. 103 1.00 E- I I EF017-2 R48035 H-yaluronic acid synthase of Streptococcus equisimilis. 241 8.90E-71 EF021-2 R31013 P39-alpha. 141 1.60E-19 EF021-2 R33280 P39-beta. 134 7.OOE- 14 EF022-2 R48035 Hyaluronic acid synthase, of Streptococcus eguisimilis. 324 2.2013-65 EF023-2 R48035 Hyaluronic acid synthase of Streptococcus equisimilis. 155 9.90E 33 EF023-2 R70152 Streptococcus; pneunioniae strain SPRU98 PIpA. 125 5.90E- 17 EF027-2 R48035 H-yaluronic acid synthase of Streptococcus equisimilis. 233 2.20E-34 EF028-2 W17830 Thermophilic alkaline phosphatase. 202 7.70E-59 EF028-2 WI 11568 E.coli alkaline phosphatase mutant D I53H/Q329A. 182 7.90E-56 EF028-2 W 11570 Ecoli alkaline phosphatase mutant D I53H/K328H/Q329A. 182 7.90E-56 EF028-2 W26300 E.coli alkaline phosphatase mutant 182 1.l1OE-55 ________D153H/K3281Q329A1D330H.

EF028-2 WI 11565 -E.coli alkaline phosphatase mutant D IS3H/K328141D330A. 182 3.I1OE-55 EF028-2 WI 11557 E.coli alkaline phosphatase mutant D I 3HID33ON. 182 4.3013-5 EF028-2 WI 1561 E.coli alkaline phosphatase mutant D153H/D330A. 182 4.30E3-55 EF028-21 WI 1555 E.coli alkaline phosphatase mutant D153H/K328E/D330N. 182 4.7013-55 e a a a. a Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF028-2 WI 1566 E.coli alkaline phosphatase mutant DlI 531-11K3281D330L. 182 1 .20E-54 EF028-2 Wi 1569 E.coli alkaline phosphatase mutant K328H1Q329A. 180 1 .7011-54 [EF028-2 WI 1562 E.coli alkaline phosphatase mutant D I53H1D330L. 182 1.7013-54 EF028-21 R26980 Fv(FRP5)-phoA recombinant antibody. 174 1.90E-54 EF028-21 Wi 1567 E.coli alkaline phosphatase mutant Q329A. 179 2.30E-54 EF028-2 Wi11558 E.coli alkaline phosphatase mutant K328H1D330N. 176 6.40E-54 EF028-2 WI 11563 IE.coli alkaline phosphatase mutant K328H1D330A. 176 6.40E-54 EF029-2 R10044 Plasmid pOW360 encoded Human Growth Hormone (HGH) 320 13.50E-40 nuclease A EF029-2 R10041 Plasmid pOW350 nuclease A product. 320 4.3013-40 EF029-2 R73997 Staphylococcus aureus (Foggi) nuclease signal and mature 320 5.60E-40 sequences.___ EF029-2 R10043 Plasmid pOW360 encoding Human Growth Hormone (HGH) 320 2.90E-38 nuclease_____ EF030-2 R48035 Hyaluronic acid synthase of Streptococcus eguisimilis. 277 6.I1OE-47 EFO4O--2 R59077 2-5A-dependent KNA-ase. 105 1.90E- 18 EF040-2 W12703 Mouse 2-5A-dcpendent RNase. 105 1.90E- 18 EF040-2 R82661 Partial murine 2-A-dependent RNase. 105 1.9013-18 EF041-2 R48035 1-lyaluronic acid synthase of Streptococcus euisimilis. 225 6.30E-26 -IQr Q nnA:;--A EF054-2 EF054-2 R26042 R85782 OCIDII f Ir 232 OE-24- Group B Streptococcal mutant beta antigen without IgA binding domain.

GopBStreptococcal wild-type beta antigen.

232 3.30E_24

EFC

-1 1? R 8 1 .1 J 4 *4* 4 U* 9 4 *44 4.4 4 4 4 4 *4 44 4 ~4S** 4. 4* 9 4 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in Genl~ank and Derwent databases.

