AU5588801A - Enterococcus faecalis polynucleotides and polypeptides - Google Patents

Description

P/00/011 28/5/91 Regulation 3.2

AUSTRALIA

Patents Act 1990

ORIGINAL

COMPLETE SPECIFICATION STANDARD PATENT r Name of Applicant: Actual Inventors: Address for service is: Human Genome Sciences, Inc.

Charles A KUNSCH Gil H CHOI Camella BAILEY Alex HROMOCKYJ WRAY ASSOCIATES 239 Adelaide Terrace Perth, WA 6000 Attorney code: WR Invention Title: "Enterococcus faecalis Polynucleotides and Polypeptides" This application is a divisional application by virtue of Section 39 of Australian Patent Application 72793/98 filed on 4 May 1998.

The following statement is a full description of this invention, including the best method of performing it known to me:- 1/2 Enterococcus faecalis polynucleotides and polypeptides Field of the Invention The present invention relates to novel Enterococcusfaecalis genes faecalis) nucleic acids and polypeptides. Also provided are vectors, host cells and recombinant methods for producing the same. Further provided are diagnostic methods for detecting Enterococcusfaecalis using probes, primers, and antibodies to the E. faecalis nucleic acids and polypeptides of the present invention. The invention further relates to screening methods for identifying agonists and antagonists of E. faecalis i( polypeptide activity and to vaccines using E.faecalis nucleic acids and polypeptides.

Background of the Invention Enterococci have been recognized as being pathogenic for humans since the turn of the century when they were first described by Thiercelin in 1988 as microscopic organisms. The genus Enterococcus includes the species Enterococcus faecalis or E. faecalis which is the most common pathogen in the group, accounting for 80 90 percent of all enterococcal infections. See Lewis et al. (1990) Eur J. Clin Microbiol Infect Dis.9: 111-117.

*.o The incidence of enterococcal infections has increased in recent years and enterococci are now the second most frequently reported nosocomial pathogens.

Enterococcal infection is of particular concern because of its resistance to antibiotics.

Recent attention has focused on enterococci not only because of their increasing role in nosocomial infections, but also because of their remarkable and increasing resistance to antimicrobial agents. These factors are mutually reinforcing since resistance allows enterococci to survive in an environment in which antimicrobial agents are heavily used; the hospital setting provides the antibiotics which eliminate or suppress susceptible bacteria, thereby providing a selective advantage for resistant organisms, and the hospital also provides the potential for dissemination of resistant enterococci via the usual routes of hand and environmental contamination.

Antimicrobial resistance can be divided into two general types, inherent or intrinsic property and that which is acquired. The genes for intrinsic resistance, like other species characteristics, appear to reside on the chromosome. Acquired resistance results from either a mutation in the existing DNA or acquisition of new DNA. The various inherent traits expressed by enterococci include resistance to semisynthetic penicillinase-resistant penicillins, cephalosporins, low levels of aminoglycosides, and low levels of clindamycin. Examples of acquired resistance include resistance to chloramphenicol, erythromycin, high levels of clindamycin, tetracycline, high levels of aminoglycosides, penicillin by means of penicillinase, fluoroquinolones, and vancomycin. Resistance to high levels of penicillin without penicillinase and resistance to fluoroquinolones are not known to be plasmid or transposon mediated and presumably are due to mutation(s).

Although the main reservoir for enterococci in humans is the gastrointestinal tract, the bacteria can also reside in the gallbladder, urethra and vagina.

15 E. faecalis has emerged as an important pathogen in endocarditis, bacteremia, urinary tract infections (UTIs), intraabdominal infections, soft tissue infections, and neonatal sepsis. See Lewis et al. (1990) supra.. In the 1970s and 1980s enterococci became firmly established as major nosocomial pathogens. They are now the fourth Sleading cause of hospital-acquired infection and the third leading cause of bacteremia in the United States. Fatality ratios for enterococcal bactermia range from 12% to 68%, with death due to enterococcal sepsis in 4 to 50% of these cases. See T.G. Emori (1993) Clin. Microbiol. Rev. 6:428-442.

The ability of enterococci to colonize the gastrointestinal tract, plus the many intrinsic and acquired resistance traits, means that these organisms, which usually seem to have relatively low intrinsic virulence, are given an excellent opportunity to become secondary invaders. Since nosocomial isolates of enterococci have displayed resistance to essentially every useful antimicrobial agent, it will likely become increasingly difficult to successfully treat and control enterococcal infections.

Particularly when the various resistance genes come together in a single strain, an event almost certain to occur at some time in the future.

The etiology of diseases mediated or exacerbated by Enterococcus faecalis, involves the programmed expression of E.faecalis genes, and that characterizing these genes and their patterns of expression would dramatically add to our understanding of the organism and its host interactions. Knowledge of the E. faecalis gene and genomic organization would improve our understanding of disease etiology and lead to improved and new ways of preventing, treating and diagnosing diseases. Thus, there is a need to characterize the genome of E.faecalis and for polynucleotides of this organism.

S.Summary of the Invention SThe present invention provides for isolated E. faecalis polynucleotides and polypeptides shown in Table 1 and SEQ ID NO: 1 through SEQ ID NO:496 15 (polynucleotide sequences having odd SEQ ID NOs and polypeptide sequences having even SEQ ID NOs). One aspect of the invention provides isolated nucleic acid molecules comprising polynucleotides having a nucleotide sequence selected from the group consisting of: a nucleotide sequence shown in Table 1; a nucleotide S" sequence encoding any of the amino acid sequences of the polypeptides shown in o Table 1; and a nucleotide sequence complementary to any of the nucleotide sequences in or The invention further provides for fragments of the nucleic acid molecules of(a), above.

Further embodiments of the invention include isolated nucleic acid molecules that comprise a polynucleotide having a nucleotide sequence at least 90% identical, and more preferably at least 95%, 96%, 97%, 98% or 99% identical, to any of the nucleotide sequences in or above, or a polynucleotide which hybridizes under stringent hybridization conditions to a polynucleotide in or above.

Additional nucleic acid embodiments of the invention relate to isolated nucleic acid molecules comprising polynucleotides which encode the amino acid sequences of epitope-bearing portions of a E. faecalis polypeptide having an amino acid sequence in above.

The present invention also relates to recombinant vectors, which include the isolated nucleic acid molecules of the present invention, and to host cells containing the recombinant vectors, as well as to methods of making such vectors and host cells.

The present invention further relates to the use of these vectors in the production of E. faecalis polypeptides or peptides by recombinant techniques.

The invention further provides isolated E. faecalis polypeptides having an amino acid sequence selected from the group consisting of an amino acid sequence of any of the polypeptides described in Table 1 or fragments thereof.

The polypeptides of the present invention also include polypeptides having an amino acid sequence with at least 70% similarity, and more preferably at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% similarity to those described in Table 1, as well as polypeptides having an amino acid sequence at least 70% identical, more 15 preferably at least 75% identical, and still more preferably 80%, 85%, 90%, 96%, 97%, 98%, or 99% identical to those above; as well as isolated nucleic acid molecules encoding such polypeptides.

The present invention further provides a single or multi-component vaccine comprising one or more of the E. faecalis polynucleotides or polypeptides described in Table 1, or fragments thereof, together with a pharmaceutically acceptable diluent, carrier, or excipient, wherein the E. faecalis polypeptide(s) are present in an amount effective to elicit an immune response to members of the Enterococcus genus, or at least E. faecalis in an animal. The E. faecalis polypeptides of the present invention may further be combined with one or more immunogens of one or more other Enterococcal or non-Enterococcal organisms to produce a multi-component vaccine intended to elicit an immunological response against members of the Enterococcus genus and, optionally, one or more non-Enterococcal organisms.

The vaccines of the present invention can be administered in a DNA form, e.g., "naked" DNA, wherein the DNA encodes one or more Enterococcal polypeptides and, optionally, one or more polypeptides of a non-Enterococcal organism. The DNA encoding one or more polypeptides may be constructed such that these polypeptides are expressed as fusion proteins.

The vaccines of the present invention may also be administered as a component of a genetically engineered organism or host cell. Thus, a genetically engineered organism or host cell which expresses one or more E. faecalis polypeptides may be administered to an animal. For example, such a genetically engineered organism or host cell may contain one or more E. faecalis polypeptides of the present invention intracellularly, on its cell surface, or in its periplasmic space. Further, such a genetically engineered organism or host cell may secrete one or more E. faecalis polypeptides. The vaccines of the present invention may also be co-administered to an animal with an immune system modulator CD86 and GM-CSF).

S. The invention also provides a method of inducing an immunological response S* in an animal to one or more members of the Enterococcus genus, preferably one or 15 more isolates of the E.faecalis species, comprising administering to the animal a vaccine as described above.

The invention further provides a method of inducing a protective immune response in an animal, sufficient to prevent, attenuate, or control an infection by members of the Enterococcus genus, preferably at least E. faecalis species, 20 comprising administering to the animal a composition comprising one or more of the polynucleotides or polypeptides described in Table 1, or fragments thereof. Further, these polypeptides, or fragments thereof, may be conjugated to another immunogen and/or administered in admixture with an adjuvant.

The invention further relates to antibodies elicited in an animal by the administration of one or more E. faecalis polypeptides of the present invention and to methods for producing such antibodies and fragments thereof. The invention further relates to recombinant antibodies and fragments thereof and to methods for producing such antibodies and fragments thereof.

The invention also provides diagnostic methods for detecting the expression of the polynucleotides of Table 1 by members of the Enterococcus genus in an animal.

One such method involves assaying for the expression of a polynucleotide encoding E. faecalis polypeptides in a sample from an animal. This expression may be assayed either directly by assaying polypeptide levels using antibodies elicited in response to amino acid sequences described in Table 1) or indirectly by assaying for antibodies having specificity for amino acid sequences described in Table The expression of polynucleotides can also be assayed by detecting the nucleic acids of Table 1. An example of such a method involves the use of the polymerase chain reaction (PCR) to amplify and detect Enterococcus nucleic acid sequences.

The present invention also relates to nucleic acid probes having all or part of a nucleotide sequence described in Table 1 (odd SEQ ID NOs) which are capable of hybridizing under stringent conditions to Enterococcus nucleic acids. The invention further relates to a method of detecting one or more Enterococcus nucleic acids in a biological sample obtained from an animal, said one or more nucleic acids encoding 15 Enterococcus polypeptides, comprising: contacting the sample with one or more of the above-described nucleic acid probes, under conditions such that hybridization occurs, and detecting hybridization of said one or more probes to the Enterococcus nucleic acid present in the biological sample.

Other uses of the polypeptides of the present invention include: inter alia, to detect E. faeca.s in immunoassays, as epitope tags, as molecular weight markers on SDS-PAGE gels, as molecular weight markers for molecular sieve gel filtration columns, to generate antibodies that specificaly bind E. faecalis polypeotides of the present invention for the detection E. faecalis in immunoassays, to generate an immune response against E. faecalis and other Enterococcs species, and as vaccines against E. faecalis, other Enterococcus species and other bacteria genuses.

Isolated nucleic acid molecules of the present invention, particularly DNA molecules, are useful as probes for gene mapping and for identifying E. faecalis in a biological samples, for instance, by Southern and Northern blot analysis.

Polynucleotides of the present invention are also useful in detecting E.faecalis by PCR using primers for a particular E. faecalis polynucleotide. Isolated polynucleotides of the present invention are also useful in making the polypeptides of the present invention.

Detailed Description The present invention relates to recombinant E. faecalis nucleic acids and fragments thereof The present invention further relates to recombinant E. faecalis polypeptides and fragments thereof. The invention also relates to methods for using these polypeptides to produce immunological responses aid to confer immunological In protection to disease caused by members of the genus Enterococcus, at least isolates of the E. faecalis genus. The invention further relates to nucleic acid sequences which encode antigenic E. faecalis polypeptides and to methods for detecting E. faecalis nucleic acids and polypeptides in biological samples. The invention also relates to antibodies specific for the polypeptides and peptides of the present invention and 15 methods for detecting such antibodies produced in a host animal.

Definitions The following definitions are provided to clarify the subject matter which the inventors consider to be the present invention.

20 As used herein, the phrase "pathogenic agent" means an agent which causes a disease state or affliction in an animal. Included within this definition, for examples, are bacteria, protozoans, fungi, viruses and metazoan parasites which either produce a disease state or render an animal infected with such an organism susceptible to a disease state a secondary infection). Further included are species and strains of the genus Enterococcus which produce disease states in animals.

As used herein, the term "organism" means any living biological system, including viruses, regardless of whether it is a pathogenic agent.

As used herein, the term "Enlerococcus" means any species or strain of bacteria which is members of the genus Enterococcus. Such species and strains are known to those of skill in the art, and include those that are pathogenic and those that are not.

As used herein, the phrase "one or more E. faecalis polypeptides of the present invention" means polypeptides comprising the amino acid sequence of one or more of the E. faecalis polypeptides described in Table 1 (even SEQ ID NOs). These polypeptides may be expressed as fusion proteins wherein the E. faecalis polypeptides of the present invention are linked to additional amino acid sequences which may be of Enterococcal or non-Enterococcal origin. This phrase further includes polypeptide comprising fragments of the E. faecalis polypeptides of the 1( present invention. Additional definitions are provided throughout the specification.

Explanation of Table 1 Table 1, below, provides information describing genes which encode polypeptides of E.faecalis. The table lists the gene identifier which consists of the 15 letters EF, which denote E.faecalis, followed imrnediately by a three digit numeric code, which arbitrarily number the E.faecalis genes of the present invention. A number from 1 through 4 follows the three digit number. A number 1 represents the full length open reading frame of the gene specified by the preceeding three digit number. A number 2 represents the full length polypeptide encoded by the gene specified the preceeding three digit number. A number 3 represents a polynucleotide fragment, of the gene represented by the preceeding three digit number, used to produce an antigenic polypeptide. A number 4 represents an antigenic polypeptide fragment, of the gene represented by the preceeding three digit number, used to stimulate an immune response or as a vaccine. The nucleotide and amino acid sequences of each gene and fragment are also shown in the Sequence Listing under the SEQ ID NO listed in Table 1.

Explanation of Table 2 Table 2 lists accession numbers for the closest matching sequences between the polypeptides of the present invention and those available through GenBank and Derwent databases. These reference numbers are the database entry numbers commonly used by those of skill in the art, who will be familar with their denominations. The descriptions of the numenclature for GenBank are available from the National Center for Biotechnology Information. Column 1 lists the gene or ORF of the present invention. Column 2 lists the accession number of a "match" gene sequence in GenBank or Derwent databases. Column 3 lists the description of the "match" gene sequence. Columns 4 and 5 are the high score and smallest sum probability, respectively, calculated by BLAST. Polypeptides of the present invention that do not share significant identity/similarity with any polypeptide sequences of GenBank and Derwent are not represented in Table 2. Polypeptides of the present invention that share significant identity/similarity with more than one of the polypeptides of GenBank and Derwent are represented more than once.

15 Explanation of Table 3.

The E. faecalis polypeptides of the present invention may include one or more S•conservative amino acid substitutions from natural mutations or human manipulation as indicated in Table 3. Changes are preferably of a minor nature, such as conservative amino acid substitutions that do not significantly affect the folding or activity of the protein. Residues from the following groups, as indicated in Table 3, may be substituted for one another: Aromatic, Hydrophobic, Polar, Basic, Acidic, and Small,

S

Explanation of Table 4 Table 4 lists residues comprising antigenic epitopes of antigenic epitopebearing fragments present in each of the full length E. faecalis polypeptides described in Table I as predicted by the inventors using the algorithm of Jameson and Wolf, (1988) Comp. Appl. Biosci. 4:181-186. The Jameson-Wolf antigenic analysis was performed using the computer program PROTEAN (Version 3.11 for the Power Macintosh, DNASTAR, Inc., 1228 South Park Street Madison, WI). E. faecalis polypeptide shown in Table 1 may one or more antigenic epitopes comprising residues described in Table 4. It will be appreciated that depending on the analytical criteria used to predict antigenic determinants, the exact address of the determinant may vary slightly. The residues and locations shown described in Table 4 correspond to the amino acid sequences for each full length gene sequence shown in Table I and in the Sequence Listing. Polypeptides of the present invention that do not have antigenic epitopes recognized by the Jameson-Wolf algorithm are not represented in Table 2.

Selection of Nucleic Acid Sequences Encoding Antigenic E. faecalis Polypeptides Sequenced E. faecalis genomic DNA was obtained from the E. faecalis strain V586. The E. faecalis strain V586 was deposited 2 May 1997 at the ATCC, 10801 University Blvd. Manassas, VA 20110-2209, and given accession number 55969.

Some ORFs contained in the subset of fragments of the E. faecalis genome 15 disclosed herein were derived through the use of a number of screening criteria detailed below. The ORFs are bounded at the amino terminus by a methionine or valine residue and usually at the carboxy terminus by a stop codon.

*ooo Most of the selected sequences consist of complete ORFs. The polypeptides that do not comprise a complete ORF can be determined by determining whether the 20 corresponding polynucleotide sequence comprises a stop codon after the codon for the last amino acid residue in the polypeptide sequence. It is not always preferred to express a complete ORF in a heterologous system. It may be challenging to express and purify a highly hydrophobic protein by common laboratory methods. Some of the polypeptide vaccine candidates described herein have been modified slightly to simplify the production of recombinant protein. For example, nucleotide sequences which encode highly hydrophobic domains, such as those found at the amino terminal signal sequence, have been excluded from some constructs used for expression of the polypeptides. Furthermore, any highly hydrophobic amino acid sequences occurring at the carboxy terminus have also been excluded from the recombinant expression constructs. Thus, in one embodiment, a polypeptide which represents a truncated or modified ORF may be used as an antigen.

While numerous methods are known in the art for selecting potentially immunogenic polypeptides, many of the ORFs disclosed herein were selected on the basis of screening Enterococcusfaecalis ORFs for several aspects of potential immnunogenicity. One set of selection criteria are as follows: 1. Type I signal sequence: An amino terminal type I signal sequence generally directs a nascent protein across the plasma and outer membranes to the exterior of the bacterial cell. Experimental evidence obtained from studies with Escherichia coli suggests that the typical type I signal sequence consists of the following biochemical and physical attributes (Izard, J. W. and Kendall, D. A. Mol. Microbiol. 13:765-773 (1994)). The length of the type I signal sequence is approximately 15 to 25 primarily hydrophobic amino acid residues with a net positive charge in the extreme amino terminus. In addition, the central region of the signal sequence adopts an alpha-helical conformation in a hydrophobic environment. Finally, the region surrounding the actual site of cleavage is ideally six residues long, with small side-chain amino acids in the -1 and -3 positions.

2. Type V signal sequence: The type IV signal sequence is an example of the several types of functional signal sequences which exist in addition to the type I signal sequence detailed above. Although functionally related, the type IV signal sequence possesses a unique set of biochemical and physical attributes (Strom, M. S. and Lory, J. Bacteriol. 174:7345-7351 (1992)). These are typically six to eight amino acids with a net basic charge followed by an additional sixteen to thirty primarily hydrophobic residues. The cleavage site of a type IV signal sequence is typically after the initial six to eight amino acids at the extreme amino terminus. In addition, type IV signal sequences generally contain a phenylalanine residue at the +1 site relative to the cleavage site.

3. Lipoprotein: Studies of the cleavage sites of twenty-six bacterial lipoprotein precursors has allowed the definition of a consensus amino acid sequence for lipoprotein cleavage. Nearly three-fourths of the bacterial lipoprotein precursors examined contained the sequence at positions -3 to relative to the point of cleavage (Hayashi, S. and Wu, H. Bioenerg. Biomembr. 22:451-471 (1990)).

4. LPXTG motif- It has been experimentally determined that most anchored proteins found on the surface of gram-positive bacteria possess a highly conserved carboxy terminal sequence. More than fifty such proteins from organisms such as S.

pyogenes, S. mutans, E. faecalis, S. pneumoniae, and others, have been identified based on their extracellular location and carboxy terminal amino 'acid sequence (Fischetti, V.

ASMNews 62:405-410 (1996)). The conserved region consists of six charged amino acids at the extreme carboxy terminus coupled to 15-20 hydrophobic amino acids presumed to function as a transmembrane domain. Immediately adjacent to the transmembrane domain is a six amino acid sequence conserved in nearly all proteins examined. The amino acid sequence of this region is L-P-X-T-G-X, where X is any 15 amino acid.

An algorithm for selecting antigenic and immunogenic Enterococcusfaecalis polypeptides including the foregoing criteria was developed. The algorithm is similar to that described in U.S. patent application 08/781,986, filed January 3, 1997, which is fully incorporated by reference herein. Use of the algorithm by the inventors to select immunologically useful Enterococcusfaecalis polypeptides resulted in the selection of a number of the disclosed ORFs. Polypeptides comprising the polypeptides identified in this group may be produced by techniques standard in the art and as further described herein.

Nucleic Acid Molecules Sequenced E. faecalis genomic DNA was obtained from the E. faecalis strainV586. As discussed elsewhere hererin, polynucleotides of the present invention readily may be obtained by routine application of well known and standard procedures for cloning and sequencing DNA. Detailed methods for obtaining libraries and for sequencing are provided below, for instance. A wide variety of Enierococcusfaecalis strains that can be used to prepare E.faecalis genomic DNA for cloning and for obtaining polynucleotides and polypeptides of the present invention. A wide variety of Enmerococcusfaecalis strains are available to the public from recognized depository institutions, such as the American Type Culture Collection (ATCC). It is recognized that minor variation is the nucleic acid and amino acid sequence may be expected from Efaecalis strain to strain. The present invention provides for genes, including both polynucleotides and polypeptides, of the of the present invention from all the Enterococcusfaecalis strains.

Unless otherwise indicated, all nucleotide sequences determined by sequencing a DNA molecule herein were determined using an automated DNA sequencer (such as the Model 373 from Applied Biosystems, Inc., Foster City, CA), and all amino acid sequences of polypeptides encoded by DNA molecules determined herein were predicted by translation of a DNA sequence determined as above. Therefore, as is known in the art for any DNA sequence determined by this automated approach, any S nucleotide sequence determined herein may contain some errors. Nucleotide sequences detennined by automation are typically at least about 90% identical, more typically at least about 95% to at least about 99.9% identical to the actual nucleotide sequence of the sequenced DNA molecule. The actual sequence can be more 20 precisely determined by other approaches including manual DNA sequencing methods well known in the art. As is also known in the art, a single insertion or deletion in a determined nucleotide sequence compared to the actual sequence will cause a frame shift in translation of the nucleotide sequence such that the predicted amino acid sequence encoded by a determined nucleotide sequence will be completely different from the amino acid sequence actually encoded by the sequenced DNA molecule, beginning at the point of such an insertion or deletion. In case of conflict between Table 1 and either the nucleic acid sequence of the clones listed in Table 1 or the amino acid sequence of the protein expressed by the clones listed in Table 1, the clones listed in Table 1 are controlling. By "nucleotide sequence" of a nucleic acid molecule or polynucleotide is intended to mean either a DNA or RNA sequence.Using the information provided herein, such as the nucleotide sequence in Table 1, a nucleic acid molecule of the present invention encoding a E. faecalis polypeptide may be obtained using standard cloning and screening procedures, such as those for cloning DNAs using genomic DNA as starting material. See, Sambrook et al. MOLECULAR CLONING: A LABORATORY MANUAL (Cold Spring Harbor, N.Y. 2nd ed.

1989); Ausubel et al., CURRENT PROTOCALS IN MOLECULAR BIOLOGY (John Wiley and Sons, N.Y. 1989). Illustrative of the invention, the nucleic acid molecule described in Table 1 was discovered in a DNA library derived from a E.

faecalis genomic DNA.

Nucleic acid molecules of the present invention may be in the form of RNA, such as mRNA, or in the form of DNA, including, for instance, DNA and genomic DNA obtained by cloning or produced synthetically. The DNA may be double-stranded or single-stranded. Single-stranded DNA or RNA may be the coding 15 strand, also known as the sense strand, or it may be the non-coding strand, also referred to as the anti-sense strand.

By "isolated" nucleic acid molecule(s) is intended a nucleic acid molecule, DNA or RNA, which has been removed from its native environment. This includes segments of DNA comprising the E. faecalis polynucleotides of the present invention isolated from the native chromosome. These fragments include both isolated fragments consisting only of E. faecalis DNA and fragments comprising heterologous sequences such as vector sequences or other foreign DNA. For example, recombinant DNA molecules contained in a vector are considered isolated for the purposes of the present invention. Further examples of isolated DNA molecules include recombinant DNA molecules maintained in heterologous host cells or purified (partially or substantially) DNA molecules in solution. Isolated RNA molecules include in vivo or in vitro RNA transcripts of the DNA molecules of the present invention. Isolated nucleic acid molecules according to the present invention further include such molecules produced synthetically.

In addition, isolated nucleic acid molecules of the invention include DNA molecules which comprise a sequence substantially different from those described above but which, due to the degeneracy of the genetic code, still encode a E. faecalis polypeptides and peptides of the present invention polypeptides of Table 1).

That is, all possible DNA sequences that encode the E. faecalis polypeptides of the present invention. This includes the genetic code and species-specific codon preferences known in the art. Thus, it would be routine for one skilled in the art to generate the degenerate variants described above, for instance, to optimize codon expression for a particular host change codons in the bacteria mRNA to those preferred by a mammalian or other bacterial host such as E. coli).

The invention further provides isolated nucleic acid molecules having the nucleotide sequence shown in Table 1 or a nucleic acid molecule having a sequence complementary to one of the above sequences. Such isolated molecules, particularly DNA molecules, are useful as probes for gene mapping and for identifying E.faecalis in a biological sample, for instance, by PCR, Southern blot, Northern blot, or other Sform of hybridization analysis.

The present invention is further directed to nucleic acid molecules encoding portions or fragments of the nucleotide sequences described herein. Fragments include portions of the nucleotide sequences of Table 1, or the E. faecalis nucleotide sequences contained in the plasimd clones listed in Table 1, at least 10 contiguous nucleotides in length selected from any two integers, one of which representing a nucleotide position and a second of which representing a 3' nucleotide position, where the first nucleotide for each nucleotide sequence in Table 1 is position 1. That is, every combination of a 5' and 3' nucleotide position that a fragment at least contiguous nucleotides in length could occupy is included in the invention. At least means a fragment may be 10 contiguous nucleotide bases in length or any integer between 10 and the length of an entire nucleotide sequence of Table 1 minus 1.

Therefore, included in the invention are contiguous fragments specified by any 5' and 3' nucleotide base positions of a nucleotide sequences of Table 1 wherein the contiguous fragment is any integer between 10 and the length of an entire nucleotide sequence minus 1.

Further, the invention includes polynucleotides comprising fragments specified by size, in nucleotides, rather than by nucleotide positions. The invention includes any fragment size, in contiguous nucleotides, selected from integers between 10 and the length of an entire nucleotide sequence minus 1. Preferred sizes of contiguous nucleotide fragments include 20 nucleotides, 30 nucleotides, 40 nucleotides, nucleotides. Other preferred sizes of contiguous nucleotide fragments, which may be useful as diagnostic probes and primers, include fragments 50-300 nucleotides in length which include, as discussed above, fragment sizes representing each integer between 50-300. Larger fragments are also useful according to the present invention corresponding to most, if not all, of the nucleotide sequences shown in Table 1or of the E. faecalis nucleotide sequences of the plasimd clones listed in Table 1. The preferred sizes are, of course, meant to exemplify not limit the present invention as all size fragments, representing any integer between 10 and the length of an entire nucleotide sequence minus 1, are included in the invention. Additional preferred nucleic acid fragments of the present invention include nucleic acid molecules encoding epitope-bearing portions of E.faecalis polypeptides identified in Table 4.

The present invention also provides for the exclusion of any fragment, specified by 5' and 3' base positions or by size in nucleotide bases as described above for any nucleotide sequence of Table 1 or the plasimd clones listed in Table 1. Any number of fragments of nucleotide sequences in Table 1 or the plasimd clones listed in Table 1, specified by 5' and 3' base positions or by size in nucleotides, as described above, may be excluded from the present invention.

In another aspect, the invention provides an isolated nucleic acid molecule comprising a polynucleotide which hybridizes under stringent hybridization conditions to a portion of a polynucleotide in a nucleic acid molecules of the invention described above, for instance, nucleotide sequences of Table 1 or the E. faecalis sequences of the plasimd clones listed in Table 1. By "stringent hybridization conditions" is intended overnight incubation at 42 0 C in a solution comprising: fornamide, 5x SSC (150 mM NaC1, 15 mM trisodium citrate), 50 mM sodium phosphate (pH 5x Denhardt's solution, 10% dextran sulfate, and 20 p-g/ml denatured, sheared salmon sperm DNA, followed by washing the filters in 0. Ix SSC at about 65 0

C.

By a polynucleotide which hybridizes to a "portion" of a polynucleotide is intended a polynucleotide (either DNA or RNA) hybridizing to at least about nucleotides bases, and more preferably ai least about 20 nucleotides bases, still more preferably at least about 30 nucleotides bases, and even more preferably about 30-70 50) nucleotides bases of the reference polynucleotide. These are useful as diagnostic probes and primers as discussed above. By a portion of a polynucleotide of "at least 20 nucleotides bases in length," for example, is intended 20 or more contiguous nucleotides bases nucleotides from the nucleotide sequence of the reference polynucleotide the nucleotide sequence as shown in Table Portions of a polynucleotide which hybridizes to a nucleotide sequence in Table 1, which can be Sused as probes and primers, may also be precisely specified by 5' and 3' base positions or by size in nucleotide bases as described above or precisely excluded in the same manner.

The nucleic acid molecules of the present invention include those encoding the full length E. faecalis polypeptides of Table 1 and portions of the E. faecalis polypeptides of Table 1. Also included in the present invention are nucleic acids encoding the above full length sequences and further comprise additional sequences, such as those encoding an added secretory leader sequence, such as a pre-, or pro- or prepro- protein sequence. Further included in the present invention are nucleic acids encoding the above full length sequences and portions thereof and further comprise additional heterologous amino acid sequences encoded by nucleic acid sequences from a different source.

Also included in the present invention are nucleic acids encoding the above protein sequences together with additional, non-coding sequences, including for example, but not limited to non-coding 5' and 3' sequences. These sequences include transcribed, non-translated sequences that may play a role in transcription, and mRNA processing, for example, ribosome binding and stability of mRNA. Also included in the present invention are additional coding sequences which provide additional functionalities.

Thus, a nucleotide sequence encoding a polypeptide may be fused to a marker sequence, such as a sequence encoding a peptide which facilitates purification of the fused polypeptide. In certain preferred embodiments of this aspect of the invention, the marker amino acid sequence is a hexa-histidine peptidd, such as the tag provided in a pQE vector (QIAGEN, Inc., 9259 Eton Avenue, Chatsworth, CA, 91311), among others, many of which are commercially available. For instance, hexa-histidine provides for convenient purification of the fusion protein. See Gentz et al. (1989) Proc. Natl. Acad. Sci. 86:821-24. The "HA" tag is another peptide useful for purification which corresponds to an epitope derived from the influenza hemagglutinin protein. See Wilson et al. (1984) Cell 37:767. As discussed below, other such fusion proteins include the E. faecalis polypeptides of the present invention fused to Fc at the N- or C-terminus.

Variant and Mutant Polvnucleotides The present invention further relates to variants of the nucleic acid molecules which encode portions, analogs or derivatives of a E. faecalis polypeptides of Table 1 and variant polypeptides thereof including portions, analogs, and derivatives of the E.

faecalis polypeptides. Variants may occur naturally, such as a natural allelic variant.

By an "allelic variant" is intended one of several alternate forms of a gene occupying a given locus on a chromosome of an organism. See, B. Lewin, Genes IV (1990).

Non-naturally occurring variants may be produced using art-known mutagenesis techniques.

Such nucleic acid variants include those produced by nucleotide substitutions, deletions, or additions. The substitutions, deletions, or additions may involve one or more nucleotides. The variants may be altered in coding regions, non-coding regions, or both. Alterations in the coding regions may produce conservative or non-conservative amino acid substitutions, deletions or additions. Especially preferred among these are silent substitutions, additions and deletions, which do not alter the properties and activities of a E. faecalis protein of the present invention or portions thereof. Also especially preferred in this regard are conservative substitutions.

Such polypeptide variants include those produced by amino acid substitutions, deletions or additions. The substitutions, deletions, or additions may involve one or more residues. Alterations may produce conservative or non-conservative amino acid substitutions, deletions, or additions. Especially preferred among these are silent substitutions, additions and deletions, which do not alter the properties and activities of a E. faecalis protein of the present invention or portions thereof. Also especially preferred in this regard are conservative substitutions.

The present invention also relates to recombinant vectors, which include the isolated nucleic acid molecules of the present invention, and to host cells containing the recombinant vectors, as well as to methods of making such vectors and host cells S* and for using them for production ofE. faecalis polypeptides or peptides by recombinant techniques.

The present application is directed to nucleic acid molecules at least 2* 95%, 96%, 97%, 98% or 99% identical to a nucleic acid sequence shown in Table 1.

The above nucleic acid sequences are included irrespective of whether they encode a polypeptide having E. faecalis activity. This is because even where a particular nucleic acid molecule does not encode a polypeptide having E. faecalis activity, one of skill in the art would still know how to use the nucleic acid molecule, for instance, as a hybridization probe. Uses of the nucleic acid molecules of the present invention that do not encode a polypeptide having E. faecalis activity include, inter alia, isolating an E. faecalis gene or allelic variants thereof from a DNA library, and detecting E. faecalis mRNA expression samples, environmental samples, suspected of containing E.

faecalis by Northern Blot analysis.

Preferred, are nucleic acid molecules having sequences at least 90%, 95%, 96%, 97%, 98% or 99% identical to the nucleic acid sequence shown in Table 1, which do, in fact, encode a polypeptide having E. faecalis protein activity By "a polypeptide having E. faecalis activity" is intended polypeptides exhibiting activity similar, but not necessarily identical, to an activity of the E. faecalis protein of the invention, as measured in a particular biological assay suitable for measuring activity of the specified protein.

Due to the degeneracy of the genetic code, one of ordinary skill in the art will immediately recognize that a large number of the nucleic acid molecules having a sequence at least 90%, 95%, 96%, 97%, 98%, or 99% identical to the nucleic acid sequences shown in Table 1 will encode a polypeptide having E. faecalis protein activity. In fact, since degenerate variants of these nucleotide sequences all encode the same polypeptide, this will be clear to the skilled artisan even without performing the above described comparison assay. It will be further recognized in the art that, for such nucleic acid molecules that are not degenerate variants, a reasonable number will also encode a polypeptide having E. faecalis protein activity. This is because the skilled artisan is fully aware of amino acid substitutions that are either less likely or *o*o not likely to significantly effect protein function replacing one aliphatic amino acid with a second aliphatic amino acid), as further described below.

The biological activity or function of the polypeptides of the present invention are expected to be similar or identical to polypeptides from other bacteria that share a high degree of structural identity/similarity. Tables 2 lists accession numbers and descriptions for the closest matching sequences of polypeptides available through Genbank and Derwent databases. It is therefore expected that the biological activity or function of the polypeptides of the present invention will be similar or identical to those polypeptides from other bacterial genuses, species, or strains listed in Table 2.

By a polynucleotide having a nucleotide sequence at least, for example, "identical" to a reference nucleotide sequence of the present invention, it is intended that the nucleotide sequence of the polynucleotide is identical to the reference sequence except that the polynucleotide sequence may include up to five point mutations per each 100 nucleotides of the reference nucleotide sequence encoding the E. faecalis polypeptide. In other words, to obtain a polynucleotide having a nucleotide sequence at least 95% identical to a reference nucleotide sequence, up to of the nucleotides in the reference sequence may be deleted, inserted, or substituted with another nucleotide. The query sequencelnmay be an entire sequence 1o shown in Table 1, the ORF (open reading frame), or any fragment specified as described herein.

As a practical matter, whether any particular nucleic acid molecule or polypeptide is at least 90%, 95%, 96%, 97%, 98% or 99% identical to a nucleotide sequence of the presence invention can be determined conventionally using known 15 computer programs. A preferred method for determining the best overall match between a query sequence (a sequence of the present invention) and a subject sequence, also referred to as a global sequence alignment, can be determined using the FASTDB computer program based on the algorithm of Brutlag et al. See Brutlag et al. (1990) Comp. App. Biosci. 6:237-245. In a sequence alignment the query and subject sequences are both DNA sequences. An RNA sequence can be compared by first converting U's to T's. The result of said global sequence alignment is in percent identity. Preferred parameters used in a FASTDB alignment of DNA sequences to calculate percent identity are: Matrix=Unitary, k-tuple=4, Mismatch Penalty=l, Joining Penalty=30, Randomization Group Length=0, Cutoff Score=l, Gap Penalty=5, Gap Size Penalty 0.05, Window Size=500 or the lenght of the subject nucleotide sequence, whichever is shorter.

If the subject sequence is shorter than the query sequence because of 5' or 3' deletions, not because of internal deletions, a manual correction must be made to the results. This is because the FASTDB program does not account for 5' and 3' truncations of the subject sequence when calculating percent identity. For subject sequences truncated at the 5' or 3' ends, relative to the query sequence, the percent identity is corrected by calculating the number of bases of the query sequence that are and 3' of the subject sequence, which are not matched/aligned, as a percent of the total bases of the query sequence. Whether a nucleotide is matched/aligned is determined by results of the FASTDB sequence alignment. This percentage is then subtracted from the percent identity, calculated by the above FASTDB program using the specified parameters, to arrive at a final percent identity score. This corrected score is what is used for the purposes of the present invention. Only nucleotides to outside the 5' and 3' nucleotides of the subject sequence, as displayed by the FASTDB alignment, which are not matched/aligned with the query sequence, are calculated for the purposes of manually adjusting the percent identity score.

For example, a 90 nucleotide subject sequence is aligned to a 100 nucleotide query sequence to determine percent identity. The deletions occur at the 5' end of the 15 subject sequence and therefore, the FASTDB alignment does not show a matched/alignment of the first 10 nucleotides at 5' end. The 10 unpaired nucleotides represent 10% of the sequence (number of nucleotides at the 5' and 3' ends not matched/total number of nucleotides in the query sequence) so 10% is subtracted from the percent identity score calculated by the FASTDB program. If the remaining nucleotides were perfectly matched the final percent identity would be 90%. In another example, a 90 nucleotide subject sequence is compared with a 100 nucleotide query sequence. This time the deletions are internal deletions so that there are no nucleotides on the 5' or 3' of the subject sequence which are not matched/aligned with the query. In this case the percent identity calculated by FASTDB is not manually corrected. Once again, only nucleotides 5' and 3' of the subject sequence which are not matched/aligned with the query sequence are manually corrected for. No other manual corrections are to made for the purposes of the present invention.

Vectors and Host Cell The present invention also relates to vectors which include the isolated DNA molecules of the present invention, host cells comprising the recombinant vectors, and the production of E. faecalis polypeptides and peptides of the present invention expressed by the host cells.

Recombinant constructs may be introduced into host cells using well known techniques such as infection, transduction, transfection, transvection, electroporation and transformation. The vector may be, for example, a phage, plasmid, viral or retroviral vector. Retroviral vectors may be replication competent or replication defective. In the latter case, viral propagation generally will occur only in complementing host cells.

The polynucleotides may be joined to a vector containing a selectable marker for propagation in a host. Generally, a plasmid vector is introduced in a precipitate, such as a calcium phosphate precipitate, or in a complex with a charged lipid. If the vector is a virus, it may be packaged in vitro using an appropriate packaging cell line 15 and then transduced into host cells.

S* Preferred are vectors comprising cis-acting control regions to the polynucleotide of interest. Appropriate trans-acting factors may be supplied by the host, supplied by a complementing vector or supplied by the vector itself upon introduction into the host.

In certain preferred embodiments in this regard, the vectors provide for specific expression, which may be inducible and/or cell type-specific. Particularly preferred among such vectors are those inducible by environmental factors that are easy to manipulate, such as temperature and nutrient additives.

Expression vectors useful in the present invention include chromosomal-, episomal- and virus-derived vectors, vectors derived from bacterial plasmids, bacteriophage, yeast episomes, yeast chromosomal elements, viruses such as baculoviruses, papova viruses, vaccinia viruses, adenoviruses, fowl pox viruses, pseudorabies viruses and retroviruses, and vectors derived from combinations thereof, such as cosmids and phagemids.

The DNA insert should be operatively linked to an appropriate promoter, such as the phage lambda PL promoter, the E. coli lac, trp and tac promoters, the early and late promoters and promoters of retroviral LTRs, to name a few.

Other suitable promoters will be known to the skilled artisan. The expression constructs will further contain sites for transcription initiation, termination and, in the transcribed region, a ribosome binding site for translation. The coding portion of the mature transcripts expressed by the constructs will preferably include a translation initiating site at the beginning and a termination codon (UAA, UGA or UAG) appropriately positioned at the end of the polypeptide to:be translated.

As indicated, the expression vectors will preferably include at least one selectable marker. Such markers include dihydrofolate reductase or neomycin resistance for eukaryotic cell culture and tetracycline, kanamycin, or ampicillin resistance genes for culturing in E. coli and other bacteria. Representative examples of appropriate hosts include, but are not limited to, bacterial cells, such as E. coli, 15 Streptomyces and Salmonella typhimurium cells; fungal cells, such as yeast cells; insect cells such as Drosophila S2 and Spodoptera Sf9 cells; animal cells such as CHO, COS and Bowes melanoma cells; and plant cells. Appropriate culture mediums and conditions for the above-described host cells are known in the art.

Among vectors preferred for use in bacteria include pQE70, pQE60 and pQE9, 20 pQE 10 available from Qiagen; pBS vectors, Phagescript vectors, Bluescript vectors, pNHSA, pNH 16a, pNH18A, pNH46A available from Stratagene; pET series of vectors available from Novagen; and ptrc99a, pKK223-3, pKK233-3, pDR540, available from Pharmacia. Among preferred eukaryotic vectors are pWLNEO, pSV2CAT, pOG44, pXTI and pSG available from Stratagene; and pSVK3, pBPV, 25 pMSG and pSVL available from Pharmacia. Other suitable vectors will be readily apparent to the skilled artisan.

Among known bacterial promoters suitable for use in the present invention include the E. coli lacI and lacZ promoters, the T3, T5 and T7 promoters, the gpt promoter, the lambda PR and PL promoters and the trp promoter. Suitable eukaryotic promoters include the CMV immediate early promoter, the HSV thymidine kinase promoter, the early and late SV40 promoters, the promoters of retroviral LTRs, such as those of the Rous sarcoma virus (RSV), and metallothionein promoters, such as the mouse metallothioriein-I promoter.

Introduction of the construct into the host cell can be effected by calcium phosphate transfection, DEAE-dextran mediated transfection, cationic lipid-mediated transfection, electroporation, transduction, infection or other methods. Such methods are described in many standard laboratory manuals (for example, Davis, et al., Basic Methods In Molecular Biology (1986)).

Transcription of DNA encoding the polypeptides of the present invention by higher eukaryotes may be increased by inserting an enhancer sequence into the vector.

Enhancers are cis-acting elements of DNA, usually about from 10 to 300 nucleotides that act to increase transcriptional activity of a promoter in a given host cell-type.

'Examples of enhancers include the SV40 enhancer, which is located on the late side of 15 the replication origin at nucleotides 100 to 270, the cytomegalovirus early promoter enhancer, the polyoma enhancer on the late side of the replication origin, and adenovirus enhancers.

For secretion of the translated polypeptide into the lumen of the endoplasmic reticulum, into the periplasmic space or into the extracellular environment, appropriate secretion signals may be incorporated into the expressed polypeptide, for example, the amino acid sequence KDEL. The signals may be endogenous to the polypeptide or they may be heterologous signals.

The polypeptide may be expressed in a modified form, such as a fusion ***protein, and may include not only secretion signals, but also additional heterologous functional regions. For instance, a region of additional amino acids, particularly charged amino acids, may be added to the N-terminus of the polypeptide to improve stability and persistence in the host cell, during purification, or during subsequent handling and storage. Also, peptide moieties may be added to the polypeptide to facilitate purification. Such regions may be removed prior to final preparation of the polypeptide. The addition of peptide moieties to polypeptides to engender secretion or excretion, to improve stability and to facilitate purification, among others, are familiar and routine techniques in the art. A preferred fusion protein comprises a heterologous region from immunoglobulin that is useful to solubilize proteins. For example, EP-A-O 464 533 (Canadian counterpart 2045869) discloses fusion proteins comprising various portions of constant region of immunoglobulin molecules together with another human protein or part thereof. In many cases, the Fc part in a fusion protein is thoroughly advantageous for fise in therapy and diagnosis and thus results, for example, in improved pharmacokinetic properties (EP-A 0232 262). On the other to hand, for some uses it would be desirable to be able to delete the Fc part after the fusion protein has been expressed, detected and purified in the advantageous manner described. This is the case when Fc portion proves to be a hindrance to use in therapy and diagnosis, for example when the fusion protein is to be used as antigen for immunizations. In drug discovery, for example, human proteins, such as, 15 hIL5-receptor has been fused with Fc portions for the purpose of high-throughput screening assays to identify antagonists of hIL-5. See Bennett, D. et al. (1995) J.

Molec. Recogn. 8:52-58 and Johanson, K. et al. (1995) J. Biol. Chem. 270 (16):9459-9471.

The E. faecalis polypeptides can be recovered and purified from recombinant cell cultures by well-known methods including ammonium sulfate or ethanol precipitation, acid extraction, anion or cation exchange chromatography, phosphocellulose chromatography, hydrophobic interaction chromatography, affinity chromatography, hydroxylapatite chromatography, lectin chromatography and high performance liquid chromatography ("HPLC") is employed for purification.

Polypeptides of the present invention include naturally purified products, products of chemical synthetic procedures, and products produced by recombinant techniques from a prokaryotic or eukaryotic host, including, for example, bacterial, yeast, higher plant, insect and mammalian cells.

Polypeptides and Fragments The invention further provides an isolated E. faecalis polypeptide having an amino acid sequence in Table 1, or a peptide or polypeptide comprising a portion of the above polypeptides.

Variant and Mutant Polypeptides To improve or alter the characteristics of E. faecalis polypeptides of the present invention, protein engineering may be employed. Recombinant DNA technology known to those skilled in the art can be used to create novel mutant o0 proteins or muteins including single or multiple amino acid substitutions, deletions, additions, or fusion proteins. Such modified polypeptides can show, enhanced activity or increased stability. In addition, they may be purified in higher yields and show better solubility than the corresponding natural polypeptide, at least under certain purification and storage conditions.

N-Terminal and C-Terminal Deletion Mutants S. It is known in the art that one or more amino acids may be deleted from the N-terminus or C-terminus without substantial loss of biological function. For instance, Ron et al. J. Biol. Chem., 268:2984-2988 (1993), reported modified KGF 20 proteins that had heparin binding activity even if 3, 8, or 27 N-terminal amino acid residues were missing. Accordingly, the present invention provides polypeptides having one or more residues deleted from the amino terminus of the amino acid sequence of the E. faecalis polypeptides shown in Table 1, and polynucleotides encoding such polypeptides.

25 Similarly, many examples of biologically functional C-terminal deletion muteins are known. For instance, Interferon gamma shows up to ten times higher activities by deleting 8-10 amino acid residues from the carboxy terminus of the protein See, Dobeli, et al. (1988) J. Biotechnology 7:199-216. Accordingly, the present invention provides polypeptides having one or more residues from the carboxy terminus of the amino acid sequence of the E.faecalis polypeptides shown in Table 1. The invention also provides polypeptides having one or more amino acids deleted from both the amino and the carboxyl termini as described below.

The present invention is further directed to polynucleotide encoding portions or fragments of the amino acid sequences described herein as well as to portions or fragments of the isolated amino acid sequences described herein. Fragments include portions of the amino acid sequences of Table 1, are at least 5 contiguous amino acid in length, are selected from any two integers, one of which representing a N-terminal position. The initiation codon of the polypeptides of thd present inventions position 1. Every combination of a N-terminal and C-terminal position that a fragment at least contiguous amino acid residues in length could occupy, on any given amino acid sequence of Table 1 is included in the invention. At least means a fragment may be contiguous amino acid residues in length or any integer between 5 and the number of residues in a full length amino acid sequence minus 1. Therefore, included in the 15 invention are contiguous fragments specified by any N-terminal and C-terminal positions of amino acid sequence set forth in Table 1 wherein the contiguous fragment is any integer between 5 and the number of residues in a full length sequence minus 1.

Further, the invention includes polypeptides comprising fragments specified by size, in amino acid residues, rather than by N-terminal and C-terminal positions.

The invention includes any fragment size, in contiguous amino acid residues, selected from integers between 5 and the number of residues in a full length sequence minus 1.

Preferred sizes of contiguous polypeptide fragments include about 5 amino acid residues, about 10 amino acid residues, about 20 amino acid residues, about 30 amino acid residues, about 40 amino acid residues, about 50 amino acid residues, about 100 amino acid residues, about 200 amino acid residues, about 300 amino acid residues, and about 400 amino acid residues. The preferred sizes are, of course, meant to exemplify, not limit, the present invention as all size fragments representing any integer between 5 and the number of residues in a full length sequence minus 1 are included in the invention. The present invention also provides for the exclusion of any fragments specified by N-terminal and C-terminal positions or by size in amino acid residues as described above. Any number of fragments specified by N-terminal and C-terminal positions or by size in amino acid residues as described above may be excluded.

The above fragments need not be active since they would be useful, for example, in immunoassays, in epitope mapping, epitope tagging, to generate antibodies to a particular portion of the protein, as vaccines, and as molecular weight markers.

Other Mutants In addition to N- and C-terminal deletion forms of the protein discussed above, it also will be recognized by one of ordinary skill in the art that some amino acid sequences of the E. faecalis polypeptide can be varied without significant effect of the structure or function of the protein. If such differences in sequence are contemplated, 15 it should be remembered that there will be critical areas on the protein which Sdetermine activity.

Thus, the invention further includes variations of the E. faecalis polypeptides which show substantial E. faecalis polypeptide activity or which include regions of E.

°faecalis protein such as the protein portions discussed below. Such mutants include deletions, insertions, inversions, repeats, and type substitutions selected according to general rules known in the art so as to have little effect on activity. For example, guidance concerning how to make phenotypically silent amino acid substitutions is provided. There are two main approaches for studying the tolerance of an amino acid sequence to change. See, Bowie, J. U. et al. (1990), Science 247:1306-1310. The first method relies on the process of evolution, in which mutations are either accepted or rejected by natural selection. The second approach uses genetic engineering to introduce amino acid changes at specific positions of a cloned gene and selections or screens to identify sequences that maintain functionality.

These studies have revealed that proteins are surprisingly tolerant of amino acid substitutions. The studies indicate which amino acid changes are likely to be permissive at a certain position of the protein. For example, most buried amino acid residues require nonpolar side chains, whereas few features of surface side chains are generally conserved. Other such phenotypically silent substitutions are described by Bowie et al. (supra) and the references cited therein. Typically seen as conservative substitutions are the replacements, one for another, among the aliphatic amino acids Ala, Val, Leu and Ile; interchange of the hydroxyl residues Ser and Thr, exchange of the acidic residues Asp and Glu, substitution between the amide residues Asn and Gin, exchange of the basic residues Lys and Arg and replacements among the aromatic residues Phe, Tyr.

Thus, the fragment, derivative, analog, or homolog of the polypeptide of Table 1, or that encoded by the plaimds listed in Table 1, may be: one in which one or more of the amino acid residues are substituted with a conserved or non-conserved amino acid residue (preferably a conserved amino acid residue) and such substituted 15 amino acid residue may or may not be one encoded by the genetic code: or (ii) one in which one or more of the amino acid residues includes a substituent group: or (iii) one in which the E. faecalis polypeptide is fused with another compound, such as a compound to increase the half-life of the polypeptide (for example, polyethylene glycol): or (iv) one in which the additional amino acids are fused to the above form of 9 the polypeptide, such as an IgG Fc fusion region peptide or leader or secretory sequence or a sequence which is employed for purification of the above form of the polypeptide or a proprotein sequence. Such fragments, derivatives and analogs are deemed to be within the scope of those skilled in the art from the teachings herein.

Thus, the E. faecalis polypeptides of the present invention may include one or more amino acid substitutions, deletions, or additions, either from natural mutations or human manipulation. As indicated, changes are preferably of a minor nature, such as conservative amino acid substitutions that do not significantly affect the folding or activity of the protein (see Table 3).

Amino acids in the E. faecalis proteins of the present invention that are essential for function can be identified by methods known in the art, such as sitedirected mutagenesis or alanine-scanning mutagenesis. See, Cunningham et al.

(1989) Science 244:1081-1085. The latter procedure introduces single alanine mutations at every residue in the molecule. The resulting mutant molecules are then tested for biological activity using assays appropriate for measuring the finction of the particular protein.

Of special interest are substitutions of charged amino acids with other charged or neutral amino acids which may produce proteins with highly desirable improved characteristics, such as less aggregation. Aggregation may not only reduce activity but also be problematic when preparing pharmaceutical formulations, because aggregates can be immunogenic. See, Pinckard et al., (1967) Clin. Exp. Immunol. 2:331-340; Robbins, et al., (1987) Diabetes 36:838-845; Cleland, et al., (1993) Crit. Rev.

Therapeutic Drug Carrier Systems 10:307-377.

The polypeptides of the present invention are preferably provided in an isolated form, and preferably are substantially purified. A recombinantly produced version of the E. faecalis polypeptide can be substantially purified by the one-step method described by Smith et al. (1988) Gene 67:31-40. Polypeptides of the invention also can be purified from natural or recombinant sources using antibodies .,*directed against the polypeptides of the invention in methods which are well known in the art of protein purification.

°oo ~The invention further provides for isolated E. faecalis polypeptides comprising an amino acid sequence selected from the group consisting of: the amino acid sequence of a full-length E. faecalis polypeptide having the complete amino acid sequence shown in Table 1; the amino acid sequence of a full-length E.

faecalis polypeptide having the complete amino acid sequence shown in Table I excepting the N-terminal methionine; the complete amino acid sequence encoded by the plaimds listed in Table 1; and the complete amino acid sequence excepting the N-terminal methionine encoded by the plaimds listed in Table 1. The polypeptides of the present invention also include polypeptides having an amino acid sequence at least 80% identical, more preferably at least 90% identical, and still more preferably 95%, 96%, 97%, 98% or 99% identical to those described in and above.

Further polypeptides of the present invention include polypeptides which have at least 90% similarity, more preferably at least 95% similarity, and still more preferably at least 96%, 97%, 98% or 99% similarity to those described above.

A further embodiment of the invention relates to a polypeptide which comprises the amino acid sequence of a E. faecalis polypeptide having an amino acid sequence which contains at least one conservative amino acid substitution, but not more than 50 conservative amino acid substitutions, not more than 40 conservative amino acid substitutions, not more than 30 conservative amino acid substitutions, and not more than 20 conservative amino acid substitutions. Also provided are polypeptides which comprise the amino acid sequence of a E. faecalis polypeptide, having at least one, but not more than 10, 9, 8, 7, 6, 5, 4, 3, 2 or 1 conservative amino acid substitutions.

By a polypeptide having an amino acid sequence at least, for example, "identical" to a query amino acid sequence of the present invention, it is intended that the amino acid sequence of the subject polypeptide is identical to the query sequence except that the subject polypeptide sequence may include up to five amino acid alterations per each 100 amino acids of the query amino acid sequence. In other words, to obtain a polypeptide having an amino acid sequence at least 95% identical to a query amino acid sequence, up to 5% of the amino acid residues in the subject sequence may be inserted, deleted, (indels) or substituted with another amino acid.

These alterations of the reference sequence may occur at the amino or carboxy terminal positions of the reference amino acid sequence or anywhere between those terminal positions, interspersed either individually among residues in the reference sequence or in one or more contiguous groups within the reference sequence.

As a practical matter, whether any particular polypeptide is at least 96%, 97%, 98% or 99% identical to, for instance, the amino acid sequences shown in Table 1 or to the amino acid sequence encoded by the plaimds listed in Table I can be detennined conventionally using known computer programs. A preferred method for determining the best overall match between a query sequence (a sequence of the present invention) and a subject sequence, also referred to as a global sequence alignment, can be determined using the FASTDB computer program based on the algorithm of Brutlag et al., (1990) Comp. App. Biosci. 6:237-245. In a sequence alignment the query and subject sequences are both amino acid sequences. The result of said global sequence alignment is in percent identity. Preferred parameters used in a FASTDB amino acid alignment are: Matrix=PAM 0, k-tuple=2, Mismatch to Penalty=l, Joining Penalty=20, Randomization Group Length=0, Cutoff Score=l, Window Size=sequence length, Gap Penalty=5, Gap Size Penalty=0.05, Window Size=500 or the length of the subject amino acid sequence, whichever is shorter.

If the subject sequence is shorter than the query sequence due to N- or Cterminal deletions, not because of internal deletions, the results, in percent identity, 15 must be manually corrected. This is because the FASTDB program does not account for N- and C-terminal truncations of the subject sequence when calculating global percent identity. For subject sequences truncated at the N- and C-termini, relative to the query sequence, the percent identity is corrected by calculating the number of residues of the query sequence that are N- and C-terminal of the subject sequence, which are not matched/aligned with a corresponding subject residue, as a percent of the total bases of the query sequence. Whether a residue is matched/aligned is determined by results of the FASTDB sequence alignment. This percentage is then subtracted from the percent identity, calculated by the above FASTDB program using the specified parameters, to arrive at a final percent identity score. This final percent identity score is what is used for the purposes of the present invention. Only residues to the N- and C-termini of the subject sequence, which are not matched/aligned with the query sequence, are considered for the purposes of manually adjusting the percent identity score. That is, only query amino acid residues outside the farthest N- and C-terminal residues of the subject sequence.

For example, a 90 amino acid residue subject sequence is aligned with a 100 residue query sequence to determine percent identity. The deletion occurs at the Nterminus of the subject sequence and therefore, the FASTDB alignment does not match/align with the first 10 residues at the N-terminus. The 10 unpaired residues represent 10% of the sequence (number of residues at the N- and C- termini not matched/total number of residues in the query sequence) so 10% is subtracted from the percent identity score calculated by the FASTDB program. If the remaining residues were perfectly matched the final percent identity would be 90%. In another example, a 90 residue subject sequence is compared with a 100 residue query sequence. This time the deletions are internal so there are no residues at the N- or Ctermini of the subject sequence which are not matched/aligned with the query. In this case the percent identity calculated by FASTDB is not manually corrected. Once again, only residue positions outside the N- and C-terminal ends of the subject sequence, as displayed in the FASTDB alignment, which are not matched/aligned 15 with the query sequence are manually corrected. No other manual corrections are to made for the purposes of the present invention.

The above polypeptide sequences are included irrespective of whether they have their normal biological activity. This is because even where a particular polypeptide molecule does not have biological activity, one of skill in the art would 20 still know how to use the polypeptide, for instance, as a vaccine or to generate antibodies. Other uses of the polypeptides of the present invention that do not have E. faecalis activity include, inter alia, as epitope tags, in epitope mapping, and as molecular weight markers on SDS-PAGE gels or on molecular sieve gel filtration columns using methods known to those of skill in the art.

25 As described below, the polypeptides of the present invention can also be used to raise polyclonal and monoclonal antibodies, which are useful in assays for detecting E. faecalis protein expression or as agonists and antagonists capable of enhancing or inhibiting E. faecalis protein function. Further, such polypeptides can be used in the yeast two-hybrid system to "capture" E. faecalis protein binding proteins which are also candidate agonists and antagonists according to the present invention.

See, Fields et al. (1989) Nature 340:245-246.

Epilope-Bearing Portions In another aspect, the invention provides peptides and polypeptides comprising epitope-bearing portions of the E. faecalis polypeptides of the present invention. These epitopes are immunogenic or antigenic epitopes of the polypeptides of the present invention. An "immunogenic epitope" is defined as a part of a protein that elicits an antibody response when the whole protein or polypeptide is the immunogen. These immunogenic epitopes are believed to be confined to a few loci on the molecule. On the other hand, a region of a protein molecule to which an antibody can bind is defined as an "antigenic determinant" or "antigenic epitope." The number of immunogenic epitopes of a protein generally is less than the number of antigenic epitopes. See, Geysen, et al. (1983) Proc. Natl. Acad. Sci. USA 81:3998- 4002.

t15 Predicted antigenic epitopes are shown in Table 4, below. It is pointed out that Table 4 only lists amino acid residues comprising epitopes predicted to have the highest degree of antigenicity. The polypeptides not listed in Table 4 and portions of polypeptides not listed in Table 4 are not considered non-antigenic. This is because they may still be antigenic in vivo but merely not recognized as such by the particular algorithm used. Thus, Table 4 lists the amino acid residues comprising preferred antigenic epitopes but not a complete list. Amino acid residues comprising other anigenic epitopes may be determined by algorithms similar to the Jameson-Wolf analysis or by in vivo testing for an antigenic response using the methods described herein or those known in the art.

As to the selection of peptides or polypeptides bearing an antigenic epitope that contain a region of a protein molecule to which an antibody can bind), it is well known in that art that relatively short synthetic peptides that mimic part of a protein sequence are routinely capable of eliciting an antiserum that reacts with the partially mimicked protein. See, Sutcliffe, et al., (1983) Science 219:660-666.

Peptides capable of eliciting protein-reactive sera are frequently represented in the primary sequence of a protein, can be characterized by a set of simple chemical rules, and are confined neither to immunodominant regions of intact proteins immunogenic epitopes) nor to the amino or carboxyl terminals. Peptides that are extremely hydrophobic and those of six or fewer residues generally are ineffective at inducing antibodies that bind to the mimicked protein; longer, peptides, especially those containing proline residues, usually are effective. See, Sutcliffe, et al., supra, p.

661. For instance, 18 of 20 peptides desig ed according to these guidelines, containing 8-39 residues covering 75% of the sequence of the influenza virus hemagglutinin HAl polypeptide chain, induced antibodies that reacted with the HAl protein or intact virus; and 12/12 peptides from the MuLV polymerase and 18/18 from the rabies glycoprotein induced antibodies that precipitated the respective proteins.

Antigenic epitope-bearing peptides and polypeptides of the invention are therefore useful to raise antibodies, including monoclonal antibodies, that bind 15 specifically to a polypeptide of the invention. Thus, a high proportion of hybridomas obtained by fusion of spleen cells from donors immunized with an antigen epitope-bearing peptide generally secrete antibody reactive with the native protein.

See Sutcliffe, et al., supra, p. 663. The antibodies raised by antigenic epitope-bearing peptides or polypeptides are useful to detect the mimicked protein, and antibodies to different peptides may be used for tracking the fate of various regions of a protein precursor which undergoes post-translational processing. The peptides and *oo anti-peptide antibodies may be used in a variety of qualitative or quantitative assays for the mimicked protein, for instance in competition assays since it has been shown that even short peptides about 9 amino acids) can bind and displace the larger 25 peptides in immunoprecipitation assays. See, Wilson, et al., (1984) Cell 37:767-778. The anti-peptide antibodies of the invention also are useful for purification of the mimicked protein, for instance, by adsorption chromatography using methods known in the art.

Antigenic epitope-bearing peptides and polypeptides of the invention designed according to the above guidelines preferably contain a sequence of at least seven, more preferably at least nine and most preferably between about 10 to about amino acids any integer between 7 and 50) contained within the amino acid sequence of a polypeptide of the invention. However, peptides or polypeptides comprising a larger portion of an amino acid sequence of a polypeptide of the invention, containing about 50 to about 100 amino acids, or any length up to and including the entire amino acid sequence of a polypeptide of the invention, also are considered epitope-bearing peptides or polypeptides of the invention and also are useful for inducing antibodies that react with the mimicked protein. Preferably, the amino acid sequence of the epitope-bearing peptide is selected to provide substantial solubility in aqueous solvents the sequence includes relatively hydrophilic residues and highly hydrophobic sequences are preferably avoided); and sequences containing proline residues are particularly preferred.

Non-limiting examples of antigenic polypeptides or peptides that can be used 15 to generate an enterococcal-specific immune response or antibodies include portions of the amino acid sequences identified in Table 1. More specifically, Table 4 discloses a list of non-limiting residues that are involved in the antigenicity of the epitope-bearing fragments of the present invention. Therefore, the present inventions provides for isolatd and purified antigenic epitope-bearing fragements of the polypeptides of the present invention comprising a peptide sequences of Table 4. The antigenic epitopebearing fragments comprising a peptide sequence of Table 4 preferably contain a sequence of at least seven, more preferably at least nine and most preferably between about 10 to about 50 amino acids any integer between 7 and 50) of a polypeptide oo of the present invention. That is, included in the present invention are antigenic polypeptides between the integers of 7 and 50 amino acid in length comprising one or more of the sequences of Table 4. Therefore, in most cases, the polypeptides of Table 4 make up only a portion of the antigenic polypeptide. All combinations of sequences between the integers of 7 and 50 amino acid in length comprising one or more of the sequences of Table 4 are included. The antigenic epitope-bearing fragements may be specified by either the number of contiguous amino acid residues or by specific N-tenninal and C-terminal positions as described above for the polypeptide fragements of the present invention, wherein the initiation codon is residue 1. Any number of the described antigenic epitope-bearing fragements of the present invention may also be excluded from the present invention in the same manner.

The epitope-bearing peptides and polypeptides of the invention may be produced by any conventional means for making peptides or polypeptides including recombinant means using nucleic acid molecules of the invention. For instance, an epitope-bearing amino acid sequence of the present invention may be fused to a larger polypeptide which acts as a carrier during recombinant production and purification, as well as during immunization to produce anti-peptide antibodies. Epitope-bearing peptides also may be synthesized using known methods of chemical synthesis. For instance, Houghten has described a simple method for synthesis of large numbers of peptides, such as 10-20 mg of 248 different 13 residue peptides representing single amino acid variants of a segment of the HA1 polypeptide which were prepared and characterized (by ELISA-type binding studies) in less than four weeks (Houghten, R.

A. Proc. Natl. Acad. Sci. USA 82:5131-5135 (1985)). This "Simultaneous Multiple •Peptide Synthesis (SMPS)" process is further described in U.S. Patent No. 4,631,211 to.Houghten and coworkers (1986). In this procedure the individual resins for the solid-phase synthesis of various peptides are contained in separate solvent-permeable packets, enabling the optimal use of the many identical repetitive steps involved in solid-phase methods. A completely manual procedure allows 500-1000 or more syntheses to be conducted simultaneously (Houghten et al. (1985) Proc. Natl. Acad.

Sci. 82:5131-5135 at 5134.

Epitope-bearing peptides and polypeptides of the invention are used to induce antibodies according to methods well known in the art. See, Sutcliffe, et al., supra;; Wilson, et al., supra;; and Bittle, et al. (1985) J. Gen. Virol. 66:2347-2354.

Generally, animals may be immunized with free peptide; however, anti-peptide antibody titer may be boosted by coupling of the peptide to a macromolecular carrier, such as keyhole limpet hemacyanin (KLH) or tetanus toxoid. For instance, peptides containing cysteine may be coupled to carrier using a linker such as m-maleimidobenzoyl-N-hydroxysuccinimide ester (MBS), while other peptides may be coupled to carrier using a more general linking agent such as glutaraldehyde.

Animals such as rabbits, rats and mice are immunized with either free or carrier-coupled peptides, for instance, by intraperitoneal and/or intradermal injection of emulsions containing about 100 ig peptide or carrier protein and Freund's adjuvant.

Several booster injections may be needed, for instance, at intervals of about two weeks, to provide a useful titer of anti-peptide antibody which can be detected, for example, by ELISA assay using free peptide adsorbed to a solid surface. The titer of anti-peptide antibodies in serum from an immunized animal may be increased by selection of anti-peptide antibodies, for instance, by adsorption to the peptide on a solid support and elution of the selected antibodies according to methods well known 15 in the art.

Sn. Immunogenic epitope-bearing peptides of the invention, those parts of a protein that elicit an antibody response when the whole protein is the immunogen, are identified according to methods known in the art. For instance, Geysen, el al., supra, S• discloses a procedure for rapid concurrent synthesis on solid supports of hundreds of peptides of sufficient purity to react in an ELISA. Interaction of synthesized peptides with antibodies is then easily detected without removing them from the support. In this manner a peptide bearing an immunogenic epitope of a desired protein may be identified routinely by one of ordinary skill in the art. For instance, the immunologically important epitope in the coat protein of foot-and-mouth disease virus was located by Geysen et al. supra with a resolution of seven amino acids by synthesis of an overlapping set of all 208 possible hexapeptides covering the entire 213 amino acid sequence of the protein. Then, a complete replacement set of peptides in which all 20 amino acids were substituted in turn at every position within the epitope were synthesized, and the particular amino acids conferring specificity for the reaction with antibody were determined. Thus, peptide analogs of the epitope-bearing peptides of the invention can be made routinely by this method. U.S. Patent No.

4,708,781 to Geysen (1987) further describes this method of identifying a peptide bearing an immnunogenic epitope of a desired protein.

Further still, U.S. Patent No. 5,194,392, to Geysen (1990), describes a general method of detecting or determining the sequence of monomers (amino acids or other compounds) which is a topological equivalent of the epitope a "mimotope") which is complementary to a particular paratope (antigen binding site) of an antibody of interest. More generally, U.S. Patent No. 4,433,092, also to Geysen (1989), describes a method of detecting or determining a sequence of monomers which is a topographical equivalent of a ligand which is complementary to the ligand binding site of a particular receptor of interest. Similarly, U.S. Patent No. 5,480,971 to Houghten, R. A. et al. (1996) discloses linear Ci-C 7 -alkyl peralkylated oligopeptides and sets and ,libraries of such peptides, as well as methods for using such oligopeptide sets and 15 libraries for determining the sequence of a peralkylated oligopeptide that preferentially binds to an acceptor molecule of interest. Thus, non-peptide analogs of the epitope-bearing peptides of the invention also can be made routinely by these methods. The entire disclosure of each document cited in this section on "Polypeptides and Fragments" is hereby incorporated herein by reference.

20 As one of skill in the art will appreciate, the polypeptides of the present invention and the epitope-bearing fragments thereof described above can be combined with parts of the constant domain of immunoglobulins (IgG), resulting in chimeric polypeptides. These fusion proteins facilitate purification and show an increased half-life in vivo. This has been shown, for chimeric proteins consisting of the first two domains of the human CD4-polypeptide and various domains of the constant regions of the heavy or light chains of mammalian immunoglobulins. (EPA 0,394,827; Traunecker et al. (1988) Nature 331:84-86. Fusion proteins that have a disulfide-linked dimeric structure due to the IgG part can also be more efficient in binding and neutralizing other molecules than a monomeric E. faecalis polypeptide or fragment thereof alone. See Fountoulakis et al. (1995) J. Biochem. 270:3958-3964.

Nucleic acids encoding the above epitopes of E. faecalis polypeptides can also be recombined with a gene of interest as an epitope tag to aid in detection and purification of the expressed polypeptide.

Antibodies E. faecalis protein-specific antibodies for use in the present invention can be raised against the intact E. faecalis protein or an antigenic polypeptide fragment thereof, which may be presented together with a carrier protein, such as an albumin, to an animal system (such as rabbit or mouse) or, if it is long enough (at least about amino acids), without a carrier.

As used herein, the term "antibody" (Ab) or "monoclonal antibody" (Mab) is meant to include intact molecules, single chain whole antibodies, and antibody fragments. Antibody fragments of the present invention include Fab and F(ab')2 and 15 other fragments including single-chain Fvs (scFv) and disulfide-linked Fvs (sdFv).

Also included in the present invention are chimeric and humanized monoclonal antibodies and polyclonal antibodies specific for the polypeptides of the present invention. The antibodies of the present invention may be prepared by any of a variety of methods. For example, cells expressing a polypeptide of the present 20 invention or an antigenic fragment thereof can be administered to an animal in order to induce the production of sera containing polyclonal antibodies. For example, a preparation of E. faecalis polypeptide or fragment thereof is prepared and purified to render it substantially free of natural contaminants. Such a preparation is then introduced into an animal in order to produce polyclonal antisera of greater specific 25 activity.

In a preferred method, the antibodies of the present invention are monoclonal antibodies or binding fragments thereof. Such monoclonal antibodies can be prepared using hybridoma technology. See, Harlow et al., ANTIBODIES: A LABORATORY MANUAL, (Cold Spring Harbor Laboratory Press, 2nd ed. 1988); Hammerling, et al., in: MONOCLONAL ANTIBODIES AND T-CELL HYBRIDOMAS 563-681 (Elsevier, 1981). Fab and F(ab')2 fragments may be produced by proteolytic cleavage, using enzymes such as papain (to produce Fab fragments) or pepsin (to produce F(ab')2 fragments). Alternatively, E. faecalis polypeptide-binding fragments, chimeric, and humanized antibodies can be produced through the application of recombinant DNA technology or through synthetic chemistry using methods known in the art.

Alternatively, additional antibodies capable of binding to the polypeptide antigen of the present invention may be produced in a two-step procedure through the use of anti-idiotypic antibodies. Such a method makes use of the fact that antibodies are themselves antigens, and that, therefore, it is possible to obtain an antibody which binds to a second antibody. In accordance with this method, E. faecalis polypeptide-specific antibodies are used to immunize an animal, preferably a mouse.

The splenocytes of such an animal are then used to produce hybridoma cells, and the 15 hybridoma cells are screened to identify clones which produce an antibody whose ability to bind to the E. faecalis polypeptide-specific antibody can be blocked by the E. faecalis polypeptide antigen. Such antibodies comprise anti-idiotypic antibodies to the E. faecalis polypeptide-specific antibody and can be used to immunize an animal to induce formation of further E. faecalis polypeptide-specific antibodies.

Antibodies and fragements thereof of the present invention may be described by the portion of a polypeptide of the present invention recognized or specifically bound by the antibody. Antibody binding fragements of a polypeptide of the present invention may be described or specified in the same manner as for polypeptide fragements discussed above., i.e, by N-terminal and C-terminal positions or by size in contiguous amino acid residues. Any number of antibody binding fragments, of a polypeptide of the present invention, specified by N-terminal and C-terminal positions or by size in amino acid residues, as described above, may also be excluded from the present invention. Therefore, the present invention includes antibodies the specifically bind a particuarlly discribed fragement of a polypeptide of the present invention and allows for the exclusion of the same.

Antibodies and fragements thereof of the present invention may also be described or specified in terms of their cross-reactivity. Antibodies and fragements that do not bind polypeptides of any other species of Enterococcus other than E.

faecalis are included in the present invention. Likewise, antibodies and fragements that bind only species of Enterococcus, i.e. antibodies and fragements that do not bind bacteria from any genus other than Enterococcus, are included in the present invention.

Diagnostic Assays The present invention further relates to methods for assaying staphylococcal infection in an animal by detecting the expression of genes encoding staphylococcal polypeptides of the present invention. The methods comprise analyzing tissue or body fluid from the animal for Enterococcus-specific antibodies, nucleic acids, or 15 proteins. Analysis of nucleic acid specific to Enterococcus is assayed by PCR or hybridization techniques using nucleic acid sequences of the present invention as either hybridization probes or primers. See, Sambrook et al. Molecular cloning: A Laboratory Manual (Cold Spring Harbor Laboratory Press, 2nd ed., 1989, page 54 reference); Eremeeva et al. (1994) J. Clin. Microbiol. 32:803-810 (describing differentiation among spotted fever group Rickettsiae species by analysis of restriction fragment length polymorphism of PCR-amplified DNA) and Chen et al. 1994 J. Clin.

Microbiol. 32:589-595 (detecting B. burgdorferi nucleic acids via PCR).

Where diagnosis of a disease state related to infection with Enterococcus has already been made, the present invention is useful for monitoring progression or 25 regression of the disease state whereby patients exhibiting enhanced Enterococcus gene expression will experience a worse clinical outcome relative to patients expressing these gene(s) at a lower level.

By "biological sample" is intended any biological sample obtained from an animal, cell line, tissue culture, or other source which contains Enterococcus polypeptide, mRNA, or DNA. Biological samples include body fluids (such as saliva, blood, plasma, urine, mucus, synovial fluid, etc.) tissues (such as muscle, skin, and cartilage) and any other biological source suspected of containing Enterococcus polypeptides or nucleic acids. Methods for obtaining biological samples such as tissue are well known in the art.

The present invention is useful for detecting diseases related to Enterococcus infections in animals. Preferred animals include monkeys, apes, cats, dogs, birds, cows, pigs, mice, horses, rabbits and humans. Particularly preferred are humans.

Total RNA can be isolated from a biological sample using any suitable technique such as the single-step guanidinium-thiocyanate-phenol-chloroform method described in Chomczynski et al. (1987) Anal. Biochem. 162:156-159. mRNA encoding Enterococcus polypeptides having sufficient homology to the nucleic acid sequences identified in Table 1 to allow for hybridization between complementary sequences are then assayed using any appropriate method. These include Northern blot analysis, S 1 15 nuclease mapping, the polymerase chain reaction (PCR), reverse transcription in combination with the polymerase chain reaction (RT-PCR), and reverse transcription in combination with the ligase chain reaction (RT-LCR).

Northern blot analysis can be performed as described in Harada et al. (1990) Cell 63:303-312. Briefly, total RNA is prepared from a biological sample as described above. For the Northern blot, the RNA is denatured in an appropriate buffer (such as glyoxal/dimethyl sulfoxide/sodium phosphate buffer), subjected to agarose gel electrophoresis, and transferred onto a nitrocellulose filter. After the RNAs have been linked to the filter by a UV linker, the filter is prehybridized in a solution containing formamide, SSC, Denhardt's solution, denatured salmon sperm, SDS, and sodium 25 phosphate buffer. A E. faecalis polynucleotide sequence shown in Table 1 labeled according to any appropriate method (such as the 32 P-multiprimed DNA labeling system (Amersham)) is used as probe. After hybridization overnight, the filter is washed and exposed to x-ray film. DNA for use as probe according to the present invention is described in the sections above and will preferably at least 15 nucleotides in length.

S1 mapping can be performed as described in Fujita et al. (1987) Cell 49:357-367. To prepare probe DNA for use in Sl mapping, the sense strand of an above-described E.faecalis DNA sequence of the present invention is used as a template to synthesize labeled antisense DNA. The antisense DNA can then be digested using an appropriate restriction endonuclease to generate further DNA probes of a desired length. Such antisense probes are useful for visualizing protected bands corresponding to the target mRNA mRNA encoding Enlerococcus polypeptides).

Levels of mRNA encoding Enterococcus polypeptides arc assayed, for e.g., using the RT-PCR method described in Makino ct al. (1990) Technique 2:295-301.

By this method, the radioactivities of the "amplicons" in the polyacrylamide gel bands are linearly related to the initial concentration of the target mRNA. Briefly, this method involves adding total RNA isolated from a biological sample in a reaction 15 mixture containing a RT primer and appropriate buffer. After incubating for primer annealing, the mixture can be supplemented with a RT buffer, dNTPs, DTT, RNase inhibitor and reverse transcriptase. After incubation to achieve reverse transcription of the RNA, the RT products are then subject to PCR using labeled primers.

Alternatively, rather than labeling the primers, a labeled dNTP can be included in the 20 PCR reaction mixture. PCR amplification can be performed in a DNA thermal cycler according to conventional techniques. After a suitable number of rounds to achieve amplification, the PCR reaction mixture is electrophoresed on a polyacrylamide gel.

After drying the gel, the radioactivity of the appropriate bands (corresponding to the mRNA encoding the Enterococcus polypeptides of the present invention) are 25 quantified using an imaging analyzer. RT and PCR reaction ingredients and conditions, reagent and gel concentrations, and labeling methods are well known in the art. Variations on the RT-PCR method will be apparent to the skilled artisan. Other PCR methods that can detect the nucleic acid of the present invention can be found in PCR PRIMER: A LABORATORY MANUAL Dieffenbach et al. eds., Cold Spring Harbor Lab Press, 1995).

The polynucleotides of the present invention, including both DNA and RNA, may be used to detect polynucleotides of the present invention or Enterococcal species including E. faecalis using bio chip technology. The present invention includes both high density chip arrays (>1000 oligonucleotides per cm 2 and low density chip arrays (<1000 oligonucleotides per cm 2 Bio chips comprising arrays of polynucleotides of the present invention may be used to detect Enterococcal species, including E. faecalis, in biological and environmental samples and to diagnose an animal, including humans, with an E. faecalis or other Enterococcal infection. The bio chips of the present invention may comprise polynucleotide sequences of other pathogens including bacteria, viral, parasitic, and fungal polynucleotide sequences, in addition to the polynucleotide sequences of the present invention, for use in rapid diffenertial pathogenic detection and diagnosis. The bio chips can also be used to monitor an E. faecalis or other Enterococcal infections and to monitor the genetic 15 changes (deletions, insertions, mismatches, etc.) in response to drug therapy in the clinic and drug development in the laboratory. The bio chip technology comprising .arrays of polynucleotides of the present invention may also be used to simultaneously monitor the expression of a multiplicity of genes, including those of the present invention. The polynucleotides used to comprise a selected array may be specified in the same manner as for the fragements, i.e, by their 5' and 3' positions or length in contigious base pairs and include from. Methods and particular uses of the polynucleotides of the present invention to detect Enterococcal species, including E.

faecalis, using bio chip technology include those known in the art and those of: U.S.

Patent Nos. 5510270, 5545531, 5445934, 5677195, 5532128, 5556752, 5527681, 25 5451683, 5424186, 5607646, 5658732 and World Patent Nos. WO/9710365, WO/9511995, WO/9743447, WO/9535505, each incorporated herein in their entireties.

Biosensors using the polynucleotides of the present invention may also be used to detect, diagnose, and monitor E. faecalis or other Enterococcal species and infections thereof. Biosensors using the polynucleotides of the present invention may also be used to detect particular polynucleotides of the present invention. Biosensors using the polynucleotides of the present invention may also be used to monitor the genetic changes (deletions, insertions, mismatches, etc.) in response to drug therapy in the clinic and drug development in the laboratory. Methods and particular uses of the polynucleotides of the present invention to detect Enterococcal species, including E.

faecalis, using biosenors include those known in the art and those of: U.S. Patent Nos 5721102, 5658732, 5631170, and World Patent Nos. W097/35011, WO/9720203, each incorporated herein in their entireties.

Thus, the present invention includes both bio chips and biosensors comprising polynucleotides of the present invention and methods of their use.

Assaying Enterococcus polypeptide levels in a biological sample can occur using any art-known method, such as antibody-based techniques. For example, Enterococcus polypeptide expression in tissues can be studied with classical 15 immunohistological methods. In these, the specific recognition is provided by the 9. primary antibody (polyclonal or monoclonal) but the secondary detection system can utilize fluorescent, enzyme, or other conjugated secondary antibodies. As a result, an immunohistological staining of tissue section for pathological examination is obtained.

Tissues can also be extracted, with urea and neutral detergent, for the liberation of Enterococcus polypeptides for Western-blot or dot/slot assay. See, Jalkanen, M.

et al. (1985) J. Cell. Biol. 101:976-985; Jalkanen, M. et al. (1987) J. Cell Biol.

9* 105:3087-3096. In this technique, which is based on the use of cationic solid phases, quantitation of a Enterococcus polypeptide can be accomplished using an isolated Enterococcus polypeptide as a standard. This technique can also be applied to body S" 25 fluids.

Other antibody-based methods useful for detecting Enterococcus polypeptide gene expression include immunoassays, such as the ELISA and the radioimmunoassay (RIA). For example, a Enterococcus polypeptide-specific monoclonal antibodies can be used both as an immunoabsorbent and as an enzyme-labeled probe to detect and quantify a Enterococcus polypeptide. The amount of a Enterococcus polypeptide present in the sample can be calculated by reference to the amount present in a standard preparation using a linear regression computer algorithm. Such an ELISA is described in lacobelli et al. (1988) Breast Cancer Research and Treatment 11:19-30. In another ELISA assay, two distinct specific monoclonal antibodies can be used to detect Enterococcus polypeptides in a body fluid. In this assay, one of the antibodies is used as the immunoabsorbent and the other as the enzyme-labeled probe.

The above techniques may be conducted essentially as a "one-step" or "two-step" assay. The "one-step" assay involves contacting the Enterococcus So polypeptide with irmnobilized antibody and, without washing, contacting the mixture with the labeled antibody. The "two-step" assay involves washing before contacting the mixture with the labeled antibody. Other conventional methods may also be employed as suitable. It is usually desirable to immobilize one component of the a assay system on a support, thereby allowing other components of the system to be 15 brought into contact with the component and readily removed from the sample.

Variations of the above and other immunological methods included in the present invention can also be found in Harlow et al., ANTIBODIES: A LABORATORY MANUAL, (Cold Spring Harbor Laboratory Press, 2nd.ed. 1988).

Suitable enzyme labels include, for example, those from the oxidase group, 20 which catalyze the production of hydrogen peroxide by reacting with substrate.

Glucose oxidase is particularly preferred as it has good stability and its substrate (glucose) is readily available. Activity of an oxidase label may be assayed by measuring the concentration of hydrogen peroxide formed by the enzyme-labeled antibody/substrate reaction. Besides enzymes, other suitable labels include 25 radioisotopes, such as iodine (1251, 21), carbon 4 sulphur tritium indium and technetium (99Tc), and fluorescent labels, such as fluorescein and rhodamine, and biotin.

Further suitable labels for the Enterococcus polypeptide-specific antibodies of the present invention are provided below. Examples of suitable enzyme labels include malate dehydrogenase, Enterococcal nuclease, delta-5-steroid isomerase, yeast-alcohol dehydrogenase, alpha-glycerol phosphate dehydrogenase, triose phosphate isomerase, peroxidase, alkaline phosphatase, asparaginase, glucose oxidase, beta-galactosidase, ribonuclease, urease, catalase, glucose-6-phosphate dehydrogenase, glucoamylase, and acetylcholine esterase.

Examples of suitable radioisotopic labels include 3 H, 11 n, 251, 131, 32 3 5

S,

14C, SICr, 57 To, 58 Co, 59 Fe, 75 Se, 52 Eu, 9 6 7 Cu, 2 17 Ci, 21 'At, 212 Pb, 47 Sc, etc.

"'In is a preferred isotope where in vivo imaging is used since its avoids the problem of dehalogenation of the 1251 or 31 1-labeled monoclonal antibody by the liver. In addition, this radionucleotide has a more favorable gamma emission energy for imaging.

See, Perkins etal. (1985) Eur. J. Nucl. Med. 10:296-301; Carasquillo et al.

(1987) J. Nucl. Med. 28:281-287. For example,'"In coupled to monoclonal antibodies with I-(P-isothiocyanatobenzyl)-DPTA has shown little uptake in non-tumors tissues, particularly the liver, and therefore enhances specificity of tumor 15 localization. See, Esteban et al. (1987) J. Nucl. Med. 28:861-870.

Examples of suitable non-radioactive isotopic labels include '57Gd, '6Dy, 52 Tr, and 56 Fe.

Examples of suitable fluorescent labels include an 5 2 Eu label, a fluorescein label, an isothiocyanate label, a rhodamine label, a phycoerythrin label, a phycocyanin label, an allophycocyanin label, an o-phthaldehyde label, and a fluorescamine label.

Examples of suitable toxin labels include, Pseudomonas toxin, diphtheria toxin, ricin, and cholera toxin.

Examples of chemiluminescent labels include a luminal label, an isoluminal label, an aromatic acridinium ester label, an imidazole label, an acridinium salt label, an oxalate ester label, a luciferin label, a luciferase label, and an aequorin label.

Examples of nuclear magnetic resonance contrasting agents include heavy metal nuclei such as Gd, Mn, and iron.

Typical techniques for binding the above-described labels to antibodies are provided by Kennedy et al. (1976) Clin. Chim. Acta 70:1-31, and Schurs et al. (1977) Clin. Chim. Acta 81:1-40. Coupling techniques mentioned in the latter are the glutaraldehyde method, the periodate method, the dimaleimide method, the m-maleimidobenzyl-N-hydroxy-succinimide ester method, all of which methods are incorporated by reference herein.

In a related aspect, the invention includes a diagnostic kit for use in screening serum containing antibodies specific against E. faecalis infection. Such a kit may include an isolated E. faecalis antigen comprising an epitope which is specifically immunoreactive with at least one anti-E.faecalis antibody. Such a kit also includes means for detecting the binding of said antibody to the antigen. In specific embodiments, the kit may include a recombinantly produced or chemically synthesized peptide or polypeptide antigen. The peptide or polypeptide antigen may be attached to a solid support.

In a more specific embodiment, the detecting means of the above-described kit includes a solid support to which said peptide or polypeptide antigen is attached.

Such a kit may also include a non-attached reporter-labeled anti-human antibody. In this embodiment, binding of the antibody to the E. faecalis antigen can be detected by binding of the reporter labeled antibody to the anti-E. faecalis polypeptide antibody.

In a related aspect, the invention includes a method of detecting E. faecalis infection in a subject. This detection method includes reacting a body fluid, preferably serum, from the subject with an isolated E.faecalis antigen, and examining the antigen for the presence of bound antibody. In a specific embodiment, the method includes a polypeptide antigen attached to a solid support, and serum is reacted with the support. Subsequently, the support is reacted with a reporter-labeled anti-human antibody. The support is then examined for the presence of reporter-labeled antibody.

The solid surface reagent employed in the above assays and kits is prepared by known techniques for attaching protein material to solid support material, such as polymeric beads, dip sticks, 96-well plates or filter material. These attachment methods generally include non-specific adsorption of the protein to the support or covalent attachment of the protein typically through a free amine group, to a chemically reactive group on the solid support, such as an activated carboxyl, hydroxyl, or aldehyde group. Alternatively, streptavidin coaled plates can be used in conjunction with biotinylated antigen(s).

The polypeptides and antibodies of the present invention, including fragments thereof, may be used to detect Enterococcal species including E.faecalis using bio chip and biosensor technology. Bio chip and biosensors of the present invention may comprise the polypeptides of the present invention to detect antibodies, which specifically recognize Enterococcal species, including E.f tecalis. Bio chip and biosensors of the present invention may also comprise antibodies which specifically recognize the polypeptides of the present invention to detect Enterococcal species, including E. faecalis or specific polypeptides of the present invention. Bio chips or biosensors comprising polypeptides or antibodies of the present invention may be used to detect Enterococcal species, including E. faecalis, in biological and 15 environmental samples and to diagnose an animal, including humans, with an E.

faecalis or other Enterococcal infection. Thus, the present invention includes both bio chips and biosensors comprising polypeptides or antibodies of the present invention and methods of their use.

The bio chips of the present invention may further comprise polypeptide 20 sequences of other pathogens including bacteria, viral, parasitic, and fungal polypeptide sequences, in addition to the polypeptide sequences of the present invention, for use in rapid diffenertial pathogenic detection and diagnosis. The bio chips of the present invention may further comprise antibodies or fragements thereof specific for other pathogens including bacteria, viral, parasitic, and fungal polypeptide sequences, in addition to the antibodies or fragements thereof of the present invention, for use in rapid diffenertial pathogenic detection and diagnosis. The bio chips and biosensors of the present invention may also be used to monitor an E. faecalis or other Enterococcal infection and to monitor the genetic changes (amio acid deletions, insertions, substitutions, etc.) in response to drug therapy in the clinic and drug development in the laboratory. The bio chip and biosensors comprising polypeptides or antibodies of the present invention may also be used to simultaneously monitor the expression of a multiplicity of polypeptides, including those of the present invention.

The polypeptides used to comprise a bio chip or biosensor of the present invention may be specified in the same manner as for the fragements, i.e, by their N-tenninal and C-terminal positions or length in contigious amino acid residue. Methods and particular uses of the polypeptides and antibodies of the present invention to detect Enterococcal species, including E.faecalis, or specific polypeptides using bio chip and biosensor technology include those known in the art, those of the U.S. Patent Nos.

and World Patent Nos. listed above for bio chips and biosensors using polynucleotides of the present invention, and those of: U.S. Patent Nos. 5658732, 5135852, 5567301, 5677196, 5690894 and World Patent Nos. W09729366, W09612957, each incorporated herein in their entireties.

Treatment: Agonists and Antagonists Assays and Molecules.

•The invention also provides a method of screening compounds to identify those which enhance or block the biological activity of the E. faecalis polypeptides of the present invention. The present invention further provides where the compounds kill or slow the growth of E.faecalis. The ability of E.faecalis antagonists, including E. faecalis ligands, to prophylactically or therapeutically block antibiotic resistance may be easily tested by the skilled artisan. See, Straden et al. (1997) J Bacteriol.

179(1):9-16.

An agonist is a compound which increases the natural biological function or which functions in a manner similar to the polypeptides of the present invention, while antagonists decrease or eliminate such functions. Potential antagonists include small organic molecules, peptides, polypeptides, and antibodies that bind to a polypeptide of the invention and thereby inhibit or extinguish its activity.

The antagonists may be employed for instance to inhibit peptidoglycan cross bridge formation. Antibodies against E. faecalis may be employed to bind to and inhibit E. faecalis activity to treat antibiotic resistance. Any of the above antagonists may be employed in a composition with a pharmaceutically acceptable carrier.

Vaccines The present invention also provides vaccines comprising one or more polypeptides of the present invention. Heterogeneity in the composition of a vaccine may be provided by combining E. faecalis polypeptides of the present invention.

Multi-component vaccines of this type are desirable becau'se they are likely to be more effective in eliciting protective immune responses against multiple species and strains of the Enterococcus genus than single polypeptide vaccines.

Multi-component vaccines are known in the art to elicit antibody production to numerous immunogenic components. See, Decker et al. (1996) J. Infect. Dis.

174:S270-275. In addition, a hepatitis B, diphtheria, tetanus, pertussis tetravalent vaccine has recently been demonstrated to elicit protective levels of antibodies in human infants against all four pathogenic agents. See, Aristegui, J. et al. (1997) Vaccine 15:7-9.

The present invention in addition to single-component vaccines includes multi-component vaccines. These vaccines comprise more than one polypeptide, 20 immunogen or antigen. Thus, a multi-component vaccine would be a vaccine comprising more than one of the E. faecalis polypeptides of the present invention.

Further within the scope of the invention are whole cell and whole viral vaccines. Such vaccines may be produced recombinantly and involve the expression of one or more of the E. faecalis polypeptides described in Table 1. For example, the E. faecalis polypeptides of the present invention may be either secreted or localized intracellular, on ihe cell surface, or in the periplasmic space. Further, when a recombinant virus is used, the E. faecalis polypeptides of the present invention may, for example, be localized in the viral envelope, on the surface of the capsid, or internally within the capsid. Whole cells vaccines which employ cells expressing heterologous proteins are known in the art. See, Robinson, K. et al. (1997) Nature Biotech. 15:653-657; Sirard, J. et al. (1997) Infect. Immun. 65:2029-2033; Chabalgoity, J. et al. (1997) Infect. Immun. 65:2402-2412 These cells may be administered live or may be killed prior to administration. Chabalgoity, J. et al., supra, for example, report the successful use in mice of a live attenuated Salmonella vaccine strain which expresses a portion of a platyhelminth fatty acid-binding protein as a fusion protein on its cells surface.

A multi-component vaccine can also be prepared using techniques known in the art by combining one or more E. faecalis polypeptides of the present invention, or fragments thereof, with additional non-Enterococcal components diphtheria toxin or tetanus toxin, and/or other compounds known to elicit an immune response).

Such vaccines are useful for eliciting protective immune responses to both members of the Enterococcus genus and non-Enterococcal pathogenic agents.

The vaccines of the present invention also include DNA vaccines. DNA 15 vaccines are currently being developed for a number of infectious diseases. See, et al., Boyer, et al. (1997) Nat. Med. 3:526-532; reviewed in Spier, R. (1996) Vaccine 14:1285-1288. Such DNA vaccines contain a nucleotide sequence encoding one or more E. faecalis polypeptides of the present invention oriented in a manner that allows for expression of the subject polypeptide. For example, the direct administration of plasmid DNA encoding B. burgdorgeri OspA has been shown to elicit protective immunity in mice against borrelial challenge. See, Luke et al. (1997) J.

Infect. Dis. 175:91-97.

"The present invention also relates to the administration of a vaccine which is co-administered with a molecule capable of modulating immune responses. Kim et al.

(1997) Nature Biotech. 15:641-646, for example, report the enhancement of immune responses produced by DNA immunizations when DNA sequences encoding molecules which stimulate the immune response are co-administered. In a similar fashion, the vaccines of the present invention may be co-administered with either nucleic acids encoding immune modulators or the immune modulators themselves.

These immune modulators include granulocyte macrophage colony stimulating factor (GM-CSF) and CD86.

The vaccines of the present invention may be used to confer resistance to Enterococcal infection by either passive or active immunization. When the vaccines of the present invention are used to confer resistance to Enterococcal infection through active immunization, a vaccine of the present invention is administered to an animal to elicit a protective immune response which either prevents or attenuates a Enterococcal infection. When the vaccines of the present invention are used to confer resistance to Enterococcal infection through passive immunization, the vaccine is provided to a host animal human, dog, or mouse), and the antisera elicited by this antisera is recovered and directly provided to a recipient suspected of having an infection caused by a member of the Enterococcus genus.

The ability to label antibodies, or fragments of antibodies, with toxin molecules provides an additional method for treating Enterococcal infections when passive immunization is conducted. In this embodiment, antibodies, or fragments of antibodies, capable of recognizing the E.faecalis polypeptides disclosed herein, or fragments thereof, as well as other Enterococcus proteins, are labeled with toxin molecules prior to their administration to the patient. When such toxin derivatized antibodies bind to Enterococcus cells, toxin moieties will be localized to these cells and 20 will cause their death.

The present invention thus concerns and provides a means for preventing or attenuating a Enterococcal infection resulting from organisms which have antigens that are recognized and bound by antisera produced in response to the polypeptides of the present invention. As used herein, a vaccine is said to prevent or attenuate a disease if its administration to an animal results either in the total or partial attenuation suppression) of a symptom or condition of the disease, or in the total or partial immunity of the animal to the disease.

The administration of the vaccine (or the antisera which it elicits) may be for either a "prophylactic" or "therapeutic" purpose. When provided prophylactically, the compound(s) are provided in advance of any symptoms of Enterococcal infection.

The prophylactic administration of the compound(s) serves to prevent or attenuate any subsequent infection. When provided therapeutically, the compound(s) is provided upon or after the detection of symptoms which indicate that an animal may be infected with a member of the Enterococcus genus. The therapeutic administration of the compound(s) serves to attenuate any actual infection. Thus, the E. faecalis polypeptides, and fragments thereof, of the present invention may be provided either prior to the onset of infection (so as to prevent or attenuate an anticipated infection) or after the initiation of an actual infection.

The polypeptides of the invention, whether encoding a portion of a native protein or a functional derivative thereof, may be administered in pure form or may be coupled to a macromolecular carrier. Example of such carriers are proteins and carbohydrates. Suitable proteins which may act as macromolecular carrier for "enhancing the immunogenicity of the polypeptides of the present invention include 15 keyhole limpet hemacyanin (KLH) tetanus toxoid, pertussis toxin, bovine serum albumin, and ovalbumin. Methods for coupling the polypeptides of the present invention to such macromolecular carriers are disclosed in Harlow et al., ANTIBODIES: A LABORATORY MANUAL, (Cold Spring Harbor Laboratory Press, 2nd ed. 1988).

A composition is said to be "pharmacologically or physiologically acceptable" if its administration can be tolerated by a recipient animal and is otherwise suitable for administration to that animal. Such an agent is said to be administered in a "therapeutically effective amount" if the amount administered is physiologically significant. An agent is physiologically significant if its presence results in a detectable change in the physiology of a recipient patient.

While in all instances the vaccine of the present invention is administered as a pharmacologically acceptable compound, one skilled in the art would recognize that the composition of a pharmacologically acceptable compound varies with the animal to which it is administered. For example, a vaccine intended for human use will generally not be co-administered with Freund's adjuvant. Further, the level of purity of the E. faecalis polypeptides of the present invention will normally be higher when administered to a human than when administered to a non-human animal.

As would be understood by one of ordinary skill in the art, when the vaccine of the present invention is provided to an animal, it may be in a composition which may contain salts, buffers, adjuvants, or other substances which are desirable for improving the efficacy of the composition. Adjuvants are substances that can be used to specifically augment a specific immune response. These substances generally perform two functions: they protect the antigen(s) from being rapidly catabolized after administration and they nonspecifically stimulate immune responses.

Normally, the adjuvant and the composition are mixed prior to presentation to the immune system, or presented separately, but into the same site of the animal being immunized. Adjuvants can be loosely divided into several groups based upon their composition. These groups include oil adjuvants (for example, Freund's complete and 15 incomplete), mineral salts (for example, AIK(S0 4 2 AINa(S0 4 2

AINH

4 (SO4), silica, kaolin, and carbon), polynucleotides (for example, poly IC and poly AU acids), and certain natural substances (for example, wax D from Mycobacterium tuberculosis, as well as substances found in Corynebacterium parvum, or Bordetella pertussis, and members of the genus Brucella. Other substances useful as adjuvants are the saponins such as, for example, Quil A. (Superfos A/S, Denmark). Preferred adjuvants for use in the present invention include aluminum salts, such as AlK(SO 4 2 AINa(SO4)2, and S: AINH 4 (SO4). Examples of materials suitable for use in vaccine compositions are provided in REMINGTON'S PHARMACEUTICAL SCIENCES 1324-1341 (A.

Osol, ed, Mack Publishing Co, Easton, PA, (1980) (incorporated herein by reference).

The therapeutic compositions of the present invention can be administered parenterally by injection, rapid infusion, nasopharyngeal absorption (intranasopharangeally), dermoabsorption, or orally. The compositions may alternatively be administered intramuscularly, or intravenously. Compositions for parenteral administration include sterile aqueous or non-aqueous solutions, suspensions, and emulsions. Examples of non-aqueous solvents are propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable organic esters such as ethyl oleate. Carriers or occlusive dressings can be used to increase skin permeability and enhance antigen absorption. Liquid dosage forms for oral administration may generally comprise a liposome solution containing the liquid dosage form. Suitable forms for suspending liposomes include emulsions, suspensions, solutions, syrups, and elixirs containing inert diluents commonly used in the art, such as purified water. Besides the inert diluents, such compositions can also include adjuvants, wetting agents, emulsifying and suspending agents, or sweetening, I flavoring, or perfuming agents.

Therapeutic compositions of the present invention can also be administered in S* encapsulated form. For example, intranasal immunization using vaccines encapsulated in biodegradable microsphere composed of poly(DL-lactide-co-glycolide). See, Shahin, R. et al. (1995) Infect. Immun. 63:1195-1200. Similarly, orally administered encapsulated Salmonella typhimurium antigens can also be used. Allaoui-Attarki, K.

et al. (1997) Infect. Immun. 65:853-857. Encapsulated vaccines of the present ~invention can be administered by a variety of routes including those involving contacting the vaccine with mucous membranes intranasally, intracolonicly, intraduodenally).

20 Many different techniques exist for the timing of the immunizations when a multiple administration regimen is utilized. It is possible to use the compositions of the invention more than once to increase the levels and diversities of expression of the immunoglobulin repertoire expressed by the immunized animal. Typically, if multiple immunizations are given, they will be given one to two months apart.

According to the present invention, an "effective amount" of a therapeutic composition is one which is sufficient to achieve a desired biological effect Generally, the dosage needed to provide an effective amount of the composition will vary depending upon such factors as the animal's or human's age, condition, sex, and extent of disease, if any, and other variables which can be adjusted by one of ordinary skill in the art.

The antigenic preparations of the invention can be administered by either single or multiple dosages of an effective amount. Effective amounts of the compositions of the invention can vary from 0.01-1,000 tg/ml per dose, more preferably 0.1-500 pg/ml per dose, and most preferably 10-300 .g/ml per dose.

Examples Example 1: Isolation of a Selected DNA Clone From the Deposited Sample ofE.

faecalis I Three approaches can be used to isolate a E. faecalis clone comprising a polynucleotide of the present invention from any E.faecalis genomic DNA library.

The E. faecalis strain V586 has been deposited as a convienent source for obtaining a E. faecalis strain although a wide varity of strains E. faecalis strains can be used which are known in the art.

E. faecalis genomic DNA is prepared using the following method. A overnight bacterial culture grown in a rich medium Trypticase Soy Broth, Brain Heart Infusion broth or Super broth), pelleted, washed two times with TES Tris-pH 8.0, 25mM EDTA, 50mM NaCI), and resuspended in 5ml high salt TES NaCI). Lysostaphin is added to final concentration of approx 50ug/ml and the 20 mixture is rotated slowly 1 hour at 37C to make protoplast cells. The solution is then placed in incubator (or place in a shaking water bath) and warmed to 55C. Five hundred micro liter of 20% sarcosyl in TES (final concentration is then added to lyse the cells. Next, guanidine HCI is added to a final concentration of7M (3.69g in ml). The mixture is swirled slowly at 55C for 60-90 min (solution should clear).

A CsCI gradient is then set up in SW41 ultra clear tubes using 2.0ml 5.7M CsCI and overlaying with 2.85M CsCI. The gradient is carefully overlayed with the DNAcontaining GuHCI solution. The gradient is spun at 30,000 rpm, 20C for 24 hr and the lower DNA band is collected. The volume is increased to 5 ml with TE buffer.

The DNA is then treated with protease K (10 ug/ml) overnight at 37 C, and precipitated with ethanol. The precipitated DNA is resuspended in a desired buffer.

In the first method, a plasmid is directly isolated by screening a plasmid E.

faecalis genomic DNA library using a polynucleotide probe corresponding to a polynucleotide of the present invention. Particularly, a specific polynucleotide with 30-40 nucleotides is synthesized using an Applied Biosystems DNA synthesizer according to the sequence reported. The oligonucleotide is labeled, for instance, with 3 2 P-y-ATP using T4 polynucleotide kinase and purified according to routine methods.

(See, Maniatis et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Press, Cold Spring, NY (1982).) The library is transformed into a suitable host, as indicated above (such as XL-I Blue (Stratagene)) using techniques known to those of skill in the art. See, Sambrook et al. MOLECULAR CLONING: A LABORATORY MANUAL (Cold Spring Harbor, N.Y. 2nd ed. 1989); Ausubel et al., CURRENT PROTOCALS IN MOLECULAR BIOLOGY (John Wiley and Sons, N.Y. 1989). The transformants are plated on 1.5% agar plates (containing the 15 appropriate selection agent, ampicillin) to a density of about 150 transformants (colonies) per plate. These plates are screened using Nylon membranes according to routine methods for bacterial colony screening. See, Sambrook et al.

MOLECULAR CLONING: A LABORATORY MANUAL (Cold Spring Harbor, N.Y. 2nd ed. 1989); Ausubel et al., CURRENT PROTOCALS IN MOLECULAR BIOLOGY (John Wiley and Sons, N.Y. 1989) or other techniques known to those of skill in the art.

Alternatively, two primers of 15-25 nucleotides derived from the 5' and 3' ends of a polynucleotide of Table 1 are synthesized and used to amplify the desired DNA by PCR using a E. faecalis genomic DNA prep as a template. PCR is carried out under routine conditions, for instance, in 25 pl of reaction mixture with 0.5 ug of the above DNA template. A convenient reaction mixture is 1.5-5 mM MgCI 2 0.01% gelatin, 20 tM each of dATP, dCTP, dGTP, dTTP, 25 pmol of each primer and 0.25 Unit of Taq polymerase. Thirty five cycles of PCR (denaturation at 94C for 1 min; annealing at 55 0 C for 1 min; elongation at 72 0 C for 1 min) are performed with a Perkin-Elmer Cetus automated thermal cycler. The amplified product is analyzed by agarose gel electrophoresis and the DNA. band with expected molecular weight is excised and purified. The PCR product is verified to be the selected sequence by subcloning and sequencing the DNA product.

Finally, overlapping oligos of the DNA sequences of Table 1 can be chemically synthesized and used to generate a nucleotide sequence of desired length using PCR methods known in the art.

Example Expression and Purification Enlerococcal polypeptides in E. coli The bacterial expression vector pQE60 was used for bacterial expression of some of the polypeptide fragements used in the soft tissue and systemic infection models discussed below. (QIAGEN, Inc., 9259 Eton Avenue, Chatsworth, CA, 91311). pQE60 encodes ampicillin antibiotic resistance ("Ampr") and contains a S" bacterial origin of replication an IPTG inducible promoter, a ribosome binding site six codons encoding histidine residues that allow affinity purification using nickel-nitrilo-tri-acetic acid ("Ni-NTA") affinity resin (QIAGEN, Inc., supra) ~and suitable single restriction enzyme cleavage sites. These elements are arranged such that an inserted DNA fragment encoding a polypeptide expresses that polypeptide with the six His residues a "6 X His tag") covalently linked to the carboxyl terminus of that polypeptide.

The DNA sequence encoding the desired portion of a E. faecalis protein of the present invention was amplified from E. faecalis genomic DNA using PCR oligonucleotide primers which anneal to the 5' and 3' sequences coding for the portions of the E. faecalis polynucleotide shown in Table 1. Additional nucleotides containing restriction sites to facilitate cloning in the pQE60 vector are added to the and 3' sequences, respectively.

For cloning the mature protein, the 5' primer has a sequence containing an appropriate restriction site followed by nucleotides of the amino terminal coding sequence of the desired E. faecalis polynucleotide sequence in Table 1. One of ordinary skill in the art would appreciate that the point in the protein coding sequence where the 5' and 3' primers begin may be varied to amplify a DNA segment encoding any desired portion of the complete protein shorter or longer than the mature form.

The 3' primer has a sequence containing an appropriate restriction site followed by nucleotides complementary to the 3' end of the polypeptide coding sequence of Table 1, excluding a stop codon, with the coding sequence aligned with the restriction site so as to maintain its reading frame with that of the six His codons in the pQE60 vector.

The amplified E. faecalis DNA fragment and the vector pQE60 were digested with restriction enzymes which recognize the sites in the primers and the digested 0o DNAs were then ligated together. The E. faecalis DNA was inserted into the restricted pQE60 vector in a manner which places the E. faecalis protein coding region downstream from the IPTG-inducible promoter and in-frame with an initiating AUG and the six histidine codons.

The ligation mixture was transformed into competent E. coli cells using 15 standard procedures such as those described by Sambrook et al., supra.. E. coli strain M15/rep4, containing multiple copies of the plasmid pREP4, which expresses the lac repressor and confers kanamycin resistance was used in carrying out the illustrative example described herein. This strain, which was only one of many that are suitable for expressing a E. faecalis polypeptide, is available commercially 20 (QIAGEN, Inc., supra). Transformants were identified by their ability to grow on LB agar plates in the presence of ampicillin and kanamycin. Plasmid DNA was isolated from resistant colonies and the identity of the cloned DNA confirmed by restriction analysis, PCR and DNA sequencing.

Clones containing the desired constructs were grown overnight in liquid culture in LB media supplemented with both ampicillin (100 .tg/ml) and kanamycin (25 tg/ml). The O/N'culture was used to inoculate a large culture, at a dilution of approximately 1:25 to 1:250. The cells were grown to an optical density at 600 nm ("OD600") of between 0.4 and 0.6. Isopropyl-p-D-thiogalactopyranoside ("IPTG") was then added to a final concentration of 1 mM to induce transcription from the lac repressor sensitive promoter, by inactivating the lad repressor. Cells subsequently were incubated further for 3 to 4 hours. Cells then were harvested by centrifugation.

The cells were then stirred for 3-4 hours at 4 0 C in 6M guanidine-HC1, pH 8.

The cell debris was removed by centrifugation, and the supernatant containing the E.

faecalis polypeptide was loaded onto a nickel-nitrilo-tri-acetic acid ("Ni-NTA") affinity resin column (Q1AGEN, Inc., supra). Proteins with a 6 x His tag bind to the Ni-NTA resin with high affinity were purified in a simple one-step procedure (for details see: The QlAexpressionist, 1995, QIAGEN, Inc., supra). Briefly the supernatant was loaded onto the column in 6 M guanidine-HC1, pH 8, the column was first washed with 10 volumes of 6 M guanidine-HCl, pH 8, then washed with volumes of 6 M guanidine-HCI pH 6, and finally the E.faecalis polypeptide was eluted with 6 M guanidine-HCI, pH The purified protein was then renatured by dialyzing it against 15 phosphate-buffered saline (PBS) or 50 mM Na-acetate, pH 6 buffer plus 200 mM NaCI. Alternatively, the protein could be successfully refolded while immobilized on the Ni-NTA column. The recommended conditions are as follows: renature using a linear 6M-1M urea gradient in 500 mM NaCI, 20% glycerol, 20 mM Tris/HC1 pH 7.4, containing protease inhibitors. The renaturation should be performed over a period of 1.5 hours or more. After renaturation the proteins can be eluted by the addition of 250 mM immidazole. Immidazole was removed by a final dialyzing step against PBS or 50 mM sodium acetate pH 6 buffer plus 200 mM NaCI. The purified protein was stored at 40 C or frozen at -800 C.

Some of the polypeptide of the present invention were prepared using a nondenaturing protein purification method. For these polypeptides, the cell pellet from each liter of culture was resuspended in 25 mis of Lysis Buffer A at 4 0 C (Lysis Buffer A 50 mM Na-phosphate, 300 mM NaCI, 10 mM 2-mercaptoethanol, Glycerol, pH 7.5 with 1 tablet of Complete EDTA-free protease inhibitor cocktail (Boehringer Mannheim #1873580) per 50 ml of buffer). Absorbance at 550 nm was approximately 10-20 O.D./ml. The suspension was then put through three freeze/thaw cycles from -70 0 C (using a ethanol-dry ice bath) up to room temperature.

The cells were lysed via sonication in short 10 sec bursts over 3 minutes at approximately 80W while kept on ice. The sonicated sample was then centrifuged at 15,000 RPM for 30 minutes at 4°C. The supernatant was passed through a column containing 1.0 ml of CL-4B resin to pre-clear the sample of any proteins that may bind to agarose non-specifically, and the flow-through fraction was collected.

The pre-cleared flow-through was applied to a nickel-nitrilo-tri-acetic acid ("Ni-NTA") affinity resin column (Quiagen, Inc., supra). Proteins with a 6 X His tag to bind to the Ni-NTA resin with high affinity and can be purified in a simple one-step procedure. Briefly, the supernatant was loaded onto the column in Lysis Buffer A at 4 0 C, the column was first washed with 10 volumes of Lysis Buffer A until the A280 of the eluate returns to the baseline. Then, the column was washed with 5 volumes of S 40 mM Imidazole (92% Lysis Buffer A 8% Buffer B) (Buffer B 50 mM Na- 15 Phosphate, 300 mM NaCI, 10% Glycerol, 10 mM 2-mercaptoethanol, 500 mM Imidazole, pH of the final buffer should be The protein was eluted off of the column with a series of increasing Imidazole solutions made by adjusting the ratios of Lysis Buffer A to Buffer B. Three different concentrations were used: 3 volumes of 75 mM Imidazole, 3 volumes of 150 mM Imidazole, 5 volumes of 500 mM 20 Imidazole. The fractions containing the purified protein were analyzed using 8 or 14% SDS-PAGE depending on the protein size. The purified protein was then dialyzed 2X against phosphate-buffered saline (PBS) in order to place it into an easily o workable buffer. The purified protein was stored at 40 C or frozen at The following alternative method may be used to purify E.faecalis expressed in E coli when it is present in the form of inclusion bodies. Unless otherwise specified, all of the following steps are conducted at 4-10 0

C.

Upon completion of the production phase of the E. coli fermentation, the cell culture is cooled to 4-10 0 C and the cells are harvested by continuous centrifugation at 15,000 rpm (Heraeus Sepatech). On the basis of the expected yield of protein per unit weight of cell paste and the amount of purified protein required, an appropriate amount of cell paste, by weight, is suspended in a buffer solution containing 100 mM Tris, 50 mM EDTA, pH 7.4. The cells are dispersed to a homogeneous suspension using a high shear mixer.

The cells are then lysed by passing the solution through a microfluidizer (Microfuidics, Corp. or APV Gaulin, Inc.) twice at 4000-6000 psi. The homogenate is then mixed with NaCI solution to a final concentration of 0.5 M NaCI, followed by centrifugation at 7000 x g for 15 min. The resultant pellet is washed again using NaCI, 100 mM Tris, 50 mM EDTA, pH 7.4.

o1 The resulting washed inclusion bodies are solubilized with 1.5 M guanidine hydrochloride (GuHCI) for 2-4 hours. After 7000 x g centrifugation for 15 min., the *e pellet is discarded and the E.faecalis polypeptide-containing supernatant is incubated

S.

at 4 0 C overnight to allow further GuHCl extraction.

Following high speed centrifugation (30,000 x g) to remove insoluble particles, 15 the GuHCI solubilized protein is refolded by quickly mixing the GuHCl extract with volumes of buffer containing 50 mM sodium, pH 4.5, 150 mM NaCI, 2 mM se..: EDTA by vigorous stirring. The refolded diluted protein solution is kept at 4°C without mixing for 12 hours prior to further purification steps.

To clarify the refolded E. faecalis polypeptide solution, a previously prepared tangential filtration unit equipped with 0.16 ptm membrane filter with appropriate surface area Filtron), equilibrated with 40 mM sodium acetate, pH 6.0 is employed. The filtered sample is loaded onto a cation exchange resin Poros HS- Perseptive Biosystems). The column is washed with 40 mM sodium acetate, pH and eluted with 250 mM, 500 mM, 1000 mM, and 1500 mM NaCI in the same buffer, in a stepwise manner. The absorbance at 280 mm of the effluent is continuously monitored. Fractions are collected and further analyzed by SDS-PAGE.

Fractions containing the E.faecalis polypeptide are then pooled and mixed with 4 volumes of water. The diluted sample is then loaded onto a previously prepared set of tandem columns of strong anion (Poros HQ-50, Perseptive Biosystems) and weak anion (Poros CM-20, Perseptive Biosystems) exchange resins.

The columns are equilibrated with 40 mM sodium acetate, pH 6.0. Both columns are washed with 40 mM sodium acetate, pH 6.0, 200 mM NaCI. The CM-20 column is then eluted using a 10 column volume linear gradient ranging from 0.2 M NaC1, mM sodium acetate, pH 6.0 to 1.0 M NaC1, 50 mM sodium acetate, pH Fractions are collected under constant A 28 o monitoring of the effluent. Fractions containing the E. faecalis polypeptide (determined, for instance, by 16% SDS-PAGE) are then pooled.

The resultant E. faecalis polypeptide exhibits greater than 95% purity after the above refolding and purification steps. No major contaminant bands are observed from Commassie blue stained 16% SDS-PAGE gel when 5 p-g of purified protein is loaded. The purified protein is also tested for endotoxin/LPS contamination, and typically the LPS content is less than 0.1 ng/ml according to LAL assays.

i 15 Example Alternative Expression and Purification Enterococcalpolypeptides in E.

coli Tthe vector pQE 10 was alternatively used to clone and express some of the polypeptides of the present invention for use in the soft tissue and systemic infection models discussed below. The difference being such that an inserted DNA fragment 20 encoding a polypeptide expresses that polypeptide with the six His residues a "6 X His tag") covalently linked to the amino terminus of that polypeptide. The bacterial expression vector pQE10 (QIAGEN, Inc., 9259 Eton Avenue, Chatsworth, CA, 91311) was used in this example. The components of the pQE 10 plasmid are arranged such that the inserted DNA sequence encoding a polypeptide of the present invention expresses the polypeptide with the six His residues a "6 X His tag")) covalently linked to the amino terminus.

The DNA sequences encoding the desired portions of a polypeptide of Table 1 were amplified using PCR oligonucleotide primers from genomic E. faecalis DNA.

The PCR primers anneal to the nucleotide sequences encoding the desired amino acid sequence of a polypeptide of the present invention. Additional nucleotides containing restriction sites to facilitate cloning in the pQE 10 vector were added to the 5' and 3' primer sequences, respectively.

For cloning a polypeptide of the present invention, the 5' and 3' primers were selected to amplify their respective nucleotide coding sequences. One of ordinary skill in the art would appreciate that the point in the protein coding sequence where the and 3' primers begins may be varied to amplify a DNA segment encoding any desired portion of a polypeptide of the present invention. The 5' primer was designed so the coding sequence of the 6 X His tag is aligned with the resiriction site so as to maintain its reading frame with that of E. faecalis polypeptide. The 3' was designed to include an stop codon. The amplified DNA fragment was then cloned, and the protein expressed, as described above for the pQE60 plasmid.

The DNA sequences encoding the amino acid sequences of Table 1 may also be cloned and expressed as fusion proteins by a protocol similar to that described 15 directly above, wherein the pET-32b(+) vector (Novagen, 601 Science Drive, Madison, WI 53711) is preferentially used in place of pQElO.

The above methods are not limited to the polypeptide fragements actually produced. The above method, like the methods below, can be used to produce either full length polypeptides or desired fragements therof.

Example Alternative Expression and Purification of Enterococcal polypeptides in E. coli The bacterial expression vector pQE60 is used for bacterial expression in this example (QIAGEN, Inc., 9259 Eton Avenue, Chatsworth, CA, 91311). However, in this example, the polypeptide coding sequence is inserted such that translation of the six His codons is prevented and, therefore, the polypeptide is produced with no 6 X His tag.

The DNA sequence encoding the desired portion of the E. faecalis amino acid sequence is amplified from an E. faecalis genomic DNA prep the deposited DNA clones using PCR oligonucleotide primers which anneal to the 5' and 3' nucleotide sequences corresponding to the desired portion of the E. faecalis polypeptides.

Additional nucleotides containing restriction sites to facilitate cloning in the vector are added to the 5' and 3' primer sequences.

For cloning a E. faecalis polypeptides of the present invention, 5' and 3' primers are selected to amplify their respective nucleotide coding sequences. One of ordinary skill in the art would appreciate that the point in the protein coding sequence where the 5' and 3' primers begin may be varied to amplify a DNA segment encoding any desired portion of a polypeptide of the present invention. The 3' and 5' primers contain appropriate restriction sites followed by nucleotides complementary to the and 3' ends of the coding sequence respectively. The 3' primer is additionally designed to include an in-frame stop codon.

The amplified E. faecalis DNA fragments and the vector pQE60 are digested with restriction enzymes recognizing the sites in the primers and the digested DNAs are then ligated together. Insertion of the E.faecalis DNA into the restricted vector places the E. faecalis protein coding region including its associated stop codon downstream from the IPTG-inducible promoter and in-frame with an initiating AUG.

The associated stop codon prevents translation of the six histidine codons downstream of the insertion point.

The ligation mixture is transformed into competent E. coli cells using standard procedures such as those described by Sambrook et al. E. coli strain M 15/rep4, containing multiple copies of the plasmid pREP4, which expresses the lac repressor S* and confers kanamycin resistance is used in carrying out the illustrative example described herein. This strain, which is only one of many that are suitable for expressing E. faecalis polypeptide, is available commercially (QIAGEN, Inc., supra).

Transformants are identified by their ability to grow on LB plates in the presence of ampicillin and kanamycin. Plasmid DNA is isolated from resistant colonies and the identity of the cloned DNA confirmed by restriction analysis, PCR and DNA sequencing.

Clones containing the desired constructs are grown overnight in liquid culture in LB media supplemented with both ampicillin (100 .g/ml) and kanamycin g/ml). The O/N culture is used to inoculate a large culture, at a dilution of approximately 1:25 to 1:250. The cells are grown to an optical density at 600 nm ("OD600") of between 0.4 and 0.6. isopropyl-b-D-thiogalactopyranoside ("IPTG") is then added to a final concentration of 1 mM to induce transcription from the lac repressor sensitive promoter, by inactivating the lad repressor. Cells subsequently are incubated further for 3 to 4 hours. Cells then are harvested by centrifugation.