EF054-2 P91941 Sequence of preprospasmolysin. 204 3.lIOE-19 EF054-2 W32519 Collagen-like polypeptide SEQ ID NO:2. 180 7.50E- 18 EF054-2 Wi 12324 Silver halide emulsion protein monomeric repeat unit 180 7.50E- 18 EF054-21 W32522 Collagen-like polypeptide SEQ ID NO:5. 192 1.60E- 17 EF054-2 W 12327 Silver halide emulsion protein monomeric repeat unit 192 1.60E-17 EF054-2 W32520 Collagen-like polypeptide SEQ ID NO:3. 189 2.40E- 17 EF054-2 W32532 Collagen-like polypeptide, SEQ ID NO: 15. 189 2.40E-17 EF054-2 W 12325 Silver halide emulsion protein monomeric repeat unit 189 2.40E-17 EF054-2 W12337 Silver halide emulsion protein monomeric repeat unit 15. 189 2.40E- 17 EF054-2 W 12341 Silver halide emulsion FLAG(RTM)-tagged protein 189 2.60E- 17 EF054-2 W02098 S. mutans antigen 1/11. 161 5.40Ei15 EF054-2 W02096 S. mutans antigen 1/11 fragment (aa803-1 114). 161 1.90E-13 EF059-2 R.26042 P. yoelii SSP2 antigen. 344 1.90E-39 EF059-2 R85782 Group B Streptococcal mutant beta antigen without IgA binding 232 1. 1 OE-26 domain._____ EF059-2 R85781 Group B Streptococcal wild-type beta antigen. 232 l.70E-26 EF059-2 P91941 Sequence of preprospasmolysin. 200 .1.50E-18 EF059-2 P60570 Sequence of the Falciparuin Interspersed Repeat Antigen 186 4.60E- 18 EF059-2 W02096 S. mutans antigen 1/Il fragment (aa803-1 114). 167 8.20E-16 EF059-2 W02098 S. mutans antigen 1/11. 167 4.90E- EF059-2 R79625 Endocarditis specific antigen region. 147 4.40E- 12 EF059-21 R26049 MSF precursor. 143 1.30E-lIl JEF059-21 R28 150 ISugar beet chitinase 1. 148 l .70E-1I1 400 a a

S

*4* 0 0 a.

04 0 a a a *0* 0 040 *5 9 0 0* a Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF059-2 R26842 Protease from S. Aureus ATCC 12600. 147 2.10E-1 I EF059-2 R79643 Inununodominant antigen of Streptococcus sobrinus. 151 2.IOE-1 I EF059-2 W07539 Collagen like protein 146 3.00E-1I1 EF06 1-2 R26042 P. y~elii SSP2 antigen. 241 1.70E-25_ EFO61:-2 P60570 Sequence of the Falciparum, Interspersed Repeat Antigen 199 1.60E-18 EF061-2 R85782 Group B Streptococcal mutant beta antigen without IgA binding 153 2.40E-14 Sdomain, EF061-2 R85781 Group B Streptococcal wild-type beta antigen. 153 3.60E- 14 EF06 1-2 P91941 Sequence of preprospasmolysin. 163 9.7013-14 EF061-2 P83194 Sequence of a bioadhesive precursor protein encoded by cDNA 156 7.90E- 13 Sclone EF06 1-2 R28 150 Sugar beet chitinase 1. 156 9. 10OF- 3 EF061-2 W02096 S. mutans antigen 1/11 fragment_(aa803-1 114). 148 1.20E-12_ EF061-2 P82971 Bioadhesive precursor protein from cDNA 52. 148 9.70E- 12 EF061-2 W02098 S. mutans antigen 1/1. 148 1.50E-1-1 EF062-2 W02098 S. mutans antigen 1/11. 107 1.20E-36 EF062-2 R79643 Imniunodominant antigen of Streptococcus sobrinus. 132 3.OOE-36_ EF063-2 W02098 S. mutans antigen 1/1l. 107 1.20E-36.

EF063-2 R79643 Immunodominant antigen of Streptococcus sobrinus. 132 3.OOE-36 EF064-21 W02098 S. mutans antigen 1/H1. _107 1.20E-36 EF064-2 R79643 Inmunodominant antigen of Streptococcus sobrinus. 132 3.0013-36 EF071-2 R85294 Phage R1I-t LytR lysin. 127 3.70E-38 EF071-2 R91515 IListeria phage lysin PLY51 1. 273 4.70E-37 gC* CC. C C A

C

*dg

SOC

go

CC

eeg g C C CO C Ce C C C SC C C g CS C SC g

C..

o* Ce Co e 0 0^0 go CC S Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank EF075-2 W 14070 S.thermophilus exopolysaccharide biosynthesis protein EpsR. 239 4.20E-36 EF075-2 W22 169 S.thermophilus exopolysaccharide synthesis operon epsA gene 239 4.OOE-34 EF077-2_ R97280 Helicobac ter-specific ATPase 439. 258 4.10OE-74 EF077-2 R48036 Mycobacteriurn BCG immunogen. 192 2.20E-67 EF077-2 W06712 Helicobacter-specific ATPase 948 (ORF-4). 220 2.50E-67 EF077-2 R70419 Rat homologue of human Wilson disease gene ATP7B. 186 9.80E-54 EF077-2 R72343 Wilson disease protein ATP7B3. 176 6.70E-40 EF077-2 R06376 Product of the ssc 1 gene. 166 3.I1OE-28 EF077-2 R75396 Flea sodium pump alpha subunit. 146 2.40E-25 EF077-2 W20891 pylori transporter protein, l14ce22l9orfl. 15,6 8.60E-14] EF078-2 R56667 Bacteroides fagilis RprX regulatory response protein. 148 8.0-1 EF078-2 R74630 Tomato TGETRI ethylene response protein. 130 7.80E- 13 EF078-2 R69849 Ethylene response (ETR) gene product. 128 1 .70E-1 1 EF078-2 R69850 Ethylene response (ETR) mutant protein etrl-1. 128 1.70E-l I EF078-2 R69851 Ethylene response (ETR) mutant protein etrl-2. 128 1.70E-1lI EF078-2 R69852 Ethylene response (ETR) mutant protein eirl1-3. 128 1 .70E-1 I EF078-2 R69853 Ethylene response (ETR) mutant protein etrl-4. 128 1. 70E- I I EF078-2 R24296 Regulatory protein VanS involved in glycopeptide resistance. 142 2.70E-l I1 EFOSI-2 R27253 Penicillin binding protein PBP2A-epi. 101 4.70E- 16 EF081-21 R27256 Penicillin binding protein PBP2A-27R. 101 6.OOE- EF081-21 R27257 Penicillin binding protein derivative N 1. '101 6.20E- EF081-21 R27258 Penicillin binding protein derivative 101 ,6.20E- and Derwent databases.

S. S

S..

06 S S 6 esS S 5 S S 0 5 S 0 0 Se S. 6 S

S

*5@ 0Se0O@ S 5 0 S 055655 5S @0 0 0 *5 SeeS.

066eOSO 0@ Se 5 5 S Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

E F081-2 R27259 Penicillin binding protein derivative 101 6.20E-15 EF081-2 R27260 Penicillin binding protein derivative 101 6.20E- EF081-2 R27261 Penicillin binding protein derivative 101 6.20E-l15 EF081-2 R.27263 Penicillin binding protein derivative 101 6.20E- EF081-2 .R27264 Penicillin binding protein derivative 101 6.20E-15_ EF081-2 R27262 Penicillin binding protein derivative 101 6.5013-15 EF081-21 R30845 Sequence encoded by the mec A gene. 101 6.90E- EF081-2 R27255 Penicillin binding protein PBP2A-27R. 101 6.90 E- EF081-2 R31216 Penicillin binding protein PBP2A-27R. 101 7.OOE-15 EFI 10-2 R91042 V8 mature protease (aa 1-213). 106 6.60E-l16 EF 10-2 R91043 V8 mature protease (aa 1-214). 106 16 EFl 110-2 R91044 V8 mature protease (aa 1-2 15). 106 7.80E- 16 EFi 10-2 R26842 Protease from S. Aureus ATCCl12600. 106 6.70 E- EF 10-2 R29644 Protease from S. Aureus. 106 1.20E-l14 EFI110-2 W22218 Protein encoded by pV8RPT(-) construct. 106 7.6013-14 EF 110-2 R91033 Beta-galactosidase-V8 protease fusion protein. 106 7.60E- 14 F1 10-2 R91034 Beta-galactosidase-V8 protease fusion protein. 106 l1.70E- 13 EF 110-2 W22219 Protein encoded by pV8D construct. 106 7.60E- 13 EFI 10-2 R91035 Recombinant V8 protease V8D fusion protein. 106 7.60E- 13 EF 10-2 W22220 Protein encoded by pV8F construct. 106 -7.90E- 13 EF 129-2 R14530 Usp 5 protein. 374 2.40E-43 EF129-2, R14 150 MSP encoded by pUCRS (DSM 5803). .372 4.70E-43 EF 131-21 R37495 Pneumococcal fimbrial protein A. 1185 6.80E-163 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

I I I E131-2 EFF131-21 W26367 R79722 Staphylococcus aueus saliva bindin rotein. 418 ROM precursor TROMP 1.