To purify the E faecalis polypeptide, the cells are then stirred for 3-4 hours at 4 0 C in 6M guanidine-HC1, pH 8. The cell debris is removed by centrifugation, and the supernatant containing the E. faecalis polypeptide is dialyzed against 50 mM Naacetate buffer pH 6, supplemented with 200 mM NaCI. Alternatively, the protein can be successfully refolded by dialyzing it against 500 mM NaCI, 20% glycerol, mM Tris/HC1 pH 7.4, containing protease inhibitors. After renaturation the protein 15 can be purified by ion exchange, hydrophobic interaction and size exclusion chromatography. Alternatively, an affinity chromatography step such as an antibody column can be used to obtain pure E. faecalis polypeptide. The purified protein is stored at 40 C or frozen at -800 C.

The following alternative method may be used to purify E. faecalis 20 polypeptides expressed in E coli when it is present in the form of inclusion bodies.

Unless otherwise specified, all of the following steps are conducted at 4-10 0

C.

Upon completion of the production phase of the E. coli fermentation, the cell culture is cooled to 4-10°C and the cells are harvested by continuous centrifugation at 15,000 rpm (Heraeus Sepatech). On the basis of the expected yield of protein per unit weight of cell paste and the amount of purified protein required, an appropriate amount of cell paste, by weight, is suspended in a buffer solution containing 100 mM Tris, 50 mM EDTA, pH 7.4. The cells are dispersed to a homogeneous suspension using a high shear mixer.

The cells ware then lysed by passing the solution through a microfluidizer (Microfuidics, Corp. or APV Gaulin, Inc.) twice at 4000-6000 psi. The homogenate is then mixed with NaCI solution to a final concentration of 0.5 M NaCI, followed by centrifugation at 7000 x g for 15 min. The resultant pellet is washed again using NaCI, 100 mM Tris, 50 mM EDTA, pH 7.4.

The resulting washed inclusion bodies are solubilized with 1.5 M guanidine hydrochloride (GuHCI) for 2-4 hours. After 7000 x g centrifugation for 15 min., the pellet is discarded and the E. faecalis polypeptide-containing supematant is incubated at 4 0 C overnight to allow further GuHCI extraction.

Following high speed centrifugation (30,000 x g) to remove insoluble particles, in the GuHCI solubilized protein is refolded by quickly mixing the GuHCI extract with volumes of buffer containing 50 mM sodium, pH 4.5, 150 mM NaCI, 2 mM EDTA by vigorous stirring. The refolded diluted protein solution is kept at 4 C without mixing for 12 hours prior to further purification steps.

To clarify the refolded E. faecalis polypeptide solution, a previously prepared tangential filtration unit equipped with 0.16 p.m membrane filter with appropriate surface area Filtron), equilibrated with 40 mM sodium acetate, pH 6.0 is employed. The filtered sample is loaded onto a cation exchange resin Poros HS- Perseptive Biosystems). The column is washed with 40 mM sodium acetate, pH 6.0 and eluted with 250 mM, 500 mM, 1000 mM, and 1500 mM NaCI in the same 20 buffer, in a stepwise manner. The absorbance at 280 mm of the effluent is continuously monitored. Fractions are collected and further analyzed by SDS-PAGE.

Fractions containing the E.faecalis polypeptide are then pooled and mixed with 4 volumes of water. The diluted sample is then loaded onto a previously prepared set of tandem columns of strong anion (Poros HQ-50, Perseptive Biosystems) and weak anion (Poros CM-20, Perseptive Biosystems) exchange resins.

The columns are equilibrated with 40 mM sodium acetate, pH 6.0. Both columns are washed with 40 mM sodium acetate, pH 6.0, 200 mM NaCI. The CM-20 column is then eluted using a 10 column volume linear gradient ranging from 0.2 M NaCI, mM sodium acetate, pH 6.0 to 1.0 M NaCI, 50 mM sodium acetate, pH Fractions are collected under constant Azso monitoring of the effluent. Fractions containing the E. faecalis polypeptide (determined, for instance, by 16% SDS-PAGE) are then pooled.

The resultant E. aecalis polypeptide exhibits greater than 95% purity after the above refolding and purification steps. No major contaminant bands are observed from Commassie blue stained 16% SDS-PAGE gel when 5 p g of purified protein is loaded. The purified protein is also tested for endotoxin/LPS contamination, and typically the LPS content is less than 0.1 ng/ml according to LAL assays.

Example Cloning and Expression of E. faecalis in Other Bacteria E.faecalis polypeptides can also be produced in: E. faecalis using the methods of S. Skinner et al., (1988) Mol. Microbiol. 2:289-297 or J. 1. Moreno (1996) Protein Expr. Purif. 8(3):332-340; Lactobacillus using the methods of C. Rush et al., 1997 Appl. Microbiol. Biotechnol. 47(5):537-542; or in Bacillus subtilis using the methods Chang et al., U.S. Patent No. 4,952,508.

Example 3: Cloning and Expression in COS Cells A E. faecalis expression plasmid is made by cloning a portion of the DNA encoding a E. faecalis polypeptide into the expression vector pDNAI/Amp or pDNAIII (which can be obtained from Invitrogen, Inc.). The expression vector pDNAI/amp contains: an E. coli origin of replication effective for propagation in E. coli and other prokaryotic cells; an ampicillin resistance gene for selection of S: plasmid-containing prokaryotic cells; an SV40 origin of replication for propagation in eukaryotic cells; a CMV promoter, a polylinker, an SV40 intron; several codons encoding a hemagglutinin fragment an "HA" tag to facilitate purification) followed by a termination codon and polyadenylation signal arranged so that a DNA can be conveniently placed under expression control of the CMV promoter and operably linked to the SV40 intron and the polyadenylation signal by means of restriction sites in the polylinker. The HA tag corresponds to an epitope derived from the influenza hemagglutinin protein described by Wilson et al. 1984 Cell 37:767.

The fusion of the HA tag to the target protein allows easy detection and recovery of the rccombinant protein with an antibody that recognizes the HA epitope. pDNAIII contains, in addition, the selectable neomycin marker.

A DNA fragment encoding a E. faecalis polypeptide is cloned into the polylinker region of the vector so that recombinant protein expression is directed by the CMV promoter. The plasmid construction strategy is as follows. The DNA from a E. faecalis genomic DNA prep is amplified using primers that contain convenient restriction sites, much as described above for construction of vectors for expression of E. faecalis in E. coli. The 5' primer contains a Kozak sequence, an AUG start codon, and nucleotides of the 5' coding region of the E. faecalis polypeptide. The 3' primer, contains nucleotides complementary to the 3' coding sequence of the E. faecalis DNA, a stop codon, and a convenient restriction site.

**The PCR amplified DNA fragment and the vector, pDNAI/Amp, are digested 15 with appropriate restriction enzymes and then ligated. The ligation mixture is transformed into an appropriate E. coli strain such as SURE T (Stratagene Cloning Systems, La Jolla, CA 92037), and the transformed culture is plated on ampicillin a media plates which then are incubated to allow growth of ampicillin resistant colonies.

Plasmid DNA is isolated from resistant colonies and examined by restriction analysis 20 or other means for the presence of the fragment encoding the E. faecalis polypeptide For expression of a recombinant E. faecalis polypeptide, COS cells are transfected with an expression vector, as described above, using DEAE-dextran, as described, for instance, by Sambrook et al. (supra). Cells are incubated under conditions for expression of E. faecalis by the vector.

Expression of the E. faecalis-HA fusion protein is detected by radiolabeling and immunoprecipitation, using methods described in, for example Harlow et al., supra.. To this end, two days after transfection, the cells are labeled by incubation in media containing "S-cysteine for 8 hours. The cells and the media are collected, and the cells are washed and the lysed with detergent-containing RIPA buffer: 150 mM NaCI, 1% NP-40, 0.1% SDS, 1% NP-40, 0.5% DOC, 50 mM TRIS, pH 7.5, as described by Wilson et al. (supra Proteins are precipitated from the cell lysate and from the culture media using an HA-specific monoclonal antibody. The precipitated proteins then are analyzed by SDS-PAGE and autoradiography. An expression product of the expected size is seen in the cell lysate, which is not seen in negative controls.

Example 4: Cloning and Expression in CHO Cells The vector pC4 is used for the expression of E. fdecalis polypeptide in this example. Plasmid pC4 is a derivative of the plasmid pSV2-dhfr (ATCC Accession No. 37146). The plasmid contains the mouse DHFR gene under control of the early promoter. Chinese hamster ovary cells or other cells lacking dihydrofolate activity that are transfected with these plasmids can be selected by growing the cells in a selective medium (alpha minus MEM, Life Technologies) supplemented with the 15 chemotherapeutic agent methotrexate. The amplification of the DHFR genes in cells resistant to methotrexate (MTX) has been well documented. See, Alt et al., 1978, J. Biol. Chem. 253:1357-1370; Hamlin et al., 1990, Biochem. et Biophys. Acta, 1097:107-143; Page et al., 1991, Biotechnology 9:64-68. Cells grown in increasing concentrations of MTX develop resistance to the drug by overproducing the target 20 enzyme, DHFR, as a result of amplification of the DHFR gene. If a second gene is linked to the DHFR gene, it is usually co-amplified and over-expressed. It is known in the art that this approach may be used to develop cell lines carrying more than S1,000 copies of the amplified gene(s). Subsequently, when the methotrexate is withdrawn, cell lines are obtained which contain the amplified gene integrated into one or more chromosome(s) of the host cell.

Plasmid pC4 contains the strong promoter of the long terminal repeat (LTR) of the Rouse Sarcoma Virus, for expressing a polypeptide of interest, Cullen, et al.

(1985) Mol. Cell. Biol. 5:438-447; plus a fragment isolated from the enhancer of the immediate early gene of human cytomegalovirus (CMV), Boshart, et al., 1985, Cell 41:521-530. Downstream of the promoter are the following single restriction enzyme cleavage sites that allow the integration of the genes: Barn HI, Xba 1, and Asp 718.

Behind these cloning sites the plasmid contains the 3' intron and polyadenylation site of the rat preproinsulin gene. Other high efficiency promoters can also be used for the expression, the human 8-actin promoter, the SV40 early or late promoters or the long terminal repeats from other retroviruses, HIV and HTLVI. Clontech's Tet- Off and Tet-On gene expression systems and similar systems can be used to express the E. faecalis polypeptide in a regulated way in mammalian cells (Gossen et al., 1992, Proc. Natl. Acad. Sci. USA 89:5547-5551. For the polyadenylation of the mRNA other signals, from the human growth hormone or globin genes can be used as well. Stable cell lines carrying a gene of interest integrated into the chromosomes can also be selected upon co-transfection with a selectable marker such as gpt, G418 or hygromycin. It is advantageous to use more than one selectable marker in the beginning, G418 plus methotrexate.

15 The plasmid pC4 is digested with the restriction enzymes and then dephosphorylated using calf intestinal phosphates by procedures known in the art.

The vector is then isolated from a 1% agarose gel. The DNA sequence encoding the E.

faecalis polypeptide is amplified using PCR oligonucleotide primers corresponding to the 5' and 3' sequences of the desired portion of the gene. A 5' primer containing a restriction site, a Kozak sequence, an AUG start codon, and nucleotides of the coding region of the E. faecalis polypeptide is synthesized and used. A 3' primer, containing a restriction site, stop codon, and nucleotides complementary to the 3' coding sequence of the E.faecalis polypeptides is synthesized and used. The amplified fragment is digested with the restriction endonucleases and then purified again on a 1% agarose gel. The isolated fragment and the dephosphorylated vector are then ligated with T4 DNA ligase. E. coli HB 101 or XL-I Blue cells are then transformed and bacteria are identified that contain the fragment inserted into plasmid pC4 using, for instance, restriction enzyme analysis.

Chinese hamster ovary cells lacking an active DHFR gene are used for transfection. Five .g of the expression plasmid pC4 is cotransfected with 0.5 p.g of the plasmid pSVneo using a lipid-mediated transfection agent such as Lipofectin or LipofectAMINE.

T (LifeTechnologies Gaithersburg, MD). The plasmid pSV2-neo contains a dominant selectable marker, the neo gene from Tn5 encoding an enzyme that confers resistance to a group of antibiotics including G418. The cells are seeded in alpha minus MEM supplemented with 1 mg/ml G418. After 2 days, the cells are trypsinized and seeded in hybridoma cloning plates (Greiner, Germany) in alpha minus MEM supplemented with 10, 25, or 50 ng/ml of methotrexate plus 1 mg/ml G418. After about 10-14 days single clones are trypsinized and then seeded in 6-well petri dishes or 10 ml flasks using different concentrations of methotrexate (50 nM, 100 nM, 200 nM, 400 nM, 800 nM). Clones growing at the highest concentrations of methotrexate are then transferred to new 6-well plates containing even higher concentrations ofmethotrexate (1 tpM, 2 j.M, 5 piM, 10 mM, 20 mM). The same :procedure is repeated until clones are obtained which grow at a concentration of 15 100-200 jpM. Expression of the desired gene product is analyzed, for instance, by SDS-PAGE and Western blot or by reversed phase HPLC analysis.

Example 5: Quantitative Murine Soft Tissue Infection Model for E. faecalis Compositions of the present invention, including polypeptides and peptides, are assayed for their ability to function as vaccines or to enhance/stimulate an immune response to a bacterial species E. faecalis) using the following quantitative murine soft tissue infection model. Mice NIH Swiss female mice, approximately 7 weeks old) are first treated with a biologically protective effective amount, or immune enhancing/stimulating effective amount of a composition of the present invention using methods known in the art, such as those discussed above. See,e.g., Harlow et al., ANTIBODIES: A LABORATORY MANUAL, (Cold Spring Harbor Laboratory Press, 2nd ed. 1988). An example of an appropriate starting dose is per animal.

The desired bacterial species used to challenge the mice, such as E. faecalis, is grown as an overnight culture. The culture is diluted to a concentration of 5 X 108 cfu/ml, in an appropriate media, mixed well, serially diluted, and titered. The desired doses are further diliuted 1:2 with sterilized Cytodex 3 microcarrier beads preswollen in sterile PBS (3g/100ml). Mice are anesthetize briefly until docile, but still mobile and injected with 0.2 ml of the Cytodex 3 bead/bacterial mixture into each animal subcutaneously in the inguinal region. After four days, counting the day of injection as day one, mice are sacrificed and the contents of the abscess is excised and placed in a 15 ml conical tube containing 1.0ml of sterile PBS. The contents of the abscess is then enzymatically treated and plated as follows.

The abscess is first disrupted by vortexing with sterilized glass beads placed in the tubes. 3.0mls of prepared enzyme mixture (1.0ml Collagenase D (4.0 mg/ml), 1.0ml Trypsin (6.0 mg/mi) and 8.0 mis PBS) is then added to each tube followed by a 20 min. incubation at 37C. The solution is then centrifuged and the supernatant drawn off. 0.5 ml dH20 is then added and the tubes are vortexed and then incubated for 10 min. at room temperature. 0.5 ml media is then added and samples are serially diluted and plated onto agar plates, and grown overnight at 37C. Plates with distinct oo and separate colonies are then counted, compared to positive and negative control samples, and quantified. The method can be used to identify composition and 20 determine appropriate and effective doses for humans and other animals by comparing the effective doses of compositions of the present invention with compositions known in the art to be effective in both mice and humans. Doses for the effective treatment of humans and other animals, using compositions of the present invention, are extrapolated using the data from the above experiments of mice. It is appreciated that further studies in humans and other animals may be needed to determine the most effective doses using methods of clinical practice known in the art.

Example 6: Murine Systemic Neutropenic Model for E. faecalis Infection Compositions of the present invention, including polypeptides and peptides, are assayed for their ability to function as vaccines or to enhance/stimulate an immune response to a bacterial species E.faecalis) using the following qualitative murine systemic neutropenic model. Mice NIH Swiss female mice, approximately 7 weeks old) are first treated with a biologically protective effective amount, or immune enhancing/stimulating effective amount of a composition of the present invention using methods known in the art, such as those discussed above. See,e.g., Harlow et al., ANTIBODIES: A LABORATORY MANUAL, (Cold Spring Harbor Laboratory Press, 2nd ed. 1988). An example of an appropriate starting dose is 20ug per animal.

Mice are then injected with 250 300 mg/kg cyclophosphamide intraperitonially.

Counting the day of C.P. injection as day one, the mice are left untreated for 5 days to begin recovery of PMNL'S.

The desired bacterial species used to challenge the mice, such as E. faecalis, is grown as an overnight culture. The culture is diluted to a concentration of 5 X 15 cfu/ml, in an appropriate media, mixed well, serially diluted, and titered. The desired doses are further diliuted 1:2 in 4% Brewer's yeast in media.

Mice are injected with the bacteria/brewer's yeast challenge intraperitonially. The Brewer's yeast solution alone is used as a control. The mice are then monitered twice daily for the first week following challenge, and once a day for the next week to ascertain morbidity and mortality. Mice remaining at the end of the experiment are sacrificed. The method can be used to identify compositions and determine appropriate and effective doses for humans and other animals by comparing the effective doses of compositions of the present invention with compositions known in the art to be effective in both mice and humans. Doses for the effective treatment of humans and other animals, using compositions of the present invention, are extrapolated using the data from the above experiments of mice. It is appreciated that further studies in humans and other animals may be needed to determine the most effective doses using methods of clinical practice known in the art.

The disclosure of all publications (including patents, patent applications, journal articles, laboratory manuals, books, or other documents) cited herein are hereby incorporated by reference in their entireties.

The present invention is not to be limited in scope by the specific embodiments described herein, which are intended as single illustrations of individual aspects of the invention. Functionally equivalent methods and components are within the scope of the invention, in addition to those shown and described herein and will become apparant to those skilled in the art from the foregoing description and accompanying drawings. Such modifications are intended to fall within the scope of the appended claims.

Throughout the specification, unless the context requires otherwise, the word "comprise" or variations such as "comprises" or "comprising", will be understood to imply the inclusion of a stated integer or group of integers but not the exclusion of any other integer or group of integers.

Page(s)2$?7-3O are claims pages they appear after the sequenc ~sting TABLE 1. Nucleotide and Amniho Acid Seqeuences of E. faecalis Genes.

EFOO1-1 (SEQ ID NO:l)

TGAAAGAATA

AAAAAAATGA

TTGGGGGCTT

AAGGAAGATA

ACAAAATTAA

CAATCTGCTT

TTACCAACAA

TTAGTGGACT

ATTCGTGAAG

AATAAATCGA

GTACCGAAAA

AAAGAAGTCG

AACAAAGGCG

GTGGACTATT

TATTTATCTG

GCTGG ITITG

CCTGAAAAAA

GAACAACGGA

TACTGGGCAC

TACAAAAATT

TTCGCTATCC

GAAAGTATTT

CAATTTGA.AC

TTGCCAGAAC

AGTTCAAAAC

GTGGTAACGG

CCACAATCAC

CGAAAACT

ACCCTGATT

P1'ACGCAACC

TAAAACCATA

TATTGTTAGA

CAGAAATGTT

CATTAGAGGA

'ITGGTGCTGG

'PTGATTAA

ACCGTGATGG

GCCCATTTC

TTCAAAAAGA

TCAACTTACA

CAGCGGCATT

AACAAACAGG

CTAAGACAAC

CAGTAGAAGA

TTGCTTCATC

AAGCATGGAA

GTGGCGAGCA

TCTAGCAACA

TAATGGGGCC

TTCTGGCAT

CATGAAAG2A

ACAAGCCAAA

GTACCCAGGC

TATGGATGAT

CGGCGCCAAA

ATTCTATAAT

A ITAAAAGAA

TT=ACTCG

TAAAGACTTA

TATCGAAGCA

AAACAAAAAG

TGCTGAAGCT

ACAAGGAACA

TGAATTCATG

TTATATGCCA

CAALAGTACCT

AAATGCTGAT

AAATAAAGAC

CCAATAA

AATTrGTITTA

ACAGTGTTAG

AA.AGAATCAA

GCAATG-AATG

AATCCAAAAA

ATCAATTCGA

TGGTTATOGA

GACACAATCG

ATCGACGGCA

GGTETTGT

CCTTCTAAAA

'rrAAATAACT

GATTAACAA

GGTTACTTCC

GTAGCAATGT

GGTGGCTATG

GATATTATA

AAATTCTTAG

ATFIT17AGAAT

GCACAACTTG

TCACCCTATA

ACGAGAAAAT

TAAATTTTT

CAACCGCAGC

ACGATATTGT

GGGTTCAAGA

?I'AAAGTGGA

CTTAACTTC

ATGCTGCACA

GCTGGAAAGA

AACPLATACGG

TGAAAGAATA

CAATTTACCA

ATTACGPA.AT

GCAAAGATTC

GCACAGCT-GG

TTGTCGGTAG

AATACGGTGT

TC'ITCGATAG

CTACTCCTGA

CTGTTTTACA

AAAACGCAGT

ATrGAAATGCG

TATTGAAAGA

TAAGGGAGAG

TATTFCGCA

GAAAGAAGTG

AGAAGCGTTA

AFI'ACAAAAT

ACCAAAAGAT

AGATGAAATG

TGCCAGCCA

CATTCCATTT

TrGGTGTTGAA

AAAATCCAAC

TGGAATGAAA

ACAAGAAGTC

TTCAGATAAA

TATTGCTGGT

TGCACCACGT

TGCTACGCCA

TI'CACAATTG

CAGTGATGAG

AAAAGATTTA

GACAATTATG;

TGCAACATCA

EF001-2 (SEQ ID NO:2) MKFRTLATT VLATAAIFAL GACGNGNGAK ESNDIVKEVK

S.

EDTTITFWHA

PTITQAYPGW

KSTEMLFYNA

KGVDFNKDLD

GFVQKDAEAG

WAQQTGYMP I S IFASSNKDT

MNGVQEEALT

LWNAAQD04L

DLIJKEYGVEV

LTSKDSQEVV

GYEYGVAPRP

LESVLHSDEY

RKr 4

LKDATSQ

KLTKDFMKEN

VDLKPYMDDD

PKTLEELKEA

DYYRDGIEAG

EK INLQQGTD

KNSKTTKVPA

FEQAWNQ

PKIKVELQNQ

TIGWKDAEPI

SKTIYEKSNK

YFRTAGSDKY

IYMFDSATPE

QL.ENAVKDLF

SAYPDLQAKI

REVLLDGARI

EVVGAGFD-SL

LSGPFANKKV

QRTAAFEFMKfl

AIPVEENADS

NSTLTSPKDL

DGKQYGIPFN

NNYYAIGMKN

ANFVGS IAGA

FLATPOSQLY

AYNEMRTIME

EF001-3 (SEQ ID NO:3) 7T GTCCTAACGG TAATGGGGCC AAAGAATCAA ACG-ATMrrGT GAAAG.AAGTG

AAGGAAGATA

ACAAAATTAA

CAATCTGCTT

TTACCAACAA

TTAGMGACT

ATTCGTGAAG

AATAAATCGA

GTACCGAAAA

AAAGAAGTCG

AACAAAGGCG

GTGGACTATI'

TATTATCTG

CGACAATCAC

CGAA.AGACTT

ACCCTCA ITT

TTACGCAAGC

TAAAACCATA

TATTGTTAGA

CAGAAATGTT

CATTAGAGGA

TTGGTGCTGG

TTGATTTAA

ACCGTGATGG

CCCCATTTGC

TTCTGGCAT

CATGAAAGAA

ACAAGCCAAA

GTACCCAGGC

TATGGATGAT

CGGCGCCAAA

ATTCTATAAT

ATTAAAAGAA

TTTTGACTCG

TAAAGACTTA

TATCGAAGCA

AAACAAAAAG

GCAATGAATG

AATCCAAAAA

ATCAATTCGA

TGGTI'ATIGGA

GACACAATCG

ATCGACGGCA

GCTGATrTGT

GCTI'CTAAMA

ITrAAATAACT

GATTTAACAA

GGTTAC'FrCC

GTACCA.ATGT

GGGTTCAAGA AGAAGCGTITA TTAAAGTGGA ATTACAAAAT CITAACTTC ACCAAAAGAT ATGC~f-CACA AGATGAAATG GCTGGAAAGA TGC-AGAGCCA AACAATACGG CATTCCATTT TGAAAGAATA TGGTGTTGAA CAATTIACGA AAAATCCAAC ATTACCCAAT TGGAATGAAA GCAAAGATTC ACAAGAAGTC GCACAGCTGG TTCAGATAAA ?I'GTCGGTAG TATTGCTGGT TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

GCTGGCTTTG

CCTGAAAAAA

GAACAACGGA

TACTGGGCAC

TACAAAAATT

TTCGCTATCC

GAAAGTATTT

CAATTTGAAC

TTCAAAAAGA

TCAACTTACA

CAGCGGCATT

AACAAACAGG

CTAAGACAAC

CAGTAGAAGA

TTGCTTCATC

AAGCATGGAA

TGCTGAAGCT

ACAAGGAACA

TGAATTCATG

TTATATGCCA

CAAAGTACCT

AAATGCTGAT

AAATAAACAC

CCAA

CGTCGCTATC

GATATTTATA

AAATTCTTAG

ATTTAGAAT

GCACAACTIG

TCAGCCTATA

ACGAGAAAAT

AATACGGTGT

TGTTCGATAG

CTACTCCTGA

CTGTrrTACA

A-AAACGCAGT

ATGAAAT'GCG

TATTGAAAGA

TGCACCACGT

TGCTACGCCA

rrCAAATTG

CAGTGATGAG

AAAAGATrITA

GACAATTATG

TGCAACATCA

EF001-4 (SEQ ID NO:4) CGNGNGAX< ESNDIVKEVK EDTTITFWHA MNGVQEEALT PTITQAYPGW LWNAAQDEML KST2EMLFYNA DEJLKEYGVEV KGVDFNKDLD LTSKCSQEVV GFVQKDAEAG GYEYGVAPRP WAQQTGYMP I LESVLHSDEY **SIFASSNXDT RKLLKDATSQ

KLTKDFMK~EN

VDLKPYMDDD

PKTLEELKEA

DYYRDGIEAG

EKINLQQGTD

KNSKFI'KVPA

FEQAWNQ

PK IKVELQNQ

TIGWKDAEPI

SKTIYEKSNK

YFRTAGSDKY

IYMFDSATPE

QLENAVKDLF

SAYPI)LQAKI

REVLLDGAKI

EVVGAGFDSL

LSGPFANKKV

QRTAAFEFMfl Al PVEENADS

NSTLTSPKDL

DGKQYGIPFN

~'NYAIGbM(N AMFVGS EAGA

FLATPDSQLY

AYNEMRTIME

*5

S

S.

S S

S

S.

S S

S

SSSSS

S

5*5S

S

5.5.

S

*555

S

S S *5 EF002-1 (SEQ ID

TAAATAGCGG

TTAGCAGTGG

GAAAAGAGTG

CCAGAATTTG

CCGGTGGACA

GATACGACGG

AATCAATTGG

AGTTACGAGA

TGGGTATTGT

TTAACTTGGG

TATGGTGCCT

AATGCCAATT

AGAATGCAAA

TATCAATCAC

GGGACTTTAT

ATACCACAAC

AATAAAAACA

CAAGGTCCAA

GATAAAATCT

AGGTAGTACA

CGGCACTAAC

AAGATGGCAA

AGAAATTATT

TTGCTTCAGA

ATATTTTAAC

TGGATTTAAC

TGTATGAAAT

ATTACAATAA

ATGAATATGA

ATCAACATAC

TCATI'GAACC

AAGATCAATC

AATTTGAAAA

TAACAAACAT

AAGAAAAAGG

GTAAAAAACA

AACTT1rAGC

ACTTTGCAAC

AATGAAAT'Fr

TTTAACAGCA

AACAAAATTA

CAGAGCTTTT

TGATTATGAC

CATGAAAAAC

CGATCACG LT

CGATGGTAAA

AAAAATCTTT

AGCGTTAGCG

TTGGCGCTCA

AAAATACAAT

ACAAATGGAT

TTCAAA.AGCG

TCATrGATGGC

CAAAGCAACT

AAAAGCTGCT

AGAAGTAGGG

AAAAGGAATG

TGGAAAAAAG

TGTGGTGGTT

ACAGTAACTA

GAAGCGGAAA

ACAAAAGTAA

TTAC=ICAT

AAAGATTTAG

ACCTATGCTC

CATGAAGCCG

AAAAAATTAT

ACCGTTCAAG

TATATGGAAA

TTTGGAACAG

GCGATGATGT

AAAACAAATG

ACCTTTGGCT

CAAAAA'FrCT

GTGGTTCCTT

CCTTCAGACG

GCTTAACAGC

CAAGTGAAAA

CI-TGGAATTA

ATCCTGATAT

CAACGATGCT

'ATTCTA-ATTA

ATATCGAACC

AGCCTTACCG

CAATTGCCTA

CTAAACCACA

CGATI'GCTGC

CTTATTATGA

CAA-AATCAAC

ACATGGGTAG

TCGAATGCGG

CACCGACAAG

TAGACTTTC

CTTATAAAAC

AGTCTCACAA

GGCAGCGCTG

GAAACCAACT

TGACACGACC

CACTATTIAA

rrCATCAGGA

CGCGCTACGC

TGCCAAAGCA

TACAGA ITC

TCCCGATAAC

AGAACAAGTA

TGCTCAAAAC

TCGCGCATTG

AAAAGTAACG

CTGGTACATG

GATTGCCGAA

TTTTGCAATT

TTCAGGTAAA

AGATGAAATT

AAAGCCTTTA

ACCCAGATAC AATTAATTTA G EF002-2 (SEQ ID NO:6) MKFW KXGLTAAALL AVAAVTLTAC GGSSEKKATE KSEDGKTKLT Vr7IMTYDP EFEKLFRAFE AENPDITIEP VDIASDDYDT KVTT~1LSSGD TITD ILTMKNL LSYSNYAIJRN QLVDLTDHVK DLDIEPAKAS YEMYEIDGKT YAQPYRTDFW VLYYNKKMFD EAGIAYPDNL TWDEYFEALAX KLSKPEEQVY GAYQHTWRST VQAIAAAQNN ANLIEPKYNY METYYDRALR MQKDQSQMDF GTAKSTKVTY QSQFENSKAA MMYMGSWYMG TLLTNIDDGK TNVEWGIAEI PQQEKGYKATT FGSPTSFAIN KNSKXQKAAQ KFLDFASGKE GAXLLAEVGV VPSYKTDEID 81 TABLE 1. Nucleotide and Amnino Acid Seqeuences of E. faecalis Genes.

KIYFARKGMP SDESHflKLT QIQLI EF002-3 (SEQ ID NO:7) A TGTGGTGGTT CAAGTGAAAA GA-AAGCAAC GAAAAGAGTG AAGAT.3GCAA AACAAAATTA

CCAGAATTTO

CCGGTGGACA

GATACGACGG

AATCAATTGG

AGTTACGAGA

TG'-TATTGT

TTAACTTGGG

TATGGTGCCT

AATGCCAATI'

AGAATGCAAA

TATCAATCAC

GGGACTTTAT

ATACCACAAC

AATAAAAACA

GAAGGTGCAA

GATAAAATCT

ACCCAGATAC

AGAAATTATT

TTCCTTCAGA

ATAT=AAC

TGGXFFI'AAC

TGTATGAAAT

ATTACAATAA

ATGAATATGA

ATCAACATAC

TGATTGAACC

AAGATCAATC

AATTTGAAAA

TAACAAACAT

AAGAAAAAGG

GTAAAAAACA

AACTTTTAGC

ACTTIrGCAAG

AATTAATT

CAGAGCTT

TGATTATGAC

CATGAAAAAC

CGATCACGTT

CGATGGTAA

AAAAATGTTT

AGCGTTAGCG

TTGGCGCTCA

AAAATACAAT

ACAAATGGAT

TTCAAAAGCG

TGATGATGC

CAAAGCAACT

AAAAGCTGCT

AGAAGTAGGG

AAAAGGAATG

T

ACAGTA-ACTA

GAAGCGGAAA

ACAAAAGTAA

TTACTTCAT

AALAGAT'IAG

ACCTATGCTC

CATGAAGCCG

A.AAAAATTAT

ACCGTTCAAG

TATATLGGAAA

T=~GAACAG

GCGATGATGT

AAAACAAATG

ACCTFIGCT

CAAAAATTFCT

GTGCFTCCTT

CCTTCAGACG

CTGGAATTA

ATCCTGATAT

CAACGATGCT

ATTCTAATTA

ATATCGA.ACC

AGCCTTACCG

GAATTGCCTA

CTAAACCAGA

CGAT GC C~rAkTTATGA

CAAAATCAAC

ACATGGGTAG

TCGAATGGCG

CACCGACAAG

TAGACTTTGC

C'ITATAAAAC

AGTCTCACAA

TGACACGACC

CACTAITGAA

TTCATCAGGA

CGCGCTACGC

TGCCAAAGCA

TACAGATTTC

TCCCGATAAC

AGAACAAGTA

TGCTCAAAAC

TCGCGCATTG

AAAAGTAACG

CTGGTACATG

GATTGCCGAA

=TTGCAATT

TTCAGGTAAA

AGATGAAATT

AAAGCCTTTA

S

EF002-4 (SEQ ID NO:8) C GGSSEKKATE KSEDGKTKLT VTrrdNYDTTP EFEKLFRAFE AENPDITIEP VDIASDDYDT KVTTKLSSGD QLVDLTDHVK OLDIEPAKAS YEMYEIDGKT YAQPYRTDFW TWDEYEALAK KLSKPEEQVY GAYQHTWRST VQAIAAAQNN MQKDQSQMDF GTAKSTKVTY QSQFENSKAA MMYMGSWYMG PQQEKGKATT FGSPTSFAIN KNSKKQKAAQ KFLDFASGKE KIYFARKGMP SDESHKKPLT QIQLI TTD ILThKNL

VLYYNKKMFD

ANL IEP(YNY TLLTMI DDGK

GAI<LLAEVGV

LSYSNYALRN

E-AGIAYPDNL

METYYDRALR

TNVEWGIAEI

VPSYKTOEID

EF003-1. (SEQ ID NO:9) TAGGAGGACA A.AAGAATGAA ATITAGCTG CCTGTGGGGG GTTGCCGTGC AATTGGAATC AAAA.AAGGGT ACAAAATTAA GTGCAACATG ACGAAGCGGA AACAAAGAGA AAAAAGCTGA TTCTATTCAA AAGAATACCA CCTAGCGATC CAACCAATGA AAATTAAAAG AAGGTGTCGG AACATCACIT TTGAAAGCAT ATCGCTATGG TGTTCTGCTA GCGATCTITGT TAGAAGATAA AAAGGCGAAA AAGATAGCGA G'ITGCTGAAT ACATCAAGAA GAAGTT71TAT

AAATAAACAA

TTCAAAAGAT

CATTATGGAA

TGCTAATT

TTTAGTGGCT

AGATGCGAAA

AGGTCGTGCT

CTTAACGGC

TGATTTACTG

CCCAGCCTAC

AGAAGCAAGT

AAAAATcAAr,

AAATTCTAAA

TTAGCNACAT TCGCTGTrAT TGCAACAGTT

GCAGACCAGA

ATCTTGGAGA

GTGAGCGACA

GCGCAACATC

GTGCAACCGA

GATTrACCTG

TTAGCAA=

ACGGTGGCAG

AATI'AGCTA

TTAGAACCTG

AAACATTACG

GTTTTAAAAG

GGCGCCAATA

AAGAAGACAA

TTGCCAAGAA

ATGTTGCCTA

AACCCTTAT

TTTATTATT

AAAATGCCAA

TAA.ATGCAAA

ATGTCGTGGA

AAGCCTATGA

CTGGTTAAC

cATTGCAAGT

AAGCGATGAC

TTCCTGCGT

GGAGATTACC

AGAAGCTGAG

CAACGATGCC

GGAAATGTT

TGCTGGTGGT

AGTO-GGGATT

CGGCGTGATT

AAATCCTAAA

TCAAAALAGAC

AACGAALAGAT

TGTGACACGC

AACAAAAGAA

TTAA

EF003-2 (SEQ ID NO:1O) 82 TABLE 1. Nucleotide and Amino Acid Seqcences of E. faecalis Genes.

MKKFYL ATFAVIATVI LAACGCNXQA DQKEDKEITV AVQLESSKD)I LEIAXXEAEI( KGYKINIMEV SDNVAYNflAV QHDEADANFA QHQPFMEMFN KEKKADLVAV QPIYYFAGGF YSKEYQDAKD LPENAKVGIP SOPTNEGRAL AILNANGVIK LKEGVGFNGT VADVVENPKN ITFESIDLLN L.AKAYDEKDI AMVFCYPAYL EPAGLTTKDA ILLEDKEASK HYALQVVTRK GEKDSEKIK'J LKEAM~TTKEV AEYIKKNSKG ANIPAF EF003-3 (SEQ ID NO:11) CTGTGGGGG AA.ATAAACAA GCAGACCAGA AAGAAGACAA GGAGATIACC

GTTGCCGTGC

AAAAAAGGGT

GTGCAACATG

AACAAAGAGA

TTCTATTCAA

CCTAGCGATC

AAATTAAAAG

AACATCACTT

ATCGCTATGG

GCGATCTTGT

AAAGGCGAAA

GTTGCTGAAT

AATI'GGAATC

ACAAAATTAA

ACGAAGCGGA

AAAAAGCTGA

AAGAATACCA

CAACCAATGA

AAGGTGTCGG

TTGAAAGCAT

TGTTCTGCTA

TAGAAGATAA

AAGATAGCGA

ACATCAAGAA

'TCAAAAGAT

CATTATGGAA

TGCTAATTT

TI'TAGTGGCT

AGATGCGAAA

AGGTCGTGCT

CFI'TAACGGC

TGATTTACTG

CCCAGCCTAC

AGAAGCAAGT

AAAAATCAAG

AAATTCTA.A

ATCTTGGAGA

CTGAGCGACA

6CGCAACATC

GTGCAACCGA

GATTTACCTG

TTAGCAATT

ACGGTGGCAG

AATTIAGCTA

TTAGAACCTG

AAACATTACG

GTTTTAAALAG

GGCGCCAATA

TTGCCAAGAA AGAAGCTGAG ATrGTTGCCTA CAACGATGCC AACCCTTCAT GGAAATGITT T'ITATTATTT TGCTGGTGGT AAA.AltCCAA AGTGGGATT1 TAAATGCAAA CGGCGTGAT ATGTCGTGGA AAATCCTAAA AAGCCTATGA TCAAAAAGAC CTiGGTTrAAC AACGAAAGAT CATTGCAAGT TGTGACACGC AAGCGATGAC AACAAAAGAA TTCCTGCGTT T

S*

S

S.

S

.5

S

S

S

EF003-4 (SEQ ID NO:12) CGGNKQA DQKEDKEITV AVQL.ESSKDI LEIAKKEAE-K KGYKINIMEV SDNVAYNDAV QH.DFADANFA QHQPFMEMFN KEKKADLVAV QPIYYFAGGF YSKEYQDAXD LPENAKVGIP SDPTNEGRAL AILNANGVIX LKEGVGFNGT VADVVENPKN ITFESIDLLN LAKAYDEXDI AMVFCYPAYL EPAGLTTKDA ILLEDKEASK HYALQVVTRK GEKDSEKIKV LKEAMTTKEV AEYIKKNSKG ANIPAF EF004-1 (SEQ ID NO:13)

TAAATCGAAA

ATTIrGCAG

GAAACTG.CTG

ACAGAAAACA

TTAGTAAAAA

GTCATTACGG

GCGATAGAT'

AATGTTGAAG

CCTTACACGA

GAAGGATGAT

CTCTICTCTC

CCCAGTCAAG

TAACCCAAGC

TAGATGIrAA 7rAAGGTAAT

CCAACTCAGG

AAGTAGCCAA

ATGGGA-ACGA

AGAAATGAAA

TGCCTCTAC

CACTA'FrGAA

AGTTAAACAG

AAATAATGTT

TAATCATGAA

TACAGAGGCA

AACATTAGAA

CAGAACCATA

AAAATGATTA

AACGCAAAAA

GCTTrCAGACA

TTAGAAGAAA

AAAGATGACA

GCAAAAAAAA

CAAAAGACTG

AATGATAACC

GCAAAATCAA

AATTTGCAGG CATTGCTCTT ATAATACACA AALAGAAAG"C GTAACGAAAA CGAGCCTAAT AATTTAACTC TGACGAGAAA CATCAGATAA CCCTCACGCT ATATGGAACA AATGCAGACT CCATATACGG AATCAATTA ATGTTATTTC TTrCATCACA CTAAAAATGA AAATA TTATT CCGTTAGTAA AATAA EF004-2 (SEQ ID NO:14) MKK MIKFAGIALI FAALLSACSN AKNNTQKKAE TAAQSSTIEA SDSNENEPNT ENITQAVKQL EE-KFNSDEXL VKIDVKNNVK DDTSDNPIIAV I'I' VINDEA KKNMEEMQTA IDSNSGTEAQ KTAIYGIQLN VEEVAKTLEN DNDVISFITP YTNCNDRTIA KSTKNENIIP

LVK

EF004-3 (SEQ ID 83 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Gcncs.

CTGTAGC AACGCAAAA.A ATAATACACA AAAGAAAGCC

GAAACTGCTG

ACAGAAAACA

ETAGTAAAAA

GTCATTACGG

GCGATAGAT

AATGTTGAAG

CCTTACACGA

CCCAGTCAAG

TAACCCAAGC

TAGATGTI'AA

TTAAGGTAAT

CCAACTCAGG

AAGTAGCCAA

ATGGGAACGA

CACTATI'GAA

AG'ITAAACAG

AAATAATGT

TAXTGATGAA

TACAGAGGCA

AACATTAGAA

CAGAACCATA

GCTTCAGACA

TAGAAGAAA

AAAGATGACA

GCAAAAAAAA

CAAAAGACTG

AATGATAACG

GCAAAATCAA

GTAACGAAA

AATTTAACTC

CATCAGATA.A

ATATGGAAGA

CCATATACGG

ATGTTATTTC

CTA-XAAATGA

CGAGCCTAAT

TGACGAGAAA

CCCTCACGCT

AATGCAGACT

AATTCAAMTA

TTTCATCACA

AAATATTATT

CCGTI'AGTAA AA EF004-4 (SEQ ID NO:16) CSN AXNNTQKXAE TAAQSSTIEA SDSNENEPNT ENITQAVKQL~ EEKFNSDEKL VKIDVKNNVK DDTSDNPHAV TTVKVINDEA KKNNE7EMQTA IDSNSGTEAQ KTAIYGIQLN VEEVAKTLEN DNDVISFITP YTNGNDRTIA KSTKNENIIP

LVK

EF005-1 (SEQ ID NO:17) Not* *000 0* 060 S

TAAAAAATGA

GTTGGTTGtG

CTAACTCTTT

GGATGGTCAG

GGACTGAAAG

ACTGCTCAAC

CCAGATTAC

CAGGATATTG

GAATCCT LTG

TGGCCTGCAG

GTTG.CCACAG

AGTATTI'CAG

A.AGAATGCTA

AAAAACGATT

GTAAAAATCA

ACATTGTGCG

ATGCGGTCCT

ATGTTGCCTT

TTA T=AGA GTGAATT1'AA

CTGATGATCA

CCAATAGTGT

AAGACTATGC

AATCAGCCAA

CGTTGTLTAGC

GTGTCACAAC

CACGATTGTG

GCAAGCAACG

CCACGGAAAA

AACACCAGAA

TCAAAATGCA

TCAAAATAAA

TTTGGTAGC

AGGTGTTCC

AGCTAAACTG

TACAATI'ACT

TCTGGAAT

AACTTTATI

AATTCATTAC

AGCACGCGAA

GGGATGCTTT

ACAAAAGAAA

ACCATGTTAA

GGTGAAAAAG

TATAGTAGTG

GCAGGCAAAG

TATGAAGGGG

TTAGAAGAAT

GATCAACAGC

AAACGTTTGA

GGCAATGTTT

GATGATI'TA

AAAAATGGCG

AAAGAATCAA

TTCTGGCCAT

A.AGAGACAAA

ATACGACGGA

TTGTGACAGC

ATAGTGGCCG

ACCTTGAAGT

ATTTAAATAA

TTATGAAAAA

GCGAGGAAAG

AAAA.AGGCTr

TAGTGGTCTC

A.AGTCCCAGA

AATATAC=II

AATA.A

TTTAGTAATC

ACCTGAAGAA

CCGCGTACAA

AACTGGGAT

CGCCTTGCAA

CGTGCGTGAC

GACAATGTGG

CATGACTCCT

CAAGAATAAC

AGATAAAATT

TCATGGCTTG

AGGCGGTTTG

GGATAAAGTC

AATGATGTCA GCTACTTAGA EF005-2 (SEQ ID NO:18) MKKRLTIVG MLFLAILVMV GCGKNQQATT KEKETKPEEL ThY IVRHGKT MLN'rTDRVQG WSDAVLTPEG EKVVTATGIG LKDVAFQNAY SSDSGRALQT AQLILDQNKA GKDLEVVRDP DLREFNFGSY EGDLNKTMWQ DIADUQGVSL EEFfrflNMTPE SFANSVAKLD QQREESKNNW PAEDYATITK RLKKGLDKIV ATESANSGNG NVLWVSHGLS I SALLATLFD DFKVPEGGLK NASVTTIHYK NGEYTLDKVN DVSYLEAGEK ESK EF005-3 (SEQ ID NO:19) TTGTG GTAAAAATCA GCAAGCAACG ACAAAAGAAA AAGAGACAAA ACCTGAAGAA CTAACTCTT ACATTGTGCG CCACGGAAAA ACCATGTTAA ATACGACGG.A CCGCGTACAA GGATGGTCAG ATGCGGTCCT AACACCAGAA GGTGAAAAAG TTGTGACAGC AACTGGGAT GGACTGAAAG ATGTTGCCTT TCAAAATGCA TATAGTAGTG ATAG;TGGCCG CGCCTTGCAA ACTCCTCAAC TTATITI'AGA TCAAAATAAA GCAGGCAAAG ACCETGAAGT CGTGCGTGAC CCAGA=TAC GCGAT AA TTTIGGTAGC TATGAAGGGG AtTTTAAATAA GACAATGTGG CAGGATATTG CTGATGATCA AGGTGTTTCC TTAGA.AGAAT TTATGAAAAA CATGACTCCT TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

GAATCCTTTG

TGGCCTGCAG

GTTGCCACAG

AGTATTTICAG

AAGAATGCTA

AATGAMTCA

CCAATAGTGT

AAGACTATGC

AATCAGCCAA

CGTTGTTAGC

GTGTCACA-AC

GCTACTTAGA

AGCTAACTG

TACAATTACT

I'rCTGGGAAT

AACT'ITATT

AATTCATTAC

AGCAGGCGAA

GATCAACAGC GCGACGAAAG AAACGTTTGA AAA-AAGGCTT GGCAATGTTT TAGI'GCTCTC GATGATTTTA AAGTCCCAGA AAAAATGGCG AATATACTTT AAAGAATCAA AA

CAAGAATAAC

AGATAAAATT

TCATGGCTTG

AGGCGGTTTG

GGATAAAGTC

EF005-4 (SEQ ID CGKNQQATT KEKETKPEEL TLY IVRHGKT MLNTTDRVQG WSOAVLTPEG EKVVTATGIG LKDVAFQNAY SSDSGRALQT AQLILDQNKM GKDLEVVRDP DLREFNFGSY EGDLNKTMWQ DIADDQG'JSL EiE-FMKNMTPE SPANSVAKLD QQREESKNNWA PAEDYATITK RLKKGLDKIV ATESANSGNG NVLVVSHGLS ISALLATLFD DFKVPEGGLR NASVTTIHYK NGEYTLDKVN DVSYLEAGEK ESI( EF006-1 (SEQ ID NO:21) @0 0O S 0E S S

S.

0@ 0O S 0@

S.

S S 0

SO

S S 0 0 505.5.

S

*OS.

0 *050 *0 00@0 0 0000

S

0 *605 60 0 0*

S.

TAAACGATAA

GCAGTAGCTG

AAAG ETGGAG

GAAAAAGAAG

GCGTTGGAAA

GCGGTTAAAG

GGGC'ITACT

GTTAGCTCTT

ATCACGCTGA

ACTAAAAAGT

AATGAAGAAG

CCGAA.AAAAG

GTTCGTAA.AG

AAAGAAGTCC

ATGGAGGGAA

TCTTAGTTT

CTTCACCAGT

GCGTAAAATT

GTGGCGATAT

AAAATGATTA

CGAAAAAATA

CCGTTTCAGA

AAGAAGGGGT

TGAAATTCA-A

GGGCTGCGGT

ATGCGATTGC

AAGACGAAAA

AAGATTGGAT

TAAGATGAA

AGGGGCTTGC

TCCACATGCA

AGAAGTGACG

CGATCCCAAC

TGACTTTGTG,

CAAATCGTTA

TGGCCACGC

AGACCGGACA

TCATGAAAGT

TTTAArrAAC

CTTAGAAAAA

CAACGAAAAT

TACGAAAAAA

AAACGTACAT

GGCAATAA.AA

GAGA 1TTTAG

ACTTATACAG

TATFI'CCAAC

AATGCAGCGTG

CAAGAAATTC:

GTATTAACTA

ACTGCTACTT

GATCCAGC-AA

TCAAACTTTG

CAAAGTTCAC

GTAAAAAAAT

TGGAACGGCG

TATGC-TCAGT

AGAGTGATGA

A.ACATGTAAA

ATTACGTGCT

ATGTOCCGTT

CGATTCAT

CTGATGGTTC

TCTAGAAGA

TCGATGATAT

TCATGSACCAC

CCGTGGATCA

CTTATGCCAA

TAGTCAAAGT

CTATTGTECC

AATI'ACTGTA

CTCGGTCTTG

ACCTTTATTA

ACCTAACAAG

CTTTAATGAA

AGAACCAGTT

AACGATTTAC

TGCTGGTTTA

TGATAAAAAT

TCTTTATCAC

AGGTAAAT

TATTATTGCO

GTTACGTAC

AGTCAATGAA

TAA

EF006-2 (SEQ ID NO:22) MIKK RTLWSVITVA VAVLVLGACG NXKSDDSVLK VGASPVPH~AE ILEHV'KPLLE KEGVKLEVTT YTDYVLPNKA LESGDIDANY FQHVPFFNE-A VKENDYDFVN AGAIHLEPVG LYSKKYKSLQ EIPDGSTIYV SSSVSDWPRV LTILEDAGLI TLKEGVDRTT ATFDDIDKNT KKLKFNHESD PAIMT]TLYDN EEGAAVLINS NFAVDQGLNP KKDAIALEKE SSPYANI IAV RKEDE--NNENV KKLVKVLRSK EVQDWITKKW NGAIVPVNE EF006-3 (SEQ ID 1NO:23) TTGC GGCAATAAAA AGAGTGATGA CTCGGTCTTG

AA.AGTTGGAG

GAAAAAGAAG

GCGTTGGAAA

GCGGTTAAAG

GGGC ITTACT

GTTAGCTCT

ATCACGCTGA

ACTAAAAAGT

AATGAAGAAG

CTTCACCAGT

GCGTAAAATT

GTGGCGATAT

AAAATGATTA

CGA.AAAAATA

CCGTTTCAGA

AAGAAGGGGT

TGAAATTCAA

GGGCTGCGGT

TCCACATGCA

AGAAGTGACG

CCATGCCAAC

TGACTTTGTG

CAAATCGTTA

'rrGGCCACGC

AGACCGCACA

TCATGAAAGT

TTTAATTAAC

GAGXTTTAG

ACTTATACAG

TATTTCCAAC

AATGCAGGTC,

CAAGAAAVTC

GTATTAACTA

ACTGCTAC1'

GATCCAGCAA

TCAAACTT'rG

A.ACATGTAAA

ATTACGTGCT

ATGTGCCGTT

CGA'rTCAT'TT

C-MATGGTC

TCTAGAAGA

TCGATGATAT

TCATGACCAC

CCGTGGATCA

ACCTTTATTA

ACCTAACAAG

CTTTAATGAA

AGAACCACTT

AACGATTTAC

TGCTGGTTTA

TGATAAAAAT

TCITTATGAC

AGGATTAAAT

TABLE 1. Nucleotide and Amino Acid Seqeuences of E.]a~ecajlis Genes.

CCGAAAAAAG ATGCGATTGC CTTAGAAA.AA GAAAGTTCAC CTTATGCCAA TATTAT'TGCG GTTCGTAAAG AAGACGAAAA CAACGAAAAT GTAAAAAAAT TAGTCAAACT GTTACGTAGC AAAGAAGTCC AAGATTGGAT TACGAAAAAA TGGAACGGCG CTATTGTTCC AGTCAATGAA EF006-4 (SEQ ID NO:24) CG NKKSDDSVLK VGASPVPHAE ILEHVKPLLE

KEGVKLEVTT

LYSKKYKSLQ

KXLKFNHESD

RKEDENNENV

YTDYVLPNKA

El PDGSTIYV PAIM ETLYDN

KKLVKVLRSK

LESGDIDANY

SSSVSDWPRV

EEGAAVLINS

EVQDWITKKW

FQHVPFFNEA

LTI LEDAGL I

NFAVDQGLNP

biGAIVPVNE VKENOYDFVN AGAI HLEPVG TLKEGVDRTT ATFDDIDKNT KXDAIALEKE SSPYANI IAV EF008-1 (SEQ ID V00*0 0.00.

.0.0* 0 TA.AAC CGTGA

ACACTTTTAG

AA.ATTAGCAA

GACAAAATTG

TTACCAGAAG

TI'AGAAACAG

AATAAAGAT

CAAGAACAAA

CAAAACATTC

AACGCGAAAA

GCTGATATTC

TCCAAAGCTT

ACACCTGAAC

ITTTGTTIGAAA

CCAATTTACG

TACTACAGCA

GAAAGAAATG

CAGGGTTA-AC

TTGCIGACAAC

AGCTGCATAG

ACATTGCGAA

GCGGAAATG

ACTTTTCTAC

CAGAAGATCC

GTGACGTCTT

ATTATACCGA

CTGATGATAA

ATGATTTAAA

AAATGACCAC

CCAGTGTCGA

ATACACTTTT

TGATGAACTG

GAGGAATCAA

GTTAGCTGCT

GAACTCCATC

TATTGTGCCA

AGCTTCTGAA

CTGTTAAC

AAGCAAAAAT

ACATGCTTGG

AGTAGAAAAA

AAAACTTAGC

AAAATTATTA

TGCCGCTTAT

GATTATTGAT

TAAACGTAGT

CACAGACTCT

GAATTTAACA

CGAATGAAAA

TGCGGGAATC

CTATCTGATT

ATTGGGACAG

GCGGACATT=

AAA'rTAATGA

GTTACGCCAC

TAGACATTG

GATCCAAAAA

AAACTACATG

GTTACAAGTG

ATFI'GGGAAA

ACCATTAAGA

ATGGAACGGG

CTTGCCAAAG

AAAATCCATG

AATTTAGI I

AAGCCGCTGA

TAGTGAAAAA

ACCCTCACGA

TATTCTTI'AA

AAACGGCCAA

AATATT1'AAC AAAATGr.CAT ATAAAGATrT

AGGAAGCCAA

AAGGTGCCTT

.TTAACACAGA

AATCAAAAGC

TCTCAAAAGA

AAGGAACAWA

ATGGCTIAAT

ATITTTTTA

AAkAGAAAGAA

TGTTGGGCAA

ATATGAACCG

CGGCTTGAAC

AAAAGTTGAG

AAGTGCCGGT

TAAATATGTA

CTATACAGAA

AGCTAAXTTT

TAAATATTTC

AAGTCAAGGDN

ACCTGTGTTA

AGTGAAACGA

AGCGCATACG

CGTAAATAA

EF008-2 (SEQ ID NO:26)

M(KFSLFFLT

KIELHSIVPI

KDYFSTSKNV

AKNYTEKLSK

PEQMTTIIDT

YSIOUNWNLTK

LLAGLTEJAAC

GTDPHEYEPL

TPQYLTSAGQ

LHEEAYJAKFA

IKKSKAPVLF

IHDGLMSK

GNQAAEKKEK

PEDIAKASEA

EQTEDPHAWL

DIPDDKKLLV

VETS VDKRSM LAIVTTNS IL SDLVKNVGQD DI LFFNGLNL ETGGNGWFNK DIENGIKYVE NIRDVLVEKD TSEGAFKYFS KAYDLNAAYI ERVSKEVKRP IYDTLFTDSL

LW(TAK<KVEN

PIQNKDFYTEN

WEINTESQGT

AKEGTEGDY

EFOOS-3 (SEQ ID NO:27) T TCGGGAATC AAGCCGCTGA AAAGAAAGAA

AAATTAGCAA

GACAAAATTG

TTACCAGAAG

TTAGAAACAG

AATAAAGATT

CAAGAACAAA

CAAAACATTC

AACGCGAAAA

GCTGATA ETC

TI'GTGACAAC

AGCTCCATAG

ACATTGCGAA

GCGGAAATGG

ACITTTCTAC

CAGAAGATCC

GTGACGTGTT

ATTATACCCA

CTGATGATAA

GAACTCGATC

TATMTGCCA

AGCTTCT'GAA

CTCGTTTAAC

AAGCAAAAAT

ACATGCTTG4G

AGTAGAAAAA

AAAACTI'AC

AAAATTATTA

CTATCTGATT TAGTGAAA ATTGGACAG ACCCTCACGA GCGGACATTT TATTCTTTAA

AAATTAA'IGA

GTTACGCCAC

TTAGACATTG

GATCCAAAAA.

AAACTACATG

GTTACAAGTG

AAACGGCCAA

AATATT-A.AC

AAAATGGCAT

ATAAAGATIT

AGGAAGCCAA

AAGGTGCCTT

TGTTGGCAA

ATATGAACCG

CGGCTTGAAC

AAAAGTTGAG

AAGTCCCGGT

TAAATATGTA

CTATACAGAA

AGcTAAATT

TAAATATTTC

86 TABLE 1. Nucleotide and Am~ino Acid Seqeuences of Efaecalis Genes.

TCCAAAGCTT

ACACCTGAAC

TTTGTTGAAA

CCAATTTACG

TACTACACCA

ATGATTTAAA

AAATGACCAC

CCAGTGTCGA

ATACACITTTT

TGATCAACTG

TGCCGCTTAT

GA'LTATTGAT

TAAACGTAGT

CACAGACTCT

GAAT'IAACA

ATTTGGGAAA TTAACACAGA ACCATTAAGA AATCAAAAGC ATGGAACGGG TCTCAAAAGA CTTGCCAAAG3 AAGGAACAGA AAAATCCATC ATGGCTTAAT

AAGTCAAGGN

ACCTrCTGTTA

AGTGAAACCA

AGGCGATACG

GAGTAAA

EFOOS-4 (SEQ ID N0:28) C GNQAAEKKE( LAIVTTNS IL SOLVKNVGQD KIELHSTVPI GTDPHEYEPL PEDIAKASEA DILFFNGLNL KDYFSTSi(NV TPQYLTSAGQ EQTEDPHAWL DIENGIKYVE AKNYTEKLSK LHEEAKAKFA DIPDDKKLLV TSEGAFKYFS PEQMTTIIDT IKKSKAPVLF VETSVDKRSM E-RVSX EVKRP YSMNWNLTK IHOGLMSK

ETGGNGWFNI(

NIRDVLVEXKD

KAYDLNAAYI

TYDTLFTDSL

LMKTAKKVEN

PKNKDFYTEN

WEINTESQOT

AKEGTEGDTY

EF009-1 (SEQ ID NO:29) 9 9* 9 9* 9 9 9 9 9*9 9 9 9 *9*9 9*9*

TGACAAATGA

GCAGGTTGTG

GTAGGAACAA

GATTIACAAT

GAAATTGA NT

GCAACGAAAT

AAATTGAAAG

ACGAATGGCG

GCGAAACAGC

ACTGAATTAG

AATAGCGGCA

CCTGTAAACG

GAGAATAAAC

ATTGCAGAAA

AAAAATT-TAG

CATCGGGGTC

AAAATGATGA

TGGTAGAATT

TAAATGCCTT

TAGTATCAAT

ATATCACGAA

GGCGGGCGTT

AACTACCGAC

ATGCTACGCA

TGGCTGTCGA

AAAA.AGCGAA

TTTATCAAAA

.CATCAAAAGG

TAAATTAATT

TGTGAAGGAT

ATGG.GAATCG

TACAGACTAT

TCAGCATCAA

TGGCAATACA

AXITAAAGAC

AATITTTATTA

TGTCAGTGAT

AACAGCGCGC

TGCTGGGTAT

ACCTTATGTG

AGTGTAGAA

CGCCAATGTT

GGACTTATTG

ACTAAGACAG

GTCAAAGACC

ACGCAACCAA

ATCT=rTAG

GTCAATGCAC

GGCGGAGAAA

CAAACTGCAG

ATACTGAAA.

rGc'TrrAcAAG

ACACCAGATA

AACATrCTCG

GAATATCAAC

CCAGCCTGGG

GGGTATTAGC

AAACCGTTAA

GTTTAAAA

ACGCAGCATT

ACAATTACAA

CATI'GGGAAT

TTGCTATTCC

GACTGATAAA

ATAA-ACGCCA

ATGTCGATGC

AAGATGCTAT

TGGCCCGAGA

A.AGAAGAAAC

AAACArIG

TTTTACGATT

ACTAGGGGTT

GAAAAATATT

AGCAGAAAAA

TAAAGAGCAT

TTACGCTAAT

TAATGACCCA

AGTAGATCCT

ATTGAAAATA

TTCAGTGATT

TFICTTAGA-A

AGAAGATCAA

GAAAAAGGTC

TAAAAAATAA

EF009-2 (SEQ ID MKKFSKL IG L IGVLAFTIA GCASGSVKDT KTETTKLGVV GTKNDEWdESV KDRLKKKNID LQLVEFTDYT QPNAALAEXE IDLNAFQI4QI FLDNYNKENG TKLVSIGNIV NAPLGIYANK LKDITKIKDG GEIAIPNDPT NGGRALILLQ TAGLIKVDPA KQQLPEVSDI TENKRQLKIT E-LDATQTARA LQDVDASVIN SGMAVDAGYT PDKDAIFLEP VNEKAKPYVN IVVAREEDQE NXLYQKVVEE YQQEETKXVI AETSKGANVP AWETFGKK EF009-3 (SEQ ID NO:31) TTGTG CATCGGGGTC TfGTGAAGGAT ACTAAGACAG AAACCGTTA.A ACTAGGGGT

GTAGGAACAA

GAT'ITACAAT

GAAATTGATT

GGAACGAAAT

AAATTGAAAG

ACGAATGGCG

GCGAAACAGC

ACTGAATTAG

AATAGCGGCA

CCTGTAAACG

AAAATGATGA

TGGTAGAATT

TAAATGCCTT

TAGTATCAAT

ATATCACGAA

GGCGG.GCGTT

AACTACCGAC

ATGCTACGCA

TGGCTGTCGA

AAAAAGCGAA

ATGGGAATCG

TACAGACTAT

TCAGCATCAA

TGGCAATACA

AATTAAAGAC

AATFI'TATTA

TGTCAGTGAT

AACAGCGCGC

TGCTGGGTAT

ACCTTATGTG

GTCAAAGACC

ACGCAACCAA

ATCTFI'TTAG

GTCAATGCAC

GGCGGAGAAA

CAAACTGCAG

ATTACTGAAA

GCTTTACAAG

ACACCAGATA

AACATTGTCG

GTTTGAAAAA

ACGCAGCATT

ACAATTACAA

CATTGGGAAT

TTGCTAMTCC

GACTGATAAA

ATAAACGCCA

ATGTCGATGC

AACA GCTAT

TGGCCCGAGA

GAAAAATATT

AGCAGAAAAA

TAAAGAGCAT

TTAcG~cTAAT

TAATYCACCCA

AGTAGATCCT

AI'GAAAATA

TTcAGTGATT

TTTCTTAGALA

AGAAGATCA

87 TABLE 1. Nucleotidle and Amino Acid Seqeuences of E. faecalis Genes.

GAGAATAAAC TTTATCAAAA AGTTGTAGAA CAATATCAAC A.AGAACAAAC GAAAAAGGTC ATTGCAGAAA CATCAAAAGG CGCCAATGTT CCAGCCTGGG AAACATTTGG TAAAAA EF009-4 (SEQ ID NO:32) CASGSVKDT KTETVKLGVV GTKNDEWESV KORLI(KKNID

LQLVEFTDYT

LKDITKIKDG

ELDATQTARA

NKLYQKVVEE

QPNAALAEKE

GEIAIPNDPT

LQDVDASVIN

YQQEETKI<Vl

IDLNAFQHQI

NGGRALI LLQ SCfrAVDAGYT

AETSKGANVP

FLDNYNKEHG

TAGLIKVDPAk PDKDAI FLETP

AWETFGKK

TKLVS IGNTV NAPLGIYANK KQQLPTIVSDI TENKRQLjKIT VN'EKAKPYVN IVVAREEDQE 00*0 00..

0 0 0 0 EFOIO-1 (SEQ ID NO:33) TGAAAGAATA AAATTGTACA CGTAGCTGGC GCACAGCT T GCAGCGTCAA AAGGTGATGA CCAGATAATT ATGACCAATT GCAAAATTAA AAATGGAATT GTTGCTTCTG GTGAAAGCTA AAACGCGCCT ATGCTCATTT CAATTGCCAG ATAACTATAT ATTTTAGGTA ACTCTTACGG TACAATTAG ATATTAGTAA GAATTCCNTA AAAANGANCC ACAGGTAATT ATGACTTCCC ACTGGCTCAC CAAAAATTAT GTCTTGCATC AATGGTATAA CCATATGACT TAALATACCAA TATGGTGATA ,CA.ATCTTAAC GAACCATTAA AAACAACAC AAAAACAAAG AAAAATCTGT AACGGACTTG TTTATGGTGA AAATrCGTTGA AAGATTACAC TTAATCAT= GGCCAGAAGA ATCGAAGAAG CAAAAGATC ACTGAAATCA CTAACGT1'QC ACTGTTGATC CAGAACAAAC

'GAGGAAATA

kGC'TGTAGGA rAGTACACCA k.ATCGATAAT rGTTGCTMG rGATATTTCA kACTGATTTA

TAAAGGAAAT

TCAACAAGTT

AGTCGATGGT

AAATATTGCT

TATTGGTAAC

TAACCAATAT

AGATGGCTI'G

TACTTGGTTT

ACAAGCTGCT

TCAAGCGCAA

TGAATTGTTA

AGAAGGCAAA

.ACCAACAACT

ATCTGTCACT

ACCAATTCTI'

TACAGTTATG

ACTTCCAAAA

GCAAACACAA

AGGAATGAAA

CTAAGCGCGT

ACGTTATTAA

GCGAATAAAA

GCGATTGCG

TTAGCACAAA

GCACCTAAAT

ACCATTAATC

TTAACTTTTA

AGTTATGAAA

GCTTI'TGCTA

CAATATCCAT

GCCGACAAAG

AITCCAACAG

ATGCGTCAAG

GGCAAACCAC

ATGGCTAACT

GGFFI'ATTAA

CAATATGAAA

CATTTGAGTG

GAAGAAATGG

GG TIrTACTT

AACCGTTACG

TTAATGGATC

TTAGACGAAT

AAATGGCAAA

GCGGTAAATC

TGTATCGTGT

TTATCGAGAA

ACCAAAAAAT

ATTAT'GCAAC

ATGCCAAAGA

GAAAACTGTA

ATAA.AGAATA

GTGCAACGGA

TCGGCCAAAC

TTCAGTAAA

ACATGATITAA

ATGCTCCTAC

A-AACACAAGG

TTGTTI'CTCG

ATGTTGTI'GC

ACAGCAATCC

AAGTTGGCGA

CTTGGAACAC

TTAA.AGAACG

T'TGTAAATGA

CAGCAAGCTT

ACCTAAAAAC

ATATCCAATC

AAGGATTAGC

TTCAAAAGAT

TGGGGACAAA

AAAAATTGGG

CTCAACAATC

GAATGCACAA

AGCCTATGAT

TGCGTTCCCA

TGTCGATAAA

AGI'CTAAAA

ATTCTTTGCA

AACAACTGAT

TA.ACTTAAA-A

AAGTACAACA

ACCTATGGAT

TCCACTAACA

AAACACGTCT

AGAATTGTTA

TGATCGTGTG

AGGAAACAAC

TGATAAGAC

TAAAGTGAAA

AAATACAGGA

AGCTGGCTGG

TCAAAAATAA

GATAAAGTTC AAAAAGAAAT EF010-2 (SEQ ID NO:34) MAKRISR SWRTALAVGL SACGKSSKDA ASKGDDSTPT LLMrYRVGDKP

ONYDQLIDNA

GAYADLTDLA

NLDI SKVDGS GSPKI INQYA

GDTILTQAAG

GLVYGEEGKQ

EEAKDSP ILG

KVQKEMQTQL

NIIIEKKIGA

PKYAI(EAYDQ

YESATEVLKE

DKDMINNLKV

KPLVSRPLTE

YEKVGDIJRVK

FTFVNDKVKT

DEYIQSQK

KLKMEFVGWG

LPDNYIKGNT

FXKXXPNIAA

LHQWYKDGLI

PLKTTrAQAQM LLf(DYTPTTH

EITUVATVMN

DWDQKM4STIV

INGKLYAFPI

FAIGQTFFAT

PTDAATSflrP

ANYVVANTSK

LSAWNTGNNL

RYAASLNTGT

ASGESYDISL

LGNSYGQQVL

GNYDFPIGNQ

YDLNTNTJiFM

NKEKSVELLG

I IWPEESVTE

VDPEETLPKL

AQNYATNAQK

TFNKEYVDKY

YPFAVKTTDT

RQETQGPMDy

LLNSNPELLN

EMVKERDKS I

MDDLKTAGWD

EF010-3 (SEQ ID 88 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. foecalis Genes.

CT GCGGTAAATC TTCAAA.AGAT

GCAGCGTCAA

CCAGATAATT

GCAAAATTAA

GTTGCTTCTO

AAAGCGCCCT

CA.ATTGCCAG

ATTTAGGTA

TACAATTTAG

GA.ATTCCNTA

ACAGGTAATT

ACTGGCTCAC

CTCTTGCATC

CCATATGACT

TATGGTGATA

GAACCATTAA

AAAAACAAAG

AACGGACTTG

AAATTGTTGA

TTAATCATT

ATCGAAGAAG

ACTGAAATCA

ACTGTTG'ATC

GATAAAGTTC

AAGGTGATGA

ATGACCAATT

AAATGGAATT

GTGAAAGCTA

ATGCTGATTT

ATAACTATAT

ACTCTTACGG

ATATTAGTAA

AAAANGANCC

ATGACTTCCC

CAA.AAAWIAT

AATGGTATAA

TAAATACCAA

CAATCTTAAC

AAACAACAGC

AAAAATCTGT

TTTATGGTGA

AAGATTACAC

GCCAGAAGA

CAAAAGATTC

CTAACGTTGC

CAGAAGAAAC

TAGTACACCA

AATCGATAAT

TGTrGGTTGG

TGATATTCA

AACTGATTA

TAAAGGAAAT

TCAACAAGTT

ACTCGATGGT

AAATATTCCT

TATTGGTAAC

TAACCAATAT

AGATGGCTTG

TACTTGTTI

ACAAGCTGCT

TCALAGCGCAA

TCAATTGTTA

AGAAGCCAAA

ACCAACAACT

ATCTCTCACT

ACCAATTCTT

TACAGTTATG

ACTTCCAAAA

ACGTTATTAA

GCGAATAAAA

GGCGATTGGG

ETAGCACAAA

GCACCTAAAT

ACGA=DATG

TTAACTTTTA

AG ITATOAAA

GCTTTTCTA

CAATATCCAT

GCCGACAAAG

ATTCCAACAG

ATGCGTCAAG

GGCAAACCAC

ATGGCTAACT

GGTTTATTAA

CAATATGAAA

CATTTGAGTG

GAAGAAATGG

GGTTTTACTT'

AACCGTTACG

TTAATGGATG

TTAGACGAAT

TGTATCGTGT

TIrATCGAGAA

ACCAAAAAAT

AITATGCAAC

ATGCCAAAGA

CAA.ACTGTA

ATAAAGAATA

GTGCAACGGA

TCGGCCAAAC

TTGCAGTAAA

ACATGATTAA

ATGCTtCTAC

AAACACAAGG

TTGTTTCTCG

ATGTTGTTGC

ACAGCAATCC

AAGTTGGCGA

CTTGGAACAC

TAALAGAACG

ITGTAAATGA

CAGCAAGCT

ACCTAAAAAC

ATATCCAATC

TGGGGACAAA

AAAAATTGGG

GTCAACAATC

GAATrCCACAA

AGCCTATGAT

TGCGTTCCCA

TGTCGATAAA

AGTTCTAAAA

ATTCTTTCCA

AACAACTGAT

TAACTTAAAA

AAGTACAACA

ACCTATGGAT

TCCACTA-ACA

AAACACGTCT

AGAATTGTI'A

TGATCGTGTG

AGGAAACAAC

TGATAAGAC

TAPIAGTGAAA

AAATACAGGA

AGCTGCCTCG

TCAAAA

a a a a a a a a.

AAAAAGAAAT GCAAACACAA EF010-4 (SEQ ID NO:36) CGKSSKDA ASKGDDSTPT LLMYRVGDKP

DNYDQLIDNA

GAYADLTDLA

NLDI SKVDGS GSPKI INQYA GDTI LTQAAG

GLVYGEEGKQ

EEAKOSPILG

NKIIEKKIGA

PKYAKEAYDQ

YESATEVLKE

DKOMINN~LKV

KPLVSRPLTE

YEXVGDDRVK

FTFVNDKVKT

KLKMEFVGWC

LPDNYIKGNT

FXKXXPN IAA

LHQWYKDGLI

PLKTTAQAQM

LLKDYTPTTH

EITNVATVMN

DWDQKMSTIV

INGKLYAFP I

FAIGQTFFAT

PTDAATSTTP

ANYVVANTSK

LSAWNTGNNL

RYAASLNTGT

ASGESYDISL

LGNSYGQQVL

GNYDFPIGNQ

YDLNTNTFM

NKEXSVELLG

I IWPEESVTE

VDPEETLPKL

AQNYATNAQI(

TFNKEYVDKY

YPFAVKTTDT

RQETQGPMDY

LLNSNPELLN

EMVKELRDKS I

MODLKTAGWD

KVQKEMQTQL DEYIQSQK EF011-1 (SEQ ID NO:37) TAACGT'I=rI

ATGGGACTGT

ACAGAAACAA

CCCAAAAATC

CTAGGT-GTCG

AAATACCAAA

CAACTAAAAC

AAAGCCATTG

AAACAAAATA

ATAACTGGCT

AATGCGCT'G

TTCGGTTTAA

CCAGGAAAAG

TAATGTTAAG

CAGCTAAAAC

CTAAGAAAGT

GTGACCGCGT

AAGTTrGAATC

CAGACTTAAT

CGCCAACCAT

TCGAAACGTT

TAGAAAAACA

TTCTGTTAGT

TTCATGATAC

AATCAAAAAG

TGCTTGTCAA

GGAAGTCACA

CGTIG ITTT1

GGTAGGTGCG

AGCAGGCGGC

TATrATTTCT

ITACTTAGCT

AGGCACTATT

AATrGCTGAC

TAACGAAGGA

ATTTGGCTTC

A;ATTFITAG

ACAAATAAA.A

GTCAAAGACA

GATAATGG Tr

CCAACTAAAA

ATTAAAGAAC

GGTCGTCAAC

GTAGATCCCA

TTTrGATAAAG

GTGAAAAAAC

CAACTTCCG

AAAGCAGCAG

CAATGATGGC

AAACAGCAGA

CCAATIGGTCA

CCTTGGATAC

ATATCCCTGC

CAGATITAGA

AAGAT1'ATCA

AAAATCCTTG

AAGAGGTAC

AAGCAGAAGC

CTTACGGAAA

ACGATAAGAT

AGTTI'CAATG

TTCTGCAACA

ATTAACCG1TT

AATGGATGCA

GTATTTGAAA

AAAAATCAAT

AGAACAATI'A

GGCATCAACG

TAAAGAAAAA

TAGCGCGAAT

ACCCTCTCGT

TGAAGCTTCC

89 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Gencs.

ACTCATCCGC

GTGGTAGATC

GAATTGATTC

GTTTGGTATC

AAAGGATTAG

AAAGTGTTTC TTACGAATAT G=TAGAAA AAAATCCTGG GATTCTCTTT GCACCAAAGC AATTGGTCGC CACGATTCA-A AAGATAACGT CGCTGCAAAC AAAAAACCGA TGCTGGTAAA AATGATAAAG TCATrATGCT TCAACCAGAT TAAGCGGTGG TGGATTAGAA TCAM'GCATT TGATGATAGA AGATGTTAAA

AGTAA

EF011-2 (SEQ ID NO:38) bMflKK FLAMMAVSMN GLLMLSACQT NKKTADSATT ETTAKTEVTV KDTNGQLTVP KNPKKWVVFD NGSLDTNDAL GVGDRVVGAP TKNIPAYLKK YQKVESAGGI KEPDLEKTNQ LKPDLIIISG RQQDYQEQLK AIAPTIYLAV DAKNPWASTK QNIETLGTIF OKEEVAXEKI TGLEKEIADV KKQAEASANN ALWVLVNEGQ LSAYGKGSRF GLIHDTFGF< AADDKIEAST HGQSVSYEYV LEKNPGILFV VDRTKAIGGD DSKDNVAANE T.IQKTDAGKN DKVIMLQPDV WYLSGGGLES MHLMIEDVKK GLE EF011-3 (SEQ ID NO:39) TTGTCAA ACAAATAAA.A AAACAGCAGA TTCTCCAACA

ACAGAAACAA

CCCAAAAATC

CTAGGTGTCG

AAATACCAAA

CAACTAAAAC

AAGCCATTG

AAACAAAATA

ATAACTGGCT

AATGCGCTTG

TTCGGTTTAA

ACTCATGGGC

GTGGTAGATC

GAATTGATTC

GTTGGTATC

CAGCTAAAAC

CTAAGAAAGT

GTGACCGCGT

AAGTCAATC

CAGACTTAAT

CGCCAACCAT

TCGAAACCTT

TAGAAAAAGA

TTGTGTTAGT

TTCATCATAC

AAAGTGTTTC

GCACCAAAGC

AAAAAACCGA

TAAGCGGTGG

G;GAAGTCACA

CGTTGTTTI

GGTAGGTGCG

AGCAGGCGGC

TATTATTTCT

TTACITAGCT

AGGCACTATT

AATTGCTGAC

TA-ACGAAGGA

ATTTGGCTTC

TTACGAATAT

AAT1TGGTGGC

TGCTGGTAAA

TGGATTAGAA

GTCAAAGACA

GATAATGGTT

CCAACTAAAA

ATTAAAGAAC

GGTCGTCAAC

GTAGATGCCA

TTGATAAAG

GTGAAAAAAC

CAACTTTCCG

AAAGCAGCAG

GTT'ITAGAAA

GACGATTCAA

AATGATAA.AG

TCAATGCATT

CCAATCGTCA

CCTTGGATAC

ATATCCCTGC

CAGATTTAGA

AAGATTATCA

AAAATCCTTG

AAGAGGTAC

,ACCAGAAGC

CTTACGGAAA

ACGATAAGAT

AAAATCCTCG

AAGATAACGT

TCATTATC.CT

TG-ATGATAGA

ATTAACCGTT

AATGGATGCA

GTATTTGAAA

AAAAATCAAT

AGAACAATTA

GGCATCAACG

TAAAGAAAAA

TAGCGCGAAT

AGGCTCTCGT

TGAAGCTTCC

GATTCTCTTT

CGCTGCAAAC

TCAACCAGAT

AGATGTTAAA

AAAGGATTAG AG EF011-4 (SEQ ID CQT NKKTADSATr EIAKTEVTVJ KDTNGQLTVP KNPKKVVVFD NGSLDTM4DAL GVGDRVVGAP TKNIPAYLKK LKPDLIIISG RQQDYQEQLK AIAPTIYLAV DAXNPWASTK TGLEKEIADV KKQAE-ASANN ALVVLVNEGQ LSAYCKGSRF HGQSVSYEYV LIEKNPG ILFV VDRTKAIGGD DSKDNVAANE WYLSGGGLES MHLMIEDVKK GLE

YQKVESAGGI

QOIETLGTIF

CLIH]DTFGFK

LIQKTDAGKN

KEPDLEKINQ

DKEEVAKEK I

AADDKIEAST

DKVIMLQDV

EF012-1 (SEQ ID NO:41)

TGAGGGGGCA

CTTTITAGCCG

GCAGCTGAAC

CAAACAACAG

TTTGATGATG

ACAACATGAA

CATGTGGCGG

AAAAAATCAG

ATAAAAATAC

ATAGTGCCAC

ATTGGGGAAA

AACCAAAGAA

TA ITAGTTCA C=rACAATC

GGTGCCAGCT

AAAGTAGTAG

GCGGCAGAGA

CCTGCACCAA

GCACAACATT

CTAGCTAAAG

GT=TTGC AACAGGGTITT AAGTAGA'ITC GGGAAATTTA.

TCTCAACATT GGATACAACA TATTTGA-AGG CCTTTATCGG A2'GTCA-AGAT TAGTGACGAT TABLE 1. Nucleotide and Amino Acid Seqeuences of E. [iaecalis Genes.

GGGCGCA.AGT

ACGGCCCAAG

AATGCCTATT

GTCGATGAAT

GCCCAACCTT

TGTCGAAG

CCATTTACGC

GAATACTATG

GATAATACTG

CALATATGTTC

TACTTCTTAG

GCGATTGGCC

AAACCCCTTA

TTCCGAGCTT

AC GAAAC C C

GATCAAGGAA

TTAGAAATTA

AATTATGAGT

AACTTATATO

CAATTC-GTAG

TACAAAGAAG

AGTGCCTCAA

ACCACI=AC

ATTTTGTTTA

TACTAGACAG

TAGGGATTTC

CCTTCTTAGC

CGCAAGGCAA

TAGCCAATTG

ATGCGGATCA

GGATTAACTT

AACAATATCA

ATI'CAACAA

AAGCAATTGA

ACGGATTGAT

ACAGTGGCGA

AAGCGGATGT

AACGAATGC

CCATTCATC

TGTCTCTTC

CAGGAGAATC

AAGAGGCACG

CGGAAGACAT

ATTATCTAAT

CTTGC-GGGAG

TTCTTGGAAA

TGTTAAAAAT

AGCCCCGAAT

ACTCGTTTCG

AGATTACGCC

G'OATGCGACT

AGTGAAACTG

ATATCAAGTG

AGATGATCCA

AAAAGAAGGA

TAAAGAAGCC

TCCAAGTAAA

ATATTTGAAA

CGGTAAAAAA

TGAATATGTT

GCAACCAAGT

AGGATGGATT

AAGTTACAAT

AACGATTAAT

CTTGTTGAAC

CAATCCTAAA

TGCCTATTTA

GGCGATTAAGT

AAACTGGTGA

AGTTTTGAAA

GACAAAGAAT

AT'GCTTGGT

TTGGATAGTG

TCAGATACTT

GAAGAAGTTG

AATGAACTAG

GGCTATGTCA

ACGCCATrAG

TTAACACAA.A

C ITATGCGA

AATGACGTCA

GTGAAA=T'

CAAAGTCAGT

AATAPATGTGA

GCCGGCAGTA

TACGGCAATT

GCCAATAATC

CAAGATGCTG

TTGAAAGGCA

ACAGAATGA

GGAGCAACGG

CACCAGCGAC

TACGCAACGG

TCATTGTTGA

TAGCGOCACA

AACA'ITTACT

GGACATTGAA

CGGTTAGCAC

ACTTAGTTCG

GTCATCCAGA

CGAATGTTCA

GTGTCTTAAA

ATCCAGAAAC

AAAAAGWTCA

CATTGCTGGC

TGCA.AGAAAA

ACCAATCGCG

GTGAATTIAGA

ATCATAATGC

CAGAGAkACA C CCAAG TAGCC

TTAGTTATCA

CGAGCCAATC

GATTGGACCC

TGAAAAGTCA

A'FrAAAACAG

AAATCAAAAA

TTATAGCGGG

AAAAAATCCA

AATCAAAGAA

CATTAACGGA

TGTGGCCAAC

TTTACGAAAA

CGATGGGTCA

GGATGAAGAT

AGCTGAATGG

GGCAGACACA

TCTGCCAGGT

ACGTGAAAAA

CTCTTACTTT

CAAATACGAC

G"LTTGCAGAA

GCTGTATCAA

CT'TGTATGGG

GATTAT=TCC ACrI'GCGCAA EF012-2 (SEQ ID NO:42) IIKLGKI< VVGLIATFL LAACGGTKEA AEKVDSGNLA AEQK I SISSP APT STLDTTQ

TTDKNTFTMA

AQDFVYSWKK

QPSFLAVVSI

Y-YDADQVKLE

FLDFNKKEGT

RAYSGEYLKN

EITISSQPSN

LVEEARTINA

YFHLRNAYLT

QHLFEGLYRP

LVTPATIGPN

AWLAPQNQKF

EVAVSTIKED

PLANVHLRKA

DVKKAQAEW'T

NVNQSRREKN

NNPEKQFAEY

DDDSATVPAL

AYLLDSVKNS

VEAQGKDYAL

NTGINLYQVN

IGQAIDKEAL

KAQADVGKKV

YELSLSGWIA

KEAEDILLNQ

AKDVK I SDDG

FEIRNGEXSV

DSEHLLYSGP

ELDLVRINGQ

TQSVLNDGSK

KLSLLAADTD

GSSELDSYFN

DAAQVPLYQS

RKYHFTLREG

DELG ISAPND

FTLANWDATS

YVQQYQDDPG

PLNGLIPSKL

QGKRIAEYVQ

LYAGES SYNY

ASNYLINPKL

IKWSNGEPIT

KEFIVELKQA

DTWTLKKNPE

YVSH PDVANY

YANPETDEDF

SQLQENLPGL

GNYHNAKYD)Q

KG ISYHLYGD EF012-3 (SEQ ID NO:43) ATGTGCCGG AACCAAAGAA GCGGCAGAGA AAGTAGATTC GGGAAATTTA

GCAGCTGAAC

CAAACAACAG

TI'TGATGATG

GGGCGCAAGT

ACGGCCCAAG

AATGCCTATT

GTCGATGAAT

GCCCAACCTI'

TTT~GTCGAAG

CCAITTACC

GAATACTATG

GATAATACTG

CAATATGTI'C

AAAAAATCAG

ATAAAAATAC

ATAGTGCCAC

ACCACTI'TAC

ATTTGTTTA

TACTAGACAG

TAGGGATTTC

CCTrCTTAGC

CGCAAGGCAA

TAGCCAATG

ATGCGGATCA

GGATTAACTT

AACAATATCA

TATTAGTTCA

CTTTACAATG

GGTGCCAGCT

CTTGCGGGAG

TTCTTGGAAA

TGTTAAAAAT

ACCCCGAAT

AGTCGITCG

AGATTACCCC

GGATGCGACT

AGTGAAACTG

ATATCAAGTG

AGATGATCCA

CCTGCACCAA

GCACAACATT

CTAGCTAAAG

GGGATTAAGT

AAACTGGTGA

AGTI'TTGAAA

GACAAAGAAT

ATTGC~rhGT FrGGATAGTG;

TCAGATACTT

GAAGAAGTG-

AATGAACTAC-

GGCTATGTCA

TCTCAACATT

TA'TMAAGG

ATGTCAAGAT

GGAGCAACGG

CACCAGCGAC

TACGCAACGG

TCM'I'GTTGA

TAGCGCCACA

AACATI'ACT

GGACATTGAA

CGGTTAGCAC

ACTTAGTTCG

GTCATCCAGA

GGATACAACA

CCTTTATCGG

TAGTGACGAT

CGAGCCAATC

GATTGGACCG

TGAAALAGTCA

ATTAAA.ACAG

AAATCAAAAA

TTATAGCGG

AAAAAATCCA

AATCAA.AGAA

CA'ITAACGGA

TGTGGCCAAC

TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

TACTTCI'AG

GCGATTGGCC

AAACCCCTTA

TTCCGAGC IT

ACGAAAGCCC

GATCAAGGAA

TTAGAAATI'A

AATTATGAGT

AACTTATATG

CAATTG-GTAG

TACAAAGAAG

ATTTCAACAA

AAGCAATTGA

ACGGATTGAT

ACAGTGGCGA

AAGCGGATGT

AACGAATTGC

CCATTTCATC

TGTCTCTTC

CAGGAGAATC

AAGAGGCACG

CGGAAGACAT

AAAAGAACGA

TAAAGAACCC

TCCA.AGTAAA

ATATTTGAAA

CGGTAAAAAA

TGAATATGTT

GCAACCAAGT

AGGATGGATT

AAGTTACAAT

AACGATTAAT

CTTGTTGAAC

CAATCCTAAA

TGCCTATTTA

ACGCCATTAG

TTAACACAAA

CTITATGCGA

AATGACGTCA

GTCAAACrT'

CAAAGTCACT

AATAATG~lA

GCCGGCAGTA

TACGGCALAIT

GCCAATAATC

CAAGATGCTC

TTGAAAGGCA

ACAGAA

CGAATGICA

G-TGTCTTAA-A

ATCCAGAAAC

AAAAAGCTCA

CAGCTGGC

TGCAAGAAAA

ACCAATCGCG

GTGA.ATTAGA

ATCATAATGC

CAGAGAAACA

CC CAAGTACC

TTAGTTATCA

=~ACGAAAA

CGATGGGTCA

GGATGAAGAT

AGCTGAATGG

GCCAGACACA

TCTGCCAGCT

ACGTGAAAAA

CTCTTACFT

CAAATACGAC

GTI'TGCAGAA

GCTGTATCAA

CTTGTATGGG

AGTGCCTCALA ATTATCTAAT GATTATTTCC ACTTrCGCAA EF012-4 (SEQ ID NO:44) CGGTKEA AEKVDSGNLA AEQKISISSP APISTLDTTQ

TTDKNTFTMA

AQDFXIYSWKK

QPSFLA'JVSI

YYDADQVKLE

FLDFNKKEGT

RAYSGEYLKN

EITISSQPSN

LVEZARTIN~A

YFHLRNAYLT

QHLFEGLYRF

LVTPATIGPN

AWLAPQNQKF

EVA VSTIKED

?LANVHLRKA

DVKKAQAEWT

NVNQSRREKN

NNPEKQFAEY

DDDSATVPAL

AYLLOSVKNS

VEAQGKDYAL

NTG INLYQVN

IGQAIDKEAL

KAQADVGKYV

YELSLSGWIA

KEAEDILLNQ

AKDVKISDDG

FEIRNGEKSV

DSEHiLLYSGP

ELOLVRINGQ

TQSVLNDGSK

KLSLLAADTD

GSSELDSYFN

DAAQVPLYQS

RKYHFTLREG

DELGISAPND

FTLANDATS

YVQQYQDDPG

PLNGLIPSKL

QGKR IAEYVQ

LYAGESSYNY

ASNYLINPKL

IKWSNGEP IT

KEFIVELKQA

DTWTLKNPE

YVSHPDVANY

YANPETDEDF

SQLQENLPGL

GNYHNAKYDQ

KGISYHLYGD

EF013-1 (SEQ ID

TAACGAAAAA

AGTCTAGCAG

GAAACAAAGA

TCTGTCACGG

CCTTCTGCAG

ATTACAATTA

CCTAATGCAA

TATGCGGATG

TTAATCGCA-A

GGACAAGAAG

TCTGGTAAAA

ATTTTAGCAA

GGCACAAAA.A

GATTTACCAA

TGAAAAAAAT

GATGTGGAAA

AAGCCACGCA

GTCAAAATTC

GA.ACTGATCA

CCAATG'rTGT

CCATTACAGC

CCAATGGCAA

CCGTTGATCA

CAGCGCTTC

CAGCACCGAA

GTTTI'ACTGC

ATCGCTTAGA

ATTAA

TGCTITGTTC

CAAAAAAACA

GAAATCCTCT

TAATGTGACA

AACGAATGAA

TTTCAATCCT

AACGGTAGTC

TTTTACAGTA

AGTATGTTAA

GCAAGCACAA

ACCCAACAAG

GGGGAAAATC

GTCCCTCA-AA

GAAAGAAATG

GGTGATGCTT

ATTAGTCCCA

CGTTCAGTGT

ATGATTCTAA

AAATGAAAAG

CGTCAGAAAA

ACCAAGCACC

AAATTAATGG

CTGCACAAGC

GAGCGGGAGC

AAAFI'GATAT

ATCCGAAACA

GTGCAGATGA

CAGCCAGTAA

TAGGGACACC

ATTGTCTTTA

GCCA-AAGCAA

TAGTCATTCG

TGCCACGCAG

TGATACAAAC

TACTACATTA

AGGTGTITT

GACTACTCAA

TCCAAGTTCA

AGCGACAATT

TGCGCGGGTG

T'ITAGT'C C C

TTACTTGTIT

ACGGAATAGT GCACCTGTCC TTTTAGCAAT ATTACCAT1'C TGCAACTATT TTAGTGTCAC GGATGCCCAA GGGAAITTCA TGTTACGFTA AATGGAGAAA EF013-2 (SEQ ID NQ:46) MKKIALFS MLTFSVLSLS LAGCGNKKTA STNDSKPKQE TKKATQKSSS QQEMKSSHSS VTGQNSNVTG ENPSENATQP SAGTDETNEV PQNQAPDTNI TITNWVFNPE RNEINGTTLP NATITATVVG DASAQAGVFY ADANGNFTVI SPRAGATTQL IATVDQRNSA PVQIDIPSSG QEAALSFSNI TIDPKQGTIS GKTAPNATIL VSRADDARVI LASFTADAQG NFTASNLVPG- TKNRLDVTLN GEIGTPYLFO LPN TABLE 1. Nucleotide and Amino Acid Seqeuences; of E. faecalis Genes.

EF013-3 (SEQ ID NO:47) ATGTGGAAA CAAAAAAACA GCAAGCACAA ATGATTCTAA GCCAAAGCAA.

GAAACAAAGA

TCTGTCACGG

CCTrCTGCAG

ATTACAATTA

CCTAATrCAA

TATGCGGATG

TTAATCGCAA

GGACAAGAAG

TCTGGTAAAA

AT'ITIAGCAA

GGCACAAAAA

AAGCCACGCA

GTCAAAATTC

GAACTGATGA

CCAATGT'rGT

CCATTACAGC

CCA.ATGGCAA

CCGTTGATCA

CAGCGCT~rC

CAGCACCGAA

GTTTTACTGC

ATCGCTTAGA

GAAATCCTCT

TAATGTGACA

AACGAATGAA

TTTCXATCCT

AACGGTAGTC

TTTTACAGTA

ACGGAATAGT

TTTTAGCAAT

TGCAACTATT

GGATGCCCAA

TGTTACGTA

AGCCA.ACAAG

GGGGAA.A-ATC

GTCCCTCAAA

GAAAGAAATG

GGTGATGCCI

ATTAGTCCCA

GCACCTGT'CC

ATTACGATIG

TTAGTGTCAC

GGGAATTTCA

AATGGAGAAA

AAATGAAAAG

CGTCAGAAA-A

ACCAAGCACC

AAATTAATGG

CTGC-ACAAC

GAGCGGGAGC

AAATTGATAT

ATCCGAAACA

GTGC AGATGA CAGC CAGTAA TAGGdACACC

TAGTCAI'TCG

TGCCACGCAG

TGATACAAAC

TACTACATTA

AGGTGTTITT

GACTACTCAA

TCCAAGTTCA

AGGGACAATT

TGCGCGGGTG

?I'TAGTTCCC

TTACTTGTTT

GA'I"I'ACCAA AT EF013-4 (SEQ ID NO:48) CGNKKTA STNDSKPKQE TKKATQKSSS QQENXSSHSS VTGQNSNVTC ENPSENATQP SACTDETNEV PQNQAPDTNI TITNVVFNPE RNEINGTTLP NATITATVVG DASAQAGVFY ADANGNFTVI SPRAGATTQL IATVDQRNSA PVQIDIPSSG QEAALSFSNI TIDPKQGTIS GKTAPNATIL VSRADDARVI LASFTADAQG NFTASNLVPG TKNRLDVTWN GEIGTPYLFD LPN EF014-1 (SEQ ID NO:49)

TGATG--TGGA

AGCTTAATTA

GTAGCGGGTA

TATAATGAAG

ATI'GAACGGA

AAGACGAAGA

AAAATTACC

TCTGTTAACA

TTTI'ATCTT

GCGTTTGATG

CATI'TAGATG

ATGCGTGGAT

CAATCAATCA

GTGGATTCCA

AAACAACAGC

CTATACCCAG

AAGCCAGATG

CAAAGTGATT

GGCAATACGT

ACGGAAAATG

GACTTTTITAA

GTTTAGTCAT

GCTATTTAAA

CGCAAAATAA

AATTAGGCAC

AAATAACCTA

AAGGGATGCA

CAGTI'GAGAA

TTATTAAGTT

GTGTCACCAA

GTACGAAAGC

TCCGACAACA

TCAAA-ATAAT

ATGAATTGAC

T ITCTTTTGA

ATAGTATTGA

AACGAGATCA

ATACCGTTCA

ATATTGGTGT

GCTI'TATAAA

GAGAGAGGAA

CATTTTGGTT

GAAXACAATT

AGATAGTCAA

AACTAGGACT

TTTAAGT'TTG

GCGAATG-AA

ATTATTGAAT

AATTGATGCG

AGAC'-GGCCA

TTTATCTTAT

AGAAATTATT

GGACATTATT

TCATTTAGTC

CTGGCGCACT

AAATGTCCCT

AGACGGCTAT

AGATGAAGCA

TCCTGGTAAT

ATAA

GTACAGCCAA

TTTGTCACAG

GATAAAGGCT

TCGTTTTTGA

GATGCTATGA

CCACGGGATA

CCCGCCTATA

ATTCCAATCA

GTTGGCGGCA

GGATCCATTC

GCCCGTGAAA

CAAGCAGTTG

GATTCGTTAA

AAAGAAGGTT

r=AGTAATG CATCAATTAc

GTCTTCCATA

GCTGAGGAAA

ACACAGACCG

TGAC-TAGGAA

TCGGCTCAGC

ATGTTCCCAT

TTATGGGGCT

TGGTGA'ITAC

GTTTTGTTCA

CCTACGATGG

ATCATI'ACGT

TAGATGTCAA

ATTTTATGC

GACATAGCGA

AAGACAAGTT

ATGGAAACAT

TGACTTCGAC

AAGGGCGCAG

GTGTGTCTTT

CGAACGGTGA

ACGAAATGAC

GCCCGTTGCC

GCGAAAAATC

ATACTTTGCT

AAAAAATGAT

AGACAATACA

CGTGAATAAC

AATTGATGCG

ACCAACACCT

TGTGTTTAAC

TGTCAAGCAG

ACGGAAACAG

TAACGATATT

GAAATCTGGT

TCAAACTGAT

CAATTATGAT

TATGGTTGAA

AAATTTAGAA

ATTTTTATAT

TTCCATCA.AC

ATCAGTTAAA

EF014-2 (SEQ ID MSRKRKIS LISLVIILVF VTVGSAYFAV ACGSYLKKTID KGYVPIKNDY NE-AQNXDSQS FLIMGLDNTI ERKLG~TTRTD ANMVITVNNX TKKITYLSLP NYQGMQRIEA AYTYDCPTAS vNTVEKLLNI PINHYVVFNF LSFIKLIDAV FDGVTKDGPG SIHFDAGKQH IJDGTKALSYA RERHSDNDIM RGFRQQEIIQ S IMKIMDI ID SLNGNIQTDV DSNELTHLVK EGLTWTNYDK QQLSFDWRTF RDSFVQ IDAK

GGIDVNVKQA

AVEDKLKSGQ

SNEGRSMVEL

TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

YPDSIENVRH QLRVSLNLEK PDERDQDGYV FHTNGEFUYQ SOYTVQDTEAA EENEI'TSING NTYIGVPGNT QTGPLPSVKT

ENGFIK

EF014-3 (SEQ ID NO:51)

TGCT

GTAGCGGGTA

TATAATGAAG

ATTGAACGGA

AAGACGAAGA

AAAAkATTACC

TCTCTTAACA

TTTTTATCT

GCGTTTGATG

CATTTAGATG

ATGCGTGGAT

CAATCAATCA

GTGGATTCCA

AAACAACAGC

CTATACCCAG

AAGCCAGATG

CAAAGTGATT

GGCAATACGT

GCTATTTAAA

CGCAAAATAA

AA'ITAGCAC

AAATAACCTA

AAGGGATGCA

CAGTTGAGAA

TTATTAAGTT

GTGTCACCAA

GTACGAAAG-C

TCCGACAACA

TGAAAATAAT

ATGAATTGAC

TTTCTTTTGA

ATAGTATTGA

AACGAGATCA

ATACCG ITCA

ATATTGGTGT

GAAAACAATI' GATAAAGGCT AGATAGTCAA TCCTT'rTTA AACTAGGACT GATG TATGA TTTAAGTTTG CCACGGGATA GCGAATTGAA GCCGCCTATA ATTATTGAAT

ATTCCGAATCA

AATTGATGCG GTTGGCGGCA AGACGOCCA

GGATCCATTC

TTTATCTTAT GCCCGTGAA.A AGAAATTATT CAAGCAGTTG GGACAFTATT CATTCGTTAA TCATTTAGTC AAAGAAGGTT CTGGCGCACT

TTTAGTAATG

AAATGTCCGT CATCAATrAC AGACGGCTAT GTCTTCCATA AGATGAAGCA G-CTGAGGAAA TCCTGGTAAT ACACAGACCG

ATGTTCCCAT

7 kTGGGGCT

TGGT'GATTAC

GTr'rrTTCA

CCTACGATGG

ATCATTACGT

TAGATGTC-AA

ATTT'r1'ATGC

GACATAGCGA

AAGACAAGTT

ATGGAA.ACAT

TGACTTGGAC

AAGGGCGCAG

GTGTGTCTITT

CGAACGGTGA

ACGAAATGAC

GCCCGTTGCC

AAAAAATGAT

AGACAATACA

CGTGAATAAC

AATTGATGCG

ACCAACAGCT

TGTGTTA-AC

TGTCAAGCAG

AGGGAAACAG

TAACGATAT

GAAATCTGGT

TCAAACTGAT

CAATTATGAT

TATGGTTGAA

AAATTTAGAA

ATTTTTATAT

TTC-CATCAAC

ATCAGTTAAA

a. a.

a a a a.

a ACGGAAAATG GCTTTATAAA A EF014-4 (SEQ ID NO:S2) AV AGSYLKKTID KGYVPIKNDY NEAQNKDSQS FLIMCLDNTI

ERKLGTTRTD

NYQGMQRIEA AYTYDGPTAS VNTVEKLLNI FDGVTKDGPG SIHFDAGKQH LDGT-KALSYA SIMKIMDIID SLNGNIQTDV DSNELTHLVK YPDSIENVRH QLRVSLNLEK PDERDQDGYV NTYIGVPGNT QTGPLPSVKT ENGFIK

A&DVITNNK

P INMYVVFNF

RERHSDNDIM

EGLTWTNYIDK

FHTNGEFLYQ

TKK ITYLSLP LSF IKLIDAV RGFRQQE 10I

QQLSFDWRTF

SDYTVQDEAA

REDSFVQ IDAK

GGIDXTNVKQA

AVEDKLKSGQ

SNEGRSMVEL

EENTEMTSING

EF015-1. (SEQ ID NO:53)

TAATTAAAAA

TTAATAGCTA

AAAAGTTTTG

ATAAATTTAG

AATACTATAG

ATTGCGGTGA

AGATTCATT

TGTGTAAAAA

TCTCT'lITTC

ACGACACTAA

ATGA'ITCAAA

AAGITAAAAA

AAACAGGATG

TAGAAATTGT

GGGTCTGATG

TCCATAT=I

ATATGCTGTG

AGAATTTATC

TGGGCACGTT

GATATCAGAA

TCAACA.ATAT

AAAAAAGGAG

A'FFTTL'TTC

GTCAAGATAG

AAAACATATT

CGTGTAAAAA

CCAGGGCNAA

TCTAAGGATT

ACATA.ATAGT

ACAATAATCC

ATGGGAAAGA

ATCCATCAAA

AAGATAATAG

CTAGTATCTC

ATTATAT'ITA

TATTATCTTT

ATI'TAACTCC

GATTrGAGCGT AGGGcAATAT

TCCAGATCAA

TATTcCTCAC

CTAA

EF015-2 (SEQ ID NO:54) MK KGDIIVIIFL IAISFSPYF1 FFHNNPFNSK SFDDTKYAVV KIDGKEIERI NLDDSKEFIK TYYPSKGQYN TIEVKNGHVR VKKONSPDQI AVKTC;WISEP

GXTSICIPHR

FILEIVOQYS KDYYIY EF015-3 (SEQ ID TABLE 1. Nucieotide and Amino Acid Seqeuences of E.f[aecalis Genes.

CAATAATCC ATTTAACTCC AAAAGTTTG ACGACACTAA ATAAATTTAG ATGATTCAAA AATACTATAG AAGTTAAAA-A ATTCCGGTGA AAACAGGATG AGATTCATTT TAGAAATTGT

ATATGCTGTG

AGAATTTATC

TGGGCACGTT

GATATCAGAA

TCAACAATAT

GTCAAGATAG

AAAACATATT

CGTGTAAAAA

CCAGGGCNAA

TCTAAGGATT

ATCGAAAGA

ATCCATCAAA

AAGATAATAG

CTAGTATCTG

ATTATATTTA

GATTGAGCGT

AGGGCAATAT

TCCAGATCAA

TATTCCTCAC

EFO1S-4 (SEQ ID NO:56) NNPFNSK SFDDT-KYAVV KIDGKEIERI NLDDSKEFIK TYYPSXGQYN TIEVKNGHVR FILEIVQQYS KDYYIY VKKDNSPDQI AV-KTGWISEP GXTSICIPHR EF016-1 (SEQ ID NO:57)

TGACCOTTGC

TTGCTGGTTA

GAAGATCAAT

TTTGTGCCCA

GCCAA.AGCGG

ATGAAAGAAA

ACGAGCGAGC

CTTGTTTCTT

GGGGTTCAAA

AAATTTGTTA

TTAAAATCTG

TCCCACGAAG

TTTGCTGTGG

GAAACGI'AC

GTTACAAATA

TCTGTTGTAG

GGACACGGAT

TGGG ITTTCA

FTTTAAACT

CAGAATTACA

GGGCCGAAAA

TAAAAGAAAA

ACGGCTCTTC

AAGACCAAAC

CTCGAATTGA

ATAA TI'AAA

GCGTCCGCAA

GAAAAGATG

ACACAPI.AAT

TTACTCCTA

TAACGAAGAA

AGATAAATCA

TTATGGCATT

AAATCAAACC

AGTTCAATTC

AAACATTGCA

TGGCTATGAT

ACCTA'TrA

CGGACTCCTA

AAACTATACT

ATCAGACAAT

CACCCTTAGT

AAACTAA

CCCCGTCCAA TAGAAAGGAG

TTTATGATGA

TTGCAGGTT

AAACGGATTA

GGCAAAATTG

TCCGITGACT

ATTGATCTTA

ACACAACCTT

ACAGCGAGCG

GGCTTCGAAA

TATGACGGCT

ATCGATCGCG

ATTTCTCATG

CAATTAGTCC

AAAATTTCTC

AAAAGAAATA

GTGGTAAAAG

TTATTGCTT

TCGGCITTGA

TCCAACCGAT

=TGGAACGG

ACATGACGAA

ACATGCALAGG

GTCAGCCTGA

TTAATGAAGC

TrrACCCCAA

TAGGC-TATGA

AAAAAATCAA

AAAAATGG;TT

TTCTTTAGCC

AAAAAGCA.AC

AGATGACTCC

TGTCGACTTA

TGAITTGTCT

CTACACTAAA

CGACCAAGTA

CAAAATTTTA

CGTTTTGAAA

TTTCTTAGAT

CTACTATCTT

CAATGAAGAT

TACTGCCTT

TGGAGAGGAC

EF016-2 (SEQ 10 NO:58) M1VflKKYSLAL

VPMGFQDKSG

SERAEKVQFT

FVKDQTPILY

AVG VRKSDNQ

LVICCSLLLF

KIVGFDVDLA

QPYMTNDQVL

DGFNEAFLDL

LVQKINTAFE

AGCGKRXSNE

KAVFKLYGIS

VSL

1

XEKNIAT

KSGRI DGLL I

TLRKDGTLSK

DQWTRINEEK

VDFQPIDWSM

ASDMQGKILG

ORVYANYYLS

ISQKWFGEDV

RIIIGLDDSF

KETELQNQTI DLIWNGYTKT VQNGSSGYDG FESQPDVLKK HEDNLKNYTI

SHVGYDNEDF

TNNTKIN

EF016-3 (SEQ ID NO:59)

AAGCAAC

GAAGATCAAT

TTGTGCCCA

GCCAAAGCGG

ATGAAAGAAA

ACGAGCGAGC

CTrGTTTCTT

GGGGTTCAAA

AAATrITGTTA

TTAAAATCTG

GGACACGGAT

TGGGTI'TTCA

IT'TIAAACT

CAGAATTACA

GGGCCGAAAA

TAAAAGAAAA

ACGGCTCTTC

AAGACCAAAC

GTCGAMTTGA

TAACGAAGAA

AGATAAATCA

?TATGGCATTr

AAATCAAACC

AGTTCAATTC

AAACATTGCA

TGGCTATGAT

ACCTAITTA

CGGACTCCTA

AAACGGATTA

GGCAAAA'FTG

TCCGTTGACT

ATTGATCTI'A

ACACAACCTT

ACAGCGAGCG

GGCTCGAAA

TATGACGGCT

ATCGATCGCG

TTATTGGCT

TCGGCTTTGA

TCCAACCGAT

TTTGGAACGG^

ACATGACGAA

ACATGCAAGG

GTCAGCCTGA

rTrA.ATGAAGC

=I~ACGCCAA

ACATGACTCC

TGTCGAC ITA

TG;ATTGGTCT

CTACACTAAA

ccGACCAAGTA

CAAAATTTTA

CGTTTGAAA

'FTTCTTAGAT

CTACTATC IT TABLE 1. Nucleofide and Amino Acid Sequences of E.jhecalis Genes.

TCCCACGAAG

T=TCTGTGG

GAAACGTTAC

GTTACAAATA

ATAATTTAAA AAACTATACT ATTTCTCATG GCGTCCGCAA ATCAGACAAT CAAT'TAGTCC: GAAAAGATGG CACC-CTTAGT AAAATTTCTC ACACAAAAAT AAAC TAGGCTATGA CAATGAAGAT AAAAAXTCAA TACTCCCTTT AAAAATGCTT TOGAGAGGAC EF01.6-4 (SEQ ID SNE DQWTRINEEK RIIIGLDDSF VPMGFQDKSG KIVGFDVDLA KAVFKLYGIS SERAEKVQFT QPYMTNDQVL VSLKEKNIAT FVKDQTPILY DGFNE-AFLDL KSGRIDGLLI AVGVRKSDNQ LVQKINTAPE TLRKDGTIJSK

VDFQPIDWSM

ASDMQGKILG

DRVYANYYLS

fSQKWFGEIDV KETELQNQTI DLIWNGYTKT VQNGSSGYDG FESQPDVLXK HEDNLKNYTI SHVGYDNEDF

TNNTKIN

EFC).7-1 (SEQ ID NO:61)

TGAGGTGTTI'

CTACTTCTCT

TTACGGGTCG

ACCGCAGCAG

GA.AGCGAAAC

ACTTTTACGA

TTGAATACT

TTTGA.AGGGT

GGGGTAACAG

TTCAACAAT

ACGGGCAAAA

GAAGGTTGGG

CAAGCGAATG

AAAAATCTTT

CAAGAACAAG

TTAA.ATACGC

TTGAATT'CTG

TTCGTGCCAA

GATTTAAATC

TTAGGAA~rG

ATCACTGACT

TCACCAGTTC

GTTrGGTGGCT

AAAAATITCCA

GCAAACGTAA

CAATTGGTTG

GTGGCCGATT

GTCTCTATCG

TTATGAAPLAG GGCAACAAAG CGGGCTGTOGG ALAGTGTTGGG GGATTGATTC GGAATTATCA ATG-TAATGAG CCAAGTAGGG CAGCATTGGQ AACTGAAAAA TTCGAAAAGA TGCAAAATGG CTTGGAAGCG CACAGTGGAC TAAA.AAATTA TCGTGCTATT CCATTGATGA CCATACCI'G TATTGGCGGT ACCAGCTI ACTATCCTAC ATCAGCTGAG ATGGCACGAA TAATACTTGG T'TTCGCTAGA TAAGGTGGAT TCGAAGGGAA AGAATTAGAT GCAATGCAGC TTTGAAAATT AAAAAGATCT ITTGGCAAAT AGCGTTTAGC TAAAALATGTT CAGGTTTCAC TAATCAAGAA CTAGTGAACC AGAAAAAGCG AAAAAGCGGA GCTAACGATT ATGTTCAAGG AGCTTTAGCA CTTTTAATAA TCGTTTAGAA GGACGCCAGT ATATGCTGAT ATAATITI'GG TAAATGGTCT CTTATGCAAA TAAATATGAA CGGAAGAAGC CCCCCTAGT CTG-TCCAAAA TTTAGTCTAT GCGACAAGTA A

'AAAGGCTGT

A.AAGAAACCA

ACGGCAGACG

.AGGGACTTT

GTACAGCCCT

AGTAACGGCG

CCAAAAACAG

G'ITGACGGTA

GAAGTAGAGC

TATCCTLrAA

TCAACACTTT

TCCTATGTGA

GTCCAAGTAG

GTIGTAAAAA

CGTGAAAITC

AAGAATGCAC

'ITAAATGATG

ACGCAAAAAG

AAAGAG'ITAT

TTAAGT1TCGG GATAATrrAG

AAAAGTCGCA

CCAATCGATT

AATAAGACCT

GAACG~TTGGA

CCTCTTTATC

GGTCCA'ITAG

:TTTGGCAGC

k.AAAGCAAGA

TGTCGTIGGC

TCTCCTrGA

CCAATGATGG

AGCCAATCAC

CTTCCCCGCA

GCAAATCTAA

TAAGCTATCC

ATGAAGCAT

ACAATGGCGC

AGAATAAAAA

TTAAAGAAGT

=ICTGGAGA

CTGGAACGTA

GTCGAGCAAT

GCTCAAAAAA

ATTTTGCAGA

GGCAA-ACGGC

ATACAGAAAA

AAAXT=AAC

GCGGAGAMT

TCTTAAACTT

TTGATCAGT

AAACATTACA

AATTrAACAGA

GTTCAGGCTA

AATCATGGTT

ACAACAGGTA

AALTGrGATAAT

CGAAAAAGGA

TTAAGCTAT

AGCAAATGAT

AGCGTATTAC

AGAAGAGTTA

TATGAGTTAT

TGTCGAAAAA

CTTCACATTA

TATTGGGAT

CAATACTGGG

AATTGTTGCA

'TTATATCCAA

AGCATTATCA

AGCACTTGGC

GGAATTAGGA

TAAAAAAGAA

TGCTAAAAAA

AGTCAATGTT

CGACATTGTG

ACTGCAATCA

GCTTCAAGAA

AAAAGCGGAT

AGCACGCTTA

TTACAAATCA

EF017-2 (SEQ ID NO:62) bflrRATKQ RLSLAAIMVL LLSGCGSVGK ETKKQE-QQVL RVGIDSELST ADVSLANDNT AADVMSQVGE GLFSFDEKGE AKPALATEKV QPSNDGLSYT FTIRKDAKWS NGEPITANDF EYSWKRTVDP KTASPQAYYF EGLKNYRAIV DGSKSI(EFELG VTAIDD{TrE IIELSYPMSYF QQLLAVPAFY PLNEAFVEKT GKNTYGTSAES TLYNGAFMIE: GWDGTIN 1WS YVKNKNYWDQ ANVSLDKVDXJ QVVKEVNTGK NLFE.GKELDV VKISGEIVAQ EQGNAALXIR EIPGTYYIQL NTQKDLLANK NARRAIAIJSL NSERLAKNVL NDGSXI(ALG-F VPTGFTNQET QKDFAEELGD TABLE 1. Nucleotide and Amino Acid Seqeuences; of E.faecalis Genes.

LNPSEPEKAX ELWQTAXKEL GIEKAELTIL SSDTENAKXI SEYVQGALAD NLENLTVNVS PVPFNNRLEK SRSGDFDIVV GGWTPVYADP IDPLNLLQSX NSNNFC: WSN KTFDQLLQEA NVTYANKYEE RWKTLQKADQ LVAEE-APLVP LYQLTEARLV AE)SVQNAJYG PLGSGYYKSV

SIGOK

ET017-3 (SEQ ID NO:63) CTGTGG AAGTGTTGGG AAAGAACCA AAAAGCAACA ACAACAGGTA TTACGGGTCG GGA'ITGATTC GGAATI'ATCA ACGGCAGACG TGTCGTTGGC

C

C

C C

ACCGCAGCAG

GAAGCGAAAC

ACTTTTACGA

TTTGAATACT

TTrGA.AGGGT

GGGGTAACAG

TI'TCAACAAT

ACGGGCAAAA

GAAGGTTGG

CAAGCGAATG

AAAAATCTTT

CAAGAACAAG

TTAAATACGC

TTGAATTCTG

TTCGTGCCA

GATTTAAATC

TTAGGAATTG

ATCAGTGAGT

TCACCAGTTC

GTTGGTGGCT

AAAAATTCCA

GCAAACGTAA

CAATTGGTTG

GTGGCCGATT

GTCTCTATCG

ATGTAATGAG

CAGCATTGGC

TTCGAA-AAGA

CTTGGAAGCG

TAAAAAATTA

CCATTCATGA

TATTGGCGGT

ACTATGGTAC

ATGGCACGAA

TTCGCTAGA

TCGAAGGGAA

GCAATGCAGC

AAAAAGATCT

AGCGTTTAGC

CAGGTTTCAC

CTAGTGAACC

AAAAAGCGGA

ATGTCAAGG

CTTTTAATAA

GGACGCCAGT

ATAATTTTGG

CTTATOCAA.A

CGGAAGAAGC

CTGTCCAAAA

GCGACAAG

CCAAGTAGGG

AACTGAAAAA

TGCAAAATGG

CACAGTGGAC

TCGTGCTATT

CCATACC'ITG

ACCAGCTITT

ATCAGCTGAG

TAATACTTGG

TAAGGTGGAT

AGAATTAGAT

TTGAAA.ATT

TTTGGCAAAT

TAAAAATGTT

TAATCAAGAA

AGAAAAAGCG

GCTAACGATT

AGCTTTAGCA

TCGTTI'AGAA

ATATGCTGAT

TAAATGGTCT

TAAATATCAA

CCCCCTAGTT

TTTAGTCTAT

GAGGGACTTT

GTACAGCCCT

A-GTAACGGCG

CCAAAAACAG

G ITGACGGTA

CAAGTAGAGC

TATCCTTTAA

TCAACACTTT

TCCTATCTCA

GTCCAAGTAG

GTTGTAAAAA

CGTGAA.ATTC

AAGAATGCAC

TTAAATGATG

ACGCAAAAAG

AAAGAGTTAT

TTAAGTTCGG

CATAATTTAG

AAAAGTCGCA

CCAATCGATT

AATAAGACCT

GAACGTTGGA

CCTCITIATC

GGTCCATTAG

TCTCCTTTGA

CCAATGATGG

ACCCAATCAC

CTTCCCCGCA

GCAAATCTAA

TAAGCTATCC

ATGAAGCATT

ACAATGGCGC

AGAATAAAAA

TTAAAGAACT

TTTCTGGAGA

CTGGAAC:GTA

GTCGAGCAAT

GCTCAAAAA

ATTTTGCAGA

GGCAAACCC

ATACAGAAAA

A-AAATTTAAC

GCGGAGATTT

TCIrA.AACTT

'I'IGATCAGTT

AAACATTACA

AATTAACAGA

GTTCAGGCTA

AATGGATAAT

CGAAAAAGGA

TTTAAGCTAT

AGCAAATGAT

AGCGTATTAC

AGAAGAGTTA

TATCAGTTAT

TGTCGAAAAA

CT1'CACATTA

'TATTGGGAT

CAATACTGGG

AATTGTTGCA

TTATATCCA.A

AGCATTATCA

AGCACTTGGC

GGAATTAGGA

TAAAAAAGAA

TGCTAAAAAA

AGTCAATGTT

CGACATTGTG

ACTGCAATCA

GCTTCAAGAA

AAA.AGCGC3AT

AGCACGCTTA

'rrACAAATCA EF017-4 (SEQ ID NO:64).

CGSVGK ETKKQEQQVL RVGIDSELST ADVSLAMDNT

AADVMSQVGE

EYSWKRTVDP

QQLL.AVPAFY

ANVSLDKVDV

NTQKDLLANK

LNPSEPEYAK

PVPFNNRLEK

NVTYANKYEE

SIGDK

GLFSFDEKGE

KTASPQAYYF

PLNEAFVEKT

QVVKEVNTGK

NARRAIALSL

ELWQTAKKEL

SRSGDFDIVV

RWKTLQKADQ

AKPALATEKV

EGLKNYRAIV

GKNY-GTSAE.S

NLFEGKELDV

NSERLAKNVL

GIEKAELTIL

GGWTPVYADP

LVAEE-APLVP

QPSNDGLSYT

DGSKSKEELG

TLYNGAFTLE

VKISGEIVAQ

NDGSKXALGF

SSDTENAKKI

IDFLNLLQSK

LYQLTEA-RLV

FTI RKDAI(WS VTAI DDHTLE

GWIDGTNNTWS

EQGNAALKIR

VPTGFTNQET

SEYVQGALAD

NSNNFGKWSN

ADSVQNLVYG

NGEP ITANDF

VELSYPMSYF

YVKNKNY'WDQ

EIPGTYYIQL

QKDFAEELGD

NLENLTVNVS

KTFDQLLQEA

PLGSGYYKSV

EF018-1 (SEQ ID TGTCATTACA ACGATACCAA TP1'TAATCAT TTATCCATTA CTACAAAAAC ACTTATCGG CGGTATGATG GCCGGTGCAG TAAAAGAATA AAGAAAGTAG GGA.ACAATAT GAAAAAAGTT TABLE 1. Nucleotide and Amino Acid Seqeucnccs of E. faecalis Genes.