Treronema pallidurn rare outer membrane protein TROMP-i) 171 171 19.OOE-31 W22134 EF 131-2 111 288 TABLE 3. Conservative Amino Acid Substitutions.

Aromatic Hydrophobic Polar Basic Acidic Small Phenylaanine Tryptophan Tyrosine Leucine Isoleucine Valine Glutamine Asparagine Arginine Lysine Histidine Aspartic Acid Glutamic Acid Alanine Serine Threonine Methionine Glycine 9* 289 Table 4. Residues Comprising Antigenic Epitope-Bearing Portion.

EFOO 1-2 from about Asp-i15O to about Lys- 152, from about Ser-256 to about.Tyr- 259, from about Lys-360 to about Lys-363, from about Asn-406 to about EF002-2 from about Asp-80 to about Asp-83, from about Asp-281 to about Gly- 283.

EF003-2 from about Asn-263 to about Gly-266.

EF004-2 from about Asn-23 to about Asn-26, from about Lys-83 to about Ser-87, from about Tyr- 154 to aboiit-Asp- 159.

EF005-2 from about Lys-249 to about Glu-252.

EF006-2 from about Gly-23 to about Asp-28.

EF008-2 from about Thr-92 to about Gly-94, from about Pro- 161 to about Asp- 165, from about Gly-287 to about Thr-289.

EFO 1 0-2 from about Pro- 129 to about Asn- 13 1.

EFO 12-2 from about Asp-77 to about Asp-79, from about Asp-94 to about Lys-98, from about Asp-256 to about Thr-258,- from about Glu-461 to about Asn- 468.

EF013-2 from about Thr-30 to about Asp-32, from about Glu-73 to about from about Gln-1 64 to about Asn- 166, from about Lys- 193 to about Gly- 195.

EFOI14-2 from about Ser-203 to about Asp-206, from about Gin-3 14 to about Gly- 316 EFO 15-2 from about Pro-66 to about Gly-69.

EF016-2 from about Lys-236 to about Asn-239.

EF017-2 from about Ser-90 to about Gly-93, from about Thr-1 97 to about Lys- 199, from about Lys-230 to about Asn-233, from about Ser-428 to about GIY-431.

EF018-2 from about Lys-159 to about Tyr-161, from about Asn-I 65 to about Ser- 167, from about Asn-250 to about Arg-256, from about Asn-392 to about from about Lys-416 to about Tyr-418, from about Asn-428 to a 290 Table 4. Residues Coinprising Antigenic Epitope-Bearing Portion.

Arg-430.

EFOI19-2 from about Arg-209 to about Ser-21 1, from about Lys-287 to about Ser- 290.

EF020-2 from about Lys-57 to about Asn-62.

EF021 -2 from about Ser-33 to about Gly-35, from about Glu-77 to about Gly-8 1, from about Asp- 139 to about Lys- 4 1, fromn about Glu-255 to about Ser- 258, from about Gln-27 I to about Tyr-277.

EF023-2 from about Lys-232 to about Asp-234, from about Arg-304 to about Gly- 306, from about Thr-453 to about Arg-456, from about Ser-478 to about Thr-480.

EF025-2 from about Arg- 18' to about Asp- 18 EF026-2 from about Ser-25 to about Asp-30, from about Asp-90 to about Asp-94, about Gln- 107 to about Asn-1I EF027-2 from about Gln-72 to about Lys-74, from about Lys-229 to about Asp- 231._ EF028-2 from about Asp-I 86 to about Gln- 188.

EF029-2 from about Asp-Il 18 to about Lys- 122, from about Asp- 124 to about Tyr-1 26.

EF031-2 from about Glu-30 to about Gly-33.