TTAGCGCTT

AAGAAAGCTA

GCTTGGA.AGT

TGGACGGCC

GTGGATATTG

CATATGCCTG

TCTTCGGCCT

GTTAACCAAG

AATTACTCTA

GTTATI'CGTG

TTTGAAAA.AG

ACCACAGTCCG

CCTTAGAGG

TGGTTAAAAA

GATGATGGGG

CTGGAAC CA

TATATAGCCA

GGCAT'ITCAG

**ACTCAACTGT

GAAGCAGTTA

AAATTAGAAC

TACTTTAACA

ATGCAAGA AT

GATGCAGGAA

GTTATTITCAA

GCGGAAAACA

TATTCCTGC

GCTCAGATGT

TAGATAAGAA

TCCCFITGC

AATTTATTTC

ATATCGMAC

ATTCTTTAAA

ATACGTTTAA

ATACAAAAGC

AAGATGTCTA

TCATCCAACA

GCGATGGTGC

ATAAAAAZTGG

CATTAATCA

CAACGTTTGA

GTGGTCAAGG

TTGATGGACC

GTTGGTTAAG

TCACATATTT

CTTATCCCTA

AAACAGACAA

ACGACCGTGT

CCCCCAAAGG

CGCCGGAAGC

TGATTCGTGC

TAAAATCAAA

GAGACAAACT

AACGGCGGTC

CTCA-A'TAAG

GA.AAGAACCG

AAAAGACCTG

CGGCGATGAT

ATTAACTGAA

CGAT-TTAGCT

ATGGTATCC

GGATTATGAA

TAATGCATTA

GATTAAAGAA

AGGACCATTT

TAAATACTAT

TGTTTACCGA

TGAAAAkAGTG

TGGGAACTTC

AAGTAGCACT

TAATACATT

AATTGATGAA

CA.ATATCALA

TGATGCCTAC

CAATAAACTA

AAAATTAGTC

TCGTGCGAAT

GAAAGATGAT

TAAATGGGAT

TAAGTAA

GTTAGTTTAG CGGCCTGTAG CGGT-,GGGAA GATCGGTA 1G AATTAGATGA AAAGACGCCT ACCAAGATTA AATGGTATAT TAACTCAGAT ACCACTGCGC ACATTAAAAA AGACTTAAAT TCAAAkATTAA ATGCCATGAT TTICAAGTGGA AAAACTGGAC A-AGCAGCATT GAAAGCAGAT AAAAAATATG ACCCCTATTT AATGAA.AGTT TTAGAGCA 1G GA.AAAACATA TCCTIACCCT AGTGGAAATA TCCCAGTAAA TGATAAT TIT GGCAAGCCAG ACGTITCAAC ACCAGAAAAT AAATATCCTC ACATGACCCC AATGGGCTTC TTAGACAAAT TACAAGACTT CTTAGGTCTT GATCGAAA'IT TAGATAAAGA ATATTTAGAA GCAGGCAATA 'ITAGtCATGA TAGCTTCACA AAACAAGGAA ATTATGCAAC CATGCTCGTT ACAGAATTTA TGAAAAAATC TGGCACACGT TCTGGCCGAA AACCAACATr AAATCAAACC ACGAAAGATG CGAAACATCC AGCAAAACTC CCGGGACAAkA T'ITTAACAAA ATATGGCGTT GGAAAAATTCG ATTATrTACC AGAAGTGAA.A AACAAAAAAT ATGGCArAG TCGTITCCTA AAAGTACCAA TGGAAAGTGC TTTAACGCALA CCACATTTCG TAATTGAAA-A TATTAATCCA GAACCAITT AAACCAAACT AAATACAACC AAAGCCTTTG ACAAATCTTT AGAAGACTAC GCAATTGAAA AAATAAAATC TGAGAAAATG *99*9* 9 9*99 9 99*9 9- 9 9. 9 99 EF018-2 (SEQ ID NO:66) MKKV LGGLLVATAV VSLAACSGGE KKASSDVS 1K

VDIEFISCDD

VNQDTFKWYA

FEKVMQQ IKE

WLKTFNDVYR

YIAIDGPSST

EGVTYAYNDQ

MQEWGKCKLV

KA.FLKSNKWD

DRYELDEKTP

SKLNAMISSC

LEDGKTYGYP

KYPEMTPMGF

ACIJISDDSFT

SGRKPTLNQT

GKIDYLPEVK

PHFVIENINP

AIEKIKSEKM

AWKLDKKKEP

DMPDIVTLTE

NYSN'TKADYE

TTVGDGAGPF

DDCATFDEKV

GISGWLSNY I

KLEQTDNDAY

DACTPEARAN

AENRDKLK

TKIKWYINSD

KTGQAALKAD

SGNI PVNDNF

LDKLQDFLGV

KQGNYATMLV

TKDAI(DPAKV

NKKYGISRFL

EAI ETKLNTT

WTALPFGKDV

SWAYSLUflLA

VIREDVYNAL

PLEDKNGKYY

AGTSGQGGNF

TQLFTLIDE

YFNNDRVNKL

VISMIRAKDD

TTAQIKKDLN

KKYDPYLMKV

GKPDVSTPEN

DRNLDKEYLE

TEFtMKKSGTR

PGQILTKYGV

KVPMESALTQ

KAFOKSLEDY

EF018-3 (SEQ ID NO:67) CTGTAG CGGTGGGGAA AACAAACCTA GCTCAGATGT GCTrrGGAAGT TAGATAAGAA TGGACGGCGC TGCCTTTTGG GTGGATATTG AATrTATrTC GATATGCCTG ATATCGTGAC TCTTGGGCCT ATTCTTrAAA GTTAACCAAG ATACGTTTAA AATTACTCTA ATACAAAAC GTTATTCGTG AAGATGTCTA TTTGAAAAAG TCATGCAACA CTCAA ITAAG

GAAAGAACCG

AAAAGACGTG

CGGCGATGAT

ATI'AACTGAA

CGATTTAGCT

ATGGTATCCC

GGATTATGAA

TAATGCATTA

GATI'AAAGAA

GATCGGTATG

ACCAAGATTA

ACCACTGCGC

TCAAANITrAA

AAAACTGGAC

AAAA.AATATG

TTAGAGGATG

AGTGGAAATA

GCCAAGCCAG

AAATATCCTIG

AATTAGATGA

AATGGTATAT

AGATTAAAAA

ATGCCATGAT

AAGCAGCATT

ACCCCTATTT

GAAAAACATA

TCCCAGTAAA

ACGTiI'CAAC

AGATGACCCC

AAAGACGCCT

TAACTCAGAT

AGACTTAAAT

TTCAAGTGGA

GAAAGCAGAT

AATGAAAGTT

TGGT1'AC CCT

TGATAATTTT

ACCAGAAAAT

AATGG~cTTC TABLE 1. Nucleotide and Amino Acid Seqeuenccs of E. faecalis Genes.

ACCACAGTCG

CCTTTAGAG

TGGTTAAAAA

GCAATrGGG

GCTCGGAACCA

TATATAGCCA

GGCATTTCAG

ACTCAACTGT

GAkAGGAGTTA

AAATTAGAAC

TACTTTAACA

ATGCAAGAAT

GATCCAGGAA

CT'TTCAA

AAAGCATTCT

GCGGAAAACA

GCGATGGTGC

ATAAAAATGG

CATTTAATGA

CAACGITTGCA

GTGG-TCAACG

TI'GATGGACC

GTTGGTTAAG

TCACATAT'T

CTTATGCGTA

AAACAGACAA

ACGACCGTGT

GGGGCAAAGG

CGCCGGAAGC

TGATTCGTGC

TAAAATCAA.A

GAGACAAACT

AGGACCATTT

TAAATACTAT

TGTTTACCGA

TCAAAAAGTG

TGGGAACTTC

AAGTAGCACT

TAATI'ACATT

AATTGAA

CAATrGATCAA

TGATGCCTAC

CAATA.ACTA

AAAATTAGTC

TCGTGCGAAT

GAAAGATGAT

TAAATGGGAT

TAAG

TTAGACAAA-l GATCGAAATr-

GCAGGCAATA

AAACAAGGAA

ACAGAATTTA

TCTGGCCGAA

ACGAAAGATG

CCGGGACAAA

GGAAAAATTG

A.ACAAkAAAAT

AAAGTACCAA

CCkATTTCG

GAAGCGA=T

A.AACCTCr GCAAT1'GAAA TACA.ACAC TI

TAGATAAAGA

TTAGTMATGA

ATTAT13CAAC

TGAAAAA.ATC

AACCAACATT

CGAA.AGATCC

TT'ITAACAAA

ATTA=ACC

ATGGCATT-AG

TGGAAAGT-GC

TAAT'MAAA

AAACCAAACT

ACAAA*TCTTT

A-AATAAA.ATC

CTTAGGTGTT

ATA=IAGAA

TAGCTTCACA

CATGCTCOTT

TGGCACACGT

AAATCAAACC

AGCAAAGTC

ATATGGCCTT

AGAAGTGAAA

TCCTTICCTA

'TTTAACGCAA

TATTAATCCA

AAATACAACC

AGAAGACTAC

TGAGAAAATC

EF018-4 (SEQ ID NO:68)

CSGGE

KKASSOVSIK

VDIEFISGOD

VNQDTFKWYA

FEKVNQQ IKE

WLKTFNDVYR

YIAIDGPSST

EGVTYAYNDQ

MQEWGKGKLV

KAFLKSNKWD

DRYELOEKTP

SKLNAIISSG

LEDGKTYGYP

KYPEMTPMGF

AGNISDDSFT

SGRKPTLNQT

GKIDYLPEVK

PHFVIENINP

AIEKIKSEKN

AWKLDKKKEP

DMPD IVTLTE

NYSNTKADYE

TTVGDGAGPF

DD)GATFDEKV

GISGWLSNYI

KLEQTDNDAY

DAGTPEARAN

AENRDKEA(

TKIKWYINSD

KTGQAALKAD

SGNIPVNDNF

LDKLQDFLGV

KQGNYATMLV

TKDAKDPAK(V

NKKYC-ISRFL

EAIE7TKLNTI'T

WTALPFGKDV

SWAYSLNDLA

\TIREDVYNAL

PLEDKNGKYY

AGTSGQGGNF

TQtjFTYLIDE

YFNNDRVNKL

VISMIRAKDD

TTAQ IKKDLN

KKYDPYLMI(V

GKPDVSTPEN

DRNLDKEYLE

TEFMKKSGTR

PGQILTKYGV

KVPMESALTQ

KAFDKSLEDY

EF019-1 (SEQ ID NO:69)

TAAAGGAGTT

CTI'GGTI'CAT

AAAACACATG

CGGATITATI'G

CAATGGACAG

CCCACTATT

TTAATCAGTT

CCAACTTATG

GCCACTGTTT

ACCAAAGGCG

TGGGTAACCA

TATCAGGACT

GCGGATI'GGA

CTTGTAAACA

GCTGTGAAAA

CCTAr1'GCCA

ACACAATGAA

TCTTACTCGC

AAGTAACAGA

CGAGTI'ATTT

TI'GGACAAGG

CCTATCACTT

CATCTCCTCT

TAGTCAAAAA

TAGATAAAAA

TCCAAGAATA

ACAACCAAGT

TAGGCCTCCA

ATCAAGTTI'C

GCGATGAATC

ATAACCAAGT

ATACTCAAAT

ACITI'TAAAA

AGCTI'GTGGT

TACCTTAGGC

AGAAGATTAT

CAGCAITCAA

GCCATATGAA

AGTI'GAAGGC

CGGCGAAAAT

AGAACAAGCG

TCITGCAAA

CTTIATGGTT

AGTTCCAAAA

TTI'AGAAAAA

AGCACCTCTT

TCATACCTAT

TGTTGAAGAT

AAGACGGTCC

AATACGAATA

AATAAAGTAA

CTAGTTGCAT

GATTATTTrAG

GCGGTTCTAA

GGTAAATACA

GTCACCTGGC

AAAAAAGTGT

AAAGATGCTG

AGCGATAATC

TTAGTGGAAG

TTAGCTGAGC

TTCCAAGAAG

GATAAAAAAA

GTAAAAAAAG

TAATTGGTAC

AAGAAGCCAA

CCGTCCCCGC

TAGGAGAAAA

CGAAAGAATT

AATTTGAACC

AAGAATACAG

GTGATCAAT

TAGA-AGATTA

GCAAATCTGC

GCTCAAGCGG

AAATI'TCTAA

TTGACGCAGA

CAATTTGGAA

GTAGTr'GGTT

CGCTC'IAAA

AACCCTTCTT

CAACGCTGAC

GAAACCCA.AA

ACCAGTCGCA

GAAAGATGTC

TGACTTATI'A

TAAAATTGCG

GGAAGATATT

TGATACCTTA

GGCAGTCTI'A

AACCGTGCTC

AAACGCTACT

CCACATTTI'C

GAACTTACCT

ATACAACGGA

TI'AA

EF019-2 ((SEQ ID NXLLKK TVLIGTI'LLL GSFLLAACGN TNKEANNADK THEVTDTLGN KV'IVPAKPKR IIASYLEDYL VALGEKPVAQ WTVGQGSIQD YLAK'ELKDVP TISYDLPYE-A VLKFEPDLLL 99 TABLE 1. Nucleotide and Amino Acid Seqeucnces of faecalis Genes.

ISSSALVECG

KGVQEYLGKK

DWNQVSLEKL

IANTQ IVEOV KYKEYSKIAP TYWVKNGENJV TWRDQLEDIA TVLDI<KEQAI( DAGKSAAVLW VTNNQVFMVS DNRSSGTVLY QDLGLQVPKL AELDADH IFL VNSDESAPLF Q.A IWXNLPA VKNNQVHTYD

KKALLN

KVLEDYDTLT

VEEI SKNATA

KKSSWLYNGP

EF019-3 (SEQ ID NO:71) TTGTGGT AATACGAATA AAGAAGCCAA CAACGCTC;AC

AAAACACATG

CGGATTATTG'

CAATGGACAG

CCCACTATTT

TTAATCAGTT

CCAACTTATG

GCCACTGTTT

ACCAAACGCG

TGGGTAACCA

TATCAGGACT

CGATTGGA

CTTGTAAACA

GCTGTGAAAA

CCTATTGCGA

AAGTAACAGA

CGAGTTATTT

TTGGACAAGG

CCTATGACTT

CATCTGCTCT

TAGTCAAAAA

TAGATAAAAA

TCCAAGAATA

ACAACCAAGT

TAGGCCTCCA

ATCAAGTTTC

GCGATCAATC

ATAACCAAGT

ATACTCAAAT

TACCI'AGGC

AGAAGATTAT

CAGCATTCAA

GCCATATGAA

AGTiGA.AGGC

CGGCGAAAAT

AGAACAAGCG

TCTTGGCAAA

CTTTATGGTT

AGTTCCAAAA

TITTAGAAAA.A

AGCACCTCTT

TCATACCTAT

TGTTGAAGAT

AATAAAGTAA CCGTCCCCGC CTAGTrTGCAT TAGGAGAAAA GATTATTTAG CGAAAGAATT GCGGTrCTAA

GGTAAATACA

GTCACCTGGC

AAAAAAGTGT

AAAGATGCTG

AGCGATAATC

TTAGT'GGAAG

TTAGCTGAC

TTCCAAGA-AG

GATAAAAAAA

GTAAAAAAAG

AATTTGAACC

AAGAATACAG

GTGMATC6AATT

TAGAAGATTA

GCA.AATCTGC

GCTCAAGCGG

A.AATTTCTAA

TTGACGCAGA

CA-ATTTGGAA

GTAGTTGGFI'

CGCTC'ITAAA

GAAACCCAAA

ACCAGTGGCA

GAAAGATGTC

TGACTTATTA

TAAAATTGCG

GGAAGATATT

TGATACCTTA

GGCAGTCTTA

AACCGTGCTC

AAACCCTACT

CCACATTTTC

GAACTTACCT

ATACAACGGA

T

9 *9 9* 9* 9 9 9 99999*

S

S*S

S

EF019-4 (SEQ ID NO:72) CGN TNKEANNADK THEVTDTLGN KVTVPA.KPKR IIASYLEDYL VALGEKPVAQ WTVGQGSIQD YLAKELKDVP ISSSALVEGG KYKEYSKIAP TYVVKNGENV rtWRDQLEDIA KGVQEYLGKK DAGKSAAVLW VTNNQVFMVS DNRSSGTVLY DWNQVSLEKL AELDADHIFL VNSDESAPLF QEAIWKNLPA IANTQIVEDV KKALLN

TISYDLPYE-A

TVLDKKEQAK

QD)LGLQVPKL

VKNNQVIITYD

VLKFEPDLLL

KVLEDYDTLT

VEEISKNATA

KYSSWLYNGP

EF020-1 (SEQ ID NO:73) S* S .5

TGAGGAGATG

ACATTAACTG

ATAATGAAAG

TATAAAGATA

ACTAGCGCAT

AGTAAAGTAA

GGGAAATFIA

AGAAAATGAA

CATGTAATGG

ATTCTCATA

AAGACACTAA

TGGAGTATTT

CC TTGATGA

AATAA

AAAGGTAGTT

TTCTAALATTA

TCGTGATGAA

TCGTTATTG

TTATTATTAT

TATGAAAGCT

TCAAT=GTT TGATGTGT GATAAAACAG GTGAAGAATT TATTCAGAAG ATG-GT'I=AG GCTGATTrr GGGTTCCTCT GATGAAGATA AGCGATTAGA AGTGGAAACT ATG-AAGTAGT

TCCAGTCTTC

TAAAAATTCT

TTTTTTAATA

TAAAGATGAA

TAGTACTAAA

GTATAAATcA EF020-2 (SEQ ID NO:74) NXKVVS ILLMVVAVFT LTACNGSKLD KTGEEFI(NSI MYtDSSYGDEY SEDCFSFLIY KDKDTNRYLA DVWVPVXDET SALEYFYYYD EDI(RLDSTKS KVTFDDMKAS GNYEvvYKSG

KFK

EF020-3 (SEQ ID ATGTAATGC TTCTAAA'ITA GATAAAACAG GTrAAGAATT TAAAAAITTC TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

ATAATGAA.AG ATTCTTCATA TGGTGATGAA TATTCAGAAC TATAAAGATA AAGACACTAA TCCTTA =TG CCATCTT= ACTAGCGCAT TGGAGTAT'r TTATTA'IrAT GATGAAGATA AGTAAAGTAA CCTTTGATGA TATGAA.AGCT AGTGGAAAC'r GGGAA.ATTTA AA

ATGGTTTTAG

GGGTTCCTCGT

AGCGATTAGA

ATGAAGTAGT

TT'ITTAATA

TAAAGATGAA

TAGTACTA.AA

GTATAAATCA

F-F020-4 (SEQ ID NO:76) CNGSKLD KTGEEFKNSI KDSSYGDEY SEOGFSFLIY KDKDTNRYLA DVWVPVKDET SALEYFYYYD EDKRLDSTI(S KVTFDDK(AS GNYEVVYKSG

KFK

EF021-1 (SEQ ID NO:77) *4 4 0* 4.

S 4 .4 4* 4 4 .5 5 5

S

4

S

TAGTTGITA

TI'ATTCG.GTT

GGCAAAGGCA

ATCATTACAG

TTGCAAGCTT

CAATCGAATG

AACACAATCT

AACCCTCATA

TCTGCAACAT

ACAAAAACGA

CAAGCTGGTT

GT'MATACCA

GCAATGTACC

GTCTTCCAAG

GGCGTTGACC

AACTTCACGT

CGTGCGTTAG

GGCGTTGACT

AAAGATAAAG

AATACATTAA

'ITAG IWTCAT

AAACCGCTGA

ATACAGGCGG

GGGGTAAAGA

ATGCAGCTGA

TTGGTATTGG

CAAACITI'GT

TTAGAGATAA

ACAAAGTCGG

TTGAAAAAGG

AATATCGC

AAAACGGCGT

AAGCAAAAGA

GCGATCAAGA

TAACTTCAAC

AAGACAAATT

TAACAGACGC

TAATCTCAGG

ACTATT'rrA

TCCATTAGGT

AAGCGGCGT

CGTGGATGAC

ACATGATTTA

CTATACAACC

CTACTI'GCTA

'ITTAATCGAT

TGAAGCAGCT

TTTTG'FrGGT

TGTGGCTGAT

T TCATTTGCT

TGATATCATC

CTTGAATGAA

TrCTGATGGC

GCITAA.AGGT

CCCTGGTGGC

CTATTTAAAC

TGACGTAAAA

GGAGGCTTTA CAGAAATGAA TTATCAGT'r CACITGCAGC GGCAAAGCGr ATGCAGCGCA A.AGTCGTTCA ACCAATCTTC CCAGAAGGTT CAAAA-GGGTA AATA'FTGACC AACCGTATC AAAGATGCAA TTTCTTCTGC GATCAAATCG. ATGCCAAAA TACTTAGCCG CTCTTC;CTGC GGTGAAGAAG GCTCGTAAT GCTGCGAAAG AA'11AGGTAA

AAAAGCAAAA

ATGTGGTCGGT

TAGTGCTCTA

TTGGGAAGGA

TGCATATATT

A.AGTAAATTC

AGCAGATGCC

GAATGTCGTT

TGCAAATGA-A

TGACCGTTTC

AGAAATTACT

TTTAGCTGCT

TGGACAAGGG

TTGGGTAATC

CAAAGAAGAC

TATTGCCAAC

AAAAGATGGT

TAAAACAGCA

GATCCTGCCA

TTCCATGCTT

TCAGGTTCTG

AAGTACAAAA

GTCGGCACAG

GAACATTTAG

GACAAAACAA

GTCCCAGAAA

AAGGGAAAGC

C'I'GCTGCGAC

GCGACIAAGT

CAAkAAGACGG

CGGTTCAAGA

TTTATGGATT

AAGAAGC 'GT

AACCAGAATA

*44* 4 EF021-2 (SEQ ID NO:78) MKKAKL FGFSLIALGL SVSLAACGGG KGKTAESGGG KGDAAHSAVI ITDTGCVDDK SFNQSSWEGL QAWCKEHDLP EGSKGYAY IQ SNDAADYTTN TIFGIGYLLK DAISSAADAN PDTNFVLIDD QIDGKKNVVS ATFRDNAAY KTNKVGFVGG EEGVVIDRFQ AGFEKGVADA AKELGKEIIV DTKYA.ASFAD MYQNGVDIIF MASGATGQGV FQEAXDL.NES GSGDKVWVIG VDRDQDAflGI FTLTSTLKGV GTAVQDIANR ALEDKFPGGE HLVYGLKDGG VDLTDGYLND DKVISGDViKV PEKPE

IDQAVSSKFN

LAG vAAANET

PAKGKALAAA

YX<TKDGKEDN

KTKEAVKTA-K

EF021-3 (SEQ ID NO:79)

ATGTGGTGGT

GGCAAAGGCA AAACCGCTGA AAGCGGCGGT GCAAAGrGG ATGCAGCGCA ATCATTACAG ATACAGGCGG CGTr.GATGAC AAGTCGTTCA ACCAATCTTC TTGCAAGCTT GGGGTAAAGA ACATGATTTA ccAGAAGr CAAAAGGGTA CAATCGAATG ATGCAGCTGA CTATACAACC AATATTGACC ACGGTATC

TAGTGCTCGTA

?I'GGGAAGGA

TGCATATATT

AAGTAAATTC

101 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. facalis Genes.

AACACAATCT

AACCCTGATA

TCTGCAACAT

ACA.AAAACGA

CAAGCTGGTT

GTTGATACGA

CCAATGTACC

GTCTTCCAAG

GGCGT'rGACC

AACTTCACGT

CGTGCGTTAG

GGCGrrGACT

AAAGATAAAG

TTGGTATTGG

CAAACTT

TTAGAGATAA

ACAAAGTCGG

TTG-rAAAAAGG

AATATGCGGC

AAAACGGCGT

AAGCAAAAGA

GCGATCAAGA

TAACTTCAAC

AAGACAAA T

TAACAGACGG

TAATCTCAGG

CTACTTGCTA

FI'TAATCGAT

TGAAGCAGCT

TTTTTTGGT

TGTGGCTGAT

TTCATTTGCT

TGATATCATC

CTTGA-ATGAA

TGCTGATGGC

GCTTAAAGGT

CCCMGMGC

CTA I=AAAC

TGACGTAAAA

AAAGATGCAA

GATCAAATCG

TACTTAGCCG

GGTGAAGAAG

GCTGCGAAAG

GATCCTGCCA

TTCCATGCTT

TCAGGTTCTG

AAGTACAAAA

GTCGGCACAG

GAACATTrAG G6ACAAAACAA

GTCCCAGAAA

TTTCTTCTrGC

ATGGCAAAAA

GTGTT-ccTGc

GGGTCGTAAT

AATTAGGTAA

A.AGGGAAAGC

CTGGTGCGAC

GCGACAAAGT

CAAAAGACCG

CGGTTCAAGA

TTTATGGATT

AAGAAGCTGT

AACCAGAA

AGCAGATGCC

GAATGTCGTT

TGCAAATGAA

TGACCGTTTC

AGAAATTACT

TTI'AGCTGCT

TGGACAAGGG

'ITGGGTAATC

CAAAGAAGAC

TATIGCCALAC

AAAAGATGGT

TAAAACAGCA

EF021-4 (SEQ ID 6O

S.

S S 8S Sb

S

55

S.

S

S

S.

S S 555 0

S

S

P.S.

5.5.

5 5

S

S.

S.

CGGG KGKTAE-SGGG KGDAAHSAVI ITDTGGVDDK SFNQSSWEGL QAWGKEHDLP TIFGICYLL( DAISSAADAN PDTNFVLIDD KTNKVGFVGG EEGVVIDRFQ AGFEKGVADA MYQNGVDIIF HASGATGQGV FQEAKDLNES FTLTSTLKGV GTAVQDIANR ALEDKFPGGE DKVISGDVKV PEKPE

EGSKGYAYIQ

Q IDGKKNVVS

AKELGKEITV

GSGDKVWVIG

HLVYGLKDrG

SNDAADYTTN

ATFRDNEAAY

DTKYAASFAD

VDRE)QDADGK

VDLTDGYLND

IDQAVSSKFN

LAG VAAANET

PA.KGKALAAA

YKTKDCKEDN

KTKEAVKTAK

E-7022-1 (SEQ ID NO:81) TAAGAGCATA AAAA.AATGAA ACAATGG ITr GTATTTTATTI

AAAAAGAAAC

ACGCTCAACA

GAAGGGTTAT

CCGATGAT

AGTAACGATG

CCTAAAAACG

ATICTCAGCGG

TTrAAAGGTGA

TFITCCCGC

GATAAAGT'CG

TGGCAACTAG

AATTATACAG

GATG.TrGCTA

TCGTATCCAA

ACGCCGCTTG

CTAGTCAATA

TTI'GTGGCGA

TATAACAAAG

GTTAACGrI'G

CAAGGCTCGC

GAAGCTGCAT

CCAGACTATC

TATCAGAACC

CCAGAAAAA.A

AGAAAAATAC

CCTCTGTAT

ATACTTTAGA

CAGAAGATGG

ATCCTGTCAC

GC=TGTTTA

GGAAATTAGC

CGCTCAAAGA

AAAATCNAAA

TCTATAATGG

CAAAAAATAA

T1TATCAAAGA

CACTAAGTGG

CAGCGACAAT

CAAACGAAAA

ATATTA'FrGC

ATCCCACAAC

AAAAAGCGCA

AA'TGrATGGT

TACAAGAATT

TGAACTTTGG

AAGACCCTAT

CTGTCTATGA

GATGGGCGAC

GAGTTATAGG

GGTAGGATTT

CAAAGAAGCC

ATTGGAT TT

TGAACAAGAC

AAAAACCTAC

AGCACATGAT

TAGCTCCTC

ACCCAATGAA

GCCAAAACCG

AGTAGTCGAA

TCCGTTCGTG

TCGCTATTGG

AACATCTACC

TGAACTGGCG

GAACTATTTG

CCTGCGTAAA

AGATGGTTCT

GGGTCTCGAT

AAGTTATTGG

AACAGATGAT

GTTTCCTGGT

GCGAGAAAGT

TTCTACCCTG

CAAA ITA TA

ACTGATTGCA

AGAAAGAAGA

TAGCTGGGT

GTTCAACTGA

CCAGATGCTA

CAATTGGTAC

ACGATTTC=

T TTGAATATG ATCGTrGAA

CTAGGTGTCA

TACTTTACGT

CAATTTGG'?G

GTAAAAGATT

GATCACCACA

GCATTGAATC

CAACAGAATIA

CGCTTAA.ATC

GCA'PTGGCTT

AAAGCGCTAC

TTTCGTCAAG

AAAAAAGCAc

GG'ITCTTACA

=TACAATAG3

GACTATGATT

ATGACTTT

GATGAAGCAG3

GCTGAAAAAG

TGAAAAAGTA

GTACCAATAA

TGTCACCCTC

TIGTCCAAAC

CAGCCGTAGC

TCAGAAAAGA

CTTGGAAAAA

CAA~rCAAAA

CAGCTGTGGA

CC7"lrTTAGC

CGGACTATGG

GGCAGCAAAC

ACGTGCGCTC

TTT=rAAGA

AAAATAATAC

AAAA.ACGGNA

TAGGAATAGA

ATGGTGCGAT

AAGCAGGTAA

AAGCAGAATT

AAAAAAPTGG

AGCTAACCGC

TATTCTTAAT

ACAkAGGGCAA

CCACAACCTA

AAGTIGA'ITGA

TI'TAAAAATC

AAATGAAAAT

GGAATTAACA

TGCAGCGTTT

AAAAGCATTG

AGC-GCTITTG

AATGATTGAT

TGGTGCAGAA

TGATTATACA

TITTCCGACA

AACTGCTAGT

AAAGATGGAC

AGACATTATC

TGGACAATI'A

GTTGTATCAT

AGGGCAAGCN

TAAAGAAAAT

TACGGAAGGC

TTTAATGGTT

AGGAGAAAAG

TGAAAGTTTG

ATTGCCGACT

TTACTGGACA

TGATCGCAAT

TGCCTTAGAG

AACGACTGCT

102 TABLE 1. Nucleotidc and Amino Acid Seqeuence-s or E aecalis Genes.

GGCATCATTC CACTTAGCCA AAATGAACAA ACAGTCCTGC AAAATGATAA AGTCAAACGC TTGAATTTTC ATACCTTTCG CGCTCCATTA ACGTTAAAAA ATGTTI'ATAA GGAAAAATAA EF022-2 (SEQ ID NO:82) M4KIYLR IT M4VC ILLVGFL AGCTNKNENK KKQKNiTKEAV QLMSPSELIT

LNTSVLLDFP

NODPVTAHDF

KVTLKEPKPY

QLAKNNRYWD

YPTATMNYLR

VANPTTGLDF

GSLQELFPGL

QN~PVYDKLLD

NFHTFGAPLT

DAIVQTAAPE

EYAWKKM ID P

FTSLLAFPTF

HQNVRSDIIN

LNQKRXGQAT

RQEAGNLMVY

TIELTALPTE

EAATTYALEP

LKNVYKEK

GLYSLOEQDQ

KNGFVYSFLI

FPQNXKVVEQ

YTVIKETSTA

PLANENLRKA

NKEYXAQSYWK

AALNFGRESD

EKRWATLIAA

LVPAVAKALP

VETIQNGAEI

FGADYGTASD

LNLFEDGQLD

L.ALC IDKE.NL

KAQAELGEXV

YDLFLIYWTP

EKEVIETTAG

MISEDCKTYT

SAGKLAPNEL

KVVv.NGP'.VV

VATLSGELAQ

VNNI IA DCS K

NVELMVTEDDG

DYQDP ISTLM

MIPLSQNEQT

ISLRKE-AVWS

GVTAVIDDYTL

KDWQQTKMDW

QNKNNTLYHS

ALHGAITEGF

SYKKIGESLQ

TLYKGNDRNY

VLQNDKVIKCL

S.

6@

S@

S.

SO S

*O

OS

S 0

S.

0@ S 0

S

S.

5 S OS. S 0

S

0

S.

0550

S

0 *05S

S

0S50 00 0

S.

56 EF022-3 (SEQ ID NO:83) GT GTACCAATAA AAATGAAAAT

AAAAAGAAAC

ACGCTCAACA

GAAGGGTTAT

CCGATGATTT

AGTAACGATG

CCTAAAAACG

ATCTCAGCGG

TTAAAGGTGA

TTTTTCCCGC

GATAAAGTCG

TGGCAACTAG

AATTATACAG

GATGTCCCTA

TCGTATCCAA

ACGCCGCTTG

CTAGTCAATA

ITTTGTGGCGA

TATAACAAAG

GTTAACGTTG

CAAGGCTCGC

GAAGCTGCAT

CCAGACTATC

TATCAGAACC

CCAGAAAAAA

GGCATGATTC

TTGAATTTTC

AGAAAAATAC

CCTCTGTATT

ATAG=TAGA

CAGAAGATGG

ATCCTGTCAC

GCT'ITTrTA

GGAAATTAGC

CGCTCAAAGA

AAAATCNAAA

TCTATAATCG

CAAAAAATAA

TTATCAAAGA

CACTAAGTCG

CAGCGACAAT

CAAACGAAAA

ATA ITATTC

ATCCCACAAC

AAAAAGCGCA

AATTGATGGT

TACAAGAATTr

TGAACTTTGG

AAGACCCTAT

CTGTCTATCA

GATGGGCGAC

CACTrAGCCA

ATACCTTTGG

CAAAGAAGCC

ATTGGAT=

TGAACAACAC

AAAAACCTAC

AGCACATGAT

TAGCCTCCTC

ACCCAATGAA

GCCAAAACCG

AGTAGTCGAA

TCCGTTCGTG

TCGCTATTGG

AACATCTACC

TGAACTGGCG

GAACTATTIrG

CCTGCGTAAA

AGATGGTTCT

GGGTCTCGAT

AAGTTATTGG

AACAGATGAT

GTTTCCTGGT

GCGAGAAAGT

TTCTACCCTG

CAAATTATTA

ACTGATTGCA

AAATGAACAA

CGCTCCATTA

GTTCAACTGA

CCAGATGCTA

CAATrGGTAC

ACGATITCT

TTTGAATATG

ATCGTTGAAA

CTAGGTGTdA

TACTTTACGT

CAATTTGGTG

GTAAAAGATT

GATCACCAGA

GCATTGAATC

CAACAGAATA

CGCTTAAATC

GCATTGGCTT

AAAGCGCTAC

TTTCGTCAAG

AAA.AAAGCAC

GGTTCTTACA

TGACAATAG

GACTATGATT

ATGACTTTAT

GATGAAG CAG

GCTGAAAAAG

ACAGTCCTGC

ACCTTAAAAA

TGTCACCCTC

TI'CTCCAAAC

CAGCCGTAC

TGAGAAAAGA

CT'-GGAAAAA

CAATI'CAAAA

CAGCTGTGOA

CCTTGITAGC

CGGACTATGG

GCCCAAAC

ACGTGCGCTC

T'TTTGAAGA

AAAATAATAC

AAAAACGGNA

TAGGAATAGA

ATGGTGCGAT

AAGCAGGTAA

AAGCAGAA 1T

AAAAAATTGG

AGCTAACCC

TATTCTTAAT

ACAAGGGCA.A

CCACAACCTA

AAGTGATTGA

AAAATGATAA

ATGTI'TATAA

GGAATTAACA

TGCAGCGIT

AAAAGCA'ITG

AGCGGTTTCG

AATGATTGAT

TGGTGCAGAA

TGATTATACA

TTI'TCCGACA

AACTGCTAGT

AAAGATGGAC

AGACATTATC

TGGACAAITA

GTTGTATCAT

AGGGCAAGCN

TAA.AGAAAAT

TACGGAAGGC

TTTA.ATGGTT

AGGAGAAAAG

TGAAAGT'ITG

ATTCCOACT

TTACTGGACA

TGATCGCAAT

TGCCTTAGAG

AACGACTGCT

AGTCAAAGGC

GGAAAA.A

EF022-4 (SEQ ID NO:84) CTNKNENX KKQKNTKEAV QLMS PSELTT LNTSVLLDFP DAIVQTAAFE GLYSLDEQDQ LVPAVAKALP M7ISEDGKTYT ISLRKEAVWS NDDPVTAHDF EYAWKKMIDP KNGFVYSFLI =EIQNCAEI SAGKLAPNEL GVTAVDDYTL TABLE 1. Nucleotide and Amino Acid Seqeuences of E, faecalis Genes.

VTLKEPKPY FTSLLAFPTF QLk-KNNRYWO HQN'VRSDI IN YPTATMNYLR LNQKRXGQAT VANPTTGLDF RQEAGNLI'VY GSLQELFPGL TIELTALPTE QNPVYDKLLD EAATTYALEP NFHTFGAPLT LXNVYKEK

FPQNXKVVEQ

YTVIKETSTA

PLANENLRKA

NKEKAQSYTWI

AALNFGRESO

EKRWATL IAA

FGADYGTASD

LNLFE)GQIJD

rJALG I DENIJ

KAQAELGEKV

YDLFL IYWTP EKEVI ETTAG

KVVYNCPFWV

\IATLSGELAQ

VNNI!IADGSK

.IVELMVTDDG

DYQDP ISTUL1

MIPLSQNEQT

KDWQQTKM]DW

QNKNNTLY14S

ALHGAITEGF

SYKKIGESLQ

TLYKCNDRNY

VLQNIDKVKGL

EF023-1 (SEQ ID 0 4J

TAAAATGGAG

GC=tIAACGG

CAAAAAATTG

GATACGACCT

GAAGATGAAT

GTTATACAT

A.ACGACI'TTG

GCTTTATTTT

AATACTCTTG

ACCCCTTATT

ATCAAAGAAC

TTTAAATTGA

ACGTATTGGG

CCAACGACAG

GGTGAATTTA

ACATAC

4

TTT

AACAATATTC

TTGCAAGATC

GATGGAACAG

GCGAAAGCAA

TTAGAA'ITTTT

CATTGCAAG

CGTGTTGATC

TATCGTGATC

ACGT'TCAAGA

GATCAAGAGG

ACGGITTTAG

CATCTCTATT

GGATCGGTAT

CTTGTCAGGC

CAATTAGTTC

CGACTTTAGT

TAGAGTTGGG

TTAAGATTAG

TATOGCATG

TTGATGOTCAT

GGGTTAAGGC

TGAAATCATT

AAGGGGATAA

AAGAATGGGA

ATGCTAAAA

CGGTGAA'IT

TTCCTGG'FrA

TAAAAATGAA

GTAAAGCGT

AATCAACGGC

AT1'TCACAAA

AAGAATTCTG

TAACAGATGA

AAAATCTAGA

GTGATCAAAC

CATTAACAGT

GTCATACGTA

CTCGTTTAAA

CACCAATCTA

OGCATTCAIT

GAAGAAATTA AAAATGTTAG GGGAACGGGA' AACTCGGCTG TGAAGCCGCT ATTTCGACAA CATCAATCAA GTTTATGAAG GGTCGCTGCC GAAGAACCAG AGAAGATGCC AAATGGTCGA GCAACAAGTT GCTTCCCCTA TAAAAATGCT AAGGAAATTG GCTAGATGAT AAAACGTTAG AC=TCCGF= CCTGTTTTGT ATATGCTACT GATGCAGAAC TAATGCCTCT TCTGATGACT AGTTAAATTA ACAGAAGCGA GITGACTCG AATGAATGG TGTI'GATAAT CCAGCC C CAGCGTTCGT GATGGAAAAG GGCACAACCT TTTGATAAAG TACAGATCA-A GTAATTCCGC ACTAGCTGCT AAGAAAAATA GGAAAAAGGG AAAAAAGAAA TACAGACAGC GCCAAAAAAG TGGATTAGAA GTGAATGTTA CAGACACTAT GAITTAGAAT TATGCGCATC TTTACTTCGG TGATCAATTA AI'CAAGAAA TGAC TrCCT CAACCACAAG CAATCGAAGC ATTTCTGTAT TGGACCCACG TACAGTTTAA

CATGCGTCCG

ATAGTA.ACAA

TGGAACCACA

GAG TcirATGT

CGATTTCTGA

ATGATGATCC

AATCAGGATC

CTTTAGAACGr

AAATAACTT

'ITCCACAAAA

AT=IATTTA

GGACCTACGA

AAGTTTCAGT

ATGTAGTGA

TTTCAATTCC

AAAATCCGGC

AAAGTTT

CGGGACAAAC

ACTACTTAAC

TI'GGGCTGGA

CTGCTGAGTT

CTCAAGTTCC

TATCTGGTTG

ATAGTACC TT

CTAGAACAAC

ATATITTGGT

TAGCTAATCA

AATGGGCTTA

GTTGCTTTTA

AGCAGCGGAA

CACAGCGGGG

TTI'AGGTAAA

AGATGAA.ACC

AGTAACAGCA

GATTCATCAA

CGCAGATGTG

AGAACGGCCC

TGAAAAATAT

TAATGGTCCT

AAAAAATGAT

AATTAAGAGC

TAAGCTAAGT

TCAATTCGTC

ITAGCGAAC

AAAAGAAGTC

GATTGCGCCA

CTACGATACA

TA-AAATCAAA

TTTCCAATT

TI=TACTATT

GGGAACCGAT

GGGCGGCGTA

ACATGCGGCT

GAATCAGGAA

AAAAATCAAG

TGTTAACTAA

EF'023-2 (SEQ ID NO:86) MKKLK MLGCVGLLLA LTACQAGTGN SADSNKAAEQ KIAISSEAAI STMEPH-TAGD

TTSTLVMNQV

DFVYAWQQVA

PYLKSLLSFP

YWDAEKVKLT

YFLKMNSVRD

GTDFTKLAAK

LEENLDGLEV

FKSDTYDQLI

LYWHSFGPTY

YEGLYVLGKE

SPKSGS I}QA VLFPQNEKY I

EAKVSVIKSP

GKENPALANN

KNNYLTYDTA

NVTQVPFTIR

QETRTTHAAD

SLKWAYVN

DELELGVAAE

LFFDVIKNA(

KEQGDKYATD

TTAVNLFDSN

NIRKALAQAF

KAKEFWEKGK

VDRDQTRDYD

QEARLNDFAQ

EPAISEDETV

EIALEGADVN

AEHLIYNGPF

ELDVVNKLSG

DKESFVKEVL

KE IGLDKI(L

LELSGWGTDY

AQDILVNQET

YTFKI REDAK

TLGVKALDDK

KL.KEWDNASS

EFIPGYVDNP

QDQSTATDQV

EFLTDDTDSA

RDP LTVMR IF VtLAPIYNRSI

WSNDDPVTAN

TLEITLERPT

DDWTYEKNDT

AFLSIPQFVT

IPPGQTIAPD

KXAAEFFQFQ

TSDSTLGGVT

SVLANQKIKD

TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

EF023-3 (SEQ ID NO:87)

GGGAACOGGA

CAAAAAATTG

GATACGACCT

GAAGATGAAT

GT'ITATACAT

AACGACTFI'G

GCTTTATTTT

AATACTCTTG

ACCCCTTATT

ATCAAAGAAC

TTTAAAITGA

ACGTATTGG

CCAACGACAG

GGTGAATTTA

ACATACTr'rr

AACAATATTC

TTGCAAGATC

CATCGAACAG

CCGAAAGCAA

TTAGATr CAA'FrCGAAG

CGTGTTCAT'C

TATCGTGATC

ACCTTCAAGA

GATCAAGAGG

ACGGTTTTAG

GATCTGTATTr

AACTCGGCTG

CAATTAC ITC

CGACTTTAGT

TAGAGTTGGG

TTAAGATTAG

TTTATGCATG

TTGATGTCAT

GGGTTAAGC

TGAAATCATT

AAGGGGATAA

AAGAATGGGA

ATGCTGAAAA

CGGTGAATTT

TTCCTGG'ITA

TAAAAATGAA

GTAAAGCGTT

AATCAACGGC

ATTTCACAAA

AAGAATTCTG

TAACAGATGA

AAAATCTAGA

GTGATCAAAC

CAFI'AACAGT

GTGATACGTA

CTCGTTTAAA

CACCAATCTA

GGCATTCATT

ATAGTAACAA

TGAAGCGGCT

CATCAATCAA

GGTCGCTGCC

AGAAGATGCC

GCAACAAGTT

TAAAAATGCT

GCTAGATGAT

ACTTTCGTTT

ATATGCTACT

TAATGCCTCT

AGTTAAAT'rA

GTTTGACTCG

TGTTGATAAT

CAGCGTTCGT

GGCACAAGCT

TACAGATCAA

ACTAGCTGCT

GGAAAAAGG

TACAGACAGC

TGGATTAGAA

GAGAGACTAT

TATGCGCATC

TGATCAATTA

TGACTTTGCT

CAATCGAAGC

TGGACCCACG

AGCAGCGGAA

ATTTCGACAA

G TrATCAAG

GAAGAACCAG

AAATGGTCGA

GCTrCCCCTA

AAGGAAATTG

AAAACGTTAG

CCTG71rTGT 5ATGCAGAAC

TCTGATGACT

ACAGAAGCGA

AATGAATT1GG CCAGCCMtTC

GATGGAAAAG

TTCATAAAG

GTAATTCCGC

AAGAAAAATA

AA.AAAAGAAA

GCCAAAAAAG

GTGAATGTTA

GATIAGAAT

TTTACTTCGG

A'FrCAAGAA.A

CAAGCACAAG

AT=CTGTAT

TACAGTTTAA

TGGAACCACA

GACTCTATGT

CGATI'TCTGA

ATGATGATCC

AATCAGGATC

CTTTAGAAGG

AAATAACTTT

TTCCACAAA.A

ATTI'ATTTA

GGACCTACGA

AAG'FrTCAGT

ATGTAGTGAA

TTTCAATTCC

AAAATCCOGC

AAAGTTITT

CGGGACAMA.C

ACTACTTAAC

TTGGGCTGGA

C'-GCTGAGT

CTCAAGTCC

TATCTGG'ITG

ATAGTACCTT

CTAGAACAAC

ATATGGT

TAGCTAATCA

AATGGGCTTA

CACAGCGGGG

TTTAGGTAAA

AGATGAAACC

AGTAACACCA

GATTCATCAA

CGCAGATGTC

AGAACGGCC

TGAAAAATAT

TAATGGTCCT

AAAAAATGAT

AATTAAGAGC

TAAGCTAAGT

TCA.ATTCGTC

TTTAGCGAAC

AAAAGAAGTC

GATTGCGCCA

CTACGATACA

TAAAATCAAA

TTTCCAATTT

TTTTACTAFr

GGGA.ACCCAT

GGGCGGCGTA

ACATGCGGCT

GAATCAGGAA

AAAAATCAAG

TITTAAC

EF023-4 (SEQ ID NO:88) GTGN SADSNKAAEQ KIAISSEAAI STMEPHTAGD

TI'STLVMNQV

DFVYAWQQVA

PYLKSLLSFP

YWDAEKVKLT

YFLKMNSVRD

GTDFTKLAAK

LEENLDGLEV

FKSDTYDQLI

YEGLJYVLGKE

SPKSGSIHQA

VLFPQNEKYI

EAKVSVIKSP

GKENPALANN

KNNYLTYDTA

NVTQVPFTIR

QETRTTHAAD

DELELGVAAE

LPFDVIKNA<

KEQGDKYATO

TTAVNLFDSN

NIRKALAQAF

KAKEFWEKGK

VDRDQTRDYD

QEARLNDFAQ

EPAISEDETV

EIALEGADVN

AEHLIYNGPF

ELDVVNKLSG

DKESFVKEVL

KEIGLDKIKL

LEL

1

SGWGTDY

AQDILVNQET

YTFKIREDAI(

TLGVKALDDK

KLKEWDNASS

EF IPGYVDNP

QDQSTATDQV

EFLTDDTDSA

RDPLTVMRIF

VLAPIYNRSI

WSNDDPVTAN

TLEITLERPT

EDDWTYEKNDT

AFLS IPQFVT

IPPGQTIAPD

KKAAEFFQFQ

TSDSTLGGVT

svLANQKIKJ LYWHSFGPTY SLKWAYVN EF024-1 (SEQ ID NO:89)

TAATGGCCGT

AACAAGGATC

GTCCGGCTTGT

GGTGGTAAAT

TTCAGCTA

AAAAAAATAA

AANGNTANCA

ACAGAATAA

TTCGTCTACT

ATAAAAAAGG

TATTGT'GTC

GGAAAGTGGA

ATGACTCAGA

CCrI'TGACNT

AGATTACAGG

AATAAAGAGG

AGAAGTGAGC

AGGTTGTGGA

AGAAACACGT

GGATAGTGTT~

TACTAGCAGN

GGAAATTGC

ATGAAGCTAC

ATGAAAAAAG

ACAGATATGA

GCAACTTACA

AGTGGGACAT

AACTCTTTCA

GAAAAACA.AA

TCAAATGGCG

TACTACC'TTT

AAAAGATATT

CTITTTTTGA

ACACTTATGA

TTATGGAAAA

GA.ACACTTAT

TI'GGCAATGG

TATTGCCTTA

GACTGCCGAT

TGACGGTAAA

TGAAAAAAAT

AGTNGANTNC

AAAACAAAAA

105 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

EF024-2 (SEQ ID M1 KKVLPFIALV GLLLLSGCGT DIMflKILTADG GKWKVEETRA TYTFFDDGKF SANDSEDSVS GTYTYDEKNK KITFDXTSXN SFIMEKVXXX XXKITGEICE KQRTLIKQKT E EF024-3 (SEQ ID NO:91) ATr GACTGCCGAT GGTGGTAAAT GGAAAGTGGA AGAAACACGT TTICACCTA ATGACTCAGA GGATAGTGTT AAAAAAATAA CC GACNT TACTAGCAGN AANGNTANCA AGATI'ACAGG GGAAATTGGC

ACAGAA

GCAACTTACA

AGTGGGACAT

AACTCT?1'CA

GAAAAACAAA

CTT'=TTGA

ACAC'tTATrGA

TTATGAAAA

GAACACTTAT

TGACGGTAAA

TGAAAAAAAT

AGTNGANTNC

AAAACAAAAA

55 S S EF024-4 (SEQ ID NO:92)

LTADG

GKWKVEETRA TYTFFDDGKF SANDSEDSVS GTYTYDEKNK KITFDXTSXN SFIMEKVXXX XXXITGEItE KQRTLII(QKT E EF025-1 (SEQ ID NO:93)

TGAATGAAAC

GCGCCAGATC

AACCAACGITA

AATGACGAAC

C'FrACAGGG

GAAAAAAAGA

TATI'ATAAAA

GAACCTGAAA

TATGATACAC

AGCAACACGC

AAACAAATGC

ACTTCTGCGC

CCATCGAAAG

CTTCAAGT3A

GATGAACAGG

ATTCACCAGG

ATATTAA-AGG

AAGAGCCAAC

CCGTCACCAA

TGATTGCTAA

CCACACAAAC

AAAA-AATGTT

ATGAAGGTGT

CGCAAAGGAT

GCTATI'CGC

GTACAGACGA

GTGACGAAAA

GTCAAGATTI

AAACGTATAT

TAACAGAAGA

AAAAAATCAC

ATACAATAAA

AATGTTGGTT

GAAACAAACA

TCAAACGACT

TCAATTGACT

GACATTTrGGA

TTGGTCCAAT

ATTTACTC

TCTGAATGTT

TGTCAACAAA

TACC'ITAGTC

TCGTGTTACA

AATGCCTTTA

CGGTTACGCA

GCAGAAAATA

AGAAACAGCC

CAAACCAACA

TTTATCGGAT

ACAAGTGGTC

TCTGCCGTCO

TTTGATTCTC

ACAACCCCAC

CAACCCCCTT

GCAAATTACG

GAGITTACAG

CCCTGTCGG

ACCGITGTTA

GGTAATT=~

GCAGCAGAGA

GAAGA11'TAG

AAACATGTCA

TGCGGCCT'T

ATTCTTTTAT

TATTTATTI

CGCAAGAGAC

AAAAACA.AGC

ATGAATACAC

CAGCAAAATA

TGGGATTAAT

GCATTG.TAGA

AGTTTGCTAG

ArrATCCTGA

ATC"GTATTAC

ATACGGTACG

TGGACACITG

CCAATGGCCT

GCTATGATCA

'rrATCGTCAA TCAT'FIT TA GC?1'CCTTGT

AAAGCAAGTG

GCCGACTAAA

GTACGAAGTG

TACACCGGAA

GACGGGTAAC

GATTATTACG

TGATCCI'AT

ATTCAAGCA

TTATGTAGAA

CGGTTCATCA

GCTAA.AAGAG

AATCGCTCGA

ATACTrrTCA TCGAA.ATA1T

G

EF025-2 (SEQ ID NO:94) MrKH IKGMLVF DEL IANQLTF

YKN'EGVFTCO

NTRTDDTLVT

SKETYIGYKE

HQDTINKPTI

IGUFILVGCA

DSHEYTYEVV

NYGIVEI ITE VVNG ITYVEK

DLGNGLIARL

LLFIL

PDQEPTKQTT

TGATQTTFGT

PETQRILNVE

QMRDENRVTG

QVITEEQKIK

SGPQETKQVK

TPPAKYTPEE

FT'EFASDPYY

NFY'IVRGSST

HVSYDEYFSD

QV'IVrNQTTS

KKMMFWSNQ

DTRYSGVNKR

SAREGLMPLA

EQEKITETAC

AVEKQAPTKN

PPLGLMTGNY

LSDYPEFQAS

AEMDI'WLKEP

GLFIVNRNI I EF025-3 (SEQ ID 106 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. facalis Genes.

AAC GAAACAAACA ACAAGTGGTC CGCAAGAGAC AAAGCAAGTG

AAGCAAGTTA

AATCACGAAC

G~rACAGGGG

GAAAAAAAGA

TATTATAAAA

GAACCTGAAA

TATGATACAC

AGCAkACACC

AAACAAATGC

ACTTCTGCGC

CCATCCAAAG

CT*TCAAGTGA

GATGAACAGG

ATTCACCAGG

CCGTCACCAA

TGATTGCTAA

CCACACAAAC

AAAAAATGTT

ATGAAGGTGT

CGCAAAGGAT

GCTATTCGGG

GTACAGACGA

GTGACCAAAA

GTGAAGGATT

AA.ACGTATAT

TAACACAAGA

AAAAAATCAC

ATACAATAAA

TCAAACGACT

TCAATTGACT

GACATTTGGA

'ITGGTCCAAT

ATTTACTGC

TCTGAATGTT

TGTCAACAAA

TACGTTAGTC

TCGTGTTACA

AATGCCTTTA

CGGTTACGCA

GCAGAAAATA

AGAAACAGCC

CAAACCAACA

TCTGCGGTGG

TTTGAJTrCTC

ACA.ACCCCAC

CAACCGCcTT

GGAAATTACG

GACTTTACAG

CGCCTGTCGG

ACCGTTrGTTA

GGTAATTTTI'

GCAGCCAGAGA

GAAGATTTAG

AAACATGTCA

TGCGGCC TT

ATTCTTTTAT

AAAAACAAGC

ATGAATACAC

CAGCAAAATA

TGGGATTAAT

GCATTGTAGA

AGTTTCTAG

ATI'ATCCTGA

ATGGTATTAC

ATACCCTACG

TGGACACTI'G

GCAATGGCCT

GCTATG'ATGA

ITATCGTCAA

TCAWT=

GCCGACTAAA

GTACGAAGTG

TACACCGGAA

GACGGGTAAC

GATTATTACG

TGATCCTTAT

AT'rTCAAGCA

TTATGTAGAA

CGGTTCATCA

GCTAAAAGAG

AATCGCTCGA

ATAC=TTCA

TCGAAATA'T

EF025-4 (SEQ ID NO:96).

a.

a.

a TKQT SGPQETKQVK QVTVTNQTTS AVEKQAPTKN DEL IANQLTF DSHEYTYEVV TGATQITFGT TPPAKYTPEE YKNEGVFTGG NYGIVEIITE PETQRILNVE FTEFASDPYY NTRTDDTLVT VVNGITYVEK QMRDENRVTG NFYTVRGSST SKETYIGYAE DLGNGLIARL QVITEEQKIK HVSYDEYFSD HQDTINKPTI LLFIL

KKKMFWSNQ

DTRYSGVNKR

SAREGLMPLA

EQEK ITETAC P PLC LMTGNY

LSDYPEFQAS

AEMDTWL.KEP

CUP IVNRNI I EF026-1 (SEQ ID NO:97)

TGAGTCTATG

ITTTTGGAG

CCTGCTCTTG

AGCAAACAAA

CCTGAAGAC

AATGTCACTT

TCCAACCATG

GCTGAATATG

CACGGGACTG

AAAGIAATC

AAAGATGGTG

ATTACTCATT

GGAT=ICAT

TGTTGCTTAG

CACCTAAITC

TCCAAATGC

TTGATAAAGA

TCTATCCTAA

AAATCACGGT

TCAAAGGTGA

GCAATAITAC

CCACTCTTAC

TCCCTTTGAA

GAAAATGTCT

TGCTTGTTCA

AACTACAGAA

CTTAACTCAT

GGTAACAGTT

ATTAGCACTT

TCCTAAGCTA

TATTGAGGTC

TTTTGATAAA

TCCTCAAGTC

TCAGTTATGA

AAAGTACTCA

TCT'GATAAAA

GTAGTCTCTG

A.AACCAAATT

GCTGTACTT

TATCCCAAG

ATCG=TTL

AAACCA.AAT'G

CAAGAATACA

ACCCTAGCCA

TAAAGGAAGA

CCACTCTTTT

AAACAGATAG

GTGC ErCAAT

CCATTCTCGC

TCCATGATAA

ATCATAATAA

CTGAAAATTT

GCTTACT- T

AAGATTCTGC

ATAATTAA

AATAAATAAA

CACCCCAACT

TAC'ITCTAGT

TAGTGCCAAG

ACCTACTCAC

GGAAAGAT

AAAAGTAACT

CAACATCGTT

A.AATGGTACC

TGACTTAGAA

F026-2 (SEQ ID NO:98) MKMSK VL'ITVLTATA ALVLLSACSS D)KKTDSSSSS KETANSSTEV VSGASISAKP EELEMALSDK G.NWIVAATDN NDVYRKA~y SQDDNI(KVTA EYEITVPKLI VS SENF'NIVH VNGN ITFDKQ EYKDSADLEK DGATVTGEVT VANN VTFDYEVIVA GTFHDKGKDS GTVKGDIEV< ANGFTLNGTK EFO26-3 (SEQ ID NO:99) AACACATAG TAGTTCTAGT ACCAAAGAAA CACCTAATTC AACTACACAA GTAGTCTCTC GTGC FrCAAT TAGTGCCAAG CCTGAAGAGC TCGAAATGGC GTTAACTC-AT AA.ACCAAATT CCATTCTCCC AGCTACTGAC AATCTCACTT TTCATAAAGA GGTAACAGTT GCTGGTAC'rr TCCATGATAA CGCAAAGAT 107 TABLE 1. Nucleotide and Anjino Acid Seqeuences of E. faecalis Genes.

TCCAACGATG

GCTGAATATG

CACGGGACTG

AAAGTTAATG

AAAGATGGTG

TCTATCGTAA

AAATCACGGT

TCAAAGGTGA

GCAATATTAC

CCACTGTTAC

ATTAGCACTT

TCCTAAGCTA

TATIG.AGGTG

TTGATAAA

TGGTGAACTC

TATTCCCAAG

ATCGTTTCTP

AAAGCAAATG

CAAGAATACA

ACCGTAGCCA

ATGATAATAA AAAAGTAACT CTCAAAATT CAACATCGTT GCTTTACT'Fr AAATGGTACC AAGATTCTGC TGAC'ITAGAA

ATAAT

EF026-4 (SEQ ID NO:100) TDSSSS S

KETANSSTEV

NOVYRKLALY

VNGNITFDKQ

VSGASISAKP

SQDDNKKVTA

EYKDSADLEK

EELEMAEJSDK

EYEITVPKLI

OGATVTGEVT

GNWIVA.ATDN

VSSENFNIVHi VANN- VTFDKEVTVA GTFHDKCXDS GTVKGDIEVK ANGFTLNGTI( EF027-1 (SEQ ID NO:1O1)

TTTGGTATGA

GCATGTGGAA

GTCGCATCTG

TCCGATATGA

GAGCTAGCTA

AAGTTACGAG

GCGTTTAGAA

TTTAAAAATG

GCAATCGATG

GTCTTGGTTG

GCCTATGGGA

GATGGCAATr

GTAAAATTGA

TTTGATAATG

GAGTCAAAAC

CGCCGTGAAA

GAAACTTTTG

GCTAATTTTG

TGCCATATAA

AACAGAAAAA

GTGGCGGTTC

GTGGTGAACT

TTGGTCAAGT

TGGCGAAAC

AAGCAAA.ATG

ACGTGGTCGA

GGCGTGCGGT

ACCAGACACT

GGACACCTTT

CTTCTGCAGA

CCGAAACTrG

ATGAAATTGA

GCGACTTAGA

.AGCGCATT

F1'ACCGGCAA

CAAAAGAAAT

CAAAAATCCA

TATTAAAGAA

GTGGTTAATC

GAAAACCACC

CTCGACATTA

AGTTGAAGGC

AGAGCCACAA

GACAAACGGG

TCCAGCATAC

GCGGGAAGGA

AGAACTAACA

TATGCCTAAA

TAIATTTTGTI'

GAAA ITGAAG

TGTI'CAAGTA

?I'ACACTGT

TGTACCTAAA

CGAACATGTT

TI'TAGGAGAT

GATACAGGG

CCCCAAGCTA

GGACTITGTTG

TCAAkACGAAC

GACAGCGCTC

TI'GTATCGAC

G ITAGTGAAG

GATCCAGT=A

GGTTCAAGTA

CAAGCCACGA

TTGGAAAATC

AATGAAGCCT

GGCAATGGCC

ALAGAATGATt

GTGAAAGAAA

TI'AGCAGATA

GCCATGGTGG

CGAAAAGC1T

GGCTCGACAG

AAGATI'TCCG

ACTGGAACAA

CACTGGGCTT

CAGCTACACA

ATTATACAGA

AAGATAAAAA

ACGGGTTACT

ALAGCAGGGGA

GCAGTAATCA

TGGAAGA.ATT

CAATTCCTTA

TT'GCCAAAGA

.CG?11'TAAT

ATTATGGGA

TTGGCACAGG

CF1'ATGCACT

GGTTTTAGCA

GAAAATTAAC

TGTCTATAGT

CGGAGATCCT

CTATACATTC

TTTTGTAGT

AATGGATATT

TGGTGTCAAA

F1TAGCCCAA

AAAAGGTACT

TTCAGGITGG

TAA.AGAACAC

AGCCAATCTT

TCAGTATAAA

G ITATTTAAG CCCCAATCAT T=ACAAGC GATTGACAAA C =TAAATGG NTTTGTACCA CAAAGAAAAT GGTGAITTAT

TT

a a' *a EF027-2 (SEQ ID NO:102) MXQKKWLI GLVALGLVLA ACGSGGISKTT SNEPATQKIN VASGGELSTL DSAHYTDVYS SDMIGQVVEG LYRQDKNGDP E LAMAKAEPQ VSEDGLVYTF KLREAKWqTWG DPVKAGDFVV AFRNVVDPAY GSSSSNQID I FKNGRAVREG QATMEEFGVK AIDDQTLELT LENP IPYL.AQ VLVGTPFMPK NEAFAKEKGT AYGTSADNFV GNGPFVISGW DGNSEIMKrLK KNDHYWDKEH VKLNEIDVQV VKEIGTGANL FDNGDLDYTV LADTYALQYK ESKQAJIFVPK AMVGYLSPNH RREITGNEHV RKAFLQAIDK ETFAXEILGD GSTALNGFVP ANFARIQIQV KISAXKM1VIY CHIILKKPKL TGTI EF027-3 (SEQ ID NO:103) AACGACC TCAAACGAAC CAGCTACACA GAAAATTAAC GTCGCATCTG GTGGTGAACT CTCGACATTA GACAGCGCTC TCCGATATGA TTGGTCAAGT AG ITGAAGGC TTGTATCGAC GAGCTAGCTA TGGCGAAAGC AGACCCACAA GTTAGTGA.AG AAGTTACGAG AAGCAAAATG GACAAACGGG GATCCAGTTA GCGT'ITAGAA ACGTGGTCGA TCCAGCATAC GGTTCAAGTA A~FrATACAGA

AAGATAAAAA

ACGGG~ITAGT

AAGCAGGGCA

GCAGTAATCA

TGTCTATAGT

CGGAGATCCT

CTATACATTC

TTTTGTAGTT

AATGGATATT

108 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

TTTAAAAATG

GCAATCGATC

GTCTTGGTTG

GCCTATGGGA

GATCGCAATT

GTAAAA=1GA

TTITGATAATG

GACTCAAAAC

CGCCGTGAAA

GALAAC'TI'TG

GCTAATTTTG

TGCCATATAA

GGCGTGCGGT

ACCAGACACT

GGACACCTI'

CTTCTGCAGA

CCGAAACTTG

ATCAAATTGA

GCGACTTAGA

AACGCATTT

TTACCGGCAA

CAAA.AGAAAT

CAAAAATCCA

TATTAAACAA

GCGGGAAGGA

AGAACTAACA

TATGCCTAAA

TAATTTTGTT

GAAATTGAAG

TCTTCAAGTA

TTACACTGTT

TGTACCTAAA

CGAACATGTT

'ITTAGGAGAT

GATACAGGTG

GCCCAAGCTA

CAAGCCACGA

TTGGAAAATC

AATGAAGCCT

GGCAATGGGC

AAGAATGATC

GTGAA.AGAA.A

TTAGCAGATA

GCCATGGTGG

CCAAAAGCTT

GGCTCGACAG

AGXI"TTCCG

TGGAAGAATT

CAATTCCTTA

TTGCCAAAGA

CGTTTGTAAT

ATTA'GGA

TTGCGCACAGG

CTTATGCACT

GTTATTTAAG

~TTTTACAAGC

CTTTAAATGG

CAAAGAAAAT

TGGTGTCAAA

TITAGCCCAA

AAAAGGTACT

'ITCAGGTTGG

TAAAGAACAC

AGCCA.ATCTT

TCAGTATAAA

CCCCAATCAT

GATTGACAAA

NTI'TGTACCA

GGTGA=TAT

EF027-4 (SEQ ID NO:104) TIT SNEPATQKIN VASGGELSTL DSAHYTDVYS SDMIGQVVEG LYRQDKNGDP ELAIAKAEPQ VSEDGLVYTF AFRNVVDPAY GSSSSNQMDI FKNCRAVREG QATMEEFGVK VLVGTPFMPK NEAFAKEKGT AYGTSADNFV GNGPFVISGW VKLNE I IVQV VKEIGTGANL FDNGDLDYTV LADTYALQYK RREITGNEHV RKAFLQAIDK ETFAXEILGD GSTALNGFVP

CHIILKKPKL

KLREAKWTNG

AIDDQTLELT

OGNSETWKLK

ESKQAHFVPK

ANFA.KIQIQV

DPVKAGDFVV

LENP IPYLAQ

KNDHYWDKEH

AMVGYLSPNH

KISAKKMVIY

0* EF028-1 (SEQ ID NO:105)

TAACAGAAGC

AAGAC'ITG'rr

AGAGC=IGC

TCTCAACAGA

GAAAAAGCAT

ACAACGGGCT

rTrGATACCT

ACCGATTCAG

ATTGCACTCG

GGGAAATCAA

GGCCACATA

CAAATCGACG

CGGAAAGATC

AAAAAGTCGT

CTACCTAAAA

GCTCTTCAAC

ATTGATTGGG

GAAGCGGCGT

CAACTGCAGA

AATACAACAA

ATAGTCAATG

TAGGGG'I-AC

AAAGCGGCGA

CAGTAAAAAA

ATCGCTA'TTT

AT'ITGGTCGG

C'I'ICCGCAGC

ATAATGACAA

CGGGTCT

ATG'I=CACG

GACAACACAA

GTGA'ITTAGT

TAAATGAGAA

TGA=IACCG

GGTTAGATAA

CCGGGCATAG

'ITGAAAAGGC

'PCATTCAACA

CTTAACACTT

TATGGGTAGA

CTTATTAACA

AAAACAAACA

TGTTA'TTT

CAAAGCCAAT

ACAGCAAGCC

GACAGCGATG

GTCCAAAACA

AGCAACATCT

CAAAAATATG

AGTCGATGTG

CAAAGAATTT

CCAAGACGAC

AACGGAAGAA

AAATGAAAA

TGT ITACTTG

TATGAAGGAG

'ITACAACAT

GAGGTTGCTG

ATGATTGGAG

CACTCAGACA

ACTTATCCAG

CCTGCCGGAG

GAAACAGTC

GAAATAACAC

GCAGAAATCG

TTAC'TGCGCG

TCCCAAGCGG

AAAAT=rAG

GTCCCTTCAT

GG'ITTC'ITTT

TTATTTATCA

GA.AACAAGGA

TAGCGGGTTG

A.AGCGAAGGC

ATGCCATGGG

AGCGTGTTCC

AAGATGAAGA

TGAAAACCTA

TCGAACGTGC

ATGCAACCCC

CCGATGACTA

GCGGCTCCGA

GTTATrCGTCA

GCTTGITTGC

TAGCTCATAT

TAATGG ITGA

TGAGCGAAAT

ATGGTGAACA

GAAATCAACT

AATGAAGAAA

TACAAATTTA

AACTGAATCT

GAATCCGTAT

CCAAACAGCT

AGAGAATGTC

TAATAATGCT

GAAAAAAGTG

TGCTGCATAT

'ITIGATGAT

ATTATTrCC

TGTCACAGAC

ACCAGGCGGG

CACAGAAGCG

AGGTACTCAA

GCAAGACTTC

TTGGTGGTTA

CAATGATATT GTTGGCGCGA CATCGATTTT GCCAAAAAAG GGGGGCTTrGT CTTTAG E-F028-2 (SEQ ID NO:106) MKKR ALLGVTLLTF TTLAGCTNLS EQKSGEKQTE VAFAXATESE KASVKNVIFM DTYLVGQQAT YPEDEEENVT DSASAATAMA KSTGLVATSE ITHATPAAYG AHNVSRKNMA KDRDLVKEFS QAGYCH-VTDK KSLNENQDDK LQRLDKNEKG FFLMVEGSQI DWAGHSMDIV

IGDGMGNPYT

AGVXTYNNAI

E-IADDYFDDQ

ILGLFAPGGL

GAMSEMQDFE

TGYRYFKANH

ALDNDI(SKTE

IDGQHICVDVL

PKMI DR"TIEEV

AA.FEKAIDFA

SDKRVPQTAF

TVLERAKKVG

LGGGSELFAR

PSLADMTEA-A

KKDGEHWLQ

TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

LQI IQQGACL EF028-3 (SEQ ID NO:107) ACAGA AAAGCGGCGA AAAACAAACA GAGGTTGCTG AAGCGAAGGC AACTGAATCT

GAAAAAGCAT

ACA.ACGGGCT

TTTGATACCT

ACCGATTCAG

ATTGCACTCG

GGGAAATCAA

GGCGCACATA

CAAATCGACG

CGGAAAGATC

AAAAAGTCGT

CTACCTAAAA

GCTCTTCAAC

ATTGATTGCG

GAAGCGGCGT

CAACTGCAGA

CAGTAAAAA.A

ATCGCTA=~

ATTTGGTCGG

CTTCCGCAGC

ATAATCACAA

CGGGTC 1TGT

ATG=TCACG

GACAACACAA

GTGATTTAGT

TAAATCAGAA

TGATTCGACCG

GGTTAGATAA

CCGGGCATAG

ETGAAA.AGGC

TCATTCAACA

TGTTArTT

CAAAGCCAAT

ACAGCAAGCC

GACACCGATIS

CTCCAAAACA

AGCAACATCT

CAAAAATATG

AGTCGATGWI

CAAAGAA'I=

CCAAGACGAC

AACGGAAGAA

AAATGAAAAA

CAATGATATT

CATCGATT'F

GGGGGCTTGT

ATGATTGGAG

CACTCACACA

ACTATCCAG

GCTGCCGGAG

GAAACAGTGC

GAAATAACAC

GCAGAAATCG

TTACTTCGCG

TCCCAAGCGG

AAAATTTTAG

GTCCCTTCAT

GGTTTCT

G'FrGGCGCGA

GCCAAAAAAG

CTT

ATGGCATGG

AGCGTCC

AAGATGAAGA

TGAAAACCTA

TCGAACGTC

ATGCAACCCC

CCGATGACTA

GCGGCTCCGA

G~ITATGGTCA

GCTTGTTTGC

TAGCTGATAT

TAATGGTGA

TGAGCGAAAT

ATGGTGAACA

GAATCCGTAT

CCAAACAGCT

AGAGAATGTC

TAATAATGCT

GAAA.AAAGTG

TGCTGCATAT

TTTTGATGAT

ATTATG C C

TCTCACAGAC

ACCAGGCGGG

GACAGAAGCG

AGGTAGTCAA

GCAAGACTTIC

TTGGTGGTTA

EF028-4 (SEQ ID NO:108) QKSGEKQTE VAEAKATESE KASVKNVIFM IGDGMGNPYT TGYRYFKANH SDKRVPQTAF DTYLVGQQAT YPEDEEENVT DSASAATANA AGVKTYNNAI ALDNDKSKTE TVLERAKKVG KSTGLVATSE ITHATPAAYG AHNVSRKNMA EIADDYFDDQ IDGQHKVDVL LGGGSELFAR KDRDLVKEFs QAGYGHVTDK KSLNENQDIJK ILGLFAPCGL PKMIIDRTEEV PSLADMTEAA LQRLDKNEKG FFLMVEGSQI DWAGHSNDIV GAMSEMQDFE AAFEKAIDFA KKDGEHWAWLQ LQI IQQGACL EF029-1 (SEQ ID NO:109)

TGAAGGAGGG

GCCACTTTT

GAAGTTACTG

GTTAGGCACG

CGGT'TAT

GGA'rrGGAAG

GAATATGATA

GATGGAACAT

AAAGAGCCTA

CAGTCACTCG

AGAAAATGAA

TATTATCAGG

AAAATGTTAC

TCATCGCGA

TAArI'GACAC

CTAGCAAACG

AGGGCGATAA

TACTACAAAA

CAACTAAGTA

GAATCTGGAG

AA.AGTTAATC

ATGCGCAAGT

TCAPLACTATI'

TACCACACTA

ACCCGAGACT

CACAAAAGAG

AACAGATCGT

AACGCTTGTA

TTTGGCAGAA

CATACCAGGT

GGTAAAAAGT

CTTGAACAAA

TCAAACGATC:

TTAAAAATTG

GTGAAACCGA

C7=TGTCTA

TACGGACGAG

AGTGAAGGAT

CTAGAGCAAG

TATCGTGACAC

GGCTGCTGCT

AAGCACAGGA

AACGTATACC

ACGGAAAACA

AAACAAACT

CCTI CAGA CGTTGGrGCTA

TAGCTCGTGT

CCCAAGAACA

AACGGGGG'TT

TACACCAGTA

TAGTGTAAAA

AGCTGATT

ACAAAAAGTT

TCAGCCGTTC

AATTACGTTT

CATATI'CGTA

TGCCTATGTA

GGCTAAAAAT

TAGTAAATAA

EF029-2 (SEQ ID NO:11O) MI(KLIG KKWLLLTAVA TFLLSGCASL EQKAQDSVKE VTENVTQTIS NDQRIPADFV RHVDGDTTVL KIDGKEQKVR FLLIDTPETV KPKTKVQPFG L.EASKRTKEL LSTASEITFE YDKGDKTDRY GRALGYIFVD CTLLQKTLVS ECrLARVAYV1( EPTTKYLAEL EQAQEQAKNE SLG IWSIPGY VIQRGFSK EF029-3 (SEQ ID NO:111) AAATGTTAC TCAAACTATT TCAAACGATC AACGTATACC AGCTGATTTT GTTAGCACC TGGATGGCGA TACCACACTA TrAAAAATTG ACGGAAAAGA ACAAAAAGTT 110 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

CGGTTTTTAT

GGATTGGAAG

GAATATCATA

GATGGAACAT

AAAGAGCCTA

GAGTCACTCG

TAATTCACAC

CTAGCAAACG

AGGGCGATAA

TACTACAAAA

CAACTAAGTA

GAATCTGGAG

ACCCGAGACT

CACAAAAGAG

AACAGATCGT

AACGCTTGTA

TTTIGGCAGAA

CATACCAGGT

GTGAAACCGA

CTTTTGTCTA

TACGGACGAG

AGTGAAGOAT

CTAGAGCAAG

TATGTGACAC

AAACAAAAGT

CTGCTTCAGA

CGTTGGGCTA

TAGCTCOTGT

CCCA.AGAACA

AACGGGGGTT

TCAGCCGTrc AATrTACGTTT~

CATATTCGTA

TGCCTATGTA

GGCTAAAAAT

TAGTAAA

EF029-4.(SEQ ID NO:112) NVTQTIS NDQRIPADFV RHVDGDTTVL KIDGXEQKVR FLLIDTPETV KPKTKVQPFC LEASKRTKEL YDKGDKTDRY GRALGY I VD GTLLQKTLVS EGLARVAYVK EPTTKYLAEL SLGIWSIPGY VTQRGFSK LSTASE ITFE

EQAQEQA(NE

EF030-1 (SEQ ID NO:113)

TGATTGACAC

GTTAGTACGG

ACTGAGAAAA

TCAATGGATT

GAAGGGTTAT

CCAGAAATTT

TCCA-ATGGTG

CCCAAAAACC

ATTACAAAAG

TTGGAGGTTA

TTTTTCCCAC

GAAATGATTG

TGGGATTTTIG

CATTTTGAAG

GATGTGGCTG

GCAATCGAAC

TCtATT'IrTG

TTAGTCGATA

TTTGTATATA

ACAGACGCCA

GTAGCCAITG

ATAGGGGGAA

TCTTGGTAGG

CCAGTGTCTC

CTAT'ITTTAC

ATCAATTGGA

CTG.AGGATGG

ATGCTG-TAAC

AAGCCA.ATTA

AGGAAAAAGC

C TTTAGAAAA

AAA.ACGAAGC

TATCTAATGG

TGCGTAATCC

TTCTTAAAGA

TCTTAACAGG

GGTCAAAAGT

CAAATCAGAA

ATATTTTAGC

ACCCAGAAAC

AAAAACCCAA

AACTTCTTTC

TAGTATGAAA

TTGTGGCACA

GAAAA.AAGTT

ACAAGATGAA

TGAAA.AAGAT

CAAACGATAT

AGCCAATGAT

CTTTTTCTTG

ACCAGAGGAA

GCCTGTACCA

ATTCGTGAAA

TCCGTTTTTA

CTACTATTAC

AACCAATACC

AGATTTTGCT

CTATTCCTTA

TGTCCCCAAA

AGATGGCTCA

GAATGAAGAT

AGAGTATTTA

AAGAGATGGT

AAGTTAAAAA TrATO-GGAT ACAGCAGANA CAAAAATAGA TTAAATTTAA TGGAGAACTC GCCAGTA ITA ACGCACAGTC CAACTAATAC CTGCTGCTGC ACCATTAAAC TAAGAGAAGA TCGTT?=G C'ITGCGTAA TTAGAAGGAA CGATTCTGAA TTGGGTGTCA AAGCGCTTGA TArI'TTACGT CG7TATTGGC GAAAAAGGAC AAGCCTATGC ATGAAAAATT GGGATCAGTC GATAAAGAAA AAGTAAAATC G ETATAATT Tr.TACGAATC AAACAAAATC GAGACAACCC CGTTTAAACC AAAAAAGAAA GCTTFIAGCTT ATGCTTTGGA AAAGAAATI'T ATGGGTACAT T'ITCGTCAAG AAGCAGGCGC GATAAAGCAA AACCAGAGCT GATAGTGACC GA

TATGTTATTT

CGAGAAAGCA

GGAAATCGGT

CAATGTCTTT

TAAAGAGATG

TGGCAAGTGG

ATTAGCGAAT

CGGAACAGCT

TGATTATACT

ATTTTCTCCA

CACTTCTAGT

AGCGATGTCG

AGAAACGATT

AGGTGAATTA

AGACTATGA-A

CGAAAAACCA

TAAAAAAAGT

TCCAGAAAAA

TCTTGTCAAA

AAACGGAGAT

EF030-2 (SEQ ID NO:114) MKK LKMMGIMLFV STVLJVGCGTT AXTKIDEKAT EKTSVSKKVL NLMrENSEIGS MDSIFTQDEA SINAQSNVFE CLYQLDEXDQ LIPAAAKEMP EISF.DGKRYT IKLREDGKWS NGDAV'TANDF VFAWRKLANP KNQANTYFFLL EGTILNGTAI TKEEKAPEEL GVKALDDYTL EVTLEKPVPY FTSLL.AFSPF FPQNEAFVKE KGQAYGTSSE MIVSNGPFLM KNWDQSAMSW DFVRNPYYYD KEKVKSETIH FEVLKETNTV YNLYESGELD VAVLTCDFAI( QNRDNPDYEA IERSKVYSLR LNQKRNEKPs IFANENVRKA LAYALDKKSL VDN~ILADGSK EIYGYIPEKF VYNPETNEDF RQE-AGALVKT DAKKAKEYLD KAKAELNGDV AIELLSRDGD SOR EF030-3 (SEQ ID NO:115)

GAGAAAGCA

ACTGAGAAAA CCAGTGTCTC GAAAAAAGTT ITAAATTTAA TCGAGAACTC GGAAATCGGT TCAATGGATT CTATTTTTAC ACAAGATGAA GCCAGTATTA ACGCACAGTC CAATG.TCTT GAAGGGITAT ATCAATTGGA TGAAAAAGAT CAACTAATAC CTGCTGCTGC TAAAGAGATG 1\

III

TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

CCACAAATT

TCCAATGTG

CCCAAAAACC

A'ITACAAAAG

TTGGAGGTTA

TTETTCCCAC

CAAATCATTG

TGGGATTTTC

CATI'TTGAAG

GATCTGGCTG

GCAATCGAAC

TCCATTTTG

TIAGTCGATA

TTTGTATATA

ACAGACGCCA

GTAGCCATTG

CTCAGGATGG

ATGCTGTAAC

AAGCCAATTA

AGGAAAAAGC

CTTAGAAAA

AAAACGAAGC

TATCTAATGG

TGCGTAATCC

TTCTTAAAGA

TCTTAACAGG

GGTCAAAAGT

CAAATGAGAA

ATATTrAGC

ACCCAGAAAC

AAAAAGCCAA

A.ACT'TCTTTC

CAAACGATAT

AGCCAATGAT

CTTTTCTTG

ACCAGAGGAA

GCCTCTACCA

ATTCGTGAAA

TCCGTT=TA

CTACTATTAC

AACCAATACC

AGArTTGCT

CTATTCCTTA

TGTCCGCAAA

AGATGGCTCA

CAATrAAGAT

AGAGTA!ITTA

AAGAGATGGT

ACCATTA.AAC

TTCcITT'

TTAGAAGGAA

TTGGGTGTCA

TAT'FrTACGT

GAAAAGGAC

ATGAAAAATT

GATAAAGAAA

GTTTATA.A~

AAACAAAATC

CGTTAA.ACC

TAACAGAAGA

CTGGCGTAA

CGATICTGAA

AAGCGCTTGA

CCTTATTGGC

AAGCCTATGG

GGGATCAGTC

AAGTAAAATC

TGTACGAATC

GAGACAACCC

AAJAAAAGAAA

TGGCAAGTG

ATTACGAAT

CGGAACAGCT

TGATTATACT

ATTTTCTCCA

CACTTCTAGT

AGCGATGTCG

AGAAACIGATT

AGGTGAATTA

AGACTATGAA

CGAAAAACCA

TAAAAAAAGT

TCCAGAAAAA

TCTTGTCAA-A

AAACGGAGAT

GCTTTAGCTrT ATGCTTTCGGA AAAGAAA=I ATGGGTACAT TTTCGTCAAr. AACCACCGCGC GATAAAGCAA AAGCAGAGCT EF030-4 (SEQ ID NO:2.16)

S

EKAT EKTSVSKKVL NLMENSEIGS MDSIFTQDE-A SINAQSNVFE GLYQLDEKDQ NGDAVTANDF VFAWRKLANP KNQANYFFLL EVTLEKPVP Y FTSLLAFSPF FPQNEAFVKE DFVRNPYYYD KEKVKSETIH FEVLXETNTV IERSKVYSLR LN'QKRNEKPS IFANENVRXA VYNPETNvEDF RQEAGALVKT DAXKAXEYLD LIPAAAKEMP EISEDGKRYT EGTILNGTAI TKEEKAPEEL KGQAYGTSSE MIVSNGPFLM YNLYESGELD VAVLTGDFAK LAYALDKKSL VDNILADGSK KAKAELNGDV AIELLSROG

IKLREDGKWS

GVKALDDYTL

KNWDQSAMSW

QNRDNPDYEA

EIYGYIPEKF

EF031-1 (SEQ ID NO:117)

TGAGAAATTA

AAACGCGTAA

AGCGAAGCAA

TTCGGATTAA

GAAGCGAAAG

AATCCCAATG

AAAGATCGGT

CCGGGAGCAA

GGGATTGTTT

TCAGCTGATT

ATGTTATACG

GCT'TGAAG

GACTTAGCNA

GTTGATATTA

GTTATTTTAG

TTTAGGGAC

CTACGAAAAG

GTGAAGATAT

TTACCTTAGA

CGGGAA 1MA

TATTM'AAAA

A.AGAGGTI'T

ACAACAAAGA

TGAAAGGTAA

TT'GCTAGTGA

CGATGAAAGA

ATATGTTCCA

TTCAAGGCGC

AAAAATAAAA

ATTAGTCGCT

CCAGAGCAAA

TGTCAAAAAA

AGTAGGCAAT

TGTCAT1'GAA

AATTACAGAA

TGAAAGTGGT

AAAATTAGGC

AATTTCTGTT

ACATGCTGGT

ATTAAAACCA

ATCTCGTCAA

ACAGAAAACG

ACCATTTGG

GCAACGTTAT

GGTGGAAGTA

GACATI'ATCG

AGCGCAGACC

TTAGCACAAG

AAAOAAGTAC

GCTGGCGTAC

ACGGAAGMTT

TAATGACTGC

ATGCT~AGT

CTCCATMA

GCTTACGAA

CAAATGCAC

CTA-ATITAAC

CAATCGCTGT

AAAAATGAAA

TTGTGGAAAC

CG=TCAACT

AAAAGAGAAT

ATTAAAkAAAT

ACAAGGTGGA

TCAGTTAACG

AAACAGTATC

TGACTTATGG

AGGTCCTTrA

CGGGAAGGCC

AAAATCGTCA

TGAT TTCCG AAAGAAATTA AAAACTGGGA CCAGACCTTG CCACGACGCC CAAGATATTA CAAAAGATAA AACG'ITTTA AAACGTATTWC ATTGAACCAC CTCTGCTrC

TGA

EFOO31-2 (SEQ ID NO:118) MKK RVILGTLVAA TLLMTACGNS EATTKSESKG GSNALVVSTF CLSEDIVKKD I LAPFEKENE AKVTLEVGNS ADRPTKL.KNN PNAGIDVIEL AQANAAQGGK DGLFEKITEK EVPNLSQLTP GAKEVFESGA GVPIAVNSIG IVYNKEKLGK EIKNWDDLWS ADLKGKI SVP DVATTAGPLM LYVASEHAGQ DITKIDNGKAA FEAWCEU(PN VVKTYSKSSD LANMFQSGEI EAAVVADFAV DIIQGAQKT EF031-3 (SEQ ID NO:119) 112 TABLE 1. Nucleotide and Amino Acid Seqeuences of Efaecalis Genes.

AA CTACGAAAAG CGAGAGCAAA GGTGGAAGTA ATGCT=AGT CGTTTCAACT

TTCGGATTAA

GAAGCGAAAG

AATCCCAATG

AAACATGGGT

CCGGGAGCAA

GGGATTGTT

TCAGCTGATT

ATGr1'ATACG GCTTT1TGAAG

GACTTAGCNA

GTTGATAFrA

GTGAAGATAT

TTACCTTAGA

CGGGAATTGA

TATTTGAAAA

AAGAGCTTTT

ACAACAAAGA

TGAAAGGTAA

TGCTAGTGA

CGATGAAAGA

ATATGTTCCA

TTCAACGCC

TOTCAAAAAA

AGTAGGCAAT

TGTCATTGAA

AATTACAGAA

TGAAAGTGGT

AAAATTAGGC

AATTTCTGTT

ACATGCTGGT

ATTAAAACCA

ATCTGGTGAA

ACAGAAAA

GACATTATCC CTCCATTTGA AGCGCAGACC GCTTLTACCAA TTAGCACA-AG CAAATGCAGC AAAGAAGTAC CTAA=tA.AG GCTGGCGTAC CAATCGCTGT AAAGAAATTA AAAACTGGGA CCAGACGTTG CCACGACGGC CAACATATTA CAAAAGATAA AACGTTGTTA AA.ACGTATTC ATGAAGCAG CTGTGGITGC

AAAAGAGAAT

ATTAAAA.AAT

ACA.AGGTGGA

TCAGTTAACG

AAACAGTATC

TGACTTATGG

AGGTCCTTA

CGGGAAGGCC

AAAATCGTCA

TGATTTTGCG

EF031-4 (SEQ ID NO:120) TTKSESXC GSNAL.VVSTF GLSEDIVKKD IIAPFEKENE AKVTLEVGNS ADRFTKLKNN' PNAGIDVIEL DGLFEKITEK EVPNLSQLTP GAXEVFESGA GVPIAVNSIC IVYNXEKLGK ADLKGKISVP DVATTAGPLM LYVASEHAGQ DITKDNGKAA FEAMKELKPN LANNFQSGEI EAAVVADFAV DIIQGAQK

AQANAAQGGK

EIKNWDDLWS

VVKTYSKSSD

S

5*5**

S

C

EF032-1 (SEQ ID NO:121)

TGAATAAATT

GTTTGTGGTA

AAGTCAACCA

TCAGGGGAAT

CAACTAGATG

AACCATGACT

CTCACATTCG

CAAGAATATA

TCTGATGTT

GAAGTAACTA

AATACTAATC

GATACTTTAG

TCAATTATCG

A ETTAGGAGG

TTTCACTACT

GTCAATCTAG

ATTCAGTT'-G

ATAAATCGAG

TATACGGAGT

AAACTGCCGA

TGAAAA-ATCC

CTAAAAGTAG

CTGAAGCGAA

ACATTACTAA

CTAAGCATCA

GCGTCATCCC

A.ATTATGATG

TACTGCTTGT

CAGCACAGTT

AAAAGATATT

CATAGTTCTT

GGGAAACAAG

CAAAGATTr

AGTATCNACT

TAGCCAAGAT

GTCTGATGTA

GCTAGCAGAT

ATTTAATGAT

AACCATGGAC

AAAAA.ATTAA

NCGGGAAATA

AAACAACCGA

GATCCTGGAG

ATTACCGTCA

AAAAAAGTAT

GTTGTTAGAT

ACTGAAACTA

AATAAACAAT

GCTACTAATA

GAGCCAACCT

ATGTATCCTI'

GCAAAAAGAT

TTAG=IAGG

ATGATAATAA

ATTCAAAAGA

ATTACTATGC

AATCAGGCCG

CTCTTAAAAA

TTITAAATGA

GCAAACANAA

CTGATGTATC

CTTTACCGAG

TAGAACAACA

ATAA-AGGAAG

GGTAA

ATTGG'TTGT

AGATACTGAA

CTTTGTTGCG

TGTATITAACT

ACAAAATAGT

GGGAGATACT

AAAAGATTTT

AACACTAAAC

TGAAAAAAA.A

CGAAGATAAA

AACCGTC'TTA

CAAAATGCAT

EF032-2 (SEQ ID NO:122) MK KLISLGLAVCV CGISLLTACX GNNDNKDTEK STSQSSSTVK QPNSKDFVAS GEYSVGKDID PGDYYAVLTQ LDDKSSIVLI TVKSGGENSN HDLYGVGNKK KVSLKKGDTL TFETADKDFV VRFLNEXDFQ EYMKNPVSST ETSKXKTVNS DVSKSSSQDN KQSDVSEKKE VSTEAKSDVA TNTLPSEDKN TNOITKLADE PTL.EQQTVLD TLAKHQFNDM YPYKGSKMHS IIGVIPTMDA KRW EF032-3 (SEQ ID NO:123) TA ATGATAATAA AGATACTGAA AAGTCAACCA GTCAATCTAG CAGCACAGTT AAACAACCGA ATTCAAAAGA CTTrGICCG TCAGGGGAAT ATTCAGTTGG AAAAGATATT GATCCTGGAG ATTACTATGC TGTATrrAACT 1.13 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecais Genes.

CAACTAGATC

AACCATGACT

CTCACATTCG

CAAGAATATA

TCTGATGT

GA.AGTAAGTA

AATACTA.ATG

GATACTTTAG

TCAATTATCG

ATAAATCGAG

TATACGGACT

AAACTGCCGA

TGAAAAATCC

CTAAAAGTAC

CTGAAGCGAA

ACATTACTAA

CTAAGCATCA

GCGTCATCCC

CAkTAGTTCTT

GGGAAACAAG

CAAAAT

AGTATCNAGT

TAGCCAAGAT

GTCTGATGTA

GCTAGCAGAT

ATTTAATGAT

AACCATGGAC

ASACCGTCA

AAAAAAGTAT

GTTAGAT

ACTG;AAACTA

AATAAACAAT

GCTACTAATA

GAGCCAACCT

ATGTATCCTI'

GCAAAAAGAT

AATCAGGCGG

CTCTTAAAAA

TTTITAAATGA

GCA.AACANAA

CTGATGTATC

CTTTACCGAG

TAGAACAACA

ATAAAGGA.AG

GG

AGAAAATAGT

GGGAGATACT

AAAAGATT

AACAGTAAAC

TGAAAAAAAA

CGAAGATAAA

AACCGTCTTA

CAAAATGCAT

EF032-4 (SEQ 10 NO:124) NDNKDTEK STSQSSSTVK QPNSKDFVAS GEYSVGKDID PGDYYAVLTQ LODKSSIVLI TFETADKDFV VRFLNEI(DFQ EYMKNPVSST 'JSTEAKSDVA TNTLPSEDKN TNDITKLADE IIGVIPTMDA KRW TVKSGGEFNSN HDLYGVGNKK XVSLYKDTL ETSKXKTVNS DVSKSSSQDN KQSDVSEKKE PTLEQQTVLD TLA'4QFNDM YPYKGSKMS EF033-1 (SEQ ID NO:125)

TGACTGCTTT

CAAAGAGGT

AAAATGAAAA

GCAGGATGTG

TTACCAACCA

ATTTCCCTAG

ATCGCAGTTG

GATATGATG

AATGACATCA

ACAGTCGTTA

ATCCCTGATA

GAAATCGACG

TTT~TCTATTG

TCkITTCAGA

AAITTTACTTI

GTAAACAGGA

AAGACCGTAG

TGCCATCAAC

ATACTCAAAG

CTCTCGATGC

ATTAGCTAG

ATATCCCCAC

GC PTATCTGA

AGTAG

GAGAAAAAAG

AAA 'rTCCC

AACAATGATG

AAAGAAAGCA

CGGCAAAGAA

AACAGA.AGTG

TAGTACAATG

CGAAAAATTG

CTCAGAAAGT

TAGTACAAGC

ACATGAAAAA

TGGT=ITTTT

CA.AAATAAAA

AC EAGGIT

ACTACCTCTT

ATTACTTTAC

ATIGAAGACT

ATGACTGATT

ATTGCCTTGA

GT LTGGAAGC

ATCAAAGCAA

GGACAA.AAGT

GTATTG7Wfl

TAGACGAATC

TAGTACCAAC

CTGAAAAAAC

CCAAAGAAGC

TAGGTAAAAC

TAAAAAAATT

AACCACAAAT

AAGTrGCAAGA

TCAAAGAAGA

TAATCAAAAC

GACGTTGAGA

CGAGGATCAA

ACTTGGC TTA

AGAAGTAACG

AACCAAAATT

CGACCAA'ITA

ACCACAAATG

TGTTTATGTG

TGCTGGA-AT

CGTCCAATTC

AATGGATCAA

EF033-2 (SEQ ID NO:126) S S MKKFTLTMMT LGLVATLGLA GCGKQEKXAT TSSEKTEVTL AVDTQSSTMM TDLKKLPQMD VVNIPTSTSI KAIKEDVQFI PTKDRSGKEI TLPKEATKII SLVPST.PEVI EDLGKTDQLI MAVDAEKLI ALKPQIVY-VN DINLASSESV WKQVEDAGIT ADSL.SEHEKG QKLIKTMDQE IDE EF033-3 (SEQ ID NO:127) CTCTT CTGAA.AAAAC AGAAGTAACG

TTACCAACCA

ATTTCCCTAG

ATCGCAGTG

GATAT'GATGG

AATGACATCA

ACAGTCGTTA

ATCGCTGATA

AAGACCGTAG

TGCCATCAAC

ATACTCAAAG

CTGTCGATC

ATTTAGCTAG

ATATCCCCAC

GCTTATCTGA

CGGCAAAGAA

AACAGAAGTG

TAGTACAATC

CGAAAAATTG

CTCAGAAAGT

TAGTACAAGC

ACATGAAA.AA

ATI'ACTTTAC

AMrAAGAC'I

ATGACTGATT

ATTGCCTTGA

GTTTGGAAGC

ATCAAAGCAA

GGACAA.AAGT

CCAAAGA.AGC

TAGGTAAAAC

TAAAAAAAT'

AACCACAAAT

A-AGTGGAAGA

TCAAAGAAGA

TAATCAAAAC

AACCAAAAT

CGACCAATTA

ACCACAAATG

TG'ITIATGTG

TGCTGGAATT

CGTCCAATTC

AATCCATCAA

GAA.ATCGACG AGTAG 114 TABLE 1. Nucleotide and Amnino Acid Seqeuences of E. faecalis Genes.

EF033-4 (SEQ ID NO:128) SSEKTEVTL PTKDRSGKEI TLPKE-ATK<II SLVPSTTEVI EDLGKTDQ:LI AVDTQSST2d TDLKKLPQMD MIMAVDAEKLI AL.KPQIVY-VN DINLASSE-SV WKQVEDAGIT VVNIPTSTSI KAIKEDVQFI ADSLSEHEKG QKLIKTMDQE IDE EF034-1 (SEQ ID NO:129)

TAGGAGGGAG

TTGGTAGG'T

CCTTTAATAC

TTCAACAAGG

GACACAAAAA

AATAAAGAGA

AAAAAAGTTG

TAATCATCAA

GTAGTAATAA

TCACCACGAT

ATAAAACCAT

AAACAAGTAG

GCTATGAAAT

ATCAAGGTAA

AAAAATCGGG

CAAAAAAGAA

TATGAAAAA

GACGCTTGAA

AACAGA-AAAA

TATAGGTCAA

ACGTATATCT

TATI=rAG'Pr

AACGGCAATC

GAAGAAGACC

AAAGAATATI'

AAGGTATATA

'ITGGACAAAA

GATGCAGAAG

GTATTATTTT

'-TTTGAATGC

TAACGAAAGG

TAGTTAATCC

AAAATATTAA

AAACGAAAAA

GTAATGTCTA

T'ICATGTI'T

CAGTTCGTI'

TTCAATTTTT

CAATAATGALA

AATACAAGAA

AATAGAGTTT

TGGTGAT=rI GGTGGTAAAT AG EF034-2 (SEQ ID NO:13O) MrKKIGY FSCIIFFMFL VGCSNNKKEN GNLLNASSFP LILTTIIEKE EDLTKGSIFF NKDKTMTLEK EYLVNPNNED TKKTSRTEKX VYKNIKIQEN KESYEIIGQL DKKTKKIEFK KVDEGKRISD AEGNVYGDFG GK EF034-3 (SEQ ID NO:131) AGAA AACGGCAATC TTTIGAATGC CAGTTCGTTT CCTTTAATAC TCACCACGAT TATTGAAAAA GAAGAAGACC TTCAACAAGG ATAAAACCAT GACGCFI'GAA AAAGAATATT GACACAAAAA AAACAAGTAG AACAGA-AAAA kAGGTATATA AATAAAGAGA GCTATGAAAT TATAGGTCAA TTGGACAAAA AAAAAAGTTG ATGAAGGTAA ACGTATATCT GATGCAGAAG GGTGGTAAAT AG TAACGAkAAGG

TAGTTAATCC

AAAATAI'1AA

AAACGAAAAA

GTAATGTGTA

'ITCAATT1TTT

CAATAATGAA

AATACA.AGAA

AATAGAGTT

TIGTGATTTT

0 EF034-4 (SEQ ID NO:132) KEN GNLLNASSFP LILTTIIEKE EDLTKGSIFF NKDKTMTLEI( EYLVNPNNED TKKTSRTEKK VYKNIKIQEN KESYEIIGQL DKKTKXIEFK KVDEGKRISD AEGNVYGDFG GK EF035-1 (SEQ ID NO:133)

TAAACGAGAG

TTATTCACAA

ACAATTGATC

GAAAATTCCA

AA.AATCGATA

GTGAGTTTAT GAAAACAAAA ATCGGAAAAA G'TrTCCT=r ACTGAGTGGT TGTACCTCGG GACAGAAAGA AAAAGTCGAT AAA.ACGGTCG TGGAAAGTTA CGACGAAAAA GTTGACCGTT CTACTGAGTA A

CAGTTATCTI'

CTGGCGAAGA

ATAAGCACAA

CTTTAGATAG

GTCAGCATTT

GATGGAAAA-A

ACATAAAAAT

TCAAGAAGAC

Er035-2 (SEQ ID NQ:134) M~KTKI GKTVILSAFL FTSFLLLSGC TSAGEEET IDRQKEKVDK TLVDKQKHKNE NSMESYDEKV DRSLDSQEDK ID~rE 115 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

EF035-3 (SEQ ID NO:135)

GATGGAAAAA

ACAATTGATC GACAGAAAGA AAAAGTCGAT AAAACOCTCG ATAAGCACAA ACATAAAAAT GAAAA LTCCA TGGAAAGTTA CGACGAAAAA GTTGACCGTT CTTTACATAG TCAAGAAGAC AAA.ATCGATA CTACTGAG EF035-4 (SEQ 10 NO:136) MEKT IDRQKEKVDK TSJDKQKHKNE NSMESYDEKV DRSLDSQEDK IDTTE EF036-1 (SEQ ID NO:137) *9

TAATTTTCAA

AAT'GAATCGA

ACAGGATGTG

TTACAACCAT

ATTAATGTCC

GACATTGGTA

ATTGATCATA

AAAGATATCT

GAACTTGGCG

CGTGCGACTT

GATTCCAGCG

GCATTTTCAT

GATGAAAATG

AAAAATCCAA

GAACGTGTGA

TGGCAAGGAA

GTCCTACATA

TGAAAAAAAG

CAAAATGGAT

TAGTAGAGAC

AAGGTGGCGG

AI'CTGAI'T

AAGTTGCTGT

CAATGGAAAA

GGAAAGACCA

TTGAAAAGTG

GCATGGTTCG

ATGTTACTGA

TAATGAACAA

GTGATTTAAC

TTGGTCAA.TT

ATGTCATTAA

TAATGGTAAA

ATTGCTATTA

TGATCGTGGT

AGCGAGTGAG

AAGCGGAACA

ATTTGCAGAA

CGTTGGGATT

TTTAA.AGAAA

AAAAATTGTT

GGTCTTGGGA

TTCCATTGI'

TGAAGTAGCT

TAAATGGATT

CAAAGAGTT

AGGGTATATT

ATAA

ATAGAATGGA

TTTATTrG=

GAATCCATCA

GAATATCAAA

GGTCTGAGTC

GAGAAAAAGG

ACACCAATCG

T'rGA.AA'TAA

TGGCAAGTAT

CAGCGGTAGG

GCCAAAATCC

AAGTCCAATC

GCATCAkAGC

TTAACAAAAA

ATCITITAG GTGAAGTAAC ATTT'GAATA GAGCGGCCGG GATAAAACAG CCATTCGTGC TCTGATACAC CAGGAGCGAT ACGTTAAGTA TTGATGGTGT ATIWGGTCTT ATGA-ACACAT TTAGACTTTA TGTTGTCAGA CCTGTI'CGA AAATGGAAAT

TTGGAGGAAT

ACTTACTT

CTCATCAGCT

GGGAAGATTT

TGGCGCGGTA

GGAAGACTTA

TGTCGGTGTC

AAACTGGAAA

TAGTGGTACG

GCAAGAACAA

TAGTTATACC

TCAGCCA.ACA

GTACACTCGT

TGATATCCAA

TGAACGGGAT

EF-36-2 (SEQ ID NO:138) MKKRLLLF IGLASILTLT GCAXWIDRGE SITAVGSSAL QPLjVETASEE YQSQNPGRFI NVQGGGSGTC LSQVQSGAVD IGNSDLFAEE DHKVAVVGIT P IVNKNVGVK DI SMENLKKI FLGEVTNWKE LGGKDQKIVI ATFEKWVLGD KTAIRAQEQD SSGMVRSIVS DTPGAISYTA FSYVTDEVAT ENVMNNKWII WSYEHMYTRK NPSDLTKEFL DFMLSDDIQE RVIGQLGYIP

KKGIKAEDLI

LNRAAGSGTR

LS IDGVQPTD

VSKMEIERDW

EF036-3 (SEQ ID NO:139) GAT TGATCGTGGT GA.ATCCATCA CAGCGGTAGG CTCATCAGCT

TTACAACCAT

ATTAATGTCC

GACATTGGTA

ATrTCATCATA

AAAGATATCT

GAACTTCCCG

CGTGCGACIT

GATTCCAGCG

TAGTAGAGAC

AAGGTGGCGG

ATTCTGATTT

AAGTTGCTCT

CAATGGAAAA

GGAAAGACCA

=ITAAAAGTG

GCATGGTTCG

AGCGAGTGAG

AAGCGGAACA

ATTTGCAGAA

CGTTGGCATT

TTTAAAGAAA

AAAAATTGTT

GGTCWIGGGA

TTCCATTGTT

GAATATMAAA GCCAAAATCC GGTCTGAGTc AACTCCAATC GAGAAAAAGG GCATCAAAGC ACACCAATCC TTAACAAAAA ATCTTTAG CTGA-AGTAAC ATTTGAATA GAGCGGCCGG GATA.AAACAG CCATITCGTGC TCTCATACAC CAGGAGCGAT CGOAACAT1T

TGCGCGGTA

GGAAGACTTA

TGTCGGTGTC

AA.ACTGGAAA

TAGTGGTACG

GCAAGAACAA

TAGTTATACC

116 TABLE 1. Nucleotide and Amrino Acid Seqeuences of E. tieca lis Genes.

GCATTT'rCAT

CATGAAAATG

AAAA.ATCCAA

GAACGTGTGA

TGGCAAGGAA

ATGTTACTGA TGAAGTAGCT TAATGAACAA TAAATGGATT GT'GATTI'AAC CAAAGAGTI TTGGTCAAI' AGGGTATATT ATGTCATTAA A

ACGTTAAGTA

ATTTGGTC'TT

TTAGACTTTA

CCTGTTT'CGA

TTGATGGTGT

ATGA-ACACAT

TGTTGTCAGA

AAATGGAAAT.

TCAGCCA.ACA

GTACACTCGT

TGATATCCAA

TGAACGGGAT

EF036-4 (SEQ ID NO:140) IDRGE SITAVGSSAL QPLVETASEE YQSQNPGRFI NVQGGGSGTG DHKVAVVGIT PIVNXNVGVK DISMENLKKI ATFEKWVLGD KTAIRAQEQD SSGMVRSIVS ENVMNNKWI I WSYEHMYTRK NPSDLTKEFL

QGNVIK

LSQVQSGAVD

FLGEVTNWKE

DTPGAI SYTA

DFMLSDDIQE

IGNSIDLFAEE

LGGKEDQKIVI

FSYVTDEVAT

RVIGQLCYI P

KKGIKAEDLI

LNRAAGSGTR

LS IDGVQPTD

VSKMEIERDW

EF037-1 (SEQ ID NO:141) a.

a.

a a a a. a

TGAGTGTATG

TTI'TTTGGAG

GCTGCTCTTG

AGCAAAGAAA

CCTGAAGAGC,

AATG-TCACTT

TCCAACGATG

GCTGAATATG

CACGGGACTG

AAAGT'AATG

AAAGATGGTG

ATTACTCAT

GGATT ITCAT TGTTGCTrAG

CAGCTAATTC

TCGAAATGGC

TT1GATAAAGA

TCTATCGTAA

AAATCACGGT

TCAAAGGTGA

GCAATAITAC

CCACTGTTAC

TCCCTTTGAA

GAAAATGTCT

TGCTTGTTCA

AAGTACAGAA

GTTAAGTGAT

GGTAACAGT'

ATTAGCACTT

TCCTAAGCTA

TATTGAGGTG

TTTTGATAAA

TGGTGAAGTC

TCAGTTATGA

AAAGTACTCA

TCTGATAAAA

GTAGTCTCTG

AAAGGAAATr

GCTGGTACT

TATTCCCA.AG

ATCGTTTCTr

AAAGCAAATG

CAAGAATACA

ACCGTAGCCA

TAAAGGAAGA

CCACTGTTTT

AAACAGATAG

GTDCTTCAAT

GGATTGTCGC

TCCATGATAA

ATGATAATAA

CT0AAAA=T

GCTTTACI''

AAGATI'CTGC

ATAA

AATAAATAAA

GACGGCAACT

TAGTTCTAGT

TAGTGCCAAG

AGCTACTGAC

GGGGAAAGAT

AAAAGTAACT

CAACATCGTT

AAATGGTACC

TGACTTAGAA

EF037-2 (SEQ ID NO:142) MKMSK VLITVLTATA ALVLLSACSS DKKTDSSSSS KETANSSTEV VSGASISAKP EE.LEMALSDK GNWIVAATDN VTFDKEVTVA GTFHDKGKDS NDVYRKLALY SQDDNKKVTA EYEITVPKL I VSSENFNIVH GTVKGDIEVK ANGFTLNGTK VNGNITFDKQ EYKDSADLEI( DGATVTGEVT VANN EF037-3 (SEQ ID NO:143) AACAGATAG TAGTTCTAGT AGCAAkAGAAA CAGCTAATI'C CCTGAAGAGC TCGAAATGGC AATGTCAC IT TTATAAAGA TCCAACGATG TCTATCGTAA GCTGAATATG AAATCACGGT CACCGACTO TCAAAGGTGA AAAGTTAATG GCAATATTAC AAAGATGGTG CCACTGTTAC

AAGTACAGAA

GTTAAGTGAT

GGTAACAGTT

ATTAGCACTT

TCCTAAGCTA

TA'ITGAGGTG

TTIATAAA

TGGTGAAGTC

GTAGTCTCTG

AAAGGAAATT

GCTGGTACTT

TATTCCCA.AG

ATCGTITCTT

AAAGCAAATG

CAAGAATACA

ACCGTAGCCA

GTGCTTCAAT

GGATTGTCGC

TCCATGATAA

ATGATAATAA

CTGAAAATTT

GCTTTACTTT

AACA~CTGC

TAGTGCCAAG

AGCTACTGAC

GGGGAAAGAT

AAAAGTAACT

CAACATCGTT

AAATGGTACC

TGACTTAGAA

EF037-4 (SEQ ID NO:144)

TDSSSSS

KETANSSTEV VSGASISAXP EELEMALSDK GNWIVAATDN VTFDKEVIVA GTFHDKGKDS NDVYRKLALY SQDDNKKVTA EYEITVPKLI VSSENFNIVH G IVKGDIEVK ANGFTLNGTK 117 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

VNGNITFDKQ EYKDSADLEK DGATVTGEVT VANN EF038-1 (SEQ ID NO:145)

TAATGGCCAT

AACAAGGATC

GTCGGC2~rGT

CTCGTAAAT

GAAkACT7LT

AATAATAAAA

GAATATAAAG

TTCCTCTACT

ATAAAAAAGG

TATTGTTGTC

GCAACTAGA

CGAGGTATAA

AACTCACTTT

ACGGTAAATT

AATAAAGAGG

AGAAGTGAGC

AGGT'GTGGA

AAATAAAAGT

TTCAAAAATTr

GGATATAAA.A

AAAAGGTGA-A

ATGAAGCTAC

ATGAAAAAAG

ACAGATATGA

CCAACTACTA

AGTrGATAGTG

AATAAAGAAC

ATrGAGGCG

TCAA-ATGGCG

TACTACCTTT

AAAAGATATT

CTTACACTTT

GAACGTACTC

AATTAATA.AT

AGAALGGACTC

TTGGCAATGG

TAT1'GCCTTA

GACTGCCGA*T

TITTGATCAT

TTACGATGAA

GGAAAATGTT

TGATAAAAAA

TNGAATAAGA GTGTCTTTG A EF038-2 (SEQ ID NO:146) M KLLKWRWQWN KDHKXGEVSM KKVLPFIALV GLLLLSGCGT DI~fl(KILTADG GKWELENXSP 'PTTYTFFDDE TFSRYNSKIS DSGTYSYDEN NKKLTLDIKN KEQLIMENVE YKDGKLKGEI GGEKDSDKKX NKRCL EF038-3 (SEQ ID NO:147) TTGTGGA ACAGATATGA AAAAGATAT GACTGCCAT GGTGGTAAAT GGGAACTAGA AAATAAAAGT CCAACTACTA GAAACIT'N CGAGGTATAA TTCAAAAATT AGTGATAGTG AATAATAAAA AACTCACTT GGATATAAAA AATAAAGAAC GA.ATATAAAG ACGGTAAATT AAAAGGTGAA ATTGGAGGCG TNGAATAAGA GGTCTTT=G A

CTTACACTTT

GAACGTACTC

AATI'AATA-AT

AGAAGGACTC

TTTTGATGAT

TTACGATGAA

GGAAAATGTT

TGATAAAAAA

EF038-4 (SEQ ID NO:148) CGT DMKKILTADC GKWELENKSP TTTYTFFDDE TFSRYNSKIS IJSGTYSYDEN N'KKLTLDIKN KEQLIME-NVE YKDGKLKGEI GGEKDSDKKX NKRCL EF039-1 (SEQ ID NO:149)

TAAATATATC

GCGC ITACCT

TCTGCAACAG

GCAGCTAAAG

CAAGGCACAA

A=TG'ITAG

GGTGAAACCC

TTAATTTCGG

AAATTTACCT

GAACATATCC

ACAGAAACTG

TGGAATGCCA

TI'TATI'AGTT

ACGGGTAAAA

GCTCACGTTT

GATAAACGTT

AAAAAGAAAA

TATTAACCTT

ATAAATCAAG

AGCAATCAAA

AAGTTI'ACCA

CAAAATATGA

GTGGCGAGA

ATACACAAAA

ATAAGCGAAT

CTATTCTC.A

GCAAGATTGT

CGAAAGTFr'r TACC.GAAA1T

CACGTGGAGA

CAATTGGTGA

TTACGTATAC

AAGGGGATTA

TAGTACGTTG

TGCAGCTAGC

AGGACAAGAA

CAAAAATNAT

TGGTGAAACA

AGGCACATI'C

CTATCAAGCG

GGGTAAAGAT

CGAGAAA LTA

TACCAATGAA

AGATGAAGAC

TGATAATAAA

TTTTGGTTAC

CAATAAATAT

ACGAATGCGT

CCAACCATGA

TTGATTGCAG

TCAAGCACT13

TAACAGAAA

AATAATTTAA

GGTFI=ATG

TTTGTGACAG

GTGGTCGAT

AAAGACGGGA

ACCT'ITACGA

CCTGGGGATG

GGTAACGATG

ACAAGTAAAT

TI'CCAAGTA-A

GGAGCTGCAT

AAAGACKACA

AAAAGAAAA-A

GCTGTGCTGG

CAGTCTCTAG

TTTTrATCCAG

CAGCAGAAA

AATTTTTCGA

ACGATGGCGA

TAACGGAAGT

AAGATGTAGA

ACGGCCGTAA

ACATTTAGG

TTACTGAAGC

ATGA.ATICTT

TTATAAT;A

TAGAATTAAC

ATGGCAAAGA

AGTTITTAGT

CGGAGCCAAC

TTCAGCAGAA

TACTGATTG

TGCTAATTT

CAAAGAAACA

AAAGCGTATC

GACGAAAGAT

AGTCTITGTA

AGATAGAA

GGCcACATTA

AAATAAAATG

TGATTTAGA-A

CAAAATCCGT

AGAA'ITAAAT

AATTAAAGTC

TABLE 1. Nucleotide and Amino Acid Seqcuences of E.faeca lis Genes.

TTTGTAGAAC ATGAACCATA TGAAGGAGAC TTTACGCCAG ACTTCACGTT CTAA EF039-2 (SEQ ID M~KKKVFSA LTLLTFSTLL IACCAGGANS ATDKSSA.ASS STAVSSSAEA A.KEQSKGQEL TEILSSTDWQ GTKX1YDKNXN NLTAENANFI GLAKYDCGETG ETRGDEGTFF VTDDGEKRIL ISDTQNYQAV VDLTEVTKDK FTYKRMGKOK HIPYSDEKLT FTNGRKDLET ETGKIVTNEP GDOILGATLW NGTKVLDEOG ISLA-KFDNKT SKYEFFDLET GKTRGDFGYF QVIDNNKIRA HVSIGDNKYG KRFTYTRMGK DNNGKEIKVF VEHEPYEGDF TPDFTF

FYEFFDKETG

DGKDVEVFXTE

NDVTEANKMF

AALELTELND

EF039-3 (SEQ ID NO:151) TGCAACAG ATAAATCAAG TGCAGCTAGC TCAAGCACTG CAGTCTCTAG TTCAGCAGAA 0 OS 0O 0*

GCAGCTAAAG

CAAGGCACAA

ATTGGTTTAG

GGTGAAACCC

TTAA'ITCGG

AAATTT1ACCT

GAACATATCC

ACAGAAACTG

TGGAATGGCA

TTTATTAGTT

ACGGGTAAAA

GCTCACG=F

GATAAACGTI'

TTTGTAGAAC

AGCAATCA-AA

AAGTTTACGA

CAAAATATGA

GTCGCGATGA

ATACACAAAA

ATAAGCGAAT

CTTATTCTGA

GCAAGA'ITGT

CGAAAG=TI

TAGCGAAATT

CACGTGGAGA

CAATT-GGTGA

TTACGTATAC

ATAACCATA

AGGACAAGAA

CAAAAATNAT

TGGTGAAACA

ACGCACATTC

CTATCAAGCG

GGGTAAAGAT

CCAGAAATI'A

TACCAATGAA

AGATGAAGAC

TGATAATA.AA

TTTTGGTTAC

CAATAAATAT

ACGAATGGGT

TGAAGGAGAC

AATAATTTAA

GGTTTTTATG

T'PIGTGACAG

GTGGTCGAIT

AAAGACGGGA

ACCTTrACGA

CCTGGCGATG

GGTAACGATG

ACAAGTAAAT

TTCCAAGTAA

GGAGCTGCAT

AAAGACAACA

TTTACGCCAG

CAGCAGAAAA

AATTTTTCGA

ACGATGGCGA

TAACGGAAGT

AAGATGTAGA

ACGGCCGTAA

ACAT=FAGG

rI'ACTGAAGC

ATGAA'ITCTT

TTGATAATAA

TAGAATflAAC

ATGGCAAAGA

ACTTCACGTT

TGCTAATTTT

CAAAGAAACA

AAAGCGTATC

GACGAAACAT

AGTCTTGTA

AGATTAGAA

GCCCACATTA

AAkATAAAATG

TGAITIAGAA

CAAAATCCGT

AGAATIAAAT

AATTAAAGTC

CTAA

TTAACAGAAA TTTATCCAG TACTGATTGG EF039-4 (SEQ ID NO:152) ATDKSSAASS STAVSSSAEA AKEQSKGQEL TEILSSTDWQ GTKVYDKNXN ETRGDEGTFF VTDDGEKRIL ISDTQNYQAV HIPYSDEKLT FTNGRKDLET ETGKIVTNEP ISLAKFDNKT SKYEFFDLET GKTRGDFGYF KRFTYTRMGK DNNCKEIKVF VEHEPYEGDF NLTAENANFI GLAKYDGETG VDLTEVTKDK FTYKRMGKDK GODILGATLW NGTKVLDEDG QVIDNNKIRA HVSIGDNKYG

TPDFTF

FYEFFDKETG

DGKDVEVFVE

NDVTEAkNKM'F

AALELTELND

EF040-1 (SEQ ID NO:153)

TAGATTAGAA

ATGAACA-AAA

TGTCAAGGAG

ACTAGTGCAA

GAGGAAGTGA

CAACGAGATA

CTCGACACAG

GCAAAAGCGT

CCCTATCTT

GCCACCCCAG

GAAAAAGGAC

TrCCAAAATG

CAACTTTACC

GACAATTCTG

CCACTGGAGA

AAATTAAT

GAGAAACACC

AAACTGAAAG

AAGAACCGAT

CGAAGAAGGT

AAGGCAACAC

TGATTGATCG

ATGCGGGAGC

ATTTAAATAA

ATATTGACAA

ACTTT1GGCTA

AAGATA=GT

GTCGAACAGC

AAAATCTCAT

GGGGCTA7TA

TTCCGCAGCG

CACCAGTACA

GAAGACCTAT

CAAGGAAATT

ACCGCTCAAT

GGGTGCCGAT

GCAAGGACGT

GCATAACCCT

TGTGAAGCTC

TACAGCATTG

AAAATM'TTA

AATGGACTAT

ATTTCTCTCG

AGTGTCGTGA

TCAAAAAATA

ACCCGTTCGG

GAAGTGGGTG

'ITACAAGATA

ATCGCTCTTC

ATTAATCTGC

ACGGAGATTT

TACGGTGGCA

rI'CTTAGAAG, A ITGAGGCAG

ATGGAAAATG

GCCAATCAA.A

AGGAAAGGAA

CCATI'CCATT

GTCAA.ACGGT

TAGCTCAAAC

CGCTTTAGA

CTACTTATCA

ACAATAATGA

AAAACAGCAT

TAGCGTATAT

ATGCGTTAAT

ATOGACCGA

TGGGGTACG

GTGCGGATCA

AAGGTTATAC

GTTGAGCACA

ACTTGCTGCG

GACTACTCAA

AACATCAAAA

AGCAGCCAAT

AGTGGATGAA

CATTGAGATTI

TAGTGATAGT

GTAAAACAT

TCCGGCAGCT

AGACATAGAT

TCAAGGGAAC

ATCCATTAAA

GGAAATTAGT

TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

AAAATTTTAG CACAGTACAA CTAA EF040-2 (SEQ ID NO:154) M NKKILMGLLS VVTIPLLAAC QGGETPSAAS KNSQTVTITQS SAXTESTSTT RSVAQTTSKE EVKEPMKlTYE VGALLEAANQ RDTKKVKEIL DTEGNTPLNI AVHNNDIEIA KALIORGADI NLQNSISDSP YIJYAGAQGRT TPDLNKHNRY GGNALIPAAE KCHIDNVKLL LEDGREDIDF QNDFOYTALI LYQDIVKLLM ENGADQSIKD NSGRTAMDYA NQKGYTEISK ILAQYN

QDTTYQVDEV

EILAYMLKHA

EAVGLREGNQ

EF040-3 (SEQ ID NO:155) AGCG TCAAkAAAATA GTCAAACGGT GACTACTCAA

AGTAGTGCAA

CAGGAAGTGA

CAACGAGATA

GTCGACACAG

GCAAAAGCGT

CCCTATCTTT

GCGACCCCAG

GAAAAAGGAC

TTCCAAAATG

CAACTTIACC

GACAATTCTG

AAACTGAAAG

AAGAACCCAT

CGA.AGAAGGT

AAGGCAACAC

TGATTGATCG

ATGCGGGAGC

A'I=AAATAA

ATATTGACAA

ACTTTGGCTA

AAGATA'ITGT

GTCGAACAGC

CACCAGTACA

GAAGACCTAT

CAAGGAA.ATT

ACCGCTCAAT

CGGGTGCCGAT

GCAAGGACGT

GCATAACCGT

TGTGAAGCTC

TACAGCATTG

AAAATTGTTA

AATGGACTAT

ACCCGTTCGG

GAAGTGGGTG

TTACAAGATA

ATCGCTGTTC

ATTAATCTGC

ACGGAGATTT

TACGGTGGCA

TTGTTAGAAG

ATTGAGGCAG

ATGGAAAATG

TAGCTCAPLAC

CGCTTTTAGA

CTACTT1ATCA

ACAATAATGA

AAAACAGCAT

TAGCGTATAT

ATGCGTTAAT

ATGGACGAGA

TGGGTT-ACC

GTGCGOATCA

AACATCAAAA

AGCAGCCAAT

AGTGGATGAA

CATI'GAGAT

TAGTGATAGT

C ITAAA.ACAT

TCCGGCAGCT

AGACATAGAT

TGAAGGGAAC

ATCCATTAAA

GGAAArAGT 0* @0 0 0@ .0 @0 0 0* 0* 0 0 96 @0 9* 0 000 9 00 0 0 000 0

S

*00000 0 0*@0 @000 00 @000 0 0 @000 *000 0S S 00 0S GCCAATCAAA AAGGTTATAC AAAAT'r=AG CACAGTACA.A C EF040-4 (SEQ ID NO:1SG) AS KNSQTVTTQS SAKTESTSTT RSVAQTTSKE EVKEPbETYE DTEGNTPLNI AVHNNDIEIA KALIDRGADI TPDLNKHNRY GGNALIPAAE KGHIDNVKLL LYQDIVKLLM ENGADQSIKD NSGRTAMDYA

VGALLEAANQ

NLQNSISDSP

LEDGREDIDF

NQKGYIEI SK RDTKKVKEI L QDTIYQVDE-V YLYAGAQGRT E ILAYMLXHA QNDFGYTAL I E-A\GLREGNQ

ILAQYN

EF041-1 (SEQ ID NO:157)

TAATTATTAA

ATGAAATrGA

GCGGCTTCTG

AGTCGCGAAC

CTAGCAACAG

'FrACACAAAG

GATGGACTAA

GTGACTGCTA

GAATATGCTr

GATAAATCAG

AAAGCAACAC

GACATTGTGG

GGTCCA'ITCG

AAAAACGATC

GTGAAAGAAT

CTTTCTGGTG

GCATCAACAC

NTETCTGATI'

AAAAGTCAT'

GAGGCGGCGG

AAGTTI'ACG

NCAGAAT'AG

ATAACAAAGT

CATACAAAAT

ATGACTATGT

ATCTGTATGC

AA~rAGGAAT

CATACTTTGA

AAAAATATGG

TCITAGACGG

AATATTGGGA

CACCAACCGC

AATTAGCCCA

AATATATGGA

TTCAGAAAAT

AACATTCGGT

AACGTCAGAT

TGTCACAGAA

'TTTATTGCA

CCAACCTGCA

TAAATTAAAT

TTACGGATGG

CTCTGTAAAA

TAAAGCAGTC

TTAC ITATTA

TAAAAA'ITAT

CTTTGATGGT

TAAAGATACT

GTTGAACTTG

ACAAATGGCC

ACTAAPATCAA

ACAGA'TGCA

GTGAT'ACAT

AGCTCAAGCG

CAACAAGA.AA

TTAAATAATG

GGTGCAGCGG

AAACATGCAA

CAACGAACAG

AATGGTGATG

AGTGATACAG

GCTTTCCCAT

GCATCAAACA

CCTGGTAcAG

GTGAAACTGG

TTCCAAGATG

AATOACCCAG

CGTGATGA

TTAI=TTAGG

TAT'IAGCGT

CGTCTGGTGG

TOCCAACAGC

TATATGAAGG

AAAAAGCAGA

AATGGTCAGA

TTGATCCAGC

CCATTGCTAA

AkATTAGAAAT CATTCTTr-CC

GCGAAAGTGC

ATACAAAATG

ACTCAGTAGA

GACAAACAGA

CTTTTGITAG

AATCACCATT

AGGCAACACT

AAcAACTTTA

CGGTAAGGCA

TGATTTATCA

AATTTATCGT

AGTTTCTGAA

CGGTAAACCA

GACAGCTTCT

AGGGGAAAAA

CACTTTAGAA

GCAACGTCAA

TG.TCTACAAT

GTC-ATTCAAG

TGTGAATGTC

CGATGTcGTT

TCAAAAAGPA

TAG-AAATGCG-

7, 120 TABLE 1. Nucleotide and Amino Acid Scqeuences of E. faecalis Genes.