EF034-2 from about Glu-25 to about Gly-27, from about Glu-75 to about Thr-77.

EF36-2 from about Gln- 177 to about Ser- 179.

EF037-2 from about Ser-25 to about Asp-30, from about Asp-90 to about Asp-94, about Gin-]107 to about Asn- 110.

EF038-2 from about Asn-77 to about Lys-79, from about Tyr-88 to about Asn-92.

EF040-2 from about Lys-] 67 to about Gly-1 72, from about Lys-240 to about 291 Table 4. Residues Coml-prising Antigenic Epitope-Bearing Portion.

EF044-2 from about Arg- 192 to about Gly- 194, from about Asn-200 to about. Asn- 203.

EF045-2 from about Asp-159 to about Asn-161, from about His-172 to about Gly- 174, from about Tyr-261 to about Gly-264, from about Lys-305 to about E5046-2 from about Ser-IS8 to about Gly-23, from about Gln-41 to about Ser-47, from about Thr-76 to about Asp-78.

EF047-2 from about Asn-28 to about Asp-30, from about Asp-273 to about Asn- 277.

EF048-2 from about Asp- 13 8 to about Lys- 14 1, from about Asp- 15 2 to about 1-2 from about Asp-73 to about Gly- 76 EF053-2 from about Ser-79 to about Gly-82.

EF055-2 from about Asp-26 to about Gly-28, from about Gln-67 to about Asp-69, from about Arg-71 to about Gly-74, from about Arg-87 to about Gly-89.

EF056-2 from about Arg-71 to about Gly-74, from about Arg-87 to about Gly-89 EF058-2 from about Lys-129 to about Gly-133, from about Gin-571 to about Tyrfrom about Pro-586 to about Gly-59 1.

EF065-2 from about Ser-236 to about Tyr-239, from about Asp-350 to about Gly- 3 52, from about Lys-415 to about Asn-4 18, from about Arg-446 to about Asp-448, from about Asn-489 to about Lys-491, from about Ser-516 to about Asp-S 18, from about Glu-639 to about Lys-642.

EF066-2 from about Ser-236 to about Tyr-239, from about Asp-350 to about Gly- 352, from about Lys-415 to about Asn-4 18, from about Arg-446 to about Asp-448, from about Asn-489 to about Lys-49 1, from about Ser-5 16 to Asp-S 18, from about Glu-63 9 to about Lys-642.

EF067-2 from about Ser-236 to about Tyr-239, from about Asp-350 to about Gly- 352, from about Lys-41 5 to about Asn-4 18, from about Arg-446 to about Asp-448, from about Asn-489 to about Lys-49 1, from about Ser-5 16 to Asp-518, from about Glu-639 to about Lys-642.

292 Table 4. Residues Comprising Antigenic Epitope-Bearing Portion.

EF073-2 from about Met-98 to about Arg-100, from about Arg-I 10 to about Asp- 112 EF074-2 from about SCT-53 to about Tyr-59, from about Ser-86 to about Gly-88, from about Pro-97 to about Gln-l100, from about Gln-230 to about Gly- 232.

EF076-2 from about Asn-38 to about Tyr-40, from about Asp-48 to about Asn-53, about Lys-79 to about Gly-8 1.

EF077-2 from about Arg-41 I to about Gly-4 13.

EF078-2 from about. Thr-294 to about Gly-296, from about Asp-366 to about GIn- 368, from about Glu-524 to about Gly-526.

EF080-2 from about Glu- 164 to about Gly-1 66, from about Ser-206 to about Tyr- 208, from about Lys-239 to about Gly-243.

EF081-2 from about Asn-7 to about Ser- 11, from about Lys-77 to about from about Lys-1 12 to about Asn-1 14, from about Gly- 162 to about Aspfrom about Mrg-I 81 to about Gly- 183.

EF083-2 from about Gin-38 to about EF084-2 from about Lys-140 to about Asp-142, from about Gly-164 to about Mrg- 166, from about Arg-262 to about Gly-264.

EF085-2 from about Asn-95 to about Asp-97, from about Arg-1 12 to about Asp- 11 4, from about Asp-258 to about Ser-260, from-about Arg-40 1 to about EF086-2 from about Pro-i 12 to about Gly-1 15, from about Ser-222 to about Ser- 224, from about Asn-296 to about Gly-299, from about Thr-346 to about from about Asp-428 to about Ser-432.

EF087-2 from about Pro- 112 to about Gly- 15, from about Ser-222 to about Ser- 224, from about Asn-296 to. about Gly-299, from about Thr-346 to about Lys-348, from about Asp-428 to about Ser-432.

EF088-2 from about Pro-I 112 to about Gly-1 15, from about Ser-222 to about Ser- 224, from about Asn-296 to about Gly-299, from about Thr-346 to about from about Asp-428 to about Ser-432.

293 Table 4. Residues Comprising Antigenic Epitope-Bearing Portion.

EF090-2 from about Arg-2 to about EF09 1-2 from about Gln-40 to about Asp-43.

EF093-2 from about Lys-95 to about Gly-97.

EF094-2 from about Asp-314 to about Asp-3 16.

EF095-2 from about Ser-328 to abou -t Thr-330, from about Asp-359 to about Asp- 363, from about Glu-637 to abbut Gly-6 3 9 from about Asn-744 to about Gly-746.I EF096-2 from about Pro-128 to about Asn-130, ftrm about Ser-193 to about Asp- 196.

EF097-2 from about Val-357 to about Gly-3 59.

EF099-2 from about Glu-44 to about Asp-47, from about Lys-] 54 to about Gly- 156, from about Asn-286 to about Asp-289.

EFIO1-2 from about Lys-40 to about Asp-42, from about Pro-255 to about Asnfrom about Lys-288 to about Gly-29O.

EF102-2 from about Asp-314 to about Asp-3 16.

EFi 03-2 from about Asn-46 to about Gly-48.

EFi 04-2 from about Pro-232 to about Lys-237, fromn about Ala-362 to about Asn- 366, from about Ser-421 to about Gly-423, from about Lys-488 to about Ser-490, from about Asp-S 50 to about Asn-552, from about Pro-637 to about Lys-640, from about Asp-727 to about Gly-729, from about Asn- 751 to about Ser-754, from about Lys-77 I to about Asn-774, from about lle-835 to about Asn-837, from about Pro-851 to about Gly-853.

EFI 05-2 from about Ser-40 to about Gly-43, from about Asn-94 to about Gln-97, from about Gln-220 to about Gly-222, from about Asn-263 to about Gly- 265.

EF106-2 from about Asp- 72 to about Gly-75, from about Thr-274 to about Asp- 277, from about Asn-3 10 to about Arg-3 13.

a.

EF107-2 EF1O7-2 1 from about Thr- 155 to about Asn- 157, from about Thr- 189 to about Asp- 294 Table 4. Residues Comprising Antigenlic Epitope-Beanng Portion.

191, from about Arg-270 to about Gly-272, from about Thr-330 to about Lys-335, from about Asp-365 to about Asp-368, from about Pro-AS to about Asp-453, from about Gly-485 to about Thr-488.

a a EF 108-2 from about Lys- 142 to about Trp- 145, from about Thr- 147 to about Tyr- 150, from about Arg-212 to about Gly-214, from about Ser-248 to about 1, from about Asp-3 84 to about Asp-3 87, from about Pro-481 to about Arg-483, from about Lys-491 to about Gly-494, from about Thr- 619 to about Gly-624, from about Asp-656 to about Asp-659, from about Lys-717 to about Asn-721, from about Ser-822 to about Gly- 8 2 4 from about Tyr- 137 to about Thr-1l 14 1.

EFI 110-2 from about Pro-123 to about Gly-127, from about Thr-223 to about Gly- 225.

EFI 11-2 from about Lys-207 to about Asn-209, from about Asp-245 to about Asn-248, from about Lys-396 to about Asp-398, from about Giu-429 to about Ser432, from about Thr-470 to about His474.

EFI 19-2 from about Asp-90 to about Asn-92, from about Gln- 142 to about Gly- 144.

EFl121-2 from about Asn-1 59 to about Asp- 16 1, from about Asn-35 1 to about Lys-353, from about Pro-658 to about Gly-660, from about Lys-786 to Ser-789.

EF122-2 from about Asn-159 to about Asp-161, from about Asn-351 to about Lys-353, from about Pro-658 to about Gly-660, from about Lys-786 to Ser-789.