AACTTACGTA AAGCAATTC TTACTCAATC CACCGTAAAG CGTTAGTTGA ATCAATCC'TT

AGGGGATGG

EF041-2 (SEQ ID NO:158) M KLKKSLTFGV ITLFSV~rLA ACGCGGTSDS SSASGGGKAS GEQVLRVTEQ QEMPTADLSL ATXRISFIAL NNVYEGIYRL DKDNKVQPAG GLTYKIKLNK DAXWSDGKPV TANDYVYGWQ RTVDPATASE YAYLYASVKN KSELGIKAVS DTELEITLEK ATPYFDYLLA FPSFF?QRQD) IVE(YUYA PFVLDGFDGP GTDTKWSFKK NDQYWDKIY' KLOS.VDVNVV KES PT-ALNLF SCELAQOMAN DPAFVSQKEA STQYMELNQR DEXSPFRNAN' LRKAISYSID

GW

AAEKAEVSED

GDAIAI(GEKD

SNSESAVYNG

QDGQTDDVVL

RKALVES ILR EF041-3 (SEQ ID NO:159) TTGTG GAGGCGGCGG AACGTCAGAT AGCTCAAGCG CGTCTGGTGG CGGTAAGGCA AGTGGCGAAC AAGTTTACG CTAGCAACAC NCAGAATTAG 6@ *0 S

SO

04 0@ 0 0@

OS

S

SS

.5 S S 0 @0 S S 000 0

S

@00500 0

SOS.

0 0000

TTAGACAA-AG

GATGGACTAA

GTGACTGCTA

GAATATGCTT

GATAAATCAG

AAAGCAACAC

GACA'FTGG

GGTCCATTCG

AAAAACGATC

GTGAAAGALAT

CTTTCTGGTG

CCATCAACAC

AACTTACGTA

AGGGGATGG

ATAACAAAGT

CATACAAAAT

ATGACT.ATGT

ATCTGTATGC

AATTAGGAAT

CATACTTTGA

AAAAATATGG

TCTTAGACGG

AATATTGGGA

CACCAACCGC

AATTAGCCCA

AATATATGGA

AAGCAATTTC

TGTCACAGAA

TTTTATTGCA

CCAACCTGCA

TAAATTAAAT

TTACGGATCG

CTCTITAAAA

TAA-AGCAGTC

TTACTTATTA

TAAAA.A'TAT

CTrTrTGATGGT

TAAAGATACT

GTTGAACTTG

ACAAATGGCC

ACTAAATCAA

'PrACTCAATC

CAACAAGA.AA

TTAAATAATO

GGTGCAGCGG

AAAGATGCAA

CAACGAACAC

AATGGTGATG

AGTGATACAG

GC7=1'CCAT

GCATCAAACA

CCGGTACAG

GTGAAACTGG

TTCCAAGATG

AATGAC C CAG

CGTGATGAAA

CACCGTA.AAG

TGCCAACAGC

TATATGAAGG

AAAAAGCAGA

AATGGTCAGA

TTGATCCAGC

CCA=GCTAA

AATTAGAAAT

CATTCTI'CCC

GCGAAAGTGC

ATACAAAATG

ACTCACTAGA

GACAAACAGA

CT=TTAG

AATCACCATT

CGTTAG=TA

TGATTTATCA

AATrATCGT

AGTTTCTGAA

CGGTAAACCA

GACAGCTTCT

AGGGGAAAAA

CACTTTAGAA

GCAACGTCAA

TGTCTACAAT

GTCATTCAAG

TGTGAATGTC

CGATGTCGTT

TCAAAAAGAA

TAGAAATGCG

ATCAATCC'Tr EF041-4 (SEQ ID NO:160) 606 5000

S

*000 0* 0 00 CGGGGTSDS SSASGGGKAS GEQVLRVTEQ QEMPTADLSL ATXRISFIAL GLTYKIXLNK DAKWSDGKPV TANDYVYGWQ KSELGIKAVS DTELEITLEK ATPYFDYLLA PFVLDGFDGP GTDTKWSFKK NDQYWDKD'IV SGELAQQMAN DPAFVSQK EA STQYMELNQR Gw EF044-1 (SEQ ID NO:161) NNVYEG IYRL

RTVDPATASE

FPSFFPQRQD

KLDSVDVNVV

DEKSPFR.NAiV

DKDNKVQPAG

YAYLYASVKN

IVEKYGKNYA

KES PTALNLF

LRKAISYSID

AAEKAEVSED

GDAIAKGEKD

SNSESAVYNG

QDGQTDDVVL

RKALVES ILR

TAAGATAAAA

ATTTTAGTTA

GAAAATTCTA

ATAGAAACAA

ACITCGTCAA

GCAACACCTT

GGAATGAATG

TTCCGAACTA

TTACTATAG

ITTTIAAC

CCAATCATAC

GCTCTAATAA

AGTCGAAAAA

ATGCTC.TCGA

TGCCAACTTC

A-ATT~GTTCo

CGTCTATAOG

AGGTI'GTAGT

AACATCAATA

TAAACTAAAA

TGAAGTATCT

TCTrAGTAGC

AATTACGTTA

GGCTGAAAAT

AGGAATAGTA

TCTCAAA.AAG

AAAAGCAGTA

GAAACTTcAG

ACAAATGTCG

TTAAACAATC

GAGAACTTAA

GGTCAAGTGA

TGAAAAATT

CGAATGAkACC

CTAATCATTA

A-AAGTGCCAG

A.AGAAGCAAA

CACTCGTATT

ATTCAACACC

AAGAAGCCAT

AGTTTGTGTT

TAAAAAACAA

CAGTTCTAGC

CACCACTCAA

TTCTI'TAGAA

TAATTITAAA

AAcTGCTACC

TAATAAATAT

121 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. fuecalis Genes.

GAGCTATCTA

AGTATTCGCA

GATGAACAAA

TTAATCACTC

GTAATTCAAT

TAAATACAAT TCCTACAAAA GAGATTAGAA TATITICAGC GGCCGATAAC CCGTTAAAGT AAATACAGAA TTAATI'TAG GALACTAATAT TTCTTAAAC ATAGATCGGG CACTTTATAC TTATTCAACA ATAAAAATGG TrCGATATCT CTAACTACGC TGGCAATGTT ACGGATG-ATC AAAAAGACGT TATGCTAGA-A

AA

EF044-2 (SEQ ID NO:162) MKKLVCVI LVIFLTGCSS QKANEPKKQE NSTNHITSIK SSTNHYSSSI ETSSNNKLKE TSESASTTQT SSKSKNEVST NVEEANSLEA TPYA'JDLSSL NNPLVFNFKG MNVPTS ITLE NLNSTPTATF RTXLFGAENG QVK-A INKYE LS INTl PTKE I RIFSAADNS IRTVKVNTEL ILGTNISSND EQNRSGTLYL FNN~kNGSISL ITPNYAGNVT DDQKDVMLEV

IQ

EF044-3 (SEQ ID NO:163) TTGTAGT TCTCAAAAAC CGAATGAACC TAAAAAACAA

GAAAAITCTA

ATAGAAACAA

ACTTCGTCAA

GCAACACCTT

GGAATGAATG

TTCCGAACTA

GAGCTATCTA

AGTATTCGCA

GATGAACAAA

TTAATCACTC

GTAATTCAA

CCAATC-ATAC

GCTCTAATA-A

AGTCGAAAAA

ATGCTGTCGA

TGCCAACTTC

AATTGT'ITGG

TAAATACAAT

CCGTTAAAGT

ATAGATCGGG

CTAACTACGC

AACATCAATA

TAAACTAAAA

TGAAGTATCT

TCTTAGTAGC

AATTACGTTA

GGCTGAAAAT

TCCTACAAAA

AAATACAGAA

CACT1'ATAC

TGGCAATG'T

AAAAGCAGTA

GAAACTTCAG

ACAAATGTCG

TTAAACAATC

GAGAACTI'AA

GGTCAAGTGA

GAGATTAGAA

TTAAITTTAG

TTA TCAACA

ACGGATGATC

CTAATCATTA

AA.AGTGCCAG

AAGA.AGCAAA

CACTCGTATT

ATTCAACACC

AAGAAGCCAT

TAT'LTCAGC

GAACTAATAT

ATAAAAATGG

AAAAAGACGT

CAG'ITCTAGC

CACCACTCAA

FrTTTAGA.A

TAATTTTAAA

AACTGCTACC

TAATAAATAT

GGCCGATAAC

TTCTTCAAAC

TTCGATATCT

TATGCTAGAA

EF044-4 (SEQ ID NO4:164) CSS QKANEPKKQE NSTNHTTSIK SSTNHYSSSI ETSSNNKLKE TSESASTTQT SSKSKNEVST NVEEANSLEA NNVPTS ITLE NLNSTPTATF RTKLFGAENG QVKEA INKYE IRTVKVNTEL ILGTNI SSND EQN'RSGTLYL FNNKNGS ISL

IQ

TPYAVDLSSL

LS INTIPTKE

ITPNYAGNVT

NNPLVFNFKG

IRIPSAADNS

DDQKDVMLEV

EF045-1 (SEQ ID NO:165)

TAGCCAA.AAA

TTAGCTTCTG

AATA.AACCAA

APLAGCAA771G

CTTTTCCAC

CAGCCATrAT

AAATI'AGATG

GACGGTAAAG

GACTATACAG

CATGATGGTA

ATCACTTATA

GTTTTACCAA

GrrCGTAAAA

TCTGI'TAAT

ATGAGGGAGG

TGTTAGTATT

CGGAAACAGA

ATGGCGGTAC

AAGAATTTTA

TTAACAATGA

AAGATGCCAA

ATGTGACAGC

GGATTCGTrA

AATCGCCAAC

AAGAAGTTCA

AACATG.CCT

ACCCTCTGAC

ACCTACCAAA

AAAAGAGATG

CGGGTTAGCT

AGACATTTCA

ATTAGATGTC

TCAAGACAAC

TGCAGACTTT

TACACCAACC

cGATGACGTG

TGATGATAAC

CATITCTGGC

CCCAGGAATG

TGAAGGAAT

TATTGGACCA

TGAGCATTAC

AACAAGAAAC

GCATGTGGTG-

AAAATGCCAA

GCTGTAGTTA

TATGATGCAC

AAGA'ITGTCG

ATrAAAT'AC ATTrCTCTT TTrACGAATA

ATAGAAAAAG

CAACAATTAG

GCTGTTAAAG

TACTACATGA

TACGGTGGTA

GGATTTTAGG

GCGGCAATAA

TCGCTGTTAA

TGGATACACA

AATACATGCT

ATGGGGGTCC

GTGACAATTT

ATGAAGTCAT

=TTTGGCAT

TCAATGATAA

GTGGCGGTGT

ACATGGAATC

GTAATATTGT

AACCTAAA-T

TGCAATCACG

AGGCGGGGGC

AAATGATAAA

ATTCCAAGGA

TCCAACGG-TA

TGCGGATCTG

GAAATGGTCT

TGGTCATAAA

GGAAGACTAC

AGAAGT'AAA

TFcGGGGCTCA

AAGCGATGCA

GACAGGTGAA

AGATAAATTA

122 TABLE 1. Nucleotide and Amino Acid Seqeuences of Efiaecalis Genes.

GTGTTCAAAT CTGTTCCTTC TGCGAGCATT ATTGCATTAT CAATGCCA-AC ACATACOTAT

ATCTTAGGAC

AAAGAAACAA

CAAGCCATGG

CGAACAGGGG

AAAGGCTATA

GACGTAGACG

GCTTCAATGT

AAAGAAATTG

TATCATAAAT

GGTTCAGATC

TTTGAGTCAG

GAAGAAAAAC

GTAATCCCAA

ACTTGGGCAG

GTCCCGAACA

ATACAGTGAA

GCTATGCAAT

CAACAACGTT

CGCTTGATT

GCGATGGCAT

CAGGCCGCGA

GCTTAAACGT

TGAAAAATGA

CTTCACCAAC

AAGAAAATAC

GTAAAAAAGC

CCCTTTACAG

TTGATACGA.A

AGCCTACACG

ATACAATCCA

TCACAATGAT

AATCCCACCA

AGACAAAGCG

TCGCGAAGAC

AACTGCACAA

PLACGTATACA

TGACCCAGAA

CGGCTTATAT

TAAAFI'ACTT

CTTCTACGAT

AAATGAAGTC

AGATAATCCA

GTAGAAGCGA TGAAAGCGAA CCAACATACA AAGATACTGA TATATTGGCT TTAAAATGGG AAAGCTAAAA TGGCAGATAA GCAGTCGGCC AA.AAATTCTA GTCTTCAAGA GCTTGCATGA AAAAAATTAT TAGACGATGC AAAGAAGGCA AACCACTAGA CCAC'ITGCTG ATTACTAT ACAGGACGCT TAATTGNI~r G-TAGATATCT ATCAAGGCGC GGfCcAAACT CACCT1=AA GATGCGATTG ATTCAAAAGC TGGCAAGAGT ATGCCATTGA TTGCCTGTCA ACGACCGTGT TGGGCAACGG TGGGTGTCAC

ACAATACGAT

AGGGTATCAA

TACGTTTGAC

AAGCTTACGT

CAACGGCTTA

TAGCGAAGCG

TGGTTATAAA

AATCAAGTTT

CCAACAATGG

CCAAGCATTC

GTGGGGCACA

CTATACACGT

ATCATTTGAT

TGAAGCGT

AGTI'GACTTT

AGCAGACTCA

CGGAAATAA

EF045-2 (SEQ ID NO:166) MN KKRILGAITL ASVL.VFGLAA CGGGNKGGGN KATETEDISK MPIAVKNDK

AIDGGTLDVA

LDEDANTATI

DGKSPTISG I

RKNPVTIGPY

ALSMPTDTYP

AMGYAIDNDA

VOGDGIREDK

DKLKNDDPEV

EKRKKAFYDW

K

VVMDTQFQGL

KLRDNLKWS D EKVNDKEVK I

YMSNIVTGES

TYKDTEGYQI

VGQKFYNGLR

EGKPLEIKFA

DIYQGAWGTG

QEYAIDEAFV

FQQEFYQDNY

GKDVTAIDDVI

TYKEVHPGMQ

'EYLPNEHYY

LGRPEQAYTY

TGATTLIPPV

SMSGGETAQP

SDPSPTGLYG

IPTLYRNEVL

DAQYNLPTVQ PLFNNDAE)FK IVDGGPADLK FSYEVIGHKD YTGIRYDE)NF TNIVGMEDYH

QLGGGVWGSV

GGKPKLDKLV

IGFKNGTFIDK

FKSLHDSEAK

LADYYVQQ WK

PNSAFNYTRF

PVNDRVVDFT

LPKHAFEG IA

FKSVPSASIV

ETNTVKYNPK

GYTLDLDKAK

EIGLNVTYTT

ESEENTKLLD

WAVDTKDNPW

EAMKAKQYDI

AKMADKSLRQ

KLLDDAGYKD

GRLIDFQAFY

AIDSKASFDE

ATVGVTADSR

EF045-3 (SEQ ID NO:167) ATGTGGTG GCGGCAATAA AGGCCGGGGC

AATAAAGCAA

AAAGCAATTG

CTTTTCCAGC

CAGCCAITAT

AAA'ITAGATG

GACGGTAAAG

GACTATACAG

CATCATGCTA

ATCACTTATA

GTTTTACCAA

G PTCGTAAAA

TCTGTTGAAT

GTGTTCAAAT

ATTGCATTAT

ATCITAGGAC

AAAGAAACAA

CAAGCCATGG

CGAACAGGGG

AAAGGCTATA

CGGAAACAGA

ATGGCGGTAC

AAGAATTTITA

TTAACAAT'GA

AAGATGCCAA

ATGTGACAGC

GGATTCGTTIA

AATCGCCAAC

AAGAAGTTCA

AACATGCCTT

ACCCTGTGAC

ACCTACCAAA

CTGTCCcTrC

CAATGCCAAC

GTCCCGAACA

ATACAGTLGAA

GCTATGCAAT

CAACAACCTI

CGCTTGAIT

AGACATI'TCA

ATTAGATGTC

TCAAGACAAC

TGCAGACTTT

TACAGCAACC

CGATGACGG

TGATGATAAC

CATTTCTGGC

CCCACGAATG-

TGAAGGAAI~T

TATTGGACCA

TGAGCATTAC

TGCGAGCATT

AGATACGTAT

AGCCTACACG

ATACAATCCA

TGACAATGAT

AATCCCACCA

AGACAA.AGCG

AAAATGCCAA

GCTGTAGTTA

TATGATGCAC

AAGATTGTCG

ATTAAATTAC

ATTTTCTCTT

TTACGAATA

ATAGAAAAAG

CAACAATI'AG

GCTGTTAAAG

TACTACATGA

TACGGTGGTA

GTAGAAGCGA

CCAACATACA

TATATTGGCI

AAAGCTAAAP

GCAGTCGGCC

GTCTITCAAGP

TCGCTGITAA

TGGATACACA

AATACATrGCT

ATGGGGTCC

GTGACAATT

ATGAAGTCAT

'TGTTGGCAT

TCAATGATAA

GTGGCGGTGT

ACATGGAATC

GTAATATI'GT

AACCTAAATT

TGAAAGCGAA

ALAGATACTGA

TTAAAATGGG

TGGCAGATAA

AAAAATTCTA

LGCTTGCATGA

AAATGATAAA

ATTCCAAGGA

TCCAACGGTA

TGCGGATCTG

GAAATGGTCT

TGGTcATAAA

GGAAGACTAC

AGAAGTTAAA

TTGGGGCTCA

AAGCGATGCA

GACAGGTGAA

AGATAAATTA

ACAATACGAT

AGGGTATCAA

TACGTTGAC

AAGccTACGT cAAcGGCTTA

TAGCGAAGCG

AAAAAATTAT TAGACGATGC TGGTTATAAA 123 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

GACGTAGACG

GCTTCAATGT

AAAGAAAI'rG

TATGATAAAT

GG~TTCAGATC

TTGAGTCAG

GAAGAAAAAC

GTAATCCCAA

ACTTGGGCAG

CGGAAA

GCGATGGCAT

CAGGCGGCGA

GCTT~AAcG.T

TGAAAAATGA

CTTCACCA.AC

AAGAAAATAC

CTAAAAAAGC

CGC'ITTACAG

TTGATACGAA

TCGCGAAGAC

AACTGCACAA

AACGTATACA

TGACCCAGAA

CGGCTTATAT

TAAATTACTT

CTTCTACCAT

AAATGAAGTC

AGATAATCCA

AA.AGAAGGCA AACCACTAGA AATCAAC'IT CCACTTGCTG ATTACTATGT CCAACAATGG ACAGGACGCT TAAzTTGATI'T CCAAGCATTC GTAGATATCT ATCA-AGGCGC GTGCGGC-ACA GGTCCA.ACT CAGCC'F=AA CTATACACGT GATGCGATTG ATTCAAAAGC ATCATTTGAT TGGCAAGACT ATCCCATTGA TGAAGCTT TTGCCTCTCA ACGACCGTGT ACTTC.ACTT1 TGGGCAACGG TGGGTGTCAC AGCAGACTCA EF045-4 (SEQ ID NO:168)

CGGGNKGGGN

Al DGGTLDVA

LDEDANTATI

DGKSPTISGI

RKNPVTIGPY

ALSMPTDTYP

AMGYAIDN'DA

VDGDGIREDK

DKLKNDDPEV

EKRKKAFYDW

K

KATETED ISK

VVMDTQFQGL

KLRONIJKWSD

EKVNDKEVKI

YMSNIVTGES

TYKDTEGYQ I

VGQKFYNGLR

EGKPLEIKFA

DIYQGAWGTC

QEYAI]DEAFV

MP IAVKNDKK

FQQEFYQDNY

GKDVTADDVI

TYKEVHPGMQ

VEYLPNIEHYY

LGRPEQAYTY

TGATTLIPPV

SMSGGETAQP

SDPSPTGLYG

IPTLYRNEVL

DAQYMLPTVQ

FSYEVIGHKO

QLGGGVWGSV

GGKPKLDKLV

IGFKMGTFIDK

FKSLHDSEAK

LAIDYYVQQWK

PNSAFNYTRF

PVJNDRVVDFT

PLFNNDADFK

YTC IRYDDNF

LPKHAFEGIA

FKSVPSAS IV

ETNTVKYNPK

GYTLDLDKAK

E IGLNVTYTT

ESEENTKLLD

WAVDTKONPW

IVOGGPADLK

TNIVGMEDYH

VKDMESSDAV

EAMKAKQYDI

AKMADKSLRQ

KLLDDAGYKD

GRLIDFQAFY

AIDSKASFDE

ATVGVTADSR

EF046-1 (SEQ ID NO:169)

TAGGAGGATA

TGTAGTAATA

CAGCAAkACTA

ACATCATCTA

GATGATGTT

ACGGATAAAG

AATTATATTA

TAATGAAAAA

ATACTGGGGG

CCCAGTCTTC

TAACAATTGA

CAAAAACTAG

AACTAAAGGA

AGCAAAAA

AAAACTTATT

AAAAAATAGC

TAAAAAAGAT

AACAACCGAG

ACGACAATTG

ATATATATCA

GTACTATTGT

GACGCTTCAT

AGTAGTAATC

AATTTAAAGA

TATGAACAAG

GAGGCTAAAG

TAGCC'Aq-'I

CTACTGAAGT

CGGACACAAC

ATAGAGAATT

GAATTAACAG

AACAAAGPA

TTTAACGGCA

ATCAACTAAG

ACcAAcTTCT

GAATCCAACA

TTCAACAATT

AGATGTCAT'

EF046-2 (SEQ ID N4O:17O) MKKKLIV LLLALFLTAC SYNTGGKNSD ASSTEVSTKQ QTTrQssKKDS SNPDTTPTST SSITIETTEN LKNRELNPTD DVSKTRRQLY EQGINSST IT DKELKEYISE AKEQKKDVIN YI4QK EF046-3 (SEQ ID NO:171)

A

TCTAGTAATA

CAGCAAACTA

ACATCATCTA

GATGATGT

ACGGATAAkAG

AATTATATTA

ATACTGGGGG

CCCAGTCTTC

TAAcAATTGA cAAAA.ACTAG

AACTAAAGGA

AGCAAAAA

AAAAAATAGC

TAAAAAAGAT

AACAACCGAG

ACGACAATTG

ATATATATCA

GACGCTTCAT

AGTAGTAATC

AATTTAAAGA

TATGA.ACAAG

GAGGCTAAAG

CTACTGAAGT

CGGACACAAC

ATAGAGAAT

GAATTAACAG

AACA.AAAGAA

ATCAACTA.AG

ACcAAcTTCT

GAATCCA-ACA

TTcA.AcAATT

AGATGTCATT

TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

EF046-4 (SEQ ID NO:172) C SNNTGGKNSD ASSTEVSTKQ QTTQSSKKDS SNPDTTPTST SSITIETTEN LKNRELNPTD DVSKTRRQLY EQG INSST IT DKELKEYI SE AXEQKKDVIN

YIKQK

EF047-l (SEQ ID NO:173)

TAGGGAAAAC

ITA.ACCCTTG

TCCAGTGCTG

AGTAGCAAAG

TCGAAAGCAA

TCACCAGCGA

AACATGTTTC

TTAACTGCAG

GAAAAAGAAG

=TGAGAAAA

GACAATGGCG

GCAGGTTCTA

AATATCAATG

GAAACATTAC

GACTATAATC

AAGGAGGAAT

CTTTAGCAGC

CGGAAAGTAC

CAACAACAAA

CAGCT1'CTTC

AAAATCGCGAA

CTGGTCAAGG

CGACAACTTC

CGATTCCAGT

AAACATATGG

GTCAACCAGT

GTIACITATC

GTG-AATCCC

CAGCACCGAA

GAAACTCAGT

TCTTATGAAA

ATGCGGCGGC

CACAGTCGAG

ATCTAGTGAT

TACGAAGGAA

TCCAGATGAC

CTTACCCCAG

ACAAGCGGAT

GAATGATGCA

CTCTGATCC

AGATTAGGT

TTGGCAAGAA

TGATGATTTA

TACCGTTGGT

AGTTTGGCAA

AAGATAGGGC TTA=TCTAG TGCTrPTICTT

GGAAAAAGTA

AGTACAAAkAG

GCGAAACCGT

GCGGCAAATA

CAAGCCAACC

GCAATI'TPJ

CAAAACAATT

CGTGTCAATC

GAAGCAAAAA

TACAATATTA

CAGAAAATAC

CATCTCCTAC

CAGGAACAAC

ATGGCTCAGC

AAGTCTAA

CGAGTCAAAC

TCCGTGT=T

AGTTAACGCC

ATGCAGTGAA

CTGGG'-TATAA

GGATAGTCGT

AAAAGAATCA

AACACCTGAT

AGAGAAGCAA

CCAGCTAGCA

GAATAACTT

ATATTATGCA

AATTAG'ITCT

CCAAATCATT

ACAAGGGGCG

ACGGGCCTCA

TTCGAACAA

AGGAACCACT

TGTCCATCAT

GGCAAT'TGGA GTTTAGTCGT GCGAAAAATG TTGTCAACAT CAAATCACAC TGAACGTGGC GCCGGTACAG TCGTT--ACTC ACATCAATGT AA TTTGACCCAA TTCAAGCAGT GAAGATGGCA EF047-2 (SEQ ID NO:174) IMflK IGLISSAFLL T7ALAACGGG KSTENTDSRS SAAESTTVES TKASATKESS SKATTKSSDA KPSGTTTADS KATASSTKEA AN~NGSAEKQS PAKNANPDDQ ANQVLNQLAN MFPGQGLPQA ILTSQTNNFL TAATTSQADQ NNFRVL.YYAE KEAI PVNDAR VNQLTP ISSF EKKTYGSDAE AKNAVNQIID NGGQPVDLGY NITGYKQGAA GSSYLSWJQEG NWSLVVR-ASN INGESPDDLA KNVVNILEQE TLPAPNT-VGQ ITLNVAGTTD YNRNSWVWQA GTVVYSVHHF DPIQAVKMAT SM EF047-3 (SEQ ID NO:175)

ATGCGGCGC

TCCAGTGCTG

AGTAGCAAAG

TCGAAAGCAA

TCACCAGCGA

AACATGT'TC

TTAACTGCAG

GAAAAAGAAG

TTTGAGAAAA

GACAATGC

GCAGGTTCTA

AATATCAATG

GAAACATTAC

GACTATAATC

'TTGACCCAA

GGAAAAAGTA

CGGAAAGTAC

CAACAACAAA

CAGCTTCTTC

AAAATGCGA-A

CTGGTCAAGG

CCACAACTTC

CGATTCCAGT

AAACATATGG

GTCAACCAGT

GTTACTTATC

GTGAATCGCC

CAGCACCGAA

GAAACTCAGT

TTCAAGCAGT

CAGAAAATAC

CACAGTCGAG

ATCTAGTGAT

TACGAAGGAA

TCCAGATGAC

CTTACCGCAG

ACAACGAT

GAATGATGCA

CTCTGATGCC

AGATTTAGGT

TTGGCAAGAA

TGATGATTTA

TACCCI'GGT

AGTTTGGCAA

GAAGATGGCA

CGATACTCGT

AGTACAAAAG

GCGAAACCGT

GCGGCAAATA

CAAGCCA.ACC

GCAATTTTAA

CAAAACAATT

CGTGTCAATC

GAAGCAAAAA

TACAATATTA

GGCAATTGGA

GCGAAAAATG

CAAATCACAC

GCCGGTACAG

ACATCAATGT

CATCTGCTAC

CAGGAACAAC

ATGGCTCAC

AAGTGCTrAA

CGAGTCAAAC

TCCGTCTTTT

AGTTAACCCC

ATGCAGTGAA

CTGGGTATAA

GTTTAGTCGT

TTGTCAACAT

TGAACGTGC

TCCTTIACTC

AA

AAAAGAATCA

AACAGCTGAT

ACAGAAGCAA

CCAGCTAGCA

GAATAACTTT

ATATTATGCA

AATTACTTCT

CCAAATCT

ACAAGGGGCG

AcGGGCCTCA TrTGGAACAA

AGGAACCACT

TGTCCATCAT

EF047-4 (SEQ ID NO:1?76) 125 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

CGGG KSTENTDSRS SAAESTTVES TKASATKESS SKATTKSSIJA KPSGTTTADS KATASSTYE-A ANNGSAE-KQS MFPGQGLPQA ILTSQTNNFL TAAMTSQADQ NNFRVLYYAE EKXTYGSDAE AKNAVNQIID NGGQPVDLGY NITGYKQGAA INGES PDDLA KNVVNI LEQE TLPAPNTVGQ ITLNVAGTTD DPIQAVKMAT SM PAKNANPDDQ ANQVLNQLAN KEAIPVNDAR VNQLTPISSF GSSYLSWQEG NWSLVVRASN YNRNSWVWQA GTVVYSVIIXF EF048-1 (SEQ ID NC:177)

TA.AGGAGAPA

GGATTACAGT

TTGCGCTATG

CAATTAGTAG

TTAGGAGGAG

GTCAGTGGAT

ATTITTTGATA

GTGTATGATT

CGAAGTTTT

ATTCGGGTCA

GTACCTATGGC

GAGAATAATG

TATGATGAGC

TTGACAGCGA

AAAACGGTCT

GTTGTGTTCA

TCATTCAAAA

AGTTCATGAA

TGCTA-AGTGG

CGTATGCTAG

AGGAAAAAAC

AAACAGAACT

CAGCATTAGA

ATGAAAAACA

CTACAAAAAA

ATATGAGCAA

TGCAAAGTGA

GTAGI-TCGGA

AGTTCGTI"IT

ATACTCGAGT

AACAAGAACA

TTAAAGAAGC

ATCAAGTAGA

ATACCTCAGA

AAA;AGAAAG

TGCGGCAAA

TAATAGCCAA

AGATGGTAAA

AATTrGAATTA

AAG=ITCT

TTTTAAA

ATTAGGATT

AGGGCCTGTT

AACCGCCATC

AGTATATACT

ATATACAGCT

GCCAGATATT

AGCGATTGAA

GGTTGAAGAA

CAGTGAACCA

ACATGGCGAA

G'I'TTATTTA

ACAGAAGCTT

CCAGI'ATCG

GTTCAAATTG

ACACAAACAG

AAAGATTATT

GTAATGGATA

GTTGCTI'TAA

ACATCTCCAG

AAAATGGTAG

TCTCTACA.AT

GGTCATGGTG

GTGATTATGA

GAACCAGCAA

GAAAAGAAAA

TTCCAAAAAC

CTGTATCAGG

CAGCAGTTAT

CGGCAAATGA

ATTCTATGAA

AATKTITCC

GTrGCAATTGA CTGTATtGC

ATCAAGCGCT

CTITATTATGA

ATGA=TGAA

A.ACT=~AGG

CTA.ATCTAAT

GTGTGCCTAA

ACGAGGGAAC

AAGAATCGAC

AAGCACAAC

TTGTTCAACC

CTATTCGCCA

GGTATTGGCA

TACGGTAGTC

GAALATTCGGT

AGATGGTCAA

TTTTGCAAAG

CATTCCGTAT

AATGCAACCA

CTCTGGTCAA

AGGTAAAA.AA

GGGTTCGCCA

CAACCGTGCA

GTATIATTCT

AAAAGAACGT

CGCTTTTGAA

AGAATATGGC

GTTGCATGAA

GTTGCCGGAC

EF048-2 (SEQ ID NO:178) MKKRKV LFTAVM4VLAG LQLLSGCGKT EASANDTVVL RYAYASNSQP VIDSM(KFGE LVEEKTDGKV Q IEYFPDGQL FONEKHFFKV NDNQALMQPV RVMQSETAIK MVELLGGSPV DEHTRVPDIV IMNEGTKERL VFNQVDSEPF QKLVQPLHES

GGETELIELT

YDSTKKLGFV

PMGSSEVYTS

TAKQEQAIEE

FKNSSEHGEL

QTGAI DFAKV

GLTYYDSGQR

LQSNLINGAE

AAKESTAFEK

YQAIRQLAD

SGSALESFSK

SFYMSKGPVT

NNEFVLYTAG

TVFKE-AVEEE

DYSVFAIPYI

SPDDLKGKKI

HGGVAKYYSY

KKKAQAEYGV

EF048-3 (SEQ ID NO:179)

TTGCGGCAAA

TTGCGCTATG

GAATTAGTAG

TTAGGAGGAG

GTCAGTGGAT

ATTTTTGATA

GTGTATGAWT

CGAAGTI=T

ATTCGGGTCA

GTACCTATGG

GAGAATAATG

TATGATGAGC

ETGACAGCGA

ACAGAAGCTT

CGTATGCTAG

AGGAAAA.AAC

AAACAGAACT

CAGCATTAGA

ATGAAAAACA

CTACAAAAA-A

ATATGAGCAA

TGCAAAGTGA

GTAGTTCGGA

AGTTCGT=

ATACTCGAGT

AACAAGAACA

CGGCAAATGA

TAATAGCCAA

AGATGGTAAA

AAI'rGAATTA.

AAGTTTCT

TTTTTAAA

AT'rAGGATTT

AGGGCCTGTT

AACCCCATC

AGTATATACT

ATATACAGCT

GCCAGATATT

AGCGATTGAA

TACGGTAGTC

CCAGTTATCG

GTI'CAAATTG

ACACAAACAG

AAAGATI'ATT

GTAATGGATA

G TGGTTTAA

ACATCTCCAG

AAAATGGTAG

TCTCTACAAT

GGTCA=GTG

ATTCTAMAA

AATATTITCC

GTGCAATTGA

CTGTAITIC

ATCAAGCGCT

CTTATI'ATGA

ATGAT7IGAA AAC TTIrAGG

CTAATCTAAT

GTGTGGCTAA

GAAAT1'CGGT

AGATGGTCAA

TTrTGCA;AC

CATTCCGTAT

AATGCAACCA

CTCTGGTCAA

AGGTAAAAAA

GGGTTCGCCA

CAACGGTGCA

r TArATICT GTGATTATGA ACGAGGGAAC AAAAGAACGT GAAGCAGCAA AAGAATCGAC CGCTTI'GAA 126 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

AAAACGGTCT TTAAAGAAGC GG ITGAAGAA GAAAAGAAAA AAGCACAAGC AGAATATGGC GTTGTGTCA ATCAAGTAGA CAGTGAACCA TI'CCAAAAAC TTGTTCAACC GTTGCATGAA TCATTCAAAA ATAGCTCAGA ACATGGCGAA CTGTATCAGG, CTATTCGCCA GTTGGCGGAC

TAA

EF048-4 (SEQ ID NO:180) CGKT EASANDTVVL RYAYASNSQP VIDSMICFGE LVEEKTDGKV QIEYFPDGQL GGETELIELT QTGAIDFAKV FDNEKHFFKV MDNQALMQPV YDSTKKLGFV GLTYYDSGQR RVMQSETAIK HVELLGGSPV PMGSSEVYTS LQSNLINGAE DEHTRVPDIV IMECTKERL TAKQEQAIEE AAXESTAFEI( VFNQVDSEPF QKLVQPLHES FKNSSEHGEL YQAIRQLAO

SGSALESFSK

S FYM SKG PVT

NNEFVLYTAG

TVFKEAVEEZ

DYSVFAIPY I

S?DDLKGKKI

HGGVAKYYSY

KKKAQAEYGV

EF049-1 (SEQ ID NO:181)

TGAGACTCTT

AAAACAGGAG

GCCGT'ITTTA

TCAACAATTA

GCGTTAGCC

GACAAAGANA

AAGTTCTCTG

AAACAAAGAG

TTAAAAACAG

TICAGAAGATG

ATTGCTAAAG

GATTCACAAG

GGCGAAGTGT

GTAGTGAAAA

ATCAAGAAAA

AGTGACGAAT

GCAGGCTACA

AGTGATTCTA

AGCAAAACAA

TCTTT=~AA

TGCATAAGAG

GTTTAGCAGC

CTGTTGATGA

AAATGTAT

TTCAAAAAAA

ATGCATTAAA

CAGCCTATGA

C'TGGGCAAG

ATGCCAAAGC

AAAAATCAAC

CAACAACTGT

CAGAACCAAT

TGACGAAAAA

TTGCTGAAGA

TAA.AAGCGGC

TGCAAACTGA

AAACAAGCGA

CAGAATCTTC

AATGAGGTAT

AATGAAGAAA

GTGTTCAAGC

TTTTTATAAC

TTATA-AATC

C LTGACGAA

ACAAGCTGGT

TGCAGGTCTA

TTTCCATCCA

TGTCAAGAAA

AGATACTGCT

TCCTGCCGAA

TGCTGCAACA

CAAAGCAAAA

AACAAAATTA

CAATGTGAAA

ATCTTCAAGC

TACCAAAACA

TTCTAALATAA

GGTATAGTTA TAACAGANAT AAAACTANAA AAACTAATCT TAGCTGCAGC GGGCGCAATG

GGTTCAAAAG

CAAATTAAAG

TTTGAAGAAA

GCCAAAGAAC

TTAACTGAAA

AAAGCCCACT

GAAGTAGAAG

GAAATCACTG

ACCAAAAAAG

GTTAAAGA.AG

AATATGCAAA

GGCAATGACA

GCCGATCAAA

ATTAAAGATG

GCTTCTTCAG

AGCGACTCT'G

ATATCGCAAC

AACAAAGCAC

AATATGGCGA

AAGTAGAAGC

AAACATTCAA

TAAAAATTAC

CACAAATTAT

ACGGCGGCGA

ATGGCOCTAA

CTGCCTTCA.A

CCTACCA.AAC

TGAAACCTTA

CATTIvrTTC

ATGCCTTCAA

AGAAAAAAGA

AAAAAGCAAC

AATGAAAGGT

TAGCCAACAA

CAAAGTAACT

ACAAGGCGGA

GAAACAGTTA

AGATGAAGAC

CCAAGTTGCT

TTTCACAAAA

AATTAAATTT

ATTAAAAGAT

AACCTACTAT

TGAAAAAGAC

GAA.AGTCATT

GAACGCTTTA

ATCAAAATCA

AGATI'CTTCA

EF049-2 (SEQ ID NO:182) MKKK LILAAAGAMA VFSLAACSSG SKDIATMKGS TITJDDFYNQ IKEQSTSQQA FSQMVIYKVF E:EKYGDKVTD FSDALKQAGL TEKTFKKQLK QRAAYDAGLd( AHLKITDEDL EDDAKAVKKE ITDGGDFTKI AKEKSTDTAT KKDGGKIKFD EVSEPIAATN MQTYQTTYYV VKMTKNYKC NDbM(PYEKEI DELKAANVKI KDDAFKNALA GYMQTESSSA SSEXKESKSS

KTTESSSK

KXIQKNFDEA

KTAWASFHPE

SQATTVPAEV

KKIAEET KLA

DSKTSDTKTS

KEQVEAQGGK

VEAQI IQVAS

KEAAFKLKDG

DQTFVSKVIS

DS EKATDSS S EF049-3 (SEQ ID NO:183)

GTGTTCAAGC

TCAACAAITA

GCGTTTAGCC

GACAAAGANA

AAGTTCTCTG

GCTICAAAAC

CTGTIGATGA

AAATGGTTAT

TTCAAAAAAA

ATGCATTAAA

ATATCGCAAC

ITT'TTATAAC

TrATAAAGTC

CITI'GACGAA

ACAAGCTGGT

AATGAAAGGT

CAAATTAAAG

77TGAAGAAA

GCCAAAGAAC

7r'AACT.AA

AACAAAGCAC

AATATGGCGA

AAGTAGAAGC

AAACATTCAA

TAGCCAACAA

CAAAGTAACT

ACAAGGCGGA

GAAACAG'PrA 127 TABLE 1. Nucleotide and Amino Acid Seqeuetices of E.f[aecalis Gcncs.

AAACAAAGAG

TTAAAAACAG

TCAGAAGAG

ATTCCTAAAG

GATCACA-AG

GGCGAAGTGT

GTAGTGAAAA

ATCAAGAAAA

AGTGACGAAT

GCAGGCTACA

AGTCA 1TCTA

AGCAAAACAA

CAGCCTATCA

CTTGGGCAAG

ATGCCAAAGC

AAAAATCAAC

CAACAACTGT

CAGAACCAAT

TIGACGAAAAA

TTCCTGAAGA

TAAAAGCGGC

TGCAAAC TGA

AAACAAGCGA

CAGAATCTTC

TGCAGGTCTA

T='ICCATCCA

TGTCAAGAAA

AGATACTGCT

TCCTGCCGAA

TGCTGCAACA

CAAAGCAAAA

AACAAAATTA

CA.ATGTGAAA

ATC'ITCA.AGC

AAAGCCCACT

GAAGTAGAAG

GAAATCACTG

ACGAAAAAAG

CTTAAAGAAG

AATATGCAAA

GGCAATGACA

CCGCATCAAA

ATTAAAGATG

GCTTCTTCAG

TAAAAATTAC

CACAAATTAT

ACGGCGGCGA

ATGGCGGTAA

CTGCCTI'CAA

CCTACCAA-AC

TGAAACCTTA

CATTTG.7'TC

ATGCC~TCAA

AGAAAAAAGA

AAAAAGCAAC

AGATGAAGAC

CCAACTTGCT

'rTCACAAA.A

AATTAAATTT

ATTAAkAAGAT AAC CTAG TAT

TGAAAAAGAG

GAAAGTCATT

GAACGCTTTA

ATCAAAATCA

AGATTCTTCA

TACCAAAACA ACGACTCTC

TTCTA-AATAA

EF049-4 (SEQ ID NO:184) GSSG SKDIATMKGS TITVDDFYNQ IKEQSTSQQA FSDALKQAGL TEKTFKKQLK EDDAKAVKKE ITDGGDFTKI EVSEPIAATN MQTYQTTYYV DELKAANVKI KDDAFKNALA

KTTESSSK

FSQMVIYKVF

QRAAYDAGLK

AKEKSTDTAT

VKMTKNKAKG

GYMQTESSSA

EEKYGDKVTD

AHLK ITDEDL

KKDGGKIKFD

NDMKPYEKE I

SSEKKESKSS

KXIQKNFDE.A

KTAWASFHPE

SQATTVPAEV

KKIA=EETKLA

DSKTSDTKTS

KEQVEAQGGK

VEAQI IQVAS

KEAAFKLKDG

D)QTFVSKVIS

OSEKATDSSS

EFOSO-1 (SEQ ID NO:185)

TAGGGTCTGG

AAAGGATGNA

CTTGTTCTAT

GCCACAAAAG

GGTI'ACGCAG

GCCACAACTC

GGAGCTTAA

AACAATGAAT

TATCAAGTGA

TATTTAGGAA

GGAGCACCTT

GAAAACGGCC

GTTCCAGAPA

GATGGA-ACGG

ACTCAAGTCA

AATCCTGAAG

AAAAGCAGTC

AAAAAATGAA

TATTA-AGTGC

AAGCAACTGT

GAACTAAAAA

AAGAATTAGT

GTGGCAAAC

GGAATCAAAC

ACAAAGCCAA

CCTATGGGCA

TCAATGATTT

CCACAGAAAA

AACTGCCCAT

TGCCGTTATC

CTAGCCAGAT

TAGATCAATT

AAGTGACTTC

CATGCCCAA-A

TTGCCAAATT

TCAGTTAAAC

TTCGTTTGGC

GCTACTCGTT

GACGAATCCC

AGAATGGATA

TATTGTCGGG

AGATACATCG

TATTGATACG

AAGTAGCG

TTATTAATT

TAGTGCCTTA

TATTAAAGGA

GCTAATCGAA

TITCCAAGG

AATATCNGTT

GGGGCAACTA

CGCACAACAA

TCGTTACTGC

AAACCTGATG

AGTGTTCAAG

AAAAACACAT

CACTCTATGG

TrACCTAAA

ACTCAACAGC

TATTGGATT

GGTGGTCAAT

GCAGTCAACG

GAAAATGCAC

TI'CTATGGC

CCTT'ITIAG

ATTTITrC~T

CGAAGGATGA

CACCAAGT

ATCGCTTGGA

GGACCGTGGT

TTCTAT1GA

TACTCTATTT

GTGGTGITAG

TTGAAAAA?1'

AAACCATCGA

ATCA.AGAGAT

TAAGTCCAAC

CCTTGCTA.AG

AACATAGTCA

CGAGTAAAAA

TTCATGGCAG

GCTAATGGGT

CAACCAAGCC

TTTTTCAT

GAAACAAGGT

TAAAGAGCGA

AGATAATAAA

ACAAAAAAAT

TGGTIACGT

CGTCAGCATT

AACGGAACTA

GATTAATGTT

AGAT ITAAGT

ACAGCGAGGA

ATI'ACATGAA

ATAG

EF050-2 (SEQ ID NO:186) MNMPKN IXYFSLLMGL VLLLSACQIG ATTKDDNSQAA TKEAIVELNR 'FrTPTLFFHG YAGThNSFGS LLHRLEKQGA TTQELVLLNKy ALSGKATNPS VQVLFEDNKN NEWNQTEWIK NTLLYLQKNY QXTNKANIVGH LGTYGQDTSL PKIEKFVSIG APFNDFIDTS QQQTIETELE NGPTEKSSRY PEKLPILLIG GQLSPTDLSD GTVPLSSALA VNALLRQRGT QVTSQI IKGE PEVDQLLIEF LWPSKX pDCGTVKERG

SMGVSGLRY

LDYQEMINVV

NAQHSQLHEN

EF050-3 (SEQ ID NO:187) 128 TABLE 1. Nucleotide and Anlino Acid Seqeuences of E.faecatis Genes.

TTGCCAAATT

GCCACAAA.AG

GGTTACCCG

GCCACAACTC

GGAGCT'TTA.A

AACAATGAAT

TATCAAGTGA

TATTTAGGAA

CGAGCACCTT

GAAAACGGCC

GTTCCAGAAA

GATGGAACGG

ACTCAAGTCA

AATCCTGAAG

GGGGCPLACTA

AAGCAACTGT

GAACTAAAAA

AAGAATTAGT

GTGGCAAACC

GGAATCAA-AC

ACAAAGCCAA

CCTATGGGCA

TCAATGATTT

CCACAGAAAA

AACTGCCCAT

TGCCGTT'ATC

CTAGCCAGAT

TAGATCAAT

CGAAGGATGA

TGAGTTAAAC

TTCGTTTGGC

GCTACTCGTT

GACGAATCCC

AGAATGGATA

TATTGTCGGG

AGATACATCG

TA=TATACG

AAGTAGCCC

TTTATTAATT

TAGTGCCTTA

TATTAAACCA

GCTAATCGAA

CAACCAAGCC

CGCACAACAA

TCGTTACTC

AAACCTGATG

AGTGTTCAAG

AAAAACACAT

CACTCTATCG

TT1ACCTAAAA

AGTCAACAGC

TAT'rrGGA'Tr

GGTGGTCAAT

GCAGTCAACG

GAAAATGCAC

TWICTATGGC

CACCAACGCT

ATCGCFTTGA

GGACCGTGGT

TTCTATTTGA

TACTCTATTr GTrCGTGTTAG

TTGAAAAATT

AA.ACCATCGA

ATCAAGAGAT

TA.AGTCCAAC

CCTMCTAAG

ALACATACTCA

CGAGTAAAA-A

TTrTTTTCAT

GAAACAAGGT

TAAAGAGCA

AGATAATAAA

ACAAAAAA.AT

TGGTITACGT

CGTCAGCATT

AACGGAACTA

GATTAATGTT

AGATTTAAGT

ACAGCGAGGA

ATTACATGkA

ATAG

EFOSO-4 (SEQ ID NO:188) 9* 9 9* CQIG ATTKDDNQAA TKE-ATVELNR TTTPTLFFHG ALSGKATNPS VQVLFEDNKN LGTYGQDTSL PKIEKFVSIG PEKLPILLIG GQLSPTDLSD PEVDQLLIEF LWPSKK

YAGTKNSFCS

NEWNQTEWIK

APFNDFIDTS

GTVPLSSALA

LL]HRLEKQGA

NTLLYLQKNY

QQQTIETELE

VNAL.LRQRGT

TTIQELVLLVK PDGTVVKERG QVNKANTIVGH SMGCVSGLRY NGPTEKSSRY LDYQEMINVV QVTSQIIKGE NAQHSQLHEN EF051-1 (SEQ ID NO:189) 9 a.

TAAAAG;AAAA

TTCAGTTTAG

ACAAAAAAAG

AAAATTAT'C

AGTTCTGGAA

GCTTCGACAA

CTTCCTTTAT

CATCATTTI'

CCAGCTGGTC

AAACACGCAA

GCAGAAGCTrG

GCGCCCATGC

GCCTCTAAGA

AGAAAATATT

CGGCGTTCA

TTGCCCTAAT

AAGAAATAAC

CAGCCTTTGA

AATI'ACAGAT

AACAAATGAA

TGGAAAACCA

CTGAT'TAA

AATATGCCGA

GCTITGGCAC

GCTTACTTTA

CTGAAGCTT

AACAAAAATC

TTGANAATAT

AATGTCTAAA

TGCTGCATGT

CC'ITCGGCA

AAAAGAGCAT

GCAAATTGAA

TGCATTGGTT

GCTCGTTCTT

AAAACCCCAA

AGAAGGCTTA

GAATGTAACA

TCCCACAGAT

TrTGAAAAAG

AGCAGATGCT

TGGCTTTAAG

CAAAAAAAGG

ACAAATCAGC

GCAGCTAGCT

CCAGATATTC

AAAGGCCTAA

GCAGAAAAAC

AITTCTGCCTA

ATGATAGCAA

AAAGCTTTAG

GAAGTCCTTG

GCAGCA.ACCA

CCAATTATCT

T=mAAATr

TTAACAAAGT

CTGTGTTCCT

CGCAAAAAGA

TAGAATCAGT

AGGTAACTGG

AAGCCGATGT

TAATTAATAA

ACCAAGATCA

TTGGTGATCC

GCGCTT'CTC

AATGGGTAGC

ATTCAAAAGT

ATCCAGTTKG

T'TTTACAGAG

AG

GCTTAGFFI'A

AACAGTTTCA

CATGGAGAAG

AACCTATGAT

AM'TCTCA

AAAAAGTGTC

AGCAAAGTGG

TGCAAGTGTT

TTATGTAGA-A

TAATGCAAGT

AGCGATTGTT

TAAAGTTGCC

TCAACAATGC

EFOSI-2 (SEQ ID NO:190) MSKQ KKAVFLLSLF SLVALIAACT N~QPQKETVST KKEEITLAAA ASLESVMEG( I IPAFEKEHP DIQVTGTYDS SGKLQMQIEK GLKADVFFSA STKQNNALVA EKLINKKSVV PLLENQLVLI VPNQDQAXWH DFSDLKKAQM IAIGDPASVP AGQYAEEG.LK ALGAWSYVEK HAS FGTNVTE VLEWVANASA EAGLVYATDA ATNSKVAIVA AMP EAVLKKP I IYPVGKVAA SKKQKSADAF LNFLQSQQCR KYFXNIGFKL TK EF051-3 (SEQ ID NO:191) ATOCT ACAAATCAGC CGCAAAAAGA AACAGTTTCA ACAAAAAAAG AAGAA.ATAAC CCTTGCGGCA GCAGCTAGCT TAGA.ATCAGT CATGGAGAAG 129 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faeczlis Genes.

AAAATTA'TTC

AGTTCTGG;A

GCTTCGACAA

GTTCCTTTAT

CATCATTTTT

CCAGCTGGTC

AAACACGCAA

GCAGAAGCTG

CC CATCC GC CTCTAAGA

AGAAAATATT

CAGCCTTTGA

AATTACAGAT

AACAAATCAA

TGGAAAACCA

CTGATT~TAA.A

AATATGCCGA

GCT=tGGCAC

GCTTAG=?A

CTCAACCTrCT

AACAAAAATC

T'TGANA.ATAT

AAAAGAGCAT

GCAAAT'IGAA

TGCATTGGTT

GCTCGTTCTT

AAAAGCCCAA

AGAAGGCTTA

GAATGTAACA

TGCGACAGAT

TTTGAAAAAG

AGCAGATOCT

TGGCTWAAG

CCAGATATTC

AAACGCCTAA

GCAGAAAAAC

ATTTGCCTA

ATGATAGCAA

AA.AGCTTTAr.

GAAGTCCTTG

GCAGCAACCA

CCAATI'ATCT

TTTTTAAA'T

T.TAACAAAGT

AGGTAACTGG

AAGCCGATGT

TAATTAATAA

ACCAAGATCA

'ITGGTGATCC

GCGCI'CGTC

AATGGGTAGC

ATTCAAAAGT

ATCCAGTTGG

TTTTACAGAG

AG

AACCTATGAT

ATTTTTCTCA

AAAAAGTGTC

AGCAAAGTGG

TGCAAGTGT

TTATCTAGALA

TAATGCAACT

AGCGATTGTT

TAAAGTTCC

TCAACAATC

EF051-4 (SEQ ID iNO:192) CT NQPQKETVST KKEEITLAAA ASLESVMEKK IIPAFEKEHP DIQVTGTYDS SGKLQMQIEK GLKADVFFSA 2ILLENQLVL I VPNQDQAI(WH DFSDLKKAQM IA IGDPASVP HASFGTNVTE VLEWVANASA EAGLVYATDA ATNSKVAIVA SKKQKSADAF LNFLQSQQCR KYFXNIGFKL TK EF052-1 (SEQ ID NO:193)

STKQMLALVA

AGQYATEEGLK

AMPEAVLKKP

ECKLINKKSVV

ALGAWSYVEK

I IYPVGKVAA

TAAAGTAGGA

CCCACA-ATGG

'ITACTTAGTT

NGAAATCTTC

GCAGAAAATA

TCAAAAAAAA

GTTCGTAGCA

CTAACCTGGT

GAAGCGCAAG

GTACCATGG

CGTCGTGGAG

NGTTT'ITAT

GGGAGACCAC

ATCCAGTI'GT

GAACGCAAAT

ETGGCATATT

CGAAAAAAGT

TACCATTATC

AGAAGTGGAA

ATTGTGGTT

AGAAGTCGGA

GAATGTATT'G

AAAAAGATTA

GTrTTrAATA

GAATCAATCG

TTTGACAGTA

AA.ACGAAGGG

CTATTAATT

ATCCGGTTTA

CCGCAA.ACAA

CCTAAAACTG

AGTAGTI=T

GCAGCGTATC

GCACTTCCTG

GAAGTGACAT

ATATTCCTCA

CAAAAACTTC

CCATTCAATC

GTGACAATCG

GGCTG=I1CT

AAGTAGTGAT

GXITGATTGC

AATGAGANGA

A.ACAACTTAT

AGACATACCA

GCTATCAATC

AATAACTTGG

ATIAGACAA

TTATGTAGAA AAGGAGAATA A EF052-2 (SEQ ID NO:194)

MRXX

NLXFLLLLVL LIYIPQTYA ENRETTEVGI GFTKTSDIPS KKNPWVNVLP QTTIQSLSIV RSRTQIKRLP KTGDNRITWL SWFGILFLIS SFWLFLFRQL CRKGE EFOS2-J (SEQ ID NO:19S) AGAAAATA GGGAGACCAC AGAAGTCGGA ATCGGCITA CAAc'I'rC ACACATACCA TCAAAAAAAA ATCCAGTTGT GAATGTATTG CCGCAAACAA CCATTCAATC GCTATCAATC GTCGTAGCA GAACGCAAAT AAAAAGAT EF052-4 (SEQ ID NO:196) EN'RETTEVG I GFTKTSDI PS KKNPVVNVLP QTTIQSLS IV RSRTQ IKR EF053-1 (SEQ ID NO4:197) TAGTCATGGC ACCATAACAA GGAGGAGAGA AGTGACATGA AAAAATACCT TTTcTTAGT 130 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

TGTTTTAG

GAAAATAGT(3

ACAGCGACTA

TCATCCGTCC

ACCACCAATC

TITATTAAAA

A'ITTAG

GTCTTTTCAG

GTGTI'GTCTC

GTAATTCTTC

TTTCTTCTGG

AAGCACCATT

GCAGAAGAGA

CTTCTGTCAT

CTTTTATGGA

CACAACGACC

CGTATATGGA

TATTTATITG

AGATCAAAAA

TCAGACACTG

ACGTATGAAT

GAACCCACCA

TCGCGACAAG

GGAATCACC

AACAGTATrA

CGT=I-GAGA

ATCCCACAGA

AGCCAGCTGA

GAAGATrACC

TTATCACTAT

GCAGTAGTAG

AGCAGCCTAT

AGAGTCGACA

CGGAGGCGCT

AGCGACAGGT

AGGCATATTA

GGATTGTAGG

0*

S

EF053-2 (SEQ ID NO:198) K4KKYLLLSC FLGLFSFCHS DTAFGEAAYE NSGVVSFYGT YEYPTEESITT ATSNSSTTTE PTKPADGGAS SVLSSGVYGS RQ-GRLJPATrT TNQAPFIYLG ISLITIGILF IKRRREDEKN SISSSROCRI EFOS3-3 (SEQ ID NO:199) TTTGGAGA AGCAGCTTAT GAAAATAGTG GTGTTGTCTC CTT=ATGGA ACGTATGAAT ATCCCACAGA AGAGTCGACA ACAGCGACTA CTAATTC-TTC CACAACCACC GAACCCACCA AGCCAGCTGA CGGAGGCGCT TCATCCGTCC TTTCTTCTGG CGTATATCGA TCGCGACAAC GAAGA EF053-4 (SEQ ID NO:200) FGEAAYE NSGVVSFYGT YEYPTEESTT ATSNSSTTTE PTKPADGGAS SVLSSGVYGS RQGR EF054-1 (SEQ ID NO:201)

TA.AATAAAAA

AGTGCAGTAC

ACAGATCCAG

ACTGAGCCGG

AAGCCAACCG

ATTGATCCTC

CCAACCGAGC

GAACCAACTC

GAACAGCCCA

AATCAATCGG

ACACATGTAC

GTACATAAAG

TATAAGGTTT

TTGCCACATA

ITAATTTCAG

ATTArrGGA

TAGTATI'CGG

CAACTCCACC

TGGATCCTIGC

AGCCTACAAC

CAACGCCGGT

CTACAACACC

AACCAGTGAT

CTAAACCAAT

CAGGAACACA

CAAGTGAAAC

GTTTCCT

TACCTACC

CAGGAGAGAA

CATTCTTrT

GGA.AATTACA

TGGCGGAAGT

AATTACCGAA

AGAACCGCCA

ACCGAGTGAG

TCAACCGACT

AAGCGAACCA

TCCAGAAAAA

AGACGTAGTC

ACCAAGTATT

TACTCCAAI'I

AACCAAAACA

AAACGTTGAG

ATTCACACTC

CTTTAAAAAG

ATGAAAAAAA

ATA.ACAGCAT

CCAACTGAT

GTAATACCAA

CCAGAAAAGC

GAACCAACCG

GAAAAACCTG

CCAGCAGAAC

GTTACACCTA

CCTATTGAAA

ACAACAGAAG

CCAGAAGGAT

GTAAAACCAA

CTTTTCTCTG

AATAAGAAA

TTATTIATC

TCGCTGACGA

CTAGTGAkACC

CTGATCCAAC

CAACAGAACC

AGCCAACAGA

TTACTCCAGA

CAGAAACACC

GTGGAGAAAT

CAAGCAACTT

CTGGGGAAGA

TAAAACCAAT

GTGATGGAAA

TATTGGGAAG

AAGCTTAA

PAGCTTT

TTTAGGACCA

TACGAATCCT

AGAACCAAGC

AACPLACGCCA

ACCTAGTCAA

ACAACCGAA

AAAA.ACTCCT

TGATAAAACG

AGCGGAGGTA

AAITGTAGCA

TAGCAGCTCG

AATGAAAGTA

CTCTTTGTA

EF054-2 (SEQ ID NO:202) M KKIILSSLFS AVLVFGCSI TAFADDLGPT DPATPPITEP TDSSP-PTNPT EPVDPAEPPV IPTDPTEPSK PTEPTrPSEP EKPTEPTTPI DPGTPVEPTE PSEPTEPSQP TEPTTPSEPE KPVTPEQPKE PTQPVIPE-KP AEPETPKTPE QPTKPIDVVV TPSGEIDKTN QSAGTQPSIP IETSNLAEVT HVPSETrPIT TEAGEEIVAV DKGVPLTKTP EGLKPISSSY KVLPSGNVEV KASDGKMKVL PHTCGEKFTLL FSVLCSFFVL ISGFFFFKKN KKKA EF054-3 (SEQ ID NO:203) 131 TABLE 1. Nucleotide and Amidno Acid Sequences of E. faecalis Genes.

A

ACAGATCCAG

ACTGAGCCGG

AACCCAACCG

ATTGATCCTG

CCAACCGAGC

CAACCAACTC

GAACAGCCCA

AATCAATCGG

ACACATGTAC

GTAGATAAAG

TATAAGGT'T

T

CAACTCCACC

TGGATCCTGC

AGCCTACAAC

GAACGCCGGT

CTACA.ACACC

AACCAGTGAT

CTAAACCAAT

CAGGA-ACACA

CAAGTGAAAC

GTGTTCCGTT

TACCTAGCGG

AATrACCGAA

AGAACCGCCA

ACCGAGTGAG

TCAACCGACT

AAGCGAACCA

TCCAGAAAAA

ACACCTACTC

ACCAAGTATT

TACTCCAATT

AACCAAAACA

AAACGT1'GAG

CCAACTGATT

GTAATACCAA

CCAGAAAAGC

GAAC CAAGCG-

GAAAAACCTG

CCAGCAGAAC

G'ITACACCTA

CCTATI'GAA.A

ACAACAGAAG

CdAAGGAT

GTAAAACCAA

CTAGTGAACC

CTGATCCAAC

CAACAGAACC

AGCCAACACA

TI'ACTCCAGA

CAGAAACACC

GTGGAGAAAT

CAAGCAACT

CTGGGGAAGA

TAAAACCAAT

GTGATGGAAA

TACGAATCCT

AGAACCAAGC

AACAACGCCA

ACCTAGTCAA

ACA.ACCGAAA

AAAAACTCCT

TGATAAAACG

AGCGGAGGTA

AATTGTAGCA

TAGCAGCTCG

AATGAAAGTA

a a.

a.

a.

a a a.

a a a a.

a a a EF054-4 (SEQ ID NO:204) DDLG-PT DPATPPITEP TDSSEPTN'PT EPVDPAEPPV IPTOPTEPSK PTEPTTPSEP EKPTEPI~rPI DPGTPVEPTE PSEPTEPSQP TEPTTPSEPE KPVTPEQPKE PTQPVIPEKP AEPETPKTPE QPTKPIDVVV TPSGEIDKTN QSAGTQPSIP IETSNUAEVT HVPSETTPIT TEAGEEIVAV DKGVPLTKTP EGLKPISSSY KVLPSGNVEV KASDGKMKV EF055-1 (SEQ ID NO:205) a

TAACAAAAGG

GGAGGTFTT

AGTT=TTA

TACCAAAATC

GCTGATCCCA

CAGACCACCA

AGCATTCTCN

CATGAAGCAA

TTGTI'TGTC TITCTGTGT CAATG-AAAAA AAAGCGTTAT TAAATGTTGA AGCGTCTGAT CGAGAACACC CGCTCCTAAA AGGAACCAGC TGGTCCTCCG CAACTGGCTC AACGCTCCCT GNTTCGCCTT AATCGGT=

ACTAA

AAAAGGGCAA

TTAATGAITG

GGTGGTTCTA

GATCCCCCAC

CAAGGACATC

GAAAGGCTAG

TGTGTCTACT

GTTCGGTGGG

CGAAAACAGA

AACGAAGTGG

CGAGTTAAAA

ATCTTCTCCT

GATTGAAITTT

TGCGCCAGCT

TGGTTCGACA

GGCAAATTTG

GAAGGGACGA

CGTACAGGGA GCAAGAGTCA GCGGGAATCG TACATAGAAA EF055-2 (SEQ ID NO:206) MrKKKRYL NIVCLLSSPS FFINVEASDG GSSSVGIEFY QNPRTPAPKD PPPKTDAPAA DPKEPAGPPQ GDQRSGGSTQ ILXFA.LIGLA GIVHRKGRH EAN TTTGSTLPR TGSKSQANLS EFOS5-3 (SEQ ID NO:2C7)

AGCGTCTGAT

TACCAAAATC

GCTGATCCCA

CAGACCACCA

GGTGG'rICTA

CGAGAACACC

AGGAACCAGC

CAACTGGCTC

GITCGGTGGG

CGCTCCTAAA

TGGTCCTCCG

AACG

GATTGAA TT

GATCCCCCAC

CAAGGAGATC

CGAAAACAGA TGCGCCAGCT AACGAAGTGG TGGTTCGACA EF055-4 (SEQ ID NO:208) SDG GSSSVGIEFY QNPRTPAPKD PPPKTDAPAA DPKEPAGPPQ CDQRSGGSTQ TTTTGST TABLE 1. Nucleotide and Amino Acid Seqeuences of E. fuecai-% Genes.

EF056-1 (SEQ ID NO:209)

TAAATGAAAA

ATAAATGTTG

CCGGCAACAC

AAGGAACCAG

ACAGCTGGCT

GGTCTTGTCT

AACTAA

AAAAGCGTTA

AAGCATCTGA

CCGCTCCTAA

CTGGTCCTCT

CGCAGCTCCC

TGATTGGTCT

TTTAATAATT

CGTGGTTCT

AGATGCCCCA

OCAAGGAGAT

TCGTACAGGA

TGTCGGAATG

GCGTGTTTAC

AGTTCGGTGG

CCGAAAACAG

CAACGAAGTG

AGCAAGAGTC

CTCCAGAGAA

TA=FTCCCC

GAA=C'A.ATT

ATGAGCCAC

GTGGTTCGAC

AGGCAAAkCCT

AGAAGGGACG

TAGI-PTTTT

TTACCAAA.AT

'TGCGGATCCC

ACAGACCACC

GAGCAI'CPI

ACATGAAGCA

EF056-2 (SEQ ID NO:210) MKXXRYLIIA CLLFSPSFFI NVE-ASEGGSS SVGIEFYQNP ATPAPKDAPP KTDEPAADPK EPAGPLQCDQ RSCCE=QTTT AGSQLPRTG-S KSQANLSILW LVLIGL/CMV QRKKGRHEA.N EF056-3 (SEQ ID NO:211) ATCTGA GGGTGGTTCT AGTTCGGTGG GAATTGAAT TTACCAAAAT CCGGCAACAC CCGCTCCTAA AGATGCCCCA CCGAAAACAG ATGAGCCAGC AAGGAACCAG CTGGTCCTCT GCAAGGAGAT CAACGAAGTG GTGGTTCGAC ACAGCTGGCT CGCAG TGCGGATC CC

ACAGACCACC

EFPO%-4 (SEQ ID NO:212) SEGGSS SVGIEFYQNP ATPAPKDAPP KTIDEPA-ADPK EPAGPLQGDQ RSGGSTQTIT ACSQ EF057-1. (SEQ ID NO:213)

TAATCTTTAT

ATCATAAAAA

AGCGNTATGG

TACGTI'TATG

CAGGGAAGAA

GAAAATCCGC

AATAAAATGA

TGGCTGGCC

GGTTTAGTTT

CTGAAGAAAA

AGAATGAAAA

ACAATTTAGC

TGTATTCCTT

AACGAZAGAA

AGTCAATGTT

GGTATGTTTA

TAATTATGAA

AGAGTCAAAT

TGCTTCTGGA

GATAGGAGTT

GAAAATGGGC

GGGCTATTA

TCAAATGGTC

GACGTAGCCT

CAAGCAGT'I

AGTTTG

A.AGGAGGAAT

TCATTGGTT

AAGCGACT

ATACCCAACA

TACCTAAAAC

GGATAGTCAT

TCAGATCAA

GCNAACAAAA

CTATGGTACC

ATCAGAAGAA

AGGCGAGTCT

TTAT=AAT

GGAC TTATT TALA EF057-2 (SEQ ID NO:214) MXI IKRFSLVCLG LLIIGLXTKS XMAEENNYES NGQASFYGTY VYENEKESND VAYTQQSEEQ GRNNLAASGQ AVLPKTGESE NPLYSLIGVS LLGIVIYLI"

KMKREKEFI

EF057-3 (SEQ ID NO:215) AAA TAMTTATGAA TCAAATCCTC AAGCGACCTT CTATGGTACC TACG ITTATG AGAATGAA.AA AGAGTCAAAT GACCGTAGCGT ATACCCAACA ATCAGPACAA CACGGAAGAA ACAATTTAGC TGCTTCTCGA CAACCAGTTT EF057-4 (SEQ ID NO:216) EENNYES NGQASFYGTY VYENEKESND VAYTQQSEEQ GRNNLAASGQ AV 133 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. foecalis Genes- EF058-1 (SEQ ID NO:217)

C.

C C

C.

C C

C.

C C

C

C

C

CCC.

C

TGAAGAACGT

TITGGCTATTT

TGGTACACCG

ACAACTGCAT

ATGACGGAT

AAATATCAAG

GAGCAACGAG

CCTGGGAAAC

CCTAAAAAAC

GTAGTTGCTG

GATGG'-!TCCT

GTGGTAGCCA

TTAAATGGCG

ATCCAAGCAG

=F~ATTACTG

ACGGGAGAAT

GCTCCAAATA

AACAATCAAA

ACACCAAGCT

GTAAATATTC

AATTTAGTTG

GCTTATGCGT

CAAGCAAATG

GGCACACTAA

'ITTAAAAATG

GAAGTTGTGA

GCCTTGGCGG

AAAATCGATG

rI'TACAACAA

TTAGAAGAA-A

GTCAATGA-AC

AAACACAA.AG

GGCCCTCT

TAA

TCTA'TT-GC-T

TATCAAAA-AC

TTAGTACCTT

TTGCAGAAGA

TACCAGATCC

GACTGGCAGA

CGGCAGGCGC

CTMTTGCTCA

AAAATGGTAA

CTACGAATAT

ATAAATATGG

ATGATGGTAG

CAGAATTTGT

TCAAAGATG

GGAAAAGTTA

TAACAGTTAA

ATGCAGAAT

CACCTGTTGA

TAGATGGTAA

CATTGGGGAT

ATAAACATGA

TATATGATGG

GCTTCACTGT

AkATTCGTTA

AGCGAATGT

CAGGTGGGAA

GAGCTTCCTT

AAACAACGAA

CGGCTGATGG

CTGTAGCTCC

AATCATATGG

GTACCTTACC

TGCTACTTAT

TGACGATTGC

AAAAAAGAGG

GTTACTAAT

AAATGGGGAG

GCCTATCAA.

TGTGACGTT*T

AAGCGTTGAT

AGGAACCACC

AGATGCAGTC

GGTGGTGGCG

AACAGAAGAA

TT-TACATGTG

TATTTCTAAA

ATTATATACA

TGAAATTGGC

AAATCTI'GAG

AA ITCAAAAT

AAAAACAGTC

AGATGTGGCA

TGCAGACAAA

TGCAGCCTTA

GGATACAGTG

CGCCGTTAAT

CTTTATIGCAT

TGATAACGGT

ACGTTTCATT

TGTCCTCCGT

AGCAGCAACT

ATTAGTTGAT

TCATGATTAT

CACAACAGAA

TTCAACAGGT

TGCAGC-AGTC

AGGCCTGCTA

AGAGAGAAAA

TTGCCAC~rT

PGCGCACAGC

AATAGCGGGA

A.GTATTTATA

GCAGCTAAAC

6ATGCAAATG

TATACCATA

TTCCCAGT

TTAGCGGTTG

AAAAAAGTAG

AGCGAAGGCT

TGGACAACGG

GAAAATGATT

GTTGGTTCGT

CAAACAAAAA

AAA.AATCATA

ATTGGCGAAA

GAAGGCGACG

ACTT=TATA

AT1'CTCCTG

CCAGCGTATA

TTAAATGAAA

CATACCGACG

AAAGTCGATG

GATCAAAACA

TGGGTGAAAA

ATCACAGGGC

CTCTTG ITAA

AACCTAGTTT

GGCAAAGGAA

TACTTTGCTA

A.TCATTGGGA

TGAAGCAA T rcACAAGTGT

I'CGTGATTCA

AAGAAATCAG

A.CGTGACGAA

AAGCTGTCCA

GGAATGTCAC

AAGAAGAACC

ACGAAATGAT

'ITCATAMTA

GAACTGCTGA

CAC CAGGCAC

ATA-AAGAACA

TCA.CAGAAGC

ATA'F"IAGA

CAC CATTTAC

CCTCTAA.AGT

AAATrAAATA

CTAATAAATA

ACGTGACTTC

AAAATTATCA

ITrCCTACGCT

AAGCAGATCC

ACCAAACACC

GCGATGTGAC

GCGACACAGC

CAA.AAGCTGA

TTAAATACGG

CAA.ATCGGAT

CACCACAAAA

TCTACGTTA

GACGTAGAAA

E'GGTAGTCAT

kAAAAAAGTT

%.TTAGGGACA

CAAAAAGAAA

CACTTTGAT

CGAATTTTAC

AAGTTTAACT

TGTTCAGTTA

PAAAGAGGGT

CAAGCAAACA

TCCTAAAAAT

AAATGAAGGA

AGTAAAATAT

AGCAAAACC

AGAGAATGGA

AGA.AGTAAA-A

AATTGAAGCA

TGATAAAACA

TCAAATTTCT

CGTCAAATTC

TGGAGAGTAT

AGTGACTGAA

AACACCAGGC

TACGAAAGGC

ACCAACTGTT

AGCGACACAA

AAATTA'ITTG

AGCAACTACT

TACCTATTAT

TCAATTTGTG

AGTACCAAAC

CTTAGGAAGT

AGAAAATGCT

EF058-2 (SEQ ID NO:218) 14KQLKKVW YIVSTLLLIL PLFTSVLG TT

TAFAEENGES

QRAAGASVDA

VAATNMVVAF

NGAEFVISKS

GELTVKNLEV

PSLDGKDVAI

YALYDGDTVhI

KNEANVDNCH

IDETTKAATW

REQSYGTTEN

AQLVIHKKKM

AKQAVQSLTP

PVYEMIKQTD

EGSPGTVKYI

GSYILEEVKA

GEKIKYQISV

APENYQVTEQ

TDDQTPPTVE

vVTKAEATTF LVS PEKVPN( TOLPDPL IQN

GKPVAQGTTD

GSYKYGTEEL

QGVKDGLYW

PNNAEL IENQ

NIPLGIADKE

ANGFTVAVNP

WVTGGKRFIK

'ITTADGLVDI

HKGTLPSTGG

SGKEMSEFDK

ANCNVTVIQLP

AWN IYPKNV

TTDKEQAKRF

TKTPFTIEAN

GDANKYVKFN

AYIPTLTPGG

VDGDVTATQA

TGLKYGTYYL

KGIYVYLGSG

YQGLADVTFS

KKQNGKDAVY

VANIDGSLHVK

ITGKSYEIGE

NQTPVEKTVI(

LVOKHDAALT

TLKFVYFMHL

LAGASFVVRD

EETVAPDDYV

AVLLLIAGVY

IYNVTNEFYE

TIKEEPKEGV

KvGTAENEGL

NDFTEA.ENGT

NDTSKVDKTT

FDN~VTSGEYA

NEyKADPTKGF

QNSDTANYLK

LLTNRI EFVV

FARRRKENA

EF058-3 (SEQ ID NO:219) 134 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecaiis Genes.

ACAAGA AAATGGGGAG AGCGCACAGC TCGTGATI'CA CAAAAAGAAA

ATGACGGATT

AAATATCAAG

GAGCAACGAG

CCTGGGAAAC

CCTAAAAA.AC

GTAGTTGCTG

GATGGTTCCT

GTGGTAGCCA

TTAAATCGCG

ATCCAAGGAG

TTTATTACTG

ACGGGAGAAT

GCTCCAA.ATA

AACAATCAAA

ACACCAAGCT

GTAAATATTC

AA'ITTAGTTG

GCTTATGCGT

CAAGCAAATG

GGCACACTAA

TTTAAAAATG

GAAGTTGTGA

GCCTTGGCGG

AAAATCGATG

TTTACAACAA

TTAGAAGAAA

GTCAATGAAC

AAACACAAAG

TACCAGATCC

GACTGGCAGA

CGGCAGCCC

CTGTTGCTCA

AAAATGGTAA

CTACGAATAT

ATAAATATGG

ATGATGGTAG

CAGAATTTGT

TCAAAGATGG

GGAAAAGTTA

TAACAGTTAA

ATGCAGAATT

CACCTGTI'GA

TAGATGGTAA

CATTGGGGAT

ATAAACATGA

TATATGATGG

CTCACTGT

AATTCGTT-TA

AGGCGAATGT

CAGCTGGGAA

GAGCTTCCTT

AAACAACGAA

CGGCTGATGG

CTGTAGCTCC

AATCATATGG

GTACCTTACC

GCTTATTCA.A

TGTGACGTI'

AAGCGTTGAT

AGGA.ACCACC

AGATGCAGTG

GCGTGGCG

AACAGAAGAA

TTACATGTG

TATTTCTAAA

ATTATATACA

TGAAkATTGGC

AAATCTTGAG

AATITCAAAAT

AAAAACAGTC

AGATGTGGCA

TGOCAGACAA

TGCAGCCTTA

GGATACAGTG

CGCCGTTAAT

CTTTATGCAT

TGATAACGGT

ACGTTCATT

TTCGTCCGT

AGCAGCAACT

AFI'AGTTGAT

TGAMALAT

AATACGA

AGTATI'TATA

GCAGCTAAAC

GATGCAAATG

TATACCA2TA

TTCCCAGTTT

TTAGCGGTTG

AAAAAAGTAG

A.GCGAAGGCT

TGCACAACGG

GAAAATGAr

GTTGGTTCGT

CA-AACAAAAA

AAAA-ATGATA

ATTGGCGAAA

GAAGGCGACG

ACTTTTGATA

A'TTGCTCCTG

CCAGCGTATA

TTAAATGAAA

CATACCGACG

AAAGTCGATG

GATCAAAACA

TGGGTGAAA.A

ATCACAGGC

GTCTTGTrA.A

AAGAAATGAG

ACGTGACGAA

A.AGCTGTCCA

GGA.ATCTCAC

AAGAAGAACC

ACGAAATGAT

TTCATAITT

GAACTIOCTGA

CACCAGGCAC

ATA.AAGAACA

TCACAGAAGC

ATAT=~AGA

CAC CA=FAC

CCTCTAA.AGT

AAATTAAATA

CTAATAAATA

ACGACTTC

AAAATTATCA

TrCCTrACGCT

AAGCAGATCC

ACCAAACACC

GCGATGTGAC

GCGACACAC

CAA.AAGCTGA

TTAAATACGG

CAAATCGGAT

CGAGTI'GAT

CGAATTTTAC

AAGTTTAACT

TGTTCAGITA

AAA.AGAGGGT

CAAGCAAACA

TCCTAAAAAT

AAATGAAGGA

AGTAAAATAT

AGCAAAACC

AGAGAATGGA

AGAAGTAAAA

AATTGAAGCA

TGATAAAACA

TCAAATTTCT

CGTCAAATTC

TGGAGAGTAT

AGTGACTGAA

AACACCAGGC

TACCAAAGCC

ACCAACTGTT

AGCGACACAA

AAATTATTTG

AGCAACTACT

TACCTATTAT

TGAATTTGTG

AGTACCAAAC CACAACAGAA AACCTAG7TT CACCAGAAAA EF058-4 (SEQ ID NO:220) EENGES AQLVIHKXXN TDLPDPLIQN SGKEMSEFDK YQCLADVTFS IYNVTNEFYE

QRAAGASVDA

VAATNMVVAF

NGAEFVISKS

GELTVKNLEV

PSLDGKDVAI

YALYDGDTVI

KNEANVDNGH

IDETTKAATW

NEQSYGTTEN

AKQAVQSLTP

PVYEMIKQTD

EGSPGTVKY I

GSYILEEVKA

GEKII(YQISV

APENYQVTEQ

TDDQTPPTVE

VKTKAE-ATTF

LVSPEKVPNK

GKPVAQGTTO

GSYKYGTEEL

QCVKZDGEJYTW

PNNAEL IENQ

NIPL.CIADKE

ANGFTVAVNP

VVTCGCKRFIK

TITADGLVDI

HKGT

ANGNVTVQLP

AVVI{IYPKUV

TTDKEQAICRF

TKTPFTIEAkN

GDANKYVKFN

AYIPTLTPGG

VDGDVTATQA

TGLKYGTYYL

KKQNGKDAVY

VANDGSLHVK

ITGKSYEIGE

NQTPVEKTVK

LVDXHDAALT

TLKFVYFMHL

LAGASFVNRD

EETVAPDDYV

TIKEEPKEGV

KVGTAENEGL

NDFTEAENGT

NDTSKVDKTT

FDNVTSG'EYA

NEKADPTKGF

QNSDTANYL<

LLTNRIEFVV

EF059-1 (SEQ ID NO:221)

TAGATTGGAA

TTAGCAGCG

ACAACAGGGA

GAGCCAGAGC

ACCGAACCTA

CCAACAGAC

GTACCAGAGC

GAATGAAAAT

GAAGCAGTGT

GTT=ACC

AACCAACAGA

GTGAGCCTTC

CAACAACGCC

AACCAACAGA

GAAAAAAATC

TTCTGCTTAT

AGATCAACCG

GCCAAGTACA

AAAACCGACG

AAGTAAGCCA

GCCAAGTGTA

ATTATTATTG

GCGCAAGAAT

AATGTACCAA

CCAGAGCAAC

GATCCTI'CGT

GAGCAACCAA

CCAGAAAAAC

CCTTATTCAG

CAGAAGGAAA

CTGACCCAAT

CATCGGAACC

TACCAGACGA

CAGAGCCAAC

CAGTAGAACC

TACAACCCTT

TCTTGGTGAA

AACGccAAGT

CTCA.ACACCA

ACCGAGCGTA

AACGCCAAGT

AAATAA.ACCA

135 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. fiaecalis Genes.

ACCGAGCCAG

GAGCAACCAA

CAACAGCCAA

GTGACGACAC

CCTTAACAC

AGTGGCAATG

GAAAAAAG

TTAATTTATA

AAAAGCCTGT

CAGATGTGGT

CTGTTCCAAT

CGATTGAAAC

AA-ACGCCTGA

TACAAGTGAA

GCATAATTGG

AAAAACGTAA

GCCAGTTGTT

GGTAAAGCCA

TGAAACGAAT

AGCAAGCGGA

TGGATTAAAA

AAGTGCTGAC

GTCAATCGCT

AGTCTAG

CCTGAAAAAC

AATGGAGAAA

AACCT=CAG

GAAGCAATrG

CCGATTAAAA

GGAAAAATGA

GGTGTATGTT

CAGrI'GTACC

TTGCAACAGG

AkAGTAACACA

TCGCAGTGGA

GTGAATATAA

AAGTACTTCC

TGACTGT'PT

ACAACAACCA

AGAATCTACA

TGTCCCAACT

TAAGGGCGT

AGTATI'ACCA

TTACACTGGT

ATCAGGAATC

EFOS9-2 (SEQ ID NO:222) MKKNI IIALFSTSLL AGCSSVSAYA QESEGNLGET- TCSVLPDEPN VPTDPITPSE PEQPTEPSTP EQPSEPSTPT EPSEPSKPTD PSLPDEPSVP TEPTTrPSKPE QPTEPTTPSV PEQPTEPSVP EKPVEPNKPT EPEKPVPVVP EICPVVPQQPE QPTDVVVKPN GEIATGESTQ QPTVPIETNN LSEVTHVPTV TTPIETASGE AIVAVDKGVP LTQTADGLKP IKSEYKVLPS GNVQVKSADG KMKVLPYTGE KMyGIIGSIAG VCLTVLSGIL IYKKRKV

C

C.

C.

C

b*

C*

C C

CS

C.

C C

C

C

C

S

C

EF059-3 (SEQ ID NO:223) AGAAGGAAA TCTI'GGTGAA

ACAACAGGGA

GAGCCAGAGC

ACCGAACCTA

CCAACAGAGC

GTACCAGAGC

ACCGAGCCAG

GAGCAACCAA

CAACAGCCAA

GTGACGACAC

CCT'ITAACAC

AGTGGCAATG

GMI TTTACC

AACCAACAGA

GTGAGCCTI'C

CA.ACAACGCC

A.ACCAACAGA

AAAAGCCTGT

CAGATGTGGT

CTGTTCCAAT

CGATTGAAkAC

AAACGGCTGA

TACAAGTGAA

AGATGAACCG

GCCAAGTACA

AAAACCGACG

AAGTAAGCCA

GCCAAGTGTA

GCCAGTTGTT

GGTAAAGCCA

TGAA.ACGAAT

AGCAAGCGGA

TGGATTAAAA

AAGTGCTGAC

AATGTACCAA

CCAGAGCAAC

GATCCTI'CGT

GAGCAACCAA

CCAGAAAAAC

CCTGAAAAAC

AATGGAGAAA

AACCTTI'CAC

GAAGCAATTG

CCGATTAAAA

GGAAAAATGA

CTGACCCAAT

CATCGGAACC

TACCAGACGA

CAGAGCCAAC

CAGTAGA.ACC

CAGTTGTACC

TTGCAACAGG

AAGTAACACA

TCGCAGTGGA

GTGAATATA.A

AAGTAC

AACGCCAAGT

GTCAACACCA

ACCGAGCGTA

AACGCCAAGT

AAATAAACCA

ACAACAACCA

AGAATCTACA

TGTCCCA.ACT

TAAGGGCGTT

AGTATTACCA

EF059-4 (SEQ ID NO:224)

C

0C C 0*

C.

EGNLGET TGSVLPDEPN VPTDPITPSE PEQPTIEPSTP EQPSEPSTPT EPSEPSKPTD PEQPTEPSVP EKPVEPNKPT EPEKPVPVVP QPTVPIETNN LSEVTHVPTV ITPIETASGE GNVQVKSADG KMKV PS LPDEP SVP

EKPVVPQQPE

A IVAVrDKGVP

TEPTTPSKPE

QPTDVVVKPN

LTQTADGLJ(P

QPTEPTTPSV

GEIATGESTQ

IKSEYKVLPS

EF060-1 (SEQ ID NO:225)

TCAAAAATAG

ACGTTGGGAA

GCAGAAGAAG

AAAACGGAAG

TCACAACAAC

CAGACGTCAT

GCCTGTITAC

AAATAA

ACAAGGAGCA

TACTr'IAAC

TAGGGCAAAC

GTGTCAITAA

AAGACAAAGT

TACCAAGGAC

TCGTAGTACT

CGCGATGATG

CGTTGGCATT

GAATATCGGT

GCCAATAGAG

GAGCAGAAAA

AGGTGAACGA

AACAAGTTTC

ACAATGAAAA

GCGAGTCTAA

GTAACGT=C

CAACCAGTCA

ACCACTGCTA

AATAGCACGT

TATATTTGA

GTAAAGGGTC

TTGTTCTC

ATGGAGGAAA

CTGATAAAGA

AAACGAATCC

GGCTTTACAG

ATAAAAAAAG

ACTI'CTGGTG

TGAGAGTTTT

AGAGCCACTA

TAAAAAAACG

GACTAATGCA

CCTTGGTATT

GAAAAAGGAA

EF060-2 (SEQ ID NO:226) MMT MKSKGSLLVT LGILLTVGIA SLIVSSIESFA EEVGQTNIGV TFYGGKEPLK 136 TABLE 1. Nucleotide and Amino Acid Seqcuences of E. faecalis Genes.

'rEGVIKPIEQ PVTDKDKKTS QQQDKVSRKT TAKTNPTNAQ TSLPRTGERN STWLYSLGIA CLIJVVLTSFY YLNKXRKXXEX EF060-3 (SEQ ID NO:227) ACAAGAAG TACCGCAA-AC GAATATCGGT GTAACGTTCT ATGGAGGAAA AGAGCCACTA AAAACGGAAG GTGTCATTAA GCCAATACAG CAACCAGTCA CTGATAAAGA TAAAAAAACG TCACAACAAC AAGACAAAGT GAGCAGAAAA ACCACTGCTA A.AACGAATCC GACTAATGCA

CAGACGTCAT

EF060-4 (SEQ ID NO:228) EEVCQTNIGV TFYGGXEPLK TEGVIKPIEQ PVTDKDKKTS QQQDKVSRKT TA-KTNPTNAQ TS EF061-1. (SEQ ID NO:229) 00 0

SS

SS

0 0 60

S*

S 0 00 0* 0O 0 600 S

S.

S S 000 S

TAATGGAACG

ATAATGATGA

AGTQAAATTT

GAAGTACCAA

CCACCTGTAG

CCGACAACAC

GAGCCAAGTA

GAAAAAACTC

CCAAGCAAGC

GGTACACAAC

CCTAGTGTAA

GGTCTTCCAC

TTGCCTAGCG

ACAGGTGAAG

ACCGACAGAA

AAAAAATI'CT

CTGCTTTTC

CAGAACCAAG

ACCCTGTAGA

CAACAGAACC

AACCAGTAGA

TGACACCAAC

CAATCGACGT

AGCCAACAGT

CAACACCTAT

TTACACAAAC

GAAATGTAGA

AAATGAATAT

GAAGATTT1'G

TTTTGCTAGT

ACAAGAAATT

TACACCAGAA

GCCACCTATT

TACAACTCCT

ACCTGAAAAA

TAAACCAACA

TGTTGTAACG

CCCTATTGA-A

TACAACTACA

AGCAGAAGGG

AGTAAAAGGT

CTT=IATCT

AACTTACAAA

TTATTTAGTC

ATCCCTGATr.

AAGCCAACAG

ACACCA.ACGG

ACAGACCCAA

CCAGTTACAC

GAATCTGAAA

CCAACAGCG

ACAAGTAAT1'

GACGGAGAA.A

TTAAAACCTA

AAGGACGGTA

GCCGTAGCGG

TAAAATTAA AATGGAGGAA CCACACTACT ATTTGGGGGA ATACTACGAC ACCGCCCATT ATCCAACACC GCCAATTGAG AGCCAACAGA ACCGACAGAG GTGAACCAGA ACAACCAACG CAAGCAAACC AGCAGAACCC AACCAGTACA ACCAGCAGA.A AATTAAATCA CCCTCGAAAT TGGCAGAAAT CACGCACGTG ACATTTAGC TGTAGAAAAA TTCAATCNAG TTACAAACTA AAATGAAGGT TTTACCATAC TATCTTGTCT GTAG EF061-2 (SEQ ID NO:230) 0 'See

S

00 S0 MMX I LFASL

VPTEPSTPEK

PSKPVEPEKP

TQQPTVPIET

PSGNVEVKGK

FSATLLFGGS

PTDPTPPIEP

VTPSKPAEPE

SNLAEITHVP

DCKMKVLPYT

EISAFAQEII

PVDPVEPPIT

KTVTPTKPTE

SVTTPITD

GEEMNIFLSA

PDD'TPPIE

PTEPTEPTEP

SE:KPVQPAEP

GENIVAVEXG

VAVSCL

TTPTEP'ITPT

SKP IDVVVTP

VPLTQTAEGL

EPSEPEQPTE

TGELNHAGNG

KPIQSSYKVL

EF061-3 (SEQ ID NO:231) GAAA=T CTGCTTlITGC ACAAGAAATI' ATCCCTGATG ATACTACGAC ACCGCCCATT

GAAGTACCAA

CCACCTGTAG

CCGACAACAC

GAGCCAAGTA

GAAAAAACTG

CCAAGCAAGC

GGTACACAAC

CCTAGTGTAA

GGTCTTCCAC

TTGCCTAGCG

CAGAACCAAG

ACCCTGTAGA

CAACAGAACC

AACCAGTAGA

TGACACCAAC

CAATCGACGT1 AGcCAACAGT

CAACACCTAT

'ITACACAAAC

GAAATGTAGA

TACACCAGAA

GCCACCTATT

TACAACTCCT

ACCTGAAAAA

TAAACCAACA

TGTMGTAACG

CCCTATTGAA

TACAACTACA

AGCAGAAGGG

AGTAAAAGGT

AAGCCA.ACAG

ACACCAACCC

ACAGAGCCAA

CCAGTTACAC

GAATCTGAAA

CCAACAGGGG

ACAAGTAATT

GACGGAGAAA

TTAAAACCTA

AAGGACGGTA

ATCCAACACC

AGCCAACAGA

GTGAACCAGA

CAAGCAAACC

AACCAGTACA

AATTAXATCA

TGGCAGAAAT

ACATTGTAGC

TTCAATCiqAG AAArGAAGGT

GCCAATTGAG

ACCGACAGAG

ACAACCAACG

AGCAGAACCC

ACCAGCACAA

CGCTGGAAAT

CACGCACGTG

TGTAGAAAAA

TTACAAACTA

TT

137 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. facca lis Genes.

EF061-4 (SEQ ID NO:232) QEII PDDTTTPPIE VPTEPSTPEI( PTDPTPPIEP PVDPVEPPIT PTEPTEPTEP TTPT-EP'ITPT EPSEPEQPTE PSKPVEPEKP VTPSKPAEPE KTVTPTKPTE SEXPVQPAEP SK(PIDVVVTP TGELNHAGNG TQQPT'JPIET SNLAEITHVP SVTTPITTTI GENIVAVEKG VLTQTAEGL KPIQSSYKVL PSGNVEVXGK DGIQM(V EF062-. (SEQ ID NO:233) TGATTCTTGA AGCAACAAAT GAAAGCAAAA 0*

CACTGGGTAA

GATAATGTAC

AACCCCGACC

GTACAAAAAG

GGTACTGAAC

GGAGCTGAAA

CTAGGGCACC

CCTAAAGAAT

A.ACATTACCG

GCTGCAAAAC

CAAGCAGCTG

GCAAAAA.ACA

AAGAACGAAA

GTCGTGACGA

GAT=AATA

ATGGGNGGGA

TCTAATGGTG

GGACTAA.ACG

CAATCCTCAC

ACNGCTITA

AAGT-TC LrG

GCGCTGTCTT

GATTTTGGGG

GATGGAAAAT

AkATAGTGATT

GCNAATGGNC

GTATCTAGTG

ATTTCAATT

AAAGCCAATG

NTCAATGATT

ACAGAACTTG

GATGCAGAAA

CTNAACGANA

GTGACTGCTT

AACGCNGACG

GTAGTCAAAA

GAAACGGTAA

AAAGCAGATA

CAAACAAAA

ACNGAAGAAT

TGGCACGCTA

GATATTTCTC

AATCAAGCAT

CTGTCCCTAT

AAGCCGCGGA

TGCAGTCAGA

ACACTACI'C

AAAGTTCAC

AATCAGCACA

CAACAGAAGT

ITGAAACACC

TTGTTAAA

AAAAAGAAGT

AAT'rAAAAGC

AAGCNGAAAA

ACAGCTATGT

AAGACACTAA

AAGTAAATGC

AAGNTACTGG

GGTATr.CCOT

CTAGTTAT

CAAGCCAAAG

TTGGTACAAA

ATGCGTCACG

C ITTAAATTC

CNAACAATGC

TITACTCACC

GGC-ACGCTGT

CTATTTICCTT

CGCAATCGTT

ATGGGAATCC

TCGTTCCTGT

TAAATGTOAA

CCAAAGTAGA

AAGTGACGAT

AAGACTNAAA

CTTATGANCT

GNTCNGTTGT

ATGTAGAAGG

CAAATACAGT

AAAACGGTAC

TTTATTATGA

GGGGCATrGAA

TTACNAANTA

CCTACATTCT

TAITTGCNGC

TCTTTTTCTA

ATTAGATACG

AAAGGAAACA

TCAACCGACC

TACCCCAAAT

AGAACAACCA

TCCACCAGCT

AGACGTTGAT

ACCAGCAGAA

AGACCAACTA

CAAAAATGAA

AGAGCGNTAN

CAATGAAGCG

AATTCGTCG

AGGGGATTCA

CAACTTCCAG

TCTTIIAGAA

AGGACGTAAA

TGGAACGTTA

CAGAGTCAAT

TAA.AGAAGTA

AAGTTTAACG

GTTCAAATAC

GGAAGATATI'

AGGTCACAAG

TTCTI'TTGGT

TCCCTTTAGN

AAAAGAACCA

NCTTGTGCCN

NCGTGCCAT

TCCAAAAACA

TGATTTATCC

AGCTGTTGCT

CAATTTAGAT

TTTAGCAATG

CGATTTTGAA

GATTAACCAT

AGTTGGCAGT

AGTGAAATCT

TGATGTCTTG

TGACCTTAAA

TrTAGAAAAC

NTTAAATGAA

AAACAATATA

P ClrGTGTTAG

CAACCAGAAA

CCTAAAACGG

A~AAGTAGAAC

.ATACCACAA

GTAOTAAGCC

GAAA6ATGAAG

AAAGCAGTIG

GACTTAGGCA

CAAAAAGAAC

AAAATTGCCA

GANAPAAGAAG

AT1'AGTAAAA

ATTAAAGGCG

AA.AGATATCT

AA=TCTTTG

AAAAATAAAC

ATTACAAAAG

AATKGCAGTAT

GGTAACGATG

CTACCAGATA

AATAAAGGTG

ATTAATIGGNT

GACTATGGCA

AATGCC TACT

ATGACAACA.A

ACTAACTTAA

GAAAAAGCAA

AATAAAGAAG

TCTT'TACAAT

GTAACNAAAC

AAACTGAAAG

GCACNATTA

CAAAACACCG

GGGTATAAAT

AATACAG-CTG

GTGCCAGGTA

GTTTCTCTAC

TCCGAACGTC

GACACCACCC

GTAGGGGANA

AAAGACAATA

GGAAGcAATA

AGACATATAA

GAGCCGTAG

CAACGACGGT

CAGT~kTCTGA

AACTAGCGCC

ACGCGCAACA

CTGAAACAAC

TGA.ATAAATC

ATGAAGTAAA

ACG=ICTI'C

kAGCGAAAAA

AAGAAAATGC

TCGCNGAATA

ACCTAGTGTT

GAAAATTTAT

TTACAAAATT

AGCTAAGAkAT

AI'ACCTACT

TCAACCCAAT

AGAAGCAACA

AGAAAATAAA

ACCAACAGTA

CAATGAACCT

AACGTCCATT

AAAAGATCCA

TAAAGA=tTA

GATTGCCCAA

AGAAATTGCG

CAACAAGCAT

CGATCAATCT

CAAAGCAACT

ACGGAAAGAT

TAAAAGACGC AAATCTTGGG CACTCACAGT GACCTATACA CAGAATTTGT TTATGAACTA ITCAAACGA TCCGATATC ?I'AAAACACG CTTAACCA'Fr A.AGATAGTCC ATTTGCGTAT GCCATGCGGA ATTTGTTTCT CNTATGTGAA AAAACAAGCG CAGCACCTTC TGGATTGAA-A TTGGTCAGG TGTAGGTCTA AAGGAAAAAG TAATGTGCCT ATGCGCAATC AGTCAAGCCT CGATTGAAT CAATNGATAC TCACUGATGG NCAGAAAAAT ACATTCTGAC AG-GOGATACC AAGGGATTCG AGATACNTT 'IIATCAAGC AGACGCAAGT ATTCAGGAAN AGCTAAAGAC TCACAGCAAT CATCAAAACC ATTAC'ITGT CTTGCCG=r IrrcAGcTGAC AAANGATGGN GTAATCCTI'C CAAAGATGTA ATGATAAAGA TATTCCGTTA CAGCNAACTA TGGCGGAATN ATGATCGTIT cACAGGNAAA AAACGTTAAA AGCAGGAACA A-AGAC?M'AC GTTTFACNATG A.AGTAGGCAA ACAAGcTI'GG 138 TABLE 1. Nucleotide and Amino Acid Seqcuences of E. faecalis Genes.

TCTGTGTATC

AACTACAACA

AAACCAACCA

CGTGGTGATG

GCCTTTGATA

AANGTGACAC

AACCAGTTCA

CCACAAGCCT

GTCAAAGCCG

CGAATTAAAA

CTTATTAAAG

TTCTTCTATG

TGGTCGATTA

TI'GCCAATT

TCGAAACTAT

TTTI'TNGATG

ATTGGTGTAG

AATGAGAAGA

CCAGAAAAAA

TTAGTGGTAG

GTCTTATTAA

AAACGTAGAA

TGGAAGTCGA ACGGATNAAA AAGAGCTTCT NCG'ITCTAAT AAGCCG=TCA TAACAAGAAA TTCTTTCTTA TGAAATGACN

CACTCGATCT

CAATCAAAGA

CGATCTfCNTG

TTATTCTAGC

ATGT ETCTGG

CCAATACNGT

TNGGTGACAA

AATTTACAAG

GCGATAAACT

CTAA'TTTGT

TCACGATGAC

CGATGAATCT

AACGAATTGC

TTAAAACNAA

CAGTC-ATTGT

AAAAGGCAAG

CGGTAGCTGG

TGCGACACGC

CTTACTTCGT

GGACGATGCC

GNATGGTGGC

NGATGTI'AT

TOTCAACCAT

ACAAAGTCAA

TAGTGACATPI

AGACGTCAAA

TTAGCAGAC

CITTGAACAA

AAAAGAAAAC

GGCAGGAGAC

TACGGTAGTrC

ACCACCAACA

TGTNGTGCCA

TAGTTTAGCC

ACAGGTGACG

ACNCTGGTr.A

GGGGAAGANA

TGGGACTTAA

GTTTCTTTCT

GTCAAAGA'TT

AAAGGCACGG

CAAGAATTGC

AATTCAGCGG

ATTCCAAAAG

AATGGNGCCA

6CTCCAAT

CATGACAAAT

GGAACCAAAG

GGTAGTGA

AAAAACGTTG

GTTTACAACA

ACNCATACGC

CCAAA.AACAC

GAATTGCCGC

GCAATGCTTG

TAGAAAACAC

CGCATACNCC

TAANCATGG

AAGGGTACGA

TO-GATGATTA

CTAAAGGGGN

TGACNATNTC

GTGTAACNCT

AACAAAATAC

MAANCCTAA

CAATCAAATT

ACCCTCGNGT

TTAGTGGCCA

TGA-AMRAGG

AAATCACGGC

CACACTCATG

CAATCGAAGA

CAGAAAAACC

CGCA.AGCACC

AAACAGGCGA

GCTTACCAGG

GCAAACAGAA

TGATGATCCA

AAAAGTNGCT

TXAAGACTTT

CGATGAAACG

AGACATTACO

TGCCAAAGAC

CCCTACAAAA

ATTGGNCAA

AAAAGACGTG

AGGGGAGAAN

TGTGGAAGAA

ATGGTCTGTG

GGACGACATT

CAGTCAAGCC

GAAAGCGTTC

ATCTTTCAAC

ACAAACNCCA

AGTAGAGCCA

AAAACAAAAT

CTTACGCTTT

AAGAAACAAA ATAA 0* 0@

C

EF062-2 (SEQ ID NO:234) MKAXK QYKTYKAKNH WVTVPILFLS VLGAVGLATD NVQAAELDTQ PETrVQPNN

PDLQSEKETP

AEKSAQEQPV

ITVVEKPAED

KNKAEKERXX

FNKVNAGDSK

LNASYLGRKI

FFDASGKEVL

GK FYS PED ID

SSAQWFAFXT

NDLNVXRGDS

NXKDXKAVAA

VKNVEGDFEN

TKIYYEVKSS

ISAYILLENK

YNKELVRSNT

FDTVDLATGV

QAFILAXGGQ

IKVGDKQSQN

ANSNFVLADG

GVERIAAGDV

VVEKASVVPE

KTAVSEE-ATV

VSPETTNEPL

LGNVSSKDLA

KEVAEYNKHK

DIFTKLRKDM

TKAEFVYELQ

PDKDSPFAYA

YGTGPSGLKN

NLNAQSVKP I

LQYIVTGDTT

AINSGXAKDV

TAVQEJTXDGE

ERPANYGGXT

DNKDLTFTMN

VVTHTPDDPK

SFFDDYDETX

ELRVTLPTKV

GATIKLGEXF

TKVNKGDDI S

YNTIEESFN

LPQTGEKQNV

QKDTTSQPTK

GQPTEVAPAE

AKEKEVDQLQ

NENSYVNEAI

GGKXTGNFQN

SSPSQSGTLN

LSSLNSSLTN

SDWDAVGHKN

FNYGNPKEPE

ELAKVDPKTV

TASYXLNLDQ

TVTNTVINH'V

EEWGMNDVLD

QALLAALNEG

PTKAVHNKKG

VrP IKDLLRV

KADVSGDVYN

FYEFTSSDI P

KLFTMTFEQG

EKIKTNTVVT

LLTVAGSLAA

VEEVAPE:NKG

NEVNKSTSIP

KEQAKI IAQO SKNLVFrDQSV

SFVKEANLGS

AVFSNDPIIT

KGGHAEFVSD

AYFGSGVGLA

KATIEFNXYK

TKQGIRDTFO

NTVTAMMY(TN

PGSNPSKDVK

TTHDRFTGKW

SNKVGKQAWS

EXIXHGKVAR

KDSKGXDITN

SAEQNTFGQR

AEYAGVVEEW

VVKITASQAF

HTPEKPQTPP

MLGLAGLGFK

TEQS SATPND

KEFETPDVDK

AAELKAKt-EK V'rKDTKISSI

NGGYAVLLEK

AF IGTNRVNG

FGANNAFKYI

NGRI SFSFGM

ANVVPVLVPN

AEKV'rIDLSK

ADGSWVLAMG

ADKNG'PJGSV

HAITXYDLKV

VYLEVERXKT

GDVLSYEMTW

QFTI SWDDAK

IKTNTVVNHI

S ISDKLDVKH

XDAMNLKENK

EK'IVIVPPTP

RRKETK

'ITNAQQPTVG

AVDEVKKDPN

IAKENAEIAA

KGGKF IKATD

NKPVTVTYTG

KDVKTRLTIK

NGSYVKKQAD

T'rKGKSNVPV KEV'rDGQKNX

VKVYQADASL

YKYLLVLPFV

SLHDKDIPLQ

GXKTLKAGTD

GDVENTQTEN

OLKGYDKDFA

GTVTXSAKDP

PKVXPKKDVV

DKFSGQWSVF

NVAHS WRA I

KTPQAP'JEPL

EF062-3 (SEQ ID NO:235) TGATTCTTGA AGCAACAAAT GAAAGCAAAA AAACAATATA AGACATATAA AGCTAAGAAT CACTGGGTAA CTGTCCCTAT TCTTTTCTA AGTGTGTTAG GAGCCGTAGG ATTAGCTACT 139 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

GATAATGTAC

AACCCCCACC

GTACAAAAAG

GGTACTCAAC

GGAGCTGAAA

CTAGGGCAGC

CCTAAAGAAT

AACATTACCG

GCTGCAAAAG

CAAGCAGCTG

GCAA.AAAACA

AAGAACGAA.A

GTCGTGACGA

GATT=AATA

ATCGGNGGGA

TCTAATGGTG

GGACTAAACG

CAATCCTCAC

ACNGCTT'ITA

AAGTTCTITG

GCGCTGTCTT

GA TI'TGGGG

GATGGAAAAT

AATAGTGATT

GCNAATGGNC

GTATCTAGTG

ATTTCALATT

AAAGCCAATG

NTCAATGATT

ACAGAACTTG

GATGCAGAAA

CTNAACGANA

GTGACTGCTT

AACGCNGACG

GTAGTGA-AAA

GAAACGGTAA

AAAGCAGATA

CAAACAAAAA

ACNGAAGAAT

TGGCACGCTA

GATATTTCTG

AATCAAGCAT

TCTGTGTATC

AACTACAACA

AAACCAACCA

CGTGGTGATG

GCCTN'rATA

AANCTGACAC

AACCAGTCP

CCACAAGCCI

GTCAA.AGCCC

CGAATTAAAP

GTITATI'AAAC

TTCTTCTAT'C

AAGCCGCGGA

TGCAGTCAGA

ACACTACTI'C

AAAGTTCAGC

AATCAGCACA

CAACAGAACT

TTGAAACACC

'rrGTTGAAAA

AAAA.AGAAGT

AATTAAAAGC

AAGCNGAAAA

ACAGCTATGT

AAGACACTAA

AAGTAAATGC

AAGNTACTGG

GGTATIGCGGT

CTAGTTAT

CAAGCCAAAC

TTGGTACAAA

ATGCGTCAGG

CTTTAAATTC

CNAACAATGC

TTTACTCACC

GGGACGCTGT

CTATTTCCTT

CGCAATGGTT

ATGGGAATCC

TCGTTCCTGT

TAAATGTGAA

CCAAAGTAGA

AAGTGACGAT

AAGACTNAAA

CTTATGANCT

GNTCNGTTGT

ATGTAGAAGG

CAAATACAGT

AAAACGGTAC

TTTATTATGA

GGGGCATIGAA

TTACNAANTA

CCTACATTCT

TATTGGCNC

TGGAAGTCGA

AAGAGCTTGT

*AAGCCGTTCA

TTCTITCTTA

*CAGTCGATCT

CAATCAAAGA

*CGATCTCNTG

TTA~rCTAGC

ATGTTTCTGG

LCCAATACNG'I

TNCGTGACAA

AATTTACAACG

AT'TAGATACG C AAAGGAAACA

TCAACCGACC

TACCCCAAATC

AGAACAACCA

TGCACCAGCT

AGACGTTGAT

ACCAGCAGAA

AGACCAACTA

CAAAAATGAA

AGAGCGNTAN

CAATGAAGCG

AATTTCGTCG

AGGGGATTCA

CAACTTCCAG

TC=TTAGA.A

AGGACGTAAA

TGGAACGI!EA

CAGAGTCAAT

TAAAGAAGTA

AAGTTAACG

GTTCAAATAC

GGAAGATATT

AGGTCACAAG

TTCTTTTGGT

TGCCTTTAGN

AAAAGAACCA

NCTTGTGCCN

NCGTGGCGAT

TCCAAAAACA

TGATI1TATCC

AGCTCTTGCT

CAATTTAGAT

TTI'AGCAATG

CGATTFI'GAA

GATTAACCAT

AGTTGGCAGT

AGTGAAATCT

TGATGTCTTG

TGACC~rAAA

TTIAGAAAAC

NTTAAATCAA

ACGGATNAAA

NCGTTCTAAT

TAACAAGAAA

TGAAATGACN

TGCCACAGCC

CTTACTTCGT

GGACGAT--CC

GNATGGTGGG

NAGTTTAT

TGTCAACCAT

ACAAAGTCAA

TAGTCACATT

A.ACCAGAAA C CTAAAACGG C

LAACTAGAAG

;ATAccACAA I~ 3TAGTAACCCC

.AAAATCAAC

VAAGCAGTTG2 'AC'1rAGG3CA2 CAAAAAGAAC2 A.AAATTGCCA2

GANAAAGAAG

C.T'PAGTAAAA

ATTAAAGGCG

~AAGCATATCT

~A ETCCTI=

AAAAATAAAC

ATTACAAAAG

AATGCAGTAT

GGTAAGGATG

CTACCAGATA

A.ATAAAGGTG

ATI'AATCGNT

GACTATGGCA

AATCCCTACT

ATGACAACAA

ACTAACTTAA

GAAAAAGCAA

A.ATAAAGAAC

TCTTTACAAT

GTLAACNAAAC

AA.AGTGAAAG

GCAGCNA TrA

CAAAACACCG

GGGTATAAAT

ALATACAGCTC

GTGCCAGGTA

GTTTCTCTAC

TCCGAACGTC

GACACGACCC

GTAGGGGANA

AAACACAATA

GGAAGCAATA

ACAGGTGACG

ACNGTGGTGA

GGGGAAGANA

TGGGACTTAA

G'TTCTTTCT

CTCAAACATT

AAAGGCACGG

CAAGAATTGC

AATTCAGCGG

ATTCCAAAAG

AATGGNGCCA

CCTGCAGAAT

AACGACGGT TI ACTATCTGA A UkGTACCGCC A CGCGCkACA A :TGAAACAAc C rCAATAAALTC kTGAAGTAAA kCGTTTCTTC k.AGCGAAAAA G %AGAAAATICC A LCGCNGAATA C kCCTAGCTT C 7,AAAATTTAT C rTACAA.ATT I

TAAAAGAGC

CAGTGACAGTC

CAGAA--TTGT

I'rTCAAACGA

FTAAAACACG

AAGATAGTCC

GCCATGCGGA

CNTIATGTGAA

CAGGACCTTC

TTGGTTCAGG

AAGGAAAAAG

ATCCAATC

CGATTGAATT

TCACTGATGG

ACATITGTZAC

AAGGGA TTCG

TTTATCAAGC

ATTCAGGAAN

TCACAGCAAT

ATI'TAC?M~T

TTCAGCTGAC

GTAATCCTTC

ATGATAAAGA

CAGCNAACTA

ATGATCGTTT

AAACGTTAAA

AAGACTTGAC

AACTAGGCAA

TAGAAAACAC

CGCATACNCC

TTAANCATGG

AAGGGTACGA

TCGATCATTA

CTAA.AGGGGN

TGACNATNTC

GTGTAACNCT

AACAkAATAC

TGAANCCTAA

CAATCAAATT

ACGCTGCNGT

CAACCCAAT

GAAGCAACA

GAAAATAAA

.CCAACAGTA

AATGA.ACCT

LACGTCCATT

AAAGATCCA

'AAAGATTTA

ATTCCCCAA

GCAAATTGCG

:AACAAGCAT

:GATCAATCT

:AAAGCAACT

~CGGAAAGAT

LkATC ETGGG

~ACCTATACA

rrATGAACTA

ICCGATTATC

YIAACGATT

kTTTGCGTAT AT'rrGTTTCT

AAAACAAGCG

TGGATTGAAA

TGTAGGTCTA

rAATGTCCCT AGfTGAAGCCT

CAATNGATAC

NCAGAAAAAT

AGGGGATACC

AGATACNTT

AGACGCAAGT

ACCTAAAGAC

GATGAAAACC

CTTGCCGTTr

AAANGATGGN

CAAAGATGTA

TATTCCG ITA

TGCGGAATN

CACAGGNAAA

AGCAGGAACA

cI'rACNATG

ACAAGCTTGG

GCAAACAGAA

TGATGATCCA

AAAAGTNGCT

TAAAGACTTT

CGATGAAACG

AGACATTACG

T~cCAAAGAC

CCCTACAAAA

AFT=GNCAA

AAAAGACGTG

AGGGGAGAAN

TGTGGAAGAA

140 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

TGGTCGATTA

T1'TGCCAATT

TCGAAACTAT

TTrINGATG

ATTGGTGTAG

XATCACAAGA

CCAGAAAAAA

GCGATAAACT

CTAA'T=T

TCACGATCAC

CGATGAATCT

AACGAATTGC

TTAAA-ACNAA

AGACGTCAAA

TTITAGCAGAC

CTTTGAACAA

AAAAGA.AAC

GGCAGGAGAC

TACGGTAGn-G

CATGACAAAT

GGAACCAAAG

GGGGTAGTCA

AAAAACGTTG

GTTACAACA

ACNCATACGC

CCAAAAACAC

TTAGTC-CCCA

TGAATAAAGG

AAATCACGGC

CACACTCATG

CAATCGAAGA

CAGAAAkAACC

CGCA-AGCACC

ATGGTCTGTC

GGACGACATT

CAGTCAACCC

GAAAGCGTTC

ATCTTTCAAC

ACAAACNCCA

AGTAGAGCCA

CAGTGATTGT ACCACCAACA TTAGTGGTAG AAAAGGCAAG MO EF062-4 (SEQ ID NO:236) AELDTQ PETTTVQPNN PDLQSEKETP KTAVSEEATV AENSAQEQPV VSPETTNEPL ITVVEKPAED LGNVSSKDLA KNKAEKERXX KEVAEYNKHK FNKVNAGOSK DIFTKLRKOM LNASYLGRKI TKAEFVYELQ FFDASGKEVL PDKDSPFAYA GKFYSPEDID YGTG?SGLKN SSAQWFAFXT NLNAQSVKPI NDLNVXRGbs LQYIVTGDTT NXKDXKAVAA AINSCXAKDV V-KNVEGDFEN TAVQLTXDGE TKIY-YEvKSS ERPANYGGXT I SAY ILLENK DNKDLTFTMN YNKELVRSNT VVTHTPDDPK FDTVDLATGV SFFDDYDETX QAFILAXGGQ ELRVTLPTKV IKVGDKQSQN GATIKLGEXF ANSNFVLADG TXVNKGDDIS CXTERIAAGDv YNTIEESFNN

VVEKASV

QKDTITSQPTK

GQPTEVAPAE

AKEKEVDQLQ

NENSYVNEAI

OOKXTONFQN

SSPSQSGTLN

LSSLNSSLTN

SDWDAVGHKN

FNYGNPKEPE

ELAKVDPKTV

TASYXLNLDQ

TV'TNTVINHV

EEWGMN~DVLO

QALLAALNEG

PTKAVHNKKG

VTP IKDLLRV

KADVSGOVYN

FYEFTSSDIP

KLFTMTFEQG

EKIKTNTVVT

VEEVAPENKG

NEVNKSTS IP KEQAKK IAQQ

SKNLVFDQSV

SFVXKEANLGS

AVFSNDPI IT

KGGHAEFVSD

AY'FGSGVGLA

KATIEFNXYK

TKQGIRDTFD

NTVTAMMKTN

PGSNPSKDVK

TTHDRFTGKW

SNKVGKQAWt

EXIXHGKVAR

KDSKGXD ITN

SAEQNTFGQR

AEYAGVVEEW

VVXITASQAF

HTPEK(PQTPP

TEQS SATPND

KEFETPOVDK

AAELKAKNEK

VTKDTKISSI

NGCYAVLLEK

AF IGTNRVNG FGANNAFKY I NOR ISFSFOM

ANVVPVLVPN

AEKVTIDLSK

ADGSVLAMG4

ADKNCTVGSV

HAITXYDLKV

VYLEVERXYXT

GDVLSYEMTW

QFTISVIDDAK

IXTNTV/VNH I S ISDKLDVKH XDAI1JLKENK

EKTVIVPPTP

TTNAQQPTVG

AVDEVKKDPN

IAKENAEIAA

KGGKF IKATD

NKPVTVTYTO

KDVKTRLTIK

NGSYVKKQAD

TTKGKSNVPV

KEVTDGQKNX

VKVYQADASL

YKYLLVLPFV

SLHDKDIPLQ

GXKTLKAGTD

GDVENTQTEN

DLKGYDKDFA

GTVTXSA.KDP

PKVXPKKDVV

DKFSGQWSVF

NVAHSWKAF I

KTPQAPVEPL

S

EF063-1 (SEQ ID NO:237) TGA'rTCTTGA

CACTOGGTAA

GATAATGTAC

AACCCCGACC

GTACAAAAAG

GGTACTCAAC

GGAGCTGAAA

CTAGGGCAGC

CCTAAAGAAT

AACATTACCG

GCTGCAAAAG

CAAOCAGCTG

GCAAAAAACA

AAGAACGAAA

GTCGTGACGA

GATTTTAATA

AGCAACAAAT

CTGTCCCTAT

AAGCCGCGGA

TOCAGTCAGA

ACACTACT'C

AAAGETCAGC

AATCAGCACA

CAACAGAAGT

TTGAAACACC

TTO-TTAAAA

AAAAAGAAGT

AATTAAAAGC

AAGCNGAAAA

ACAGCTATGT

AAGACACTAA

AAGTAAATGC

GAAAGCAAAA

TCTTTTTCTA

AJTAGATACG

AAAGGAAACA

TCAACCGACC

TACCCCAAAT

AGAACAACCA

TGCACCAGCT

AGACGTTGAT

ACCAGCAGAA

AGACCAACTA

CAAAAATGAA

AGAOCGNTAN

CAATGAAGCG

AA'TTCGTCG

AGGATTCA

AAkACAATATA

AGTGTGTTAG

CAACCAGAAA

CCTAAAACGG

AAAGTAGAAG

GATACCACAA

CTACTAAGCCC

GAAkAATGAAG AAAGCAG7M

GACTTAGCCA

CAAAAAGAAC

AAAATTGCCA

GANAAAGAAG

ATTAGTAA.AA

ATTAAAGGCG

AAAGATATCT

AGACATATAA

GAGCCGTAGG

CAACGACGGT

CAGTATCTOA

A-AGTAGCCC

ACCCGCAACA

CTCAAACAAC

TGAATAAATC

ATGAAGTAAA

ACr.'ITCTTC

AAGCGAAAAA

AAGAAAATGC

TCGCNGAATA

ACCTAGCTT

GAAA.AMTAT

TTACAAAATT

AGCTAAGA-AT

ATTAGCTACT

TCAACCCAAT

AGAAGCAACA

AGAAAATAAA

ACCAACAGTA

CAATOAACCT

AACGTCCATT

AAAAGATCCA

TAAAGATTTA

GATTOCCCAA

AGAAATTGCG

CAACAAGCAT

CGATCAATCT

CAAAGCAACT

ACGGAAAGAT

TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

p p p p

ATGGGNGGGA

TCTAATGGTG

tGGACTAAACG

CAATCCTCAC

ACNGCTITA

AAGTTCTTTG

GCGCTGTCT

GATTTTGGGG

GAT'GGAAAAT

AATAGTC-ATT

GCNAAT'GGNC

GTATCTAGTG3

AT=~CAATT

AAAGCCAATG

NTCAATGATT

ACAGAACTG

GATGCAGAAA

CTNAACGANA

GTGACTGCTT

AACGCNGACG

GTAGTGAAAA

GAAACGGTAA

AAAGCAGATA

CAAACAAAAA

ACNGAAGAAT

TGGCACGCTA

GATATTTCTG

AATCAAGCAT

TCTGTGTATC

AACTACAACA

AAACCAACCA

CCTGCTCATG

GCCTTTGATA

AANGTGACAC

AACCAGTTCA

CCACAACCCT

GTCAAAGCCG

CGAATTAAA,

GTTATTAAAG

TTCTTCTATG

TCCTCGATT~A

TTTGCCAA'rr

TCGAAACTAT

TITTTNGATIG

AITGrGTG.TAG

AATGAGAAGA

CCAGAAAAAA

TAGTGGTAG

GTCTTATTA.A

AAACGTAGAA

AAGNTACTGG

GGTATGCGGT

CTAGTTATTT

CAAGCCAAAG

TTGGTACAAA

ATGCGTCAGG

CTTTAAATTC

CNAACAATGC

TTTACTCACC

GGGACGCTGT

GTA=IICCTT

CGCAATGGTT

ATGAATCC

TCGTI'CCTGT

TAAATGTGAA

CCAAAGTAGA

AAGTCACGAT

AAGACTNAA-A

CTTATGANCT

GNTCNGTTGT

ATGTAGAAGG

CAAATACAGT

AAAACGGTAC

TTATTATGA

GGGGCATGAA

TTACNAANTA

CCTACAITCT

TATGGCNGC

TGGAAGTCGA

AAGAGCTTGT

AAGCCGTTCA

TTC'ITTCTTA

CAGTCGATCT

CAATCAAAGA

CGATCTCNTG

TTATT:ZTACC

ATG=TCTGG

CCAATACNGT

TNGGTCACAA

AATTACAAG

GCGATAAACT

CTAATTTTGT

TCACGATGAC

CGATGAATCT

AACGAATTGC

TTAAAACNAA

CAGTGATTGT

AAA.AGGCAAG

CGGTAGCTGG

CAACI"TCCAG

TCTTTTAGAA

AGGACGTAAA

TGGAACGTTA

CAGAGTCAAT

TAA.AGAAGTA

AAGTTTAACG

GTTCAAATAC

GGAAGATATT

AGGTCACAAG

TTCTTTTGTGT

TGCCTIAGN

AAAAGAACCA

NCTT~GTGCCN

NCGTGGCGAT

TCCAAAAACA

TGATT1TATCC

AGCTGTTGCT

CAATI'TAGAT

'FITAGCAATG

CGATTTGAA

GAFI'AACCAT

AGTTGGCAGT

AGTGAAATCT

TGATGTCTTG

TCACCTTAAA

TTTAGAAAAC

NTTAAATGAA

ACGGATNAAA

NCGTTCTAAT

TAACAAGAAA

TGAAATGACN

TCGACAGGC

CTTACTTCGT

GGACGATGCC

GNATGGTCGG

NGATG=rAT

TGTCAACCAT

ACAAAGTCAA

TAGTGACATT

AGACGTCAAA

TTTAGCAGAC

CTTGAACAA

AAAAGAAAAC

GOCAGGAGAC

TACGGTAGTG

ACCACCAACA

TGTNGTGCCA

TAGTTTAGCC

AATTCCTTI'G

A.AAAATAAAC

ATTACAAAAG

AATGCAGTAT

GGTAAGGATC

CTACCAGATA

AATAAAGGTG

ATI'AATGGNT

GACTATGGCA

ALATGCCTACT

ATGACA-ACAA

ACTAACTTAA

GAAAAAGCAA

AATAAAGAAG

TCTTTACAAT

GTAACNAAAC

AAAGTGAAAC

GCAGCNATTA

CAAAACACCG

GGGTATAAAT

AATACAGC 1G

GTGCCAGGTA

GTTTCTCTAC

TCCGAACGTC

GACACGACCC

GTAGGGGANA

AAAGACAATA

GGAAGCAATA

ACAGGTGACG

ACNGTGGTGA

GGGGAAGANA

TGGGACTTAA

GTTTCTTCT

GTCAAAGATI'

AAAGGCACGG

CAAGAATTC

AATTCAGCGG

ATTCCAAAAG

AATGGNGCCA

CCTGCAGAAT

CATGACAAAT

GGAACCAAAG

GGGGTAGTGA

AAAAACG~rG

GTI'TACAACA

ACN'CATACGC

CCAAAAACAC

GAATTOCCGC

GCAATGcTrG rAAAAGAGGC

CAGTGACAGT

CAGAA=TGT

rTTCAAACGA rTAAAACACGC

A.AGATAGTCC

GCCATGCGGA

CNTATMTGAA

CAGGACCTTC

TTGGTTCAGG

1AGGCAAAAAG ATGCGCAkATC

CGATTMAAT

TCACTGATGGI

ACATTGTGAC

AAGGGATTCG

TTTATCAAC

ATCAGGAAN

TCACAGCAAT

ATTACTTGT

TTCAGCTGAC

GTAATCCTTC

ATGATAAAGA

CAGCNAACTA

ATGATCGTTT

AAACGTTAAA

AAGACTTGAC

AAGTAGGCAA

TAGAAAACAC

CGCATACNCC

TTAANCATGG

AAGGGTACGA

TCGATGATTA

CTAAAGGGGN

TGACNATNTC

GTGTAACNCT

AACAAAATAC

TGAANCCTAA

CAATCAAATT

ACGCTGGNGT

TTAGTGGCCA

TGAATAAAGG

AAATCACGGC

CACACTCATG

CAATCGAAGA

CAGAAkAAACC

CGCAAGCACC

AAACAGGCGA

GCTTAGCAGG

kAATCTTGGG

;ACCTATACA

rTATGAACTA rCCGATTATC

:TTAACGATT

%.TTTGCGTAT

%.T?1GTTTCT

AIAAACAAGCG

TGGA ITGAAA

TGTAGGTCTA

TAATGTGCCT

A.GTGAAGCCT

CAATNGATAC

NCACAAAAAT

AGGGGATACG

AGATACNTT

A.GACGCAAGT

AGCTAAAGAC

GATGAAAACC

C IPGCCGTTT

AAANGATGGN

CAAAGATGTA

TA PTCCGTTA

TGGCGGAATN

CACAGGNAAA

AGCAGGAACA

GTITACNATG

ACAAGCTTGG

GCAAACAGAA

TCATCATCCA

AAAAGTNGCT

TAAAGACTTT

CGATGAAACG

AGACATTACG

TGCCAAAGAC

CCCTACAA.AA

AITTTCGNCA.A

AAAAGACGTG

AGGGGAGAAN

TrGTGGAAGAA

ATGG.TCTGTG

GGACGACATT

CAGTCAAGCC

GAA.AGCGTTC

ATC'TTTCAAC

ACAAACNCCA

AGTAGAGCCA

AAAACAAAAT

CTTAGGCTTT

AAGAAACAAA ATAA EF063-2 (SEQ ID NO:238) 142 TABLE 1. Nucleotide and Aniino Acid Seqeuences of E. faecalis Genes.