EF123-2 from about Asn-331 to about Arg-336, from about Asp-634 to about Gly- 636, from about Glu-780 to about Ser-782, from about Tyr-909 to about Asn-9 11, from about Lys-939 to about Glu-942, from about Asp- 1074 to about Gly-1076, from about Asp-1367 to about Gly-1369, from about Pro-I433 to about Lys-1435, from about Gly-1516 to about Asp-iS 18, from about Lys-16S6 to about Asp-1 660, from about Lys- 1860 to about Gln-1863, from about Ser-1916 to about Gln-1919, from about Pro-i940 about Gly-1942.

EF124-2 from about Asn-331 to about Arg-336, from about Asp-634 to about Gly- 636, from about Glu-780 to about Ser-782, from about Tyr-909 to about Asn-91 11, from about Lys-939 to about Glu-942, from about Asp- 1074 to ab out (3ly-10 7 6 from ab out Asp- 13 67 to about Gly- 13 69, from about Pro-1433 to about Lys-1435, from about Gly-1516 to about Asp-IS 18, 295 Table 4. Residues Comprising Antigenic Epitope-Bearing Portion.

from about Lys-1656 to about Asp-1660, from about Lys-1860 to about Gln-1863, from about SeT-1916 to about Gln-1919, from about Pro-1940 to about Gly- 1942.

EF 125-2 from about Asn-331 to about Arg-336, from about Asp-634 to about Gly- 636, from about Glu-780 to about Ser-782, from about Tyr-909 to about Asn-91 1, from about Lys-939 to about GIL-942, from about Asp-1074 to about Gly-1076, from about Asp-1367 to about Gly-1369, from about Pro-1433 to about Lys-1435, from about Gly-15l6 to about Asp-iS 18, from about Lys- 165 6 to about Asp- 1660, from about Lys- 18 60 to about Gln-1863, from about Ser--191.6.to about Gln-1919, from about Pro-1940 about Gly-1942.

EF 126-2 from about Ser-236 to about Tyr-239, from about Asp-350 to about Gly- 352, from about Lys-415 to about Asn-418, from about Arg-446 to about Asp-448, from about Asn-489 to about Lys-491, from about Ser-516 to Asp-S 18, from about Glu-639 to about Lys-642._ EF 127-2 from about Ser-236 to about Tyr-239, from about Asp-350 to about Gly- 352, from about Lys-4 15'to, about Asn-4 18, from about Arg-446 to about Asp-448, from about Asn-489 to about Lys-491, from about Ser-516 to about Asp-5 18, from about Glu-639 to about Lys-642.

EF128-2 from about Ser-236 to about Tyr-239, from about Asp-350 to about Gly- 352, from about Lys-415 to about Asn-418, from about Arg-446 to about Asp-448, from about Asn-489 to about Lys-491, from about Ser-516 to Asp-S 18, from about Gu-639 to about Lys-642.

EF 129-2 from about Asn-300 to about Gly-302, from about Ser-3 16 to about Glyfrom about Asn-385 to about His-387 EF 131-2 from about Lys-20 I to about Tyr-204, from about Glu-263 to about Ser- 266.

EF132-2 from about Thr-26 to about Ser-28.

a.

a a.

a a a .aa.

a a..

a a. a a a a.

296 INDICATIONS RELATING TO A DEPOSITED MICROORGANISM (PCT Rule 13bis) A. The indications made below relate to the microorganism referred to int the description on page 10 lIine 12 B. IDENTIFICATION OF DEPOSIT Further deposits are identified on an additional sheet Nan., of depositary institution American Type Culture Collection Address of depositary institution (including postal code and country) 10801 University Boulevard Manasas, Virginia 20110a-2209 United Siates of America C. ADDITIONAL INDICATIONS (leave blank ifnot applicabl) This information is continued on an additional sheet E D. DESIGNATED STATES FOR WHICH INDICATIONS ARE MADE (if the indications are not for all designated States) E. SEPARATE FURNISHING OF INDICATIONS (leave blank if(not applicable) The indications listed below will be submitted to the International Bureau later (specify the general nature of the indication. 'A4ccession Number ojDspoasit) For receiving Office use only This sheet v~ eeved with the international application -For International Bureau use only W1 This sheet was received by the International Bureau on: Authorized officer

Claims (29)

1. An isolated polynucleotide comprising the nucleic acid sequence of ORF EF122-1, represented by SEQ ID NO: 453.

2. The isolated polynucleotide of claim 1, wherein said polynucleotide comprises a heterologous polynucleotide sequence.

3. The isolated polynucleotide of claim 2, wherein said heterologous polynucleotide sequence encodes a heterologous polypeptide.