MKAKK QYKTYKAKNH WVTVPILFLS VLGAVGLATD NVQAAELDTQ PETTTVQPNN

PDLQSEKETP

AEKSAQEQPV

ITVVEKPAEID

KNKAEKERXX

FNKVNAGDSK

LNASYLGRXI

FFDASGKEVL.

GKFYSPEDID

SSAQWFAFXT

NDLNVXRCDS

NXKDXKAVAA

VKNVE OF EN TX IYYEVKSS

ISAYILLENK

YNKELVRSNT

FDTVDLATGV

QAF ILAXGGQ

IKVGDKQSQN

ANSNFVLADG

GVERIAAGDV

VVEKASVVPE

KTAVSEEATV

VSPETTNEPL

LGNVSSXDLA

KEVAEYNKH(

DIFTKLRKDM

TKAEFVYELQ

POKDSPFAYA

YGTGPSGLKN

NLNAQSVKP I LQY IVTGDTr

AINSGXAKDV

TAVQLTXOGE

ERPANYGGXT

DNKDLTFTMN

VVT}ITPDDPK

SFFDDYDETX

ELRVTLPTKV

GATIKLGEXF

TKVNI(GDOI S

YNTIEESFNN

LPQTGEKQNV

QKDTTSQPTK

GQTEVAPAE

AKEKEVDQLQ

NENSYVNEAI

GGKXTGNFQN

SSPSQSGTLN

LSSLNSSLTN

SDWDAVGHKN

FNYGNPKEPE

ELAXVOPKTV

TASYXLNLDQ

TVTNTVINHV

EEWNNDVLD

QALLAALREG

PTKAVHNKKG

VTPIKDLLRV

KADVSGDVYN

FYEFTSSDIP

KLFTMTFEQG

EX IKTNTVVT

LLTIVAGSLAA

VEEVAPENXG

NEVNKSTSIP

KEQAKXIAQQ

SKNLVFDQSV

SFVKEANLGS

AVFSNDPIIT

KGGRAEFVSD

AYFGSGVGLA

:(ATIEFNXYI(

TKQGIRDTFO

RItVTAMMKTN

PGSNPSKDVK

TTHDRFTGKW

SNKVGKQAWS

EXIXHGKVAR

KDSKGXDITN

SAEQNTFGQR

AE-YAGVVEEW

VVKITASQAF

HTPEKPQTPP

MLGLAGLGFK

TEQSSATPND

KEFETPDVDK

AAELXAKNEK

V'flCDTKISS I

NGGYAVLJLEK

AFIGTRVNG

FCANNAFKYI

NGRISFSFGM

ANVVPVLVPN

AEXVTIDLSK

ADGSVVLAM'G

ADKNGTVGSV

HAITXYDLKV

VYLEVERXKT

GDVLSYEMTW

QFTI SWDDAI( IKTNM-- VNH I

SISDKLDVKH

XDAIThNLKENK

EKIVIVPPTP

RRKETK

7TNAQQPTSIG

AVDEVKKDPN

IAKENAEIAA

KGGKFIKATD

NKPVTVTYTG

KDVKTRLTIK

NGSYVKKQAD

TTKGKSNVPV

KEVTDGQ-KNX

VKVYQADASL

YKYLLVLPFV

SLHDKDIPLQ

GXXTLKAGTD

GIJVENTQTEN

DLJKGYDKDFA

G'IVTXSAKDP

PKVXPKXD)VV

DKFSGQWSVF

NVAHSWKAF I

KTPQAPVEPL

EF063-3 (SEQ ID NO:239) GGA ATTAGATACG CAACCAGAAA CAACGACGGT TCAACCCAAT

C

AACCCCGACC

GTACAAAA.AG

GGTACTGA.AC

GGAGCTG-AAA

CTAGGGCAGC

CCTAAAGAAT

AACATTACCG

GCTGCAAAAG

CAAGCAGCTI

GCAAAAAACA

AAGAACGAAA

GTCGTGACGA

GAFTTTAATA

ATCOGNGGGA

TCTAATrGGTG

GGACTAAACG

CAATCCTCAC

ACNGCTTTTA

AAGTTCTTTG

GCGCTGTCTT

GATrrTTGGC

GATGGAAAAT

AATAGTGATT

CAATfGGNC

GTATCTAGTG

ATTTTCA-ATT

TGCAGTCAGA

ACACTACT'C

AAAGTTCAGC

AATCAGCACA

CAACAGAAGT

TTGAAACACC

TTGTTGAAAA

AAAAAGAAGT

AATTAAAAGC

AAGCNGAAAA

ACAGCTATGT

AAGACACTAA

AAGTAAATGC

AAGNTACTGG

GGTATCGCT

CTAGTTATT

CAAGCCAAAG

TTGGTACAAA

ATGCGTCAG

CTTTAAAI'C

CNAACAATC

TTTACTCACC

GGGACGCTGT

GTATTTCCTT

CGCAATGGTT

ATGOGGMTCC

AAAGGAA.ACA

TCAACCGACC

TACCCCAAAT

AGAACAACCA

TGCACCAGCT

AGACGTTGAT

ACCAGCAGAA

AGACCAACTA

CAAAAATGAA

AGAGCGNTAN

CAATGAACCC

AATL'CGTCG

AGGGGATICA

CAACTTCCAG

TCTTTTAGAA

AGGACGTAAA

TGGAACGTTA

CAGAGTCAAT

TAAAGAAGTA

AAGTTTAACG

GTTCAAATAC

GGAAGATATT

AGGTCACAAG

TTCTTTTGCT

TGCCTTTAGN

AAAAGAACCA

CCTAAAACGG

AAAGTAGAAG

GATACCACAA

GTAGTAAGCC

GAAAATGAAG

AAAGCAG LTG

GACTTAGGCA

CAAAAAGAAC

AAAATTGCCA

GANAAAGAAG

ATTAGTAAAA

ATTAAAGGCG

AAAGATATCT

AATTCCTITG

AAAAATAAAC

ATI'ACAAAAG

AATGCAGTAT

GGTAAGGATG

CTACCAGATA

AATAAAGGTG

ATTAATGGNT

GACTATGGCA

AATGCCTACT

ATIACAACAA~

ACTAACTTAA

GAAAAAGCAA

CAGTATCTGA

AAGTAGCGCC

ACGCGCAACA

CTGAA.ACAAC

TGAATAAATC

ATGAAGTAAA

ACGTTITTC

AAGCGAAAAA

AAGAAAATGC

TCGCNGAATA

ACCTAGTGTT

GAAAATTTAT

'ITACAAAAT'

TAAAAGAGGC

CAGTGACAGT

CAGALT'rGT

TI'TCAAACGA

TTAAAACACG

AAGATAGTCC

GCCATGCGGA

CNTAT'CTGAA

CAGGACCTTC

TI'GGrI'CAGG

AAGGAAAAAG

ATr-CGCA.ATC

CGATTAAT

AGAAGCAACA

AGAAAATAAA

ACCAACAGTA

CAATGAACCT

AACGTCCATI7

AAAAGATCCA

TAAAGAITTA

GATTGCCCA-A

AGAAATTGCG

CAACAAGCAT

CGATCAATCT

CA.AAGCAACT

ACGGAAAGAT

AAATCTTGGG

GACCTATACA

TTATG-AACTA

TCcGATTATC

CTTAACGATT

ATTTGCGTAT

ATTTGTrCT

AAAACAAGCG

TGGATTGAAA

TGTAGGTCTA

TAATGTGCCT

AGTGAAGCCT

CAATNGATAC

TABLE 1. Nucleotide and Amino Acid Seqeuenccs of E. faecalis Genes.

AAAGCCAATG TCGTTCCTCT NCT'TGTCCCN AA4TAAAGAAG NTCAATGATT TAAATGTGAA NCGTGGCGAT TC=TTACAAT ACAGAACTTG CCAAAGTAGA TCCAAAA.ACA GTAACNAAAC CATGCAGAAA AAGTGACCAT TGAITI'ATCC AAAGTG TCACTGATGG NCAGAAAAAT ACATTGTCAC ACGGGATACG AAGGGATTCG AGATACNTT EF063-4 (SEQ ID NO:240) ELDTQ PEITI'VQPNN

PDLQSEKETP

ARK SAQEQ PV

I'VVEKPAED

KNKAEKE.XX

FNKVNAGDSK

LNASYLGRK I

FFDASGKEVL

CKFYSPEDID

SSAQWFAFXT

NDLNVXRGDS

KTAVSEEATV

VSPETTNEPL

LGNVSSKDLA

KEVAEYNKHK

DIFTKLRKDM

TKAEFVYELQ

PDKDSPFAYA

YGTGPSGLKN

NLNAQSVKP I LQY IVTGDTT

QKDTTSQPTK

GQPTEVAPAE

AKEKEVDQLQ

NE-NSYVNEAI

GGKXTCNFQN

SSPSQSGTLN

LSSLNSSLTN

SDWDAVGHKN

FNYGNPKEPE

EL.AKVDPKTV

VEEVAPENFG

NEVNKSTSIP

K-2QAKK IAQQ

SKNLVFDQSV

SFVKEANLGS

AVFSN)P I IT

KGGHAEFVSD

AYFGSGVGLA

KATIEFNXYK

TKQGIRDTFD

TEQSSATPND

KEFETPOVDK

AAELKAKNEK

VTKDTKISS I

NGGYAV'LLEK

AF IGTNRVNG

FGANNAFKYI

NCRI SFSFGM

ANVVPVLVPN

AEKVTIDLSK

TTNAQQPTVG

AVDEVKKDPN

IAKENA7EIAA KGGKF IKATD

NYPVTVTYTG

KOVKTRLTIK

NGSYVKKQAD

TKGKSNVPV

KEVTD)GQKNX

v1 EF064-1 (SEQ ID NO:241) a a C a a a. a a a.

TGATTCTTGA

CACTGGGTAA

GATAATGTAC

AACCCCGACC

GTACAA.AAAG

GGTACTGAAC

GGAGCTGAAA

CTAGGGCAGC

CCTAAAGAAT

AACA'FrACCG

GCTCAAA.AG

CAAGCAGCTG

GCAAAAAACA

AAGAACGAAA

GTCGTGACGA

GATTTAATA

ATGGGNGGGA

TCTAATCGGTG

GGACTAAACG

CAATCCTCAC

ACNGCTTTTA

AAGTTCTTTG

GCGCTGTCTT

GXT=GGGO

GATGGAAAAT

AATAGTGATI'

GCNA.ATGGNC

GTATCTAGTG

AITIIICAAT'

AAAGCCAATG

ACCAACAA.AT

CTGTCCCTAT

AAGCCGCGGA

TGCAGTCAGA

ACACTACTTC

AAAGTTCAGC

AATCAGCACA

CAACAGAAGT

TTGAAACACC

TTGTTGAAAA

AAAAAGAAGT

AATTAAAAGC

AAGCNGAAAA

ACAGCTATGT

A.AGACACTAA

AAGTAAATGC

AAGNTACTGG

CGTATGCGGT

CTAGTTATIT

CAAGCCAAAG

TTGGTACAAA

ATGCGTCAGG

C'ITTAAATTC

CNA.ACAATC

TTTACTCACC

GGGACGCTGT

GTATTCCTI'

CGCAATGGTT

ATGGGAATCC

TCG'ITCCTGT

GAAAGCAAAA

TCTT=TCTA

A'FrAGATACG

AAAGGAAACA

TCAACCGACC

TACCCCAAAT

AGAACAACCA

TGCACCAGCT

AGACGTTGAT

ACCAGCAGAA

AGACCAACTA

CAAAA.ATGAA

AGAGCGNTAN

CAATGAAGCG

AATTTCCTCG

AGGGGATTCA

CAACTTCCAG

TCTTT'TAGAA

AGGACGTAKA

TGGAACGTTA

CAGAGTCAAT

TAAAGAAGTA

AAGTTTIAACG

GTTCAAATAC

GGAAGATATT

AGGTCACAAG

TTCTTTTGGT

TGCCTTTAGN

AAAACAACCA

NCI'CTCCCN

AAACAATATA

AGTGTG=IAG

CAACCAGAAA

CCTAAAACGG

AAAGTAGAAG

GATACCACAA

GTAGTAAGCC

GAAAATGAAG

AAAGCAGT7G

GACTI'AGGCA

CAAAAAGAAC

AAAATTGCCA

GANAAAGAAG

ATTACTAAAA

A'FIAAAGGCG.

AAAGATATCT

AATTCCTTrG

AAAAATAAAC

ATTACAAAAG

AATGCAGTAT

GGTAAGGATC

CTACCAGATA

AATAAAGGTG

ATTAATGGNT

GACTATGGCA

AATGCCTACT

ATGACAACAA

ACTAACTTAA

GAAAA.AGCAA

AATAAAGAAC

TCITI'ACAAT

GTAACNAAAC

AGACATATA-A

GAGCCCGTAGG

CAACGACGGT

CAGTATCTGA

AAGTAGCCCC

ACGCGCAACA

CTGAAACAkAC

TGAATAAATC

ATGAAGTAAA

ACGTTTCTTC

AAGCGAAAAA

AAGAAAATGC

TCGCNGAATA

ACCTACTGTT

GAAAAT1'TAT

TTACAAAATT

TAAAAGAGGC

CAGTrACAGT

CAGAATTTGT

TTTCAAACGA

TTAAAACACC

AAGATAGTCC

GCCATGCGGA

CNTATGTGAA

CAGGACCTTC

TTGGTTCAGG

AAGGAAAAAG

ATGCGCAATC

CGATTGAATT

TCAC7CATGG ACATrTCTCAC

AAGGGATTCG

AGCTAAGAAT

ATTAGCTACT

TCAACCCAAT

AGAAGCAACA

AGAAAATAAA

ACCAACAGTA

CAATGAACCT

AACGTCCAT

AAAAGATCCA

TAAAGATTTA

GATTGCCCAA

AGAAATTGCG

CAACAAGCAT

CGATCAATCT

CAAACCAACT

ACGGAAAGAT

AAATCrPTGGG

GACCTATACA

rTrATGAACTA

TCCGAITATC

CITAACGATT

AT=rCGTAT A'TrGTTrT

AAAACAAGCG

TGGATTGAAA

TGTAGGTrCTA

TAATGTGCCT

AGTGAAGCCT

CAATNGATAC

NcAGAA.AAAT

AGGGGATACG

AGATACN=I

NTCAATGA'IT TAAATGTGAA NCGTGCCGAT ACAGAACTTG CCAAAGTAGA TCCAAAAACA TABLE 1. Nucleotide and Ami no Acid Seqeuences of E. faecalis Genes.

GATCCAGAAA

CTNAACGANA

GTGACTGC7TT

AACGCNCACG

GTAGTGAAAA

GAAACGGTAA

AAAGCAGATA

CAAACAAAAA

ACNGAAGAAT

TGGCACGCTA

GATATTTCTC

AATCAAGCAT

TCTGTGTATC

AACTACAACA

AAACCAACCA

CGTGGTGATG

CCTTTGATA

AANGTCACAC

AACCAGTTCA

CCACAAGCCT

GTCAAAGCCG

CGAATTAAAA

GrI'ATTAAAC

TTCTTCTATC

TGGTCGATTA

TITGCCAATT

TCGAAACTAT

TTTTNGATG

ATGGTGTAG

AATGAGAAGA

CCAGAAAAAA

TTAGTGGTAG

GTCTTATTAA

AAACGTAGAA

AAGTGACGAT TGA=~ATCC AAGACTNAAA AGCTr.TTGCT CTI'ATGANCT CAATITAGAT GNTCNCTTGT TTTAGCAATG ATCTACAAGG CGA=ITrGAA CAAATACAGT GATTAACCAT AAAACGGTAC AGTIGGCAGT TTTATTATGA AGTCALAATCT GGGCCATGAA TGATGTCTTG ?I'ACNAANTA TGACCTTAAA CCTACATTCT TITAGAAAAC TATTGGCNGC NTTAAATGA-A TGGAAGTCGA ACGGATNAAA AAGACCTTGT NCGTTCTAAT AACCCjTTCA TAACAAGAAA TI'CTTCTTA TGAAATGACN CAGTCGATCT TGCGACAGGC CAATCAAAGA CTTACI1TCGT CGATCTCNTG GGACGATGCC TTATTCTAGC GNATGGTGG ATCTI'CTGG NGATCTITTAT CCAATACNGT TGTCAACCAT TNGGTGACAA ACAAAGTCAA AATTTACAAG TAGTCACATT GCGATAALACT AGACGTCAAA CTAATTTTGT TTTAGCAGAC TCACGATCAC CTTTGAACAA CGATGAATCT AAAAGAAA-AC AACGAATTGC GGCAGGAGAC 'TTAAAACNAA TACCTAGTG CAGTGATTGT ACCACCAACA AAAAGGCAAG TGTNGTGCCA CGGTAGCTGG TAGTF'ACC AAGAAACAAA ATAA

APLAGTGALAAG

GCAGCNATTA

CAAAACACCG

GGGTATAAAT

AATACAGCTG

GTGCCAGGTA

GTTTCTCTAC

TCCGAACGTC

GACACGACCC

GTAGGGGANA

AA.AGACAATA

GA-ACAAT-A

ACAGGTGACG

ACNGTGGTGA

GCGGAAGANA

TGGGACTTAA

GIT7C=TCT

GTCAAAGATT

AAAGGCACGG

CAAGAATTC

AATTCAGCGG

ATTCCAAAAG

AATGGNGCCA

CCTGCAGAAT

CATGACAA.AT

GGAACCAAAG

GGGGTAGTGA

AAAAACG.TTG

GTTTACAACA

ACNCATACGC

CCAAAA.ACAC

GAATTGCCGC

GCAATGCTTG

T'rTATCAAGC

ATTCACOAAN

TCACAGCAAT

ATTTACTTGT

TTCAGCTGAC

GTAATCCTTC

ATGATAAAGA

CACCNAACTA

ATGATCGTTT

AAACGTTAAA

AAGACTTGAC

AAGTACCCAA

TAGAAAACAC

CGCATACNCC

TTAANCATGG

AAGGGTACGA

TCGATGATTA

CTAA-A=GGN

TGACNATNTC

GTGTAACNCT

AACAAAATAC

TGAANCCTAA

CAATCAAAT

ACGCTGGNGT

TTAGTGGCCA

TGAATAAAGG

AAATCACGC

CACACTCATG

CAATCGAAGA

CAGA-AAA6ACC

CGCAAGCACC

AAACAGGCGA

GCTTAGCAGG

AGACGCAAGT

AGCTAA-AGAC

GATGAAAACC

CTTGCCGTTT

AAANGATGGN

CAAAGATGTA

TATTCCGTTA

TGGCGGAATN

CACAGGNAAA

AGCAGGAACA

GTTACNATG

ACAAGCTTGG

GCAA.ACAGAA

TGATGATCCA

AA.AACTNGCT

TAAAGAC=?

CGATGAAACG

AGACAITACG

TGCCAAAGAC

CCCTACAAAA

ATTTGGNCAA

A.AAAGACGTG

AGGGGAGAAN

TGTGGAAGAA

ATGGTCTGTG

GGACGACATT

CAGTCAAGCC

GAAAGCGTTC

ATCTI'CAAC

ACAAACNCCA

AGTAGAGCCA

AAAACAAAAT

CTTAGGCTT

ft.

ft ft.

ft ft.. ft ft.

ft ft ft ft ft ft ft...

ft ft ft...

ft ft ft.. ft ft. ft ft ft ft ft ft.

EF064-2 (SEQ ID NO:242) MKAKK QYKTYKAKNH WVTVPILFLS VLGAVGLATD NVQAAELDTQ PETTI'VQPNN

PDLQSEKETP

AEKSAQEQPV

ITVVEKPAED

KNKAEKERXX

FNKVNAGOSK

LNASYLGRK I

FFDASCKEVL

GKFYSPEDID

SSAQWFAFXT

NDLNVXRGDS

NXKDXKAVAA

VKNVEGDFEN

TKIYYEVKS

I SAYILLENK

YNKELVRSNT

KTAVSEE-ATV

VSPETTNEPL

LGNVSSKDLA

KEVAEYNKHK

D IFTKLRKDM

TKAEFVYELQ

PDKDSPFAYA

YGTGPSGLKN

NLNAQSVKP I

LQYIVTGTT

AINSGXAKDV

TAVQLTXDGE

ERPANYGGXT

DNKDLTFTN

VVTHTPDDPK

QKDTTSQPT(

GQPTEVAPAE

AKEKEVDQLQ

NENSYVNE-AI

GGKXTGNFQN

SSPSQSGTLN

LSSLNSSLTN

SDWDAVGHKN

FNYGNPKEPE

ELAKVDPK'IV

TASYXLNLDQ

TVTN'IVINHV

EEWCMNDVLD

QALLAALNEG

PTKAVHNKKG

VEEVAPENKG

NEVNKSTSI P

KEQAKKIAQQ

SKNLVFD)QSV

SFVKEANLGS

AVFSNDP IIT

KGGHAEFVSD

AYFGSGVGLA

KATIEFNXYK

TKQGIRIDTFD

NTVTAMMKTN

PGSNPSKDVK

TrHDRFTGKW

SNXVCKQAWS

EX IXHGKVAR

TEQSSATPND

KEFETPDVDK

AAELKAKNEI(

VTKDTKISS I

NGGYAVLLEK

AF IGTNRVNG

FGANNAFKYI

NGRI SFSFGM

ANVVPVLVPN

AEKVT:-DLSK

ADGSVVLAMC

ADKNGTVGSV

HAITXYDLKV

VYLEVERXKT

GDVLSYEMTW

TTNAQQPTIVG

AVDEVKKDPN

IAKENAEIAA

KGGKFIKATD

NKPVTVTYTG

KDVKTRLTIK

NGSYVKKQAD

TTKGKSNVPV

KEVTDGQKNX

VKVYQADASL

YKYLLVLPFV

SLHDKDIPLQ

GXKTLKAGTD

GDVENTQTEN

DLKGYDKDFA

145 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. facalis Genes.

FDTVDLATGV

QAFILA.XGGQ

IK'JGDKQSQN'

ANSNFVLADC

GVER IAAGDV

VVEKASVVPE

SFFDDYDETX

ELRVTLPTKV

GATIKLGEXF

TKVNKGDDIS

YNT lEES FNN

LPQTGEKQNV

VTPII(DLLRV

KADVSGDVYN

FYEFTSSDIP

KLFTMTFEQG

EKIKTNTVVT

LLTVAGSLAA

KDSKGXD ITN

SAEQNTFGQR

AEYACVVEEW

VVKITASQAF

IITPEXPQTPP

MLGLAGLGFK

QFTISWDOAK

I KTNTVVNI

SISOKLDVKH

XDAMLKENK

EXKTVIVPPTP

RRKETK

GTVTXSAKDP

PKVXPKX(DVV

DKFSGQWSVF

1NAHSWdKAF I

KTPQAPVEPL

EF064-3. (SEQ ID NO:243) AGTGACGAT TGATTTATCC AAAGTGAAAG TTTATCAAGC AGACGCAAGT C* C

CTNALACGANA

GTGACTGCT

AACGCNGACG

GTAGTGAAAA

GAAACGGTAA

AAAGCAGATA

CAA.ACAAAAA

ACNGAAGAAT

TGGCACGCTA

GATATTTCTG

AATCAAGCAT

TCTGTGTATC

AACTACAACA

AAACCAACCA

CGTGGTGATG

GCCTTTGATA

AANGTGACAC

AACCAGTTCA

CCACAAGCCT

GTCAAAGCCG

CGAATI'AAA.A

G ETATI'AAAG TTC'rrCTATG

TGGTCGATTA

?I'TGCCAATT

TCGAAACTAT

TI TTNGATG

ATTGGTGTAG

AATGAGAAGA

CCAGAAAAAA

'ITAGTGGTAG

GTC ETATTAA

AAACGTAGAA

AAGACTNAAA

CTTATGANCT

GNTCNGTTGT

ATGTAGAAGG

CAA.ATACAGT

AAAACGGTAC

TTTATTATGA

GGGGCATGAA

TTACNAANTA

CCTACATTCT

TATTGGCNGC NTTAAATGA.A TGGAAGTCGA ACGGATNAAA AAGAGCTTGT NCGTTCTAAT AAGCCCTTCA TAACAAGAAA TTCItTICTTA TGAAATGACN CAGTCGATCT TGCGACAGGC CA.ATCAAAGA CTTACTTCGT CGATCTCNTG GGACGATGCC TTATTCTAGC GNATGGTGGG ATGT=CTGG NGATGTTTAT CCA.ATACNGT TGTCAACCAT TNGGTG.ACAA ACAAAGTCAA AATI'TACAAG TAGTGACATT GCGATAAACT AGACGTCAAA CTAATTGT TTTAGCAGAC TCACGATGAC CTTTGAACAA CGATGAATCT AAAAGAAAAC AACGAATTGC GGCAGGAGAC TTAAAACNAA TACGGTAGTG CAGTGATTGT ACCACCA.ACA AAAAGGCAAG TGTNGTGCCA CGGTAGCTGG TAGT=TAGCC AAGAAACAAA ATAA

AGCTGTTGCT

CAA=TAGAT

TTAGCAATG

CGATTTI'GAA

GATTAACCAT

AGTTGGCAGT

AGTGAAATCT

TGATGTCTI'G

TGACCT'rAAA

TTTAGA-AAAC

GCAGCNATI'A

6AAAACACCG

GGGTATAAAT

AATACAGC EG

GTGCCAGGTA

GTTI'CTCTAC

TCCGAACGTC

GACACGACCC

GTAGGGGANA

AAAGACAATA

GGAAGCAATA

ACAGGTGACG

ACNGTGGTGA

GGGGAAGANA

TGGGAC'IWAA

GrrCTT'rCT

CTCAAAGATT

AAAGGCACGO

CAAGAATTGC

AATTAGCGG

ATTCCAAAC

AATGGNGCCA

CCTGCAGAAT

CATGCACAAAT

GGA.ACCAAAG

GGGGTAGTGA

AA-AAACGTM'

GTTTACAACA

ACNCATACGC

CCAAAAACAC

GAATT'C CCGC GCAATGCTrIG

ATTCAGGAAN

TCACAGCAAT

ATTTACTTGT

TTCArGtTGAC

GTAATCCTTC

ATGATAAAGA

CAGCNAACTA

ATGATCGTTT

AAACGTTAAA

AAGACTTGAC

AAGTAGGCAA ACAAGCTTGG TAGAAAACAC CCAAACAGAA CGCATACNCC TGATGATCCA TTAANCATGG AAAAGTNGCT A-AGGGTACGA TAAAGACTITT TCGATGAITA CGATGAAACG CTAAAGGGGN AGACATTACG TGACNATNqTC TGCCAAAGAC GTGTAACNCT CCCTACAAAA AACAAAATAC ATTTGGNCAA TGAANCCTAA AAAAGACGTG CAATCAAATT AGGGAGAAN ACGCTGGNGT TGTGGAAGAA 'ITAGTGGCCA ATCCTCTGTG TGAATAAAGG GGACGACATT AA-ATCACGGC CAGTCAAGCC CACACTCATC GAAAGCGTTC CAA.TCGAAGA ATC'FrrCAAC CAGAAAAACC ACAAACNCCA CGCA.AGCACC AGTAGAGCCA AA ACAGCCA AAAACAAAAT GCTTAGCAGG CTTAGGCTTI

AGCTAAAGAC

GATCAAAACC

CTTGCCGTTT

AAANGATGGN

CAA.AGATGTA

TATTCCGTTA

TGGCGGAATN

CACAGGNAAA

AGCAGGAACA

aTTTACNATG EF064-4 (SEQ ID N0:244) VTIDLSK VKVYQADASL NXKDXKAVAkA AINSGXAKDV VKNVEGDFEN TAVQLTXDGE TKIYYE'VKSS ERPANYGGXT I SAY ILLENK DNKDLTFTMN YNKELVRSNT VVTHTPDDPK FDTVDLATGV SFFDDYDETX QAFILAXGGQ ELRVTLPThV

TASYXLNLOQ

TVTNTVINHV

EEWGMNDVLD

QALLAALNEG

PTKAVHNKKG

VTP IKDLLRV

KADVSGDVYN

NTVTAMMKTN

POS14PSKDVK

TTHDRFTGKW

SNKVGKQAWS

EXIXHGKVAR

Kl:SICCXrJITN

SAEQNTFGQR

ADGSVVLAMG

ADKNG'IVGSV

HA ITXYDLK(V

V'YLEVERXKT

GDVLSYEMW

QFTISWDMDAI(

IKTNTVVNHI

YKYLLVLPFV

SLHDKD IPLQ

GXKTLKAGTD

GDVENTQTEN

DLKGYDKOFA

GTVTXSAKDP

PKVXPKKDVV

146 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

IKVGDXQSQN GATIKLGEXF FYEFTSSDIP ANSNFVLADG TKVNKGDDIS KL.FTMTFEQG GVERIAAGDV YNTIEESFNN EKIKTNIVVT

VVEKASV

AE:YAGVVEE:W

VV-KITASQAF

HTPEKPQTPP

SI SDKLDVKH DKFSGQWSVF XDAMNLXNK NVAi{SWKAPI EKTVIIVPPTP KTPQAPVEPL EF065-1 (SEQ ID NO:245)

TAGCGAAAGA

ATGGTGXITT

GAAGCGGTTA

AGTCTGGCTr,

CAAACGGAAC

TTTCCACATG

GTGATGCTGG

ATTAATTCAG

AGCCAATTTG

GGATTGAAAC

ACGTATTTCC

AAGACCAATA

TCAGTGGAAT

GAAA TTACTA

AGTI'CAGTCA

GAG ITGCAAC

TTTACAACCC

GCTTCA'rrAA

GGAAATGATG

TACGTAGGAA

ACCCTTGTAA

ACGATTCCTA

ACAAAAGATA

TGCCATGTCA

GATGACATTA

GATGTTACAG

AAACAAGCAG

AAAATTAAAA

CCCA.ACCAAG

ACCGTAACAC

TTAGATITAA

GAAACAAGCA

ACTGATGTAA

CAAGAAAATA

AGTGGTCATA

GAATTAGCAC

AACGAAGOTO

GAAGATCCAA

GACCAAGAAT

CAAGCCAGCA

GANGAAAATG

ACTTTTACTA

ATOACTATTA

GAACAAGGCG

CATTCCAACA

AAATAGGGAG

TCGCTGGAAC

AAGCAGGAGA

ATTGTAAACG

CAGTCGATI'

TAAGACAAGC

CTTCATATCG

CTGATTATGA

TCTCTGGTTT

TCGCTTTAGA

TATTAGTGAC

CCAATGATTC

ATAGTAATGA

ACCAAGGCTA

ATTCATACTI'

AAGGGTCTAG

AATTAAAACA

CGATTGCCAA

TrCCCTGTTCA

ACATCACGAT

GTAGTGGGAC

AAAAST;,CAA

TCGAAAATCA

AAACAGCCTT

ATAAAGTGCT

CTAACGGCAC

ACAGCTATGA

CTGACGCAAC

CCGACTTAAA

CACCGCCAGT

CCAACCGCGA

CTTGACCCA

AAGTCACAGA

ACAAAGTAAC

CGTACACAAT

CTTATATTGA

ACGTGTTGCA

CCATTACAAA

TTAAATGGAA

TGGTGGATGA

GCAAACATUT

TGAACAAAAA

CCACTAAAAT

GGA'ITCCCAA

AGCCAACCGT

GATTAAAATG

AGTTGTGGGA

TACAGAAGGA

GATATTGGAA

AGTAGTTCTT

GATTGATGAA

CGGCGGAAAA

TATGAATGTG

TGGAGACGTT

TACGTACAAT

AGATGGGGTC

AATCA.ATCAA

CTACCAAGGT

TGAAATGATT

TGATAAATAT

CACACCAGAA

AATTGTCAAA

TCAATTTGAT

AATTAACGGA

TCACTACGAA

AATCAATCAA

TOCGCATGCG

AGAACAC ETA

TGGCAACGAA

AGATATCATT

AGTAACACAA

CTATI'AAGT

GGACGAAGAA

CTTTGGCAAT

TGATCCAAAT

TCAAGAATTT

AGCCAGCATC

TGAAAATGGT

TTGAAAMG

GACCATTACT

ACAAGGTGGC

TTCCAACAAA

AGATATCGAA

CGTCAAAACA

CATTAATAAA

TACAGATAAT

AGATGACAGC

TAAAACTGAC

CCAAGCCGAC

AACACCGCCT

TTTAAGAAAG

,NATTr-CACTC

ATGACCAATA

GGACAAGCTA

GAAGATGCTA

GTGGTTCAAG

CAATTTATT

CGCGTCA-ATA

CGGACGTATG

CAAACACACG

GCTAATACAC

TATCCAGATC

GCAGCAGCAG

AATGCGTATr

AAAACAGAAG

GATTITATTA

GATCGTCTGG

ATTCAATCTG

CAA-ACCATT'r

GTCAAAGAAA

GGAACAATM'

TGTGACCGTCA

GATTAACCA

ACCAGTACTT

GATGCAAAG

GAAAATAACA

GGTCATACGT

TTAGCGCC'IT

GAAGTGACG

AT1'GCTAAAG

AAATCAACG

GTAGATGACA

AAAGATGTrA

AACAANCAAG

ACTAAAATCA

ATTCCCAACC

CCAACCGTAA

GG.CCAACAC

GCTTTCCGTA

GTG~rAGACA

GGCATAG-TAA

TACTCTTACT

GCA-ACGGATG

TTAAACTTTG

GCACCA.ACGC

CAACGAAATT

CCACATTOGC

CGGTC;AAAGT

C=~CCCAGT

GTGGTkGTTT

GCTTATCTGA

TT-CCTGATGG

CGCAA~rGAC

GTGGTACCC

GAGATf'A.AC

GTTTAGATGG

CAAGACATCC

AAOT'T1AGC

GGGAAAGTGT

TGGGTCCTTT

CAAGCCAATC

CGCAATCGAC

CGACCGCTAC

CAGCAACTAG

ATACAGCGAT

CTAAGGAATT

CGCCAGAAGA

ATCGTIGAAGA

GGACCCAAGC

TCACCGACGA

AAGTAACTTT

ATACAATGAC

ACATIGAACA

TGTTACATTC

ACGTAGAAGG

TCAAAACAGC

TTAATAACT

CAGCTAACGG

CNGACAGCTA

AAGCTAGCGC

AAGCCGACTT

CACCACCTGC

ATr'rAGATTT

ACGAAACAAG

TCACAGACGT

CACAAGAAAA

TAGCTGGTCA

AAGAATTAGC

GCAACGAAGG

CAGA-AGACCC

ATTATCGACA

TTTAGCTGAA

GAAAGACGAC

TCAAGCGGGT

TTCAGATAAT

r-AAGACCGC

AAAGACAAAA

TTATGATAA.A

AACCCCCCCA

GAATCGAAAA

TTACTTGCAT

TCTTCAAGTC

GTTAAACCALA

TGAATCTTTA

TCTAAAACAA

TATTATGAT

ACCAGCAACA

GGACCATr.CT

TACAGAAGGT

TGATGCAGCA

TCCAGAAGCG

TCCAACGATT

TAGTTTCGAT

CAGCATGGTG

AAATGGTAAA

TGAAATGAAC

TATCACCACT

AGGCGGGATT

CAACAAACCA

ACAAGAACAT

TTTCGGTAAC

GTTAGACATC

CAAAGTAACA

TGACTATITA

AACGGACGAA

GAACTTTGGC

ACCAACGCCA

AACCAACCCT

CACATGGACC

GAAAGT1'NCT

TAACAAAGTA

TACATACACA

GCcTTATAT

TGACGTGTTG

AAAAAAACCT

.00.

147 TABLE I. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

GAACCTAAAC AACCGCTAAA ACGAACCCAG TCAATTI'GG ACAACAGTAA ATCCAC ITA TTTGGCATAA CAAAAAATAA ACCGAAA.AAA CCGTTGACGC CTACAAATCA TCAAGCACCA AAAATCAGCA AGTAAAGGAA TTCATIACC AATGACTAAT CATGATCGCA GGTTAATTG TCCTTATAGT GGCTATTAGC AAAAAGAAAA AATAG EF065-2 (SEQ ID NO:246) MF KKATKLLSTM VIVAGTVVGN FSPTLALAEE AVKAGD'rEGM TNTVKVKDDS

LADCKRILEG

MLASYRGGKQ

LKLALDTYNQ

VEYSNDYQGA

LQQGSSTPED

NDVPVQINGQ

I PKNDNAHAC DINKVLDI ID

IKTDATDEEL

DLTNRDQEFK

ENNKVTFEMTh

EGDVLHSNKP

ASMVDDINKV

TITTKIKTDA

PKQPLKPKKP

GITKNKKRKN.,

QATFPVQAGE

FMFPDGKTKI

THGOLTNRKT

AAEVLALNQE

FITSQSIDDF

TISATSTEGY

DVTPEDPTIT

\TKVTDENGKD

APYIEQGGIP

WNVKTAFGNE

XQADSYDYLS

'IVTPPAPTPE

LDITDVKVXX

TDEELAPY IE

LTPTNHQAPT

TEPVDLVVVE

NSADYDMNVR

YFLLVTDGVA

ITNQGYEMI.N

TTQLKQ IVKD

VGNITIHYEV

KDIENQEHLD

VTANGTVTQE

NQADLNFCNE

TSTWTQASMV

GHTYTMTITT

DPTITKD lEG

ENGKDVTDNG

QGGI PNQADL

NPVNFCKSAS

DASGS"FSDNF

VNTQLTYDI<S

NTRLDGYLINK

kYWESVESLS

RLAQSTPATA

KENTAIDAAT

LT.NREDSFOW

NNKVTFEMI'JK

GIDVLHSNKPT

DDINKVLDIT

K IKASATDEE

QEH-LDLTNRD

IVTQENNXVT-

NFGNEGDVLH

KGIHLPMTNT

PHVRQAIDEV

QFVSGFGDVR

TNTNDSINEY

SVNSYFDKYK

SLTIANQFE)I

LVSSG.TMNQG

HVKTAFGNET

QADSYDYLSG

VTPPPVDPNI

DVKVTDENGK

rJAPY IEQGG I Q EFKWNVKTA FTIYIqNKDDSY

SNKPTVTPPA

TVNP LYM lAG

VQGLSDQDRV

TYGGTPTAPG

PDPRHPLQVS

TEVGPFVKQE

QSATATDDAG

TIAKEF PEAT

STWTQASMVD

HTYTMTITTK

AKDSJEGQEHL

DVTANGKVTQ

PNQADLNFGN

FGNETSTWTQ

SYLAGHTYTM

PTPEDPKK?E

L IVLIVAI SF p

S.

EF065-3 (SEQ ID NO:247) GITA AAGCAGGAGA TACAGAACGA ATGACCAATA CGGTGAAAGT GAAAGACGAC

AGTCTGGCTG

GAAACGGAAC

TI'CCACATC

GTGATGCTGG

A'ITAATI'CAG

AGCCAATTTG

GGATTGAAAC

ACGTATTTCC

AAGACCAATA

TCAGTGGAAT

GAAATTACTA

AGTTCACTCA

GAGTTGCAAC

TTTACAACCC

GCTTCATTAA

GrGAAATGATG

TACGTAGGAA

ACCCTT'GTALA

ACGATTCCTA

ACAAAAGATA

TGGCATGTCA

GATGACATI'A

GATGTTACAG

AAACAAGCAG

AAAATTAAAA

ATTGTAAACG

CAGTCGAT'TT

TAAGACAAGC

CTTCATATCG

CTGA'ITATGA

TCTCTCGTTT

TCGCTTITAGA

TATTAGTGAC

CCAATGA ITC

ATAGTAATGA

ACCAAGGCTA

ATTCATACTT

AAGGGTCTAG

AATTAAZAACA

CCAIGCCAA

TGCCTGTT-CA

ACATCACGAT

GTAGTGGGAC

AAAATGACAA

TCGAAAATCA

AAACAGCCTT

ATAAAGTGCT

CTAACGGCAC

ACAGCTATGA

CTGACCCAAC

GATATTGGAA

AGTAG T'GTT GA 1TGATGAA

CGGCGGAAAA

TATGAATGTG

TrGGAGACG TT

TACGTACAAT

AGATGGGGTC

AATCAATGAA

CTACCAAGGT

TGAAATGAT

MATAAATAT

CACACCAGAA

AATT'TCAAA

TCAATTTGAT

AATTAACGGA

TCACTACGAA

AATGAATCAA

TGCGCATGCG

AGAACACTTA

TGGCAACGAA

AGATATCATTI

AGTAACACAA

CTATTTAAGT

GGACGAAGAA

GGACAAG CTA

GAAGATGCTA

GTGGTTCAAG

CAATITATrGT

CGCGTCAATA

CGGACGTATG

CAAACACACG

GCTAATACAC

TATCCAGATC

GCAGCAGCAG

AATGCGTA1T

AAAACAGAAG

GA'ITIrALTTA

GATCGTCTGG

ATTCAATCTG

CAAACCAT!Tll

GTCAAAGAAA

GGAACAATTG

TGTGACGTG.A

GATTtAACCA ACCAGTACTr

GATGTGAAAG

GAAAATAACA

GGTCATACGT

TTAGCGCCTI'

CTTTCCCAGT

GTGGTAGrI

GCTTATCTGA

TTCCTGATGG

CGCAATIGAC

GTrGGTACGCC

GAGATTTAAC

G=AGATIGG

CAAGACATCC

AAC~TT=AGC

GGGAAAGTGT

TGGGTCCTTT

CAAGCCAATC

CGCAATCGAC

CGACCGCTAC

CAGCAACTAG

ATACAGCGAT

CTAAGGAATr

CGCCAGAAGA

ATCGTCAAGA

GGACCCAAGC

TCACCGACGA

AAGTAACTTT

ATACAATGAC

ACATTGAACA

TCAAGCGGGT

F1'CAGATAAT

TCAAGACCGC

AAAGACAAAA

TTATGATAAA

AACCGCCCCA

GAATCGAAAA

TTACTTGCAT

TCTTCAAGTC

GTTAAACCAA

TGAATCITA

TGTAAAACAA

TATrGATGAT

ACCAGCAACA

GGACGATGCT

TACAGAAGGT

TGATGCAGCA

TCCAGAAGCG

TCCAACGATT

TAG 'ITCGAT

CAGCATGGTG

AAATGGTAAA

TGAAATGAAC

TATCACCACT

AGGCGGGATT

TABLE I1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

CCCA-ACCAAG

ACCGTAACAC

ITAGATTTAA

GAAACAAGCA

ACTGATGTAA

CAAGAAAATA

AGTGGTCATA

GAATTAGCAC

AACGAAGGTG

GAAGATCCAA

GACCAAGAAT

CAAGCCAGCA

GANGAAAATG

ACTTTTACTA

ATGACTATTA

GAACAAGGCG

CATTCCAACA

GAACCTAAAC

ACGAACCCAG

C-GACTTAAA

CACCGCCAGT

CCAACCGCGA

CTTGGACCCA

AAGTCACAGA

ACAAAGTAAC

CGTACACAAT

CTTATATTGA

ACGMTGCA

CGATTACAAA

TTAAATGGAA

TGGTGGATGA

GCAAAGATGT

TGAACAAAAA

CCACTAAAAT

GGA'I'CCCAA

AGCCAACCGT

AACCGCTAAA

TCAAT'I=GG

CTI'TGGCAAT

TGATCCAAAT

TCAAGAAT'IW

AGCCAGCATG

TGAAAATGGT

TIT=AAATG

GACCATITACT

ACAAGGTGGC

TTCCAACAAA

AGATATCGAA

CGTCAAAACA

CATAATAAA

TACAGATAAT

AGATGACAGC

TAAAACTGAC

CCAAGCCGAC

AACACCGCCT

ACCGAAAAAA

AAAATCAGCA

GAAGGTGACG

ATTGCTAAAG

AAATGGAACG

CTAGATCACA

AAAGATGTTA

AACAANCAAG

ACTAAAATCA

ATTCCCAACC

CCAACCGTAA

GGCCAAGAAC

G.CTTTCGGTA

GTdITTAGACA

GGCATAGTAA

TACTCT'ACT

GCAACGGA1G

TTAAACTTTG

CCACCAACC

CCGTTGACGC

AGTAAAGGAA

TOTTACATTrC

ACGTAGAACG

TCAAAACAGC

TTALATAAAGT

CAGCTAACCG

CNGACAGCTA

AA(3CTAGCGC

AAGCCGACTT

CACCACCTGC

A'FrAGATTT

ACGAAACAAG

TCACAGACGT

CACAAGAAA.A

TAGCTGGTCA

AAGAATTAGC

GCAACGAAGG

CAGAAGACCC

CTACAAATCA

TT

CAACAAACCA

ACAAGAACAT

'TTTCGGTAAC

GTTAGACATC

CAAAGTAACA

TGACTATTTA

AACGGACGAA

ACCAACGCCA

A.ACCAACCGT

CACATCGACC

GAAAGTTNCT

TAACAAAGTA

TACATACACA

GCCTTATATT

TGACGTGTTG

AAAAAAACCT

TCAAGCACCA

EF065-4 (SEQ ID NO:248) a..

a a

AVKAGDTEGM

LADCKRILE G

MLASYRGGKQ

LKLALDTYNQ

VEYSNDYQGA

LQQGSSTPED

NDVPVQINGQ

IPKNDNAHAC

DINKVLDI ID

IKTDATDEEL

DLTNRDQEFK

ENNKVTFEMN

EGDVLHSNKP

ASMVDDINKV

TITTKIKTDA

PKQPLKPKKP

TNTVKVKDDS

QATFPVQAGE

FMFPDGKTKI

THGDLTNRKT

AAEVLALNQE

FITSQSIDDF

TI SATSTEGY

DVTPEDPTIT

VKVTDENGKD

APYIEQGCIP

WNVKTAFCNE

XQADSYDYLS

TVTPPAPTPE

LDITDVKVXX

TDEELAPYIE

LTPTNHQAPT

TEPVDLVVVE

NSADYDMNVR

YFLLVTDGVA

ITNQGYEM~IN

ITQLKQIVKD

VGNITIHYEV

KOIENQEHLD

VTANGTVTQE

NQADLNFGNE

TSTWTQASMV

GHTYTMTITT

DPTITKDIEG

ENGKDVTDNG

QGG IPNQADL

NPVNFGKSAS

DASGSFSIDNF

VNTQLTYD)KS

NTRLDGYLHK

AYWESVESLS

RLAQSTPATA

KENTAIDAAT

LTNREDSFDW

NNKVTFF M

GDVLHSNKPT

DDINKVLDIT

K IKASATDEE

QEHLDLTNRD

IVTQEiWKVT

NFGNEGDVLH

KGIH

PHVRQAIDEV

QFVSGFCGDVR

TNTNDSINE:Y

SVNSYFODKYK

SLTIANQFDI

LVSSGThMNQG

HVKTAPGNET

QADSYOYLSG

VTPPPVPPNI

DVKVTD2ENGK

LAPYIEQGGI

QEFKWNVKTA

FTMNKKDDSY

SNKPTVTPPA

VQGLSDQDRV

TYGGTPTAPG

POPRHPLQVS

TEVGPFVKQE

QSATATDDAG

TIAKEFPEAT

STWTQASMVD

HTYT.MTITTK

AKDVEGQEHL

DVTANCKVTQ

PNQADLNFGN

FGNETSTWTQ

SYLACHTYTM

PTPEOPKKPE

EF066-1 (SEQ I0 NO:249) TAGCGAAAGA AAATAGCCAG ATGGTGATTG TCGCTGGAAC GAAGCGGTTA AAGCAGGAGA AGTCTGGCTG ATTGTAAACG GAAACGGAAC CAGTCGATTT TTTCCACATG TAAGACAAGC GTGATCCTGG CTTCATATCG ATTAAT-TCAC CTGATTATGA AGCCAATTTG TCTCTGG I=I GGATETGAAAC TCGCTTTAGA ACGTATTTCC TA LTAGTCAC AAGACCAATA CCAATGATTC TCAGTGGAAT ATAGTAATGA

GATTAAAATG

AGT'PGTGGGA

TACAGAAGGA

GATATTGGAA

AGTAG7MTTT

GATTGATGAA

CGGCGGAAAA

TATGAATGr

TGGAGACGTT

TACGTACAAT

AGATVCCGTC

AATCAATC-AA

CTACCAAGGT

TTTAAGAAAG

AAT'IrTCAGTC

ATGACCAATA

GGACAAGCTA

CAAGATGCTA

GTCGGTTCAAG

CAATTTATGT

CG-CGTCAATA

CGGACGTATG

CAAACACACG

GCTAATACAC

TATCCAGATC

CAACGAAATT

CCACATT=C

CGGTGAAAGT

CITICCCAGT

GTGCTACTTT

GCT'rATCTGA

TTCCTGATGG

CGCA.ATTCAC

GTGGTACCC

GAGAT'rrAAC

GTI'TACATGG

CAACACATCC

ATTATCGACA

TTAGCTGAA

GAA.AGACG-AC

TCAAGCGGGT

TTCAGATAAT

TCAAGACCGC

AAAGACAAAA

TTATGATAAA

AACCGCCCCA

GAATCGAAAA

TTACTTGCAT

TC'ITCAAGTC

GCAGCAGCAG AAGT'I=AGC GTTAAACCAA 1 149 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

GAAA ITACTA

AGTTCAGTGA

GAGTTGCAAC

TTTACA.ACCC

GCTTCAT'AA

GGAAATGATG

TACGTAGGAA

ACCCTTGTAA

ACGATTCCTA

ACAAAAGATA

TGGCATGTCA

GATGACATTA

GATGTTACAG

AAACAAGCAG

AAAATTAAAA

CCCAACCAAG

ACC.GTAACAC

TTAGATTTAA

GAAACAAGCA

ACTCATGTAA

CAAGAAAATA

AGTGGTCATA

GAATTAGCA.C

AACGAAGGTG

GAAGATCCAA

GACCAAGAAT

CAAGCCAGCA

GANGAAAATG

AC=rTACTA

ATGACTATTA

GAACAAGGCG

CATTCCAACA

GAACCTAAAC

ACGALACCCAG

ACAACAGTAA

TITCGCATAA

ACCAAGGCTA

ATTCATACTT

AAGGGTCTAG

AATTAAA.ACA

CGA'FrGCCAA

TGCCTGTTCA

ACATCACGAT

GTAGTGGGAC

AAAATGACAA

TCGAAAATCA

AAACAGCCTT

ATAAAGTGCT

CTAACGGCAC

ACACCTATGA

CTGACGCAAC

CCGACTTAAA

CACCGCCAGT

CCAACCGCGA

CTTGGACCCA

AAGTCACAGA

ACAAAGTAAC

CGTACACAAT

CTTATA ITGA

ACGTGTTGCA

CGATTACAAA

TTAAATGGAA

TGGTGGATGA

GCAAAGATGT

TGAACAAAAA

CCACTAAALAT

GGATTCCCAA

AGCCAACCGT

AACCGCTAAA

TCAArT-rcc

ATCCACTTTA

CAAAAAATAA

TGAAATGATT AATGCGTATT

TGATAAATAT

CACACCAGAA

AATTGTCAAA

TCAATI'TGAT

AATTAACGGA

TCACTACGAA

AATGAATCAA

TGCGCATGCG

AGAACACTTA

TGGCAACGAA

AGATATCAT'

AGTAACACAA

CTATTTAAGT

GGACGAAGAA

CTTTGGCAAT

TCATCCAAAT

TCAAGAA=T

AGCCAGCATG

TGAAAATGGT

TTTTGAAATG

GACCATTACT

ACAAGGTGGC

TTCCAACAAA

AGATATCGAA

CCTCAAAACA

CATTAATAAA

TACAGATAAT

AGATGACAGC

TAAAACTGAC

CCA.AGCCGAC

AACACCGCCT

ACCGAAAAAA

AAAATCAGCA

CATCATCGCA

AAAAAGAAAA

AAAACAGAAG

GATTT1TATIA

CATCGTCTGG

ATTCAATCTG

CAAACCATTT

GTCAAAGAAA

GGAACAATTG

TGTGACGTGA

GATTTA.ACCA

ACCAGTACTT

GAtGTGAAAG

GAAAATAACA

GCTCATACCT

TTAGCGCCTT

CAAGGTGACG

ATTGCTAAAG

AAATGGAACG

GTAGATGACA

AAAGATGITA

AACAANCAAG

ACTAAAATCA

ATTCCCAACC

CCAACCGTAA

GGCCAAGAAC

GCTTCGGTA

GTGMACACA

GGCATACTAA

TACTCTTACT

GCAACGGATG

TTAAACT=T

GCACCAACGC

CCGTTGACGC

AGTAAAGGAA

GGTTAA=~

AATTAG

GGGAAAGTGT

TGGGTCCTTT

CAAGCCAATC

CGCAATCGAC

CCACCOCTAC

CAGCAACTAG

ATACA.GCGAT.

CTAACGAATT

TGAATCTTTA

TGTAAAACAA

TATTGATGAT

ACCAGCAACA

GGACGATGCT

TACAGAAGGT

TGATGCAGCA

TCCAGAAGCG

CGCCAGAAGA TCCAACGATT ATCGTG.AAGA TAGTI'TCGAT GGACCCAAGC CAGCATGGTG TCACCGACGA AAATGGTAAA AAGTAACTTT TGAAATGAAC ATACAATGAC TATCACCACT ACATTGAACA AGGCGGGATT TGTTACATrC CAACAAACCA ACGTAGAAGG ACAAGAACAT TCAAAACAGC TTTCGGTAAC TTAATAAAGT GTTAGACATC CAGCTAACGG CAALAGTAACA CNGACAGCTA TGACTArI-rA AAGCTAGCGC AACGGACGAA AAGCCGACTT GAACTTTGGC CACCACCTGC ACCAACGCCA ATTTAGATTT AACCAACCGT ACGAAACAAG CACATGGACC TCACAGACGT GAAAGF1'NCT CACAAGAAAA TAACAAAGTA TAGCTGGTCA TACATACACA AAGAATTAGC CCCTTATATT GCA.ACGAAGG TGACGTCTTG CAGA.AGACCC AAAAAAACCT CTACAAATCA TCAAGCACCA TTCATIACC AATGACTAAT TCCTTATAGT GGCTATTAGC a.

a a a a a a a a a.

EF066-2 (SEQ ID NO:250) MF KKATKLLSTM VIVAGTVVCN FSPTLALAEE AVKAGDTEGM TNTVKVKDDS

LADCKRILEG

MLASYRGCKQ

LKLALDTYNQ

VEYSNDYQGA

LQQGSSTPED

NDVPVQ INGQ I PKND1NAHAC DINKVLDI ID

IKTDATDEEL

DLTNRDQEFK

ENNKVTFEM

EGDVLHSNKP

ASMVDDINKV

TITTKIKTDA

QATFPVQAGE

FMFPDGKTK I

THGDLTNRKT

AAEVLALNQE

FITSQSI-DDF

TISATSTEGY

DVTPEDPTIT

VKVTDENGKD

APYIEQGGIP

WNV'KTAFGNE

XQADSYDYLS

TVTPPAPTPE

LDITDVKVXX

TDEELAPY IE

TEPVDLVVVE

NSADYDMNVR

YFLLVTDGVA

ITNQGYEMIN

TTQLKQIVKD

VGNITIHYEV

KDIENQEHLD

VTANGTVTQE

NQADLNFGNE

TSTWTQASMV

GHTYTMTITT

DPTITKDIEG

ENGKDVTDNG

QGGIPNQADL

DASGSFSDNF

VNTQLTYDKS

NTRLDGYLHK

AYWESVESLS

RLAQSTPATA

KENTAI DAAT

LTNREDSFDW

NNKVTFEM

GDVLHSNKPT

DE)INKVLDIT

KIKASATD'EE

QEHLDL'rNRD

IVTQENNXVT

NPGNEGDVLH

PHVRQAIDEV

QFVSGFGDVR

TNTNDSINEY

SVNSYFDKYK

SLTIANQFDI

LVSSGTMNQG

HVKTAFGNET

QADSYDYLSG

VTPPPVDPNI

DVKV'rDENGX

LAPYIEQGGI

QEFKWNVKTA

FTMNXKKDSY

SNKPTIVTPPA

VQGLSDQDRV

IYGGTPTAPG

PDPRIIPLQVS

TE'JGPFVKQE

QSATATDDAG

TIAKEFPE-AT

S1 ATQASMVD

HTYTMTITTK

AKDVEGQEHL

DVTANGKVTQ

PNQADLNFGN

FGNETSTqhTQ

SYLAGHTYTM

PTPEOPKCPE

150 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

PKQPLKPKKP LTPTNHQAPT NPVNFGKSAS KGIHLPMTNT TVNPLYI4IAG LIVL-7VAISF C ITKNKKRKN EF066-3 (SEQ ID NO:251) GGTTA AAGCAGGAGA TACAGAAGGA ATGACCAATA CGGTGAAAGT GAA.AGACGAC

AGTCTGGCTG

GAAACGGAAC

TTTCCACATG

GTGATGCTG

A'ITA-ATTCAG

AGCCAATTTG

GGATTGAAAC

ACGTAICC

A.AGACCAATA

TCAGTGGAAT

GAAATTACTA

AGTTCAGTGA

GAGTTGCAAC

TTTACAACCC

GCTTCATTAA

GGAAATGATG

TrACGTAGGAA

ACCCTICTAA

ACGATTCCTA

ACAA.AAGATA

TCGCATGTCA

GATGACATTA

ATTGTAAACG

CAGTCGATTT

TAAGACAAGC

CTTCATATCG

CTCA ETATGA

TCTCTIGGTTT

TCGCTTTAGA

TATTACTGAC

CCAATGATTC

ATAGTAATGA

ACCAAGGCTA

A ITCATACTT AkAGGGTCTAG

AATTAAAACA

CGATTGCCAA

TGCCTGTTCA

ACATCACGAT

GTAGTCG-AC

AAAATGACAA

TCGAAAATCA

AAACAGCCTT

ATAAAGTGCT

GATATTGGAA

AGTAC ITCTT

GATTGATGAA

CGGCGGAAAA

TATGAATGTG

TGGAGACGTT

TACGTACAAT

AGATCGGGTC

AATCAATGAA

CTACCAAGGT

TGAAATGAT

TGATAAATAT

CACACCAGAA

AATTGTCAAA

TCAATTTCAT

AATTAACGGA

TCACTACGAA

AATGAATCAA

TCCATGCG

AGAACACT'A

TGGCAACGAA

AGATATCATT

GGACAAGCTA

CAAGATC TA

GTGGTTCAAG

CAA'IIA-TGT

CGCGTCAATA

CGtCACGTATG

CAAACACACG

GCTAATACAC

TATCCAGATC

GCAGCAGCAG

AATGCGTATT

AAAACAGAAG

GATFITATTA

GATCGTCGG

ATTCAATCTG

CAAACCATT

GTCAAAGAAA

GGAACAA=P

TGTGACGTGA

GATT-1AACCA

ACCAGTACT

GATGTGAAAG

CTTTCCCAGT

CTCGTAGT

GCTTATCTGA

TTCCTGATGG

CCPATTGAC

GTGGTACGCCC

GAGA=-TAAC

G'ITAGATGG

CA.AGACATCC

AAGTTTTAGC

GGGAAAGTGT

TGGGTCC=T

CAAGCCALATC

CGCAATCGAC

CGACCGCTAC

CAGCAACTAG

ATACAGCGAT

CTAAGGAATT

CGCCAGAAGA

ATCGTGAAGA

GGACCCAAGC

TCA

TCAAGCGGGT

TTCAGATAAT

TCAAGACCGC

AA.AGACAAAA

TTATGATAAA

AACCGCCCCA

GAATCGAAAA

TTACTTGCAT

TCTTCAAGTC

GTTAAACCAA

TGAATCTTITA

TGTAAAACA-A

TATTGATGAT

ACCAGCAACA

GGACGATGCT

TACAGAAGGT

TGATGCAGCA

TCCAGAAC

TCCAACGATT

TAGTTCGAT

CAGCATGGTG

S

S..

*5 5

C.

EF066-4 (SEQ ID. NO:252)

AVKAGDTEGM

LADCKRILEG

MLASYRGGKQ

LKLALDTYNQ

VEYSNDYQGA

LQQGSSTPED

1NDVPVQINGQ I PKNDNAHAC

TNTVKVKDDS

QATFPVQAGE

FMFPDGKTKI

THGDLTNRKT

AAEVLALNQE

FITSQSIDDF

TI SATSTEGY

DVTPEDPTIT

T:EPVDLVVVE

NSADYDMNVR

YFLLVTDGVA

ITNQGYEMIN

TTQLKQIVKD

VGNITIHYEV

KDIENQEHLD

DASGSFSDNF

VNTQLTYDKS

NTRLDGYLHK

AYWESVESLS

RLAQSTPATA

K=NAIDAAT

LTNREDSFDW

PHVRQAIDEV

QFVSGFGDVR

TNTNDS INEY

SVNSYFDKYK

SLTIANQFDI

LVSSGTMNQG

HVKTAFGNET

VQGLSDQDRV

TYGGTPTAPG

POPRHPLQVS

TEVGPFVKQE

QSATATDDAG

TIAKEFPEAT

STWTQASMVD

DIrxCVLDIID VKVT EF067-1 (SEQ ID NO:253)

TAGCGAAAGA

ATGGTGATTG

GAAGCGGITA

AGTCTGGCTC

GAAACGGAAC

T ITCCACATG

GTGATGCTGG

ATTAATTCAG

AGCCAATTC

GGATTGAA-AC

ACGTATTTCC

AAATAGGGAG

TCGCTGGAAC

AAGCAGGAGA

AITGTAAACG

CAGTCGATTT

TAAGACAAGC

CTTCATATCG

CTGA ITATGA TCTCTGGTrr

TCGCTTTAGA

TATTAGTGAC

GATI'AAAATG

AGTI'GTGGGA

TACAGAACCA

GATATTGGAA

AGTAGTTGTT

GATTGATGAA

CGCCGGAAAA

TATGAATGTG

TGGAGACGTT

TACGTACAAT

AGAT'GGGGTC

TTAAAAAG

AATTTCAGTC

ATGACCAATA

GCACAAGCTA

GAAGATGCTA

GTGG ITCAAG CAA TTATCT

CGCGTCA-ATA

CGGACGTATG

CAAACACACG

GCTAATACAC

CAACGAAATT

CCACAMTGC

CGGTGAAAGT

CTTTCCCAGT

GTGGTAGTTT

GCTTATCTGA

TCCTATGG

CGCAA'TMAC

GTGGTACGCC

GAGAMAAC

G ITTAGATGC

ATTATCGACA

TTTAGCTGAA

GAAAGACGAC

TCAAGCGGGT

TTCAGATAAT

TCAAGACCGC

AAAGAcAAAA

TTATGATAAA

AACCGCCCCA

GAATCGAAAA

'FTACTTGCAT

TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

AAGACCAATA

TCACGTGGAAT

GAAA'ITACTA

AGTTCAGTGA

CAGTTGCAAC

TTTACAACCC

GCTTCATTAA

GGAAATCATC

TACGTAGGAA

AC CC TTGTAA

ACGATTCCTA

ACAAAAGATA

TGGCATGTCA

GATGACATTA

GATG'ITACAG

AAACAAGCAG

AAAATTAAAA

CCCAACCAAG

ACCGTAACAC

TTAGATTTAA

GAAACAAGCA

ACTGATGTAA

CAAGAAAATA

AGTGGTCATA

GALATTAGCAC

AACGAAGGTG

GAAGATCCAA

GACCAAGAAT

CAAGCCAGCA

GANGAAAATG

ACTTTTACTA

ATGACTATTA

GAACAAGGCG

CATTCCAACA

GAACCTAAAC

ACGAACCCAG

ACAACAGTAA

TTTCCCATAA

CCAATGATTC

ATACTAATGA

ACCAAGGCTA

ATTCATAC T

AAGGGTCTAG

AAITAAAACA

CGATTGCCAA

TGC^CTGTTCA

ACATCACGAT

GTAGTGGGAC

AAAATGACAA

TCGAAAATCA

AAACAGCCTT

ATAAAGTGCT

CTAACGGCAC

ACAGCTATGA

CTGACGCAAC

CCGACTTAAA

CACCGCCAGT

CCAACCGCGA

CTI'GGACCCA

AAGTCACAGA

ACAALAGTAAC

CGTACACAAT

CTTATATTGA

ACGTG'FrCCA

CGATTACAAA

TTAAATCGAA

TGGTGGATGA

GCAAAGATGT

TGAACAAAAA

CCACTAAAAT

GGATTCCCAA

AGCCAACCGT

AACCGCTAAA

TCAATrTTGG

ATCCACTTTA

CAAAAAATAA

AATCAATGAA

CTACCAAGOT

TGAAATGATT

TrGATAAATAT

CACACCAGAA

AATTGTCAAA

TCAATTAT

AATTA.ACGGA

TC-ACTACGAA

AATGA.ATCAA

TGCGCATGCG

AGAACACTTA

TGGCA.ACGAA

AGATATCATT

AGTAACACAA

CTATIAAGT

GGACGAAGAA

CTTTGGCAAT

TGATCCAAAT

TCAAGAATTT

AGCCAGCATG

TGAA.AATGGT

TTTTGAAATG

GACCATI'ACT

ACAAGGTGGC

TTCCAACAAA

AGATATCGAA

CGTCAAAACA

CATTAATAAA

TACAGATAAT

AGATGACAGC

TAAAACTGAC

CCAAGCCGAC

AACACCGCCT

ACCGAAAAAA

AAAATCAGCA

CATCATCGCA

AAAAAGAAAA

TATCCAGATC CAAGACATCC TCTTCAAGTC CCAGCACCAG AAGT='AGC GTTAAACCAA AATGCGTAIT GGGAAAGTCT TCAATCTTTA AAAACAGAAG TGGGTCCTTT TGTAAAACAA GATTTTAT1'A CAAGCCAATC TATTGATGAT GATCGTCTCG CGCA.ATCGAC ACCAGCAACA ATTCAATCTG CGACCGCTAC GGACGATGCT

CAAACCATI

T CAGCAACTAG TACAGAAGGT GTCAAAGAAA ATACAGCGAT TGATGCACCA GGAACAATTG CTAACCAATT TCCAGAAGCG TGTGACGTGA CGCCAGAAGA TCCAACGATT GATTAACCA ATCGMIAAGA TAGTFTCGAT ACCAGTACTI' GGACCCAAGC CAGCATGGTG GATGTG-AAAG TCACCCACGA AAATGGTAAA GAAAATA.ACA AAGTAACTTT TGAAATGAAC GGTCATACGT ATACAATGAC TATCACCACT TTAGCGCCTT ACATTGAACA AGGCGGGAhTT CAAGGTGACC TCTTACATTC CAACAAACCA ATTGCTAAAG ACGTAGAAGG ACAAGAACAT AAATGGA.ACG TCAAAACAGC TTCGGTAAC GTAGATGACA TTAATAAAGT GTTAGACATC AAAGATGTTA CAGCTAACGG CAAAGTAACA AACAANCAAG CNGACAGCTA TGACTATTTA ACTAAAATCA AAGCTAGCGC AACGGACGPA ATTCCCAACC AAGCCCACTT GAACTGG-C CCAACCGTAA CACCACCTCC ACCAkACGCCA GGCCAAGAAC ATT1TAGA=T AACCAACCGT GC=rCGGTA ACGAAACAAG CACATGGACC GTGTTAGACA TCACAGACGT GAAAGTTNCT GGCATAGTAA CACAAGAAAA TAACAAAGTA TACTCTTACT TACCTCCTCA TACATACACA GCAACGGATG AAGAATTACC GCCTTATAT TTAAACTT7G GCAACGAAGG TGACGTGTTG GCACCAACGC CAGAAGACCC AAAAAAACCT CCGTI'GACGC CTACAAATCA TCAACCACCA AGTAAAGGAA TTCATI'TACC AATGACTAAT GGT EAATTG TCCTTATAGT GGCTAITAGC

AATTAG

*00

S.

S

.00.

5555 55..

S

55..

0

S'S.

S5 S

S.

.5 EF067-2 (SEQ ID NO:254) M~F KKATKLLSTM VIVAGTVVGN FSPTLALAEE AVKAGDTEGM TNTVKVKDDS LADCKRItLEG

MLASYRGGKQ

LKLALDTYNQ

VEYSNDYQGA

LQQGSSTPED

NDVPVQINGQ

I PKNDNAHAC DINKVLDI ID

IKTDATDEEL

DLTNRDQEFK

ENNKVTFEM

EGDVLHSNXP

QATFPVQAGE

FMFPDGKTKI

THGDLTNRKT

AAEVL.ALNQE

FITSQS IDOF

TISATSTEGY

DVTPEDPTIT

VKVTDENGKD

APYIEQGGI P

WNVKTAFGNE-

XQADSYDYLS

TVTPPAPTPE

TEPVDLVVVE

NSADYDMNVR

YFLLVTDGVA

ITNQGYEMIN

TTQLKQIVKD

VGNITIHYEV

KDIENQEHLD

VTANGTVTQE

NQADLNFGNE

TSTWTQASMV

GHTYTMTITTI

DPT ITKD 120

DASGSFSDNF

VNTQLTYDKS

NTRLDGYLHK

AYWESVESLS

RLAQSTPATA

KENTAI DAAT

LTNREDSFDW

NNKVTFEMNKM

GDVLHSNKPT

DDINKVLDIT

KIKASATDEE

QEHLDLTNRD

PHVRQAIDEV

QFVSGFGDVR

TNTNDSINEY

SVNSYFDKYK

SLTLANQFDI

LVSSGTMNQG

HVKTAFGNET

QADSYDYLSG

VTPPPVDPNI

DVKVTDENGK

LAPYIEQGGI

QEFKVINVKTA

VQGLSDQDRV

TYGGTPTAPG

PDPRHPLQVS

TVEVGPFVI<QE

QSATATDDAG

TIAKEFPEAT

STWTQASMVD

HTYTMTITTK

AKDVEGQEHL

DVTANGKVTQ

PNQAOLINFGN

FGNETST'JTQ

152 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

ASMVDDINKV LDITDVKVXX ENGKDVTDNG IVTQENNKVT FTMKKDDSY SYLAGHTYTN TITTKIKTDA TDE-ELAPYIE QGGIPNQADL NFGNEGDVLHi SNK(PTVT-PPA PTPEDPKKPE PKQPLKPKKP LTPTNH-QAPT NPVNFGKSAS KGIHLPMTNT TVNPLYMIAG LIVLIVAISF C ITKNKKRKN EF067-3 (SEQ ID NO:255) GCT AGATATCATT GATCTGAAAG TCACCGACGA AAATCCTAAA

GATGTTACAG

AAACAAGCAC

AAAATTAAAA

CCCAACCAAG

ACCGTAACAC

TTAGATTTAA

GAAACAAGCA

ACTGATGTAA

CAAGAAAATA

AGTGGTCATA

GAATTAGCAC

AACGAAGGTC

GAAGATCCAA

GACCAAGAAT

CAACCCAGCA

GANGAAAAT G

ACTTTTACTA

ATGACTATTA

GAACAAGC

CATTCCAACA

CAACCTAAAC

ACGAACCCAC

CTAACGGCAC

ACAGCTATGA

CTGACGCA.AC

CCGACTTAAA

CACCGCCACT

CCAACCCA

CTTGGACCCA

AAGTCACAGA

ACAA.AGTAAC

CGTACACAAT

CTTATATTGA

ACGTGTTGCA

CGATTACAAA

TTAAATGGAA

TCCTGGATGA

GCAAAGATGT

TGAACAAAAA

CCACTAAAAT

GGATI'CCCAA

AGCCAACCGT

AACCGCTAAA

TCAATTTTC-G

AGTAACACAA

CTATTTAAGT

GGACGAAGAA

CTTTGGCAAT

TGATCCAAAT

TCAAGAATT

AGCCAGCATG

TGA.AAATGGT

TTCAAATC

GACCA PTACT

ACAAGGTGGC

TTCCAACAAA

AGATATCGAA

CGTCAAAACA

CATIAATAAA

TACAGATAAT

AGATGACAGC

TAAAACTGAC

CCAACCCGAC

AACACCGCCT

ACCGAAAAAA

A.AAATCAGCA

GAAAATAACA

GGTCATACGT

TTAGCGCCTT

GAAGGTGACG

ATI'GCTAAAG

AAATGGAACG

GTAGATGACA

AAAGATGTTA

AACAANCAAG

ACTAAAATCA

ATTCCCAACC

CCAACCGTAA

GGCCAAGAAC

GC=ICGGTA

6TGTTAGACA

GGCATAGTAA

TACTCTTACT

CCAACGGATG

TTAAAC=G~

GCACCA.ACC

CCGTTGACGC

AGTAAAGGAA

AAGTAAC'TTT

ATACAAPIGAC

ACATTCAACA

TGTTACATTC

ACGTAGAAGG

TCAAACAGC

TAA.TAAAGT

CAGCTAACGG

CNGACAGCTA

AAGCTAGCC

AAGCCGACTT

CACCACCTGC

ATTTACATTT

ACGAA.ACAAG

TCACAGACGT

CACA.AGAAAA

TAGCTGGTCA

AAGAATTAGC

GC'AACGAAGG

CAGAAGACCC

CTACAAATCA

TT

TGAAATGAAC

TATCACCACT

AGGCGGGATT

CAACAAACCA

ACA.AGAACAT

=TCGGTAAC

GTTAGACATC

CAAAGTAACA

TGACTAT=A

AACGGACGAA

GAAC1TGGC

ACCAACGCCA

AACCAACCCT

CACATGGACC

GAAAGTTNCT

TAACAAAGTA

TACATACACA

GCCTTATATT

TGACGTGTTG

AAAAAAACCT

TCA.AGCACCA

00 00 S S0 0e 0 0

S.

00 0

S.

S.

S S 000 S

SS

S S @06

S

0055 Se

S

*050 0

S.

*550

S

0 0000 0@*0 0 0555 S. S 05 EF067-4 (SEQ ID NO:256) VLDI ID VKVTDENGKO VTANGTVTQE NNKVTFEMINY QADSYDYLSG HTYTMTITI'K

IKTDATDEEL

DL.TNRDQEFK

ENNKVTFEMN

EGDVLH-SNK(P

ASMVDDIN(V

TITTKIKTDA

PKQPLKPKKP

APYIEQGGIP

WNVKTAFGNE

XQADSYDYLS

TVTPPAPTPE

LDITDVKVXX

TDEEL.APYIE

LTPTNHQAPT

NQADLNFGNE

TS IWTQASMV

GHTYTMTITT

DPTITKDIEG

ENGKDVTDNG

QGGIPNQADL

NPVNFGKSAS

GDVLHSNKPT

DDINKVLDIT

KIKASATDEE

QEHLDLTNRD

IVTQENNKVT

NFCNEGDVLH

KG Dl

VTPPPVDPNI

DVKVTDENGK

LAPYIEQGGI

QEFKWNVKTA

FTMNKKDDSY

SNKPTVTPPA

AKDVEGQEHL

DVTANGKVTQ

PNQADLNFGN

FGNETSTWTQ

SYLAGHTYTM

PTPEDPKKPE

EF068-1 (SEQ ID NO:2S7)

TAGGGGAAGC

ATGAAAAAGA

CCCAAGATGC

GTAAGTATAG

CTATTATCAA

CCCCCAA.ACC

TCCGCTGTGT

AATGTAGCTG

TAATGATCT

AAAITGTTGA

TTAATTTAGC

CTGTTACCTC

ATGTTACGTC

AAAATCAACC

FIAGTGGACA

CAGCAGGCAG

GGTATTTATC

GGATTTTAAT

AATATCAAGT

TGGCACAATC

AAATAATGAC

AGTTAATTTC

AAAACAAGCG

CGCAGCAATC

GTTTATTTTA

CGGAAAAGTC

GGTTIATTAT

AGTGCATCAG

AGTGGCACTT

ACGGITPTCT

GTGTTAGTGG

AATACCAATG

AAGAAAAGAG

AGCATAAAAA

'ITACGTCA'Fr CACGCTC IT

CAACGAGTAA

GTGGCGCTT

'TCCTCCTGA

TCACGATTGA

GGACGATCAG

ATGGACAAAA

AGCAATCCCT

GGATATCGAA

TCGTTGGACA

AGCAGATGCT

CTTAAGAGGA

TCTTTCAAAA

153 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.f[accalis Genes.

GTTACTTTTT

ATTACCAGTG

CGTCAATTGG

GAAACGTTAG

GT=TAGCCC

GAGGCAAAAG

CVI'CCTGTCA

GGTGGTTCAG

TACCAACTA

GGAACAGTCG

TCCAACATTT

GTAACAGGTA

GTTGAAATCC

GCGITTACAG

GCGAAAAACG

GCAACCGTAA

GTTAAAGGAA

ATAGGCACAG

GCAGGTGCCA

CCAACAACGT

GTGACAGGTA

GTTGAGATCC

GCATTTACAG

GCGAAAAACG

AATACGCCCG

GAGGTCAAAG

GTCCTTGGTA

ACAGCAACAG

CAACCAGCAA

GGCAACTCTG

GTTCGTGATC

ACTGTAACGC

GATAACGCG

ACAGCACCAA

ACCGCTGATC

GGGACTGCCG

AATGAAACAT

TTCCAAACAC

ACAACACAAG

GCAACAGACG

ACGTTAGCI'

ACTGGACTTG

ATTGGTGACA

AATACCACCA

GATCAAGGAA

ACAGTTGTTC.

GAAGATGCAA

TATCAAGTAA

TTAGTTATTG

GGCACAGCAA

AGTAATCCGA

GTTGACAAAA

GGTACAACAG

ACTGATGGCA

TGACTOCCGT

GGGCGTTAGG

AATTAGTTAA

CAGCTCACGG

AAAATG=T

CTACCAGTAT

AAGGCACGGT

GCGTAAATGA

CCGTTTCAAC

TTCAAACAGA

A=TTGCTGC

ATTCAACAGC

CAAATGCAGG

TTACCGTTCC

CCAGCGGNAC

CCGCACCAAC

CTGCCGATGC

GTACCGCTGA

ATGAAACGTT

TCCAAACACC

A'ITCAACAGC

GAAATGCAGG

TTACCATTCC

CCAGCGGTAC

TGGCGACGCC

GGACTG.CTGA

CTGCAACAAC

CAAATCAAAC

CGGCGACAAC

GTTCGGGTTA

CATCTGGGGC

TACCAACGGG

GAAATGAAAG

CTGTAACAGG

CGAATGCTAC

ATGGGACTOG

TGACAGCGTT

CAGCAGMA

GATATCAAGT

GAACAGTTF

CAGGAAAAGC

AACTCAACC

TTACTGGAGA

TTGAAGTACG

ACTTTACTOT

GAAAAGACCG

CCGTAGCAGC

CCGGCACGGC

CTACCGGAAC

CACCTAACGA

CTCrrCAA

TCACTGGTAG

TTIGAGGTGCG

AATACACAGT

rTTAATGCA

GAATTAACT

TAACATTGAA

CTCATACATT

AAACATCTT-A

CCCAAGTAAT

AAACGTGGCT

GTTAGTGGAT

ACCTICAAAAT

TCT'ITAGAC

AGGCACTACT

AGGTTACGAA

AGGCACCGTA

CGCAGGTGAA

AGAAAGNACG

AATCACAGGA

CAATGCCACG

TGGGACAGGA

AACCGCCGTA

AGCATGA-A

AGGITACGAA

AGGTGC-GTG

CCCAGGTGAA

AGAAAGTACG

AATTGTTGAG

ACTTGCACC

TGGAACTGAC

GCTG-AGCGTT

ACCAGCTGAT

TGAAATTACA

AGTCATTGGT

AACGACCAAT

TCAACCGACT

AGTAACAGGT

CATCGAAATT

T1?CCTTTGCT

AGCCA.AAGAT

AGTAGTIGGCA

GACAGGTACC

AGGCACCGCA

AACAGCTAAA

AACTACAGCT

TTCAACAACT

GAACCCAGAT

GGACCTTCCA

TGACGGCAAT

ACCAACTiGTG

AGAGCCAAAT

GACTGATGGT

AGCCTTAACT

AACACCTGCT

CACGACAAAC

TGACGCCGAT

GAC=TAGAG

GCCAATGAr12

GGTGTTGATA

A.ACTTAGGTG

A.GTGCACCGA

CAAGATTTGA

CTTGTCGCCG

GTTTCACCTG

GCTTCTTTAC

TTATCCAATA

GTTAATTTAT

ACTCA.AGTAA

GTTAAAGGAA

ATAGGCACAG

GCAGGCGCCA

CCAACAACGT

GTGACAGGTA

GTTGAA.ATCC

GCGTTTACAG

GCGAAAAACG

CCAACCGTAA

GTTAAAGGAA

ATAGGTACAG

GCAGGTGCGA

CCAACAACGT

ACTGTAACAG

ACCATTGAGG

GGAAAATATA

GTAGCGAAAAA

GTCACTGCAC

GGAACAGCAG

ACAGGTACCT

CCTGGGGATA

GAAGTCCTTrG

AATTCAG=~

CGTGATGCAG

GTGAACCTTC

CCTGCTGGCA

CCGCCAAGTG

GCTGAACTTG

ACAACTGGAC

CAAACAGTGA

ATGACACCCG

GGTTATGAAA

GGAACAA ITA

GCGGGAGCCG

GAAAGTCAAC

ACGACTGTTA

GTCACCATTG

GCTGCGCAT

GCCAT JXCGA GATCCAGA G

CGCTATCAAG

CCCACAGTCC

CCAGGGAAGG

TAACCAATCT

TTATTTGAC

CTGCTTCATT

TTAGTGATG

ATGCCGGCAGT

CACCTATAAA

CTTTGCCTTTr

TAGGCACAAC

ATTTAGATCC

TAGCAACAGC

CCGCACCA-AC

CTGCCGATGC

GTACCGCTGA

ATGAP ACGTT

TCCAAACNCC

A/FTCAACGGC

GAAATGCAGG

TTACCGTTCC

CC:AGCGGCAC

CCCCACCAAC

CTGCCGATGC

GTACTGCTGA

ATGAAACOTT

TCCAAACGCC

GTAGTACAAC

TTCGCGATGC

CACTGACTTT

ACGCTAGTGG

CAACAGMITA

ACCCTAACAC

CTGATGCGALA

CGTTAACAGT

MICCTGCTGA

CTGGTTATCA

ATGGGAACGT

CAGCTGOGAC

ATACAAGTAC

TCGACAAAGT

GCACCACCAT

CGACTGGCCA

ATGTAG=ITC

CTGATGTI'AC

TCACTGGGAC

TTGGTACAAC

CTAATCCTGG

CAACGGAAGT

CAGGAACAAC

AGATCACAA

TACAATCAC

AAGATGCTGC

CACCAGTCGC

TAGTAGGAGC

TTGGCATGGC

CCTCAGCTAA

GATTACTCAA

GGAAGTGAAT

TACAGCTCCG

TTACGGTTA

TCAAGCTTTG

TGCAGCCTTG

ATTAGCGGTT

CACGGTTACT

TCGTTTTGTA

AGACGGTGTA

AATCACAGGA

CAATGCCACG

TGGGACAGGA

AACCGCCCTA

AGCGGATGA.A

AGGTTACGAA

AGGCACCGTA

CGCAGGTGAA

AGAAAGTACG

AATCACAGGA

CAATGCCACG

TGGGACAGGG

AACCGCCGTA

AGCGGATCCT

AAAAGGCTAT

AGCTGGCACG

AGATTCAGGA

CACGGAAAGT

TAACATCACA

AACAATCGAA

TGGTGATTTT

GATTGGAAAG

TGCCACGGTT

GGTGACAGGC

GATTGCAACA

GGCAAATGCG

ACCGACA-ACC

TACTGGGAAT

TGAAGTTCGT

ATATACTGTG

TAAAAATGAT

CACACCAACA

GGCGGACCCT

GACAACGGAT

TGATACATTA

GACGGTCCCT

TGCCACTGGG

TGAAGCAGGT

TCTTCCGACG

TGGGAAAGAA

GACACCTACT

AGCAGAAGTT

AACTACTGCA

CGAAACAATA

TABLE 1. Nucleotide and Amino Acid Seqeuences of E. *faecalij Genes.

ACTGTCGTAG

GTCGACTTAG

ATCACTGGAA

ATTGCTCCTA

GTGACACCAG

CCAGCTACAG

CAACGAAACA

CAATTTTACA

TTCTCCGTTC

CGAAAAATC

CCACACCAAC

CGGC--GAGCC

CAACTGCTAA

GAGAAACGAT

CCGTTATTCC

AAGCCAATG

ATAGCAGTGA

ATAITGCAGC

AACAGGAAA

CATTGATTCT

AAAA.ACCACT

CGAAACCGGC

TACGATTA2T AGCGGATG TT

CTATACAGTC

ACAAT~A L7G AGGGT1'AGCA ACACATACAC A.AGGAAATGT GAGTCCTAAA

GGAGAACCAG

GCCGGCTACC

CTATTAAGAA

TTAACAGCCG

GGACATTACA

GGCAATGGTA

GCAACTGGCG

GGTTTAGGCA

ACTGGCTACC

GGCTACTTAC

CC 'FTGTCG

AGATITAAA.AT

TCATCAAAGG

GTCTGATTGC

GACAAGCGAC

GTACGGCTAC

ACACTGGCGG

GGAATAATGG

CAACAGGTTC

ACCCTAAACT

CTAGCACACG

CACTCCTTC

TGCGGCACCA

AACAGCTGAA

TGTAGGTGCC

TGCTCAACA-A

AACCTTCATG

AAATAACGGC

GAATGTCA

TGGCTTAGGT

AAGTACCATC

TGAAAAAGAG

GAGCATGGGA

GAAAGTCAGC

ATI'ACCGGAA

ATTrGATGTCC

CAATATACGG

AGCAAAGATG

GTI ITAGCGG

ACTGGAACTG

GCAAGTGGCA

ACAGAAAAAG

ACCACAF1'A

ACTGTTTCTT

CECAAACCGAA

ACCGATGCTG

AGTTTACTTC

ACGCCAGCCG

AATACAGGCG

AACACAGGTT

TCACTAGGCA

AGTTATGGCA

TCTTCAGCCG

ATCATCAAAC

CAGCTACACC

AITCTAGTAA

GTGACGCAGA

TGACTCTACC

CCAGGTAA

CGCCAACTAT

CTGATCCAAA

ATACAACTAC

AAACGTTAAC

TGACGCCAC

CAGTTTTACG

TCA'ITCAAAT

AAGGCAACTT

CGACA:OCTAC

ACCAACGAA

GCAATACAGG

CAAATCCAAA

ATGGCCTCGG

CTGGAAATCA

TCACAACAAG

GCAAACGTAA

AACTACACCA

AGGTTACGAA

CGGAACCATC

AGCTGGCGTA

TGAAAGTICAA

TACGAAGGTT

TrGTCACGGTT

LGGAGGTACC

CGCACTAACC

CGATATTACG

A.ACGTCTAAA

TAGTAACCGA

CGCTATCCA

CGAMGCGCA

TCCMGAGGA

AAACAATGCC

TGGAGGCTCT

TACAAATCCT

CGGGAAAACA

TTTTTTGGC

AAACTAG

o 00% .0.

*.0 0 *0.

1.: EF068-2 (SEQ ID NO:258) M KKKIVEDFNR KSQHKKWTKR KMLNLAISSG LLTSLAIPV S IAVTSGTIS

AVFSGQKQAV

TSGALGNLTG

LAQNWVSNILQ

GSGVNELVDA

NIYFAAGTTS

FTVTVPAGEA

KGTADANATV

TTFQTPADEA

FTVTI PAGEA

VKGTAEVGTT

PATATTPADV

VTLPTGTTNP

ADPNATIEIR

QTPADEVVAP

LASGKATAKQ

TTIEVRNPDG

DATVAAPIVT

TATANEALTA

T'IVEVRDADG

DLATPTIDSI

TPGETITI IS FYNSSEQ4LA

EPEIKIAAPT

TAGQATAQQS

TGGNNGNGSN

YLPSTGE-KES

ASAAVLDIEL

LVVPPELRGN

VDIDLTEVNR

OLNAAVQALE

SLLGTIVTL

EVTAPTITGV

GANETLTAVA

EIRNAGGTVI

TVTAPTITGV

GANETLTAVA

IEVRDAAGTV

TAPTVDNITG

GDTLTVIGKD

DADGNVIATG

PSVDKVTGNT

'IVNVVAKNDT

TI IGTTTqTDD TVTC7TTATGY

IAKDAAGKES

TVLGMATTGT

TNSSKGYEI

KDGAGNESQP

SGNITTGGTF

VSSVLGTSKA

LLATATDGAG

TGSNPNGGSG

SAVTTSLFCA

LSNVTSNNDS GTSTSNRWTA VAAAGSAAIN TNVTIDLSKV

QLELVNNIEN

AKGTS IPSNL

PTTVSTPQNL

TGNSTAGYEV

KNASGTEXTP

CTGTADCTCA

TGNSTAGYEV

KNASGTESTP

LGTXITTCTDG

NSGSGYEITG

NAGNESQPTE

TADCTCSFAV

TQGYQVTGTA

GLESQPTTAM

QCNPTVDLPA

QVTGTAEPNV

NPTAFKTPAD

DGKYTVTLEP

TGTAEPKTTII

ATAVIPADVV

SVHIAAGLAT

GYLIKGTAEP

HYSTATI'FMT

LGTI'GSGLGS

FVALLASMC I

LGAASFTAPE

VAAAINAALL

SNNLDARFVG

KGTADANATV

TTFQTPADEA

FTVTVPAGEA

KGTADANATV

TFQTPADPN

KY IVTLEDSCT

TADPNTTIEV

VLVPADATVT

NLPAGTANAN

ELG'ITIEVRA

TPADVTTPTI

GAANPGD'rLT TIE IRNEAGL

PDAPVATPTV

GKASANETIT

DVRDADGTI I LAAPTiTKvE

EKETLTALTT

NRIIQISNRL

PADPTNPCG

LC-NGLGTNGS

IXRXKhCN

ANQNQPVNFT

TFLTAVLNAA

TLAADGSYIS

PVKG'IVNVAV

TVVQTDLLDV

EIRNAGGTVI

TVTAPT ITO V

GANETLTAVA

EIRNAGGAVI

TPVATP IVET

ATANQTLSVV

RDPSGAVIGT

APTVTGVTGN

ETLTALAKDP

TOG'IVLGTAT

GDITGDS=T

VVGXDGDGNE

VIATGTTDGA

DI(ITGSTTNC

VVAKNATGKE

AATTAN.TCGQ

GNKANGYTVT

DTQGNVSPKT

LRSVIAVGAT

NGN~TGCNNGN

GYHPKLSTIS

VSGGALADAS

NDLTNVITQ I API SDGLGLV

SGALPLLAVG

NLLATADGVS

GTGTADGTGA

TGNSTAGYEV

KNASGTESTP

GTCTADGTGA

VTGSTTKGYE

AKNASGTESQ

GTSDANGDFT

SVAGYQVTGT

AGNTSTPTTF

TGPTGQYTVT

YEITGTADPN

SQPTEVTVPE

GA:FTITLPTG

YQVVGAAEVG

SQPATATTPV

YTVTLPACVV

GTADPNVTV/Q

'rFMTPAD ITO

DAEGNFAIQL

TGGNTGNNGA

YGTGNHGKTOG

155 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

EF068-3 (SEQ ID NO:259) CTC TGGCACAATC AGTGCATCAG CAGCGGTCTT GGATATCGAA

CTATTATCAA

GCCGCAA ACC

TCCGCTGTGT

AATGTAGCTG

GTTACTTT

ATTACCAGTG

CGTCAATTCG

GAAACGTTAG

GTTTAGCCC

GAG-GCAAAAG

CTTCCTGTCA

GGTGGTTCAG

TTACCAACTA

GGAACAGTCG

TCCAACATTT

GTAACAGGTA

GTGAAATCC

GCGTTTACAG

GCGAAAAACG

GCAACCGTAA

GTrAAAGGAA

ATAGGCACAG

GCAGGTGCCA

CCAACAACGT

GTGACAGGTA

GTTGAGATCC

GCAITTTACAG

GCGAAAA-ACG

ATGTTACGTC

AAAATCAACC

TTAGTGGACA

CAGCAGGCAG

TGACTCCCGT

GGGCGTT1AGG

AAT'AGTITAA

CAGCTGACGG

AAAATG'FrTC

GTACCAGTAT

AACCCACGGT

GCGTAAATGA

CCGTTTCAAC

FI'CAAACAGA

ATTTTGCTGC

ATTCAACAGC

GAAATGCAGG

TTACCGTTCC

CCAGCGGNAC

CCGCACCAAC

CTGCCGATGC

GTACCGCTGA

ATGAAACGTT

TCCAA.ACACC

A'ITCAACAGC

GAAATGCAGG

'ITACCATTCC

CCAGCGGTAC

AAATAATGAC

AGTTAATTTC

AAAACAAGCG

CGCAGCAATC

TFTGAATG-CA

GAATTTAACT

TAACATTGALA

CTCATACAT

AAACATCTI'A

CCCAAGTAAT

AAACGGCT

GTTAGTGGAT

ACCTCAAAAT

TCTTTTAGAC

AGGCACTACT

AGGTTACGA6A

AGGCACCGTA

CGCAGGTGA6A

AGAAAGNACG

AATCACAGGA

CAATGCCACG

TGGGACAGGA

AACCCCGTA

AGCGGATGAA

AGGTTACGAA

AGGTGCCGTG

CGCAGGTGAA

AGAAAGTACG

AGTGGCACTT

ACGG'TrCTG

GTGTTAGTGG

AATACCAATG

GCCAATGATT

GGTC'ITGATA

AACI'AGGTG

AGTGCACCGA

CAAGATTTGA

CTTGTCGCCG

G?ITCAGGTG

GCTTCTTTAC

TTATCCAATA

GTTAATTTAT

AGTGAAGTAA

GTTAAAGGAA

ATAGGCACAG

GCAGGCGCCA

CCAACAACGT

CGACACCTA

GTTGAAATCC

GCGTTTACAG

G^CGAAAAACG

GCAACCGTAA*

GTTAAAGGAA

ATAGGTACAG

GCACC.TGCGA

CCAACPLACGT

CAACGAGTA6A

GTGGCGCT

TTCCTCCTGA

TCACGA?1'GA

TAACCAATGT

TTGATTTGAC

CTGC=CATT

TTAGTUATGG

ATGCGGCAGT

CAGCTATAAA

C'LTTGCC'TI

TAGGCACAAC

ATTAGAT-GC

TAGCAACAGC

CCGCACCAAC

CTGCCGATGC

GTACCGCTGA

ATGAAACGTT

TCCA-AACNCC

ATTCAACGGC

GAA-TGCAGG

TTACCGTTCC

CCAGCGGCAC

CCGCACCAAC

CTGCCGATGC

GTACTGCTGA

ATGAAACGT

TCCAAACGCC

TCGTTGGACA

AGCAGATGCT

GTTAAGAGGA

TCTTCAAALA

GATTACTCAA

GGAAGTGAAT

TACAGCTCCG

TTAGGGTTA

TCAAGCTTTG

TGCAGCCTC

ATTAGCGGTT

CACGGTTACT

TCGTTTTGTA

AGACGGTGTA

AATCACAGGA

CAATGCCACG

TGGGACAGGA

AACCGCCGTA

AGCGGATGA6A

AGGTTACGAA

AGCCACCGTA

CGCAGGTGA6A AGAA6AGTACG

AATCACAGGA

CAATGCCACG

TGGGACAGGG

AACCCCGTA

o e 0 o 0 00 0 0 0 0 0 000000 0 0 EF068-4 (SEQ ID NO:260) TSGTIS ASA.AVLDIEL LSNVTSNNDS GTSTSNRIWTA ANQNQPVNFT VSGOALADAS

AVFSGQKQAV

TSGALGNLTG

LAQNVSNILQ

GSGVNELVDA

NIYFAAGTTS

FTV'IVPAGCEA

LVVPPELRGN

VDIDILTEVNR

DLNAAVQALE

SLLGTTTVTL

EVTAPTITGV

GANETLTAVA

VAAAGSAAIN

QLELV14NIEN AXGTS IPSNL

PTTVSTPQNL

TGNSTAGYEV

KNASGTEXTP

TNVTIDLSKV

LGAASFTAPE

VAAAINAALL

SNNLDARFVG

KGTADAMV

TTFQTP

TFLTAVLNAA

TLAADGSYI S

PVKGTNVAV

TVVQTDLLDV

EIRNAGG'IVI

NDLTINV ITQ I APISD3LGLV

SGALPLLAVG

NLLATADGVS

GTGTADGTG.A

EF069-1 (SEQ ID NO:261)

TAGGGGAAGC

ATGAAAAAGA

CGCAAGATGC

GTAAGTATAG

CTATIATCAA

GCCGCAAACC

TCCGCTGTGTT

AATGTAGCTG

GTTACTT=

TAATGATCTT

AATTGI'GA

TTAAT ITAGC

CTCTTACCTC

ATGTTACGTC

AAAATCAACC

'ITAGTGCACA

CACCAGGCAG

TGACTGcCGT

GGTATITATC

GGATTTTAAT

AATATCAAGT

TGGCACAATC

AAATAATGAC

AGTTAATTTC

AAAACAAGCG

CGCAGCAATC

TTTGAATGCA

GTrI'ATTTTA

CGGAAAAGTC

GGTTI'AITAT

AGTCGCATCAG

AGTGGCACTT

ACGGTTTCTG

GTCTTAGTCG

AATACCAATG

GCCAATGAT'

AAGAAAAGAG CGACGATCAG AGCATAAAA ATGGACAAA-A ITACGTCATT AGcAATCCCT CAGCGGTC IT GGATATCGAA CAACGAGTAA TCGTTGGACA GTGGCGCTTT AGCAGATGCT ?I'CCTCCTGA GTTAAGAGGA TCACGA7M'A TCT2I'CAAA-A TAACCAATGT GATTACTCAA 156 TABLE 1. Nucleotide and Am~ino Acid Seqeuences of E. fuecalis Genes.

a.

a a.

a

ATTACCAGTG

CGTCAATTGG

GAAACGTTAG

GT'TTTAGCCC

GAGGCAAAAG

CTTCCTGTCA

GGTCGTTCAG

TTACCAACTA

GGAACAGTCG

TCCAACATT

GTAACAGGTA

GTTGAAATCC

GCGTTI'ACAG

GCGAAAAACG

GCAACCGTAA

GTTAAAGGAA

ATAGGCACAG

GCAGGTGCCA

CCAACAACGT

GTGACAGGTA

GTTGAGATCC

GCATTTACAG

GCGAAAAACG

AATACCCCCG

GAGGTCAAAG

GTCCTTGGTA

ACACCAACAG

CAACCAGCAA

GGCAACTCTG

C'ITCGTGATC

ACTGTAACGC

GATAACGCGG

ACAGCACCAA

ACCGCTCATC

GGGACTGCCG

AATGAAACAT

TTCCAAACAC

ACAACACAAG

GCAACAGACG

ACCTTAGCTT

ACTGGACTTrG

ATTGGTGACA

AATACCACCA

GATCA.AGGAA

ACAGTTGTT'G

GAAGATGCAA

TATCAAGTAA

TTAGTTA=-

GGCACAGCAA

ACTAATCCGP

GTTGACAAAP

GGTACAACAC

ACTGATGC;

ACTGTCGTAC

GGGCGTTAGG

AATTACTTAA

CAGCTGACGG

AAAATGTTTC

GTACCAGTAT

AAGGCACGGT

GCGTAAATGA

CCGTTTCAAC

TTCAA.ACAGA

ATTT'TGCTGC

ATTCAACAC

GAAATGCAGG

TTACCGTTCC

CCAGCGGNAC

CCGCACCAAC

CTGCCGATGC

GTACCGCTGA

ATGAAACGTT

TCCAAACACC

ATTCAACAGC

GAAATGCAGG

ITACCATTCC

CCAGCGGTAC

TGGCGACGCC

GGACMCTGA

CTGCAACAAC

CAAATCAAAC

CGGCGACAAC

G 1TCGGGTTA

CATCTGGGGC

TACCAACGGC

GAAATGAAAG

CTGTAACAGG

CGAATCCTAC

ATGGGACTGG

TGACAGCGTT

CAGCAGATGA

GATATCAAGT

GAACAGTI I

CAGGAA.AAC

AGAGTCAACC

TTACTGGAGA

TI'GAAGTACG

ACTTACTGI

GAAAAGACGG

CCGTAGCAGC

CCGGCACGGC

CTACGGGAAC

CAGCTAACGP

CTGCTTTCAz

TCACTGGTAC

TTGAGGTGCC

LA.ATACACAGJ

;CGAAAAA=G

GAATTTAACT

TAACATTGAA

CTCATACATT

AAACATCTTA

CCCAAGTAAT

AAACGTGGCT

GTT!AGTGGAT

ACCTCAAAAT

TCTTTTAGAC

AGGCACTACT

AGGTTACGA6A

AGGCACCGTA

CGCAGGTGAA

AGAAAGNACG

AATCACAGGA

CAATGCCACG

TGGGACAGGA

AACCGCCGTA

AGCGGATGAA

AGGTTACGAA

AGGTGCCGTG

CGCAGGTGAA

AGAAAGTACG

AATTCTTGAG

AGTTGGCACC

TGGAACTGAC

GCTGCCTT

ACCAGCTGAT

GGTGTTGATA

AAC'?TAGGTG

AGTGCACCGA

CAAGATTTGA

CTTGTCGCCG

GTTTCAGGTG

GCTTCTTAC

TTATCCAATA

GTTAATTTAT

AGTGAAGTAA

GTTAAAGGAA

ATAGGCACAG

GCAGGCGCCA

CCAACAACGT

GTGACAGGTA

GTTGAAATCC

GCGTTTACAG

GCGAAA.AACG

GCAACCGTAA

GTrAA.AGGAA

ATAGGTACAG

GCAGGTGCGA

CCAACAACGT

ACTCTAACAG

ACCATTGAG

GGAAAATATA

GTAGCGAAAA

GTCACTGCAzC rTGA=rrAC G ZTGCITCATT TrAGTCGATGG TI k.TGCGGCAGT T1 CAGCTATAAA TAGGCACAAC C A.TTTAGATGC I3

T'AGCAACAGC

CCGCACCA.AC

CTGCCGATGC C

CTACCGCTGA

A.TGAAACGTI'

rCCAAACNCC

ATTCAACGGC

GA.ATGCAGG

TTACCGTTCC

CCAGCGGCAC

CCGCACCAAC

CTGCCGATGC

CTACTCCTGA

ATGAAACGTT

TCCAAACGCC

GTAGTACAAC

TTCGCGATGC

CAGTGACTTT

ACGCTAGTGG

CAACAGTTGA

ACCCTAACAC

CTGATGCGAA

CGTTAACAGT

TTCCTGCTGA

CTGG'-rATCA

ATGGGAACGT

CAGCTGGGAC

ATACAAGTAC

TCGACAAAGT

GCACCACCAT

CGACTGGCCA

ATGTAGTTGC

CTrGATGTTAC

TCACTGGGAC

TTGGTACAAC

CTAATCCTCG

CAACGGAAGT

CAGGAACAAC

AGATI'CACAA

?I'ACAATCAC

AAGATGCTGC

CACCAGTCGC

TAGTAGGAGC

TTGGCATGGC

CC-TCAGCTAA

CAGCTACAC

GAAGTGAAT

'ACAGCTCCG

~TTAGGGTTA

CA.AGCTTTG

.'ICAGCCTI'G

~TTAGCGGTT

ACGGTTACT

[CGTTGTA

GACGGCTA

ATCACAGGA

AATGCCACG

L'CGGACAGGA

ACCGCCGTA

kGCGGATGA.A k.GGTTACGAA

AGGCACCGTA

CGCAGGTGAA

AGAAAGTACG

A.ATCACAGGA

:AATGCCACG

TCGGACAGG

A.ACCGCCGTA

AGCGGATCCT

AAAAGGCTAT

AGCTGGCACG

AGATTCAGGA

CACGGAAAGT

TAACATCACA

AACAATCGAA

TGGTGATTT

GATTGGAAAG

TGCCACGGTT

GGTGACAGC

GATTGCAACA

GGCAAATGCG

ACCGACAACC

TACTGGGAAT

TGAAGTI'CGT

ATATACTGTG

TAAAAATGAT

CACACCAACA

GGcGGACCCT

CACAACCGAT

TGATACATTA

GACGGTCCCT

TGCCACTGGG

TGAAGCAGGT

TCTTCCGACG

TGGGAAAGAA

GACACCTACT

AGCAGAAGTT

AAcTACTGGA

CGAAACAATA

AACTACACCA

TGAAATTACA GGAACAGCAG AGTCATTGGT ACAGGTACCT AACGACCAAT CCTGGGGATA TCAACCGACT GAAGTCCTTG a .q~

AGTAACAGGT

CATCGAAATT

TTCCTTTGCT

AGCCAAAGAT

AGTAGTGGCA

GACAGGTACC

AGGCACCGCA

AACAGCTAAA

AACTACAGCT

TTCAACAACT

AATTCAG'ITG

CGTGATGCAG

GTGAACC'ITC

CCTGCTGGCA

CCGCCAAGTG

GCTGAACTTG

ACAACTGGAC

CAAACAGTGA

ATGACACCCG

GGTTATGAAA

GAACCCAGAT GGAACAATrA GGACCTTCCA GCGGGAGCCG

TGACGGCAAT

ACCAACTGTG

AGAGCCAAAT

GACTGSATG.GT

AGCCTI'AACT

AACACCTGCT

CACGACAAAC

TGACGCCGAT

GACTTTAGAG

AACAGGAAAA

GAAAGTCAAC

ACCACTOTTA

GTCACCATTG

GC'TGGCGCAT

GCCATTGCGA

GATCCAGATG

GGCTATCAAG

GGCACAGTCC

CCAGGGAAGG

GAAAGTCAGC

157 TABLE 1. Nucleotide and Amnino Acid Seqeuences: of E. faecali-v Genes.

GTCGACTTAG

ATCACTCGGAA

ATTGCTGCTA

GTGACACCAG

CCAGCTACAG

GAAGGAA.ACA

CAATTTTACA

ITCTCCCTTC

ACAGATACAC

GGAGAACCAG

GCCGGCTACC

CTATTAAGAA

TTAACAGCGG

GGACATTACA

GGCAATGGTA

GCAACTGGCG

GGTTTAGGCA

AGTGGCTACC

GGCTACTTAC

GCCTTTGTCG

CCACACCAAC

CGGCGGAGCC

CAACTGCTAA

GAGAAACGAT

CCGTTATTCC

AACCCAATGG

ATAGCAGTGA

ATATTGCAGC

AAGGAAATGT

AGA'FTAAAAT

TCATCAAAGG

GTGMATTGC

GACAAG CGAC

GTACGGCTAC

ACACTGGCGG

GGAATAATGG

CAACAGGTTC

ACCCTAAACT

CTAGCACAGG

CACTCCF1'GC

CATTGATTCT

AAAAACCACT

CGAAACCGCC

TACGATTATT

AGCGGATGTT

CTATACAGTC

ACAATTATTG

AGGGTTAGCA

GAGTCCTAAA

TGCGGCACCA

AACAGCTGAA

TCTAGGTGCC

TGCTCAACAA

AACCTTCATG

AAATAACGGC

GAATGGTTCA

TGGCTTAGGT

AAGTACCATC

TGAAAAAGAG

GAGCATGGGA

A'ITACCGGAA

ATTGCATGTCC

CAATAkTACGG

AGCAAAGATG

GTC''AGCGG

ACTGGAACTC

GCAAGTGGCA

ACAGAAA-AAG

ACCACATTTA

ACTG=TCTT

CCAAACCGAA

ACCdAtC;CTC AGTTTAC PT

ACGCCAGCCG

AATACAGGCG

AACACAGGTI'

TCACTAGGCA

AGTTATrGGCA

TCTCAGCCC

ATCATCAAAC

ATT'TACTAA

GTGACGCAGA

TGACTCTACC

GCGCAGGTAA

CC-AACTAT

CTGATCCAAA

ATACAACTAC

A-AACGTTAAC

TrGACGCCAGC

CAGTTTAGG

TCATTCAAALT

AAGGCAACTT

CGACAGCTAC

ACCCAACGAA

GCAATACACG

CAAATCCAAA

ATGGCCTCGG

CTGGAAA'ICA

TGACAACAAC

GCAAACGT-7AA

AGGTTACGAA

CGGAACCATC

AGCTCGCGTA

TGAAAGTCAA

TACGAAGGTT

TGTCACGGTT

TGGAGGTACC

CGCACTAACC

CCATATTACC

AACGTCTAAA

TAGTAACCGA

CGCTATCCAA

CGATGGCGCA

TCCTGGAGGA

AAACAATGCC

TGGAGTTCT

TACAAATGGT

CGGGAAAACA

TTTGTTTGGC

AAACTAG

S

S

*5 EF069-2 (SEQ ID NO:262) M KKKIVEDFNR KSQHKKWTKR KME..LAISSG LLFTSLAIPV *5*q 5* 5

S*

S IAVTSGTIS

AVFSGQKQAV

TSGALGNLTG

LAQNVSNI LQ

GSGVNELVDA

NIYPAAGTTS

FIVTVPAGEA

KGTADANATV

TTFQTPADEA

FTVTI PAGEA

VKGTAEVGTT

PATATTPADV

VTLPTGETNP

ADPNATIEIR

QTPADEVVAP

LASGKATAKQ

TT-IEVRNPDC

DATVAAPIVT

TATANEALTA

TTIVEVRDADG

DLATPTIDS I

TPGETITIIS

FYNSSEQLLA

EPEIKIAAPT

TAGQATAQQS

TGCNNGNGSN

YLPSTGEKES

ASAAVLEJIEL

LVVPPELRGN

VDI DLTEVNR

DLNAAVQALE

SLLGTTTVTL

EVTAPTITGV

GANETLTAVA

EIRNAGGTVI

TVTAPTITGV

GANETLTAVA

IEVRDAAGTV

TAPIVDNITG

GDTLTVIGKD

DADGNVIATG

PSVDKVTGNT

TVNVVAKNDT

TI IGTTT'D

TVTGTTATGY

IAKDAAGKE-S

TVLGMATTGT

TGNSSKGYEI

KDGAGNESQP

SGNTTTGGTF

VSSVLGTSKA

LLATATDGAG

TGSNPNGGSG

SAVTTSLFGA

LSNVTSNNDS

VAAAGSAAIN

QLELVNNIEN

AKGTSIPSNL

PTTVSTPQNL

TGNSTAGYEV

KNASGTEXTP

GTGTADGTGA

TGNSTAGYEV

KNASGTESTP

LGTATTGTDG

NSGSGYFITG

NAGNESQPTE

TADGTGSFAV

TQGYQVTGTA

GLESQPTTAM

QGNFTVDLPA

QVTG7TAEPNV

NPTAFKTPAD

DGKYTVTLEP

TGTAEPKTTI

ATAVI PADVV SVH IAAGLAT GYL IKGTAEP

HYSTATTFMT

LGTTGSGLGS

F'JA-LASMG I

SNNLDARFVG

KGTADANATV

TTFQTPADEA

FTVTVPAGEA

KGTADANATV

TTFQTPADPN

KY'IVTLDSCT

TADPNTTIE:V

VLVPADATVT

NLPAGTANAN

ELGITIEVRA

TPADVTTPTI

GAANPGDTLT

TIE IHNE-AGL

PDAPVATPTV

GKASANETIT

DVRDADGTI I

LAAPTITKVE

EKETLTALTT

MRIIQISNRL

PADPTNPGGG

LGNGL.TNGS

IKRKRKN

TVVQTDLLDV

EIRNAGGEVI

TVTAPTITGV

CANETLTAVA

EIRNAGGAVI

TPVATP IVET

ATAN'QTLSVV

RDPSGAVIGT

APTVTGVTGN

ETLTALAKIDP

TDGTJLGTAT

GDITGDSTTG

VVGKDGDC;NE

VIATGTTDGA

DKITGS'ITNG

VVAKNATCKE

AATTANETGQ

GNKANGYT

DTQGNVSPKT

LRSVIAVGAT

NGNTGGNNGN

GYHPKLSTIS

GTSTSNRWTA ANQNQPVNFT TNVTI!JLSKV TFLTAVLNA-A LGAASFTAPE. TLAADC-SYIS VAAAINAALL' PVX<GTVNVAV

VSGGALADAS

NDLTNVITQ I AP ISDGLGLV

SCALPLLAVG

NLLATADGVS

GTGTADGTGA

TGNSTAGYEV

KNASGTESTP

GTGTADGTGA

VTGSTTKGYE

AKNASGTESQ

GTSDANGDFT

SVAGYQVTGT

AGNTSTPTrF TGPTGQY'rVT

YEITGTADPN

sQPTEvTvPE

GAFTITLPTG

YQVVGAAEVG

SQPATATTPV

Y IVTLPAGVV

GTADPNVTVQ

TFMTPADITG

DAEGNFAIQL

TGGNTGNNGA

YGTGNHGKTG

EF069-3 (SEQ ID NO:263) 158 TABLE 1. Nucleotide and Amino Acid Seqeuenccs of E. faecalis Genes.

AGGTGAA GCAGGTGCGA ATGAAACGTT AACCCCCGTA

GCGAAAAACG

AATACGCCCG

GGTCAAAG

GTCCTTGGTA

ACAGCAACAG

CAACCAGCAA

GGCAACTCTG

GITCGTGATC

ACTGTAACC

GATAACGCGG

ACAGCACCAA

ACCCCTGATC

GGGACTGCCG

AATGAAACAT

TTCCAAACAC

ACAACACAAG

GCAACAGACG

ACGTTAGCTT

ACTGGACTTG

ATTGGTGACA

AATACCACCA

GATCAAGGAA

ACAGTTGTT6

GAAGATGCAA

CCAGCGGTAC

TGGCGACGCC

GCACTCCTGA

CTGCAACAAC

CAAATCAAAC

CCGCGACAAC

GTTCGGGTTA

CATCTGGGGC

TACCAACGGG

GAAATGAAAG

CTGTAACAGG.

CGAATGCTAC

ATGGGACTC

TGACAGCGTT

CAGCAGATGA

GATATCAAGT

GAACAG'ITT

CAGGAA.AAGC

AGAGTCAACC

TTACTGGAGA,

TTGAAGTACG

ACTTTACTGT

GAAAAGACG

CCGTAGCAGC

AGAAAGTACG

AA'TTIGAG

AOTTCGCACC

TCGAACTGAC

GCTGAGCGTT

ACCACCTGAT

TCAAATITACA

AGTCATTGGT

AACGACCAAT

TCAACCGACT

AGTAACAGGT

CATCGAAAT

TTCCTTTGCT

AGCCAAAGAT

AGTAGTGGCA

GACAGGTACC

AGGCACCGCA

AACAGCTAAA

AACTACAGCT

TTCAACAACT

GAACCCAGAT

GGACCTTCCA

TGACGGCAAT

ACCA.ACTGTG

CCAACAACGT

ACTGTAACAG

ACCATTGAGG

GGAAAATATA

GTAGCGAAAA

GTCACTGCAC

TCCAAAC

GTAGTAC

TTCGCGI

CAGTGAC

ACGCTAC

CAACAG~

GGAACAGCAG ACCCTAJ ACAGGTACCT CTGATG( CCTGGGGATA CGTTAA( CAAGTCCTTG TTCCTG( AATTCAGTTI CTGGITI CGTGATGCAG ATGGGA CTGAACCTTC- CACCT( CCTGCTGCCA ATACAA( CCGCCAAGTC TCGACA GCTGAACTTIG GCACCA( ACAACTGGAC CGACTC CAAACAGTGA ATGTAG' ATGACACCCG CTGATG GGTTATGAAA TCACTG( GGAACAATTA TTGGTA( GCGGGAGCCG CTAATC GAAAGTCAAC CAACGCG ACGACTGTTA CAGGAA GCCc

:AAC

~TGC

:TTT

,TGG

[TGA

.CAC

CAGT

CTGA

.TCA

kCGT

'AC

GTAC

k.AGT

CCAT

XCCA

F1'GC E1AC

GAC

CAAC

CTGG

AAGT

AGCGGATCCT

AAAAGGCTAT

AGCTGGCACG

AGATTCAGGA

CACGGAA.AGT

TAACATCACA

AACAATCGAA

TGGTGATT

GATTGGAAAC

TGCCACGG TT

GGTGACAGGC

GATTGCAACA

GGCAAATGCG

ACCGACA-ACC

TACTGGGAAT

TGAAGTTCGT

ATATACTGTG

TAAAAATGAT

CACACCAACA

GGCGGACCCT

GACAACGGAT

TGATACATTA

GACGGTCCCT

S S

S

*555

S

SS

EF069-4 (SEQ ID NO:264) AGEA GANETLTAVA KNASGTEXTP ?1'FQTPADEA TVTAPTITGV TGNSTAGYEV KGTADANATV EIRNAGGTVI TTFQTPADEA TVTAPTITIGV FTVTIPAGE-A GANETLTAVA VKGTAEVGTT I EVRDAAGTV PATATTPADV TAPTVDNITG VTLPTGTTNP GDTLIVIGKD ADPNATIEIR DADGNVIATG QTPADEWVAP PSVDKVTGNT LASGKATAKQ IVNVVAKNDT TTIEVRNPDG TI IGTT'ITDD DATVAAPTVT TVTGT

GTGTADGTGA

TGNSTAGYEV

KNASGTESTP

LGTATTGTEDG

NSGSGYEITG

NAGNESQPTE

TAD)G LSFAV

TQGYQVTGTA

GLESQPTTAM

QGNFT'JDLPA

FTVTVPAGEA

KGTADANATV

TTFQTPADPN

KYTVTLOSGT

TADPNTTIEV

VLVPADATVT

NLPAGTANAN

ELG'ITIEVRA

TPADVTTPTI

CAANPGDTLT

GA.NETLTAVA

EIRNAGGAVI

TPVATPIVET

ATANQTLSVV

RDPSGAVIGT

APTV'MVTGN

ETLTALAKDP

TDGTVLGTAT

GDITGOSTTG

VVCKDGDGNE

KNASGTESTP

GTGTADGTGA

VTGSTIvTKGYE

AXNASCTESQ

GTSDANGDFT

SVAGYQVTGT

AGNTSTPTTF

TGPTGQYTVT

YEITGTADPN

SQPTEVTVPE

EF070-1 (SEQ ID NO:265)

TAGCGGAAGC

ATGAAAAAGA

CGCAAGATGC

CTAAGTATAG

CTATTATCAA

GCCGCAAACC

TCCGCTGTGT

AATGTAGCTG

GTTACTTr

ATTACCAGTG

TAATGATCTT

AAATITCTTGA

TI'AATTAGC

CTGTTACCTC

ATG-TTACGTC

AAAATCAACC

LTAGTG.GACA

CAGCAGGCAG

TGACTGCCGT

GGGCGTTAGG

GGTA'TrATC

GGAT='IAAT

AATATCAAGT

TGGCACAATC

AAATAATCAC

AGTTAATITTC

AAAACAAGCG

CGCAGCAATC

TFI'AATGCA

GAA IITAACT

GTTATTTTA

CGGAAAAGTC

GGTITATTAT

AGTr.CATCAG

AGTGOCACTT

ACGGI'TCTG

GTGTTAGTGG

AATACcAATG GCCAATGATr

GGTG'ITGATA

AAGAAAAGAG

AGCATAAAAA

TTACGTCAI'

CAGCGGTCT

CAACGAGTAA

GTGGCGCTTT

TTCCTCCTGA

TCACGATTGA

TAACCAATrCT

TTGATTTGAC

GGACGATCAG

ATGGACAAAA

AGCAATCCCT

GGATATCGAA

TCGTTGGACA

AGCAGATGCT

GTTAAGAGGA

TCTTTCAAAA

GATTACTCAA

GGAAGTGAAT

159 TABLE I. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

CGTCAATTGG

GAAACGTTAG

GTTTTAGCCC

GAGGCAAAAG

CT-TCCTGTCA

GGTGGTTCAG

TTACCAACTA

GGA.ACAGTCG

TCCAACATTT

GTAACAGGTA

GTTGAAATCC

GCGTTTACAG

GCGAAAAACG

GCAACCGTAA

GTTAAAGGAA

ATAGGCACAG

CCAGGTGCCA

CCAACAACGT

GTGACAGGTA

GTTGAGATCC

GCATTTACAG

GCGAAAAACG

AATACGCCCC

GAGGTCAAAG

GTCCTTGGTA

ACAGCAACAG

CAACCAGCAA

GGCAACTCTG

GTTCGTGATC

ACTCTAACC

GATAACGCGG

ACAGCACCAA

ACCGCTGATC

GGGACTCCC

AATGAAACAT

TrrCCAAACAC

ACAACACAAG

GCAACAGACG

ACGTTAGCTT

ACTGGACTrG

ATTGGTGACA

AATACCACCA

GATCAAGGAA

ACAGTTGTTG

GAAGATGCAA

TATCAAGTAA

TTAGTTATTG

GGCACAGCAA

AGTAATCCGA

GTTGACAAAA

GGTACAACAG

ACTGATCGCA

ACTGTCGTAG

GTCGAC ETAG AAI'AGTrTAA

CAGCTGACGG

AAAATGTTTC

GTACCACTAT

AAGGCACGGT

GCGTAAATGA

CCCTTICAAC

TTCAAACAGA

AT=~GCTGC

ATTCAACAGC

GAAATGCAGG

TTACCGTTCC

CCAGCGGNAC

CCCCACCAAC

CTGCCGATGC

GTACCGCTGA

ATGAAACGTT

TCCAAACACC

ATTCAACAGC

GAAATGCAGG

TTACCATTCC

CCAGCGGTAC

TGGCGACCCC

GGACTGCT--A

CTGCAACAAC

CAAATCAAAC

CGGCGACAAC

GTTCGGGTTA

CATCTGGGGC

TACCAACGCG

GAAATGAAAG

CTGTAACAGG

CGAATGCTAC

ATGGGACTIGG

TGACAGCGT

CAGCAGATGA

GATATCAAGT

GAACAGT1

CAGGAAAAGC

AGAGTCAACC

TTACTGGAGA

TAAGTACG

ACTTTACTGT

GAAAAGACGG

CCGTAGCAGC

CCGGCACC

CTACGGGAAC

CAGCTAACGA

CTCCTTTCAA

TCACTGGTAG

TTGAGGTGCG

AATACACAG'I

CGAAAAATGc

CCACACCAAC

TAACATTGAA

CTCATACATTr

AAACATCTTA

CCCAAGTAAT

AAACGTGGCT

GTTAGTGGAT

ACCTCAAAAT

TCITTTTAGAC

AGGCACTACT

AGGTTACGAA

AGGCACCGTA

CGCAGGTGA-A

AGAAAGRACG

AATCACAGGA

CAATGCCACG

TGGGACAGGA

AACCCCTA

AGCGGATGAA

AGGTTACGAA

AGGTGCCGTC

CGCAGGTGA-A

AGAAAGTACG

AATTC1'TTAC

AGTTGGCACC

TGGAACTGAC

GCTGAGCGTT

ACCACCTGAT

TGAAATTACA

AGTCATTGGT

AACGACCAAT

TCAACCGACT

AGTAACAGGT

CATCGAAATT

TTCCTTI'CCT

AGCCAAAGAT

AGTAGTCGCA

GACAGGTACC

AGGCACCGCA

AACAGCTAAA

AACTACAGCT

TI'CAACAACT

GAACCCAGAT

GGACCTTCCA

TGACGGCAAT

ACCAACTGTG

AGAGCCAAAT

GACTGATGGT

*AGCCTTAACT

*AACACCTGCT

CACGACAAAC

TGACGCCGAT

GACTFI'AGAG

AACAGGAAAA

CATTGATTCT

AkACTTlACCTGC

AGTGCACCGA

CAAGATTTGA

CTGTCGCCG

GTTTCAGGTG

GCTTCTT'TAC

TTATCCAATA

CTTAATTTAT

AGTGAAGTAA

GTTAAAGGAA

ATAGCCACAG

G&AGGCGCCA

CCAACAACGT

GTCACAGGTA

GTTrGAAATCC

GCGTTTACAG

GCGAAAA.ACC

r-CAACCGTAA

GTTAAAGGAA

ATAGGTACAG

GCAGGTGCGA

CCAACAACGT

ACTGTAACAG

ACCATTGAGG

GGAAAATATA

GTAGCGAAAA

GTCACTrGCAC

GGAACAGCAG

ACAGGTACCT

CCTGGGGATA

GAAGTCCTTG

AATTCAGTTG

CGT'GATGCAG

GTGAACCTTC

CCTGCTGGCA

CCGCCAACTC

GCTGAAC7-rX

ACAACTGGAC

CAAACAGTG.A

ATGACACCCG

GGTATGAAA

GGAACAATTA

GCGGGACCC

GAAAGTCAAC

ACGACTGTrA

GTCACCA=G

GCTGGCG-CAI

GCCATTGCGA

GATCCACATC

GGCTATCAAC

GGCACAGTCC

CCAGGGAAGC.

GAAAGTcAC ATTACCGGA-2 CGCT1CAT

TTAGT'GATGG

ATGCGC-CAGT

CACCTATAAA

CI'TGCCTT

TAGGCACAAC

ATTTAC-ATGC

TAGCA.ACAGC

CCGCACCAkAC

CTGCCGATGC

GTACCCCTGA

ATGAAACGTT

TCCAAACNCC

ATTCAACGGC

GAAATGCACG

TTACCGTTCC

CCAGCGGCAC

CCGCACCAAC

CMCCGATGC

GTACTGCTGA

ATGAAACGTT

TCCAAACGCC

GTAGTACAAC

TTCGCGATGC

CAGTGACTTT

ACGCTAGTGG

CAACAC ?TGA

ACCCTAACAC

CTGATGCGAA

CGTTAACAGT

TICCTGCTrGA

CTGGTTATCA

ATGGGAACGT

CAGCTGGGAC

ATACAAGTAC

TCGACAAAGT

GCACCACCAT

CGACTGGCCA

ATGTAG7MTC

CTGATG'ITAC

TCACTGGGAC

TTGGTACAAC

CTAATCCTGG

CAACGGAAGT

CAGGAACAAC

AGATTCACAA

T1'ACAATCAC

AAGAT-GCTGC

CACCAGTCGC

TAGTAGGAC

*TTGGCATGGC

CCTCAGCTAA

*CAGCTACAGC

ATTCTAGTAA

TACAGCTCCG

TTAGGGTTA

TCA.AGCTTTG

TGCAGCC'ITG

ATTIAGCGGTT

CACGGI'ACT

TCGTTTTGTA

AGACGGTGTA

AATCACAGGA

CAATGCCACG

TGGGACAGGA

AACCGCCGTA

AGCGGATGAA

AGGTTACGAA

AGGCACCGTA

CGCAGGTGAA

AGAAAGTACG

AATCACAGGA

CAATGCCACG

TGGGACAGGG

AACCCCGTA

AGCGGATCCT

AAAAGGCTAT

AGCTGGCACG

AGATTCAGGA

CACGGAAAGT

TAACATCACA

AACAATCGAA

TGGTGATTTT

GAI'GGAAAG

TGCCACGG T

GGTGACAGGC

GATTGCAACA

GGCAAATGCG

ACCGACAACC

TACTCGGAAT

TGAAGTTCGT

ATATACTGTG

TAAAAATGAT

CACACCAACA

GGCGGACCCT

GACAACGGAT

TGATAcA ITA

GACGGTCCCT

TGCCACTGGG

TGAAGCAGGT

TCTTCCGACG

TGGGAAAGAA

GACACCTACT

AGCAGAAGTT

AACTACTGGA

CGAAACAATA

AACTACACCA

AGGTTACGAA

160 TABLE 1. Nucleotide and Amino Acid Seqeuences of £.faecalis Genes.

ATCACTGGA-A

ATTGCTGCTA

GTGACACCAG

CCAGCTACAG

GAAGGAAACA

CAArr'ITACA

TTCTCCGT-TC

ACAGATACAC

GGAGAACCAG

GCCGGCTACC

CTATTAAGAA

TTAACAGCGG

GGACATTACA

GGCAATGGTA

GCAACTGGCG

GGTTTAGCCA

AGTGGCTACC

GGCTACTTAC

GCCTTTGTCG

CGGCGGAGCC

CAACTGCTAA

GAGAAACGAT

CCGTTATTCC

AAGCCAATGG

ATAGCAGTGA

ATAT1'GCAGC

AAGGAAATGT

AGATI'AAAAT

TCATCAAAGG

GTGTGATTGC

GACAAGCGAC

GTACGGCTAC

ACACTGGCGG

GGAATAATGG

CAACAGCTTC

ACCCTAAACT

CTAGCACAGG

CACTCCTTGC

AAAAACCACT ATTGATGTCC

CGAAACCGGC

TACGATI'ATT

AGCGGATGTT

CTATACAGTC

ACAATTATTG

AGGGTTAGCA

GAGTCCTA-AA

TGCGGCACCA

AACAGCTGA-A

TGTAGCTGCC

TGCTCAACAA

AACCTTCATG

AAATAACGGC

GAATGGTTCA

TGGCTTAGGT

A.AGTACCATC

TGAAAAAGAG

GAGCATGGGA

CAATATACGG

AGCAAAGATG

GTTrTTAGCGG

ACTGGAACTG

GCAAGTGGCA

ACACAAAAAG

ACCACATTTA

ACTGTTTCT.P

CCAAACCGAA

ACCGATGCTG

AGT TrACTTG

ACGCCAGCCG

AATACAGGCC

AACACAGG LT

TCACTAGGCA

AGTTATCCCA

TCTTCAGCCG

ATCATCAAAC

GTGACGCAGA

TGACTCTACC

GCGCAGGTAA

CGCCAACTAT

CTGATCCAAA

ATACAACTAC

AAACGTTAAC

TGACGCCAGC

CAGTTTrTAGG

TCATTCAAAT

AAGGCAACTrr

CGACAGCTAC

ACCCAACGAA

GCAATACAGG

CAAATCCAAA

ATGGCCTCGG

CTGGAAATCA

TGACAACAAC

GCAAACGTAA

CGGAACCATC

AGCTGGCGTA

TGAAAGTCAA

TACGAAGG Pr

TGTCACGCTT

TGGAGGTACC

CGCACTAACC

CGATATTACC

AACGTCTAAA

TAGTAACCGA

CGCTATCCA

CGATGGCGCA

TCCTGGAGGA

AAACAATGGC

TGGAGGTTCT

TACAAATGGT

CGGGAAAACA

TTTGTTTGGC

AAACTAG

EF070-2 (SEQ ID NO:266) M KKKIVEDFNR KSQHKKWTKR XMLNLAISSG'LLFTSLAIPV a a

SIAVTSGTIS

AVFSGQKQAV

TSGALGNLTCG

LAQN'VSNI LQ

GSGVNELVDA

NIYFAAGTTS

FTVTVPAGEA

KGTADANATV

TTFQTPADEA

FTVTI PAGEA VKGTAEVGTr

PATATTPADV

VTLPTGTTNP

ADPNATIEIR

QTPADEVVAP

LASGKATAKQ

TTIEVRNPDG

DATIVAAPTVT

TATANE-ALTA'

TTVEVRDADG

DLATPTIDSI

TPGETITI IS

FYNSSEQLLA

EPEIKIAAPT

TAGQATAQQS

TGGNNGNGSN

YLPSTGEKES

ASAAVLDIEL

LVVPPELRGN

VD IDLTEVNR

DLNAAVQALE

SLLGTTTVTL

EVTAPTITGV

GANETLTAVA

EIRNAGGTVI

TVTAPTITCV

GANETLTAVA

IEVRDAAGTV

TAPTVDNITG

CDTLTV IGKD

DADGNVIATG

PSVDKVTGNT

TVNVVAKNDT

TI IGTTTTDD

TVTGTI'ATGY

IAKDAAGKES

TVLGMATrGT

TGNSSKGYEI

KDGACNESQP

SGN'rTTGGT'F

VSSVLGTSKA

LLATATDGAG

TGSNPNGGSG

SAVTTSLFGA

LSNVTSNNDS

VAAAGSAAI N

QLELVNNIEN

AKGTSIPSNL

PTTVSTPQNL

TGNSTAGYEV

KNASGTEXTP

GTGTADGTGA

TGNSTAGYEV

KNASGTESTP

LGTATTGTDG

NSGSGYE ITG

NAGNESQPTE

TADGTGSFAV

TQGYQVTGTA

CLESQPTTAM

QGNFTVDLPA

QVTGTAEPNV

NPTAFKTPAD

DGKYI'JTLEP

TG TAEPKTTI ATAVI PADVV

SVHIAAGLAT

GYLIKGTAEP

HYSTATI'FMT

LGTTGSGLCS

FVALLASMGI

GTSTSNRWTA

TNVTIDLSKV

LGAASFTAPE

VAAAINAALL

SNNLDARFVG

KGTADANATV

TTFQTPADE-A

PIVTVPAGEA

KGTADANATV

TTFQTPADPN

KYTVTLDSGT

TADPNTTIEV

VLVPADATVT

NLPAGTANAN

ELGTTIEVRA

TPADVTTPTI

GAANPGDTLT

TIE IHNEAGL

PDAPVATPTV

GKASANETIT

DVRDAIJCTI I

LAAPTITKVE

EKETLTALTT

NRIIQISNRL

PADPTNPGGG

LGNGLGTNGS

IKRKRKN

ANQNQPVNFT

TFLTAVLNAA

TLAADGSYIS

PVKGTVNVAV

'TVVQTDLLOV

El RNACGIVI

TVTAPTITGV

GANETLTAVA

EIRNAGGAVI

TPVATP IVET

ATANQTLSVV

RDPSGAVIGT

APTVTCVTGN

ETLTALAKDP

TDGTVLGTAT

GDITGDSTTG

VVGKDGDGNE

VIATGTDGA

DKITGSTTNG

VVAKNATGKE

AATTANETGQ

GNKANGYIVT

DTQGNVSPKT

LRSVIAVGAT

NGNTGGNNGN

GYHPKLSTIS

VSGGALADAS

NDLTNVITQI

APISDGLGLV

SGALPLLAVG

NLLATADGVS

GTGTADGTGA

TGNSTAGYEV

KNASGTESTP

GTGTADGTGA

VTGSTTKCYE

AKNASGTESQ

GTSDANGDFT

SVAGYQVTGT

ACNTSTPITF

TGPTGQYTVT

YEITGTADPN

SQPTEVTVPE

GAFTITLPTG

YQVVGAAEVG

SQPATATTPV

YTVTLPAGCW

GTADPNVTVQ

TFMTPADITG

DAEGNFAIQL

TGGNTGNNGA

YGTGN14GKTG EF070-3 (SEQ ID NO:267) CGG TCACGCCAAT GAAAGTCAAC CAACGGAAGT GACGGTCCCT 161 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

GAAGATGCAA

TATCAAGTAA

TTAGT'ATTC

GGCACAGCAA

AGTAATCCGA

GTTGACAAAA

GGTACAACAG

ACTGATGGCA

ACTGTCGTAG

GTCGAC'ITAG

ATCACTGGAA

ATTGCTGCTA

GTGACACCAG

CCAGCTACAG

GAAGGAAACA

CAAT=rACA

TTCTCCGTTC

ACAGATACAC

GGAGAACCAG

GCCGGCTACC

CTATTAAGAA

TTAACAGCGG

GGACATTACA

GGCAATGGTA

GCAACTGGCG

GGT'ITAGGCA

AGTGGCTACC

GGCTACT

CCGTAGCAGC

CCGGCACGGC

CTACGGGAAC

CAGCTAACGA

CTGCTTTCAA

TCACTGGTAG

TTGAGGTGCG

AATACACAGT

CGAAAAATGC

CCACACCAAC

CGGCGGAGCC

CAACTGCTAA

GAGAAACCAT

CCGTTATTCC

AAGCCAATGG

ATAGCAGTGA

ATATTGCAC

AAGGAAATCT

AGATTAAAAT

TCATCAAACG

GTGTGATTGOC

GACAAGCGAC

GTACGGCTAC

ACACTGGCGG

GGAATAATGG

CAACAGGT'C

ACCCTAAACT

ACCAACTGTG

AGAGCCAAAT

GACTGATGGT

AGCCTTAACT

AACACCTGCT

CACGACAALAC

TGACGCCCAT

GACTTTAGAG

AACAGGAAAA

CATTGATCT

AAAAACCACT

CGAAACCGGC

TACCATTAT

AGCGGATGTT

CTATACAGTC

ACAATTATTG

AGGGTTAGCA

GAGTCCTAA-A

TCCCCCACCA

AACAGCTGAA

TGTAGGTGCC

TGCTCAACAA

AACCTTCAC

AAATAACGGC

GAATGGTI'CA

TGGCTTAGGT

AAGTACCATC

ACGACTCTTA CAGGA.ACAAC GTCACCATTG AGATTCACAA GCTGGCGCAT TTACAATCAC GCCATTGCGA AAGATrGcTrc

GATCCAGATG

GGCTATCAAG

GGCACAGTCC

CCAGGGAAGG

GAAAGTCAGC

ATTACCGGAA

ATTGATGTCC

CAATATACGG

AGCAAAGATG

GTTT TAGCGG

ACTGGAACTG

GCAAGTGGCA

ACAGAAAAAG

ACCACATTTA

ACTGTTTCTT

CCAAACCr.AA

ACCGATGCTG

AGMTACTI'

ACGCCAGCCG

ALATACAGGCG

AACACAGGTr

TCACTAGGCA

AGTTATGGCA

CACCAGTCGC

TAGTAGGAGC

'ITGGCATCGC

CCTCAGCTAA

CAGCTACAGC

ATI'CTAGTAA

GTGACGCAGA

TGACTCTACC

GCGCAGGTAA

CGCCAACTAT

CTGATCCAAA

ATACAACTAC

AAACGTTAAC

TGACGCCAGC

CAGTTTTAGG

TCATTCAAAT

AAGGCAACTT

CGACAGCTAC

ACCCAACGAA

GCAATACAGG

CAAATCCAAA

ATGGCCTCGG

CTGGA.AATCA

TGCCACTGGG

TGAAGCAGGT

TCTTCCGACG

TGGGAAAGAA

GACACCTACT

AGCAGAAGTT

AACTACTGGA

CGAAACAATA

AACTACACCA

AGGTTACGAA

CGGAACCATC

AGCTGGCGTA

TGAAAGTCAA

TACCAAGGTT

TGTCACGGTT

TGGAGGTACC

CGCACTAACC

CGATATTACG

AACGTCTAAA

TAGTAACCGA

CGCTATCCAA

CGATGGCGCA

TCCTGGAGGA

AAACAATGGC

TGGAGGTTCT

TACAAATGGT

CGGGAAAACA

S.

S

55 S

S*

*S

EF70-4 (SEQ ID NO:268) DGDGNE SQPTEVTVPE DATVAAP'IVT TVTG'FrATGY TATANEALTA IAKDAACKES TTVEVRDADG TVLGMATT DLATPTIDSI TGNSSKGYEI TPGETITIIS KDGAGNESQP FYNSSEQLLA SGN'TITGGTF EPEIKIAAPT VSSVLGTSKA TAGQATAQQS LLATATDGAG TG.GNNGNGSN TGSNPNGGSG

QVTGTAEPNV

NPTAFKTPAD

DGKYTVTLEP

TGTAEPKTTI

ATAVI PADVV

SVHIAAGLAT

GYLIKGTAEP

HYSTATTFMT

LGTTGSGLGS

TIEIHNEAGL

PDAPVATPTV

GKASANETIT

DVRDADGTII

LAAPTITKVE

EKETLTALTr

NRIIQISNRL

PADPTNPGGG

LGNGLOTNCS

VIATGTTOGA

DKITGSI'NG

VVAKNATGKE

AATTANETGQ

GNKANGYTVT

DTQGNVSPKT

LRSVIAVGAT

NGNTGGNNGN

CYIIPKLSTIS

GAFTITLPT'G

YQVVGAAEVG

SQPATATTPV

Y7VTLPAGVV

GT.ADPNVTVQ

TFMTPAD ITG DArEGNFAIQL

TGGNTGNNGA

YGTGNHGKTG-

EF071-1 (SEQ ID NO:269)

TAAGTAGAAC

GTCCCGCCAT

ATGGC'ITTTT

GTGATTGGTT

CCAATCAACT

TCTGGAAkACG

CGAAATTGGT

TGGTCGGGAC

TTATCTGCAG

TAAGAAAGGA

TAAG=TAAC

TTAC'ITATTT

AGCGGAACCT

CAAATGCTTA

AAACGTAGAA

GTrrAAGCCG

GCATGCTATG

GATTCCGATG

TCCCGGCTCT

AGGACCACAC

TCTCTCATAT

CTT7CGCTGA

TGGAAGGGAA

TTTAAAAAAT

ACGGCTTNCG

GCAAGCAATG

AGmAcACA

TTTGTCGGAT

TI'GCCGAAGA

GTTATI I'A

TAATGATTCA

CTACACCAT

AATI'AAMCT

ATGAAGTGGC

cTGGTGGACG

ATACTTCT

CTTTCCTTTC

ACTTGCTrI'A

CGAAGCGGAT

T'ITACATGAA

CTATATGAAA

AGTGAAACAA

162 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

TTAGCTCCTIG

GCGCAAATCG

TATGTTAATT

GGAACAGGTT

ACAGATCCTT

ACAACGGGCG

CTGGCCAAAT

AATTGGCTCG

TGGCCCGTGA

ATGGCATAGT

ATGGTTAT-T

T7CNGNAAC TCAATATGGC GCAGGTTCTT AACGAATAAT GCGGCGACAT TTTGGCTCAG AACATTGGTG

CACTCATGAT

AGCGCGTIGGG

AGGTGAGACT

TGGATTACAA

GGATT~AGTAA

GGTCATTATT

TACCTAATCA

TTAAAAAAGA

CTCATTTTTC

A-AAAT"=CTG

AGCZ-CATTGG

CAGCCAGGTA

AAAAGCCTAT

TTATGCTGCC

TCTGGACGAT

GGGAGATCAT

CACNAGATTT

EF071-2 (SEQ ID NO:270) MF KKLNIQLALV IGLSLTIPMT AXAYTIEADP INFTYFPGSA SNELIVLHES GNERNLGPHS LDNEVAYMKflR NWSNAYVSYF VCSCCRVKQL APAGQIQYGA GSLANQKAYA QIELARTNNA ATFK KDYAAY VNLARDLAQN IGAOFSLDDG TG-YGIVTHDW ITXNWWGDHT OPYGYIJARCG LNKRIGTRFT TGVSXTGETC HYSAR EF071-3 (SEQ ID NO:271) G TTTAAAAAAT TAATGATTCA ACTTGCM-=A

V

GTGATTGGTT

CCAATCAACT

TCTGCAAACC

CGAAATTGGT

TTAGCTCCTG

CGCAAATCG

TATGITAATT

GGAACAGGTT

ACAGATCCTT

ACAACGGGCG

TAATTAAC

TTACTTANT

AGCGCAACCT

CAAATGCTTA

CTGGCCAAAT

AAITGGCTCG

TGGCCCGTGA

ATGGCATAGT

ATGGTTATTT

TTTCNGNAAC

GA'ITCCGATG ACGGCTTNCG

TCCCGGCTCT

AGGACCACAC

TGTCTCATAT

TCAATATGGC

AACGAATAAT

TTTGGCTCAG

CACTCATGAT

AGCGCGTGGG

AGGTOAGACT

GCAAGCAATG

AGTTTAGACA

TTTGTCCGAT

GCAGGTTCTT

GCGGCGACAT

AACATTGGTG

TGGAT1'ACAA

GGATTAGTAA

GGTCA PIA

CTTFACACCAT

AATTAATTGT

ATGAAGTGGC

CTGGTGGACG

TAGCTAATCA

TTAAAAAAGA

CTCGATrTTTC

AAAATTGGTG

AGCGCATTGG

CA'GCCAGCT

CGA.AGCGGAT

T=ACATGAA

CTATATGAAA

AGTG-AAACA-A

AAAAGCCTAT

TTATGCTGCC

TCTiGGACGAT

GGGAGATCAT

CACNAGATTI

EF071-4 (SEQ ID NO:272) F KXLMIQLALV IGLSLTIPMT AXAYTIEADP INFTYFPGSA NWSNAYVSYF VGSGGRVKQL APACQIQYGA VNLARDLAQN IGADFSLDDG TGYGIVTHDW TGVSXTGETG HYSAR

SNELIVLHES

GSLANQKAYA

ITKNWWGDHT

GNERNLGPHS

QIELARTNNA

DPYGYLARGG

LDNEVAYMKR

ATFKKDYAAY

LVKR IGTRFT EF072-1 (SEQ ID NO:273)

TAATCAATGA

TTI'CACAGC

'ITATrGTTCC

CTO-CTTCAAA

CCGTTTTATC

GAAACCGGTG

GAAGATGGAG

TATTATT'ro

GTGATTTTGC

AAAAATGAAG

CAAGGCTTTA

ATITTAGGAT

CCAGAATCAA

ACGCAAAAGC

AAAACGCACG

AAGCATTAGC

CTGATGGTCA

ATTATCGGGG

AGCTTCGTI'C

CAACAAATAG

TGGTTTCTT

'ITGAAGCGGA

CTGTI'CAAGA

AAAATGCCTA

ATCAAGGAGA

ATCAGGAA'

TTGAGGTAAA

ATGGA'ITTAC

T1'GGTTAAGT

AGAGGCATCG

ATTACCAGAA

C'I AAATGAC

TGAAGGAAAA

AAAACCGATC

TTCA'ITAGCT

AGCACCAGAA

TCCACAAGGG

TGACTTACCA

GCACATTAAC

CCGTTTGTCA

AGTGGCTGGA

GC'ITGATT

ATT-TGCGTCA

CAAGCAAGCG

CAGCAGCAAA

GTCACT1'ATC

ACGGTCCAAG

GCAGAAGATA

AGCAAAGATT

GTGGTAAAGG

CAATCGTrAA

CCACTTGAAA

TATCAGTTAA

GATAAGG-CGG

AAAACAGTTA

TCAATTAAAG

TGCTACTCGC

TTCAAGTTAC

ACACAGGGGA

AAGTCTATGA

AGGCACAGCG

AAACACAGAC

CGCAGCAACG

AAAA.AGCTAG

CGCATATTCA

AAACGGTACT

CGACTCAGAT

ATACAACGTT

CTACAGGTI'A

ACTI'ACAkA.A

TCTTTITCGGG

GTI'GCACAAA

AGAGGGA.ACG'

TGTGACGGAT

TCAATTAGCA

AATAAATGGA

AGATAAAGCC

CAACCTAGTA

TTTATATCCA

CGATAAGCAA

TCCAGCGAAT

GACACITA

CACACTGACG

CTTTGCAAAT

163 TABLE 1. Nucleotide and Aminio Acid Seqeuences of E.faecalis Genes.

CAAACAATGA

ATTAACAACG

CGTACAGGCG

GAGGCTGTTT

TATCGTTGGC

TI'TCAGTTA

AGG'ITATCTT

GAACGGACAA

CTGTGTCGTA

AAGGACAATT

GCAAGTC'rrT

TTATCGTCAA

AAAAAGAAAA

AAGGNNTTA-A

CTGAATCAAA

CGAACAGCAC

TCAAATGCGT

ACTCACGGAC

TGTCAAAGTT

AAATCAAGCG

AGCATTAGCT

AAGATGGCCA

CAGAAATTCC

CGTTACATGT

TTAGAAAAGA

AAACATACCT

GATAGTGAA.A

GGGGAATACC

AAAAAATTCA

GTACTTCTTG

TTTTACGGTG

AATCAATAA

CCGC-TGAACC

TGACT AAAAG

ATGCGAAAAT

TCAATGAAAC

CGTCTAATCA

GAAGAAATCT

GGAAAAAATT

TGACACTGCG

ACCCACA'GTT

CACCTTGCCA

AGCAAAC3-GG

AGCCZCGTGA.A

CTGCACCAAA

CTTATGCAAC

EF072-2 (SEQ 7D NO:274) MXNARWLSI CVMLLALFGF SQQALAEASQ ASVQVThHKL LFPrDGQLPEQ.QQNTGEEGTL

LQNYRGLNDV

DGVVS FSLAS

NEENAYDLPP

ES EVKVAGI<

NNEGQLVTDK

iWQKEKALAI(

RTTNSTVTCN

TYQVYDVTDP

KDSQQRDKAY

LEKTVLDKQQ

TVTTGYTLTT

HTLTKRATVR

KFTSNQAGEF

FYQLRSEGKT

LFVE.AEAPEV

GFNQGEH INY

QKHGFTLDFS

TGGKSFVYKVD

SVKGXKRWPV

VQEAQRQLAE

VKEKASNLVV

QLTTQI PANI

IKDLQNFANQ

SENAKITLPE

LLGRNLCTKR

TGATNRkPIA

ILPVQDPQGQ

LGYQEFRLSD

TMTVSYQMRL

AVF IVKNQAG

LSSESNRNSF

EDKTQTINGE

SLTHIHLYPK

KADTI'LTLLP

ERTAEPOTAI

EYLNETANGY

YGGKKFLCNE

0@ EFO72-3 (SEQ ID NO:27S)

ATTACCAGAA:

CTGCTTCAAA

CCGTTT1'ATC

GAAACCGCGM

GAAGATGGAG

TATTTAT=r

OTGATTTTGC

AAAAATGAAG

CAAGGCTTTA

ATTTTAGGAT

CCAGAATCA-A

ACGCAAAAGC

CA.AACAATGA

A ITAACAACG

CGTACAGGCG

GAGGCTGTTT

TATCCTTGGC

TTTCAGTTA

AGGTTATCTr

GAACGGACAA

CAGCAGCAAA

ATTATCGGGG

AGCTTCGTTC

CAACAAATAG

TGGTTTCTTr

TTGAAGCGGA

CTGTTCAAGA

AAAANPJCCTA

ATCAAGGAGA

ATCAGGAATT

TTGAGGTAAA

ATGGATTTAC

CTGTGTCGTA

AAGGACAATT

GCAAGTCTT

TTATCGTCAA

AAAAAGAAAA

AAGGNNTTAA

CTGAATCAAA

CGAACAGCAC

ACACAGGGGA

CTAAATGAC

TGAAGGAAAA

AAAACCGATC

'ITCATTAGCT

AGCACCAGAA

TCCACAAGGG

TGACTTACCA

GCACATTAAC

CCGITTTGTCA

AGTCCCTCGGA

GCTTGATITI

TCAAATG.CGT

ACTCACCGAC

TGTCAAAG T

AAATCAAGCG

AGCATI'AGCT

AAGATGGCCA

CAGAAAI'CC

CGTTACATGT

AGACCA.ACG

GTCACTTATC

ACGGTCCAAC

GCAGAAGATA

ACCAAAGATT

GTGGTAAAGG

CAATCGTTAA

CCACTTGAAA

TATCAGTTAA

GATAAGGCGG

AAGTCTATGA

AGGCACAGCG

AAACACAGAC

CGCAGCAACG

A.AAAAGCTAG

CGCATATTCA

A.AACGGTACT

CGACTCAGAT

ATACAACGTT

TGTGACGGAT

TCAATTAGCA

AATAAATGGA

AGATAAAGC C

CAACCTAGTA

?TTATATCCA

CGATAAGCAA

TCCAGCGAAT

GACACTTTTA

AAAACAGTI'A CTACAGGTTA CACACTGACG TCAATTA-AAG ACTTACAAAA CTTGCAAAT

TTAGAAAAGA

AAACATACCT

GATAGTGAAA~

GGGGAATACC

AAAAAATTrCA

GTACITCTTC

TTTTACGGTG

CCGCTGAACC

TGACTAAAAC

ATCCGAAAAT

TCAATGAAAC

CGTCTAATCA

GAAGAA.ATCT

GGAAAAAATT

TGACACTGCG

AGCCACAGTT

CACCTTGCCA

AGCAAACGGG

AGCCGGTGAA

CTCCACCAAA

C ITATGCAAC EF072-4 (SEQ ID NO:276) QLPEQ QQNTGEEGTL LQNYRGLNDV TYQVYDVTDP DCVVSFSLAS KDSQQRDKAY NEENAYDLPP LEKTVLDKQQ ES IEVVAGK TVTTGYTLTT NNEGQLVTDK HTLTKRATVR RWQKEKALAI( KFTSNQAGEF

RTTNSTVTC

FYQLRSEGKT

LFVEAEFAPEV

GFNQGEH INY

QKHGFTLDFS

TGGKSFVKVD

SVKGXKRWPV

VQEAQRQrAE

VKEKASNLVV

QLTTQIPANI

IKDLQNFANQ

SENAKITLPE

LLGRNLCTKR

TGATNRKP IA

ILPVQDPQGQ

LGYQEFRLSD

TM'IVSYQMRL

AVF IVKNQAG

LSS=ESNRNSF

EDKTQTINGE

SLTHIHLYPK

KADTTLTLLP

EKTAEPDTAI

EYLNETANGY

YGGKKFLCNE

164 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

EF073-1 (SEQ ID NO:277)

TAAATGAACA

ATTGCTGTCC

TTCGTTCTCT

ACGAAAGATA

TTCAGAAGTA

CGTTCAACTA

CTGTTTAAAG

ACACATTTAC

GTAAAAAACA

TCAAAATTAG

GTCGAAATGT

CAAGTAAACG

AATTAAATAC

CTAGAGAATA

ATCCAAAAAA

AAGAAGCCCA

CAATGAGCAC

AATTACGCC

AGTTAGTGAA

CAAATATCGT

AACAAACGTA

TTAAAAATGA

CGATCGCTAA

AAGACCAGCA

AAAATTACTG

TGGCTTCTflC

GAAAAATGCC

TTATCGTGAG

AGCCAAAAAA

GA'IIGACTTA

AGAACCTAAA

TGACTITAACA

TGAAAAATTA

TTATGAGGAA

AAGCAGCTM'

ATAA

ATTGGCTATA TTCTTTTAGG AGCCTTALATC

GCTTTI'GTGA

GCCGACAAAA

TTGGGGTTAT

CAAATCATAC

CGTAATGATA

AAGTTACACT

4GTAAACATT

GAAGAAAGCG

ATCGTTI'CCG

TCGCAAAAAG

TTCTGGTAGG

GCGATCAAAT

CTCCACAAGA

AATTGCAAGA

CTACGAAGGT

TAGCCAATCA

TAGAA.ATCGA

CACAAATCAT

ATGACTTAGA

CTGCAACTGA

CTTTTTAGTA

GCCTTACTTA

AATTGATT

AAACATGAAT

TTCTAAAGCT

CTITCTCTAT

ACAACACGAA

TGACCAATTG

CGATTTAGAT

GGAATCACCT

EF073-2 (SEQ ID NO:278) MNKLNTKLL I

KDKEAHYREL

FKEL.VKEPKK

KLVXNDYEE I GYILLGALI I GtLSPQEIDFF

LHLANHFLYT

VSDDLDDLDV

AVAREYGFFA

RSTMSTA.KKQ

HLPNIVDLTS

EMS IAKSSLS

FVILVGFLVF

IIQLQENIMR

KHLEIEQHEV

QKAATEESPQ

VLYRKKKNAA

STKLRAIDLR

YKDKQTYEKLE

VNEIDQQ

OKSDQMPYLT

NDTTKVSKAL

ESAQI IDQLS EF073-3 (SEQ ID NO:279) CT ATCGAAAAAA GAAAAATGCC GCCGACAAAA GCGATCAAkAT GCCTrA.-TTA ACGAAAGATA AAGAAGCCCA TTATCGTGAG TTGGGGTTAT CTCCACAAGA AA ITGATT TTCAGAACTA CAATGAGCAC AGCCAAAAAA CAAATCATAC AATI'GCAAGA AAACATGAAT CGF'rCAACTA AATTACGGGC GATTGACTTA CGTAATGATA CTACGAAGGT TTCTAAAGCT CTGTTTAAAG AGITAGTGAA AGAACCTAAA AAGTTACACT TAGCCAATCA CTTTCTCTAT ACACATTTAC CAAATATCGT TGACTTAACA AGTAAACAT1' TAGAAATCGA ACAACACGAA GTAAAAAACA AACAAACGTA TGAAAAATTA GAAGAAAGCG CACAAATCAT TACCAATTG TCAAAAITAG TTAAAAATGA TTATGAGGAA ATCGITrTCCG ATGACTTAGA CGATI'AGAT GTCCAAAP~T CCGATC(GCTAA ACAGCTTC TCGCAAkAAAC CTGCAACTGA GGAATCACCT CAAGTAAACG AAGACCAGCA AT EF073-4 (SEQ ID NO:280) YRKKKNAA DKSDQMPYLT KDKE-AIYREL GLSPQEIDFF FKELVKEPKX LHiLAN}IFLYT KLVKNDYEEI VSDOLDDLDV

RSTMSTAKKQ

HLPNIVDLTS

EMS IAKSSLS I IQLQENNR

KHLEIEQHEV

QKAATEE-SPQ

STKLRAIDLR

KNKQTYEKLE

VNEDQQ

NDTTKVSKAL

ESAQI IDQLS EF0'74-1 (SEQ ID NO:281)

TAAAGGAGT

ACCGTTCCAG

ATGGTAGATA

GGACGCGATG

GCCTACAATCG

TTCAAGCCT

CAAGGTCGCG

GGCGATGAAC

GT'TAGACA

CTCAAAAAAT

TTGGCT=TG

TCTCTGGCAA

GTITACAAACA

TCGTACCGGT

ATAACCAAAC

CAGTITrAT

AAGCCTTTC

TCGACTTAGA

GAAGCTAAA

GTTAACCCCT

AAAkAGTGTTG

AGGAACATCA

[TCCTTCATG

GGACACTCC

GGCACTTGGT

GAATGAAATC

GCAAITGGCG

AAAATAATTC

ACTCAAGC~r G [TGGATATT

GCATCACTAA

AAAAGCGATG

ETCCGACAAG

GGAGCAGATG

ATTCCTCAAG

ATTACTGCTG

CICTcTcCC

CTCCAGATGC

GGCATAACTG

ACCTTTCAGA

GCACGACACG

AAGTCGCACA

CACATATTCA

TGGAAACATA

GCGACAACCA

CCTTCTTTCA

TGCGGATACG

GGCCTCAAAA

AGTAAATCAA

GA'ITCCTACG

ATTAXATAGT

ATAGTCAAA

CGGCTTTGAT

AACCGTCATC

165 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

CCTGCTACGT

ACGATGGCAC

TCCCTAAATG

GTCTGGGTTG

TTCCTCTATN

TGAAAATAGT CAAAGAC CAT CAGAATTCCC TTATTTAAAA CTTATTATGA TTACATTGCC ATGAAGTTAT GACTTGGGTT

ATATT

TATCGAGCAC A.AGGAAAAAA TTTCATCATT CCTGGTGCCG CTTATOAAAC ATACATTACT CCACAATTAT ATAACCAAGG CCCCGACGGT GCTCAAAGCA ACGATGCTCT AAAATACGAG EF074-2 (SEQ ID NO:282) MXLKX IIPAFPLLST VAVGLWLTPT QASADAADTM VDISCKKVLV GYWHNWASKG RDGYKQGTSA SLNLSEVNQA YNVVPVSFK SOGTTRIPTF KPYNQTDTAF RQEVAQLNSQ GRAVLLALGG ADAHIQLVKG DEQAPANEII RQVTYPFDG LOIDLEQLAI TAGDNQTVIP ATLXIVKDHY RAQGKNFIIT MAPEFPYLKP GAAYETYITS LNGYYDYIAP QLYNQGGDGV WVDEVMTWVA QSNDALKYEF LYXI EF074-3 (SEQ ID NO:283) TGC TGCGGATACG

S*

S

S

ATGGTAGATA

GGACGCGATG

GCCTACAATG

TTCAAGCCTT

CAAGGTCGCG

GGCGATGAAC

GGTTTAGACA

CCTGCTACGT

ACGATGGCAC

TCCCTAAATG

GTCTGGGTTG

TTCCTCT

TCTCTGGCAA

GTTACAAACA

TCGTACCGGT

ATAACCAAAC

CAGTI'ATT

AAGCCT'ITGC

TCGACTTAGA

TGAAAATAGT

CAGAATTCCC

GTTATTATCA

ATGAAGTTAT

AAAAGTGTTG

AGGA.ACATCA

TTCCTTCATIG

GGACACTGCC

GGCACTTGGT

GAATGAAATC

GCAATTGGCG

CAAAGACCAT

TTA'ITTAAAA

TTACATTGCC

GACTTGGGTT

GTTGGATATT

GCATCACTAA

AAAAGCGATG

TCCGACAAG

GGAGCAGAM

ATTCGTCA.AG

ATTACTGCTG3

TATCCAGCAC

CCTC CCCG

CCACAATTAT

GCTCAAAGCA

GGCATAACTG GGCCTCAAAA ACCTTTCAGA AGTAAATCAA GCACGACACG GATTCCTACG AAGTCGCACA ATTAAATAGT CACATATTCA ATTAGTCAAA TGGAAACATA CGGCTTTGAT GCGACAACCA AACCGTCATC AAGGAAAAAA TTTCATCATT CTTATGAAAC ATACATTACT ATAACCAAGG CGGCGACGGT ACGATGCTCT AAAATACGAC

S.

S*SS

S

EF074-4 (SEQ ID NO:284) AADTM VDiISGKKVLV GYWHNWASKG RDGYKQGTSA SLNLSEVNQA YNVVPVSFMK SDGTTRIPTF KPYNQTDTAF RQEVAQLNSQ GRAVLLALGG ADAH IQLVKG DEQAFANEI I RQVETYGFDG LDI DLEQLAI TAG DNQTVI P ATLKIVKDHY RAQGKNFI IT MAPEFPYLKP GAAYETITS LNGYYDYIAP QLYNQGGDGV WVDEVMTWVA QSNDALKYEF LY EF075-1 (SEQ ID NO:285)

TAACCTATA-A

GGGAAGAAAA

GGAATGGGAG

GTAGAACGAT

TTATTAGGGA

ATTGTTGCAA

ACCTATGTTG

GGTGGCGCAT

TATG'TrCAA

GTGAACAATA

TICGATGGTG

TACGGCCGCC

CTI'AACAGCO

GATTT1AAGTT

GAAAAAAATC

TTTT'GCCAT

CAAAACTTTIA

CTAAAAAAAG

TTGATACAGG

CAGTI'AATCC

ATATrCCAGG CTTTrAGCAAT

TAATATGGC

ATCTGACTTT

A.ACAAGCACT

AAGAACGTCA

TAAGCAACTA

TTGATGACAT

ACAACCTGTG

TATCNTIGGA

TTGGGATGTT

TCAGGTCAAT

CGATGATGGG

TCGTGACAAG

TCAACGAAAA

GGACACAGTT

TGGTTI'AAAA

TTCTCAAGAC

CTCCTATTCA

AAGAAAAGTG

TCAAGAAATT

GAAAAAA.ATT

ATAAATTATNI

ATTATCTTGG

TCTMPATCAA

?I'AAACAATA

CGTGTCGAGC

CAAACAACCT

CAAGATAAAT

GAAAACTATT

GAATTAGTCA

GGATATGATI'

AGAATGcG'TT

ATGAAGGCA

TTAACAGcTlG GCCTTAGA T

GGAGGNAAAA

NTCTATTI'CT

TGGATAAAAC

AGGAGCCTTTr

AAGGTCGTTC

TAGTCAGTCT

TGAATCACC

TAAACATACC

ACGCGGTTGG

TTACGATI'GG

ACGAAGACCC

TCGTCCAAAA

TTTCTGATAA

ATCGCAGTGC

TATGTCAAAA

TGCAGTTGTT

CTATGAAACA

'rTCTG ITTTA

GGATACAACA

TGCTCGCGAT

CTATGCTT

TATTAATCAT

CGGAATCGAA

TAAANTCA

TAATGGTGAC

AGTCIAAGT

TATGAAGACA

CTTTGGTAAA

166 TABLE 1. Nucleotide and Armino Acid Seqeuences of E. faecalis Genes.

GTGAAACAAG ACCAACTTCA AGGTACTGGT TTTATGCAAG ATGGTGTTI'C CTATCAACGT GTGGATGAAC AAGAAI'AAC TCCTGTCCAA CAAGAGTTGA AAAATCAATT GAATACAAAA

TAA

EF075-2 (SEQ ID NO:286) MSKG KKIFAI IXG I ILXLFLAWVG MGAKLYWOVS KSMDKTYETV ERSKKSQVNL NNKEPFSVLL LGIDTCDDCR VEQGRSDTI'I VATVNPRDKQ YVDIPGQGKQ DKLNHAYAFG GASLAMDTVE NYLNIPINHY VSINMAGU(E NNNLTFSQDG YDFTIGKISL DGEQALSYSR MRYEDPNGDY GRQERQRKVI NSVSNYQEIL -iAVSDNMKTD LSFDDbM(XIA LDYRSAFGKV KQDQLQGTGF DEQELTRVQQ ELKNQLNTI(

TTLVSLARDT

LVNAVGG 1EV

EGIVQKVLSL

MQDGVSYQRV

EF075-3 (SEQ ID NO:287) ACTTTA TTGGGATGTT TCTAAATCAA TGGATAAAAC CTATGAAACA a a.

a a a a

GTAGAACGAT

TTA'ITAGGGA

ATTGTI'GCAA

ACCTATGTTG

GGTGGCGCAT

TATGTTTCAA

GTGAACAATA

TTGGATGGTG

TACGGCCGCC

CTTAACAGCG

GATTTAAGT

GTGAAACAAG

GTGGATGAAC

CTAAAAAAAG

TTGATACAGG

CAGTTAATCC

ATATTCCAGO

CTrTAGCAAT

TTAATATGGC

ATCTGACTr

AACAAGCACT

AAGAACGTCA

TAAGCAACTA

TTGATCACAT

ACCAACTTCA

AAGAATTAAC

TCAGGTCAAT

CGATGATGG

TCGTGACAAG

TCAAGGAAAA

GGACACAGT

TGGTTTAAAA

TTCTCAAGAC

CTCCTATTCA

AAGAAAAGTG

TCAAGAAAT

GAAAAAAAT

AGGTACTGGT

TCGTGTCCAA

'ITAAACAATA

CGTGTCGAGC

CAAACAACCT

CAAGATAAAT

GAAAACTATt

GAATTAGTCA

GGATATGA~TT

AGAATGCCI'r

ATTGAAGGCA

TTAACAGCTG

GCCTTAGATt

TTATGCAAG

AGGAGCCTTT

AAGGTCGTTC

TAGTCAGTCT

TGA.ATCACGC

TAAACATACC

ACGCGG'ITGG

TTACGA'FIGG

ACGAAGACCC

TCGTCCAAAA

rTTCTGATAA

ATCGCAGTGC

ATGGTGITC

TTC-TGT=rA

GGATACAACA

TGCTCGCGAT

CTATGCTTT

TATTAATCAT

CGGAATCGAA

TAAAATTCA

TAATGGTGAC

AGTCTTAAGT

TATGAAGACA

CTIGGTAAA

CTATCAACGT

CAAGAG'FTGA AAAATCAATT GAATACAAAA £F07S-4 (SEQ ID 110:288) a a a a.

KLYWDVS KSMDKTYE'IV ERSKKSQVNL NNKEPFSVLL LGIDTGDDGR YVDIPGQCKQ DKLNHAYAFG GASLAMD'IVE NNNLTFSQDG YD)FTIGKISL DGEQALSYSR NSVSNYQEIL TAVSDNMIITD LSFDDMKKIA DEQ)ELTRVQQ ELKNQLNTK

VEQGRSDTTI

NYLNIPINHY

MRYEDPNGDY

LDYRSAFGKV

VATVNPRDKQ

VS INMAGLKE

GRQERQRKVI

KQDQLQGTGF

TTLVSLARDT

LVNAVGG 1EV

EGIVQKVLSL

MQDGVSYQRV

EF076-1 (SEQ ID NO:289)

TAGAAAATAA

AGCATTGCTG

AAGGTATCTC

GGAA.ACCAAA

TTAAATGTTG

G'ITAAAGACA

AAGTTAAAAA

TAA

CAGAGGAGCT

CAG=CAAG

ATGTTTCCAA

AATTATTATC

TGGATCGTGT

ATACAGATTC

AGTGGCCTAG

GAAGGAAATG

TGTCTCTGT

TCGTTATAAA

GATTGTCGAT

GAAAGATGGC

TTTAAAAGAA

GCCATC7 rr'

AAAGCATCAA

GCAGTCATCG

GTTAAAA.AGT

GATTTATCCG

GGTTCAAAAT

CGCTTTITCA

'rITrATAAA CAAkAAATTGG

CTTCTGAAAA

TTGTAGACGA

ATGATGAATT

TAGCTGAATA

CATTTATTGA

ATAATTC=T

TATCGGTTA

AATTATTAAG

TAAATT I-AT

AGATTCTGTT

TGGCGAAAAA

AGATGCAATG

TGTITCAACA

TABLE 1. Nucleotide and Amino Acid Seqeuences of E.Jfaecalis Genes.

EF076-2 (SEQ ID NO:290) MK ASTKICIGLS IAAVASVSVA VIASEKIIKK VSHVSNRYKV KKFVDDKFDC NQKLLSTVDD LSDDELDSVL NVVDRVKDGG SKLAEYGEKV KDNTDSLKER FFTFIEDAK LKX<WPRPSFF YXNNSFVST EF076-3 (SEQ ID NO:291) CATCG CTTCTGAAAA AATTATTAAC AAGGTATCTC ATGTTCCAA TCGTITATAAA G ITAAAAAGT GGAAACCAAA AATTATTATC GATTGTCGATGA GATATCCG ITAAATGTI'G TGGATCGTCT GAAAGATGGC GCTTCAAAAT GTTAAAGACA ATACAGATTC TTTAAAAGAA CGCTT'TrCA AAGTTAAAAA AGTGGCCTAG GCCATCTTTT TTTTATAAAA

TTGTAGACGA

ATGATGAATT

TAGCTGA.ATA

CA'FrTATTGA

ATAATTCTT

TAAATTCAT

AGATTCTGTT

TGGCGAAAAA

AGATGCAATG

EF076-4 (SEQ ID NO:292) VIASEXIIKI( VSHVSNRYKV KKFVDDKFDG NQKLLSIVDD LSODELDSVL NVVDRVKDCG LKKWPRPSFF YKNNS SKLAEYGEKV KDNTDSLKER FFTFIEDAMKD EF077-1 (SEQ ID NO:293)

TAAT-GTAAAG

ACAATTATAA

GCGTATGGCA

ATI'CAAACCT

GCAACCTTAG

GGTGATTCAT

GATAACTCGC

GAGGAMA.TCA

GGCTTGGTAA

CCAATTGAAA

TTCAAAATGG

GTGAAAGAAT

CCTTTTACAC

ACACCTTTG

AITTGC=TAG

ACGATGGTCG

ACGCAAGGAC

GAATTAGTCC

ATTGTTGCTT

OTTTCTGGTG

AATTTI'GTGA

TCACGTAATG

AAAGAGACTA

GATCAAGAAT

GAATGTfl'AC

GTCATCATGG

ATTCCTATG

AAAGATGACT

GCCAAACAAT

ACCGGGATCA

ATCTTATCTG

TGAATGATGG

CAGGAGTTTT

TTATTTITAAT

TACGTGAAGG

CTGTGGGAGA

TAGAAGACTA

CACAAAAAGC

ATGTTCGCGA

AAACCGCGAC

AAAATCCTGG

TTGCTGAAAA

CTGCGCGCCG

TAGTTGCCTA

CGGAAGTCTI'

TGGCAGGGAT

AAAAATTAGC

AACTTTCTGT

GATI'GCCAGC

AT'GCCAGAAA

CTGGCGTGAA

CACAAGAGTC

GCACATATTT

TGGAAAAATT

CCGTI'GCAGA

CACAAGATAA

TAGGAGATGG

GT'CTCATGG

TAAGTAAAGT

CTGTATTAAT

TTCCGGCGCT

CTTTGCGTGC

GAGAGAAAAA

GGCATTATTA

AACAGGTTCT

AAAATATGGT

ATACTGGGCC

TGCCGCTGGA

TCATCGCTTG

TGAATTAGTA

ATCAACAGTC

GGATGAATTA

AACTGTAGCA

TCCAGCTCAT

CCTAATTGCA

AGTTGTTGCT

GGGTCGTTCA

TTCTGCAAAA

TGATCAAGTC

AAGCGTGG;A

GCAAGATGTC

GGCA'T7TG

TCAAGAAACT

AGGCCGAATT

ACACCAATI'A

AACCATTGCT

ATTAACTATT

TGTAAATGAT

AGCTACTGCG

CAGCCAACCG

CGGAATTTT

AATCGCGGCT

TCGTCGAATT

GAGATGAAGC

TTTGAATTTA

GTAATGGCGT

GTCGATATTT

AGT'rTGAT GA

AAAGCTAACC

AATGGCGAAA

GTAAAACCAG

GATGAATCTT

ATGTCGGGTT

GACAGTCAAT

TTTGTACGTT

GGTGTI'CTT

TCGCCGTGTC

AGTCGTCATG

ACGATTGCGT

CAACCAATCA

CAAGAATCAA

CCATTAAAAA

GATGGTGCTG

GAAAAAA'TG

ACTITTACAG

CATCTTCAAC

GCAGAAGTAG

CTAAAAGAAT

GCACCTI'CGC

GCTAGTGAAA

GTCA.ATTG

ATCTGCGT

ATGCTACAAG

GGCCAGTAA

ATGTAACAAA.

'ITTTACATCA

TAATGATGTI'

TAGCGAT1'AC ~TTTrAATI'AT

AAGAGCTGAA

AT7TAGAAGA

GGGAACTAGT

CA'FIAACAGG

CCGTGAATGG

ATCAAACAAT

TAGCAGATCG

GGI=GTTC

CTTTAAITCT

GGGTCGTTAT

TTCATAAAAC

ATGCTGGAAT

CTCATATITT

ATATTACAGA

AGATACGGGT

ATAAAACGAC

ACACTGTACG

GAATITI'AAT

GAATTACCGA

TGCCTAAAGA

TTGCTGCTGC

CTGCTGACGT

CCCAAGATAC

TACTAAGTT

AAGTCGTGGA

ATTrGGGGATT

CCCGAATTG

CTGGGAAATC

CGCTATCGTT

GTITGACTGGT

CTCATTA'ITG

TGTTTCTGTT

TCCAGTI'GAT

AGAATCAAAA

TGACGGCTCT

TGTGAACTTA

CTATCCGGTA

AAAAAGTCCG

ATCTGCCCCA

TAAATCGGGA

AGGCACGATT

AACTGCTGCT

AGCTAGATCA

TCTAGCGGAA

AGGTAAAA-AG

TATTCATATT

CCCAGAAGCA

GCTGACGGGG

AGTACATGCG

AAATCATCCA

AGACGTAGGT

TGTTATTTTA

CATGAAAATT

AATIGCTAGT

CACTGTGTCA

168 TABLE 1. Nucleotidc and Amino Acid Seqeuences of E.f[aecalis Genes.

EF077-2 (SEQ ID NO:294)

MKHVTKLGIT

QTLREGKYGV

NS PQKAHRLN

IEKNPGDELM

FTLVAYL lAG

MVEKLASAKT

VAYARKQDVP

RNGTYLGRIT

CLPQDKLTIL

ODLSKVSQAJ

LSALRARRIG

IITGVLALLF

DILAITAIVA

GENLEDVSVE

SGSVNGDGSL

VAWFVSKSPT

IAFDKTGTIT

LKNITOLAEV

FTDTVRPEA-K

KELPKENH-PV

EIAQDTKA

EFILHQPNWA

TLAVGEYWAS

EINVGDELVV

KI4VAEKTVAD

RFAEIVAS

QGQLSVDQVQ

SGAGVKAFVD

YGIILITGSV

LMILIMLTGG

KPCELVPVDG

SQYQTIVWN

PCPLILSAPI

PINAGITAAE

GAEIRVGKKN

MALMMFXEMI

DSLEDYAAGK

LVKTCTSTVD

KESAARPAHF

ALVAGMGRSS

LVGLAASVEQ

FVTQESQETE

QESVAETIAA

AMGARGATAA

GI IPALIGAM

ANQELKSLLD

ES SLTGESK(P

VRLADRYAVP

RHGVVIKSGT

ESSHILARS I

KIDKTTIHIS

EVGITEVHGE

SETADVVILK

LQEVVDTVS I ETMEKLjHQLHLQRILMLTGD IMVGDGVNDA PSLAA.ADIC I KQSVLIGIFI CVLLMLIAST EF077-3 (SEQ ID NO:295) TCA GCCGAATTGG

S

S.

SW

S

We 55

S

S@

0* S

S

5 0

GCGTATGGCA

ATTCAAACCT

GCAACCTTAG

GGTGATTCAT

GATAACTCC

GAGGAAATCA

GGCTTGGTAA

CCAATTGAAA

TTGAAAATGG

GTGAAAGAAT

CC'=TTACAC

ACACGTTTTG

ArI'GCTTTAG

ACCATGGTCG

ACGCAAGGAC

GAATTAGTGG

ATTCTTGCTI'

G=TCTGGTG

AATITTrGTGA

TCACGTAATG

AAAGAGACTA

GATCAAGAAT

GAATGTIAC

GTCATCAM1G A ITGCTATGG

AAAGATGACT

GCCAAACAAT

ACCGGGATCA

ATCTTATCTG

TI'ATTTTAAT AACAGGTTCT GTAATGGCGT TACGTGAAGG AAAATATGGT GTCGATATTT CTGTGGGAGA ATACTGGGCC AGTTTGATCA TAGAAGACTA TGCCGCTGGA AAAGCTAACC CACAAAAAGC TCATCGCTTG AATGGCGAA.A ATGTTGGCGA TGAATI'AGTA GTAAA.ACCAG AA.ACCGGGAC ATCAACAGTC GATGAATCTT AAAATCCTGG GGATGAATTA ATCGTCGGGTT TTGCTGAAAA AACTGTAGCA GACAGTCAAT CTGCGCCGCG TCCAGCTCAT TTTGTACdTT TAGTTGCCTA CCTAATTSCA GGTGTTGCIT CGGAAGTCTT AGTTGTTGCT TCGCCGTGTC TGGCAGGGAT GGGTCGTI'CA AGTCGTCATG AAAAATITACC TICTGCAAAA ACGA ETGCGT AACTITCTGT TGATCAAGTC CAACCAATCA GATTGGCAGC AAGCGTGGAA CAAGAATCAA ATGCCAGAAA GCAAGATGTC CCATrAAAAA CTGGCGTGAA GGCA7=TTG GATGGTGCTG CACAAGAGTC TCAAGAAACT GAAAAAATTG CCACATATTT AGGCCGAATI ACTTTTACAG TGGAAAAATT ACACCAATTA CATCTTCAAC CCG ITGCAGA AACGATTGCT GCAGAAGTAG CACAAGATAA ATTAACTATr CTAAAAGMAT TAGGAGATG3G TGTAAATGAT GCACCTrCC GTGCTCATGG AGCTACTGCG GCTAGTGAAA TAAGTAAAGT CAGCCAAGCG GTCGAAATTG CTGTATTAAT CGGAATTTI ATCTCCTT 'ITCCGGCGCT AATCGGGGCT ATGCTACAAG CT'FrGCGTGC TCGTCGAAIT GCCC TAATGATGTT CTGGGAAATG TAGCGATTAC CGCTATCGTT TTTTAATTAT GTTGACTGGT AAGAGCTGAA GTCATTATTG ATI'TAGAAGA TGTTTCTGTT GGGAACTAGT TCCAGTTGAT CATTAACAGG AGAATCAAAA CCGTGAATrCG TGACGGCTCT ATCAAACAAT TGTGAACITA TAGCAGATCG CTATGCGGTA GTrTG TrTC AAAAAGTCCG CTTTATTCT ATCTGCCCCA GGGTCGTTAT TAAATCGGGA TTGATAAA.AC AGGCACAT ATGCTGGAAT AACTGCTGCT GTCATAITI= AGCTAGATCA ATAM~CAGA TCTAGCGGAA AGATACGGGT AGGTAAA.AAG ATAAAACGAC TA'ITCATT ACACTGTACG CCCAGAAGCA GAA=I'AAT GCTGACGCGrG GAATTACCGA AGTACATGGG TGCCTAAAGA AAATCATCCA 'ITGCTGCTGC AGACGTAGT CTG-CTGACGT TGTTATTTTA CCCAAGATAC CATGAAAATT TACTAATrTI' AATTGC TAC-T AAGTCGTGGA CACTGTGTCA see* .00.

*0e EF077-4 (SEQ ID NO:296) QPNWA YGIILITCGSV MALMMFWEMI QTLREGKYGV DILAITAIVA TLAVGEYWAS LMILIMLTGG DSLEDYAAGK ANQELKSLLD NSPQKAHRLN GENLEDVSVE EINVGDELVV KPGELVPVDG LVKTGTSTVD ESSLTGESKP I EKNPGDELM SGSVNGDGSL KMVAEKTVAD SQYQTIVNLV KESAARPAMF VRLADRYAVP 169 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genrs.

FTLVAYLIAG

MVEKLASAKT

VAYARKQDVP

RNGTYLGRIT

CLPQDKLTIL

DDLSKVSQAV

LSALRARRIG

VAWFVSKS PT

IAFDKTGTIT

LKNITDLAEV

FTD'IVRPEA(

KELPKENHPV

EIAQDTflIA

RFAEVLVVAS

QGQLSVDQVQ

SGAGVYAFVD

ETMEKLHQLH

IMVGDGVNDA

KQSVLIGIFI

PCPLILSAPI

P INAG ITAAE GAE IRVGKXN

LQRILMLTGD

PSLAAADVG I CVLLKL lAST

ALVAGMGRSS

LVGLAASVEQ

FVTQESQETE

QESVAETIAA

AMGAH'GATA.A

GIl PALIGAM

RHGVVIKSGT

ESSH ILARS I K IDKTT I HIS

EVGITEVHGE

SETADVV ILK LQEVVDTVS I EF079-1 (SEQ ID NO:297) TAATTTCTAG CATCACCGAA

CCCAGGCTCT

ATCATTGATG

TTTGTTIAGCG

AAAGCAAGCC

AACCAAGAAT

AAA.ACAACGA

ATI'CCAAAAA

AAAGGAAGCT

GTCATTTCAG

AAAAAAGGCG

CAAATAAAAA

CTCGTCACTT

GGACATCGTA

CAACAAAATT

ITCATTATCT

CATGCTTTAT

GTTTTATGAT

ATGCATTAAA

AAGAAAACAC

TAGCGAAAAA

AAAAACCAGA

TAA.ATGTCCG

CCTTGTTAGA

GCCATCGTGG

ATGAATTTTA

CCGTTGAACC

TATTAACTTG

TCCCATATCA

TACTATTATG

GGTACAAGCG

GAAATTTTTA

TTTAAGGAG

TCTF1'TACTG

TAACTATCTG

CAAAGAAATG

AGGCAGCAAT

CAAATCCTAT

TTTACCAA ET

AGGAACCTCC

TCTCCCTCAA

TATCGAAGTC

AACTGATACA

CACACCGTAT

ACCAGAAAAA

GACATTACTI

ACGGAAAAAG

GAAAAACAAA

GAAGCAATGA

ATTATTGGAA

GATCAACAAA

GCTGAACTTC

CCTGGATTAG

TTTGAAAGTC

T1'GATAAAA

TATCCTACAG

GCCAAATTAT

AATGGGA.AGA

AAAGATTTAC

ATGATAAACA

GCAGCAG;CGG

TTAATTGCCT

ACGACCAGAA

GAGCCT.GGGC CAATCACTGT AGTCAAAAAA GAAACGTCGT TAGGTGCATT TGCGTATCCT TI'ATCGCTCA ITATCAAGCA AAGAAAAAAT GGAAAAGAAA ATCCTTrTC TGAAACGCA ATACGATTGG TGTTTTAACC CGAkATGCATT GCTATTGGAA GTGGTACGAA TACACATGCG TTACAGATTi GCCAGAATTA CGCTTCCTTA TCAAGTAGAT ACAI'GAGTC TGGCCAAGAT GTCATCGGTTI ATTAGTTCGA GGATGAAAA AGTGGCACAA GTGCGTTAkAT TATTAGCGGC AACCAAAGTA G 0O 0 0S

S@

S 0

S.

0S S S S0 56 0 000 S

S.

0 0

S

S

*0SSeS 0 5*50 0 EF079-2 (SEQ ID NO:298) MKSKXRRI IDGFMI LLI IGIGAFAYPF VSDALNUYLD QQIIAHYQAK ASQENTKEMA ITKKPDKSYF ESHTIGVLTI PKINVRLPIF ISGHRGLPQA KLFTDLPELK KGDEMYIEVN VTIJLTCTPYM INSHRLLVRG HRIPYQPEKA IIWYKRRKKT TRKPK

ELQEIKEKKN

DKTNALLLEK

GKTLAYQVDQ

AAGMKKVAQQ

QELAKKGSNP

GSSLLEGTSY

IKTVEPTDTK

QNLLLWTLLL

GLDPFSETQK

PTGGTNTHAV

DLHIESGQDL

IACALIISGF

EF079-3 (SEQ ID NO:299) @55.

0 0@ 0 00

S.

TCCT

TI'TGTTAGCG

AAAGCAAGCC

AACCAAGAAT

AAAACAACGA

ATTCCAAAAA

AAAGGAAGCT

GTCATTTCAG

AAAAAAGGCG

CAAATAAAAA

CTCGTCACTT

GGACATCGTA

CAACAAAArr TTCATrrATCT

ATGCATI'AAA

AAGAAAACAC

TAGCGAAAAA

AAAAACCAGA

TAAATGTCCG

CCTTGTTAGA

CCCATCGTGG

ATGAATTTTA

CCGTTGAACC

TATTA.ACTTG

TCCCATATCA

TACTA'FrATG GGTACAkAGCG

TAACTATCTG

CAAAGAAATG

AGGCAGCAAT

CAAATCCTAT

TTACCAATT

AGGAACCTCC

TCTCCCTCAA

TATCGAAGTC

AACTGATACA

CACACCGTAT

ACCAGAAAAA

GACATI'ACTT

ACGGAAAAAG

GATCAACAAA

GCTGAACTI'C

CCTGGATTAG

TTTGAAAGTC

TTTGATAAAA

TATCCTACAG

GCCAAATTAT

AATGGGAAGA

AAAGATTrTAC

ATGATAAACA

GCAGCAGCGG

TTAATTGCCT

ACGACCAGAA

TI'ATCGCTCA

AAGAAAAAAT

ATCCTTI'TC

ATACGATTG

CGAATGCATT

GTGGTACGAA

TTACAGATTT

CGCILMCTTA

ACATTGAGTC

GTCATCGTT

GGATGAAAAA

GTGCGTTAAT

AACCAA

TTATCAAGCA

GGAAAAGAAA

TGAAACGCAA

TGTTI'TAACC

GCTATTGGAA

TACACATGCG

GCCAGAATTA

TCAAGTAGAT

T'GGCCAAGAT

ATTAGTTCGA

AGTGGCACAA

TA'ITAGCGGC

EF079-4 (SEQ ID NO:300) 1 170 TABLE 1. Nucleofide and Amino Acid Seqeuences of E. faecalis Genes.

VSDALNNYLD

TTKKPDKSYF

I SGH-RGLPQA

VTLLTCTPYM

IIWYXRRKT

QQIIAHYQAK ASQENTKEMA

ELQEK.\EKKN

ESHTIGVLTI PKINVRLPIF

DKTNALLIJEK

KLFTDLPELK KGDEFYIEVN

GKTLAYQVDQ

INSHRLLVRG HRIPYQPEKA

AAGM(KVAQQ

TRKP

QELAKXGSNP

GSSLLEGTSY

IKTVEPTDTK

QNLLLWTLLL

GLDPFSETQ<

PTGGTNTHAV

DLH IESGQDL IACALI ISGF EF080-1 (SEQ ID NO:301)

TAGTTACACT

TTTATTAGGA

CTI'ACC E1G

CAATTACTGA

AAAACAAAAG

GA.ATATGCAG

CTCATTGATA

ATGACAGATC,

AATTATTTCG

GTTCCTIGGGG

ATTACCCCGC

TTAGGCATTA

GAAAAAACAA

ACCTCGTA

ACCCACTCAA

CGTTTAGGGC

GGTTATTTCC

GCCTTIGCCCT

CACCTGATCA

CCTCTGTCTT

ATCATTATCT

TCGACTTACG

CACGAA'ITGA

CGGCTGCTCA

GCCACTATCG

TGGAAATGGT

ATATTTCTAA

CTTTAAACTT

TTCCTAAAAA

CTGGCGGCGG

TAGCAACGTT-AGGCATT

TTC

TATGAAAAAA

ACCCG ET1TCG

AATCAACCAA

TATTGTAACC

TTAAATAAA

GAAAATCTAC

TGATACCTTA

AACTTGTT

TGTGGACAGC

AATTGCT'I=

ATATCAATTA

AACCTCCCGC

CAATGGCGGC

CACATTCGGT

GCGG.CTGAAA

CTAAAGCAAG

ACAAATAATA

GT~rGGCAACG

ATCTCTACTT

GATAAAATAT

CAGGAAACTC

GAAACAGGTA

OCTGCTGCGC

AAATTTCAA

ACAGATCAGT

AGTGGCGGAA

GGCGGCGGTC

GCTGGACTCT

CTA=TTTT

ATTCAATTGA

AdATACAACC

ATACCTATGG

ACCAAAATGC

CT'GGAAACAT

GGGATAATAT

AAGCATTTGT

AAATCACTCG

TGATACTCAG

GTTATCAATA

TAACCAACTC

TGCCCGGTGG

GAAGTTTTTA

TAGCACTCTT

CCTAATACTT

TGATGGCGCA

TTTAGAAGAA

CGATGAACAA

GATTCT1TTTT

GATTGATTAT

GAGTCAAGGA

TAATAAAGGG

TATAA-AGTC

T1'TGGTCTTC

TCCCTTTAGG

?rI'CATCACT

TGTAGCACC

G

EF080-2 (SEQ ID NO:302) MKKR LLPIFFLILL TFGLALPVSA AENSIDDGAQ LLTPDQINQL KQEIQPLEEI( TXASVFIVTT

NNNTYGDEQE

IDMDLRKIYI STSGNMIDYM TDARIDDTLD

KIWDNMSQGN

PGGHYRVDSE TGKITRYKVI TPLEMVIAFA

AALILSLVFL

KTTLNLTSRT DQLTNSFI'IT RRIPKNNGGS GGMGGGGST

YADHYLLNKV

YFAAAQTFVQ

GINI SKYQLK HiSTGGGTFGG

GKDQNAILFL

ETQAFVNKGV

FSSYQYPFRE

GRSF

EFO80-3 (SEQ ID NO:3 03) GGCTGAAA ATTCAATI'GA

TGATGGCGCA

CAA'ITACTGA CACCTGATCA AATCAACCAA AAAACAAAAG CCTCTGTCTT1 TATTGTAACC GAATATGCAG ATCATTATCT TTTAAATAAA CTCATTGATA TGGACT1'ACG- GAAAATCTAC ATGACAGATG CACGAA'TTGA TGATACCTTA AATTATITCG CGGCTGCTCA AACCT1'IGTT GTCCTGGGG GGCACTATCG TGTGGACAGC ATI'ACCCCGC TGGAAATGGT AATGCTIT TrTAGGCATTA ATATTTCTAA

ATATCAATTA

GAAAAAACAA cTTTAMC AACCTCCCGC ACGCGTCGTA rPTCCTAAAAA CAATGGCGG-C ACCCACTCAA CTCGCGGCGG CACAT1'CGGT

CTAAAGCAAG

ACAAATAATA

GTTGGCA.AGG

ATCTCTACT

GATAAAATAT

CAGGAAACTC

GAAACAGGTA

GCTGCTGCGC

AAATTCAA

ACAGATCAGT

AGTGCGGAA

GGCGGCGGTC

AGATACAACC

ATACCTATGG

ACCAAAATGc

CTGGAAACAT

GGGATAATAT

AAGCATTTGT

AA.ATCACTCG

TGATACTCAG

GTTATCAATA

TAACCAACTC

TGGGCGGT'GG

GAAGT

T1'TAGAAGAA

CGATGAACAA

GA LTCTT'ITTT

GATTCGATTAT

GAGTCAAGGA

TAATAAAGG

TI'ATAAAGTC

TTTGGTCTTC

TCCCTTI'AGG

TTT.LCATCACT

TGGTAGCACC

EF080-4 (SEQ ID NO:304)

AENSIDOGAQ

LLTPDQINQL KQEIQPLEEX TKASVFIVTT NNNTYGDEQE YADHYLLNKV GKD)QNAILFL TA13LE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

IDMDLRKIYI

PGGHYRVDSE

KTTLNLTSR-T

STSGNMIDYM

TGKITRYKVI

DQLTNSFITT

TDARIDDTLD

TPLEMVIAFA

RRI PKNNGGS

KIWDNMSQGN

AALILSLVFL

GGMGGGGSTT

YFAAAQ77FVQ

GINISKYQLK

HSTGGGTFGG

ETQAFVNKGV

FSSYQYPFRE

GGRS

EF081-1 (SEQ ID NO:305)

TGAATGGAAC

TCTGCCTTG

GCCAAACAAG

AAACAGGAAT

TATGATACTA

GGAGTCAAAG

TATAAATTAT

ATCGCCAAAA

ATGTCAGGAA

GATCGTAATG

GGCAAACTCG

GGCGTTTCI

TTTGTACCAA

AAAGATACAC

GAAGCAATCG

ITCTAATCGC

AAT1'AGCCGA TT13ATAAGTT

AATCTGTTGT

CTAGTGATGT

CGATGAGCAC

AAGGCGATAC

ATGATAAAAT

GTAGTGGGCT

GTTCTGGCGC

TTGATGAAAT

TCACAGTCGC

AGTCACGTI'A

TAATAAAAAA

TGCTGCCGGT

AGCGAAGAAA

ACCGTCCGTT

TGAAAAATAC

TCAAGTCAAA

rCCTTAGGC

CTACCAAATC

TTCGATTCAA

AGCAATTAAC

AGAAAAAACA

CAATCAAAAG

TTCTGAACCA

TTATCCGCTG

TCTTCAAAA.A

GGCGGGTAT

ACAGCTACTA

GTTCAAGAAG

C-AAGCAATTT

AAGGCGAAAG

GAAATGAAAG

GCTTGGA.AGC

GTAGATAATG

AAAGTGTTrG

GCCAATATCA

TTAAGCCAAG

GTGACAGAAT

GGGGAAGCAN

AACCACTTAT

ATGCTLTATAG

CATTTTTAAA

CTAGC'ITAAA

ATCAGGGAT

ACAATCAATA

ATTTGTCTTA

CATCTtTAAT

CCAAACGTGG

ACCAAGTGGG

AAGCTTTTAG

GATGGG'TCCA

TACCAACAGG

TGCGCAATTA

TCTTGGTGTT

TCAATGGCAA

CGTATTGTCA

GAAAAATI-GC

TCAACCAGAA

CACATTTACC

TCAATCAAGT

FTTCCAGAT

AGAAATTGTC

CGTAGTGCCT

TGATAAATTC

AGCAGACTCC

GGCTGCGACA

EF081-2 (SEQ ID NO:306)

MERSNRNKKS

QEFDKLPSVV

KLSMSTPLGE

RNCSCLAINK

VP ITVASEPV

SKKPLILGVS

QEASLKKNGY

MXDLSYQSS I

VFDEVGVVPG

TELPTGAATK

AILVL IAAAGG

DTKSVVEKYQ

AKDTYQ IA

KLGSGAEKTA

DTESRYYPLG

GYYAYSQWQA

AIYSGIQAEG

WKPSL IFPDM

NIKAFSDKFG

EAXRN

KQELAF.AKKT

VKASDVQVKK

SGNDKIS IQV

VSVDEINQKL

ATTFLNVLSK

AKDNQYTFTY

DNAKRGEIVD

SQGWVQADSF

0 .0 0000 0 00.

EFO81-3 (SEQ ID NO:307) T GGCGGGTATI' ATGCTTATAG TCAATGCAA

GCCAAACAAG

AAACAGGAAT

TATGATACTA

GGAGTCAAAC

TATAAATI'AT

ATCGCCAAAA

ATGTCAGGAA

GATCGTAATG

GGCAAACTCG

GGCGITTCTG

TTGTACCAA

AAAGATACAG

AATTAGCCGA

TI'GATAAGTT

AATCTGTTGT

CTAGTGATGT

CGATGAGCAC

AAGGCGATAC

ATGATAAAAT

GTAGTGGGCT

GTTCTGGCGC

G-ATGAAAT

TCACAGTCGC

AGTCACGTTA

AGCGAAGAAA

ACCGTCCGTT

TGAAAAATAC

TCAAGTCAAA

GCCTTTAGGC

CTACCAAATC

TT-CGATTCA-A

AGcAATI'AAC

AGAAAAAACA

CAATCAAAAG

TTCTGAACCA

TTATCCGCTG

ACAGCTACTA

GTTCAAGAAG

CAAGCAATT

AAGGCGAAAG

GAA.ATGAAAG

GCTI'CGAACC

GTAGATAATG

AA.AGTGTMT

GCCAATATCA

TTAACCCAAG

GTGACAGAAT

GGGG

cAT'TTIAAA CGTATTGTCA CTAGCTTAAA GAAAAATGC ATTCAGGGAT TCAAGCAGAA ACAATCAATA CACAVITACC ATTT.-TCTTA TCAATCAAGT CATCITI'AAT TTTTCCAGAT CCAAACGTGG AGAAATTGTC ACGAAGTGGG

CGTAGTGCCT

AAGCT TTAG TCATAAATI'C GATCGGTCCA AGCAGACTCC TACCAACAGG

GGCTGCGACA

EFO81-4 (SEQ ID NO:308) G GYYAYSQWQA KQELAEAXXT ATTFLNVLSK QEFDKLPSVV QE-ASLKKNGY DTKSWVEXYQ AIYSGIQAEG vKASDVQVKK AKDNQYTFTY KLSMSTPLGE MKDLSYQSSI AKXGDTYQIA WKPSLIFPDM SGNDKISIQV DNAKRGEIVD RNGSGLAINK VFDEVGVVPG KLCSGAEKTA N~IKAFSDKFG VSVDEINQKL SQGWVQADSF VPITVASEPV TELPTGAATK DTESRYYPLG 172 TABLE 1. Nucleotidc and Amino Acid Seqeuences of E. faecalis Genes.

EF082-1 (SEQ ID NO:309)

TAAAAAATGA

CTT'AAATA

ATLTACGTTITG

CCTG.TGACAC

GACTATG~

TCGGCACAAC

GTTCAAGTAA

GCGCAATTTG

TAGCAG'CAA

ACCTTGGATC

ATGGGCACTT

ACTGTTCCTG

ATT'TCGATG

AAAAGATCGT

GTTCAAAAGT

CTCTTGATAA

CTAATCCTGC

CAAATATCCA

TGGATCAAGT

CTCACAAACG

CTACAAGTGA

CACCCAATI'C

CAACTGCGC

GGACATTAGC

CGACAACGAA

ATACACCACT

GCGCATTTCA

TGAAGCAGCT

TACTGTCACG

TGATCCTCAT

TT1TTGGATCA

GCAAAATAGT

TGGTCTAAAT

TTCAACACCC

A.ACACAGTT-A

CGCCAC7I'CT

TFTTGTAGC

AAAAGTTGCA

'ITAA

AGCATTTTGT

CAAGTCGCI1T

CCACCTGTCA

CAACCTGGTA

AAACAAATTC

ACTCGCCATT

CTTGGCTGGA

GCTGTTTTGG

CmCTTTGG CC Tr.TrGCGA

GGANCGACCG

GCTAAACAAT

TCGTTGCTAC

CTATTCAATC

ACCCGACGAA

CAGCCGGACC

AAGCCGGAAC

TAA ETIAGCGT

AATTATCAGT

ATAATGCATC

CGCCA'ITAAC

CTGCCGCTCT

CTqCTCAAGG AtkAAACAAC

GCCTCTTATG

CAACGCTGAT

CCCTTCTCAG

CCTTAGTAT'

AGCGATCTAT

GCCAAACTAT

TAAACAGAGT

C ETGACCT

GGTCCCAGTA

'TCAACAGGA

CATTCAATTA

GCTTACTTGG

EF082-2 (SEQ ID NO:310) M4KKIVRISS ILFVATPULL LNSSKVEAAQ VASIQSNADI TFALDNTVTP PVNPTNPSQP VTPNPADPHQ PGTAGPLSID YVSNIHFGSK QIQAGTAIYS AQLDQVQNST GDLISVPNYV QVTDKRGL 'NL GWKLSVKQSA QFATSDSTPA VLDNASLTFL AATPNSTQLL SLAPLTVPVT LDPTGAATSP VATAALSTGM GTWTL.AFGSG XTAAQGIQLT VPATTKKVAA KQYKTTLTWI

LDDTPL

EF082-3 (SEQ ID NO:311) AGCT CAAGTCGCTT CTATTCAATC CAACGCTGAT 0 0 0.

0 00* 0* 0.

000 t

ATTACGTTTG

CCMTGACAC

GACTATGTTT

TCGGCACAAC

GTTCAAGTAA

GCGCAATTTG

TTAGCAGCAA

ACCTTGGATC

ATGGGCACTT

ACTGTITCCTG

CTCTI'GATAA

CTAATCCTGC

CAAATATCCA

TGGATCAAGT

CTGAZ:XA.ACG

CTACAAGTGA

CACCCAATTC

CAACTGGTGC

GGACAITAGC

CGACAACGAA

TACTCTCACG

TGATCCTCAT

=FTGGATCA

GCAAAATAGT

TGGTCTAAAT

TTCAACACCC

AACACAGTTA

CGCCACTTCT

TTIrTGGTAGC

AAAAGTITGCA

CCACCTGTCA

CAACCTGGTA

AAACAAATTC

ACTCGCGATT

CTTGGCTGGA

GCTGTTTTGG

CTTTCTTG

CCTGTGGCGA

GGANCGACCG

GCTAAACAAT

ACCCGACGAA

CAGCCGGACC

AAGCCGGAAC

TAATTAGCGT

AATTATCAGT

ATAATGCATC

CGCCATTAAC

CTGCCGCTCT

CTGCTCAAGG

ATAAA.ACAAC

CCCTI'CTCAG

CCTTAGTATT

AGCGATCTAT

GCCAAACTAT

TAAACAGAGT

CTTGACCTTT

GGTCCCAGTA

TTCAACAGGA

CATTCAA'ITA

GCTTACTTGG

ATT =GATG ATACACCACT E'.OB02-4 (SEQ ID NO:312) AQ VASIQSNADI TFALDNTVTP PVNPTNPSQP VTPNPADPHQ PGTAGPLSID X'VSNIHFGS( QIQAGTAIYS AQLDQVQNST GDLISVPNY-V QVTDKRGLNL GWKLSVKQSA QFATSDSTPA VLDNASLTFL AATPNSTQLL SLAPLTVPVT LDPTGAATS P VATAALSTCN GTWTL.AFGSG XTAAQGIQLT VPA'ITKKVAA KQYKTTLTWI

LDDTP

EF083-1 (SEQ ID NO:313) TAA'TrTAAAA

GCTCTGTTTT

GCGATTTATC

CAGA=TGAG

GCAAGTGCTA

GACAAGGAGA

TTATCCCTAC

AAGGTGAACA

GCTACAATC

rrGCCCAAGG

AATAAAAATG

AGCTATG=T

AGGTCGCTTT

TAGCGGTATT

TAAACGTGCG

AAAAAGAAAA

CCCGCAAAAG

CGCTATGCAC

TATGAACAAT

CATACCTATA

T'ITTAGCAGG

GAGACCAAGG

ATGATAAATTr

ACACATATAA

TTG-GTATGA

AGCGCTTGTC

TGTGGATTGG

CGCTATTrCC AACGCAAGTrG

CACTTGGGGA

173 TABLE 1. Nucleotide and Amino Acid Seqeuerices of E. faecalis Genes.

AACATGGACA

AATTCCATCG

GGAGGATATG

ATGCGCAGAA

CTAAATCAT'-

GCGTATCCTA

GGTATTGGTA

GATTTAACAG

CCAGCAACAG

GATACCGTCA

TGGGTAAAAG

GGTATC'ITGT

CCTGATAAGC

GCTTATCAAT

AATCTTATTT

ACGGTTAAAT

GCTTTAGCTG

TTGCGAAAAC

TTGCATTAGA

TAAGTTCAGA

TCAAACAGGC

TAAACTATCA

TCGATGGTGT

TTTGGCAATT

GAATTACGGA

ATGCAGGCGA

AAGTGAAATT

GAAACAGCTA

CATGGATTAG

AACCAGAAGC

ATGGAACAGA

ATCCTGGTCA

ACGGCGATAA

CATTAA-ACGG

AACAATGGAT

TACTTTTTGC

TTTTGAACAT GGAGCGTTrGG TGCCGAAAAA GCAGCAAATA TGCCTATACT CCAATGTATT ACAAATCATC

AAAGAGTTTC

GTCACCATAT

CCATMTATG

CACATCCGCT TATAM3CAG TAGTGGTTAT

ACAGATACCA

AGAAAT~rAA

AAA.ATACCTA

TAATGTAGAT GCTTGGGCAA

CAAAGTGCAA-GA.AGTAACTC

CAA.AGGTGAT AiTTCAATTAT G-ACTCATGTG

GTACAATACG

CTATCAAACG

TTGGCGGCAT

AGTTTTG;AAA CTCAATGGAT TTTATC-TAGT

ATTGCAGCA-A

ATTAGCAAAT

CCTAACTTIGA

:ACGTATTICA

:TAGTGTTCC

CAGAGACAAT

PICAGCTATAA

CTAACTC TI'

CTATTTCCC

GTGG'TTTAGA

ATAAACCAGA

ATTCTGATiGT

CTGGGGAAGC

GAAGCAGAGT

TGCCAGACGC

GAGAA.ACATT

TAAXATGGATT

CGGCA.ACAAG

AACTTGGCAC

TTTATCCAGG

ACGCCTAA-A

AGATIGGATAT

TTTGTACGGT

GCCATTTACA

ATGGATTGCT

ALAGCATGGAT

TGGTAkACGTA

A.ACGGATACG

TAAAGT1'GGC

TATTCCGCAA

ATTGCTTGAA

AACAGTCGTC

ATCAAGTAT

GGCTAATCCA

TAATGTCTAC

TACTTATCAA

TCAAAICATTG

AATTATTAA

EF083-2 (SEQ ID NO:314) MK KKILAGALVA LFFMPTAMFA AKGDQGVDWA IYQGE-QGRFG

YAHDKFAIAQ

IGGYNASGIY EQYTYKTQVA SAIAQGKRAH TYIWYDTJGN MOIAXITFMfY

FLPRIQTPKN

SIVALDFEHG ALASVPDGYG GYVSSDAEKA ANTETILYGM RRIKQAGYTP

MYYSYKPFTL

NHVNYQQIIK EFPNSLWIAA YPIDGVSPYP LYAYFPSMDG IGIWQFTSAY

IAGGLDGNVD

LTGITDSGYT DTNKPETDTP ATDAGEEIEK IPNSOVKVGD TVKVKFNVDA

WATCEAIPQW

VKGNSYKVQE VTGSRVLLEG ILSWISKGDI ELLPDATVVP DKQPEATHVV

QYGETLSSIA

YQYGTDYQTL AALNGLANPN LIYPGQVLKV NGSATSNVYT VKYGDNLSSI

AAKLGTTYQA

LAALNGLANP NLIYPrCQTLN

Y

EF083-3 (SEQ ID NO:]1S) AAAAG GAGACCAAGG

TGTGGAITGG

GCGATTTATC

CAGATTGGAG

GCAAGTGCTA

AACATGGACA

AATTCCATCG

GGAGGATATG

-ATCCGCAGAA

CTAAATCATG

GCGTATCCTA

GGTATTCTA

GAT~IAACAG

CCAGCAACAG

GATACCGTCA

TGCTAAAAG

GGTATCTTGTl

CCTGATAAGC

GcTTATCAAI

AATC'ITA=T

ACGC LTAAl'

GC-TTTAGCTC

AAT

AAGGTGAACA

GCTACAATGC

TCCCAACG

TTGCGAAAAC

TI'GCATTAGA

TAAGTTCAGA

TCAAACAGGC

TAAACTATCA

TCGATGGTGT

TTCG~cAATT

GAATTACGGA

ATCCACGCGA

AAGTGAAATI

GAAACAGCTP

cATcGATTAC

AACCAGAAGC

ATGGAAcAGZ

ATCCTGGTCJ

7ACGGCGATAJ

;CATTAAACGC

AGGTCGCTTT

TAGCGGTATT

TAAACGTGCG

AACAATGGAT

TT1'TCAACAT

TGCCGAAAAA

TGGcTATACT

ACAAATCATC

GTCACCATAT

CACATCCGCT

TAGTCCTTAT

AGAAATTGAA

TAATGTAGAT

CAAkAGTGCAA

CAAAGGTGAT

GACTCATGTG

CTATCAAACC

kAGTTFI'GAAP 'ITATCTAGI1 3 AITAGCAAA]

GGCTATGCAC

TATGAAcAAT

CATACCTATA

TACTTTMC

CGAGCGTTGG

GCAGCAA.ATA

CCAATGTATT

AAAGAGT'ITC

CCATrGTATG

TATAI'GCAG

ACAGATACCA

.AAAATACCTA

GCT1'GGGCAA

GAAGTAACTG

A=TAATTAT

GTACAATACC

TTGGCGGCAI

GTCAATGG.Al

ATTGCAGCA;

7CCTAACTTGI

ATGATAAATT

ACACATATAA

T1'GGTATr.A

CACGTATTCA

CTAGTr-TTCC cAGAGACAAT

ACAGCTATAA

CTAACTCTTT

CTTATTTCCC

GTGGTTTAGA

ATAAACCAGA

ATTCTGATGT

cTGGGAAC

GAAGCAGAGT

TGCCAGACGC

GAGAAACATT

TAAATGGATT

CGGCAAcAAG

SAACTI'CGCAC

k. TrATCCAGG

:GCTATTGCC

%.ACGCAAGTGC

CACTTCGGGA

AACGCCTAAA

AGATGGATAT

T'FrGTACGC-T GCcATTTACA

ATGGATTGCT

AAGCATGGAT

TGGTAACGTA

AACGGATACG

TAAAGTTGGC

TATTCCGCAA

A71'GcTI'GAA

AACAGTCGTC

ATCAAGTAT

GGCTAATCCA

TAATGTCTAC

TACTTATCAA

TCAAACATTG

174 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. fczecalis Genes.

EF083-4 (SEQ ID NO:316) KGDQGVDWA IYQGEQGRFG

YAHDKFAIAQ

IGGYNASGIY EQYTYKTQVA

SAIAQGXRAH

S IVALDFEHG ALASVPDGYG GYVS SDAEKA NHVNYQQIIK EFPNSLWIA-A

YPIDGVSPYP

LTGITDSGYT DTNKPETDTP

ATDAGEEIEK

VKGNSYI{VQE VTGSRVLLEG

ILSWISKGDI

YQYGTDYQTL AALNGLANPN

LIYPGQVLKV

LAALNGLANP

NLIYPGQTLN

TYIW'YDTWGN

AN'TETILYGM

LYAYFPSMOG

I PNSDVKVGD

ELLPDATVVP

NGSATSNVYT

MDIAKTTMDY

RRIYQAGYTP

IGIWQFTSAY

TVKVKFNVDA

DKQPEATHVV

-\KYGDNLSSI

FLPRIQTPKN

MYYSYXPFTL

IAGGLDGNVD

WATGEAIPQW

QYGETLSSIA

AAKLGTTYQA

EF084-1 (SEQ ID NO:317) TAGTCAAACG TTTATTTTrT CTATTGAATT

GGAGGAGAAC

AGCGGTATGA

TGGCCACGAT

AAAAATCGCC

GTGTI'GGCAA

AATAATCGGC

CCGCAGAAGA

AGCGCATTTT

CACAATTTGA

CACTTAA.AAG

AAGAAGATCA

GTTGATGCGC

TAT'ITAACCG

GTCACAA.AAT

TACTACGAAA

ATTTATGCAC

CGTGTGTTGT

ACTGGTGATG -GCTATAAACT ACCGAATCAC

CTATTATTTC

TCT=TACAAG

ATGTTAATTT

CAAATGGATA

TGCTGTTTAC

*AGTTTTATTA

ACCAAGAATT

'*CTCTTTCCGA

CAAAATCTT

*CTTTCCTTTG

TGGAACTACT

GAAAAATC'IT

TTGTCACAGC

GGACGCAA.AT

TAGTCAATGC

ACTCGAGGCT

ACAATGTTAC

:GCAGAAATI'G

CAGAATACAC

CCTTAAATCC

TATGAAGAAA

TGCGGCCGCC

AAAATLTATTA

AATCATTTCA

TAACTATGAT

CGGACGCATG

CAT1'AACGAA

AGACGATCAA

ATTAACAACT

AGcCAAAAAA

TGAAGAACCT

AACTGTTTTG

ACATTTTGC

AACTCGCAAC

TGAAGAAGTG

GAAAGACTTI

CGCTGGGATI

TTTATIT1"1 AGCTGCATT9

CTATTTACCI

AGAAAAAATC C

TTTGATGTAA

GAAGCAGGCG

C

ATGACTGGTG

C

TTTATTCCTG

ATCATGAACT

TTCCCTGTTA

TTAGGAGTCA

ATAAT1'GGCG

GGCGGCCGCA

ATGGGGCATA

'TTTATCCTGG

AACCAAAAAG

ATTTCAGGAC

GGTAATCAAC

CCTGCCAAAC

CAGTTCACTG

TCCCTCAA6AG

GCTGGTGAAC

CTCACTGGAC

kATTGAAGAAG :CGTAATTAT G .rT1TGTCGGI TCAAGTATT

A

CGGccGCTG I 3GAAIGGAAA 1rTT1TGAATC

CAGATAAATC

CTGTITTAC

TTGAAACCGA

CTTATCCTTC

CCATCAGCCC

ATAAAACGTT

GAAAACCT

CTGCCGCGCT

CTGTCACGGT

AACTAACAGA

TTACGAAAAC

AAGTGACCCC

TTr'TAGATAT

ATGTTGCTGG

TCTAA

;GTACACTAC

'GCTGGGACG

TI GATTGAA

AATGTAACC

=TrT'IATAC

:AATGGTATT

3AAGTCAATT

A.AAAACACAG

ACTGGAAAAA

CACAGGAGCA

GCTCTACCCT

GCAAGGTCTC

AGTTAATCAT

CCGCTGTT-CT

AGCCTTGGAT

AAAGCAACGN

ATTGCCTT

TAAAACAATG

TAATGGCTAT

CTCCCATGCC

EF084-2 (SEQ ID NO:31 8 a MXKF DVIIVGAGTS GMMATIAAAE

AGAQVLLIEK

NRRVGKKLLM TGGGRCNVTN NRPAEEIISF

IPGNGKFLYS

LKEEDHGRHF PVTDKSKSIV DALFNRINEL

GVTVFTKTQV

YAPCVVL ITG GRTYPSTGAT GDGYKLAKKM

GHTISPILYPT

LQDVNLTVLN QKCKPLVNHQ MDMLFTHFGI

SGPAALRCSS

FPTKSFEEVP AKQLTEKQRL SFVELLKDFQ

FTVTKTLPLE

SKLVNGLFFA GELLDINGYT GGYNVTAAFV

TGHVAGSHAA

AFSQFDNYDI

TKLLRKDDQI

ESPIISEEPF

FINQELTRNG

KSFv'rGGGIs EIAEYTYLP I

MNFFESNGIH

IGVETELEKI

ILDKTLQGLS

NQPVTVALDV

LKEVTPKTME

EEV

EF084-3 (SEQ ID NO:319) C GAAGcAGGCG CTCAAGTATT

ATTGAIGAA

AAAAATCGCC GTGTTGGGAA AAAATTATTA

ATCACTGGTG,

AATAATC-GGC CCGCAGAAGA AATCA TTCA ?flTA~CCTG AGCGCATTTT CACAATITTGA TAACTATGAT

ATCATGAACT

CACTTAAAAG AAGAAGATCA CGGACGCATG

TTCCCTCTTA

G MATGCGC TATTTAACCG CATrAACGAA

TTAGGAGTCA

GCGGCCGCTG;

GGAATGGAAA

TITTGAATC

CAGATAAATC

CTGTITTTrAC

TAATGTAACC

ATTTT'rATAC

CAATGGTATT

GAAGTcAATT

AAAAACACAG

TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

GTCACAAAAT

XITTATGCAC

ACTGGTGATG

ACCGAATCAC

TCTTTACAAG

CAAATGGATA

AGT=TTAA

GTGTTTCCGA

CTCTTTG

GAAAAATCTT

GAGAGCAAAT

ACTGGAGGCT

GCAGAAATTG

TACTACGAAA

CGTGTGTTGT

GCTATAAACT

CTATTA=TC

ATGTTAATT'I

TGCTGI'TAC

ACCAAGAATI'

cAAAATCTTT

TGGAACTACT

TTGTCACAGG

TAGTCAATCG

ACAATGTTAC

CAGAATACAC

AGACGATCAA ATAAT1'GGCG

ATTAACAACT

AGCCAAAAAA

TGAAGAAC-CT

AAC~rTTTTG ACAITr-TGGC

AACTCGCAAC

TGAAGAAGTC

GAAAGACTI'

CGGTGGGATT

GGCGGCCGCA

ATGGGGCATA

TTrTATCCTIGG AkACCAAAAAG

ATTTCAGGAC

GGTAATCAAC

CCTGCCAAAC

CAGTTCACTG

TCCCTCAA.AG

TTGC~AACCGA

CTTATCCTTC

CCATCACCCC

ATAAAACGTI'

GAAAACCTTTI

CTGCCGCGCT

CTGTCACCGT

AACTAACAGA

TTACGAAAAC

AAGTGACCCC

TTTTAGATAT

ATGTTGCTGG

TC

ACTGGAAAAA

CACAGGAGCA

GCTCTACCCT

GCALAGGTCTC

AGTTAATCAT

CCGCTGTTCT

AGCCTTGGAT

AAAGCAACGN

ATTGCCTTTG

TAA.AACAATG

TAATGGCTAT

CTCCCATGCC

TTATrTTTT GCTGGTGAAC AGCTGCATT GTCACTGGAC CTATTTACCA ATTGAAGAAG EF084-4 (SEQ ID NO:320) E AGAQVLLIEK NRRVGKKLLM TGGGRCNVTN LKEEDHGRMF PVTDKSKS IV YAPCVVLTTG GRTYPSTGAT LQDVNLTVLN QKGKPLVNHQ FPTKSFEEVP AKQLTEKQR.

SKLVNGLFFA GELLDINGYT NRPAEEI 1SF DALFNR INEL

GDGYKLAKM

MDMLFTHFGI

SFVELLKDFQ

GGYi\=AAFV

IPGNGKFLYS

GVTVFTKTQV

GHTISPLYPT

SGPAALRCSS

FTVTKTIJPLE

TGHVAGSHAA

AFSQFDNY-DI

TKLLRKDDQI

ESPIISEEPF

F INQELTRNG KSFVTGrCGIS

EIAEYTYLPI

MNFFESNG IH

IGVETELEKI

ILDKTLQGLS

NQPVTVALDV

LKEVTPKTME

EEV

EF085-1 (SEQ ID NO:321)

S.

S S S S

S

S

S* S

S

55

TAACCCATGA

CAGTCCACAC

CCGCGGTGTA

AAGGTTT T

TTTGCACACC

TATCAGCCAT

GGGCTGTTTC

GTACTCTATG

GTGGTACATA

CTTTATCAAG

CITFIATCCG

AGTGCGAATA

CTTGGCGCAC

AGGCTGGAG

GCGGCAGCCT

ATGCTTGAAA

ATGATGGACA

G-GGAGAATCG

CCCGA'IC

GGACAAATTG

AATGCGGTTC

GAAAAATACC

AAGCTC'ITC;

CGGTTlrcGGC

GGAAAACACC

AATA=TAA

AATcATTTTG

TCATATCAA-A

GG'ITACCGAT

CCTATACGAT

AATTTGTGTC

TGGTGGAACT

ACTACAATAA

CCACACCGAA

GAGATGATAA

GGCTGACAAT

CGTATATCTT

AAATGGCAAA

TGGCTCACGA

ATGAAATCGC

CTCATGAGTT

*ATATCGGTGA

*GGCAGCGCAA

TACCCATAGC

AAACCTTGGC

ITCCTGTCCGA

AGAACACGCC

GTCTTTCCGI

STCCCAT'TCTA

CTTCCCATCG

TCAGATGGCC

TCCCGCATAT

AATCTGCGGG

TGGAGGAATC

CATTGTCCTC

'TATTTCAGA

GATTCAGAAT

CCAATCCTTI'

TGCCGATACA

TATI'TCGAT]T

TCGGGGA.ATT

TCCGGCAA

CCTGAAAGAA

CATGCATC

AAGGGA.ACAT

AAAAACGCCC

CTACAAAGAC

AACCATCC

CGAACCGCTG

AGAGGCAAAC

TCCGAAGCTC

CCAGGGAGGI

TrTGAACATC

TCGCATTGA

TACAAAAAAC

~TrTGTTCT( GGCGATATGA CTFTGACGGT GATGGCAGCA CGAACTGCCG GAAAATATCA

TCAAAACCAT

ATI'ATAATGG AAAGAAAAGG

GATTTI'CATT

TTACTGCTTG TCGGTGTAAC GGCAACACTG GCGATGGAAG CACAGCAAAC

AGTAAAATCC

AGCGATAGGC TM'ATATGCA

AGAGGTGGCA

GAGTTrTATA TTGAAGATAA

AGAGGGAAGC

TCAAATAGT'G TTAGGCCCGA CTTTC TrAT G ITGCTCAAA GCAAGGCAGC TGTCGGATTG GTTATGATTG- CGATAATGT

TCTATTCAGC

ATGACAACGC CGATCAAAGC

CTTAGCGGAC

GTACCCCGC CGCTGGAGCG AAAGGATGAG ATGTATATCA G-GCTGAAAGA

AACCATCGCA

GAGT=GAGG AAA'CACAGCG

ATATI'TCTIT

ATCGCGGCTG TAAGCGTTCT

GTTGGAGGGA

CATTCTAAGT ATCTGCGCGA

ATGCATCA

GAAATAC TOG AGCTTGTCAG

CCTGAACGAT

GACATAGGGC GCACGGTTGC

CGAGCTGCTA

AACCAGCGGT TCGTCACAGA TAT1'CCAGCC ATCCAAAkAGG CGCTATCCAA TGTCATA ITG 7GAGGTACGGA TATGGAGTGA

GCCTGGGGCT

;GGCGTTCACA TIGATIGATAC

TGCACTTTCA

r' CAGGCGCGAA G-CAGCAAAAA GTGGGCGAAG GCTGGATGCC ATGAGCCTCC

AATATGCGCT

3GCTGGATTTA CCGCCCACAT CAACACTATA 176 TABLE 1. Nucleotidc and Amino Acid Seqeuences of E-faecalis Genes.

EF085-2 (SEQ ID NO:322)

MERKGIFIK

VFSYTI IVLL

LFHYNNQSFE

YQGLTIRGIV

GALAHDMHSM

LENIGDYKDH

DFQTLAEANN

KYRLSVLNNG

LLVGVTATLF

FYI EDKEGSV

MIAIMWVFSL

YIRLKETIAR

SKYLREC 1KM

QRFVTDIPAG

VHIIDDTALSK

AQQFVSYFRA

LYATPNADTS

LCAY IFARQM

LEDEIAREIE

MURQGKTISE

QIVTLSDPKLI

LFIPFYRIDQ

tAEAQQTvKsy QPLVELIQNS NSVRPDFLYV

VH-RDDNISIV

Trp IKALADS ANXMANLKEV LEEFTQRYFFA

AASHELXKPI

ILIELVSLNIDC R IVP IAEPLD QKALSNVILN

AVQNTPQGGE

ARSSKKWAKR

FGACHRTKNA

DRLDMQEVAG

AQSKAGVGLL

PPPLERKDEL

AAVSVLLEGM

IGRTVAE-LLP

VRIWSEPGAE

GCHEPP ICAG KHLRWRFVLA GFTAHINTIN

I

EF085-3 (SEQ ID NO:323) GCATTTCGTGTC TITATTTCAGA GCGATGGAAG CACAGCAAAC

AGTAAAAT

cc

TATCAGCCAT

GGGCTGTTTC

GTACTCTATG

GTGGTACATA

C'ITTATCAAG

CT=TATGCG

AGTGCGAATA

CTTGCCCAC*

AGGCTGGAGG

GCGGCAGCCT

ATGCTTGAAA

ATGATGGACA

GGGAGAATCG

CCCGATTTC

GGACAAATG

AATGCGGTTC

GAAAAATACC

PAr.CTCTTCA

CGGTTTGGGG

GGAAAACACC

AATAT

rGGTGGAACT C ACTACAATAA C

CCACACCGAA

GAGATGATAA

GGCTGACAAT

CGTATATC~r

AAATGGCAAA

TGGCTCACGA

ATGAAATCGC

CTCATGAGT

ATATCGGTGA

GGCAGGGCAA

TACCCATAGC

AAACCTTGGC

TCCTGTCCGA

AGAACACGCC

GTCTTTCCGT

TCCCATTCTA

CTTGCCATCG

TCAGATGCC

;A'rCAGAAT

:CAATCCTTT

E'GCCGATACA

L'ATTTCGATT

rCGGGGAATT

I'GCGCGGCAA

CCTGAAAGAA

CATGCATTCC

AAGGGAACAT

AAAAACCCCC

CTACAAAGAC

AACCATrI'CC

CGAACCGCTG

AGAGCCAAAC

TCCGAAGCTG

CCACGGAGGT

TTITGAACATG

TCGCATTGAT

TACAAAAAAC

'rTrTTCTG AGCGATAGGC ?I'GATATCCA GAGT'FTTATA

TTGAA.GATA-A

TCAAATAGTC;

TTAGGCCCGA

GTTGCTCAAA

GCAAGGCAGG

GTTATGAT1G CGAtAATGGT ATCACAACGC

CGATCAAAGC

GTACCGCCGC

CGCTGGAGCG

ATGTATATCA

GGCTGAAAGA

GAGTTGGAGG

AA.ACACAGCG

ATCGCGGCTG

TAAGCGTTCT

CATCTA-AGT

ATCTCCGA

GAAATACTGG,

AGCTTGT'CAG

CGACATAGGGC'

GCACGGTTGC

AACCAGCGGT

TCGTCACAGA

ATCCAAAAGG

CGCTATCCAA

GAGGTACGCGA

TATGGAGTGA

GGCGTTCACA

TTGATCATAC

CAGGCGCGAA GCAGCAAAA GCTGGATGCC

ATGAGCCTCC

GCTGGATTrA

CCGCCCACAT

GAGGTGGCA

krAGGGAAGC

:ITTCTTTAT

TGTGGGA'rTG

I'GTATTCAGC

CTTAGCGGAC

AAAGGAGAG

AACCATCGCA

ATATTTCTT

GTTGGAGGGA

ATGCATCAAA

CCTGAACGAT

CGAdCTGCTA

TATTCCAGCC

TGTCATAITG

GCCTGGGGCT

TGCACTTTCA

GTGGGCGAAG

AATATGCGCT

CAACACTATA

a a.

a.

a a EF085-4 (SEQ ID NO:324) a a, QFVSYFRA ME.AQQTViKSY QPLVELIQNS

DRLDMQEVAG

LFHYNNQSFE FYIEDKEGSV LYATPNADTS

NSVRPD)FLYV

YQGLTIRGIV MIAIMVVFSL LCAYIFA-RQM TrPIKALADS GALAHDMHSM YIRLKETIAR LEDEIAREHE

LEETQRYFFA

LENIGDYKDH SKYLRECIKM MDRQGKTISE

ILELVSLNJG

DFQTLAEANN QRFVTDIPAG QIVLSDPKLI

QKALSNTVILN

KYRLSVLNMG VHIDCTALSK LFIPFYRIDQ

ARSSKKWAKR

KHLRWRFVLA GFTAHINTIN

I

VHRDDNIS IV AQSKAGVGLL ANKMANKEV

PPPLERKDEL

AASHELKTP I AAVSVLLEGM RIVP IAEPLD

IGRTVAEL.LP

AVQNTPQGGE

VRIWSEPGAE

FGACHRTYKNA

GCHEPPICAG

EF086-1 (SEQ ID NO:325)

TAACTGGTGG

CATGATGACC

CTGTATGCAA

ACAGAT=-

GATTGGCAAA TIGGTCCGC GCAGCGCTAA CAGATACATT

GATTTIATA

TATGAATAC AGATGCAGAA AAA'ITAAATA AATTTACTGC

TCCGCTGATG

AAGATCCAAA CATACAATCG CCAATTTATC GTG-CAACAGG

AGCTA.ACTTA

CAATCACCGT TTTAGGTACT GGACTT'F11GT TAGAAGATAA

TCAACCCCTA

I i I I TABLE 1. Nucleotide and Amino Acid Seqeuences of E faecalis Genes.

GTACAAGTAC

TATCCTGATG

GAGTTGCTAA

AATGACCCTA

GTAAATGGAA

AATCCTTTTA

GCAAAATTTG

CAACAAAGTG

GACTTGAAAA

AATGTATATG

AGTATGTAT

TGGCATACAG

GGATACTGGG

TTGGCAAATG

AATGGACAGG

GTGCTAAAA

ACTGGTACGA

GAAGTGAAGC

ANTCCATTGA

ATAGAAGA.AC

TATACAAATA

TACGAATAIT

AAAGGCTATA

GTCATGATGA

ATGTCGGTTA

TTACAAAATA

GCGGACCCAG

GGCAGCTCGC

AAATTAATTC

GTCTACAGCG

GCAGAAGTAG

CCAACTAAAG

CAAGAAAAAG

*CAAACTCTGG

CTACGTTCGG

AAAAATGGGG

AAACAAGTTA

ACAGGCAAA

TGTCrTTAG

PLAGAAGCTGT

GTTCCTTGAT

AAGGGTTTCG

1ACATT1AGTAA

AAATGCCATC

CTACAGAGTT

CACCAGAAAA

GGTCATACTA

ATGTAGTGAA

GTI'CGATGGA

CACAACGTGT

CAGACGGCAT

CAACGATCGA

GTGCTTATAC

TTCCCTCTAT

AATCTTGGTT

CAGATGCTTC

TTAACCAAGG

ATAACATI'GG

GCTCTGGTCG

CATTTGCTAA

TAACAGTCGT

CTGITCTAGA

ATACATGGAA

FI'CAGAAAA

ATGCATCCGT

AAATT"TCTGT

GTTCAATCAT

AGGAACAAAA

AAGCA PTGAA

ACCAAGCAGA

AAGTAGATAA

ACTACACCGC

CAGATCAAAC

CAGTGAAC

GGAATAATG

AGCCATCAAG

AGAAATCGAT

TTTrTCGTAA TCCGTCCGTT TTAAAAAGTG TCAACATGGT TAT'PTTCCGT ACGAATTCAG ACTATITAC TTTATI'TAAT GTTGTG-GATA GATGGTTTCT GCTAGAAGTA TGAATCGGGT AAAGAAACAA 'ITTAAGAAAT GACAT ITATA TCATTTCTTT AAA.AAACCAA TAGTGCCTCA CCTGCCCAAG TCGAGTCCTA CAGA-AAAATA CGGAACTATC-AAT2Y=GA GCTTTAT'ITA TACAATCAAG TCCATATCGA TTACCAGGAA AGGGAAACGC AGTCCCCAGT AGGAATGTTT TTAGATAAAA C'FrATTAGAT GGTCAAATCA GATTGAAACA ATCCTCGATA TTCAGACAAA GATAATTC'TT CTATGTTrTT CCTAATTCNA CTACGGAGAT ATTAACGAAT AkATTAGTAAA AATTATGGCA TGGGAAAACG AATGA-AGAAA AAATACAGCA AACTTACAAG TAATGACCAA GAAA=TCAG AATTGATAAC GGTGTTTATC T1'CTATTGAA TTTGATAAGG TGACCAAAAT ATTATCACTI' TGTTAAAACA ACTCCCAG AGAACACCAA GAAAAAGACT ACAAGCACAA ACTGTGGCAG AACAGAG [TA CGTTCGGCAG AACCAACTTG TITGAAAATCA AAGCAGTTGG AAAGTCTACA AACAGCAACG CAAGCAGAAG AITAACATTG AAAAATAGTG ACACI'AAAT ACTAGTACAG CCAAGCTIGT 'ITCAGAAAAG CGCGCTTGTG AIATTGGTC AAGTAAATCG AAGAAGTAA

TTCCTCTGG

ACA.ACGGCAG

AAGGTTCCGA

AAGGT'IACTT

T'T TCCAGAGC

TAGCTAAFT

CATCTATCCA

GAGA7=~A

CGACACCAAT

ACGAATATGC

ATACCGAAAA

ACITGCTCA

CGACAGTTGA

CATGCTAGC

GTAATGAAGG

TTAAbTTTGGG

ATCGGATGAT

GGATTAGTTT

TGAATACGCT

ACTITGTTAA

AGACTCGFTGA

TCGCAGCTCT

CCATTGAAGC

G-ACTGTATGC

GCTMhACTCT

GCATTCTI'GA

TAAkATACGC

TAACGAAAGA

ACACCGCA.AG

ATCAAACA.AC

TGAAGCAATI'

TCAAAGAAAA

GTG.AAGCATTr

TAGACCAAGC

GGGAAAATAA

GAGTTGATCA

CTACCCAATI'

'PTCTAGTTAT

TGATCGCT-TA

TTACGGGAAT

CTGGGAGATG

ACAATTGATG

CCCAGAAACG

AACCTTAATT

AACGTGGCTT

AGCGTTAXI"I

GCAATCTITA

GGTGGGGATC

TAAAAAAGC

GTTTGATGAA

CAC-AAGAGAA

TGG-CTCAAAT

AATGAACTTA

AAG-TGGCATT

TCATCtACAG

AAGCGCAGCG

TGATGTTCAA

TGATAA-AACC

AAATGGTACT

TTCTAAAAAC

AGGTAATI'AT

GTATCATCCA

TGCGAATCCT

AGTAGTCGCA

GGGGTTAAAT

AGCC ITAGAA

CAGCTGGAAA

AGCAACGCAA

GGTAAAAGTG

CGAGAAACAC

GAAGCAAGCG

AGAAGCAAAA

AAAGGAGCAA

AACTGGTACG

TTTACCGAGC

CGCCAGTGGG

EF086-2 JSEQ ID NO:326)

LVGI.ANWFRA

DIS ITVLCTG

LLKGFGRIQT

PFTTEFESGK

LKNVVNSASP

HTADGMLYLY

GQVAS IGMFL

VKLNQGSDKD

TNTFAKISKN

lOrINNDQE

DPEISVDQNI

YSEALKQAQT

ALTDTLILU4

LLLEDNQRLV

ILQGSDWEN

ETIANLTLIA

AQATPMQSLN

NQDFAQFDEG

DKSNEGMNLV

NSWISLSAAX

YGKTVENGTY

IAGLYAYDPM

ITLNSAGLNG

VADQTTATQA

DDLLNTDAEK

QVQE-AVPSVL

DPNISNELFNV

KFAPENLRND

VYGSMDRVLQ

YWATIDPYRL

AKKSWFLLDG

PLNNIGYVFP

EYLTVVGKTN

SVISEKI DNG

SSRSIIVKTT

EVDQAETE-LR

LNKPTAPLML

KSVSSGDGLY

VDKGYLQLMV

IYTS IQTWLQ KNNEYAVGI S

PGTIVDTREL

QI INLGSGIT NSMNTLIDVQ I

EEIAALSKNK

VYRLTLANPL

PEVTKEALEK

SAVKQLVKVP

YAKDPNIQWP

POGSLIQHGY

NGKMPSMVSG

QSGSYYHFFK

M4YSQRVGNYE

AN'GAYTGKRS

GTrDAS IETI

EERSGRYGDI

GY'NENTAN.