4. A method for making a recombinant vector comprising inserting the isolated polynucleotide of claim 1 into a vector.

5. A nucleic acid sequence complimentary to the polynucleotide of claim 1.

6. A recombinant vector comprising the isolated polynucleotide of claim 1.

7. The recombinant vector of claim 6, wherein said polynucleotide is operably S. associated with a heterologous regulatory sequence that controls gene expression.

8. A recombinant host cell comprising the isolated polynucleotide of claim 1.

9. The recombinant host cell of claim 8, wherein said polynucleotide is operably associated with a heterologous regulatory sequence that controls gene expression. An isolated polynucleotide fragment comprising a nucleic acid sequence which hybridizes under hybridization conditions, comprising hybridization in and 50% formamide at 50-65 0 C and washing in a wash buffer consisting of 0.5XSSC at 50-65°C, to the complementary strand of ORF EF122-1, represented by SEQ ID NO:453. 297

11. The isolated polynucleotide of claim 10, wherein said polynucleotide comprises a heterologous polynucleotide sequence.

12. The isolated polynucleotide of claim 11, wherein said heterologous polynucleotide sequence encodes a heterologous polypeptide.

13. A method for making a recombinant vector comprising inserting the isolated polynucleotide of claim 10 into a vector.

14. A nucleic acid sequence complimentary to the polynucleotide of claim A recombinant vector comprising the isolated polynucleotide of claim

16. The recombinant vector of claim 15, wherein said polynucleotide is operably associated with a heterologous regulatory sequence that controls gene expression.

17. A recombinant host cell comprising the isolated polynucleotide of claim i. 18. The recombinant host cell of claim 17, wherein said polynucleotide is operably associated with a heterologous regulatory sequence that controls gene S. expression.

19. An isolated polynucleotide for the detection of Enterococcus faecalis, wherein said isolated polynucleotide comprises at least 15 contiguous nucleotides of the nucleic acid sequence of ORF EF122-1, represented by SEQ ID NO:453. The isolated polynucleotide of claim 19, wherein said polynucleotide comprises a heterologous polynucleotide sequence.

21. The isolated polynucleotide of claim 20, wherein said heterologous polynucleotide sequence encodes a heterologous polypeptide.

22. A method for making a recombinant vector comprising inserting the isolated polynucleotide of claim 19 into a vector. 298

23. A nucleic acid sequence complimentary to the polynucleotide of claim 19.

24. A recombinant vector comprising the isolated polynucleotide of claim 19. The recombinant vector of claim 24, wherein said polynucleotide is operably associated with a heterologous regulatory sequence that controls gene expression.

26. A recombinant host cell comprising the isolated polynucleotide of claim 19.

27. The recombinant host cell of claim 26, wherein said polynucleotide is operably associated with a heterologous regulatory sequence that controls gene expression.

28. The isolated polynucleotide of claim 19, wherein said isolated polynucleotide comprises at least 18 contiguous nucleotides of the nucleic acid sequence of ORF EF122-1, represented by SEQ ID NO: 453.

29. The isolated polynucleotide of claim 19, wherein said isolated polynucleotide comprises at least 20 contiguous nucleotides of the nucleic acid sequence of ORF EF122-1, represented by SEQ ID NO: 453.

30. The isolated polynucleotide of claim 29, wherein said isolated polynucleotide comprises at least 40 contiguous nucleotides of the nucleic acid sequence of ORF EF122-1, represented by SEQ ID NO: 453.

31. A method for detecting Enterococcus faecalis using the isolated polynucleotide of claim

32. A method for detecting Enterococcus faecalis using the isolated polynucleotide of claim 19.

33. A method for detecting Enterococcus faecalis using the isolated polynucleotide of claim 28. 299

34. A method for detecting Enterococcus faecalis using the isolated polynucleotide of claim 29. A method for detecting Enterococcus faecalis using the isolated polynucleotide of claim Dated this fifteenth day of February 2002. HUMAN GENOME SCIENCES INC Applicant Wray Associates Perth, Western Australia Patent Attorneys for the Applicant see. 300