QNNASVSIEF

L IQEQKEIHQE

?KXVDKTNLL

IYRATGANLT

FPYNGSYGNE

RS ISRAPETN

KPRDFEALID

FGNTENKXGW

PQSWVGGSN

LDNRMI HPQE

NEYFVNDKTY

LQAIE-AGNYV

DKGILEVVAA

KDYTASSWKV

KIIKENEKHQ

178 TABLE 1. Nucleotide and Amino Acid Sequences of E. faecalis Genes.

EKDYTASSWK VYSE-ALKQAQ TVADQTTATQ AEVIDQAEAKL RSAVKRL'TLK

NSGENKKEQK

NGGNNGHLNT STGVDQTCTK QVKPSSQGGF RKASQFLPST GEKXSIALVI

IGLLDVIASGC

LLVFRKSI(SK

K

EF086-3 (SEQ ID NO:327) ACCACAAAA TTTAAGAAAT GACATTTATA CATCTATCCA

AACGTGGCITT

CAACAA.AGTG GGTCATACTA TCATI'CTTT AAA.AAACCAA

GAGATTTTG.

GACTTGAAAA ATCTAGTGAA TAGTGCGTCA CCTGCCCAAC

CGACACCAA

AATGTATATG GTTCGATGGA TCGAGTCCTA CAGAAAAATA

ACGAATATG

AGTATGTAT CACAACGTGT CGGAAACTAT- GAATTTGGGA

ATACGGAAA

TGGCATACAG CAGACGGCAT GCTTTATTTA tACAATCA.AG

ACTITTGCTC

GGATACTGGG CALACGATCGA TCCATATCGA TTACCAGGA

CGACAGTTG

TTGGCAPLATG GTGCTTATAC AGCGA.AACGC AGTCCCCAGT

CATGSGGTAG

AAT

A

C

A

A

A

AGCCGTTAATIT

GcAATCTTTA

GGTGGGGATC

TAAAAAAGGC

GTTTGATGAA

CACAAGAGAA

TGGCTCAAAT

EF086-4 (SEQ ID NO:328) PENLRND IYTSIQTWLQ QSGSYYHFFK

KPRDFEALID

LKNVVNSASP AQATPMQSLN VYGSMDRVLQ KNNEYAVGIS

MYSQRVGNYE-

HTADGMLYLY NQDFAQFDEG YWATIDPYRL PGTIVDTREL

ANGAYTGKRS

FGNTENK(KGW

PQSWVGGSNN

EF087-1 (SEQ ID NO:329) TAACTGGTGC GATTGGCAAA

TI

CATGATGACC TATTGAATAC

AC

CTGTATGCAA AAGATCCAA-A Cl ACAGATATIT CAATCACCGT

T

GTACAAGTAC A.AGAAGCTGT

T(

TATCCTGATG GTTCCTTGAT Ti GAGTTGCTAA AAGGGTTTCG

A

AATGACCCTA ACATTAGTAA

T

GTAAATGGAA AAATGCCATC

G

AATCCTTTTA CTACAGAGTT

T

GCAAAAT'ITG CACCAGAAAA CAACAALAGTG GGTCATACTA

TI

GACTTGAAA-A ATGTAGTGAA AATGTATATrG

GTTCGATGGA

AGTATGTATT CACAACGTGT

C

TGGCATACAG

CAGACGGCAT

GGATACTGGG

CAACGATCGA

TTGGCAAATG

GTGCTTATAC

AATG;GACAGG

TTGCCTCTAT

GTTIGCTAAAA

AATCTGGT'

ACTGG.TACGA

CAGATGCTTC

GAAGTGAAGC

TTAACCAAGG

ANTCCATTGA

ATAACMTTGG

ATAGAAGAAC

GCTCTGGTCG

TATACAAATA

CATTTGCTAA

TACGAATATT TAACAGTrGT AAAGGCTATA cTGTTCTAGA GTCATGATGA

ATACATGGAA

ATGTCGGTTA ?I2TCAGAAALA TTACAAAATA

ATGCATCCGT

ATGCAGAA A kTACAATGG C' rTAGGTAcT

G

CCGTCCG T

CAACATGGTT

CGAA'rrCAG A TTATT1AAT

G

ATCGTTTCT

G

GAATCGGGT

TTAAGAAAT C 'CATTTCT?1T

!AGTGCGTCAC

.CGAGTCCTA

:GGAAACTAT

;cTTTATI'TA rcCATATCGA

AGGGAAACC

AGGAATG'Tr cTTATTAGAT

GATTGAAACA

TTCAGACAAA

CTATGTTT

CTACGGAGAT

AATTAGTAAA

TC.GGAAAACG

AAATACAGCA

TAATGACCAA

AATTGATAAC

TTCTAT'rGAA kAITAAATA A.; CAATTTATC G~ GACT'T

TI

TAAA.AAGTG T AT'ITTCCGT A( CT WITTTAC A TTCGTGGATA

A

~GTAGAAGTAT

AAGAA.ACAAT

;ACATTTATA C ~LAAAACCAA C :CTGCCCAAG

C

:AGAAAAATA

P

GAA=rGGGA TACAATCAAG

I

TTACCAGGAAC

AGTCCCCAGT

TTAGATAAAA

GGTCA.AATCA

ATCCTCGATA

GATAALTTC~r ccTAATTCNA A'rrAACGAAT

A.AITATGGCA

AATGAAGAAA

AACTTACAAC

GAAATIGMCAG

GGTGT'P1ATC

TTTGATAAGG

~GGTTCCGC GCAGCGCTAA %GATACAT GP TTTAcTGC TC GCAACAGG AC kGAACATAA TC rrCCTCTGG- T( AACGGCAG T' p1rCrTTcCA C ACGT-TACTT A TTCCAGACC G AGCTAATTT

A

ATCTATCCAA

,AGATTTTGAA

GACACCAAT

G

~CGAATATGC G TACGGAAAA qj ~CTTIICTCA C MACAGTTGAc

CATGGGTAGG

GTAATGAAGG

TTAATTGG

PTCGGATGAT

GGATTAGTTT

TGAATACGCT

ACTTTGTTAA

AGACTGTI'GA

TCGCAGCTCT

CCATTGAAGC

GACTGTAT~c

GCTTAACTCT

GCATTCTI'GA

~TTTATTA

.CGCTGATG

,CTAACTTA

.AACGCCTA

;ATGGCT]TA

rACGGGAAT

TGGGAGATG

CAATTGATG

CcAGAAACG

ACCTTAA'T

ACGTGGCTT

,CCGTTAAT]T

:CAATCTTTA

,GTGGGGATC

'AAAA.AAGGC

TMATGAA

.ACAAGAGAA

rGGCTCAAAT AATGAACT1'A

AAGTGGCATT

TCATCCACAG

AACCGcAGCG

TGATGTTCAA

TGATAAAACC

AAATGGTACT

TTCTAAAAAC

AGGTAATTAT

G;TATGATCCA

TGCGAATCCT

AGTAGTCGCA

179 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

GCGGACCcAG

GGCAGCTCC

AA-ATTAATTC

GTCTACAGCG

GCAGAAGTAG

CCAACTAAAC

cAAGAAAAAG

CAAACTGTGG

CTACGTTCGG

AAAAATGGGG

A).AcAAGTTA

ACAGGAGAAA

TGTCTTTAG

AAATTTCTGT

GTTCAATCAT

AGGAACAAAA

AAGCATTGAA

ACcAAGCAGA kACTAGATAA

ACTACACCGC

CAGATCAAAc cAGTGAAGCG

GGAATAATGG

AGCCATCAAG

AGAA.ATCGAT

TTTTI'CGTAA

TGACC-AAAAT

TGTTAAAACA

AGAACACCAA

ACAAGCACA

AAcAGAGTTA AAccAAC'1r AAGcAGTTGG AAcAGCAACG

ATTAACATI'G

ACAc?1TAAAT

CCAAGGTGGT

CGCGcTTGTG

AAGTAAATCG

ATTATCACTT

ACTCcTGAAG

GAAAAAGACT

ACTGTGGCAG

CGTTCGGCAG

TTGAAAATCA

AAAGTCTACA

CAAGCAGAAG

AAA.AATAGTG

ACTAGTACAG

TTqAGAAAAG

ATTATTGGTC

AAGAAG'TAA

TAkATACTGC

TAACGAAAGA

ACACcGcAAG

ATCAAACAAC

TcAAGCAATT

TCAAAGAAAA

GTGAAGCATT

TAGACcAAGC

GGGAAAATAA

GAGTTGATCA

CTACGCCAATT

TTC:TAGTTAT

cGGGTTAAAT

AGCGTTAGAA

CAGCTGGAAA

ACAACGCA

GGTAA.AAGTrG

CGAGAAACAC

GAACCAAGCG

AGAAGCAAAA

APLAGGAGCAA

AACTGGTACG

'rrTACCGAGC

CGCCAGTGGG

EF0872 (SEQ ID NO:.330)

INGLANWFRA

DIS ITVLGTG

LLKGFGRIQT

PFTTEFESGK

LJKNVVNSASP

HTADGMLYLY

GQVAS IGMFL

VKILNQGSDKD

TNTFAK ISKN

MMNTWNNDQE

DPEISVDQNI

YSEALKQAQT

EKDYTASSWK

NGGNNGHLNT

ALTDTLILLH

DDLLNTDAEI(

LLLEONQRLV

ILQGSDWEMN

ETIANLTLIA

AQATPMQSLN

NQDFAQFDEG

DKSNEGb4NLV

NSWISLSAAX

YG1(IVENG IY

IAGLYAYDPM

ITLN~SAGLNG

VADQTTATQA

vysEAIJKQAQ

STGVDQTGT<

QvQEAVPSVL

DPNISNLFNV

KFAPENLRND

VYGSMDRVLQ

YWATIDPYRL

AKKSWFLLDG

PLNNIGYVFP

7EYL IVVGKTN'

SVISEKIDNG

SSRSI IVKITT

EVDQAETELR

TVAE)QTTATC

QVYPSSQGGE

LtqKFT-APLMli

KSVSSGDGLY

VDKGYLQLKV

IYTSIQTJJLQ

KNNEYAVGIS

PG ITVDTREL QI INLGSGIT NSMN2TLDVQ

I

EEIAALS(NK

VYRLTLANPL

*PEvTKF.ALEI< *SAVKQLVKVrP

AEVDQAEAKI

*RKASQFLPS3

YAKDPNIQWP

PDGSLIQHGY

NGKMPSMVSG

QSGSYYHFFK

MYSQRVGNY-E

ANGAvYTGKRS GTTD ASIETI

EERSGRYGOI

GYTVLMNTAN

QNNASVS IEF

LIQEQKEHQE

TYEVDKTNLL

RSAVKRLTLK

GEKXSIALVI

IyRATGANLT

FPYNGSYGNE

RS ISRAPETN

KPRDFEALID

FGNTENKKGW

PQSWVJGGSNN

LDNRMIHPQE

NEYFVNTJKTY

LQAIEAGNYV~

DKG-ILEVVAA

KDYTASSWKV

KI IKENEKHQ

NSGENKKEQK

IGLLVIASGC

a LLJVFRKSKSK

K

EFOB7-3 (SEQ ID NO:331) A ATCGGATGAT

TCATCCACAG

GAAGTGAAGC

ANTccAI'GA

ATAGAAGAAC

TATAcAAATA

TACGAATATI'

AAAGGCTATA

GTCATGATGA

ATGTCGGTTA

TrAAcCAAGG

ATAACATTGG

rGcTCTGGTCG

CAICTAA

TAACAGTGGT

CTGTTCTAGA

ATACATGGAA

TTCAGAAAA

TTCAGACAAA

CTATGTTTT

CTAcGrGAGAT

AMTTAGTAA.A

TGGGAAAACG

AAATACAGCA

TAATGACCAA

AAT'rGATAAC

GATAATTCTT

ccTAATTCNA

A'FTAACGAAT

AA ETATGGCA

AATGAAGAAA

AACTTACAAG

GAAATTGCAG

GGTGTTITATC

GGATTAGI'T AAGCGCAGCG TGAATACGCT TyGATGTTCAA ACTTGTTAA

TGATAAAACC

AGACT,3TTGA

AAATGGTACT

TCGCAGCTCT

TTCTAAAAAC

CCATTG-AAGC

AGGTAATTAT

GACTGTATGC

CTATGATCCA

GCTTAACTCT

TGCGAATCCT

TTACAAAATA

ATGCATCC

EF087-4 (SEQ ID NO:332)

NRMIHPQE

VKLNQGSDKD NSWISLSAAX PLNNIGYVFP NSMNTLDVQI EERSGRYGDI

NEYFVNDYTY

TNTFAKI SKN YGKTVENGTY EYLTNVGKTN EEIAALSKN3( GYTVLENTAN

LQAIEAGNYV

MMNTWNNDQE IAGLYAYDPM SVISEKIDNG VYRLTLANPL

QNNAS

EF088-1 (SEQ ID NO:333) TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

TAACTGGTGGC

CATGATGACC

CTGTATGCAA

ACAGATA'IT

GTACAAGTAC

TATCCTGATG

GAGTTGCTAA

AATCACCCTA

GTAAATGGAA

AATCCTTIA

GCAAAATTTG

CAACAAAGTG

GACTTGAAAA

AATGTATATG

AGTATGTATT

TGGCATACAG

GGATACTGCG

TTGGCAAATG

AATGGACAGG

GTTCCTAAAA

ACTGGTACGA

GAAGTGAAGC

ANTCCATTGA

XTAGAAGAAC

TATACAAATA

TACGAATTT

AAAGGCTATA

GTCATGATGA

ATGTCGGTI'A

1'rACAAAATA

GCGGACCCAG

GGCAGCTCGC

AAATTAATTC

GTCTACAGCG

GCAGAAGTAG

CCAACTAAAG

CAAGAAAA-AG

CAAACTGTGG

CTACGTTCGG

AAAAATGCGGG

AAACAAGTTA

ACAGGAGAAA

TGTCTTITAG

~A'TGGCAAA TI L'ATTGAATAC k.AGATCCAAA C :AATCACCGT k~AGA.AGCTGT I1 GTTCCT1'CAT ~I ~AGGTTTGG

ACATTAGTAA

AAATGCCATCC

CTACAGAGT

CACCAGAAAA

GGTCATACTA

PTGTAGTGAA

GTTCGATGGA

CACAACGTGT

CAGACGGCAT

CAACGATCGA

GTGCTTATAC

TTGCCTCTAT

AATCTIGGTT-

CAGATGCTTC

TTAACCAAGG

ATAACATTGG

GCTCTGGTCG

CATTI'GCTAA

TAACAGTGGT

CTGTTCTAGA

ATACATGGAA

TTTCAGAAAA

ATGCATCCGT

AAA'TrTCTGT

GTCAATCAT

AGGAACAAAA

AAGCATIGAA

ACCAAGCAGA

AAGTAGATAA

ACTACACCGC

CAGATCAAAC

CAGTGAAGCG

GGAATAATG

AGCCATCAAG

AGAAATCGAT

TTTTCGTAA

'TGGTTCCC

,GATGCAGAA

:ATACAATGG

~TTAGGTACT

~CCGTCCGTT

~CAACATGGT

CCAATTCAG

.FrATITTAAT ;ATGTrrCT

MGAATCGGCT

ETAAGAAAT

ECA=TC=T

[AGTGCGTCA

ICAGTCCTA

:GGAAACTAT

GCTTTATTTA

TCCATATCGA

AGGGAAACGC

AGGATIGT

CTTATTACAT

GATI'GAAACA

TTCAGACAAA

CTATGTTTT'

CTACGGACAT

AATTAGTAAA

TGGGAAAACG

AA.ATACAGCA

TAATGACCAA

AATTGATAAC

TTCTA'ITGAA

TGACCAAAAT

TGTTAAAACA

AGAACACCAA

ACAAGCACAIA.

AACAGAGTTP

AACCAACTTIC.

AAGCAGTTGC

AACAGCAACC

ATTAACATTC

ACACTTAAA]

CCAAGGTGG'

CGCGCI'GTC

AAGTAAATC(

GCAGCGCTAA

AAATTAAATA

CCAATTTATC

GGACTT=TT

TAAAAAGTG

TATTTTCCGT

ACTATTTTAC

GTTGTGGATA

GGTAGAAGTA

AAAGAAACAA

GCAXFITATA

AAAAAACCAA

CCTGCCCAAG

CAGAAAAATA

GAATTTGGGA

TACAATCAAG

TTACCAGGAA

AGTCCCCAGT

TTAGATAAAA

GTrAAATCA

ATCCTCGATA

GATAAITCIT

CCTAATTCNA

ATTAACGAAT

AAkTTATGGCA

AATGAAGAAA

AACTI'ACAAG

GAAATTGCAG

GGTGTTTATC

TTTGATAAGC

ATTATCACTI

ACTCCTGAAC

GAAAAAACI

ACTGTGGCAC

LCGTCGGCAC

TTGAAAATC)

AAAGTCTAC)

CAAGCAGAA(

AAAAATAGT(

ACTAGTACAC

T TCAGAAAA( ;AlrATTGGT(

;AAGAAGTAA

CAGATACATT

AATTTACTGC

GTGCAACAGG

TAGAAGATAA

'ITTCCTCTGG

ACAACGGCAG

AAGGTTCCGA

AAGG ITACTT

TTTCCAGAGC

TAGCTAAM

CATCTATCCA

GAGATTTTGA

CGACACCAAT

ACGAA'VATGC

ATACGGAAAA

ACTT-TGCTCA

CGACAGTTGA

CATGGGTAGG

GTAATGAAGG

TTAA'-TTGG

ATCGGATGAT

GGATTAGTTT

TGAATACGCT

ACITTrGTTAA *AGACTG ITGA

*TCGCAGCTCT

CCATTGAAC

GACTGTATGC

*GCrAACTCI

*GCATTCI'GP

TAAATAGTGC

TAACGAAAG;

SACACCCCAAC

ATCAAACAAC

TGAAGCAATI

k TCAA-AGAAAJ k. GTGAAGCATj

TAGACCAAGC

C GGAAAATAJ

.GAG=ATCJ

3CTAGCCAAT.

TTCTAGTTA.

GATTTTATTA

TCCGCTGATG

AGCTAACITA

TCAACGCCTA

TGATGGCTTA

TTIACGGGAAT

CTGGGAGATG

ACAATTGATG

GCCAGAALACG

AACCTTAATT

AACGTGGCT

AOCGTTAATT

GCAAT'CTTTA

GGTGGGGATC

TAAAAAAGC

GTFI'GATGAA

CACAAGAGAA

TGGCTCAAAT

AATGAACTTA

AAGTGGCATT

TCATCCACAG

AkAGCGCAGCG

TGATGTTCAA

TGATAAAACC

AAATGGTACT

TTCTAAAAAC

AGGTAATTAT

GTATGATCCA

TGCGAATCCT

AGTAGTCGCA

GGGGTTAAAT

AGCGTI'AGAA

CAGCTGGAAA

AGCAACGCAA

7GGTAAAAGTG-

CGAGAAACAC

r GAAGCAAGCG

,AGAAGCUAAA

k AAAGGAGCAA k AACTCCTACG r 1TTACCGAGC r CGCCAGTGCC- EF088-2 (SEQ ID NO:334)

LVGLANWFRA

DISITVLGTG

LLKGFGRIQT

PFTI'EFESGK

LKNVVNSASP

HTADGMLYLY

GQVAS IGMFL

VKLNQGSDKD

ALTDTLILLH

£LLEDNQRLV

ILQGSDWEMN

ETIANLTLIA

AQATPMQSLN

NQDFAQFDEG

DKSNEGMNLV

NSWISLSiAX

D)DLL.NTDAEK

QVQFEAVPSVL

DPNISNLFNV

KFAPENLRND

VYGSMDRVLQ

YWATIDPYRL

AKXSWFLLDG

PLNNIGY'VFP

LNKFTAPLML

KSVSSGDGLY

VDKGYLQLMV

IYTS IQTWLQ

KNNEYAVGIS

PGTTVDTRZEL

QI INLGSGIT

NSMNTLDVQI

YA1KDPNIQWP

PDGSLIQHGY

NGMPSMVSG

QSGSYYHFFK

MYSQRVGNYE

ANGAYTGKRS

GITDAS IETI

EERSGRYGDI

IyRATGANLT

FPYNGSYGNE

RS ISRAPETN KPRDFEALI D

FGNTENICKGW

pQSWVGrSNN

LDNRMIHPQE

NEYFVNDKTY

TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

TNTFAKI SKN YGKTVENGTY bMMII\TWNNDQE IAGLYAYDPM DPEISVDQNI ITLNSAGLNG YSEALKQAQT VADQTTATQA EKDYTASSWK VYSEALKQAQ NGGNNGHLNT STGVDQTG--'K LLVFRKSKSI( K EYLmVVGKTN

SVISEKICNG

SSRSIIVKTT

EVDQAETELR

TVADQTTATQ

QVKPSSQGGF

EEIAkALSKNK

VYRLTLANPL

PEVTKEAkLEK

SAVKQLVKVP

AEVDQAEAKL

RKASQFLPST

GYTWLETAN

QNNASVS I EF

LIQEQKEHQE

TKEVDKTNLL

RSAVKRLTLK

GEKKS IALVI LQAI EAGNY-V DKG ILEVVAA

KDYTASSWKV

KIIKENEKHQ

NSGENKKEQK

IGLLV IASGC EF088-3 (SEQ ID NO:335) A ACTCCTGAAG TAACGAAAGA AGCGTTAGAA

AAATTAATTC

GTCTACAGCG

GCAGAAGTAG

CCAACTAAAG

CAAGAAAAAG

CAAACTGTGG

CTACGTTCGG

AAAAATGGGG

AAACAAGTTA

AGGAACAAAA

AAGCATTGAA

ACCAAGCAGA

AAGTAGATAA

ACTACACCC

CAGATCAAAC

CACTCAAGCG

GGAATAATGG

AGCCATCAAG

AGAACACCAA

ACAAGCACAA

AACAGAGTTA

AACCAACTTG

AAGCAGTTGG

AACAGCAACG

A'ITAACATTG

ACACTTAAAT

CCAAGGTGGT

GAAAAAGACT

ACTGTG.GCAG

CGTTCGGCAG

TTGAAAATCA

AAACTCTACA

CAAGCAGAAG

AAAAATAGTG

ACTACTACAG

TTCAGA.AAAG

ACAC CGCAAG

ATCAAACAAC

TGALAGCAATT

TCAAAGAAAA

GTGkAGCATT TAGACf-AAGC

GGGA-AAATA.A

GAGTTGATCA

CTAGCCAAPT

CAGCTGGAAA

AGCA.ACGCAA

GGTAA.AAGTG

CGAGAAACAC

GAAGCAkAGCG

AGAAGCAAAA

AAAGGAGCAA

AACTGGTACG

=IACCGAGC

ACAGGAGAAA AGAAA EF088-4 (SEQ ID NO:336) T PEVTKEALEK LIQEQKEHQE KDYTASSWKV

YSEALKQAQT

EKDYTASSW<

NGGNNGHLNT

VADQTTATQA

VYSEAILKQAQ

STGVDQTGTK

EVDQAETELR

TVADQTTATQ

QVKPSSQGGF

SAVKQLVKVP

AEVDQAE.AKL

RKASQFLPST

TKEVDKTNLL

RSAVK-RLTLK

GEKK

KIIKENEKHQ

NSGENKKEQK

S

S

S

S

S

S

**SS

S. S S

S*

EF089-1 (SEQ ID NO:337)

TGACAGATAC

TATAGGTCAA

AATGACAGAC

TGTATGTTAT

ACACCAACAA

GCGCCTGGTG

ACCATTGAAA

CAAAACGGGA

CCGAAAGAAA

CCTAAAGATr

GAAACAACGA

GTTGTGGCTA

G4GGGTTAAAC

CAAGCGGCCT

C'TrACCAAT

ATTTCTTTAA

GGTGTAAAAG

CTGTACAAAT

AATGAAAAAG

ATCA ITCTAG

GAACAACAAT

ACCTGCTAAC

AAA'rTT=TG

ATAGGAGAAT

LTGGCTGGAT

'TCCCGAAAA

CCAAACAAAC

ATACGGTGAA

TCAAACCTGA

TCATCTT(GCC

CTlrTATGG

CTTCTGCGGA

TATTCTTCA

CAGGCCAAGT

ATTTAAACCA

CCGATACTGA

AAGGGGAACG

ATGAA.AAGGG

GGGAATTTAC

ACGTAACCAT

CGCTGCTCT

CTGAGCAATA

ACAGGAAACT

GCTTATCTT

GAATATGAAC

TGGCGTGGAG

TCAAGTGGAT

CGTAGAAATT

CTCAGCGACA

CAAAACCTTA

GA.AGCATTCC

CGTGCATGGCT

TCAATCAAAA

GCAAAATGAG

CAACGCGCGA

Al'rACXITIA

GGATATGCAA

AI'TAACGCCA

CACCTATCAA

AAAAGAATT

TAAAGGAACC

ATTGATTITTC

AAGAACGACA

CGGTCTITG

AGATGGAAAG

GCGCACGCTT

AAATCAAAAA

CAGTTACGCA

ACAAATTTAA

CGTrAACT

CAAAAGACCT

GGCGGTATAA

GGGITA~cTA ACAAAGGTT1C

AACGTCATCA

ATCAACACTG

GTGGCGCcAA

GGAAAATATG

GTCAAAGGCG

ACTATTTC~TG

AATTGGTGGT

TCIGTATC

GCATACACGC

GTGCTATAA

TATATGCAAC

CTGAATIIAA

CCTACTFITGA

ATGATACAGA

AT-GGCGTAGT

TAAAAGATTA

TACCTIAAC

CACTCAAAGA

TTAATAATGA

A.ACCAGAITI

ATGTTrCT=

TTCAAAAGG

ACTCTAAC TT

TC'ITGAAATC

CCAATCGTGA

GGGACGTCGC

TGTATCT"ACT

GTAA.AAAGAA

AAGATCGGGA

TACAACAAAG

GGTAATCGCT

'TITTGCGGTC

CTrAAAAATG

TGAAGACATT

AGAATATGGC

TGTGGAAGCA

CATTACGATG-

GAAAAAGAAA

ATACTCCTAT

AAAATTACTG

ACAAAACCCA

AGGCGAAACG

TAGTTACCCA

AACGGCCTAT

AGAACGGTAC

TAAAGAA'ITA

GATTGCAIA

AAAAGAGGAA

EF089-2 (SEQ ID NO:338) 182 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

MNR WKVYATVIAC MLFGWIGVEA HASEFNFAVT IENTVNSATT NLNGVVEYGQ KDSFDGVMAG GITLKEXKI(E VKPGQVNARN VINVSLQNPQ SLKGERLTPG KYVLKSTAYG EKDVTIKGTN WWLYLLIALI

PTIPENQVDK

NG IKPCKTLR

T'ITSALDQSKG

AAYLNQLHLI

VKDEKGTYQV

ILALLLLIFF

SKTYFDLKMA

FNLKDYVE:AP

LAINNEYSYV

NTVSKGGIETL

KGANGEERYL

LYRKKKKEEE

PGAKQ'IVEIQ

KEIILPKHSQ

VAI ILQQNET

YQSDTEDMQV

YKWEFTK'tEFT

QQSEQ

LRNDTDED IT KTLPLT ITMP

KVQPDLKLLG

APNSNFSYP I

ISGDVAKELN

EF089-3 (SEQ ID NO:339) T CTGAXTAA TTTTGCGGTC ACACCAACAA TrCCCGAAAA GCGCCTGGTG CCAAACAAAC ACCATTGAAA ATACGGTGA.A CAAAACGGGA TCAAACCTGA CCGAAAGAAA TCATCTTGCC CCTAAAGATT CTTTTGATGG GAAACAACGA CTTTGCGGA GTTGTGGCTA WI'ATTCTTCA GGGGTTAAAC CAGGCCAAGT CAAGCGGCCT ATI=TAAACCA CTTTACCAAT. CCGATACTGA A TTT CTTTAA AAGGGGAACG

TCAAGTGGAT

CGTAGAAATT

CTCAGCGACA

CAAAACCTTA

GAAGCAT'CC

CGTGATGGCT

TCAATCAAAA

GCA.AAATGAG

CAACGCGCGA

ATTACATTTA

GGATATGCAA

AT

AAATCAAAAA

CAGTTACGCA

ACAAATTTAA

CGTT'ITAACT

CAAAAGACCT

GGCGGTATAA

GGGTTAGCTA

ACAAAGGTTC

AACGTCATCA

ATCAACACTG

GTGGCCCCAA

CCTACTTTGA

ATGATACAGA

ATGGCGTAGT

TA-AAAGATTA

TACCTTTAAC

CACTCAAAGA

TTAATAATGA

A.ACCAGATTT

ATGT=C=I

TTTCAAAAGG

ACTCTAACTT

C'TAAAAATG

TCAAGACAT

AGAATATGGC

TGTGGAAGCA

CATTACGATG

GAAAAAGAAA

ATACTCCTAT

AAAATTACTG

ACAAAACCCA

AGGCGAAACG

TAGTTACCCA

EF089-4 (SEQ ID NO:340) SEFNFAVT PTIPENQVDK SKTYFDLKiMA PGAKQTVEIQ LRNDTDEDIT IENTVNSATT NLNGVVEYGQ NGIKPDKTLR FNLKDYVEAP KEIILPK4SQ KDSFDGVMAG GITLKEKKKE TTTSADQSKG LAINNEYSYV VAIILQQNET VKPGQVNARN VINVSLQNPQ A.AYLNQLHLI NTVSKGGETL YQSDTEDMQV

SL.KGER

S S

S.

S.

S

S

S

S.

S S

S

55 *5

S

S.*S

5**S

S

S

S S

SS

KTLPLTITMP

KVQPDLKLLG

APNSNFSYPI

EF090-1 (SEQ ID NO:341)

TAGTCTCTAA

GAAATCCAAA

ATTATTACTT

AGTCACTITT

GCTGCAGAAG

ACAAAAGAAG

GACCATAC

GAAGCCAAAT

CGTCGAGGCA

CAGCGCTTAA

AATGCCAAAG

ACATTGACAA

AAAGTAGCCT

AAAGAAAAAC

CCAGTTAT

GGCTGGTCGA

GAACAAACGA

GCCAATAATT

AAAAAAATAG

GAAATAAACC

TCCTAATAC

C=TGGTTGG

TNCCAACCAC

AAAAAI'AC

AAAAAATTCA

ACAGTAGCAA

TAGCAACTTT

ATGGCACTT

ACCAGCAGAT

ATTTTTATAA

CGCTCAATAA

TTGATAAAAC

TAACTACTTG

TTACAGATGT

TTGATGGGGC

ATGCAATCAC

ACATTGAAAT

TCACCACAGA

TAAAATTATT

ACCTCCTCAA

ACTAGCTCTG

AAAAGTAAAT

GCAAGTTAAT

ACTTCCTAAA

GGTGAAGCTA

GAGTTTTCCA

TGAAATTGTE'

TATTGCGGAT

ACCCCCTGAA

CAAGTTAAAT

ACAAAI'CAA

GGTGACACAA

TCACGGCACG

CA.AAACGCAA

TG.CTCGTTG

TGATFI'AAA.A

TGTCATTACT

CATATA.AAGG ATCAACAA.AT GAAAAAAGAA AAATCAGGAA AAAA.TAATAA AAAGAAAGTA GTTGCTGGGG GCAGTTATGT TrA'TrrTcAA GGAC'F11CTG TAGGCTGGTT AAATGTAAAT CAA.ACCGAAG AAGTTGGGT TCAAACCGGGG AAATACCAAT TGGATCAKAA ATTTTTAAAA CCGTTAAACG AGGCATTCAA AAAAGAACTA CAGGCGAAAC CAAGCAAAAA TGCGAGTATC CCCGAAGAAC AAGGCACAGT AGTGGACACA GTTGAAGCGG GAAA-AGGCAA CTATCAATAT ATrACAAAAG AGGATCAAAC GTrAA-AGGCA AAJCAATTA CAGTTGATAT TAATGGTGAA AACGTGCTGA ATGATCATGG CACAATCAAC TTACAAACAA CATATGTC TGCTAATCAA ACACGTCGTI TTAAAAACAA CGGAAGTTAT GAACTACTAG TAAACGCGCT GAATAGCCA GTTGGTGATA CCAAAGAAAA TAGTAAAATT GATCAAAAAA TGTATITGTTT CAITCATGGC GGCAGATATA ACAAAGGAAC CGCAACAGTA 183 TABLE 1. Nucleotide and Amino Acid Seqeuences of Efadecalis Genes.

CCAGGATTCC

GATGG'ITCTC

GTTGTCACAC

GAAGCCTTTA

TCAAAAATCT

GATGATGCAC

ATACAATI

GATACAGTGT

AAATCGGGAT

AAACCGATC

TTGATGTATC

CAGGTGAATT

ATATCGGACA

GCCAGTAAAA

TCATGACTCC

TGGTACTAAT

CTATGACGGA

TGATAAACCT

ACCCATCGTGA

TATTCGATGC

GACCATA-AAT

GGCTGTATCA

ATGCCGGTAA

GTAGATTACG

ATTTAGA.AGG

CGTI'ATTAAG

TGGATAAGTA

ATACCCCAGG

TATTTATGG

GCGAAGAAGT

TCAAATGCTT

TCAAGGGGGC

TGGCGATAAA

AACAGAAGTT

ACATATCTAT

ATAA

EF090-2 (SEQ ID NO:342) MRNTRRQK SGKNNKKKVI ITSLVGLALV AGGSYVYFQS

HFXPTTKVNG

HL.HSSKVKLP

RLNQQ I ADV

VAFDKTQIQN

WS IDCAKTQE

KIVTTDVITG

VTQIGIHDSD

VSVGWLNVNA

LNEAFKKELE

EAGKGNYQYN

VLNODGTINK

LLVNALNSQE

RYNKGTATVP

HKLDKYGDKE

AEEKLAQVNQ

AKLATLSFPE

AKDFYKAPEI

EKLTTWVTQL

QTNAITAPLV

GFHTILYRTT

AFKTD-AGSNG

TEEVVVQT

GKPSKNAS IR

TKEDQTLKAT

ETTYGSANQP

GDTKENTSKIA

DVNLEGQMLD

CINTPGTEVS

KEEKIQLPKK

RGNGTFEIVP

LTTLNNkLNIK

VLFTDVHGTT

NNYIEIDLKO

GSRYSVPVKY

K IFOVSYDGM

YQLDQKFLKD

EEQGTVVDTQ

TITVD INGEK

RRFKNNGSYG

QKMYCFIDGK

WMPLLSQGGV

PVIIYGHIYD

DAPGEFDKPV DYGEEV EF090-3 (SEQ ID NO:343) CAC AAAAGTAAAT GGAGTTTCTG TAGGCTGGT' AAATGTAAAT

GCTGCAGAAG

ACAAAAGAAG

GACCATITAC

GAAGCCAAAT

CGTCGAGGCA

CAGCGCTTAA

AATrGCCAAAG

ACATTGACAA

AAAGTAGCCT

AAAGAAAAAC

CCAGT'FTTAT

GGCTGGTCGA

GAACAAACGA

GCCAATAATT

AAAAAAATAG

CCAGGATTCC

GATIGTTCTC

GTTGTCACAC

GAAGCCTTTA

TCAAAAATCT

GATGATGCAC

AAALAATTAC

AAAAAATTCA

ACAGTAGCAA

TAGCAACTTT

ATGGCACTTT

ACCAGCAGAT

fATAA

CGCTCAATAA

TTGATAAAAC

TAACTACI'G

TTACAGATGT

TTGATGGGGC

ATGCAATCAC

ACATTGAAAT

TCACCACAGA

ATACAATTT

GATACAGTGT

AA.ATCGGGAT

AAACCGATOC

TTGATGTATC

CAGGTGAATT

GCAAGTTAAT

ACTTCCTAAA

GGTGAAGCTA

GAGTTTTCCA

TGAAATTGTT

TATTGCGGAT

ACCCCCTGAA

CAAGTTAAAT

ACAAATTCAA

GGTGACACAA

TCACGGCACG

CAAAACGCAA

TGCTCCCTTG

TGATTTAAAA

TGTCATITACT

ATATCGGACA

GCCAGTAAAA

TCATGACTCC

TGGTAGTAAT

CTATGACGGA

TGATAAACCT

CAAACCGAAG

AAATAC CAAT

CCGTTA.AACG

GAGGGGAAAC

CCCGAAGAAC

GTTGAAGCGG

ATTACAAAAG

AAAACAAT'A

AACGTGCTGA

TTAGAAACAA

ACACGTCGTI'

GAACTACTAG

GTTGGTGATA

GATCAAAAAA

GOCAGATATA

ACCGATCTGA

TATTGGATGC

GACCATAAAT

GGCTGTATCA

ATGCCrGGTAA

GTAGATTACG

A.AGTTGTGGT TCAAACGGGG TGGATCAAA-A ATT'TTTAAAA AGGCATI!CAA AAAAGAACTA CAAGCAAAAA TGCGAGTATC ALAGGCACAGT AGTGGACACA GAAAAGGCAA CTATCAATAT AGGATtAAAC GTTAAAGGCA CAGTTGATAT TAATGGTGAA ATGATGATGG CACAATCAAC CATATGTTC TGCTAATCAA TTAAAAACAA CGGAAGTTAT TAAACGCGCT GAATAGCCAA CCAAAGAAAA TAGTAAAATr TGTATIGTTT CATTGATGGC ACAAAGGAAC CGCAACAGTA ATTTAGAAGG TCAAATCCTT CG'ITATTAAG TCAAGGGGGC TGGATAAGTA TGGCGATAAA ATACGCCAGG AACAGAAGT TTAP'FrATGC ACATATCTAT GCGAAGAAGT AT a **a a a

S.

EF090-4 (SEQ ID NO:344) TKVNG VSVGWLNVNA AEEKLAQVNQ TEEVVVQTGT KEEKIQrPKK( YQLDQKFLKD H LHS SKVKL P RLNQQI IADV

VAFDKTQIQN

WS IDGAKTQE K IVTTDVITG

VTQIGIHDSD

LNEAFKKELE

EAGKGNYQYN

VLNDDGT INK

LLVNALTNSQE

RYNKGTATVP

HKLDKYGDKE

AKLATLSFPE

AKDFYKAPE I

EKLTTWVTQL

QTNAITAPLV

GFHTILYRTT

AFKTDAGSNG

GKPSYXNASIR

TKED)QTLKA?

ETTYGSANQP

GDTKENSKIA

DVNLEGQmfLD C INTPGTEVS

RGNGTFEIVP

LT-TLN'NKLNX

VLFTDVHGT]T

NNYIEIDLKD

G~sRYSVPVKY

KIFDVSYDGM

EEQGTVVDTQ

TITVDINGEI(

RRFKNNGSYG

QKMYCFIDGX

WMPLL.SQGGV

PVIIYGHIYD

1 184 TABLE 1. Nucleotide and Amino Acid Seqeuences; of E. faecalis Genes.

DAPGEFDKPV DYGEEV EF091-1 (SEQ ID NO:345)

TAATTGGNGG

GGAACAGCAG

GATGATTTAT

GCTGTTCAAA

GATCAAGCCT

GCACAAGGTG

GATCAATTTA

GCCGAAGATG

GAAACTGrI'T

GCTGCAGAAG

GGGTAA

AGATTTITAT

CAGCCGI'GC

CAAATCAAAC

AAGGAACAGA

CTCXIT='GC

TTTCTGCCGA

AAAAAGCAGC

CAGCAGAAGA

CTGCTGCTCT

CAAAAGAAGA

GGCTAAAAAA

CGCATTATI'A

AGAMTC TA

ATTAACAGAA

AGTCTGTC

CATGCTTGAT

TGACGATGCT

TATCTATATT

CGATrCAAGCA

TGTTA.AAGAT

GGCGGATTTT

CT~1'GCACCAA

AAAAACAAAG

ATCGC:AAAAC

AAAGAAAAAA

AACTTAAAA

CAAGATCACG

&AE:GTTAAAG

ANAGAAACCA

GCAGCGAAAG

TCTTAGGNGC AGTAATTCT AATCAGGTAA AGAAFI'ACGT CCCAACATTA CACAGATTAT AAAAAGCCGG CGTTTTATCA CAAAAGATTC ATTGGATAAA AACAAACAGG TGATTTATCT CAGAAGATTT AGGTGAAATT ATTCTGCGGC AGCGGCCAAA CCAAAGATGT TCCTGAAAAA ACGTAAAAAA AGAATTTAAA EF091-2 (SEQ ID NO:346) MAKKG GFFLGAVIGG TAAAVAALLL APKSGKELRD OLSNQTDDLd( NKAQDYTDYA VQKGTELTEI AKQKAGVLSD QASDLACSVK EKTKDSLDKA QGVSCDMLDN FKKQTGDLSD QFKKAADDAQ DHAEDLGE IA EDAAEDIY ID VKDSAAAAKE TVSAGVDEAX ETTKDSIPEKA AE-AKE-DVKDA AXDVKKEFXG EF091-3 (SEQ ID NO:347) AT CAAATCAAAC AGATGATTTA AAAAACAAAG CGCAAGATTA CACAGATTAT 99 9 9 99 99 9 9.

9.

9 9 .9 99 9 0 9* 9. 9 09* 9 9 9.99 9 9.9.

99 9.99 9 .99.

9 9

S

09 9.

GCTGTTCAAA

GATCAAGCCT

GCACAAGGTG

GATCAATTTA

GCCGAAGATG

GAAACTG~T

GCTGCAGAAG

GGGTAA

AAGGAACAGA

CTGATTTGGC

TTTCTGGCGA

AAAAAGCAGC

CAGCAGAAGA

CTGCTGGTGT

CAAAAGAAGA

ATTAACAGAA

AGGTTCTGTC

CATGCTTGAT

TGACGATGCT

TATCTATATT

CCATGAAGCA

TGTI'AAAGAT

ATCGCAAAAC

AAAGAAAAAA

AACTTTAAAA

CAAGATCACG

GACGTTAAA.G

ANAGAAACCA

GCAGCr-AAAG

AAA.AAGCCGG

CAA.AAGATTIC

AACAAACAGG

CAGAAGATTT

ATTCTGCGGC

CCAAAGATGT

ACGTAAAAAA

CGTTTTATCA

ATTGGATAAA

TGATTTATCT

AGCGTGAAATT

AGCGGCCAAA

TCCTGAAAAA

AGAATTTAAA

EF091-4 (SEQ ID NO:348) SNQTDDLK NKAQDYTDYA VQKGTELTEI AKQKACVLSD QASOLAGSW±( QFKKAADDAQ DHAEDLGEIA EDAAEDIYID AEAXEDVKDA AKDVKKEFKG EKTKDSLDKA QGVSGDMLDN FKKQTGDLSD VKDSAAAAKE TVSAGVDEAX ETIKDVPEKA EF092-1 (SEQ ID NO:349)

TAAGGGGATG

AGCACGAGTT

GACATCTG

GTTTTACTTT

CCAAAAGGGA

AAAGTrAG AAGAAAAAkAT

TATTAGTAAC

CAGTATCGGC

TAGGTCCACA

TTCCT'ITAGA

ATCAAGCAAT

GGCAAAAAAA

AAAAATCCAA

TTCTGAAGCA

AGTTCGTTTC

TGTAATTAAC

CTCATTAATG

ACAATTATGT

AAAGCACCAG

GATACAAACT

ATGAAAGGGC

ATGGCACATT

GGATAA

TAGTTTGTTC

AAGATCGTGG

TG-GAAAATAA

AATTTGAACA

ATGGCATICAT

CGCAGGAATG

CATGGAAGCA

AGAGGTGAAT

AAAATTACAA

GAATGGCGAA

EF092-2 (SEQ ID NO:350) MAKKT IMLVCSAGMS TSLLVTKMQI( AAEDRGMEAD IFAVSASEAD TNLENKEVNV 185 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

LLLGPQVRFN K<GQFEQKLQP KGIPLDVINM ADYGKNIGEY \TLCQAISLMG EF092-3 (SEQ ID NO:351) AC AAGATCGTGG CATCAAGCA GACATCTT-TG CAGTATCGGC '-'CTC;AAGCA GT=PTAC'-TT TAGGTCCACA AGTTCGTTTC CCAAAAGGGA TTCC AGA TGTANITAAC AAAGTTTTAG ATCAAGCAAT CTCATTIAATG GATACAAACT TGGAAAATAA AC2AGCTGAAT ATGAAAGGGC AATTTAACA AAAATTACAA ATGGCAGATT ATGGCATGAT GAATGGCGAA

GGAT

EF092-4 (SEQ ID NO:352) EDRGMEAD IFAVSASE-AD TNLENXEVNV LLLGPQVRFN KGQFEQKLQP KGIPLDVINM ADYGMDGEK VLCQAISLMG EF093-1 (SEQ ID NO:353)

TAGTTT~TT

TTCACGGCCA

CGTCAG ITAA

TTTCA'IITAT

CTCGTATACG

GT'ITTAITAA

AAACGAAA

CCGATAAAGC;

TGGCCATTGT

CACAAAAATT

TTCTTCCACA

CGCAACTGAC

ACAAATCTGCG

CAATACTGGA

GAGAATTTTA

TTATITGATG

TACGCAAGCT

TGTTAAACAA

CCTCGATTAT

TCGAAAATAC

ATAG

ATGAGGCAAA

AGTTTTCTCG

ACCCAGGAAT

AATAGACGAA

ACATACAAAA

CAATATCAAG

AATA17CAGG

CCCTTCAGTT

ACTATGCAGG

AGTTAAAAAC

ATGAACAATT

AGAGAGTTTC

AAACITATTG

ACTAGAAGAA

GAAAAGTATC

AGAAGAAAGG

AAGAATAACT

TCATCAAAAA

EF093-2 (SEQ ID NO:354) M RQKYSGNLLF TAMAIVYLMS FLALQLLEER QLTQKFTQAT QEYYAGKSIF HLFLADVKQN RRKLKTEERL VYAQVTLDYT YKNEQLRITV LLNKSGRXYQ YQERVSHQKK

AETILE

EF093-3 (SEQ ID NO:355)

S.

S. 0

S.

OS

e S 0 Se 05 S C

S.

55 S S .50 0 05 S S 500 6 CCTTCAC'IT ACTAGAACAA CGTCAGTTAA CACAAAAA'rr TACGCAAGCT TTTCATI 2AT TTCTTGCAGA TGTTAXACAA CTCCTATACG CGCAAGTGAC CCTCGATTAT GTTTrTATFAA ACAAATCTGG TCGAAAATAC AAAGCGGAAA CAATACTGG

ACCCAGGAAT

AATAGACGAA

ACATACAAAA

CAATATCAAG

ACTATGCAGC GAAAAGTATC AGTTAAAAAC AGAAGAAAGG ATGAACAATT AAGAATAACT AGACAGTI'C TCATCAAAAA EF093-4 (SEQ ID NO:356) LQLLEER QLTQKFTQAT QEYYAGKSIF HLFLADVKQN RRKLKTEERL VYAQVTLDYT YKNEQLRITV LLNKSGRKYQ YQERVSHQKK

AETI

EF094-1 (SEQ ID N'3:3S7) *055 5 e.g.

S. S 5S 00

TAAACATTTG

ATTAATTATA

TTAAGTCAAA

CACCCTCAAG

=IAGCGATG

GCAAAACAAA

AGACATTCAG

TGAAAAAAAC

CAATTGGCGG

AGGTAACAAT

GCAGCACGCT

CAGTATTqTG AGGTGAATGT CTCT'TTrA AACATTAAA AATTGGTCGT AACGATTGGT CCTACGXITC TCATI'ATGAC GTAAGTAAAc CTCACAACGT 'ACTACGTCAT TATTGAACCA GGTGTTAGTA

TTACTCAAAA

TATTTGCGAC

CTTTTiCCCGA

TGTATIGAAGT

TATATGCAGA

TTCCAACACA

ACGAAAGGC

2TI'GGCTCTA

TGAAATTACT

TGACGGAACT

ATACAATGGT

AGTGACGCAC

186 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

GGTTATCAGA

TGGGAAAAGG

GAAGTGAACG

AAATCTATTG

CATAATACCA

TTAAATTTAT

ATTGGGAATC

AAAAAATCAG

ATGGCTGGTG

GG3TTC'PTTAA

TCCATTTAG

A=TCTATTT

CCAGATCCTT

ATTTCCATGA

GTAGAAACAG

ATTCGTAAAG

CGTGCGGAAA

GAAACTAAAT

TATGCCAATC

ACTGGGGAAA

GCTGAGTITA

TGGAGTGAAG

ACTTTGGCTT

TTCTGGCAAG

TCCATCAAAA

AAAGAACAAG

ACTGCCCAAA

GAAATCACAG

GGCTATGGTA

CCAGAAGGAT

GACCACTATG

ATTAAGATGG

AACCGITGA

TGGAAACACG

AGATATAACT

GAAGCCACAA

ACAACCCCAA

ACCGCTGAAC

GTAGCCTTTG

CAAACCCTCA

GATGGTTCGC

ACCCATGATG

CCAGATGGTA

AAAGAATTTA

ACTAAGTTTA

AATGAAGAT

AGTACGCCAA

ACAGTGAAGA

TTTATCTTGA

AAAACCCTTT

CTGCAACAGA

GTTATAAACT

AAGGAAAAAT

CTGTAAAAAC

CTGAGTTTGA

AATTAGTGCT

CTGGTACTGG

CGCTTGATAA

AGATCAAAAA

ATT'flGGGAA

TGGATGGAAT

ATATGATTGA

CTTCGAAAAA

GTACTGATCT

ACAGCCCAGC

CACCAAAAGA

CTAGTAATGG

AAACCGTGC

CCACTTTGAC

AAGGAGCTGA

CTTTTAAAAC

TAGATGAAAA

AAACCAAAGC

AAGTTGATAA

TATTCGCTT

CTGGArPITAA

GTGCTGAACA

AGTTTGAAAA

TTCAAAAGAT

CGAAGACTGA

TGACCGTI'CC

TGCTTTATGA

GAAATAAAAA

TGCATGAAAT

AAGCTGCCCA

CGTTr.CGAA AAGTrTTGrGA

AAGCAGGCAA

A'rrGTACGAT AAACT LTrAC

TACTGGATGG

CGAACAAAGA

CCAAAACCGT

CTTTITACGGA

TGAAAGAAAA

AACAACCGGA

CATTCCCGCA

Trr TCGAC

GCCATCAATG

TATTGATACA

CCATT-CATA

TAATAAGGCA

AATTTTAGGT

TAAAGTCGTC

TACTCCAAAC

AACTCCAGTC

GCCCAATACC

AATCGATAAA

AGCTTTACC-T

TCCCCATGGrT

TACCACACCC

TATGCGACAA

TTrGGAATCAC

TGGTGAAATT

GCTTGCTAAT

TTTCGTCAAT

TCTTGTTACC

AAAAGAAGAC

ATATACTCTC

AGAATTAGTG

GAACCAAGT

ACCTGAAGGA

TAACGAAAAA

TGGC=TGAT

CGACTTATCT

TAAAGCGACC

TCTTCCTTAT

TACACCACTA

GTATGTCITT

TTACGAGAAA

n=GCCCGAG

ATTCAATACC

CAAAGAAGAC

AGAAAAAGAC

CAAAGAGAAA

TAAAAGCATC

TGAG.CCAGtA

TGAACGCCAT

TCATGGTGAC

CTCAAAAGAA

AATTTGGAAA

ACTTGCGCAA

AATCAATTAc

ATCTCAAACC

AACACCAGCT

AACTGGGGAG

TGCTGGGTAT

TCTOATAAAG

AATATGGTTG

AAAAGATTAG

ATTGAGGAGT

CAATCGACAA

CAAkAATACGG TCTAAtTCCA

GCTTATAAAA

TAC'-CTAETA

GAATCAGGTG

TCAAAAGATG

kWAAGGTAA

ATGGCTGCCA

AAAGGTCAAA

A.ATI'ATTCTC

GTCCAAGA;A

GCITTTGGAAC

ACCI'CAAAC

AGTAACGTAA

AAAGATACCG

TTTACTGCAA

AAGGGA.ACGA

GCCGTTAAAC

TATACT=G

AATGCCGTAA

TTCTTTAAAT

TTTAAAGTGT

ACAGCTI'GTA

GGGGATTATT

GAAATCCGTT

ACCATTACCG

CTAACTAACA

AAAGAAGATA

CTTGAT"TTA

TCCTATGTCG

GAAAA.AGCCA

ACTGGAACTT

GTCTTGTTCA

GCGAAGGATG

GVF1'CCATCC

GTOATGGATA

GCrrCGAAA

TCTGGCAAAA

AAAGTAGATA

GAAAAAGATC

TTAACACCAA

GTTCCAAGTA

AAAAA.TTCCA

TATTTCTGGA

CGAAACTAGT

CACAAAAGAT

GT GCTGCTTC

ATCAAAAAAA

CT=AATAGA

CGAATATAGA

AATCAGGAAC

AAGCAGGACT

AAATTAATGT

ATATTGTACC

TGACAACAGA

CTATTACTGA

CCATTAAAGC

TTCTTTTAGA

TAGCTGGAAA

TAACAACQGA

TGGGAACCTA

CAACAAAAGT

AAGCGCAAAA

GTA-ATGAGAG

AAGATGGTCA

A.AGCTTCTGA

ACCTAGCAAT

ATGAAACGAA

TTACTCGAGA

TTCTGGATC

.CGCCATTGGA

ACGAGCAATT

TACTTAAGA

CTACATTTAA

AAGA.AGGACA

ACGAGIMrT

GTTGACTT'C

CCGAGCTAGT

TAGCTCAAGC

AACCGGTGGT

r-GAAAATTCT AT-TATGTr.TA

CTAGCTTGAA

AAACAAAAC

TGT=rATGA

CAATCTGTA

TTGAGCATGA

CCGGAAAGTA

GAA.ACGTGAA

AAGAAGTGCC

ATTCTCAAGA

ACGIT=-ACT

ATCGCCGCAA

ATCGGTTCTT

GATTGGGAA

AGTTGATATA

ACCAAGTI'TTT

TAAAAATGAA

TTACCGTrGTA

AT'ALACATTG

TCAA.ACTGTG

GGAAACTAAG

AGAAACGGTT

TAAAGATGGG

AAAATCGGTG

GGGCGAGACC

GAAGACTGGG

TACATTTGCC

TGAAAAAGGT

TTACGTGACA

CGAGTTAAAA

CCAAGAAATT

TCAAGGGAAA

AGCTGTTAAA

TrCAAACAGTG

TAACGAGTAT

GTATCCTGTA

TC-TTACGGCA

G'CCTGATGGC

AGGGACCAAN

ACGGI'rTMAT

AATAGAGGCT

GGAAAACAAA

AAAACAACCA

TGTTAGTCTG

TCTTGCGACT

TGATAAATTG

CATTGATCTG

GATTGCCGA.A

GCATAAATTA

TGAAAACAAG

CAATCAAGCA

CCACCTAGAA

TGTGTCGGTT

TGCTTTACTA

AGTGAATGAT

TCCAGAAGGA

TGGAAAACAC

AACCATACCG

ATCTAGTCCC

GTI'AGTTGGC

T1'AA EF094-2 (SEQ ID NO:358) MKXTTFKN WsLFATLALL~ SQTIGGTIGP TIAFADEITH PQEVTIHYDV SKLYEVDGTF SDGSTLSERT TSLYAEYDEA KQTVFCIEPG VSIPTEVTHG TABLE 1. Nucleotide and Amino Acid Seqeuences of Efaecalis Genes.

YQKNPLPSMS

SIEGKINKAI

GNQLVLTPNS

SLK IKIDKE

DPYMIDTTPM

RXDS PAGEIV

ANQTVALVTS

SEAFKTELVK

IKKVDNNEKN

ITGAEDKATT

DYAKSEYVFT

KDGNKKLNTL

TATLANKEKT

TVNCTIER-W

DGTNKEIWKS

EDLKEKSQTL

ILIFSTAGYY

DKAKLVSVLW

EEYQKKPSFH

NSKSGTLTLK

SGDIVPETVF

AATIKAGETI

QEITTDEKGR

NVKGQNQEIT

GTKASDEIVT

AVITRDVTAK

ACNEQLGFDG

ITEEGQKQP I

OFTELVDKLR

GTWKILHKLT

S IQTKAHLED

GKIEHEVNDK

TPKEVPTI PS

FWNRRN

EKAGTDIDTN

NT'IVKTI LGQ

KSAGTGTPVA

HLDFGKALPS

SMTSKNt4RQK

AETPKELANA

GETTLTKEDK

LALDEKNQVA

EQVIRFGFDF

YGKFENLPYG

KIIVTVPYEKL

YNLHEIKEJW

AEQVLDKSIV

GSQTFTHGDV

EFTKTVLAEI<

TPKQP-TTPAV

MVA QKMIWEE ST'LI DKDIEL

YKXAGLQTVM

KDV'ITDKDGI

GQ ILLEKTOV

LELGTYYVTE

DTGNESQGKA

VKRHLAINEYF

FKFAG-SADCT

DYLLEE IEAP

TNNEFSVSLN

YVVAQAI DVE LFNYVYENflV

MOMFDDVSVT

VDTGKYPEGT

PSNSQESSPT

VINGYXLHS 1K RLGGASVDIK NLSEFDKVVQ NTANIDYRVI AGALDKPNTY AIKI NVETXG SILDGIPHGT KVTITEXSVP ETGTDLWND)N YSLAGN'TFAI TrKS SNGFVNT FKPTKVELKY EFKGAEYTLF TAKDGQAVWW WQETXAPEGY TLDETKYPVS AETGFNDLSF KVSPLEGTXE EGFQKITPLE I RSTFKENKD RLMLYDLPEK EDSLTSLATW ATKAAQEI<DE KAXPVV IAET AFEAGNEPVA KDASLNNQAQ HD)VLDGSKEA FETILYALLP YXFTFTEINYE KDGNVNGKH-N VKTFPQTGEK NSNVLLLVGF' EF094-3 (SEQ ID NO:359) CGA TGAAATTACT CACCCTCAAG AGGTAACAAT TTTAGCGATG GCAGCACGCT GCAAACAAA CAGTATTTTG GGTTATCAGA AAAACCCTTT TGGGAAAAGG CTGGAACAGA GAACTCAACG GTTATAAACT AAATCTATTG AACGAAAAAT CATAATACCA CTGTAAAAAC TTAAATTTAT CTGAGTTTGA ATTGGGAATC AATTAGTCCT AAAAAATCAG CTGGTACTG ATGGCTGGTG CGCTTGATAA GGTTCTTTAA ACATCAAAAA TTCCATTTAG ATTTTGGGAA ATTTCTATTT TGGATGGAAT CCAGATCCIT ATATGATTGA ATTTCCATGA CTTCGAAAAA GTAGAAACAG GTACTGATCT ATTCGTAAAG ACAGCCCAGC CGTCCGGAAA CACCAAAACA GAAACTAAAT CTAGTAATGG TATG.CCAATC AAACCGTGGC

TCAITATGAC

CTCAGAACGT

TKPI'GAACCA

GCCATCA-ATG

TATTGATACA

CCATTCCATA

TAATAAGCCA

AA'TTAGGT

TAAAGTCGTC

TACTCCAA-AC

AACTCCAGTC

GCCCAATACC

AATCGATAAA

AGCTTTACCT

TCCCCATGGT

TACCACACCC

TATrGCGACAA

TTGGAATGAC

TGGTGAAATT

GCTTGCTA-AT

TTTCGT13AAT

TCTTGI'IACC

AAAACAAGAC

ATATACTCTC

AGAATTAGTG

GTAAGTAAAC

ACTACGTCAT

GGTGTTAGTA

TCTGATAAAG

AATATGGTTG

AAAAGATTAG

AT1=AGGCT

CAATCGACAA

CAAAATACGG

TCTAATTCCA

GCTTATAAAA

TACGCTATTA

GAATCAGC9.G

TCAAAAGATG

ACAAAGGTAA

ATGGCTGCCA

AAAGGTCAAA

AATTATTCTC

GTCCAAGAAA

GC'rTrGGAAC

ACCTTCAAAC

AGTAACGTAA

AAAGATACCG

TTTACTGCAA

AAGGGAACGA

TG-TATCAACT TGACGGAACT TATATGCAGA ATACALATGGT TTCCAACAGA AGTGACGCAC CGAAACTAGT ATCGGTTCTT CACAAAAGAT GATTTGGGAA GTGGTGCTTC AGTTGATATA ATCAAAAAAA ACCAAGTTTT CITTAATAGA TAAAATGAA CGAATATAGA TTACCGTGTA AATCAGGAAC ATTAACATTG AAGCAGGACT TCAAACTGTG AAATTAATGT GGAAACTAAG ATAF1'GTACC AGAAACGGTT TGACAACAGA TA.AAGATGGG CTATTACTGA AAAATCGGTG CCATTAAAGC GGGCGAGACC ?I'CTI'TAGA CAAGACTGGG TAGCTGGAAA TACATTTCC TAACAACGGA TGAAAAAGGT TGGGAACCTA TTACGTGACA CAACAA-AAGT CGAGTTAAAA AAGC-GCAAAA CCAAGAAATT GTAATIAGAG TCAAGGGAA-A AAGATGGTCA AGCTGTTAAA AAGCT1'CTGA TGAAACAG

ACTGGGGAAA

GCTGAG'FITA

TGGAGTGAAG

CCACTTTGAC

AAGGAGCTGA

CT'ITAAAAC

EF094-4 (SEQ ID NO:360)

DEITH

PQEVTIHYDV

YQKNPLPSMS

S IECKINKAI

GNQLVLTPNS

SKLYEVDGTF SDGSTLSERT DKAKLVSVLW EKAGTDIDTN EEYQKKPSFH NTI'VKTILGQ NSKSGTLTLK KSAGTGTPVA

TSLYAEYNGA

MVAQKMIWEE

STTLIDMTL

YK(AGLQTVM

KQTVFCIEPG VSIPTEV'rHG VNGYKLHS 1K RLCGASVDII( NLSEFDKVVQ NTANI DYRVI AGALDKPNTY" AIKINVETKG 188 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faeca (is Genes.

SLKIRKIDKE

DPYMI DTTPM

RKDSPAGEIV

ANQTVALVTS

SEAFKTELVK

SGDIVPETVF

AATIKAGETI

QEITTDEKGR

NVKGQNQEIT

GTKASDET

HLDFGKALPS

SMTSKNMRQK

AETPKELANA

GETTLTKEDK

KDVTTDKDGI

GQILLEKTGV

LELGTYYVTE

DTGNES QGXA S ILDGI.PHGT

ETGTDLWL\DN

TKSSNGFVNT

EFKGAEYTLF

KVTITEKSVP

YSLAGNTFAI

FKPTKVELKY

TAKDGQAVKW

EF095-1. (SEQ ID NO:361)

TAAGAATT

GAATTGATAG

GTT=PTGCAG

ACAGCAGAAA

ACACTGGCAT

ATTAGAGAAA

GGGCAGCCAG

CCAGATGGCA

AATI'TAAAAG

GTGCTTGCAG

TACAATAAGA

GGAALACCAAT

TATAGTCAGA

TTGCTACAACC

TTTCAGGGC

ACTCAAGGGA

GACTGACC

TCAAAAACGT

AAAAGCGTAA

TATAAGGAGA

CCACCATCTC

TTAGCTGGAA

GGTTGGTATC

TCCCCTGTTT

GCTGAAATGC

CAGATTATGC

AGTGGTTGGT

CCAAACAAAA

GAAGTGCCTA

CATCAAGTCT

GATACTGTCA

ATCAGCACAC

GGTTTAAAGC

AAAAAATCAC

GATCAACTAT

CAGTACAATC

GTTGCCAACG

TATCAATTTG

AAAGATCAAA

TGGATTGTTC TTTACAAAGA

TAACGGGCAT

AAACATTACC

AAGCCGAAAG

TGTCAAAAAG

GANI'rGAGAC

TAAACGCCAA

TAAATGTrGTC

AAGTGGTAAT

CGAGTAATCA

AACGGGAAAT

ATCCAACAAC

AAACAGGAGT

AAGTGAAGGT

CGGTTATTA

AACCAAT'CC

CTTACACC'Fr

ATCAATTTCA

CGCCCAGTTA

TACCTCAAAA

ATTTIATTCA

AAAAACTGCC

AAGATAAAAC

TTAATGAAAT

AAATAGAAGG

7rACAAATGT

CACCAGGTCT

TTGTTCCTAT

TTGGTCAGAC

TACAAGCGGC

GAATCCAACC

CAACTT'ITGA

AGGCAGCAGA

AACCCAATTG

CATCAATGAC

AACCAACGGA

GTGTAGCTAG

ATTTITCTTT

CTTATCAAGC

CTGCCATATA

AAGTACAAAA

TGAACAACCA

TGAGTTAATC

GCCTAACCTA

TGAGATCT

GGAAGGTGAA

TCCAAGTGAG

AACAT=rT-I

TGATCCCAAT

AATTTCGCAA

AACGTTTAAC

TCATTCATCG

TCATGTTACC

TCCACCACCG

TAAACAGAAA

AGGATGGTAT

TGATATTACC

AAATTATACA

GGATCACCAA

ACAACAATAC

NAAACAAGAG

GAACGCTATI'

ACTAGTCAAA

GGGAGAAGTA

AGCTCGGCCA

TACTGATGAA

AGCAACTATT

TGTTOAAATC

TAAAAATCAA

TITTTGGCAAA

'ITA'ITATCAA

GGCACTAACT

AAAG ITAUTG

AACTAAACTT

CCATA=TGTT

TGATCAAATC

AATGGTGACT

Cj .AGTAAGAG

CAGACAAAGG

GATAATrGAGr, ACTTATCG~r

CTACAGTATC

AGTCAACCAG

AAAGTAGCCG

TTACCAAGAT

TATCCGCTC

TTTGAATTGG

ATTAGCGAGA

AATCAATCTC;

AATCGCAAAG

GGGTTTAGAC

GATCTTTTGC

AAAGGGAAAA

TATGACGACA

TTTG;AGGAIM

CAACCAATAA

AGCGTTAACG

AGCTTAGAAA

ACAGCAC=G

GTCATGCCAA

CCGTTAAAAA

ATCCAAGTCA

AAL'IGGCGAG

ATGATGACAA

AATGGAAATT

GAAATTGTGG

GTCGCCAM1

AATGCTCAGC

GCAGAACTAT

TTAGGAACAA

GCTCTTGGCA

GCCAATGGTC

AAATI'AGAAA

TGGAATTAG

AAGGAACCAA TCATTCATTA ATAA-ACTACA TGATGAAGAA CTAATGTTAC AAGTCAAACG ATGGATTAT TAATGAAGAG ATAGTTGGCA AGGCAATTCC TGACAGCATC TACAGTGGCT TCTAITCCGA CATGTCAACG ATTATACTTC TTAAGCTTA CAACTATTTC CGACGCATCA AAAAAATGTC TGCACAACAA GTCAAAAACT AATCG'ITCCT GCCTA'ITGAA TTACTTTAAA TGACAGAACA 'I=TGTGGAT AAGGAAAGCA AACACTTATT CAAGTAGCTA TGAAAWI'GAC CGAAACCTGA AAATTTAGAA ATGATGATTT AACTGTTGTC TCAATGGTGT TGAAATTGCA CTACGGATGG CTTTCGCTAT GTAAAACTTA TTTATATCAA AAACGAAGCG ACCCATAAAC ATAAGCAAAT AACTGCAAAA GTGGTTATAT ACAAATITG AAATAAACTT AAAGCCAGCA CCATTCGTGT TGGATCTGAA TTGGCArTAC TTTAAATACG CAATTCTAC AGGTGAACCA TTTCTCCTGT TCACGCACCT CACCAGATGA GGAAGGTTI' CTAGCAACAC GCAGCAACAT AAAATCCATA =~ACGTTTG CCCCC 1TTCA AGGAAGAGTG CCAATG=ITC AGGTTTTATT AAACAACCGC TATTCAATI'A AGTITGrACGA AAGTGATGTT TTCCAGCAAA TCAAGGTAGA TGACAGGCCC ATAA AGGGACA.ATA TGAAGCGAAG TAAATGGAAA ?I'AGTATTCC CCATACTA-AT ACACACAACT 0 EF095-2 (SEQ 10 NO~:362) MKRSKWKE LIVTGICHIL VFPILIQTTV FAETLPSTKQ VR.EGTN14SLT AEKAESEQPQ TKDKLHDEET LALSKSELID N~EANv'rSQTI RERIETPNLT YRYGFINEEG QPVNANEILL QYHSWQGNSP DCINVWECGES QPVTAS TVAN LKEVVIPSEK VAVYSDMSrTV 189 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.f[aecalis Genes.

LAASNQTFFL

SQKTCVTFNI

QGKPIPPPPG

SVTPSYCITY

AGKKLPQQYS

EMQIEGLVXV

NKIVP ITDEN

TVRIQPKNQE

KSQPNWALTA

ANGVASHIVA

PRYYTSLSLY

SESQKLIVPL

FRQGKQTILIE

DDNDDLTVVY

VNGKTYLYQG

MPSGYIQIWQ

WRVGI TLNTv- IVAPDEEGF I ELS PFEGRVD NGQF[7ESDVY NKXGEI DPNY

YNQVKVDSSN

RDPYTFKQKD

KEI PQKNYTF

WYQDKTKQES

IMLTNVGEVP

VPIGQTATIM

STPTFDFGKV

QLSSMTKLLL

QFDFSFDQIK

PLPTISDASG

QSGLLNYPI(P

LLPSSYEIDS

EDVNGVE lAP LEKTKRP INS

LKKINLKPAS

MTTIATGEPD

AISSNTQQHG

G;TTNVSG;FIQ

LE I PAQGRK

NQYPTTISQF

SC? VYYHVTN

KTYQFQGWYK

PSDFIQCHQQ

PVF-NEL'NAIT

GtAJSPGLARP I

QVLQAAVEI~

LKQA.ADYYEN

YNQPTETKVA

DQTYQAMVTW

ELEKMdSAQQY

RKVTEHFVDT

GKTKPENLEK

P ITI'DGFRYL

AVYKEITAKA

QVTIRVGSEP

GNFSAVHAAD

GQENPYLRLK

LGKTTAIQLV

NLVTGP

EF095-3 (SEQ ID NO:363) *5

S

AAGTACAAA.A

ACAGCAGAAA

ACACTGGCAT

ATTAGAGAAA

GGGCAGCCAG

CCAGATGGCA

AA IIAAAAG

GTGCTTGCAG

TACAATAAGA

GGAAACCAAT

TATAGTCAGA

TTGTACAACC

TTTTCAGGGC

ACTCAAGGGA

GAGCGTGACC

TCAAAAACGT

AAAAGCGTAA

TATAAGGAGA

CCACCATCTG

ITAGCTGGAA

GCTTGGTATC

TCCCCTGTTT

GCTGAAATGC

CAGATTATGC

AGTGGTTGGT

CCAAACAAAA

GAAGTGCCTA

GATCAAGTGT

GATACTGTCA

ATCAGCACAC

GGITI'AAAGC

AAAAAATCAC

GATCAACTAT

CAGTACAATC

GTTGCCAACG

TATCAATTTG

AAAGATCAAA

CAAGTAAGAG

AAGCCGAAAG

TTCAAAAAG

GAATTGAGAC

TAAACGCCAA

TAAATGTGTG

AAGTGGTAAT

CGAGTAATCA

AAGGGGAAAT

ATCCAACAAC

AAACAGGAGT

AAGTGAAGGT

CGG TTA

AACCAATCCC

CTTACACCTT

ATCAATTTCA

CGCCCAGTTA

TACCTCAA;A

ATTTItTTCA

AAAAACTGCC

AAGATAAAAC

TTAATGAAAT

AAATAGAAGG

TTACAAATGT

CACCAGGTCT

TTGTTCCTAT

TI'GGTCAGAC

TACAAGCGGC

GAATCCAACC

CAACTTTTGA

AGGCAGCAGA

AACCCAATTG

CATCAATGAC

AACCAACGGA

GTGTAGCTAG

ATTTTTCTT

CTTATCAAGC

AAGGAACCAA

TGAACAACCA

TGAGTTAATC

GCCTAACCTA

TGAGATCCTT

GGAAGGTGAA

TCCAAGTGAG

AACATTTTT

TGATCCCAAT

AATTCGCAA

AACGTTTAAC

TGATTCATCG

TCATGTrACC

TCCACCACCG

TAAACAGAAA

AGGATGGTAT

TGATATTACC

AAATTATACA

GGATCACCAA

ACAACAATAC

NAAACAAGAG

GAACGCTATT

ACTAGTCAAA

GGGAGAAGTA

AGCTCGGCCA

TACTGATGAA

AGCAACTATT

TGTTGAAATG

TAAAAATCAA

TTTTGGCAAA

TTATTATGAA

GGCACTAACT

AAAG ITATTG

A.ACTAAACTT

CCATATTGTT

TIGATCAAATC

AATGGTGACT

TCATrCATI'A 0 CAGACAAAGG ATAAACTACA TGATGAAGAA G.ATAATGAGG CTAATrGTTAC AAGTCAAACG ACTTATCGTT ATGGArI'TAT TAATGAAGAG CTACAGTATC ATAGTTGGCA AGGCAAITCC AGTCA.ACCAG TGACAGCATC TACAGTGGCT AAAGTAGCCG TCTATTCCGA CATGTCAACG TTACCAAGAT ATTATACTTC TFrAAGCTTA TATCCGCTGC CAACTATTTC CGACGCATCA TTTGAA=IG AAAAAATGTC TGCACAkACAA ATTAGCGAGA GTCAAAAACT AATCGTTCCT A.ATCAATCTG GGCTAITGAA 'ITACTTTAALA AATCGCAAAG TGACAGAACA TTTTGTGG.AT GGGTTTAGAC AAGGAAAGCA AACACTTATT GATC'I"'TTC CAAGTACCTA TGAAATTGAC AAAGGGAAAA CGAAACCTGA AAATTTAGAA TATGACGACA ATG3ATGATTr AACTGTTGTC TTTGAGCATG TCAATGGTGT TGAAATTGCA CAACCAATAA CTACGGATGG CTTTCGCTAT AGCG TTAACG GTAAAACTTA TTTATATCAA AGCTTAGAAA AAACGAACG ACCCATAAAC ACAGCAGTC'r ATAAGGAAAT AACTGCAAA.A GTCATGCCAA GTGTATAT ACAAATTTGG CCG ITAAAAA AAATAAACTT AAAGCCAGCA ATCCAAGTCA CGATTCGTG-T TGGATCTGAA A.ATTGGCGAG TTGCATTAC T=AAATACG ATGATGACAA CAATTGCTAC AGGTGAACCA AATGGAAATT TTTCTGCTGT TCACGCAGCT GAAATTGTGG CACCAGATGA GGAAGGTTTT GTCGCCATTT CTAGCAACAC GCAGCAACAT AATG--TCAGG AAAATCCATA TTrACGTrC GCAGAACTAT CCCCCTTTGA AGGAAGAGTG TTAGGAACAA CCAATGTTTC AGGTTMATT GCTCTTGCCA AAACAACCGC TAT~CAATTA GCCAATGGTC AGT=GACGA AAGTGATGTT AAATTAGAAA TTCCAGCAAA TCAAGGTAGA TGGAATTTAG TGACAGGCCC A

S.

EF095-4 (SEQ ID NO:364) STKQ VREGTNHSLT 190 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. [aecalis Genes.

AEKAESEQPQ

QPVNANEILL

LAASNQTFFL

SQKTGVTFNI

QGKPIPPPPG

SVTPSYDITY

AGKKLPQQYS

EMQ EGLVKV

NKIVPITDEN

TVRIQPKNQE

KSQPNWALTA

ANGVASH IVA

TKDKLHDEET

QYHSWQGNSP

PRYYTSLSLY

SESQKLIVPL

FRQCKQTLIE

DDNDDLTVVY

VNGKTYLYQG

MPSGYIQIWQ

WRVGITLNTE

IVAPDEEGFI

ELSPFECRVD

NGQFDESDVY

LALSKSELID

DC INVWECES

N~KXGEIDPNY

YNQVIKVDSSN

RDPYTFKQKD

KEIPQKNYTF

WYQDKTKQES

IMLTNVGEVP

VP ICQTATIM

STPTFDFGKV

QLSSMTKLLL

QFDFSFDQIK

NEANVTSQTI

QPVTASTVAN

PLPTISDASG

QSGLLNYFKF

LLPSSYEIDS

EDVNGVEIAP

LEKTKRP INS LKK INLKPAS

MTTIATGEPD

AISSNTQQHG

GTTNVSGFIQ

L&I PANQCRK

RERIETPNLT

LKEVVIPSER

NQYPITISQF

SGPVYYHVTN

KTYQFQGWYK

PSDFIQDHQQ

PVFNMAIT

GWSPGLARPI

QVLQAAVEMN

LKQA.ADYYEN

YNQPTEzTKVA

DQTYQAMIVTW

YRYGFINEEG

VA~VYSDMSTV

ELEKMSAQQY

RKVTEHFVDT

GKTKPENLEK

P ITTDGFRYL

AVYKEITAKA

Q VT IR VGSE P

GNFSAVHAAD

GQENPYLRLK

LGKTTAIQLV

ULVTGP

EF096-1 (SEQ ID NO:365)

TGAGGTGCC

ATTGTCATCT

GATACCTTGA

GAAAAAGAAA

ACAAAAATTC

CAAAAAATGA

GAAAACATTG

CCGTACTTCT

ATCCAACATT

GGCGCTCGCCG

AACGACCAAC

CCAATTGTTG

ACTTTCTCTG

CCCAGTGAAG

AAAGAGGGTG

CAGAATATA

GTTGCCGAAG

CA.AGACTTAG

TATCGGAAAT

AAGTTAAAAT

TGTTTITTGG

CCATTTACAA

CAGGCTATAA

AGCAAGGTG

TGAA.AGCGAA

ATG.CACGCTr

GGGGCACGTT

GGGATGATTT

AAGTG ITAGG

AATI'ACGTCA

CCGATCAAAT

CTGAACCTC

GCTCTAATCT

CCTATGCATT

TTGTI'ATTC

ATAAACAATT

GTCAAGAATA

AA

GAAAAAXI'TA

CGTGCGCCAA

TTGGGGGGAC

AGTCAATTAC

CACAGCCTAT

GATGCTTTTA

TI'TAGATCAA

GGGGATTAT

ATGGCGCCCG

ATTATCTIr

GGCTACCGAT

CAAA.ATGTAC

TCAAATCCTA

CTGGTT'GAT

TATGAACTI'

CACACCAAAT

TTATCCAGAT

CTTAGGAAT'

CAGTCACTTT

TTGGAGAAAG

TATATAGATC

GAAACCTTTG

GATATTGCCA

CCACTTGATC

TCCTTTGATC

TATAATGATA

GAATTAAAAA

AACAGTTTAG

AAGTTAAACC

ATGGCTAATG

GAAAACAATG

AACATTGTr.A

ATG'ITACGAC

AAAGAAGCTA

GATGAALACTA

TATALACGATC

TTAT7GGA-AT

CAAGTGGCAT

CGGCCTTGAT

AITCTAATGA

TTCCTTCTGA

ACAGCAAAT

CCAAAAATAA

AATTTATTGA

ATAATGTCAT

GCTATTCGTT

GAT'TAACGAA

AAGAAAGTGC

AACATCTACA

TGCCTAAGAC

CAGAAAATGC

AAAAACTATT

TCA.AACATTT

TGTTC 'rGGA

TATCGCTATT

GGCAGGAGCA

TAAGAAATTT

AGCTATGTAT

ATATATGATT

AAAAGGCT'A

CTTTTCCGTT

CGGCCGTCAG

GCTGATTGAT

AAACACTAA

CAATGTCAAA

AGTTGCTGTA

ITATGTGATI'

AGCCAAAAAT

GGCACAAAAT

ACCAAAAGAA

AGAATT1TAC

GITAAGATG

0.

o 0* *0 0 V EF096-2 (SEQ ID NO:366) MKKLQ SLFIGIIAII VILFFGVRQL EKASGMAGAD TLTIYNWGCY IDPALIKKFE KETGYKVNYE TFDSNEAMYT KIQQGCTAYD IAIPSEYNIQ KMMKAKMLLP LDHSKLKCLE NIDARFLDQS FDPKNKFSVP YFWCTLGIIY NDKFIDGRQI QHWIDDLW.RPE LKNNVMLIDG AREVLGL.SLN SLCYSLNSKN DQQLRQATDK LNRLTNNVKA IVADEIKMYM ANEESAVAVT FSGEAAEMLE NNEHLHYVIP SEGSNLWFDN IVMPXTAKNK EGAYAFMNFM LRPENAAQNA EYICYSTPNK EAKXLLPKEV AEDKQFYPDD ETIKHLEVQ DLGQE-YLGIY NDLFLEFKMY

RK

EF096-3 (SEQ ID NO:367) AAGTGGCAT GGCAGGAGCA CATACCTTGA CCATTTACAA GAAAAAGA.AA CAGGCTATAA ACAAAAATTC AGCAAGGTGG CAAAAAATGA TGAAAGCGAA GAAAACAT1TG ATGCACGCT CCGTACTTCT GGGGCACGT

TT'GGGGGGAC

ACTCAATTAC

CACAGCCTAT

GATGCTTTTA

TTT1AGATCAA

GGGGATTATT

TATATAGATC

GAAACCTIVC

GATATTOCCA

CCACTTGATC

TCCTTTGATC

TATAATGATA

CGGCC=IAT

ATrCTAATGA

TTCCTTCTGA

ACAGCAAATT

CCA.AAAkATA).

AATTTATTGA

TAAGAAATTT

AGCTATGTAT

ATATATGATT

AAAAGGCTTA

GTTTTCCGTT

CCCCCTCAG

191 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. aecalis Genes.

ATCCAACATT

GGCGCTCGCG

AACGACCAAC

GCAATTGTTG

ACITCTCTG

CCCAGTGAAG

AAAGAGGGTG

GCAGAATATA

GTTGCCGAAG

CAAGACTTAG

TATCGGAAA

GGGATGA=T

AAGTG'ITAGG

AATTACGTCA

CCGATGAAAT

GTGAAGCTGC

GCTCTAATCT

CCTATGCATT

TTGGTTATTC

ATAAACAATT

GTCAAGAATA

ATGGCGCCCG

ATTATCTTTG

GGCTACCGAT

CAAAATGTAC

TGAAATGCTA

CTGG=1FGAT TATGAAC'Tr

CACACCAA.AT

TTATCCAGAT

CTTAGGAATT

GAATTAAAAA

AACAGTTTAG

AAGTTAA.AcC

ATGGCTAATG

GAAAACAATG

AACATTGTGA

ATGTTACGAC

A-AAGAAGCTA

GAMA-AACTA

TATAACGATC

ATAATGTCAT

GCTATTCCT

GATTAACGAA

AAGAAAGTGC

AACATCTACA

TGCCTAAGAC

CAGAAAATC

A-AAAACTATT

TCAAACATTT

TGTTCTTGGA

GCTGATTGAT

AAACAGTAAA

CAATGTCA.AA

AGTTGCTGTA

TTATGTGTr

AGCCAAAAAT

GGCACAAAAT

ACCAAAAGAA

AGAAGTTTAC

GTTTAAGATG

EF096-4 (SEQ ID NO:3 68) SGMAGAD TLTIYNWGDY IDPALIKKFE KETGYKVNYE TFDSNEAKYT KIQQGGTAYD NIDARFLDQS FDPKNKFSVP YFWGTLGIIY AREVLGLSLN SLGYSLNSKN DQQLRQATDK FSGEAAE-MLE NNEHLHYVIP SEGSNLWFDN EYIGYSTPNX EAKKLLPKEV AEDKQFYPDD

RK

IAIPS EYMIQ NDK I DGRQI

LNRLTNNV*XA

IVMPKTAKUK

ETIKHLEVYQ

KPMUALLP

QHWD)DLWRPE

IVADEIKMYM

EGAYAFMNFM

D)LGQEYLG IY LDH SKLKGLE LKNNVMLI DG

ANEESAVAVT

LRPENAAQNA

NDLFLEFKMY

EF097-1 (SEQ ID NO:369) a a

TAGAAGTATT

ATGCATTCGC

GGTCATCGTT

ITGTCTTITGG

ACGATAATGA

GAGGAACAAC

GATGTTCCAC

AAAATTGAAC

ITTFTAGCAG

GCTGTTGAA.A

CTTTTACCAT

ATTAACCATG

A~TTTTC

CTGTTTGGGC

GGGGGCATTC

GTAATTGCTG

GCTCCAGCTT

CTGGCGGTTI'

LTATTAAAAC

CAAGTGGAAA

GGCTCAAGTG

ATGCCTGTGA

ArI'CAAGCAG G 1TCAAAATT

TCTTCTCAAG

ATACAGAAGC

GAATTATITGC

CTGGAA.ACAG

GCCTATCATT

AAAGAGTATC

CTAATTATCT

TCTTTTTTAA

TGAGTGGGAT

TGGCTGGCTA

TTCGAGTTGT

GTGGCGGCGT

AGTTGTTGG

AAATTCTCTT

CAATTGTGGG

GCGCTAGTT

TGGTTCACGT

GCTTATTAAC

TATTGGAAAC

CTIGTAGGACA

ATGAAA'PrTA

GAGGAATGAG

CGCCAGGITC

CTCAACGAG

CAGTCACACC

CCATGGGGGC

CTI'ACCAGTC

AATTGAAACA

TTTTAGAA.AT

AGCAATCTTC

TTGTTTTTTT

GGCAACAAC

TCTT'TAC

TAGATCTAAC

AAGAATGGCT

ACATAGAGAG

ACATAAGTTT

GATTATCCCA

TACCACTGGG

TTTACCGAI'

TGTTGCTGCT

TGCTATGTTT

ACCGAAAGTT

AGGACTGCTG

?I'GGCTGTAT

TI'CTTIAGAG

GCCTCTAGGT

AAACCCTGGA

ACGAAAAACA

I-rI-CCG=T

TGGTACGCTT

ATTGGTrGCG 7TI'rGTTAGC

AATTGAACCA

TAACTATCAG

TAGTTTGCTA

CGTTCATCAG

GTITAGCACAA

TAAATCCTAT

ACTTGG'-ITCA

ATATGCCGAG

GGCGAAACAA

CAAGGAGACA

GCAACAAAAT

GGAAGGAGGA

CGAGGGACAA

GTGAAAGTAA

AATTGAGTA

AATCTACGGC

CTAATTrGGTI'

ATTGCGACAG

ATTGGCCCTT

AAAGAAGGCT

TG.^TGT~rTG CAArTTTr

CCCTTAAAAG

TTAGAAGGTG

CCACGCGTOG

GCAGGAGGTG

GTTTTGATG

GTTTTTCAAA

ATTTTAGCCA

TTTCTGTGCT

GTTTCAATGA

CAAATTFrAT

AGCCGACAAT

ATGAAGTGC

AAGTACGTCC

TACCCGCAAG

GAGTCTACTG

AATTCGAG

GGAGTCGCCA

ACCTACGTAG

TTATACGTAG

GCTCATAGAT

GGAATATGAA

CTCCCTATTT

TTTTrTATTGC

TAGCTCTTTC

TTACAGGCGG

TGGGCGTGAT

TAGCAGGATA

ACGAGATGCT

GTATTCTGGT

CTTGGTTAAT

TGTTATTTT

CTAGTCAA-AC

GCGTTI"'IGGT

CCACCATGAT

ACCCGCGCIT

TATTTAATGT

ATGCCCCGAC

C ITTTGCAAT

TAAAGATGAA

TTGTTTGTGA

TAA.AAGCTGT

AGCCTAAGAC

CAGAAAAGGA

1I'TAGCCAA

AAACGAACTC

GATCGCAAAC

TTGAAGTATC

TGACTCGTGA

TTACTAGTTT

GTTTrrAA

GGAAAAAGAA

ACGTCGITPI

GTGGAGCTTA

TGAAGTCGAA

AAAAATGTTC

TGT'ITCCACA

TACI'TCGCC

GACTAAAAAC

TGTAGCTCCG

TGAAGCCAAT

TAATAATGCG

AGGTCAGTCC

TGCTTCTG

TCAACTGATT

ATTTTAGCA

GGGTC'rAAGT

TG-ATGCGAGG

AGCAAGCTTG

GGAGGAAGAC

Tw-CAGGAATG

GAACTTGGAG

ATTAGTGGTC

TATGGTGAGT

ACTGACTG-CT

GACAAAACAA

AATGGGAATG

TAAACAGCCA

ACTGGCGCAA

TTTGAATAAG

a.

192 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

EF097-2 (SEQ ID NQ:370) MLTKNF LAGIVGGLLC CFGILVVAPA

VESASFWLYQ

LFLLETNPGP

IACGMSGTLV

LKRQRGIEPV

PVTYQSVHQM

SQEQSSLGSE

ETVFFTKETT

FSSWL IEANL

GVGVLJVAFLL

FQ IFNVGLSA

SMIKMCEEDQ

KWQPKTLVVI

STETNSTKQI

YVVTRELAQA

LPLVHVFLEP

FGPVGQRK(TA

PASPGSLVAI

VEWVPNYQQ

QAELKQLAQK

QKLVFLYAEN

YHLDLTQQN'L

LKVLFFNNAI

GGATMIQLIG

LANAPTDARL

ILFVCDAGMG

YVPEKDMVSV

VRCSQTMGME

YVVTSFLNKK

N}IGLLTPLC'L

G IHE IYFPFV

AVFSCIFVSF

S SANGASLLS

QNFLEIKSYY

LLRQQAAKQG

EYQEWLEGGA

EGASQTGQS I

LMDPRLFLAV

LCSFAIASLL

RQLKAXTNLEM

PQVLAXIJTAS

VAIEVSKEPL

DRCF

EF097-3 (SEQ ID NO:371) ACGAGG AATTGAACCA GTTTCAATGA TAAAGATGAA GGAGGAAGAC

CAAGTGGAAA

GGCTCAAGTG

ATGCCTGTGA

ATTCAAGCAG

CTTCAAAATT

TCTTCTCAAG

ATACAGAAGC

GAATTATTGC

CTGGA-AACAG

GCCTATCATT

AAAGAGTATC

CAGTCACACC

CCATGGGGC

CTTACCAGTC

AATTGAAACA

TTrAGAAAT

AGCAATCTTC

TTGTTTTTTT

CGCAACAAGC

TCTTTTTTAC

TAGATCTAAC

AAGAATCCCT

TAACTATCAG

TAGTTTCCTA

CGTTCATCAG

GTTAGCACAA

TAAATCCTAT

ACTTGGTTCA

ATATGCCGAG

CGCGAA-ACAA

CAAGGAGACA

GCAACAAAAT

GGAAGGAGGA

CAAATTT'AT

AGCCGACAAT

ATGAAGTGGC

AAGTACGTCC

TACCCGCAAG

GAGTCTACTG

AATGTTCGAG

GGAGTCGCGA

ACCTACGTAG

TTATACGTAG

GCTGATAGAT

TTG3TTGTr.A

TAAAAGCTT

AGCCTA-AGAC

CAGAAAAGGA

ITTAGCCAA

AAACGAACTC

GATCGCAAAC

TTAAGTATC

TGACTCGTGA

TTACTAGITT

GTITT

TGCAGGAATG

GAACTTCGAG

ATTAGTGGTC

TATGGTGAGT

ACTGACTGCT

GACA.AAACAA

A.ATGGGAATG

TAA.AGAGCCA

ACTr.GCGCAA

TTTGAATAAG

q.

EF'097-4 (SEQ ID NO:372) RGIEPV SMIKMKlEEDQ VETVTPNYQQ ILFVCDAGMG SSAMGASLLS RQLKAVNLEM

PVTYQSVHQM

SQEQSSLGSE

ETVFFTKETT

KWQPKTLVVI

STETNSTKQ I

YVVTRELAQA

QAELKQLAQK

QKLVFLYAEN

YHLDLTQQNL

YVPEKDMVSV

VRGSQTMGME

YVVTSFLNKK

QNFLEIKSYY

LLRQQAAKQG

EYQEWLEGGA

PQVLAKLTAS

VAIEVSKEPL

DRCF

EF098-1 (SEQ ID NO:373) p p

TAAATGAAAA

CTTGTACCAA

CAATI'TGCAC

AAACCAATTA

CCCTTATCCA

AATATGACCT

GGCCCAAACT

GTAAAACAAAi

ACTI'AACCA

GCTACGATTG

GAAGGTGCGG

ATTTCATAG

ACTTGGACTT

AGACAAAAGT

TGAGTGCATI'

CAAATACGAA

CACCAG273A

TTIGACTATGC

ACTATGCAGA

ITGTTCAAGT

ATGGTCAAT

ACGGACGCGT

AATTrAAACCC GTACGTAC T

AAGTACCTGG

TGACAGATAC

ATACATTG

AGCAGTCGAC

CCCAACGAAT

TCCAACTGAT

ATCTAGCTITA

AACACAAAAA

CTCAGATAAT

CAAAACTGAA

GCTTTCAGCT

AACTCGCCT

AATGCTGG

TTCAACCACA

ACCTGCTAAC

ATGGCAACCA CAACTTTAGG GGTGGT'GAAT ACCAAACAAA CCACTTGATC CGACGAATCC CCGACAGOC CTAAGCCAGG TCTITTGGGG AACAAACGAT TACAAAGATA ACGCTGGTGC CGTCOGACTG AGACAGCTTG CCCAACCAAG AACTAACAC TCACAATCTG CAA.AGCCAAC GAATCAGTCG TCATGGCTGC GGCGATAGTG TAGATACCGC AAATATGCGA AAAAATACAC ACACCAAACT AA

CGCACTGGCA

CGGAGCGATT

AGACCCAGAT

GACAGCAGGT

TACCTCAAAA

CGACCAAGAA

GACGCTAAAA

GGCCAAAGTA

GACAGCGCCA

TGGCGATAAA

TAAAACAAGT

GAcAcTTT EF098-2 (SEQ ID NO:374) M4KKTKVMTLM ATTTLGALAL VPMSALAVDG GEYQTNGAIQ FAPNTNPTNP VDPTNPDPDK PITPVDPTDP TGPKPGTAGP LSIDYASSLS FGEQTITSKN MTYYAE'TQKY KDNAGADQEG 193 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

PNFVQVSDNR GTETGWTLKV KQNGQFKTE-A NQELTAkAKVT LSNGRVVSAS QSAKPTTAPA TIELNPTGAE SVVMAAGDKE GAGTYLMSWG DSVDTAKTSI SLEVPGSTTK YAKKYTTTFT WTLTDTPANT CN EF098-3 (SEQ ID NO;375) AGTCGAC GOTGGTGAAT ACCAAACAAA CCGAGCGATT

CA-A'TGCAC

AA.ACCAATTA

CCGTTATCCA

AATATGACCT

GGCCCA.AACT

GTAAAACAAA

ACTTTAAGCA

GCTACGATTIG

GAAGCTGCCC

ATTTCATTAG

ACI'GGACTT

CAAATACGAA

CACCACTTGA

7TGACTATC

ACTATGCAGA

ITTGT1CAAGT

ATGCTCAATT

ACGGACGCGT

AATTAAAC CC

CTACGTACTT

AAGTACCTGG

TGACAGATAC

CCCAACGAAT

TCCAACTGAT

ATCTAGCTTA

AACACAAAAA

CTCAGATAAT

CAAAACTCA.A

GGTTTCAGCT

AACTGGGC-CT

AATGAGCTGG

TTCAACCACA

ACCTGCTAAC

CCAGTTGATC

CCGACAGGGC

TCI=TGGG

TACAAAGATA

CGTGGGACTG

GCCAACCAAG

TCACAATCTG

GAATCAGTGG

GGCGATAGTG

AAATATGCGA

ACAGGAAACT

CGACGAATCC

CTAACCCAGG

AACAAACGAT

ACGCTGGTGC

AGACA*GTTG

AACTAACAGC

CAAAGCCAAC

TCATGGCTGC

TAGATACCCC

AAA-AATACAC

AGACCCAGAT

GACAGCAGGT

TACCTCAAAA

CGACCAAGAA

GACGCTAAAA

GGCCAA.AGTA

GACAGCGCCA

TGGCGATAAA

TAAA.ACAAGT

GACAACTF1 EF098-4 (SEQ ID NO:376) \TDC G2YQTNGAIQ FAPNTNPTNP VDPTNPDPDK PITPVDPTDP TGPKPGTAGP LSIDYASSLS FGEQTITSKN MTYYAETQKY KDNAGADQEG PNFVQVSDNR GTETGWTLKV KQNGQFKTEA NQELTAAKVT LSNGRVrVSAS QSAKPTTAPA TIELNPTGAE SVVMAAGDKE GAGTYLNSWG DSVDTAKTSI SLEVPGSTTK YAXKYTTTFT WTLTDTPANT GN EF099-1 (SEQ ID NO:377) C

C.

C

TGATGTTGTA

ATGAAGAAAT

TTTTAG

ACACCACAGG

TTGCAGTT'G

AGAATTCAAA

TATGGAAITG

AA.AAGTCAAA

GATGCTTCTG

GCGGTAGATA

GTAAA.AAGAA

GGAGGTTCAG

ACTATTAATG

CTACAAATGA

AGTTCAI'TG

GTCATTAAIT

GCAAGTGTTG

ATTGTTGCTA

Ar-GAAATAG

GAGGGCTGAT

TAGGCAAGGT

GTGTAITTC

AA.AAAGTAGC

CTTCCGCTTG

GTGAT=ATC

GGTTAGGACA

AAAAGGAATG

ATAGTGACTT

TITGAAGCT

AGGCTAGTGC

CCAATGTTGG

GTGGTCAATG

TGGGTACGGG

GTTGGACAGT

TGTCAAGG

AAGGTAAAAA

AGTATTTITCG

GAAATGTTTA

TTAALTGTT

TTCTAGTGAA

ATI'AGAAGTT

GATTGGCAAT

GTTTAATTCA

ATGGGATTCA

GAAATCAGTA

ACTTAAAAGA

GTGGGAACGA

TAATAATI!GG

TGGGAAAA

TTATGCTA

GCATAM='1

CATAAAGAAT

TGGTGTGGCT

CAAGT'ITACT

GACTI'GGGGA

TCAGTCTTCT

AGTTGT=1A

ACCGGACATT

TCTAACTACG

ATGGAACATG

GCGATAGCTT

GGACGA-AGAG

GCTTTACAAA

ATGTCTAAAT

GCTGGAACA.A

TATAA.ACGAC

ATTGATCCCT

TCTGC PI=

GCGAGTCAAA

CCAAATTATT

ACTAGTATT

ACTTATGAGC

TTAGATTC

TTTATTGAA

TTTTTATTCT

CTTCCCAGTT

TGACGT-CACA

AAAGTGGATT

TTAkATCCTTC

TTAATTTATT

TGGATCTTC

CAA.AAGATCT

AAGATGATCC

TTCTACAGG

TGGAAAAAGT

TTTGAAAA

TTGGTAATGA

CAGATATTAA

ATGGGCATAC

AAA.ACGCTGA

CACATGTGAC

AGGAGAGATC

TCCTTTA

TCAGCCCGCT

TGGCGGAACG

AAATCCTGCT

GTTAGGCGGT

AAATTTTGCA

GTGGAATP.AG

GAATACACI'

CGCAGAACAA

TTCCATGGGC

GATGGGGCAA

ACAAGGACCGT

'ITATCCTTGG

AGCAGGAGAT

TGGTGTAGTG

ACAAGGTCAA

CAGCATAGTA

C

C C C C CC EF099-2 (SEQ ID NO:378) MKCLS VFFL.LKGEIM KKLGKVLIVS CFIFILPFLL FLGVFSSSES GDSSQFQPAT PQEKVALEVS NYVTSHGGTL QFASAWIGNN EHESGLNPAR IQSDLSFNSA IAFNPSLGGY GIGLGQWDSG RRVNLLNFAX SQKKEWKSVA LQMDFAWNKD GSDSDLLKRH SKSKDVNTLA 194 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

VDILKLWERA GTKDDPAEQV KRKASAi\TNWY KRLSTGSMGG INGGQCYGLS AFFVEXQGCL QMMGTGHMFA SEIGNDYPWS I Z'FGQGG VAT S IYGHTGVVA SVEGKNKFTT YEQNAEQGQ I

K

E.7099-3 (SEQ ID NO:379) TAGTGA.A. AGCGAGATT CTTCCCAGTT TCAGCCCGCT

OSANVGGGKI

SIrGWTVIKNP

VA-KYFRTWGL

CALEKVMGQT

NYSDIKAGDV

CFPHVTS IVR

ACACCACAGG

TTGCAGT='

AGAATTCAAA

TATGGAATTG

AAAAGTCAAA

CATCGTTCTG

GCGGTAGATA

CTAAAAAGAA

GGAGGTTCAG

ACTATTA-ATC

CTACAAATCA

AGTTCAATTG

GTCATTAATT

GCAAGTGTTG

ATTGTTGCTA

AGGAAAT

AAAAAGTAGC

CTTCCGCTTG

GTCATTTATC

GGTTAGGACA

AAAAGGAATG

ATAGTGACTT

=ITGAAGCT

AGGCTAGTGC

CCAATGTTGG

GTGGTCAATG

TGGGTACGGG

G TGrGACAGT

TTGGTCAAGG

AAGGTAAAAA

ACTATTTTCG

ATTAGAAGTT

GATTOGCAAT

GTTTIAATTCA

ATGGGA'FrCA

GAAATCAGTA

ACTTAAAAGA

GTGGGAACGA

TAATA-ATTGG

TGGACGAAAA

TT'ATGGCTI'A

GCATATGTTI

CATAAAGAAT

TGGTGTGGCT

CAAGTTTACT

GACTTGGGGA

TCTAACTACG

ATGGAACATG

GCGATAGCTT

GGCGAAGAG

GCTTTACAAA

ATCTCTAAAT

GCTGGAACAA

TATAAACGAC

ATTGATGCCT

TCTrGC=T

GCGAGTGAAA

CCAAATrATT

ACTAGTATTT

ACTTATGAGC

ETAGA'IWC

TGACGTCACA

AAAGTGGATT

TTAATCCTTC

TTAATTTATT

TGGATTTTGC

CAAAAGATGT

AAGATCATCC

TTTCTACAGG

TGGAAAAAGT

TITCTTrAAAA

TTGTAATGA

CAGATATTAA

TGGCCGAACC

AAATCCTGCT

GTTIAGGCGGT

AAATIPrGCA

GTGGAATAAG

GAATACACI'

CCCAGAACAA

TTICCATGGGC

GATGGGGCAA

ACAAGGAGGT

TTATCCTTGG

ACCAGGAGAT

ATGCGCATAC TGGTGTACTG AAAACGCTGA ACAAGGTCAA CACATGTGAC CAGCATAGTA EF099-4 (SEQ ID NO:380) 9 0.

0* 0 9 9.

S

9 SES GDSSQFQPAT PQEKVALEVS NYVTSHGGTL GIGLGQWDSG RRVNLLNFAK VDILKLWERA GTKDDPAEQV INGGQCYGLS AFFVE-KQCGL INFGQCGVAT S IYGHTGVVA

K

QFASAWIGNM

SQKKEWKSVA

KRKASANNWY

QMMGTGHMFA

SVEGKNKFTT

EHESGLNPAR

LQMDFAWNKD

KRLSTGSMGG

SEIGNDYPWS

YEQNAEQGQ I

IQSDLSFNSA

GSD)S:LLKRM

GSA.NLVGGGK I S IGWIVIKNP

VAXY.TRTWGL

IAFNPSLGGY

SKSKDVNTLA

DALEKVMGQT

NYSD IKAGDV

DFPHVTSIVR

EF100-1. (SEQ ID NO:381) 0

TANTTATGGC

ACATI'ATTAG

CCTAACTTAG

ATTGTAGAAT

GAATTACTCA

CAATGA

AATATGGAAG

AAATGTTGAT

CGAAACATAA

CACAAATCGA

ACGAAGGCTA

GAGTTTTATA

TGTCTTATTG

AGAAACAGTT

GCTCTACACA

CATTACTAAA

ATGAAP.AAGA

ATATr~rCG

GATAAAAAAG

CTAGAAAAAA

GAGCAGTI'AG

AACAAAAATA

TAITATTI'

GCAAT--AAGC

ATAAGACGCC

ATAAATATAC

CGCAGGGCTI

ACTTT'ITGTC

AATCGTAAA.A

TTCCTTA-AAT

AGCAGAAAAG

EFI00-2 (SEQ ID NO:382) MKKKQKYAGF TLLENL IVLL II SVL ILLFV PNLAKHXEfrV DKXGiqEAIV'K IVESQIELYT LEKNXTPSLN ELVNEGYITI( EQLDKYTAEK Q EFIQO-3 (SEQ ID NO:383) TAA AGAAACAC'IT GATAAAAAAG GCAATGAAGC AATCCGTAAAA ATTGTAGAAT CACAAATCGA GCTCTACACA CTAGAAAAAA ATAAGACCCC TTCCTTAAAT GAATTAGTCA ACGAAGGCTA CATTACTAAA GAGCAGTTAG ATAAATATAC AGCAGAAAAG 195 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes,

CAAT

EF100-4 (SEQ ID NO:384) KETV DKKGNEAIVK IVESQIELYT LEKNKTPSLN ELVNEGYITK EQLDKYTAEK Q EF100-l (SEQ ID NO:385)

TANTT~ATGGC

ACATI'ATTAG

CCTAACTTAG

ATTGTACAAT

GAATTAGTCA

CAATGA

AATATGGAAG

AAATGTTGAT

CGAAACATAA

CACAAATCGA

ACGAAGGCTA

GAGTTTTATA

TGTCTTATTG

AGAAACAGTT

GCTCTACACA

CATTACTAAA

ATGAAAAAGA

ATI'ATTCCG

GATAAAAAAG

CTAGAAAAAA

GACCAGTTAG

AACAAAAATA

TATTGATTT

GCAATGA.AGC

ATAAGACGCC

ATAAAT

1

ATAC

CGCAGGGIT

ACTTTTTGTC

AATCGTAAPA

TTCCTTAAAT

AGCAGAAAAG

EF100-2 (SEQ ID NO:386) MKKKQKYACF TLLEMLIVLL IISVLILLFV PNLAKHR!ETV DKKGNEAIVK IVESQIELYT LEKNKTPSLN ELVNECYITK EQLDKYTAEK Q EF100-3 (SEQ ID NO:387) TAA AGAAACAGTT GATAAAAAAG GCAATGAAGC ALATCGTAAAA ATTGrTAGAAT CACAAATCGA GCTCTACACA CTAGAAAAA.A ATA.AGACGCC TTCCrrAAAT GAATTAGTCA ACGAAGGCTA CATTACTAA-A GAGCAGTTAG ATAAATATAC AGCAGAAAAG

CAAT

EF100-4 (SEQ ID NO:388) 4.

S.

a KETV DKKGNEAIVK IVESQIELYT LEKNKTPSLN ELVNEGYITK EQLDKYTAEK Q EF101-1 (SEQ ID NO:389)

S

5* TGAGGAGATG AAACGAAGAA GTAATTGCGG TTGGGGGCAT GCTGTCAAGC AAGCGCCTAA CAACAAATTT ATArI'AACGG CAAAAAATAA CAAAGGATCC ACACAATTAT ITACTI'ATGA AGCTTACCCA AATTAGAAAC GATAAATTTA ATAAAACTAA CAATATCAAA CAGAAGTCGA GCGGATI'TAG GAGCGAAGCA ATTCCAGAAG TAAAAGATGC TTAGCTGGAA AAGTGAATGA CTAACTTCTG TTTCCAACAA CCTCCTGAAG GCAACAGCGA AGTTATAGCG TCAAAATTGC CAAGCAACCA TrrGAT1'TAGG AAAGAGGGTG AACAGG-CCTA GTCCAAGTCG GGCAAGAAAA GACCCAGTGG TTAmTCTTC

CGTAACTGTG

AGATGACTGG

TGTCATCCAA

AGAGATTAAG

AGATGAGGCG

GAAGCGGGCG

AGAAGAAGAC

TGCAGTAGAT

ATATATTTCC

CAATTCACCG

AAAGGACTTG

TGTGGITGTG

TGCCGCGAGT

GTTGGCCAAT

CGATTTAGGG

CGTTTTAGG

TGGCGACAAA

AAAAAAACCA

AATGCGTTAA

GGAATTrGACT

CCCGAACAAA

GTGAA-AAACG

GTCACAAAAG

AATATCTATA

CGCACGATGT

GAAGAGXITA

ACAAAGG3CTA

AT=TACGGT

ACTAAAATTA

GATGGCTCAA

GGCAATCCAG

TTAGACAAAG

GCGATTGAGT

AATGA=~G

ATGGCGATTG

GTAAAAGTCG

AAlrGAAGAAG AAAACGATAA TTATATTGGG GGCAGTTGCG ATAAAAATGC ACAACAAGTA ATI'TTGACGT TCCCGACITG TGGAAGCCTT TGCGCGTGAT GCGATGTCGT AGATOGCAGOC AAATTGAGGC ACAACAAAAT ATAAGTGGAA TCGGGCCATT CTGGTGATGA TTTAAATGA-A CCTTCACCAA TGAAACCTTA ATTTCAAAGG TCGTGTATCA TAACTTCAGA AGATCFI'TAT GTGT IGGGCA AAAAGCTAAA TTTCTTACAT CGATGATAAT AGGGCGGCAC AACGATGTCT TCAAAAATGG CTACCATATG TACCGAAAAA AGCGATTCAA GAACCATCAT TCGTCGTGAT AATCMGCTT AGAATCAGCC GTGATATIGT TGAATCAGAT 196 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

GCAGCGATTG CTCTATGA ATCAGCAACC AACGAATCA-A TGACAGATGC GTCGAAATAG EF101-2 (SEQ ID NO:390) MKKK TIIILGAVAV IAVGGIVTVN ALNKNAQQVA VKQAPKDDWG IDYFDVPDLQ

QIYINGVIQP

LA.KLETKRAN

DLGAKQYIST

TSVSNNVWVD

ATIDLGDLGA

R'JVISSKKPV

EQMEAFARDQ

IYNKWNRAID

KANFXGRVS I

GSISYIDDNP

IELPKKAIQK

KVGDIVESDA

KITKDPE 1KV

KFNKTKEEDR

P EVKDANS PI

PEGNSOAASG

EGEQAYVLVN

AIASDESATN

KNGDVVDAGT

TMSGDDLNEQ

LRLTSEDLYL

NPEGGTTMSS

OFGTI IRRDV

ESMTDASK

ELFIYEDEAV

YQTEVDAVDE

AGKVNE-KOLT

YSVKIALANL

QVGQENGDKI

TKEI EAQQNS EITFTNrTLA

KISVGQKAKL

DKVKNGYHMQ

AIESGLESAD

EFIO1-3 (SEQ ID NO:391) TAAAAATGC ACAACAAGTA

GCTGTCAAGC

CAACAAATTT

CAAAAAATAA

ACAGAATTAT

ACrAGCCA

GATAAATTTA

CAATATCAAA

G'CGGATTTAG

ATTCCAGAAG

TTAGCTGGAA

CTAACTTCTG

CCTCCTGAAG

AGTTATAGCG

CAAGCAACCA

AAAGAGGGTG

GTCCAAGTCG

GACCGAGTGG

GCAGCGATTG

AAGCGCCTAA

ATATAACGG

CAA.AGGATCC

TTACTTATCA

AATTAGAAkAC

ATAA-AACTAA

CAGAAGTCGA

GAGCGAAGCA

TAAAAGATGC

AAGTGAATGA

TTTrCCAACAA

GCAACAGCGA

TCAAAATTGC

T'TGATTTAGG

AACAGGCCTA

GGCAAGAAAA

TTATTTCTTC

CTTCTCATGA

AGATGACTGG

TGTCATCCAA

AGAGATTAAG

AGATGAGGCG

GAAGCGGGCG

AGAAGAAGAC

TGCAGTAGAT

ATATATTTCC

CAAITCACCG

AAAGGACTTG

TGTGGTTGTG

TGCCGCGAGT

GTTGGCCAAT

CCATTTAGGG

CGTTTTAGTG

TGGCGACAAA

AAAAAAACCA

ATCAGCAACC

GGAATTGACT

CCGGAACAAA

GTGAAAAACG

GTCACAAAAG

AATATCTATA

CGCACGATGT

GAAGAGATTA

AC AAAGGC TA

ATTTTACGGT

ACTAAAATTA

CAT'GGCTCAA

GGCAATCCAG

TTAGACAAAG

GCGATTGACT

AATGATTTTG

ATGGCGATTG

GTAAAAGTCG

AACGAATCAA

ATTTTOACGT

TGGAAGCCTT

GCGATGTCGT

AAA'ITGAGGC

ATA-AGTCGAA

CTGGTGATGA

CCTTCACCAA

ATTTCAAAGG

TAACTTCAGA

GTGTTGGGCA

TTCTTACAT

AGGGCGGCAC

TCAAAAATGG

TACCGAAAA.A

GAACCATCAT

AATCTGGCIT

GTGAkTATTGT

TGACAGATGC

TCCCGACTTG

TGCCTGAT

AGATGCAGGC

ACAACAAAAT

TCGGGCCATT

TTTAAATGAA

TGAAACCTTA

TCGTGTATCA

AGATCTTTAT

AAAAGCTAAA

CGATGATAAT

AACGATGTCT

CTACCATATG

AGC-GATTCAA

TCOTCGTGAT

AGAATCAGCC

TGAATCAGAT

GTCGAA.AT

EF1OI-4 (SEQ ID NO:392) KNAQQVA VKQAPKD)DWG IDYFDVPDLQ QIYINGVIQP EQMEAFARDQ KITKDPEIKV LAKLETKRAN~ IYNKWNRMID KFNKTKEEDR DLGAKQYIST KAN~F'KGRVSI PEVXDANSPI TSVSNNVVVD GSISYIDDNP PEGNSDAASG ATIDLGDLOA IELPKKAIQK EGEQAYVLVN RVVISSKKPV KVGDIVE-SDA AIASDESATN EF102-1 (SEQ ID NO:393) TAAACAT=r

ATTAATTATA

TT1AACTCAAA

CACCCTCAAG

TTTAGCGATG

GCAAAACAAA

GGITATCAGA

TGGGAAAAGG

CAACTGAACG

AGACATTCAG

TGAAAAAAAC

CAA ETGGCGG

AGGTAACAAT

GCAGCACGCT

CAGTATTTTG

AAAACCCTTT

CTGGAACAGA

GTTATAAACT

AGGTGAATGT

AACATTI'AAA

AACGATTGGT

TCATTATGAC

CTCAGAACGT

TATTGAACCA

GCCATCAATG

TATTGATACA

CCATTCCATA

KNGDVVDAGT

TMSGDDLNEQ

LRLTSEDLYL

NPEGGTTMSS

DFGTIIRRDV

ESMTDASK

CTC'I=rTTA

AATTGTCGT

CCTACGATTG

GTAAGTAAAC

ACTACGTCAT

GGTGTTAGTA

TCTGATAAAG

AATATGG=

AAAAGATTAG

ELFTYEDE-AV

YQTEVDAVDE

AGKVNEKDLT

YSVKIALANL

QVGQENGDKM

TKEIEAQQNS

EITFTNETLA

KISVGQKAKL

DKVKNGYHMQ

AIESGLESAD

TACTCAAAA

TAITTOrCGAC

C'ITIGCCGA

TGTATGAAGT

TATATGCAGA

'FTCCAACAGA

CGAAACTAGT

CACAAAACAT

GTGGTGC'

ACGAAAGGGG

TITGGCTCTA

TGAAATTACT

TGACGGAACT

ATACAATGGT

AGTGACGCAC

ATCGGTTCTT

GATTTGGGAA

AGTTGATATA

197 TAB3LE 1. Nucleotide and Amino Acid Seqeuences; of E. faecalis, Genes.

AAATCTATTG

CATAATACCA

TTAAA='AT

ATTGGGAATC

AAAAAATCAG

ATGGCTGGTG

GGTTCTTTAA

TTCCATTTAG

ATTTCTAIT

CCAGATCCTT

ATTTCCATGA

GTAGAAACAG

ATTCGTAAAG

CGTGCGGAAA

GAAACTAAAT

TATGCCAATC

ACTGGGGAAA

GCTGAGTTTA

TGGAGTGAAG

ACTTTGCCTT

TTCTGGCAAG

TCCATCAAAA

AA.AGAACA-AG

ACTGCCGAAA

GAAATCACAG

GGCTATGGTA

CCAGAAGGAT

GACGACTATG

ATTAAGATG

AACCGTTTGA

TGGAAAGACG

AGATATAACT

GAAGCCACAA

ACAACCGCAA

ACCGCTGAAC

GTAGCCT'IG

CAAACCGTCA

GATGGTTCGC

ACCCATCATG

CCAGATGGTA

AAAGAAIM1A

ACTAAGTTA

AATGAAGATT

AGTACGCCAA

ACAGTGAACA

TrTATCTrGCA

AAGGAAAAAT

CTGTAAAAAC

CTGAG'ITGA

AATTAGTGCT

CTGGTACTGG

CGCTTGATAA

AGATCAAAAA

ATTT-GGGAA

TGGATGGAAT

ATATCATTGA

CTTCGAAAAA

GTACTGATCT

ACAGCCCAGC

CACCAAAAGA

CTAGTAATGG

AAACCGTGGC

CCAC'ITGAC

AAGGAGCTGA

C=~TAAAAC

TAGATGAAAA

AAACCAAAGC

AAGTTGATALA

TTATTCCCTT

CTGGATTIALA

GTGCTGAAGA

AG'ITTGAAAA

TTCAAAAGAT

CGAAGAGTGA

TCACCGTTCC

TGCTTTATGA

GAAATAAAAA

TGCATGAAAT

AAGCTC-CCCA

CGTTGGCGAA

AAGTTTTGGA

AAGCAGGCAA

ATTGTACGAT

AAACTTTTAC

TACTGGATCC

CGAACAAAGA

CCAAAACCGT

CTTTACGGA

TGAAAGAAAA

AACAACCGGA

CATTCCCGCA

T'TCGAC

TAATAAGGCA

AATTTTIAGGT

TAAAGTCGTC

TACTCCAAAC

AACTCCAGTC

GCCCAATACC

AATCGATAAA

ACCTTTACCT

TCCCCATOGT

TACCACACCC

TATGCGACAA

TTGGA.ATGAC

TGGTGAA.ATT

GCTTGCTAAT

'TTCGTGAAT

TCTI'GTTACC

AAAAGAAGAC

ATATACTCTC

AGAATTAGTG

CAACCAACTT

ACCTGAAGGA

TAACGAAAAA

TGCTIGAT

CGACTTATCT

TA.AAGCGACC

TC~rCCITAT

TACACCACTA

GTATGTCTTT

LTACGAGAAA

TTTGCCCGAG

ATTGAATACC

CAAAGAAGAC

AGAAAAAGAC

CAAAGAGAAA

TAAAAGCATC

TGAGCCAGTA

TGAACGCCAT

TCATGGTGAC

CTCAAAAGAA

AATTTGGAAA

ACTTGCGGAA

AATCAATTAC

ATCTCAAACC

AACACCAGCT

ATTGAGGAGT

CAATCGACAA

CAAAATACGG

TCTA.ATrCCA.

GCTTATAAAA.

TACGCTATTA

GAATCAGGTC

TCAAAAGATC

ACAAAGGTAA

ATCCCTGCCA

AAAGGTCAA.A

AATT'ATrCTC

GTCCAAGAAA

GCTTTGAAC

ACCTTCAAAC

AGTA.ACGTAA

AAAGATACCG

TrACTGCAA

AAGGGAACGA

GCCGTTAAAC

TATACT7MG

AATGCCGTAA

TTCTTTAAAT

TTAAAGTGT

ACAGCTTGTA

GGGGATTATT

GAAATCCGTT

ACCATTACC.G

CTAACTAACA

AAAGAAGATA

CTTGA=TTA

TGGTATGTCG

GAAAAAGCCA

ACTGGAACTT

GTCI'CTTCA

GCGAAGGATC

GTTTCCATCC

GTGATGGATA

GCTTTCGAAA

TCTGrGCAAAA

AAAGTAGATA

GAAAAAGATG

TTAACACCAA

GTTCCAAGTA

PAICAAAAAA ACCA.AGTT'T CTTTAATAGA TAAAAATGAA cGAATATAGA rTACCGTGTA AATCAGGA-AC AFI'AACATTG A.AGCAGGACT TCAAACTGTG AAA~rAATGT GGAAACTAAG ATATTCTACC AGAAACGGTT TC3ACXACAGA TAMA.GATGGG CTATTACTGA AAAATCGGTG CCATTAAAGC GGGCGAGACC TTCTTTTAGA GAAGACTGGG TAGCTGGAAA TACATTIGCC TA.ACA.ACGGA TGAAAAAGGT TGGGAA~CCTA TTACGTGACA CAACAAAAGT CGAGTTAAAA AAGGGCAAAA CCAAGAAATT GTAATGAGAG TCAAGGGAAA AAGATCGTCA AGCTGTTAAA AkAGCTTCTG.A TGAAACAGTG ACCTAGCAAT TAACGAGTAT ATGAALACGAA GTATCCTGTA TTACTCGAGA TGTTACGGCA TTCCTGGATC GGCTGATGGC CGCCATTGGA AGGC-ACCAAN ACGAGCAALT AGGTTTTGAT TACTTGAAGA AATAGAGGCT CTACATT'AA GGAAAACAAA AAGAAGGACA AAAACAACCA ACGAGTI=C TGTTAGTCTG CTTTAC1'IC TCTTGCGACT CCGAGCTAGT TGATAAAT'G TAGCTCAAGC CATTGATGTG A.ACCGGTGGT GA TCGCCGAA GGAAAATrCT GCATAAATTA ATTATGTGTA TGAAAACAAG CTAGCTTGAA cAATCAAGCA AAACAAAAGC CCACCTAGAA TGT'IGATGA TGTGTCGGT' CAA7MCTGTA TGCTTI'ACTA TTCACCATCGA AGTGAATGAT CCGGAAAGTA TCCAGAAGGA GAAACGTGAA TGGAAAACAC A.AGAAGTGCC AACCATACCG AT1rCTCAAGA ATCTAGTCCC ACGTTCTACT GTTAGITGGC ATCGCCGCAA TTAA

S

S S 555 5

S

S

S 5555 S C AACTGGGGAG .AAAAATTCCA TGCTGGGTAT TATTTCTGGA EF102-2 (SEQ 10 NO:394) MXKTTFKN WSLFATLA.L SQTIGGTIGP TIAE'ADEITH PQEVTIHYDV SKLYEVDGTF SDGSTLSERT TSLYAEYNGA YQKNPLPSMS DKAKLVSVLW EXAGTDIDTN MVAQKIIWEE SIEGKINXAI EEYQKKPSFH NTTVKTILGQ STTLIDKNEL GNQLVLTPNS NSKSGTLTLK KSAGTGTPVA YKKAGLQTVM KQTVFC IEPG VNGYKLHS 1K

NLSEFDKVVQ

AGALDKPNTY

VSIPTEVTHG

RLGGASVDIYK

NTANIDYRVI

AIKINVETKG

198 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

SLKIYKKIDKE

DPYMIDTTPM

RKDS PAGE IV

ANQTVALVTS

SEAFKTELVK

IKKVDNNEKN

ITGAEDKATI

DYARSEYVFT

KDGNKKLNTL

TATLANKEKT

TVNCTIERHV

OGTNKEIWKS

EDLKEKSQTL

ILIFSTAGYY

SGDIVPETVF

AATIKAGETI

QEITTDEKGR

NVKGQNQEIT

GTKASDETVT

AV ITRDVTAK

ACNEQLGFDG

ITEEGQKQ 21

DFTELVOKLR

GTWKILHKLT

SIQTKAHLED

GK IEHEVNDK TPKEVPTI PS

FWNRRN

HLDFGKA.LPS

SMTSKNMRQK

AETPKELANA

GET'LTKEDK

L.AIDEKNQVA

EQVIRFGFDF

YGKFENLPYG

KMVTVPYEKL

YNLH-EIKEDW

AEQVLDKS IV

GSQTFTHGDV

EFTK'IVLAEK

TPKQPETPAV

KDVTTDKDG I GQ ILLEKTGV

LE-LGTYVVTE

DTGNESQGKA

VKHLAINEYF

FKFAGSADGT

DYLLEEIEAP

TN'NEFSVSLN

YVVAQAIDVE

LFNYVYENKV

MOMFDDVSVT

VDTGKYPEGT

PSNSQESSPT

SILDGIPHGT

ETGTDLWNDN

TKSSNGFVNT

EVKGAEYTLF

WQETKAPEGY

AETGFNDLSF

EGFQKITPLE

RLMLYOLPEK

ATKA.AQEKDE

AFEAGNEPVA

HDVLDGSKEA

KFTFTE INYE

VKTFPQTGEK

KVTITEKSVP

YSLAGNTFAI

FKPTKVELKY

TAKDGQAVKW

TLDETKYPVS

KVS PLEGTXE

IRSTFXENXD

EDSLTSLATW

KAKPVVIAE.T

KDASLNNQAQ

FETILYALLP

KDGNVNGKHN

NSNVLLLVGF

EF102-3 (SEQ ID NO:395) TT TAGATGAAAA GAACCAAGTT GCCG ITAAAC ACCTAGCAAT TAACGAGTAT

S

S.

S

S.

S S SSO a.

S S

S

S

TTCTGGCAAG

TCCATCAAAA

AAAGAACAAG

ACTGCCGAAA

GAAATCACAG

GGCTATGGTrA

CCAGA-AGGAT

GACGACTATC

ATTAAGATGG

AACCGT ITGA

TGGA.AAGACC

AGATATAACT

GA.AGCCACAA

ACAACCGCAA

ACCGCTGAAC

GTAGCCTTTG

CAAACCGTCA

GATGGTTCGC

ACCCATGATG

CCAGATGGTA

AAAGAATTTA

ACTAAGTTTA

AATGAAGATT

AGTACGCCAA

AAACCAAAGC

AAGTTGATAA

TTATTCGCTT

CTGGAT1'TAA

GTGCTCAAGA

AGTTTGAAA-A

TTCAAAAGAT

CGAAGAGTGA

TGACCGTTCC

TGCTTTATGA

GAAATAAAAA

TGCATGAAAT

AAGCTGCCCA

CGTTGGCGAA

AAGTTTTGGA

AAGCAGGCAA

ATTGTACGAT

AAACTTTTAC

TACTGGATGG

CGAACAAAGA

CCAAAACCGT

C TITTACGGA

TGAAAGAAAA

AACAACCGGA

ACCTGAAGGA

TAACGAAAAA

TGGCTTTGAT

CGACTTATCT

TAAAGCGACC

TCTTCCTTIAT

TACACCACTA

GTATGTCTTT

TTACGAGAAA

TTTGCCCGAG

ATTGAATACC

CAAAGAAGAC

AGAAAAAGAC

CAAAGAGAAA

TAAAAGCATC

TGAGCCAGTA

TGAACGCCAT

TCATGGTGAC

CTCAAAAGAA

AAITTGGAAA

ACITTCGGAA

AATCAATTAC

ATCTCAAACC

AACACCAGCT

TATACTITTGG

AATGCCGTAA

'TCI'TAAAT

ITTAAAGTGT

ACAGCGTA

GGGGAT'rATT

GAAATCCGTI'

ACCATTACCO

CTAACTAACA

AAAGAAGATA

CTTGAmMTA

TC;GTATGTCG

GAAAAAGCCA

ACTGGAACT

GTCTTCGITCA

GCGAAGGATG

GTTTCCATCC

GTGATGGATA

GCTTTCGAAA

TCTGGCAAAA

AAAGTAGATA

GAAAAAGATG

TTA-ACACCAA

GTTCCAACTA

ATGAAACGAA

TTACTCGAGA

TTCTGATC

CGCCAITG'-A

ACGAGCAATT

TACTTGA-AGA

CTACATTTAA

AAGAAGGACA

ACGAGTTTTC

GTTTGACTTC

CCCAGCTAGT

TAGCTCAAGC

AACCGGTGGT

GGAAAATTCT

ATTATGTGTA

CTAGC'ITGAA

AAACAA.AAGC

TGTTTGATGA

CAATTCTGTA

TTGAGCATGA

CCGCAAAGTA

GAAACGTIGAA

AAGAAGTGCC

ATTCTCAAGA

GTATCCTGTA

TGTTACGGCA

GGCTGATGGC

AGGGACCAAN

AGGTTTTGAT

AATAGAGGCT

GGAAAACAAA

AAAACAACCA

TGT'TAGTCTG

TCTTGCGACT

TCATAAATTIG

CATTGATGTG

GATTGCCGAA

GCATAAATTA

TGAAAACAAG

CAATCAAGCA

CCACCTAGAA

TGTGTCGGTT

TGCTT'rACTA

AGTGAATGAT

TCCAGAAGGA

TGGAAAACAC

AACCATACCG

ATCTAGTCCC

ACAGTGAAGA

EF102-4 (SEQ ID NO:396) LDEKNQVA VKHLAINEYF WQETKAPEGY TLDETKYPVS

IKKVDNNEKN

ITGAEDKATT

DYAKSEYVFT

KDCNKKLNTL

TATLANKEKT

TVNCTIERHV

DGTNKEIWI(s

AVITRDVTAK

ACNEQLGFDG

ITEEGQKQPI

DFTELVDKLR

GTWK ILHKLT S IQTKAHLED

GKIEHEVNDK

EQVI RFGFDF

YGKFENLPYG

KMVTVPYEKL

YNLHE IKEDW

AEQVLOKSIV

GSQTFTHGDV

EFTKTVLAEK

FKFAGSADGT

DYLLEEIEAP

TNNEFSVSLN

YVVAQAIDVE

LFNYVYENXKV

MDI4FDDVSVT

VDTGKYPEGT

A.ETGFNDLSF

EGFQKITPLE

RLMLYDLPEK

ATKAAQEKDE

AFE-AGNEPVA

HDVLDGSKEA

KFTFTEINYE

KVSPLEGTXE

IRSTFKENI<D

EDSLTSLATW

KAKPVVIAET

KDASLNNQAQ

FETILYALLP

KDGNVNGKHN

199 TABLE 1. Nuclectide and Amino Acid Seqeucnces of E. [aecalis Genes.

EDLKEKSQTL TPKEVPTIPS TPKQPETPAV PSNSQESSPT VK, EF103-1 (SEQ ID NO:397)

TAAGATAGGT

ATTACTTTAG

GATCATGCAG

CAAACAAGAG

GTTTATGATG

AAATATGATG

GGTGACGA-AC

ACACAAAATT

AAGCGTTTAG

TATCATGGGA

AAAA'ITGTGA

AAAGTCGTAG

GCGA.AA'11T0

CGCGGCGACT

ATTGGTACGA

ACGTATACTC

GAACCTTACC

?I'ATCAAAGA

TAACATTAAG

CCAATCCAAA

GCGACAAGGC

CTGCTGGTAA

GTGAAACCGG

GAACATTrTTT

ATCA.AGCCGT

GGAAAGATAA

AAAAATI'AGC

CAAATAAATC

ATAAAAACGG

ATCCAAACAC

TTGGGTACTT

ATCGTTACGG

GAATGGGTAA

AAGGCACATA

AAAGGAGCGA

TACGTTACTT

TAGTGCTACA

GACTAAGATT

TGATTAACG

TFT=IACGAG

TGTGACAGGT

AGTGGATTTA

ACTGGGGAAT

'ITIACAAAT

AGG3ATAAA

TAATGATGTG

AAGTAAATAT

CCAAGTGGTG

CGCGGCGCTA

AGATAATGCT

TCATCCAGCC

TIGCTTTATG-A

ATAGCAGGTT

CCAAA21AG

-TATCTGGCA

GCAGAAAATG

T =TTCGACA

GCATGGCACA-A

ACCGAAGTGA

GATGTTGAAG

GGACGTGAAG

ATTTTAGGAA

ACAGCGGCCA

GAATTTCA

GACAATAACA

GAATTAACGG

GGTAATGATA

AAAAGAAA'-T

ATGCAAGTCC

GCAAACATCA

CGGACTGGCA

CTAATTTTAT

AAAATACTGG

AACGAATTTT

GTAAAGACNA

TTTACGTGGA

CATTAACCAA

CA.ACCTTGTr,

ATCAAAA'IT

AKrrA^AAAC

AGATTCGGGC

AACTAAACAA

TTCAAGTGTT

A'ITAAGTTCr-

AGCATTTGCA

AAACAATGGC

AGGAACCCGT

TGGTTTAGCA

GGAAACCCGT

AATTTCCCGG

ATTTACTTAC

ACACATCCCT

TCAAACTGGC

GAATGGCACA

CATTAGTTA

AGGTGAAACC

CCATGTATCT

TGATCGATT

CGTGGAACAT

=ITACITTTCT A-A EF103-2 (SEQ ID NO:398) 9 9 *99.

.99.

9 9999 9 9 9.

99 MXKKVLSSI TLVTLSTLLI AGYASPAFAD HAANPNSATA NLGKHQNNGQ TRGDKATKIL SGTDWQGTRV YDAAGNDLTA ENANFIGLLAK YD)GETGFYEF DEGTFFVTGD GTKRILISRT QNYQAVVDLT EVSKDXFTYK RLGKDKLGND HGKKLAFTNG REALTNQTGK IVTNKSGDKI LGTTLWNGTK VVDKNGNDVT KFDPNTSKYE FFNLQTGETR GDFGYFQVVD NNKIRAHVSI GTNRYGAALE YTRMGKDNAG NDIQVFkVEHE PYQGTYMPAF TF

FDKNTGETRG

VEVY"VEH IPY AANQNFI SLA

LTELNNDRFT

EF103-3 (SEQ ID NO:399) TCATGCAG CCAATCCAAA TAGTGCTACA GCAAXI'rAG GCAAACATCA AAACAATGGC

CAAACAAGAG

GTTTATGATG

AAATATGATG

GGTGACGAAG

ACACAAAATT

AAGCGTTTAG

TATCATGGGA

AAAATTGTGA

AALACTCGTAG

GCGAAATFrG

CGCGGCGACT

ATTGGTACGA

ACGTATACTC

GAACCTTACC

GCGACAAGGC

CTGCTGGTAA

GTGAAACCGG

GA.ACA=rTT

ATCAAGCCGT

GGAAAGATAA

AAAAATTAGC

CWATAAATC

ATAAAAACGG

ATCCAAACAC

TTGGG-'2ACTT

ATCGTTACGG

GAATGGGTAA

AAGGCACATA

GACTAAGATT

TGA'ITTAACG

TT=ACGAG

TGTGACAGGT

AGTGGATITA

ACTGGGGAAT

TTTTACAAAT

AGGGGATAAA

TAATGATGTG

AAGTAAATAt

CCAAGTGGTG

CGCGGCGCTA

AGATAATGCT

TCATCCAGCC

TTATCTGGCA

GCAGAAAkATG

TTITCGACA

GATGGCACAA

ACCGAAGTGA

GATGT7GAAC

GGACGTGAAG

ATTTTAGGAA

ACAGCGGCCA

CGGACTGGCA

CTAATIAT

AAAATACTGG

AACGA2ITT

GTAAAGACNA

TTTACGTGGA

CATTAACCAA

CAACCTTGTG

ATCAAAA=T

AGGAACCCGT

TGG'ITTAGCA

GGAAACCCGT

AATTTCGCGG

ATTTACTTAC

ACACATCCCT

TCAAACTGGC

GAATGGCACA

CATTAGTTTA

GAATTTTCA ATTACAAAC AGGTGAACC GACAATAACA AGATTCGGGC CCATGTATCT GAATTAACGG AACTAAACAA TCATCGATITT GGTAATGATA TTCAAGTGTT CGTGGAACAT

T

EF103-4 (SEQ ID NO:400)

I{AANPNSATA

TRGDKATKIL

DEGTFFVTGD

NLGKHQNNGQ

SGTDWQGTRV YDAAGNOLTA ENANFIGL~j( YDGETGFYEF FDKNTG;ETRG GTKRILI SRT QNYQAVVDLT EVSKDXFTYI', RLGKDKLGND VEVY-VEH IPY 200 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

HGKKLAFTNG REALTN'QTGK IVTNKSGDKI LGTTLWNGTK VVDKNGNDVT AANQNFISLA KFDPNTSKYE FFNLQTGIETR GDFGYFQVVD NNIRAHVSI GTNRYGAALE LTELNNDRFT YTRMGKDNAG NDIQVFVEHE PYQGTYHPA EF104-1 (SEQ 10 NC:401) TGAAAGGGGA TTAGTATGAA GCACAAAAT1' TCGGGTGC se 0.

GAGACCACG

TTAGCAACAA

ACAGACGCGA

GAGGACAAGG

GATGAACTAC

GAGGTTCACC

AACACAGCTr

TCAACTGTCA

AAAACATTAA

AAAAGTTATC

AAAAAAACCT

ACCAGAACGA

AATCAAACAT

A.ACTTGGAAC

ACGCAAACCT

ATTGCTGTAA

ACAATTTATT

ACAAAAATG

ACTCCTAAGA

TATATCAGTT

ACAAAAGTGG

AAATrTAccG

AAAACTGAAG

ATTTCTCAT

GTAATTGGTC

GTTGTTAATC

TCATCAAGAA

ATTAAAGTAA

TACAAGTTAG

ACTACGCCAA

TTGCCAAAAG

TI'AACTCCAG

CAAAGTAGTA

GTCCGTACAA

GATCAGGTAC

ATGACGCCAA

ACGTTTGATT

TGGATCGACG

AATGAAGGCT

CAAGCGGCGA

GATAATAAAA

AATGCTCAAA

TTAGAGAAGA

ATTACCATTG

ATCGACTCTG

ATCACATITGA

ATACAGCAALA

CGACTACTrC

GCACAACACC

ACACCTCACT

TTGACAGTAT

GCGAAFI'AAA

ATGCACCTGG

GCGGAGAAGT

GTATTACT

TAACAACAGA

ACTCGTTTGA

CGGGG ETAGA

TAGAATTATI'

CTCAAGTIT

TGTTAACACC

CTGTTCCAAA

TAGAGAGTGC

GGTCAATTTC

CGAGTCAAAC

'ITCAA.AGCAA

GTCAACAAAT

CGTATGGTCC

GTrCGCAACCT

TAACAATGGA

CTAATAAAGC

CTTTGAATGC

CAACTGTCTC

AGCATCCTAA

GAACGGATTA

TAACCAACGA

ATAAAAGTAT

TTGATACGAC

AAAATCAATT

AA.ATTCCAGC

ATTCAAITTA

ACAGCCCTGG

TGGAAAAAC

TCCCAACTCT

CTGCTTCGGT

ATCCAACAT l

CACATCGTGI

TCGATTTGAA

CTACAAACGC

AATACAATAC

AAACATTGAP

AATTCTCAGP

GAAAAAAACT

CCTAAATGCC

AAAAGAGGCA

AGAAATGAAT

TCCAGTGAT

TAATGA.AAAA

GAGTCTTGCG

TACAACACCG

ATCAGTTTAT

GACAGGCATT

TAATCAACGA

GGCGGAACCA

'TTTATATGAA

TGGCGAAT

AACAACAGAG

CAGTTACGAT

TGGCAAAGAT

CATGAATCAA

TTTTC?11'TG

TATCCTGTAA

GAGTTATCGA

CA.ACCAACTG

GCTGCTACAC

GCCfCTGCCAG

CCGATTGGTG

GTAACCGCTA

ACAGTTCAAT

GGCGCAACTT

TGATGFI'GAG

CAACGACAGA

ACTCAACACC

CAACAACTGA

CATCTGAACA

ATCTPCAAGC

GA.ACAGAATA

CGTTCCAATT

TACCAGAACA

GGGCAGTCGA

GTTTCAGATA C'FTCC'ITTAA AGTAGAACTA TTAATCAAAA TTGAAACTCC AGGAAAAAAT

CAAGTGGAAC

TTT-TATAATT

ACGCCACGC

GTCGACATTA

TATACATTAA

CAAAAAGCCT

TTCGGACTAT AACTACTTAT TTTGAAAAGT ACGACAGGAA

TATACTTCTC

AGCACAAACA

ATCTI'TACCT

ATCGCAAACC

ACAAACAACG

GCCAATTACA

CAGCCAAACA

TGCTGTTGGA

T11AGCI'AT

TGCGGCGACC

AAGTAAAGTA

AGGGAAATAT

CGTATTAGAG

TTTATATGAA

CACTTAACG

TTTTGACAAG

CATTCAATTA

TGAAACTGCT

AGTTATGGGA

TTATCTTTCA

TACAGTAACG

AATCCAAATT

CCCAGTCGAT

ACTAACTACT

CCTTGTCAAT

TGGCGCCGAT

TCCACAATAC

ATATCCAACG

CAACAAACGT

TTATATAACA

TTCTGTTCA-A

AAAAAATGTA

G.AAAAATCCA

TTCTATTACC

TGCGAAAGTC

GGTCTCTGCA

CCAGATGTCT

AGGTGATGCG

ATTGCTGAT'C

TATGTAACAA

AGTACAAACG

AATGTAAAAT

GCCACAAAAG

AAGGACATTA

GTATCCGATC

TGGGGTAAT1T

AGCAAAGAGA

TTACGAGCTA

CCAACGTCAG

CCAATTGGTT

ACGATACCGA

AATAGTAAGC

GCACGAAATG

GTTCTTTTTG

TGGGACC.GCG

ATTACTTTTG

TACATTATTG

GGGACAGCGA

~AMAGCC'I

ACAAAAACGA

ACGATTGAAM

GTGAALAGCCG

ATITI'AAAG

AACGCTGGCC

GCTAGCAACA

GAAGGTATM

CAATTCAATA TAACGAACGA TAGACCGGAC GTTAACTGGC CTGTCTTrGG AAAACAAGAT ATGGTCAAAT TTTACCAGAA GCGATAATTC ACTCGGCCGG ATTCCTTATC GATTAATCGG ATTCGCAGCA GTATCCAACA CTAAACAAAC AACCGATTTT GTGAAATGCG TAGTATGTCC TTTATGGCAC GTTAACAGAA GTCAAGAAAT TAAGAATCCT TGGAAGACTA TTTGATATT ATAGCTATT AAGAATAAAT ATCTATCATr AAGTACACCT AATATATTGA ACCAATTAGT ATGATCAAAA TGGTGCCTAT AACCGATTCA AAATTTAGAA CAAAAGAAAT TTATITTTAT ATGGITCAGT TATTAAGTTC TTAATTATrGT GCCAGATACT TAACAATCAG TGCTGAAGGT GTGGTTCTGA ACGAACACTI' AT==1TTGA CTCACTA.AC ACAT=TATGA TGTTTCAAAC GTCAATACTT TGATAAACCA ACGAAAATAC CAATAGTTAC AGTATAAAAA CGCCAATGGC AAGAACCACA ATCGAATAAT TAGACATI' GAGTGCAACA CAGTAACAAC AAAAAATATT TAACACCAAA AGGCACAACC TGUCCAGAAGA TGCTTATTCA ACTATACATI' GACAGAAAAC AAATCTATAC AGAA.ACAACA AAAAAGTCAC CACTGCGCCA TTI'ATTTAGC AACTGCCACA 201 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.fiaecalis Genes.

TICTACACGC

ATTGATAATG

=TGAT-GCCA

TCCGTGGATG

CTAAAAATTC

ATTTATGTCG

GGTCAACACG

GGCGTTTATC

AAACCCGACC

AAACCAGAAG

AAAATTGATG

AAAAATGGCA

GTTAAAGATA

GCAACAGACA

GATACTAGCA

GCTAATGTGA

GTGGGCGATA

GATGTCCCGT

TATGAAATI'G

GACAGTCAGT

GATAACTTCG

CTTCAGGGAA

ACAAAAGAAG

ACAACGATT

AAA.ACAGGTC

ATA.ACGTAGA

TCACGCATAC

TCATGACAGG

AAGAGTATTT

CATTAACGAA

GCGATTCATG

TTCCCTI'CGA

CAA ETA 1TA

AATCTAAGT

ATAATTTCGT

TTCAGGGAAC

NAAAAGAAGC

CAACGATTTA

AAACAGGCCA

AAGCAGGCGT

CTGTCAAACC

AATGGGAACC

TTGAAAAAAT

TCTATAAAAA

TAGAGGTCAA

TTTCCGCGAC

CAGTGAATC-T

CGA.AAGCAAT

ATGTCGGCGA

AAGATGTCCC

GGATGAAAAC

AGCAACCGAA

TGAITATACT

GACAGGAAAA

AACAATTGAT

GAAACCAGA.A

AAAAATCACT

CAGTGACGAA

AGAGGTCAAA

TTCAGCCACA

CAAGCAATTG

TTrTACAACAG

TI'GCGAGTAA

GCCATTAAC

CACAGTCGTT

GAGAACTTTG

GT~rCAGGTC

GGTAA.AGAAG

GATACAACGA

GACAAAACAG

AGTGAATG ET GATAAAATAG GAAAGCAATC GTCATrGTCC TGTTGGTGAT TCGTCGAAAC AGACGTTCCG TTTGAAAAkAA TTATCCAATC GITTACAGTT CGACCAATCT AAGTTAGAGG AGAAGATAAT TTCGTTTCAC TCACGTTCAG GGAACAGTGA TCGCACAAAA GAAGCGAAAC AGATACAACA A=rATGTG AGACAAAACA GGTCAAGACG TCATAAAATA GGCGATTATC CGTTCATGTC CGTGATGATA

CGAAGGTTTC

ATGAAAAAGG

CGAATGTAkCC T'3GTCAAAGG

TACAAGTCAA

=TCAGCAAC

AAGTTGATAA

AAACAGCCTA

'ITTATGTTGG

GTCAAGACT

GCGATTATGA

GTGATCACAG

CAGAAGATAA

TCACT-CTTIC

ACGAAGGTA.A

TTAAAGATAC

CAACAGACAA

ATG=GATAA

CAATCGTTCA

GTGATAAATG

TTCCGITI'A

AAATGTCTA

GTCGTI'TACA

TTTTGAACTA

TCAATACTCC

GCAGGAATAT

AGACAACCAA

AGATTCAACG

AGATAAAACA

CANCAAAGCA

TGTGACCGTC

TGATTCGTGG

NCCGTTTGAA

AATTGTCTAT

TCAGTTAGAG

TTCGTI'TCA

AGGTCAAGTT

AGAAGAAACA

AACGATTTAT

AACAGGTCAA

AATAGGCGAT

TGTCCGTGAT

GGAAGCAGAA

AAA.AATTGAT

TAAAAATGGC

AGTCAAGGAT

TTCNTGGANA

ATTCGAAAAA

CCAGALAGNGA

ATCACTGTT

ACTTTGTTTC AGCNACAGAT 9 9 @9 .9 9 9 99 9 9 .9 99 9. 9 9 99 9 9 9 EF104-2 (SEQ ID NO:402) M4KKKTF SFVMLSILLA QNPGFAVNAY AVTTTEAQTE TTOTAKEAE LSNSTPSLPL 99*9 9 9 *9*9 9. 9 *9 ATTTTSEMNQ PTATTESQTT ELLIJSMSLAP IGGTEYSQTE TVSCEVTG IG ATAVDAATK KTYSFDLYEQ VEPIQYNERT LEPQVFSYDV DINGQILPET IYLESASDYN YLYSQQYPTT ISFQSKGKYY VTIYGTLTET TEGGKLTLTA TKDSYLRINI VNPLNAETAW GNYDQNGAYS KLGTDYTVTP TSDGSVIKFT TPVDTTVTTN SKRGSERTLQ QVDS IYPQYW DRGQYFDKPM IDVPTLYITG TAREPQSNN NKTHRVKNPT IELTPKGTTN TIEYNTVSAN AGQ IYTETTI YTHNVEDENQ AIAKVSFELI VDEEYLTGKA IKLVKGDNQL QDVPFEKITV SGQVDNXKAG PEDNFVSATD KTGQDVPFEK KDTTIYVGDS WKPEDNFVSA NVTVKPDQSK LEVKDTTIYV E IVYKNGTKE AKAIVHVRDD

EASTTASSDA

VHRELNTrPV

TLSITFNQRV

RTTGLOGEIF

QTLLTPGKDY

KIGSISLKST

KVGQQIVLES

SDLTMDFDKK

SRTTVSVMGS

TPITNE IQI P

SSKNQFLVNA

TPNSPGYPTI

EGSASVSVQN

AQIDLNS ITV

DSETLNQMSA

DNVTHTATEF

KI PLTKTIDH VYPI IYSDEG

IDVQGTVNVD

TDKTGQDVPF

GDKWEPEDNF

SQLEVKDTTI

ATPSEQQTI'E

TATFQFAVGN

SDTSFKVE:LK

YNLDRTLMN

TLSDNSLGRI

TGTKQTTDFT

TNGQE IKNPK

DINLSLSTPV

REKP IQNLEI

IGFNYVPDSL

RNDSFDSLSV

TFDENTNSYT

EALDILSATQ

KGVPEIJAYSL

SKXKVTTAP I TrDEKGQYSF

SRLQVKDSTI

KEETA'xVIVK

KIGDYEIVYX

EKITVSGQVD

DKDTSLNEKA

TGYARGSVYT

SYLTTEAEPL

QTLELLTTET

AVTVPNMNQQ

AKTSQTSKVI

FTAYGPLYEN

IGPiWKAIQLV

KVKHPNYLSL

PKDKSIPVDT

RTXI PAGADV

FDFGKTNKRY

AANPTLKNVT

EKT'INGAKVI

TLKFSEGDAE

DAIMTGOYTL

YVGDSWKPEE

PDQSKLEVKD

NGXKEAKAIV

TSICAGVYP IV

LPDVQAPITD

VQLPEHLGYS

IKIETPGKNK

PGAVFGKQDN

KAYSLS INRT

ADREMRSMSY

VX<LEDYFDIK

SDQYIEPISV

RATKEIYFYY

IPITMSAEGL

LFDIYDVSND

I IEYKNANGW KTTVTTKNI D

FKDYTLTENI

GIVYLATATF

RVTNVPQEYS

NFVSATDKTG-

TTIYVGDSWI<

HVRDDSQLEV

YSYEGKEETA

TVNVDKIGDY

QDVPFEKIDV

EXNFVSATDK

VSATDKTGQD VPFEKIDVQG YVGDKWEAED NFVSATDKTG DDSRLQVKDT TIYVGDSWXP QGIVNVDKIG DYE IVYXKNGT KEAKAIVHVR TGQDVPFEKI TV 202 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faccalis Genes.

EF104-3 (SEQ ID NO:403) TGTAA CAACGACAGA AGCACAAACA 0 0 0s 0* 0 0 *0 00 0 0 0S 00 0 0 000 0 00 0 0 000 0 *04000 0 0000 9000 0000 0

GAGACCACTG

TTAGCAACA-A

ACAGAGGCGA

GAGGACAAGG

GATGAACTAC

GAGGTTCACC

AACACAGGTT

TCAACTGTCA

AAAACATTAA

AAAAGTTATC

AAAAAAACCT

ACCAGAACGA

AATCAAACAT

AACTTCGAAC

ACGCAAACCT

ATTGCTGTAA

ACAA'ITTATT

ACAAAAATTG

ACTGCTAkAGA

TATATCAGTT

ACAAAAGTGG

AAATTTACGG

AAAACTGAAG

ATTCTGATT

GTAATTGGTC

GT'rGTTAATC

TCATCAAGAA

A'FIAAAGTAA

TACAAGTTAG

ACTACGCCAA

TTGCCAAAAG

TTAACTCCAG

CAAAGTAGTA

GTCCGTACAA

GATCAGGTAG

ATGACGCCAA

ACGTTTGATT

TGGATCGACG

AATGAAGGCT

CA.AGCGGCGA

GATAATAAAA

AATCCTCAAA

TTAGAGA.AGA

ATTACGATTG

ATCGACTCTG

ATCACATTGA

TTCTACACGC

ATTGATAATC

TTrGCATGCC~b ATACAGCAAA

CGACTACI'C

GCACAACAGC TI ACACCTCACT

TTGACAGTATC

GCGAATTAAA

ATGCACCTGG IZ

GCGGAGAAGTC

GTATTACGTT

TA.ACAACAGA

ACTCGTTTGA

CGGGGTTAGA

TAGAATTATT

CTCAAGTTTT

TGTTAACACC

CqTGTTCCAAA

TAGACTC

GGTCAATIIC

CGAGTCAAAC

TTCAAAGCAA

GTCAACAAAT

CGTATGGTCC

GTGGCAAGCT

TAACAATGGA

CTAATAA-AGC

CTTTGAATGC

CAACTGTCTC

AGCATCCTAA

GAACGGATTA

TAACCAACGA

ATAAAAGTAT

TTGATACGAC

AAAATCAATT

AAATTCCAC

ATTCAATTTA

ACAGCCCTGG

TTrGGAAAAAC

TGCCAACTCT

CTGCTTCGGT

ATCCAACATT

CACATCGTGT

TCGATTTGAA

CTACAAACGG

AATACAATAC

AAACAI'GAA

AA ETCTCAGA

ATAACGTAGA

TCACGCATAC

TCATGACAGG

AAAGAGGCA G iGAAATGAAT C ~TCCAGTGAT G 'AATGAAAAA G ;AGTCTTGCG C ACAACACCG G .TCAG'ITTAT A

ACAGGCATT

['AATCAACGAC

.GCGGAACCA1 IrTATATGAA C rGGCGAAATT I kACAACAGAG CAGTTACGAT C rCGCAAAGAT

:ATGAATCAA

TTCGGACTAT

TTTGAAAAGTI

AAGTAAAGTA

AGGGAAATAT

CGTATTAGAG

T'IATATGAA

CACTT.TAACG

TTTTGACAAC

CATTCAATI'A

TGAAACTGCT

AGTTATGGGA

TTATCTI=CA

TACAGTAACG

AATCCAALATT

CCCAGTCQAT

AGTAACTACT

CCTTGTCAAT

TGGCGCCGAT

TCCACAATAC

ATATCCAACG

CAACAAACGT

TTATATAACA

7rCTGTTCAA

AAAAAATGTA

GAAPLAATCCA

TTCTATTACC

TGCGAAAGTC

GGTCTCTGCA

CCAGATGTCT

AGGTCATGCG

GGATGAAAAC

AGCAACCGAA

TGATTATACT

AGTTATCGA ACTCAACACC ATCTI'ACCT AACCAACTC CAACAACTGA ATCGCAAACC CTMCTACAC CATCTGAACA ACAAACAACG CCCTGCCAG ATGTTCAAGC GCCAATTACA :CGA'ITGGTC. GAACAGAATA CAGCCAAACA ;TAACCGCTA CGTTCCAATT TGCTGTTGGA CAGT-CAAT TACCAGAACA TrAGGTTAT GCGCAACTT GGCCTCGA TCCGGCGACC ;TTCAGATA CTTCCTTTAA AGTAGA.ACTA r'AATCAAAA TTGAAACTCC AGGAAAAAAT .AACTGGAAC CAA=,tAATA TAACGAACG-A ~TTTATAATT TAGACCGGAC GTTAACTG'GC ~CGCCAGGCG CTGTC-TTTGG AAAACAAGAT ;TCGACATTA ATGGTCAAAT TTTACCAGAA CATACATTAA GCGATAATTC ACTCGGGCGG :AAAAAGCCT ATTCCTTATC GATTAATCGG kACTACTTAT ATICG-CAGCA GTATCCAACA kCGACAGGAA CTAAACAAAC AACCGXTIT %TTGCTGATC GTGAAATGCG TAGTATGTCC L'ATGTAACAA T=ATGGCAC GTTAACAGAA AGTACAAACGCGTCALAGAAAT TAAGAATCCT A4ATGTAAAAT TGGAAGACTA TTTTGATATT GCCACAAAAG ATAGCTATTT AAGAATAAAT A.AGGACATTA ATCTATCA'IT AAGTACACCT GTATCCGAtC A.ATATAITTA ACCAATTACT TGGGGTAATT ATGATCAAAA TGGTGCCTAT AGCAAAGAGA AACCG-ATCA AAATTrAGAA TTACGAGCTA CAAAAGAAAT TTAT'TMAT CCAACGTCAG ATGGTTCAGT TATTAAGTTC CCAATTGGTT TTAATTATGT GCCAGATAGT ACGATACCGA TAACA.ATGAG TGCTC-AAGGT AATAGTAAGC GTGGTTCTGA ACGAACACTT GCACGAAATG A IrCTTI'IGA CTCACTAAGC GTTCTT7=T ACATTTATGA TGTTTCAAAC TGGGACCGCG GTCAATACT TGATAAACCA ATTACTTTTG ACGAAAATAC CAATAGTTAC TACATTATTG AGTATAAAAA CGCCAATGGC GGGACAGCGA AAGAACCACA ATCGAATAAT AATGAAGCGT TAGACATII GAGTGCAACA ACAAAAACGA CAGTAACAAC AAAAAATATT ACGATTGAAT TAACACCAAA AGG-CACAACC GTGAAAGGCG TGCCAGAAGA TGCTTATI'CA ATTrTTTAAAG ACTATACATT GACAGA4AAC AACGCTGGCC AAATCTATAC AGAAACAACA GCTAGC:AAGA AAA.AAGTCAC CACTGCGCCA GAAGCTATTG TrATFTAGC AACTGCCACA CAAGCAATTG CGAAGGTTrC I=ITGAkACTA TTTACAACAG ATGAAAAAGG TCAATACTCC TTGCGALGTAA CGAATGTACC GCAGGAATAT GCCATTAACC TGGTCAAAGG AGACAACCAA CACAGTCGTT TACALAGTCAA AGATTCAACG TCCGT'GGATG AAGAGTATTT GACAGGAAAA CTAAAAATTC CATTAACGAA AACAATTGAT TABLE 1. Nucleotide and Amino Acid Sequences of E.jftecalis Genes.

ATTTATGTCG

GGTCAAGACG

GCCGTIATC

AAACCCCACC

AAACCAGAAG

AAAATTGATG

AAAAATCCCA

GTTAAAGATA

GCAACAGACA

CATACTACCA

GCTAATGTGA

GTGGGCGATA

GATGTCCCGT

TATGAAAI-rG

GACAGTCAGT

GATAAC ETCG

GTTCAGCAA

ACA.AAAGAAG

ACA.ACGAT

AA.AACAGGTC

GCGATTCATG

TTCCCTTCGA

CAATTAMTTA

AATCTAAGTT

ATAATTTCGT

TTCAGGGAAC

NAAAAGAAC

CAACGATTA

AAACAGGCCA

AAGCAGGCGT

CTGTCAAACC

AATGGGAACC

TTGAAAAA.AT

TCTATAAAA.A

TAGAGGTCAA

TTTCCGCGAC

CAGTGAATGT

CGA-AAGCAAT

ATGTCGGCGA

AAGATGTCCC

GAAACCAGAA

AAAAATCACT

CAGTGACGAA

AGAGGTCAAA

TTCAGCGACA

AGTGAATGTT

GAAAGCAATC

TGTT-GGTGAT

AGACGTTCCG

TrTATCCAATC

CGACCAATCT

AGAAGATAAT

TGACGI'CAG

TGGCACAAAA

AGATACAACA

AGACAAAACA

TGATAAAATA

CGTTCATGTC

TTCNTGGANA

ATTC

GAGAACTTT

GTCAGGTC

GGTAAAGAAG

GATACAACGA

GACAAAACAG

GATAAAATAG

GTTCAMTCC

TCGTGGAAAC

TTTGAAAAAA

GTT*ACAGTT

AAGTTAGAGG

7T"CGTTTCAG

GGAACAGTGA

GAAGCGAAAG

AT=ATTGG

GGTCAAGACG

GGCGATIATG

CGTGAT--ATA

CCAGAAGNGA

TTTCAGCAAC

AAGTTGATAA

AA.ACAGCCTA

TTTATGTTGG

GTCAAGACGT

GCGATTATGA

GTGATGACAG

CAGAAGATAA

TCACTGTTTC

ACGAAGGTAA

TTAAAGATAC

CAACAGACAA

ATGTTGATAA

CA-ATCdTTCA

GTGATW~TG

TTCCGTTTGA

AAATTGTCTA

GTCGTTTACA

ACT=TTTC

AGATAAAACA

CANCAAAGCA

TGTGACCGTC

TGATTCGTGG

NCCGIT=AA

AATTGTCTAT

TCAGTTAGAG

TTTCGTTTCA

AGGTCAAGTT

AGAAGAAACA

AACGATTI'AT

AACAGGTCAA

AATAGGCGAT

TGTCCGTCAT

GGA.AGCAGAA

AAAAATTGAT

TAAAAATGGC

AGTCAAGGAT

AGCNACAGAT

EF104-4 (SEQ ID NO:404) VTTTE-AQTE TrDTAKKEAE LSNSTPSLPL 0@ 0S S 0S

S.

S. S 0O @0

S

@0 @5 S 0

S

S.

0@ S 0 0

S

OS@9 0

OS..

S.

SSSS

0 *0e0 4 000@

OOSS

0 0S@S 0e 0 00

S.

ATTTTSEMNQ

ELLDSMSLAP

TVSGEVTG IG

KTYSFDLDYEQ

LEPQVFSYDV

IYLESASDYN

ISFQSKGKYY

TEGGKLTLTA

VNPELNAETAW

KLGTDY'IVTP

TPVDTTVTTN

QVDS IYPQYW

IDVPTLYITG

NKTHRVKNPT

TIEYNTVSAN

YTHNVEDENQ

VDEEYLTGKA

QDVPFEKITV

PEDNFVSATD

KDTTIYVGDS

NVTVKPDQSK

EIVYKNGTKE

QGTVNVDK IG

TGQDVPF

PTATTESQTT

IGGTEYSQTE-

ATWAVDAATK

VEPIQYNERT

DINGQILPET

YLYSQQYPTT

VTIYGTLTET

TKDSYLRINI

GNYDQNGAYS

TSDGSVIKFT

SKRGSERTLQ

DRGQYFDKPM

TAKEPQSNNN

IELTPKGTTN

AGQIYTETTI

AIAKVSFELI

IKLVKGDNQL

SGQVDNXKAG

KTGQDVPFEK

WKPEDNSFVSA

LEVKDTTIYV

AYXAIVHVRDD

DYE IVYKNGT

EASTTASSDA

VH-RELNTTPV

TLS ITFNQRV

RTTGLDGEIF

QTLLTPGKDY

KIGSISLKST

KVGQQ IVLES

SOLTMDFDKK

SRTTVSVMGS

TPITNEIQIP

SSKNQFLVNA

TPNSPCYPTI

EGSASVSVQN

AQIDLNSITV

DSETLNQMSA

DNVTHTATEF

KIPLTKTIDH

VYPIIYSDEG

IDVQGTVNVD

TDKTGQDVPF

GDKWEPEDNF

SQLEVKDTTI

KEAKAIVHVR

ATPSEQQTTE

TATFQFAVGN

SDTSFKVELK

YNLDRTLTGN

TLSDNSLGRI

TGTKQ~rDFT

TNGQEIKNPK

DINLSLSTPV

KEKPIQNLEI

IGFNYVPDSL

RNDSFD)SLSV

TFDENTNSYT

EALDILSATQ

KGVPEDAYSL

SKKKVI'TAPI

TTDELKGQYSF

SRLQVKDSTI

KEETAYV'rVK

KIGDYEIVYK

EKITVSGQVD

VSATDKTGQD

YVGDKWEAED

DDSRLQVKDT

DKJ)TSL.NEKA

TGYAPGSVYT

SYLTTE-AEPL

QTLELLTTET

AVTVPNb'NQQ

AI(TSQTSIZVI

FTAYGPLYEN

IGPNKAIQLV

KVKHPNYLSL

PKDJKS IPVDT RTKI PAGADV

FDFGKTNKRY

AANPTLKNVT

EKTTNGAKVI

TLKFSEGDAE

DAIMTGDYTL

YVGDSWKPEE

PDQSKLEVKD

NGXKEAKAIV

TSKAGVYPIV

VPFEKIDVQG

NEVSATDKTG

TIYVGIDSWXP

LPDVQAP lTD VQLiPEHLGYS

IKIETPCKNK

PGAVFGKQDN

KAYSLS INRT

ADREMRSMSY

VKLEDYFDIK

SDQYIEPISV

RATKEIYFYY

IP ITMSAEGL

LFDIYDVSND

I IEYKNANGW KT'IVTTKNI U

FI(DYTLTENI

GIVYLATATF

RVTNVPQEYS

NFVSATD)KTG

TrIYVGDSWK

HVRDDSQLEV

YSYEGKEETA

IVNVDKIGDY

QDVPFE I DV

E-XNFVSATDK

EF105-1 (SEQ ID NO:405)

TAAATGAAAA

G TCCrCTG

AGCACAGATO

AAACAGTCGT

AAGCGGCGAC

GGACGGATCC

CTACTCCTTG TTATTCGGAA CAATGTTGCT TGGCGCCACT GGTCGTTI'T GATAGCGAAC AGTCGATTGT TTTTACCCCA AGTAAATCCA GAAAATCCCG ATCCAGAAAA ACCAGTTCGA 204 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faccalis Genes.

CCAGTCGATC

GATTATGCCT

TTTGCCAGAG

AATTATGTAC

CAAAATGGTC

GCCITACTG

GCAAACATTC

ACCGGCGCCG

CAACAAGGAC

AAAACCCCTA

CCAGTAAATA

CAACGAATCC

CAAGTTTCA

CGCAAACCTA

AkAGTAAGTGA

AATTTCGTAA

AGCCCAGTGT

AATTAGATGC

GAACGTGGAT

AGCAAGTAAA

AGGATGCAGT

ATGGAGGGAA

TGATGGACCT

TTTTGGGAGT

TAGAAATCCA

=TIACGGGGA

TGCAGAAACA

TCGCTCAAAT

TGCGGGCGCA

CACGCTG'GC

TGCCACAATC

ACAATATAAA

ATAA

AATCCAGGTA

AATGAGATAT

GATGGTTCAG

ACCAATGCTG

TTACACA-AAG

GCGACGGACG

GAAACTGTI'

GGGCAAGCAG

ACACGGGCAA

ACAACATTGA

CCCCTGGTCC ACICCATC

CGAATAAGGA

C:AAGTGAATT

GCTGGGTTTT

AATTAACAGG

TA'ITGCCGCC

TCATGCAAGC

AAAAACTGAC

TCTCACTAAC

CT=GCTACT

TCAAACGTAT

GGCAACTGCT

AAAAGTGAAA

CGCCACCOTC

AACTGCTACC

CCCAGAAAAG

CGAAAAAAAT

TGTTCCTGGG

TCAGATGTA

EF1OS-2 (SEQ ID NO:406)

MKKTVVYSLL

VDPTNPDGPN

YVQVSDLRGT

NIQLDAAGAE

TPKDAVQYKT

FGTMLLGATV

PGTPGPLS ID

NAGWVLKVKQ

TVVMQAPEKT

TLTWLLSDVP

PAEAATVVFD

YASSLDFGSN

NGQFRNAETL

GACTWITLWG

VNNGGK

SEQSIVFTPS

El SNXDQTYF

HKELTGAEVA

QAEKVTEKNQ

TOGTDPVNPE

ARAQTYRNPD

FTEPSVRSNA

QGQQVNATIT

NPDPEKPVRP

GSASELATAN

TDVLPPTATA

RAISLTVPGK

EF105-3 (SEQ ID NO:407) GGCGAC GGTCGTITTT GATAGCGAAC AGTCGAT1TGT TTTTACCCCA

ACCACAGATG

CCAGTCGATC

GATTATGCCT

TTGCCAGAG

AATTATGTAC

CAAAATGGTC

GCCTTTACTG

GCAAACAITC

ACCGGCGCCG

CAACAAGGAC

AAA.ACCCCTA

GGACGGATCC

CAACGAATCC

CAAGTTTGGA

CG-CAAACCTA

AAGTAAGTGA

AA=CGTAA

AGCCCAGTGT

AATTAGATC

GAACGTGGAT

AGCAAGTAAA

AGGATGCAGT

AGTAAATCCA

TGATGGACCT

TTTTGGGAGT

TAGAAATCCA

TTTACGGGGA

TGCAGAAACA

TCGCTCAAAT

TGCGGGCGCA

CACGCTGTGG

TGCCACAATC

AC

GAAAATCCCG

AATCCAGGTA

AATCAGATATI

GATGGTTCAG

ACCAATGCTG

TTACACAAAG

GCGACGGACG

GA-AACTG LTG

GGGCAAGCAG

ACACGGGCAA

ATCCAGAAAA

CCCCTGGTCC

CGAATAAGGA

CAACTGAATT

GCTGGGTTTT

AATTAACAGG

TATTGCCGCCC

TCATGCAAGC

AAAAAGTGAC

TCTCACTAAC

AC CAGTTC GA

ACTITTCCATC

TCAAACGTAT

CCCAACTGCT

AAAAGTGAAA

CGCCACCGTC

AACTGCTACC

CCCAGAAAAG

CGAAAAAAAT

TGTTCCTGGG

S EP105-4 (SEQ ID NO:4D8) ATVVFD SEQSIVFTPS TDGTDPVNPE NPDPEKPVRP VDPTNPDGPN PGTPGPLSID YASSLDFGSN EISNXDQTYF ARAQTYRNPD GSASELATAN YVQVSDLRGT NAGWVLKVKQ NGQFRNAETL HXELTGATVA FTEPSVRSNA TDVLPPTATA NIQLDAAGAE TVVM~QAPEKT GAGTWITLWG QAEKVTEKNQ QGQQVNATIT RAISL'IVPGK

TPKDAV

EF106-1 (SEQ ID NO:409)

TAGTCGTTTA

TTAGT'I-GTrG

AATCCCAATA

GAAGGATTAT

ITAACAGATG

TATGAATTI

ACCGCTGGTA

GGTGTATACC

GATAAAAACG

TGAAGAAAAA

GAGCAGGAGG

A=~AGGGGA

CAGAAAAAGA

AAAATCAAAA

TTGATAAAGA

TTACAGATGT

CACI'AGAAC

GAAATGATAT

AATCGTTGGT

G.GCTrTGACG

ITTACCTGAG

'TGGCCTGCA

CCTATTACAT

GACTGGAGAA

TTCCCGTCTT

TTTATACCAA

TGAAGTTTTC

ACAATTACGT

GCAGAAGCAT

TATTTACGTT

ACACGCGTT

GCAATCAAAT

TCAACAGGAG

GTAA'TATT

GATACTTTITA

GTAGAAAACA

TGTGCCTTT

ACGTTCCTCA

CAGTI'GGTAT

ATGATCGAAA

TTGATGCAAC

ATGAAGGAAC

CTGAAACCAA

CGTATAGACA

AAGCAACCTC

AAGTGCGTTA

AAGCGTAGAC

TAGACAAGAT

TGGGAATGAC

CACTAGTTTC

CTTCT=ATG

AAATTATCA.A

GATrOGGGAMA

AGGACCAGTT

205 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

TATGGTCGTC

CCCCGTGCCA

ATTGGTAAAA

CATAAAGATG

GACCA.AGATA

TATGGCTACT

AATCGCTATG

CGAATGGGTA

CAAGGAACTT

CGCAGCCATA

TGACAGAACA

CTTCCTTTGA

GCAATGACGT

CCCAAATA

'rrAAAGTAGG

GCGCTGTCTT

AAGATAACGA

TTAATCCTGA

CCCCAATAAT CGTCCCAGAA AACAGGCCAG ATTGATGTAA TGGGACACCG CAACTTCTTT AACTTCGGCC AACCAAAACT TGAATTTTTC AATTTACAAA AAATCAAAT AAATrCCGTO AGAGTTAACA GAArrGAATG AGGAAACGAT ATCCAAGTCT ATTTACCI'T TAA

CACTAGAAT

ATCGACAAGG

GGAATGCCAC

TATCAGCTT

CTGGTGAAAC

CCCATGTTTC

ATAATCGTTT

AtGTGGAACA

CACGAATGGA

GGATGAAATT

AAAAGTAGTC

AGCGAAATTT

TCGTIGGCGAC

CATTCCAACC

TACGTACACA

TGAACCATAC

EF106-2 (SEQ ID NO:410) MKKKIVGT ITLLALSALL VGGAGGALTA E-AYVPQSVDN PNNLGDLPEY LRSVGIRQDE GLSEKDWAGT RVYDRNGNDL TDENQNLLH-A IKFDATTSFY EFFDK8TGES TGDEGTFFMT AGITDVSRLV IISETKNYQG VYPLRTLYQD TFTYRQMGKD KNG-NDIEVFV ENKATSGPVY GRPQPYPNNR PRTLEFTNGR RANTEQTGQI DVNRQGDEII GKTSFOGTPQ LLWNGTKVVD KDGNDVTSAN QNFISLAXFD QDSSKYEFFN LQTGETRGDY GYFXVGNQNI( FRAHVSIGTN RYCAVLELTE LNDNRFTYTR MGKDNEGNDI QVYVEHEPYQ GTFNPEFTF EF10O6-3 (SEQ ID NO:412.)

S.

S S

S

S.

S

S

555555

S

S

55S5 5555 5555

S

5*55

S

AT ACGTTCCTCA AAGCGTAGAC

AATCCCAATA

GAAGGATTAT

TTAACAGATG

TATGAATTTT

ACCGCTGCTA

GGTGTATACC

GATAAAAACG

TATGGTCGTC

CGCCGTGCCA

ATTGGTAAAA

GATAAAGATG

GACCAAGATA

TATGGCTACT

AATCGCTATG

CGAATCGTA

CAAGGAACTT

ATTTAGGGGA

CAGAAAAAGA

AAAATCAAAA

TTGATAAAGA

TTACAGATGT

CACTTAGAAC

GAAATGATAT

CGCAGCCATA

TGACAGAACA

CTTCCTTTGA

GCAATGACGT

GCAGCAAATA

TTAAAGTAGG

GCGCTGTCTT

AAGATAACGA

TTTACCTGAG

TTGGGCTGGA

CCTATTACAT

GACTGGAGAA

TTCCCGTCTT

TTTATACCAA

TGAAGTTTTC

CCCCAATA.AT

AACAGGCCAG

TGGGACACCG

AACTTCGGCC

TGAATTTTTC

AAATCAAA.AT

AGAG'ITAACA

AGGAAACGAT

TATTTACGTT

ACACGCGTTT

GCAATCAAAT

TCAACAGGAG

GTAATTATTT

GATACTTTTA

GTAGAAA.ACA

CGTCCCAGA.A

ATTGATGTAA

CAACTTCT

AACCAAAACT

AAT ITACAAA

AAATTCCCTG

GAATTGAATG

ATCCAAGTCT

CAGTTGGTAT

ATGATCGAAA

TTC-ATGCAAC

AT--AAGGAAC

CTGAAACCAA

CGTATAGACA

AAGCA.ACCTC

CACTAGAAT

ATCGACAAGG

GGAATGGCAC

TTATCAGCIT

CTGGTGAAAC

CCCATG'TTC

ATAATCGTrT

ATGTGGAACA

TAGACAAGAT

TGGGAATGAC

CACTAGTTTC

CTTCTTTATG

AAATTATCAA

GATGGGGAAA

AGGACCAGTT

CACGAATGGA

GGATGAAATT

AAAAGTAGTG

AGCGAAATTT

TCGTGGCGAC

CATTGGAACC

TACGTACACA

TGAACCATAC

EF106-4 (SEQ ID NO:412) YVPQSVDN PNNLGDLPEY LRSVGIRQDE GLSEKDWACT RVYDRNGNDL TDENQNLLHA AGITDVSRLV IISETKNYQG IJYPLRTLYQD GRPQPYPNNR PRTLEFTNGR RAMTEQTGQI KDGNDVTSAN QUFISLAKFD QDSSKYEFFN RYGAVLELTE LNDNRFTYTR MGKDNEGNDI

IKFDATTSFY

TFTYRQMCKD

DVNRQGDEI I

LQTGETRGDY

QVYVEHEPYQ

EFFDKETGES

KNGNDIEVFV

GKTSFDGTPQ

GYFKVGNQNK

GT

TGDEGTFFMT

ENKATSGPVY

LLWNCGTKVVD

FRAHVS IGTN EF107-1 (SEQ ID NO:413)

TAAAAAACGG

ATANATANAA

CTTTATAGAG

AATTGGAAAA

CACTCAATAT

AAATGCTAGT

ACTATAGATT

GATGGCTACT

GTCAAAA TT

TATCAGTATC

GAATTF1TTAC

TGTTGGGTTA

GAAATrTCAA

GATAATAACA

ATAGAAAGAA

AGTTGTTCTT

GCTGTCGT C?1'TCCTAAA GGATACTGAT TAAGAAAGGA GGAGCAAGAT GAAGCGAGTA TGTTCATGGA TTCAGTrGCTT 206 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

a a a a a a a.

GGTGTGACTG

CAGTCGAOTG

GCTAGTGAAG

GG TATTCT

TTGTTTGATC

GAGAAATTAC

ATTCCAACGT

GGCGTTTCTG

AGTACGTTTT

GGGGGCAAAG

GAAAAAATGC

ACGGCACCAG

ACTAGTTCGC

CGTGTCACGG

ACGCAGGATA

TTGCCTGAAC

GGGAATGCGA

GATCACAATG

CCAGCGAGAG

AAGTTTAAGC

TTTCCCACAA

CAAAAAGTCT

AGTGTTGAAA

GGGGATAAAA

AACA=Tr TA

CGCTATACTG

AGTATCTATT

TATTATTACT

ATCACGCCAC

GTCTACACTG

TTCCA.AGGCI

AGTTTTACAC

CCCACCGCGC

GATTACTOOC

AAGCCAACGC

ACGGGTCAA.P

GTGTCCATC;

GGAACCGCCC

AAC'TrGGC;

ACGAGTCCTC

ATT1CAGGAJ

ACTCGCAAC(

CTATCGCAA(

ACGGCCGCTC

GGCAAGAMC

CAACAGTrC

GAAGTGCCA(

TTAGTGACT(

TGTTAGCGGA A ACGAAGCGAG C

CGAAAACAGT

CTGTGGAAGG G CCGACCCAAT AACGAACCCC C CACCAGC TT' AAGTGTCG1' ATACAGGAGA C TGACGAACTA CCAATAAGCT C GAGGCGTTAC C ACCAAAGACG I

AAAAAITGA

AGAAAACCAT

GTTACACAGG

AACCTAGCAC

ACGATTGCA

AAGCTTTGTT

TAAGTGCGAC

TTGATGGAAT

ACACACGCCC

TTCCTAAGTA

CCAAGTATCC

CTAr-TTTAGT

CCCCCCGTTC

CAGGAACTGC

TAGAAAATCG

CAACTGGCTT

TCGCAAAAGC

GGTTTAAAGG

CAACTTTIAA

1AACTAACGTT

AGGCGCTACT

CAACTTCGC

ACACCAAAGC

CGITGGATCA

TTACAGGAAA

SGTTTAACTGC

ACGGCGACGG

k GTAAGCAACA

:CTTATTI'ACG

CAAAATCAGC

CTGCCAGCTT

k. GCACCGTGAC k CAGGCAGTGA 3CCAACCAAGG 3 GCCCCTAA

CAGACGACG

.CCGCCACAG

CGCGAGATT

'ACGATGGCG

CTI'GAGCCC

,'AAATATGAT

'ACTATTCCT

:ACTACGCAA

MTGGGCGCC

;ACGTTTGGT

TATGCGCTrG

~GGAACAGCG

;AATCCCGCA

L'CTAACAGGG

:AGTGATGGG

3TTGGA.AACC rGTGGTCTAT

CGGCTATGTT

CATGGGTOGAA

:GATATGCCA

AGACGATGGC

F1ACCAGACC

AGTACCAAAM

GGGAANCNCI

TI'ACTGGANC

TGGGGGCAAC

GCGGGTCACIJ

TACACAAGG;

TTrTGCCGAAC

CAAAACCAAC

TG-ATGAGGA(

AACAGGTGCC

GAAGAACAC

GGCTGGCAT(

TTTTCCTGTI

GCCTTTACC,

TCCTGGTCA

CAAAGATAC

C ITTATTAG ATATGrGTTT

CCTGAATAC

CACAGACAG

TACCGATTA

'rrrAACCGC

CGTCTATCA

TATGGCTGC

CAAACAACCG

GAAACGGCAA

CCCTTTTTCT

GTCAATCTTI'

ACCACTG-TGA

ACAAATAACC

AAGGAGTATC

TATCCAGTGC

AATGCGGAGA

CICTA.AAAAGA

ACCTATTT 1T

GGTCCTGTGT

GGCGGGGCGA

GAGGATTTTA

A.AGACGTATI'

ACCAAAACGC

GAAGAAGCAG

GATCAGCAAG

AGTGACGGAG

GCAAGTCAAM

ACAATCGTAP

ATTTCGATT

GA.AGTGCGCC

GCGTATAACI

TGGGGCCCC;

TATAATTTAI

7GAACATTTTC kAATCAGCTA( 3ATCTACCAAC 3 CCAGCAACAr.

-GACATGACC(

:GTTGACATAj P, GGCGAAGCGI

ZGGCGCACCC,

:ACCAAAGAA,

A GCTGGCGGT A GTTTTAACC G GTCCGTATT T ACCCCGACA C AAGAAGGCC T AGCCAAGCC C TTGCCAACA .C AACCAACCA C GACAATACC ,G TTGGACTTC C CAACAATAC ACGATTACT GGGGCGACGG

AGCAALGGAGT

AAGAGTCACA

GAATTGCTTC

TTCTGGAGGA

CCACGTTIC

ATGGCGGAAA

AGAATCCAAG

AAAGCCTGGA

CAACGGT

CGGAATTAGA

CG =TAAATA

ATCGAGATGT

ATTATTATTP

TTCCTGCGCC

CTTTACCCA

TATTTAA-AGG

CTAGTTATGC

TGATGAAAAC

GAAACTTGAT

CCACAGGGGA

TAAAGAAATT

*CITrATGGCCCC

CACCAACTAC

*GTGGCATCGA

7TGACCCAAAA

CGA.AGACACT

CGACCCCTGA

TAGACGAAAG

TGGTGGACAC

CTGGTGAAAA

r' TAAAGACGAC

;CTCTCTACC)

k TCGAAAATGC

:CGTTA-ACCAC

4 ACACCATA' C AATGGACGA( C AATTAAAAAM 0 CTGATGTTGJ A AAGACTTAGj T T1'GA'ITTG( G CAGATTACMi A ATTGGAGTT A CGACCCGCT A CAGAAACCAI ZG CAACAGCCGG .A CGTTTGCTA 'C AAGCCGCCG

AGCAACATCT

GGCAACGGTC

TCGAGCGATT

AGACGGGACG

GITTTATGAA

GTTACTGTCT

TAATA=TAT

CATTCGACCA

TGCGAACGTT

GTTAACCAAT

TACGGTAGCT

CGGCGGTCCA

AACAAAGCGG

AGAAGGTTAT

AGAAGGCACC

TTGGTACAAA

GGTGACCtAT

CTATACGTTG

TAATCCCGCC

AATGACGACT

AGCCATCCCG

GCAAGAAGTG

TGCGTATACA

AAACCCCGAC

AAGTGCCACA

TTACTCAATG

TGGCACCATT

TGGCGCAAAA

TGAAAACTAT

AACCTATATC

AACGACCCCA

AGAAGCGATT

CGCCACAATG

CATTAAAATC

7TGTACAAGGA

'CGGTGCAGGA

r GAACTTATTA k AGTAACGGGC k. TCAAGAAATT

TAAACTAGCA

k. CGGCAATGGC r AAcGCCCAG r GTTGCTAGGA

GACACCACT

r GGTCGCAAAC A cATCAAACTA T CACGTGGAAT EF107-2 (SEQ ID NO:414)

MKRVN

WKRWLVVGLS CSLFADSVVG VTVLAETITG ATEQGVATSQ SSDEASQTTQ rI'EESQATVA SEAKTVPPQE TARIASRAIG YSSVEGREIP FFFVEEDGTL FDPDRITMAV N~LSTFSFYEE 207 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

KLQRTPLJEPT

TFYTGDTTQY

APGGVTYALT

QEDKXTIVTGE

DNDDLHVVYE

PTIDCIDMPA

DKTKYPVPNE

IYSGTAGGNY

YTVAXALPK I

TAELTLTGAV

GQNTKAFPVT

FGSLTAKD)TV

RNPYLRLNTS

KTSTVTLTAD

VTGP

TVNGGI(LLS I

PVPTVFANVG

YFYGDVGGPT

DIrFTQEGTL

E-AVM(TYTLP

SQLKKLAIPQ

VRRG IENPDN

NLSTPDGTIY

YQAGEKTYIF

DIIENGATMD

KEQWTTGAGV

RIKDLDQEIT

QANWSLTAQL

NTATAVVANQ

PTSPAFKYDT

GKVTNYVGAN

S SHQRRGTAC

PERYTGSDG(

AREALFGYVD

KVYTRPDDGT

IVSSLVGXXA

YYLENRRVTE

QGWFKGKTKP

YWEALLKNTG

S ITLDQPLPA.

SPIJCDGF 1ST

SQPKSATDSJ

QFTGSDVYQL

NNQNPSNIYG

AETELELTNE

PVYYYLTKRR

TYLFKGWYKG

EQGNLINPAI(

IVTYGPQEJS

YNLTQKSATR

HFVDESGAKI

ATLKTTTTPS

EAPLTTIXIK

JZGQLKMNhLLG PtEFGKLAl

PTTTRLLLGT

OFTFANIKLE

VSEVSFTIPK

KMPNKLTFGP

VTEKPENPAG

NAKPSTLETTI

FKLSATMGES

VEIPKYYQTI

YTARRSYWXW

TP PTGFTQGN

FTPTFNDEDD

PTATWAAG IG

TAVTGNPGQV

SGSKQQYGLK

AAAAS FTOYN

VPANQGMAGQ

EYQSLDIRPS

KKTFKYTVAT

GAl PAP EGYT

KTPSYAVTYD

DGATGEMTTF

S ISPTTAYTG

GPTKTLYSMS

QLVVDSENYV

MTAVYQEAI P

APNTIFVQGT

L'FADVEVTGN

KAADYYGNGT

QPTETRTPLG

QYQAAvTWNL EF107-3 (SEQ ID NO:415) GG AGCAAGGAGT AGCAACATCT

CAGTCGAGTG

GCTAGTGAAG

GGTTATTCTT

TTGrPTGATC

GAGAAATTAC

ATTCCAACGT

GGCGTTTCTG

AGTACGTTT

GGGGGCAAAG

GAAAAAATGC

ACGGCACCAG

ACTAGTTCGC

CGTGTCACGG

ACGCAGGATA

TTGCCTG-AAC

GGGAATGCGA

CATGACAATG

CCACCGAGAG

AAGTTTAAGC

TTTCCCACAA

CAAAAACTCT

AGTGTTGAAA

GGGGATAAAA

AACATrG=r.

CGCTATACTC

AGTATCTATI

TATTATI'ACJ

ATCACGCCAC

GTCTACACTC

TTCCAAGGC'.

AGTTrACA(

CCCACCGCG(

GATTACTGGC

AAGCCAACCC

ACGGGTCAk.

ACGAAGCGAG

cGAAAACAGT

CTGTGGAAG

CCGACCGAAT

AACGAACCCC

CACCAGCTTT

AAGTGTCGTT

ATACAGGAGA

TGACGAACTA

CCAATAAGCT

GAGGCGTrAC

ACCAAACACG

AAAAATIGA

AGAAAACCAT

GTTACACAGG

AACCTAGCAC

ACGATI'TGCA

AAGCTTW T

TAAGTGCGAC

TTGATGGAAT

*ACACACGCCC

CCTAAGTA

CCAAGTATCC

GTAGTTI'AGT

CCCGCCGTTC

CAGGAACTGC

TAGAAAATCC

CAACTGGCTI

TCGCAAAAGC

P~ GGTI'AAAGC :CAACmTTAI 3 AACTAACGT) 3AGGCGCTAC' 3CAACTTGGG( A ACACCAAAG( CCAGACGACG CAAACAACCG ACCGCCACAG

GAAACGGCAA

GCGCGAGATT

CCCTTITCT~

TACGATGGCG GTCAATCT CCTTGAGCCC ACCACTGTGA TAAATATGAT

ACAAATAACC

TACTATTCCT

CACTACGCAA

TGTGGGCGCC

GACG ITTGGT

GTATGCGCTG

AGGAACAGCG

GAATCCCGCA

TGTAACAGGG

CAGTGATGGG

GTGGAAACC

TGTGGTCTAT

CGGCTATGTT

CATGGGTGAA

CGATATGCCA

AGACGATGGG

TTACCAGACC

AGTACCAAAI

GGGAANCNCI1

TTACTGGANC

TGGGGGCAA(

GCGGGTCACr.

TACACAAGGj TT'TGCC5AAC CAAAACCAAi

TCATGAGGAI

SAACAGGTGC

1GAAGAACAC

-GGCTGGCAT

II7rCCTGT TATCCAGTc3C

AATGCGGAGA

CCTAAAAAGA

AC CTA~TT

GGTCCTGTGT

GGCGGGGCGA

GAGGATTTA

AAGACCTATT

ACCAAAACGC

GAAGAAGCAG

GATGAGCAAG

AGTGACGGAG

GCAA.GTCAAM

ACAATCGTA)

*ATTTCGATT1

GAAGTGCGCC

*GCGTATACJ

TGGGGCCCCJ

TATAATrTA' r' GAACATIT( k. AATCAGCTA( 3ATCTACCAAI 3 CCAGCAACA

"GACATGACC

C GTTGACATA A GGCGAAGCG C GGCGCACCC C ACCAAAGAA AAGAGTCACA G( GAATTGCTTC

TC

ITGTGGAGGA AC CCACGTTTTC

G'

ATGGCGGAAA

G'

AGAATCCAAG

T

AAAGCCTGGA C cAACGGTTrT T CGGAATTAGA

C

CGTITAAATAT

ATGGAGATGT

C

ATTATTATT

TTCCTGCGCC

CT'TTACCCA

TATTAAAGG

CTAGTTATGC C

TGATGAAAAC

GAAACTTGAT

CCACAGGGGA

TAAAGAAATT

CTTATGGCCCC

CACCAACTAC

GTGGCATCGA

7 TGACCCAAAA k CGAAGACACT C? CGACCCCTGA 3 TAGACGA;AG~ 3TGGTGGACAG cTGGTGAAAA r TAAAGACGAC S CTGTGTACCA A TCGAAAATGG- C CGTTlAACCAC A ACACGATATT .C AATGGACGAC 3CAACGGTC

CGAGCGATT

3ACGGGACG r TTATGAA

TTACTGTCT

P.ATA'FITAT

ATTCGACCA

GCGAACG'IT

TTAACCAAT

ACGGTAGCT

GGCGGTCCA

ACAAAGCGG

LGAAGGTTAT

LGAAGGCACC

.TGGTACAAA

;CTGACCTAT

TATACGTTG

L'AATCCCGCC

%ATGACGACT

kGCCATCCCG

GCAAGAAGTG-

rGCGTATACA

A.AACCCCGAC

AAGTGCCACA

ITrACTCAATG

TGGCACCATT

TGGCGCAAAA

TGAAA-ACTAT

AACCTATATC

AACGACCCCA

AGAAGCGATT

CGCCACAATG

CATTAAAATC

TGTACA-AGGA

CGGTGCAGGA

208 TABLE 1. Nucleofide and Amino Acid Seqeuences of E. faccalis Genes.

GTGTCCATCA

GGAACCGCCG

AACTTTWGGCA

ACGAGTCCTG

ATI'CAGGAA

ACTCGCAACC

CTATCGCAAC

ACGGCCGCTG

CGCAAGACCA

CAACAGTTCA

GA.AGTGCCAG

TTAGTGACTG

CGTTGGATCA

TTACAGGAAA

GTTAACTGC

ACGGCGACGG

GTAAGCAACA

CTTATTTACG

CAAAATCAGC

CTGCCAOCTT

GCACCGTGAC

CAGGCAGTGA

CCAACCAAGG

GCCCCT

GCCTTTACCA

TCCTGGTCAA

CAAACATACG

CTTTATTAGT

ATATGGTTTG

CCTGAATACT

CACAGACAGC

TACCGATAC

TTAACCGCC

CGTCTATCAG

TATGGCTGGC

GCTGGCGGTC

GT'TIAACCG

GTCCGTA 1TA

ACCCCGACAT

AAGAAGGCCG

AGCCA.AGCCA

TTGCCAACAA

AACCAACCAA

GACAATACCG

TTGGACTTCA

,CAACAATACC

AATTAAAAAT

CTGATGTTGA

AAGACTTAGA

TTGAT=tTGG

CAGATTACTA

ATTGGAGTTTI

CGA--CGCrT

CAGAAACCAG

CAACAGCGGT

CGTTTGCTAA

AAGCCGCCGT

GAACTTATTA

AGTAACGGGC

TCAAGAAATT

TAAACTAGCA

CGGCAATGGC

AACGGCCCAG

GTT-GCTAGGA

GACACCACT

CGTCGCAAAC

CATCAAACTA

CACGTGGAAT

EF107-4 (SEQ ID NJO:416) EQGVATSQ SSDEASQTTQ FTEESQATVA

B

SEAXTVPPQE

KLQRTPLEPT

TFYTGDTTQY

APGGVTYALT

QDKKTIVTGE

DNDDLHVVYE

PTIDGIDMPA

DKTKYPVPNE

IYSGTAGGNY

YTVAKALPKI

TAELTLTGAV

GQNTKAFPVT

FGSLTA(DTJ

RNPYLRLNTS

KTSTVTLTAD

VTGP

TARIASRAIG

TVNGGKLLS I

PVPTIVFANVG

YFYGDVGGPT

DFTFTQEGTL

EA'JM(TYTLP

SQLKIKLAI PQ

VRRGIE-NPDN

NLSTPDGTIY

YQAGEKTYIF

DIIENGATMD

KEQWTTGAGV

RIKDLDQEIT

QANWSLTAQL

NTATAVVANQ

YSSVEGREI P

PTSPAFKYDT

GKVTNYVGAN

SSHQRRGTAG

PERYTGSDGK

AREALFGYVD

KVYTRPDDGT

IVS SLVGXXA

YYLENRRVTE

QGWFKGKTKP

YWEALLKNTG

SITLDQPLPA

S PDGDGFI ST

SQPKSATDSL

QFTGSDVYQL

FFFVEEDGTL

NNQNPSNIYG

AETELELTNE

PVYYYLTKRR

TYLFKGWYKG

EQGNLINPAK

IVTYGPQEVS

YNLTQKSATR

HFVDESGAK I

ATLKTTTTPS

EAPLTTIKIK

GGQLKMNLLG

PTFDFGKLAI

PT±TTRLL-LGT

DFTFANIKLE

FDPIDR ITMAV

VSTEVSFTIPK

KMPNKLTFGP

VT:-KFENPAG

NAKPSTLETT

FKLSATMGES

VEIPKYYQTI

YTARRSYWXW

TPPTGFTQGN

FTPTFNDEDD

PTATWAAGIG

TAVTGNPGQV

SGSKQQYGLK

AAAASFTDYN

VPANQGMAGQ

NLSTFSFYEE

EYQSLDIRPS

KKTFKYTVAT

GAl PAPEGYT

KTPSYAVTYD

DGATGE.MTTF

SISPTTAYTG

GPTKTLYSMS

QLVVDSENYV

MTAVYQE.AIP

APNTI FVQGT

LTADVEVTGN

KAADYYGNGT

QPTETRTPLG

QYQAAVTWNL

EF108-1 (SEQ ID NO:417)

B

B.

TAATCGGTT

CAACGATTAG

ACGGCAG"TG

GAAGAGACGC

CAAGTAACAA

CAAGCAGAAG

AAGGCAACTr

CAATTAATAA

ACAGATGT

AACTTAAAGA

GAAGGAACTC

GCGAGTCTCT

CTAGCAGGTA

ACCAGTAAAG

GGTGTAAAGT

ATAGGTTATA

GAAAACTTCG

AAACAAACGG

ACCTACA.AG

GGCGGGAATC

CAACCATTGG

CGGAAACCGT

AAGCAAGTAG

CTGACACGAG

TGGAACAAC

ATTCATI'TTC

ATCCAGATAT

ATAATCGAAA

ATGTAATAJJ

AAGAGGTGTA

ACAATAGAGA

CTAGACCGCT

GAGACAATGT

TTACTAATTT

AGACTGTGAG

TAGATACAAG

TCATTAACAG

TTGGCACGAA

CTTGTGTAGT

GACGATTGAA

CGTGAAGGAA

TCAGGAAGAA

AGAAACACCA

TGCAGAAACT

TATACCAATT

GTGGACTAGT

GCCAGCAACT

CACAAACTTT

AGGTAAAAT

ATC'TFGACT

TTATACACCA

rTTGTATCGT

TAGCCCAATC

TGGCGCCAAA

TGATCCTTAC

ATCTTACCGA

TCTI'AGTAA

AGTAGTCCGA

GAAACAACGA

GCAACGAAAG

ATCATTCCTA

TATCAGT'ITG

ACGTATAGCT

ATGGTCAAG

TCTGTAGrTA

TCAATTCGCA

GATCTACTT

CAAAGTAGTG

CGGGAAACAT

ATAAATGACT

TATT!ACCATC

ATCACGCCAC

ACGTTCCAAC

TTCAAAGGCT

TI'AACGCCTT

CCGCCGAAAG

AAGCCAGTAC

AAGCGGAGAA

AACCAAAAAA

GATTTGTGAA

ATGCCAAAGG

GGAGTGCITC

TGCCACCAGG

TACCAAAATA

ATCCGTrACC

TAATTAGTGC

TTI'GGAGG

TTGATGTGAA

TGACCAACCG

CAAGTAATTI

AAAGTGGT

GGTACAAAGG

TTCTGGTGTG

TAGTGCCAAG

GGAAAATAGT

AGAAGA.ACCG

AATCAATATG

TGAATCAGGT

ATCATGGA6AG A6ACCGTAGGA

ACCGTCATAT

TIATGCATCA

TGCTAT1'GCA ATrGGCCTG

AGATCCTGCA

AGGTAATAAC

CCGTGTCACC

CACCCAAGGG

TTTAccCGAG GAAAAcCAAA GTACATAGAA AGAAGGGACG. ACATGA.AGCA AACTAAGTG 209 TABLE 1. Nucleotide and Amino Acid SeqeuenceS of E. faecalis Genes.

a a e *aaa..

a a .a.a a.

ACCGAGccT

GATTTGACGG

TTTGGCTTL'G

TATAATCTTA

ACAGATACAG

GTCAATACGT

ATCAATATTA

AATATAGATC

CTGTCGGGCC

ACGTTATI'TG

CCTGTTTACT

GCTAAAATCA

GCCTACACTT

TACAAGTTCA

GCGCCAAGTT

GAAACAGTTA

GCTTTCACAC

TTAAGAACCG

AGTATTAATA

CAACCAATCA

CAACTAAAA'I

TATAGATATCG

GAGGTTGACC

TT'PTCTAATC

GTTAACTATC

GTCTACAAAC

ATCACTGCAC

AAGTACACAC

TTCCA-AGGG'

ACGTTCAATC

CCAACAGCTA

ATCTGGACA.

AAAGGGCCC,

GGAGAAACG,

CCAAATGCC'

AAACCAAAC

GTGGATAAC

GGCTTCATC

CAALCACAGC

CGGAT'AAG

GCCACAGAC

TTACCAAM

AGCTTAAC;

AATGTTA]

GGTGTTAAJ

TGGCCACCAC

TGGTCTATGA

TGGATGAAGC

cTTrTAA-ATGA

CGACACTGAA

TTTATGGCGC

CCAAATCAGA

AAGTAGAAAT

AAACGTTGG

GGACAGAGTC

ATTATAGA

CACCCCCAAC

TCAAACAAGC

AAGGTTGGTA

ATCAAGTGAC

CGACAGTGTA

CGGCGTTAAC

ATTTATATGA

ATGGTAGTAIl

GTGCTACCAP

ATGTTGACAC

TGTACACGAJ

TTAGTTCAGIt

CAAATAATAC

TCTCGTAAM

TCACTCGCA2 CAACAGGCr

CGGCAAAAG(

r' GGTATAAAG( 3 CGACC TG, D, GTGTCACAT k. CAACGATCA P, ATTGGTCAG A CAAAATCAA G TTCCTATCG A CTGTTTITGA T TCGTGAGAA A GTCTGCCAP T TGAAACAAG- A AAACGCAAC A GCTTGCCTI

ACAATCAAC

iG CCAACAACI .C AGTTGGAC' G GGCAACAA

TAAAACACCT

GGAG~rTTCA

GACGAACAAA

AAATAATAAA

AAAC'LTGTCC

GAGTGACAT'r

TGGCAAA.ACC

GTCACACATG

C CTTAATGCT

TGGCATCGAT

AAACCGCAAA

AGGTTTCACC

AGGCACCTTA

CAAAGGCAAG

CTACGATGAC

TCCATCAGTC

TTTrAGTGGT

CGTGACCTCA

GCCATTGTCC

CAGATTACAG

CATTCAATTP

CAATAGCTCP

ATCTCTTAAC

ACTTTTTAC

C' GT1'TTTTJ

ACAAGTCACC

r CACCCA-AGG'.

TTTACCAGC(

S GAAAACCAA( AL TGGCAATGA, T AACTCGACC.

C GAATACTAG C TGGTCTGAC .T CCCAGTAAA G CAAAA.AGT A AGCAGAAGTl ,T TCGTCCAAA 6C CTTCGAC'TI ;C CGCGGA TP .C CAATTGGAC C AGCGACCCC :C AACCGAGT kC AGCAACGAC rr CACCTICAJ rA CAAGGCCG(

AGCTATAAAG

GGGTACGAGC

TTAATTGCCC

AAAACAGTAA

GTGCCTGTCA

ACGTTTACAT

GACCCAGCTT

CCTGTGACCA

TTAGCCGATC

GACCCAGTCA

GTFCACCGAGA

CA&tAAAA

CCAGACACTT

TCCATACTCA

AATGATGATT

GATATGAACT

AAGTACTATG

AAAAATAATG

CAAGAATTI'I

TTTAATGTTC

GACACAGCTC

CTGG'rT71rCC TTGCTMAT J

ACGCATTTA(

LAACGCCAAA(

GAAAACTI'C(

r~ AACCAAGTA( 3ACGTATACT 3CCAAGTACrG

GATATGACC

A AAAGAAGTG C AAAGCACCC G ATCCCGACC T AGTACACTT T TCAGM1CCI T GTAGTATI XT GATCAAGAP ,c GGCCAAGTIC LC TACGGTAAC ;C TTAACAGC:C ,c TTATTA'I1 M' AAAAATACC ;T ATTATTGC( k.T AATGTCAA :A GTTACATG( TCACGTATGA TG TGCCTGCTTC

GA

CCGACCAAGT

GC

TGAGCAGTA-A C9 ACTATTTrTGA

A(

TGCCCAAACG

G

TTCCTCFI'CC

T~

CTTATAACAA

G~

AACCTGAATT

T

ATI'AITATAC k AC LTCGTAGA

C.

AA-ACGGTGAT

T

ACACAACG

C

ACACATTGACA

TGAATGTGGT

G

TTGTGAATGA CGCAAAGTAC G GTAATGGGCA

A~

TGAAAAAATA ATAA.ATTAG'C

C

AAAGTAGCAA

I

ACCCAAATGT P' TTGATTCAGA 3 GATATAGTGG

:CTGCGGATAA

3TGGATCTCAA Z: CAATGAACAG A CAGGTGGCAA

TGAACAAAAC

G CCATGTATAA A Tr'GATACGAA T TACAAAATCT T TTATGGAAGT ~T GGACAGAGGG T TCACAACTCG 'C GTGGTATTAA LG TAGTCACACC ;G GCGTTGCAGG 'G GCACACGGAA 'C AACTGTCACA kG GGGCGGCGCC 'G TCGGTACCAC :A ACAAGCAATr AC TTGAAGTGCC

ACCTAGTTAC

ACAATCAT

~CCAATCAA

:AGATGAAG

~TAACGGG

AATATCGC

L'ACAAATCA

U.AAATCTAT

I'TGAAACAG

rATACGAAA

ATGAGTGGC

ACCA.ACGGC

ACAAGCGAC

GGTAAGACC

ACTACCAAA

TATGAAGAA

AAAGGCGGG

AGTGCGTAC

~TATACGGTA

.AATAATGGA

:ATCGACCAA

~CTGAAATCC

WGCACCAGCA

LGCCACCTAT

:ACAC-CAGG-A

GTCAAAACTT

CGGTGCCAA

rAACACCTTC

AGTCTATACG

AACAACTCCA

GGAAGAAATA

TACCAATGTA

CACCTTGAAA

GACACCAGAA

GGTTCCTI'TA

CGCAACAGG

AGATACTACA

AACGACCGAA

AACTAAGCAA

TCCGTATCTG

ACcAAAATCA

TGT-CTCTAGC

GAGTGCCATT

CACAGGTAGT

AGCCAATCAA

AGGTCCTTAA

EF108-2 (SE.Q ID NO:418) MKQTKWQ RLATIGLCSS LVINAFSGVT AVAETVTIES

SPTAESSAKE

ETQASSVKEE TTKASTENSQ V'ITDTSQEFEA TKEA.EKEEPQ

AEVEQAETPI

ATYSFSAETY QFGFVNESGQ LINPDI IP IT YSYAI<GSWKT

DGYNRKWTSM

LKNVIMPATS VVMPPGPSYE GTQEVYTNFS IRIPKCYYASA

SLYNREGKID

AGTRPLSLTQ SSVISALALT SKGDNVYTPR ETFFGGDPAG

VKFTNFLYRI

GYK'IVSSPIY YHLTNRRVTE NFVDTSGAIKI TPPS4FTQGK

QTVINSDPYT

IPKPKINM'K

VQGSASTVGN

STYPLPAIAL

NDFDVYKGNNI

FQQSGFLPET

210 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

YKVGTKSYRF

GFVDEATNKL

NTFYGASDIT

SGQTFGFNAL

KITPPTGFTQ

PSYQVTYDDN

RTDLYDVTSK

LKYVDS IQLD

SNANNRLFYT

TAPTGFTQGN

FNATFDGNDD

GPNWSAGLTI

PNTVLKAEVV

HSLJKQAADYY

TNYNQPTELK

KGWYKGKTKT

IAPDQVQM(Y

FTLPXRYKS I

ADQPEFYTKT

GKKTVITSDA

DDLNVVYEEE

NNGNGQYTVS

TAQSSNLKSY

ELGYSGTPGV

QVPMNhSNTFK MTAMYXEE IP

PTFMEVTPEG

VFGC IKO)STV

GNGTRNPYLR

NTVGTTSAIS

EPLATI'XTPS

NLTLNENNKK

NITKSDGKTD

LFGTESGIDD

YTFKQAGTLP

TVTTVYPSVD

INNGSMPLSQ

RYVYTNNSSL

NYLLVMFLFN

YTAAKALPAT

TASVTLTRPK

ETTKSIPVNS

DNFVRIRPND

IKKTQPNWSL

LTANNTATS I

YKVTY)DNIDD

TVMSSNLTGT

PAFPLPKIYN

PVNYYTMSGP

DTYTTGGKTY

NNFVNEKGGA

ELLKKYNNGQ

VFDPNVAPAE

AKPADKSKIJV

YTTGGKVYTF

EVIDTNTNVI

TLWTEGVPLP

QEVVTPTTEG

TAQLSQPKSA

IANKQFTGSN

LTVVYEEFSG

DTATLI(NLSV

IDQVEMSHMP

VYYYLENRKV

KFKGWYKGKS

FTPALTFSGK

P ISATNRLQF

VDLSSESLNL

YKVTRI(QVTE

QGWYKGKTKP

WTTTITNTSK

NAVP IG1GVS

FISVPTFDFG

TOSL.TATRL

VYQLDFTFNN

YELPASTNQF

PVNYFEQYRV

VTTYNKLKQL

TENFVDTNGA

ILNTLTTTKA

YYAQSTSAYL

NVDKLAIDQQ

LNFOSDGTYF

NFVDVNGAK I STrLNKTTTPT

APLQNLTLKK

VAFTTRATGK

QVGVACTKQQ

LLGAAPVSSF

VKLEVPANQG

VKGQQYKAAV T.JNLVTGP EF108-3 (SEQ ID NQ:419) 9.

9* 9 9 9 9, 9 .9.9 9 9*9~ 9* S 9* 9* CGT GACGA PrGAA AGTAGTCCGA CCGCCGAAAG TAGTGCCAAG

GAAGAGACGC

CAAGTAACAA

CAAGCAGAAG

AAGGCAACTT

CAATTAATAA

ACAGATGGTT

AACTTAAAGA

GAAGGAACTC

GCGAGTCTCT

CTAGCAGGTA

ACCAGTAAAG

GGTGTAAAGT

ATAGGTTATA

GAAAACrI'CG

AAACAAACGG

ACCTACAAAG

ACCGAGCC T

GATTTCACCG

TTTGGCTTTG

TATAATCTTA

ACAGATACAG

GTCAATACGT

ATCAATATTA

AATATAGATC

CTGTCGGGCC

ACGTTATTTG

CCTGT'ITAC'I

GCTAAAATCA

GCCTACACTI

TACAAGTTCPA

GCGCCAAG PI

GAAACAGTTP

GCITTCACAC

TTAAGAACCC

AAGCAAGTAG C CTGACACGAG T1 TGGAACAAGC A

ATTCA=TTC

ATCCAGATAT T1 ATAATCGAA-A C ATGTAATAAT C

AAGAGGTGTAC

ACAATAGAGA I

CTAGACCGCT

GAGACAATGT

TrACTAATT

AGACTGTGAG

TAGATACAAG

TCATTAACAG

TTGGCACGAA

TGGCCACCAC

TGGTCTATGA

TGGATGAAGC

CTTTAAATGA

CGACACTGAA

TTrATGGCGC

CCAAATCAGA

AAGTAGAAAT

AAACGTTTIGG

GGACAGAGTC

ATTATTTAGA

*CACCGCCAAC

*TCAAACAAGC

AAGGTTGGTA

ATCAACTGAC

LCGACAGTGTA

CGGCGTTAAC

ATTTATATCA

GTGAAGGAA C CAGGAAGAA C GAAACACCA I ,GCAGAAACT I

~ATACCAA

;TGGACTAGT

;CCAGCAACT

'ACAAACT

~GGTAAAA IT kTCTTTGACT

LTATACACCA

I=rTATCGT

TAGCCCAATC

TGGCGCCAAA

TGATCCTTAC

ATCTTACCGA

TAAAACACCT

GGAGT=CA

GACGAACAAA

AAATAATAAA

AAACTTGTCC

GAGTGACATT

TGGCAAAACC

GTCACACATG

CTITAATGCT

TGGCATCGAT

AAACCGCAAA

AGGT'ITCACC

AGGCACCTTA

CAAAGGCAAG

CTACCATGAC

TCCATCAGTC

TI'TAGTGGT

CGTGACCTCA

,AAACAACGA

CA.ACGAAAG

~TCAT1'CCTA EATCAGTTTG C kCGTATAGCT k.TGG'PTCAAG E'cTGTAGTTA r'cAATTcGcA

"ATTCTACTT

AAAGTAGTG

CGGGAAACAT

A~TAAATGACT

TATTACCATC

ATCACGCCAC

ACGTTCCAAC

TTCAAAGGCT

AGCTATAA.AG

GGGTACGAGC

'ITAATTGCCC

AAAACAGTAA

GTGCCTGTCA

ACGTTTACAT

GACCCAGCTT

CCTGTGACCA

TTAGCCGATC

GACCCAGTCA

GTCACCGAGA

CAAGGTAAAA

CCAGACAC'T

TCCATACTCA

AATtGATGAT1'

GATATGAACT

AAGTACTATG

AAAAATAATG

LAGCCAGTAC G LAGCGGAGAA A~

LACCAAAAAA

;ATTTGTGAA

kTGCCAAAGG P

;GAGTCCTTC

EGCCACCAGG TACCAAAA'TA 9 kTCCGTTACC TA-A TAGTGC rTTTTrGGAGG

TTGATGTGAA

TGACCAACCG

CAAGTAAT

AAAGTGGTFI'

GGTACAAAGG

TCACGTATGA

TGCCTGCTTC

CCGACCAAGT

TGAGCAGTAA

ACTATTI'GA

TGCCCAAACG

TTCCTCTTCC

CTTATAACAA

AACCTGAATT

ATTA7rATAC

ACTICGTAGA

AAACGGTGAT

ACACAACAGG

ACACATTGAC

TGAATGTGGT

TTGTGAATGA

CGCAAAGTAC

GTAATGGGCA

GAAAATAGT

.CAAGAACCG

LATCAATATG

,GAATCAGGT

LTCATGGAAG

LCCGTAGGA

~CCGTCATAT

~TATGCATCA

rGCTATTGCA kTITGGCGCTG kGATCCTGCA kGGTAATAAC

CCGTGTCACC

CAccCAAGGG rTTACCCGAG

CAAA.ACCAAA

TGACAATGAT

GACCAATCAA

GCAGATGAAG

CTTAACGGGG

ACAATATCGC

GTACAAATCA

TAAAATCTAT

GT'GrAAACAG

TTATACGA;A

AATGAGTGGC

CACCAACGGC

TACAAGCGAC

CGGTAAGACC

AACTACCAAA

GTATGAAGAA

AAAAGGCGGG

GAGTGCGTAC

ATATACGGTA

211 TABLE 1. Nucleotidc and Amino Acid Seqeuences of E. faecalis Genes.

AGTATTAATA ATGGTAGTAT CAACCAATCA GTGCTACCAA CAACTAAAAT ATG7r'IACAG TATAGATATG TGTACACGAA GAGGFI'GACC TTAGTTCAGA T'TTTCTAATG CAAATA.ATAG GT~AACTATC TTCTCGTAAT GTCTACAAAG TCACTCGCAA ATCACTGCAC CAACAGGCTT AACTACACAG CGGCAAAAGC TTCCAAGGGT GGTATAAAGG ACGTTCAATG CGACCTTTIGA CCAACAGCTA GTGTCACATT ATCTGGACAA CAACGATCAC AAAGGGCCCA ATTGGTCAGC GGAGAA.ACGA CAAAATCAAT CCAAATGCCG TTCCTATCGG AAACCAAACA CTGTI'GAA GTGGATAACT TCGTCAGAAT **GGCTTCATCA

GTGTGCCAAC

CAACACAGCT TGAAACAAGC **CGGATTAAGA

AAACGCAACC

GCGACAGACA GCTTGCCTAC VTTACCAATTI ACAATCAACC *AGCTTAACAG CCAACAACAC *AATGTTTATC

AGTTGGACTI]

GGTGTTAAAG GGCAACAAT;

GCCATTGTCC

CAGATTACAG

CATTCAATTA

CAATAGCTCA

ATCTCTTAAC

ACTT'TTTTAC

GTTTCTTTTT

ACAAGTCACC

CACCCAAGGT

TTTACCAGCG

GAAAACCAAG

TGGCAATGAC

AACTCG-ACCA

GAATACTAC

TGGTCTGACG

CCCAGTAAAT

CAAAAAAGTr

AGCAGAAGTE

TCOTCCAA-AE

CTLTCGACTTC

CGCGGATTAC

CAATTGGAGC

AGCGACCCGC

AACCGAGTTC

AGCAACGAG

CACCTTCAA'

CAAGGCCGC

CA.AGAATTAT

TTTAATGTTG

GACACAGCTC

CTGGTI'MTCG

TTGCTPAATT

ACGCATTTAG

AACCCCAAAC

GAAAACTTCG

A.ACCAAGTAC

ACGTATACTA

.CCAAGTACGT

GAPfitTGACCG

AAAGAACTGA

AAAGCACCCT

ATCCCGACCT

AGTACACTT

*TCAGTTGCMF

GTAGTATTTC

GATCAAGAAC

GGCCAAGTGCC

TACGGTAACC

TTAACAGCGC

TTATTATTA(

AAAAATACG(

C' ATTATTGCC r' AATGTCAAA( k GTTACATGG, TGAAAAAATA T ATAAATTAGC C AAAGTAGCAA I

ACCCAAATGT

TTGATTCAGA

GATATAGTGG

CTGCGGATAA

TGGATGTCAA

CAATGAACAG

CAGGTGGCAA

TGAACAAAAC

CCATGTATAA

MrATACGAA

TACAAAATCT

TTATGGAAGT

GGACAGAGGG

TCACAACTCG

GTGGTATTAA

TAGTCACACC

GCGTTGCAGG

GCACACGGAA

AACTGTCACA

GGGCGGCCCC

TCGGTACCAC

k. ACAAGCAATT

-TTGAAGTCC

k~ ACCTAGTTAC

~AATPLATGGA

:ATCGACCALA

~CTGAAATCC

=GACCAGCA

LGGCACCTAT

:ACACCAGGA

'TCAAAACTT

:GGTGCCAAA

rA.ACACCTTC

AGTCTATACG

AACAACTCCA

GGAAGAXATA

TAC ATGTA

CACCTTGAAA

GACACCAGAA

GGTTCCT ITA

CGCA.ACAGGG

AGATAGTACA

AACGACCGAA

AACTAAGCAA

TCCGTATCTG

ACCAAAATCA

TGTCTCTAGC

GAGTGCCATT

CACAGGTAGT

AGCCAATCAA

AG

EF108-4 (SEQ ID 110:420) VTIES SPTAESSAKE a -TQAS SVKEE

ATYSFSAETY

LKNVIMPATS

AGTRPLSLTQ

GYKIVSSP IY

YKVGTKSYRF

GFVDE-ATNKL

NTFYGASDIT

SGQTFGFNAL

KITPPTGFTQ

PSYQVTYDDN

RTDLYDVTSK

LKYVDS IQLD

SNANNRLFYT

TAPTGFTQGN

FNATFDGNDD

GPNWSAGLTI

PNTVLKAEV

HSLKQAADYY

TNYNQPTEL<

TTYKASTENSQ

QFGFVNESGQ

VVI4PPGPSYE

SSVISALALT

YHLTNRRVTE

KGWYKGKTKT

IAPOQVQMXY

FTLPKRYKS I

ADQPEFYTKT

GKKTVITSDA

DDLNVVYEEE

NNGNGQYTVS

TAQSSNLKSY

HLGYSGTPGV

QVP4I'SNTFK MTAMYKEEI P

PTFMEVJTPEO

VFGG IKDSTU

GNGTRNPYLP

*N'IVG'TSAIS

VTTDTSQEEA

LINPDI IP IT

GTQEVYTNFS

SKGDNVYTPR

NFVDTSGAKI

EPLATTKTPS

NLTLNENNKK

NITKSDGKTD

LFGTESGIDD

YTFKQAGTLP

TVTTVYPSVD

INNGSMPLSQ

RYVYTNNSSLi

NYLLVMFLFN

YTAAKALPAT

TASVTLTRPK

ETrKSIPVNS

DNFVRIRPNV

IKKTQPNWSL

LTANNTATSI

TKEAEKEEPQ

YSYAKGSWKT

IRIPKYYASA

ETFFGGDPAG

TPPSNFTQGK

YKVTrYDDNDD

TVMSSNLTGT

PAFPLPKIYN

PVNYYTMSGP

DTYTTGGKTY

MNFVNEKGGA

ELLKKYNNGQ

VFDPNAPAE

A.KPADKSKLV

YTTGGKVYTF

EVIDTNrNVI

TLWTEGVPLP

QEVVTPTTEG

TAQLSQPKSA

IANKQFTGSN

AEVEQAETPI

DGYNRKWTSM

SLYNREGKID

VKFTNFLYRI

QIVINSUPYT

LTVVYE-EFSG

DTATLKNLSV

IDQVEMSHMP

VYYYLE-NRKV

KFKGVIYKGKS

FTPALTFSGK

PISATNRLQF

VDEJSSESLNL

YKVTRKQVTE

QGWdYKGKTKP WTM ITNTSK NM?? IGKKVS Fl SVPTFDFG

TDSLPTATRL

IVYQLDFTFNN

IPKPKKIOM~K

VQGSASTVGN

STYPLPAIAL

NDFDVKGNNI

FQQSGFIPET

YELPASTNQF

PVNYFEQYRV

rTytNKL.KQL

TENFVDTNGA

ILNTLTT KA

YYAQSTSAYL

NVDKLAIDQQ

LNFDSDGTYF

NFVDVNGAX

I

STLNKTTTPT

APLQNLTLKK

VAFTTRATGK

QVGVAGTKQQ

LLGAAP VS SF

VKLEVPANQG

VKGQQYKAAV TWNLVT EF109-1 (SEQ ID NO:421) 212 TABLE 1. Nucleotide and Amnino Acid Seqeuences of E. faecalis Genes.

AGGAGTAAAT

GGA=TCTCG

GAAACGGTGG

GAAATGAACC

ACCTCCGAT

NATAGTAGCG

TAG

TAATGAAAAA

TTACCCCTAT

CTTCAGAAAC

CAAGCATCAT

CCACCACTGA

ACGTACTGAA

AAGTGTTATA

TTCTGCTTAC

ATCTCTAACG

AAATTCTCAA

AG=ITCTACA

ACTAC'FTTGG

ACTAGTTCTA

GC TTGGAAC

GAGCGACAAA

GAGGAAACAG

TCAGAAGTAA

NAACATCACN

TGTT-AGCGGT

GCTCTAAGGG

TGAGTAGCGG

AAACAACGTC

CAACTGTTAA

AAGTAATGAG

TTTGTTGTCG

AACTACTGAA

TGTCACTGAA

CACTTCCTCA

TGATACAGAA

GACACACCTA

E.F109-2 (SEQ ID NO:422)- 14KKSVI TSSMLAVLLS GFLVTPISAY ALERSKGTTE ETVASE TSLT ERQMSSGVTE EMNPSIINSQ EETETTSTSS TSDSTTEVST SEVTTVNDTE XSSIDVLKLLW XHHXVMRTHL EF109-3 (SEQ ID NO:423) GGAAC GCTCTAAGGG AACTACTGAA GAAACGGTGG CTTCAGAAAC ATCTCTAACG GAGCGACAAA TGAGTAGCGG TGTCACTGAA GAAATGAACC CAAGCATCAT AAATTCTCAA GAGGAAACAG AAACA.ACGTC CACTTCCTCA ACCTCCGATT CCACCACTGA AGTTTCTACA TCAG EF109-4 (SEQ ID NO:424) ERSKGTTE ETVASETSLT ERQMSSGVTE EMNPSIINSQ EET=E'FTSTSS TSDSTTEVST S EF110-1 (SEQ ID NO:425)

C

TAAATAAAAA

AGTGCTGGTT

AATGCAGAAG

GACCCTGAGG

GGA.AGA.ATCA

GTTGGAACCA

GTATTAAATC

ACACCAT ITG

GTAGTGACTG

ACGCCATI'G

CCAGGTGAGA

ACAGACTTAG

TCACCAATCT

CAAACAGGAA

GTGAATGAAG

TGGATAAGGA

'rrwCTTTCT

AGTATATCGT

ACAGAAGACA

TTTCCCCTGC

ATACAAT

CGAATGCCAA

GCAAATTCAA

TCGGCAAACA

~TTGAAAAA

AAAACCACAC

AAAATCCATT

ATAATGATCA

ATCATGGTCA

AAGAAAkATAA

GTGGCATAAT

GATTTTAGTA

TCCTGCCGAA

AGAAGTGGCA

CAGTAAACCA

CACCAATAAT

AGATGATGCT

AGTGATTGAT

AAACGATCGT

GT'ITGAATCT

ACAATGGTCT

ACTAIFTAT

GG ITGAAGTA

AAGACTAAAT

ACGITTTATCC

CTTATGAAAA

ACTI'TGATCG

AGTCATTCAC

GATACAkACCG

GGCTATATTT

CA.TGTGGCTG

TGGTTTTATC

GTAGCTrTTT

CCAGATGGCC

TCAGATACCC

CATGAAAATG

GATATCGATA

GTTGGTGTTC

GAAGrTGAATT

GCTGTGCCAG

AGTTCTCCAT

CCGGF=AG

GACAAAAAAG

AAGCGCCFI

CTTTAGGAAC

AAAGTTTAA

CAGGTCGAGA

CCCCGAATGC

CAGAGTTGGG

ATGTCACAAT

ATT'GTTTAC

CAACCGGCGG

ATTCCAATGG

ATAACTTTAT

CAGCGTAA

ACGAAAAAT

C'ITTCTGCA

ATCGTTACTG,

TGCGTCAATC

AGGCTI'TGTT

GAATGCCAAA

TGGCAGTGCG

GGATATTGCG

AGAAATTTA

ATCAGGCTAT

ATCTAACTTT

TcAATCTGT

CGGCATTAAG

TGTTAATCGA

EF110-2 (SEQ ID NO:426)

MKKFSIRKIS

PEDRRQEVAD

LNPNAKDDAW

PFVLKKFESS

P IYNDQVEVV AGFLFL ILVT TTEAkPFAS IG

FYPGRDGSAT

DTHVTISGYP

GVHSNGGIKQ

LIAGFSLSAN

RIISPASKPG

PFGKFKVIDV

GEI(NHTQWSH

TGNHGQRLNE

AEEYIVPAES HSRQKRSLLD

YISLGTGFVV

AFsPNADIAV

ENDLFTSNFT

VNYNFIVNRV

GTMTIVTNNH VAESFKNAKV VTVGKQNDRP DGPELGEILT DLENPLLFYD IDTTGGQSGS NEEENKRLSA VPAA EF110-3 (SEQ ID NO:427) AG AGTATATCGT TCC LGCCGA-A AGTCA'FTCAC GACAAAAAAG ATCGTTACTG 213 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

GACCCTGAGG

GGAAGAATCA

GTTGGAACCA

GTAT TAAATC

ACACCATTTG

GTAGTGACTG

ACGCCATTTG

CCAGGTGAGA

ACAGACTTAG

TCACCAATCT

CAAACAGGAA

GTGAATGAAG

ACAGAAGACA

TTCCCCTGC

ATACAATTGT

CGAATGCCAA

GCAAkATTCAA

TC'GGCAAACA

TTTTGAAAAA

AAAACCACAC

AAAATCCATI'

ATAATGATCA

ATCATGGTCA

AAGAAAATAA

AGAAGTGGCA

CAGTAAACCA

CACCAATAAT

AGATGATGCT

AGTGATTGAT

AA.ACGATCGT

GTTTGAATCT

ACAATGGTCT

ACTAITI AT

GGTTGAAGTA

AAGACTAAAT

ACGTTTATCC

GATACAACCC

G4GCTATATTT

CATGTGGCTG

TGGT=TATC

GTAGCTITTTT

CCAGATGGCC

TCAGATACCC

CATGAAAATG

GATATCGATA

GTTGGTGIC

GAAGTGAANr GCtGtGCCAG AAGCSCCTTT TGCGTCAATC C'I TAGGAAC AGGCTTTGTT AAAGTTTTAA GALATGCCAAA CAGGTCGAGA TGGCAGTGCG CCCCGAATGC GGATATTGCG CAGAGTTOGG AGAATTTA ATGTCACAAT ATCAGGCTAT ATTTGTTTAC ATCTAACTTT CAACCGGCG-G TCAATCTGGT ATTCCAATG-G cG~cATTAAG ATAACTTITAT

TGTTAATCGA

CAGCGT

EFIJQ-4 (SEQ ID NO:428) EY IVPAES HSRQKRSLLD PEDRRQEVAD TrEAPFAS IG LNPNAKDDAW FYPGRDGSAT PFVLKKFESS DT]-VTISGYP PIYNDQVEVV GVHSNGGIKQ RI ISPASKPG

PFGKFKVIDV

GEKNHTQWSH

TGNHGQRLNE

YISLGTGFVV

AFSPNADIAV

ENDLFTSNFT

VNYNFIVNRV

GTNTIVTNNH

VTVGKQNDRP

DLENPLLFYID

NEEENKRLSA

vAESFKNAKV

DGPELGEILT

IDTTGGQSGS

VPAA

a a.

a a a a.

a a a EF111-l (SEQ ID NO:429) a a a

TGATCAATAC

AAAAAGGGAT

CTATCATTTT

TGGTTAGCGT

AGTGTTGAAA

ACCGAACGGA

GCA I'GCATG

GGGCCATTGA

ACGGTCAAAA

CTGTCTTATT

TTAGC'TITAA

GGTGAAGAC

GAGGGAACGG

GTGAATTI'TG

ACGATI'CATC

GCTGAACCAA

ACTT?1'ACAA

GCCGCGCGCT

GATCAAAAGA

ACGATTAATA

TCCATGTCTI

GCA.ACGGCTC

CAA.ATrcAAP

TTTTACAATC

AAACCACCAI

TTTTTAAAAC

GACCACAAT;

AAAGACGACI

CTGTTATG)-

AAGGTGTGG(

TAG

ACTTCGATAC

ACACTCCATT

ATAGGAGGGG

TAGGAATCAC

GAACAAAAGT

CGAATGGCGT

GCTACTATTT

TTATTGCGGA

ATAAAAAAAC

ATCCTGGGCG

TTTTTGTC-AG

CCTTGTCACT

AAACCTTAGA

GTGAAGTCAG

ATGCGGATAA

TIGAATTGAA

AAGAAGAAGP

ATTTCAAAGP

AAACAGCAG;

CCAATTGGCC

ATAAATGGT]

ATTT1'AATCC

AAGATGATAC

AAGACAGTG(

TGCCTGCAT(

AAATGTATC(

AAATGGGA!

kAGGATCAAA' k AGCAGCCGCI 3CAAAGGCGA

GGTCGCTTTT

TGGCATAGTC

'IT=ATCAAG

CGTAGTCAGC

TGATGAAFI'T

ATACCATACC

ACCTGAAAAA

AGAATATCCA

GGGAGAA-ACC

CCTAGAGCAA

CAATCGAACG

TGGAGCAAGC

TAT'MGCACT

AGAAACGTGG

AGTTTCAACA

GCCTAAACAA

GGCAAAGC-AA

GAACAAGCAA

ATTTCCTGAA

AAGTGCGGCP

TATTGGTATC.

TGACTTAGCI

CGTACGTCC;

GCGTGGTGGI

G GCAGTTTGC

CAAACT

r AGCAGAATA! r CATAAAGA! 7. TGGGAAAGT(

TTTCTAGAGA

CF1'GCTGATA A

GGTTTATCAA

TGTT'ITGCGIT I GCAAATGTT 'I

AAI'ATTTTAI

AGCAATAAAG

GTAAACTTGG

TATGATTTAA(

ACCTATGAAT

GCGCTTATCC

TGGACAGGTG

CGTTTAACTG

AATTAITTG

AAAATTGATA

ACGTACAACA

CAACAACAAG

AGATGGCAAG

TATCAAAATG

GGTGCCTTrA

*TGGGCTTGGG

AAAAATAATA

CAAGATGCAG

GAAGGTGGCA

CATATT1TATC

GCTTATCATA

r' GGAAGCATGG r AAAAATGGCC

-GCATGCATAA

LAGTTGAATC T ~TAAATCAGT G

LAAAGAAACGC

~AAGCAGA

~AGATGTGAG Ti ~TAATTTTT'C TI GCCTACTCG1

:GGCAAGTTT

3CCA-AAGCAA C rAGACGA'ITT I

PLAACGACACT

CGGTCTrGA

CTAAAGACAA

CTACGAAAGA

ATCGGAATTA

CCTATACGAC

CACCCCAATA

GATATCTAGA

CGCTACTCAA

ACCATCACG

A'ITCGTGGAA

TGCGGGCCTT

GAGCGATCAT

ACTGGAATGA

AAGAAACCAA

A'TCGTGCTA

TCAGTGATGC

ACCTAAAGTG

CGCTACACCO

AAACAAAAAT

TTCAATAAT

TATAAAGCG

GTGTCTACT

,GTGCAAGCA

'GCATCACCA

~CATTTAGCT

'CCTTTTGC

LAACAAATTA

CGCATG-AC

k.ACGATCAT

L'GAAAACACT

:AAAATTCAA

TGACATTCAA

CACAAAATAT

rACAGCAAC

AGAAAGCTAT

TACCAAAAAT

TAAAACGTTT

ATCGATTGCAA

GATTCTTCCG

AGCGGATGTA

GTTTGA'ITAT

TGATGCTGTC

ACCAAATTCT

AGATAAGGAA

TACCAACAGA

TCACTCCCAA

GATGATGATG

TTTCACAAAG

AALAGGAAAAG

GTTGGAGTTA AAGTTTTTGA 214 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

EF111-2 (SEQ ID NO:430) DM(G LSKKKRVSTW

LALGITVVSC

WHGYYLPEKS

SYY PGRLEQT

GTETLDIGTR

EP IELKPI(QT

QKKTAEFPEY

TADFNPELAK

PPLAAWAVWH

DDKDQI IKDK

FALSREVQAS

NKELLGGFAG

YELDOLTIHL

LTAKONDIQV

VNTYTTESYT

QNALVKS lET

NNMRALFDYQ

IYQETKDKEF

NGI4LKWMMML

VERTKVDEFA

PLIIAEEYPV

ALIFVSNRTA

NFGEVRE.TWN

FTKEEEAKEQ

INTNWRSAAG

IQKDDTVRPQ

LKE4YPKLVA

LLKQPRGKVA

NVLOVSASPT

NLAASLNK:LT

LI QTTLENTG YFATK[YrKYT

QQAPE-YTKNA

AFKHDGIVPS

DAGAI IDAVF YI4RWWTNRD

WITLHGLTKK

E-RTNGVYDTN

VK<NKKTGETY

7-EPLSLGASW

IHHADKVSTK

ARYFKENKQR

MSYKWFIGMW

YNQDSARGG7E HNXNG IAEYG

VAAATLELK

YFNNFSDLGA

DLSQSNRMDL

TGAVFDKIQE

IDNRNYTATA

WQGYLDKTFD

AWDSWKADVA

GGNWNERNSK

SMVSDAHWQK

FLKTK IKEK EF111-3 (SEQ ID NO:431) S S

SS

5 5 0* 5* S S S 5

S.

S S

S

S

S

5

S

55 5 S S

S.

TCYATGAATE

5

I

ACCGAACGGA

GCATGGCATG

GGGCCATTGA

ACGGTCAAAA

CTGTCTTATT

TTAGC=IAA

GGTGAAGAGC

GAGGGAACGG

GTGAATTI'TG

ACGATTCATC

GCTGAACCAA

ACTTTACAA

GCGGCGCGCT

GATCAAAAGA

ACGATTAATA

TCCATGTCTT

GCAACGGCTG

CAAATTCAAA

TTTTACA-ATC

AAACCACCA'I

TrTTTAAAAC

GACCACAATP

AAAGACGAC;

CTGTTATTG;

AAGGTGTGGC

GCAAATGT

CGAATGGCGT

GCTACTATT

TTATTGCGGA

ATAAAAAAAC

ATCCTGGGCG

TTrTTGTCAG

CCTTGTCACT

AAACC'ITAGA

GTGAAGTCAG

ATGCGGATAA

TTGAATTGAA

AAGAAGAAGA

ATTTCAAAGA

AAACAGCAGA

CCAATTGGCG

ATAAATGGTT

ATTTTAATCC

AAGATGATAC

AAGACAGTGC

TGGCTGCATG

AAATGTATCC

LAAAATGGGAT

AGGATCAAAI

AGCAGCCGCC

CAAAGGCGAC

TAGATCTGAG TGCATCA ATACGATACC AATTAT' ACCTGAAAAA

AGCAATA

AGAATATCCA

GTAAACI

GGGAGAAACC

TATGATI

CCTAGAGCAA ACCTATG CAATCGAACC

GCGCTTV

TGGAGCAAGC

TGGACAC

TATTGGCACT CGTTTA-; AGAAACGTGG AAT' AGTTTCAACA

AAAAT

GCCTAAACAA ACGTAC GGCAAAGGAA CAACAA( GAACAAGCAA AGATGG( A=ITCCTGAA TATCA AAGTGCGGCA GGTGCC' TA7TGGTATG TGGGCT TGAGTTAGCT AAAAAT CGTACGTCCA

CAAGAT

GCGTGGTGGT GAAGG T GGCAGTTTGG CATATI CAAACTTGTG GCTTAI AGCAGAATAT GGAAGC CATTAAAGAT AAAAXI TGGGAAAGTG GCATGG G ITGGAGTTA AAGTT

.CCA

LTTA

AAG

TGG

TAA

~AAT

~TCC

;GTG

CTG

ETTG

;ATA

AACA

CAAG

CAAG

AATG

TTA

GCAG

CGCA

'TATC

CATP

ATC

GGCC

ATA7 ATAATTTTTC 9) AGCTACTGGG

CGGCAAGTTT

GCCAAACCAA

TAGACGATTI

AAACGACACT

CGGCTITGA

CTAAAGACAA

CTACGAAAGA

ATCGGAATTA

CCTATACGAC

CACCCGAATA

GATATCTAGA

CGCTAGTCAA

AGCATGACGG

ATTCGTGGAA

*TGCGGGCCTT

*GAGCGATCAT

ACTGGAATGA

AAGAA.ACCAA

ATTGTGGTA

TCAGTGATGC

ACCTAAAGTG

SCGCTACACGG

rGATTIAGGT

,GGTTTTGCG

LAACAAATTA

:CGCATGGAC

kACGATTCAT rGAAAACACT

:AAAATTCAA

rGACATTCAA

CACAAAATAT

TACAGCAACC

AGAAAGCTAT

TACcAAAAAT

TAAAACGTTT

ATCGATTGAA

GATTGTTCCG

AGCGGATGTA

GTTTIGATTAT

TGATGCTGTC

ACGAAATTCT

ACATAAGGAA

TACcAACAGA

TCACTGGCAA

GATGATGATG

'rTTGACAAAG EF111-4 (SEQ ID NO:432) DEFA NVLDVSASPT ERTNGVYDTN YFNNFSDLGA

WHGYYL~PEKS

SYYPGRLEQT

GTE-TLDIGTR

EP IELKPKQT

QKKTAEFPEY

TADFNPELAK

PPLAAWAVWH

DDiXDQI IKDK

NKELLGGFAG

YE-LDDLTIHL

LTAKDNDIQV

YNTYTTESYT

QNALVKSIET

NNMRALFDYQ

IYQETKDKEF

NGHLKW~tha4 PLI IAEEYPV

ALIFVSNRTA

NFGEVRETWN

F'IKEEEAKEQ

INTNWRSAAG

IQKDDTVRPQ

LKEI4YPKLVA

L.LKQPRGKVA

NLAASLNKLT

LIQTTLENTG

YFATKDTKYT

QQAPEYTKNA

AFKHDGIVPS

DACAI IDAVF

YHNWWYTNRD

WITLHGLTKK

VKNI(XTGETY

EEPLSLGASW

IHH-ADKVSTK

ARYFKZENKQR

MSYKWFIGMW

YNQDSARGGE

HNKNG IAEYG

VWAKATLELK

DLSQSNRMDL

TGAVFDKIQE

IDNRNYTATA

WQGYLDKTFD

AWDSWKADVA

GGNWNERNSK

SMVSDAHWQK

215 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

EF117-1 (SEQ ID NO:433)

TAATTCGATIG

GCTTTAACAG

AAGGAAACGN

GAATATA=I

AAAGGTGATC

AAGAAAAAAT

AAAGGGACAT

GAGAAGGTG

GTTGTAAATC

CACAGTCTTA

CTTTGAAAGG

GTTTTATTTT

TGAAGATTGG

TAATTGAAAG

TTTAGTGA-AA

CGGTGAAAAA

NTCAGAAACA

GGAGATTGTT

GAAAAGTGGT

TGACGAAGTG

TCAGGAGAAT

AGATT1'TCAT

GAATTTGATG

GAATTACAAA

GAGAGTGACA

TCTAGTAAAT

ACAGT'ITACG

CATGATI'CAG

'TI=ITTTACT

AAGAATCTCT

ATGGTGACGT

GTCGTTCCAG

ATCAAG=TN

GAGAATATTA

CCACGAATTA

AATTITTACTT

TCAAAATCTA

TCGTTTAAAT

TTTAATAAAA

TAATGAGCAA

CGGCT'TITG

C

EF117-2 (SEQ ID NO:434) VKR FSFFLLILLA LTGCKSGEKE FDEESLQNLK ETXQSXSETE L&NGDVRLNE YISLKGEIVE SDSRSSLIKK GDRF-ILKSGS SKYQVXNEQK KKLKIGDEVT VYGEYYGFLK GTLIESEENH DSATN EF117-3 (SEQ 10 NO:435) S

S*

S.

S.

S S

S

S.

S

S

S

S

5 *55.

*5.S S. S *5

S.

TG AAGAATCTCT TCAAAATCTA AAGGAAACGN CACAGTCTTA NTCAGA.ACA GAATATATI'T CFI'TGAAAGG GGAGATTGT AAAGGTGATC G'FrTTAFTI'

GAAAAGTGGT

AAGAAAAAAT TGAAGATTGG TGACGAAGTG AAAGGGACAT TAATTGAAAG TGAGGAGAAT

GAATTACAAA

GAGAGTGACA

TCTAGTAAAT

ACAGTTTACG

CATGATTCAG

ATGGTGACGT

GTCG'TCCAG

,ATCAAGTTTN

GAGAATATI'A

CCACGA.A

TCGTTTAA-AT

TTTAATAAAA

TAATCAGCAA

CG.GCTrT= EF117-4 (SEQ ID NO:436) EESLQNLK ETXQSXSE-TE LQNGDVRLNE YISLKGEIVE SDSRSSLIKK

CDRFILKSCS

SKYQVXNEQK KKLKIGDEVT VYGEYYGFLK GTLIESEENH

DSATN

EF1.18-1 (SEQ ID NO:437)

TGAGGGGGAA

GCTTTGITAC

AATCANAACG

TTTTCACAAG

AAAGACCCAT

CTTAGAGATG

AGATAA

AAAGTGTGTT

TGACAGCTTG,

CACAAGAATC

AAGATGGTGC

cGCTTTCTGG

AAATTAAMAG

AA.AAAGAA.AA

TGGCAAAAGT

TGGCCTTTGN

ACAAACGAAT

GGAAGATTCA

CGATGGGAAT

GTGGGGATTG

GCGAAAGATG

GATTTCTCTA

CCTATGATTG

AGTAGATGCC

GGATGTACCG

TCGCAGGCGT TTTCTGTTCA AGTTCATTCA

AGGAATCGGC

TGTCAATTAG

TGACATCAA-A

GGATGCTCAT CACGcAAATC AAAAAAGAAA AAGCATTCA ATI'TCATTCG TTGGATCGTT EF118-2 (SEQ ID NO:438) VLKRKV GIVAGVFCSA LLLTGCGKSA KDEFIQGICN XNAQESGVXD FSMSISDM(F SQEDCAQTNP MIGMLITQIK DASLSGEDSS RCQKRKSIQL RDEIKSDGNG CTDFIGWIVR EF118-3 (SEQ ID NO:439) GAAAGATG AGTTCATTCA AGGAATCGC AATCANAACG CACAAGAATC TGGGG=EIGN GA'ITCTCTA TGTCAATTAG TGACATGAAA TTTTCACAAG AAGATGTGC ACAAACGAAT CCTATGATG GCATGCTCAT CACGCAAATC AA.AGACGCAT CGCTTCTGG GGAAGATI'CA AGTAGATCCC AAAAAAGAAA AAGCATTCAA CTTAGAGATG AAAI'AAAAG CGATGGGAAT GGATGTACCG ATTTCATTGG TTGGATCGTT

AGAT

21 6 TABLE 1. Nucleotidc and Amino Acid Seqeuences of E. faecalis Genes.

EF118-4 (SEQ ID NO:440) KDEFIQGIGN XNAQESGVXD FSMSISD1M(F SQE-DGAQTNP MIGMLITQIK

DASLSGEDSS

RCQKRKSIQL RDEIKSDGNG

CTDFIGWIVR

EF11-9-1. (SEQ ID NO:441)

TAAAGAATAC

AGTGCAACGG

GGCGGAGTGT

GTATCGACAG

TTTIACGAGTG

GTAAAACTTC

PLATGAAAAGC

AAAACTG.GAG

CATGAATATG

TTGGCTAAGG

TATTAG

CGAGTAAAAT

AAAGGAGCTC

TGCTTGTAG

AAGAAACGAC

AAGCAACTAA

TAGAAGCTA

TAAAGCCCCT

TAGCGTTAGT

cTTTAcT1TTT

TGAAGAACAA

TTTCGGAAGG

GGAAATCGTG

TGGCTGTCAA

AGTGATTGAA

AAAACAGACA

TGGAAATGCG

CATGACTGAA

TTCCGTAGGA

GGATTGAA

TAAAATTTCT

CqIlTrTTTCA

AATAAGCTAC

&GCCATAAGG

ACAGTAGCAA

GAAACAACGA

TATGCGAACT

AA.ATGTATCA

AAGCTTACAA

CAA.AATGGAA

GAAATGACCT

AAA.ATTGTAT

CTITACTTAT

AAGAAAACAA

GGGAACAATC

AATTAGAAGA

TTACAAGTAT

AAAAAAATGG

CGATC-TATAA

CGCAGACACG

ATAATTCAGT

ATGCAAAAGA

TFI'ATTGTTA

GTCTAGTAA

GAAGGAATCG

ACCAGATCAT

TAATGATCGC

AATTGATGTT

AAATGATCAA

AGTGTTACTT

TAAGCA.AGAG

S S Sb S S

S.

S

S S

S

S

EF119-2 (SEQ TD NO:442) 'ZN KLPLLILLLG GVLLVSGCQS HKEENKSSKV STEETTVIET VAREQSKESF TSEATKKQTE TTKLEEPDHV KLLEAYGNAY ANFTSINDRN EKLKPLMTEK C IKK<NGI DVK TGVALVSVGK VTTIYKNDQH EYALLLDCEQ NGTQTRVL.LL AXVXNNKISE rATYNSVKQEY EF119-3 (SEQ ID NO:443) AGAAAACAA GTCTAGTAAA

GTATCGACAG

T'ITACGAGTC

GTAA.AACTTC

AATGAAAAGC

AAAACTGGAG

CATGAATATG

TTGGCTA.AGG

AAGAAACGAC

AAGCAAkCTAA

TAGAAGCTTA

TAAAGCCCCT

TAGCGTTAGT

CTTACTTTT

TGAAGAACAA

AGTGATTGAA

AAAACAGACA

TGGAAATGCG

CATGACTGAA

TTCCGTAGGA

GGATI'GTGAA

TAAAATTTCT

ACAGTAGCAA

GAAACAACGA

TATGCGAACT

AAATGTATCA

AAGGTTACAA

CAAAATGGAA

GAAATGACCT

GGGAACAATC GAAGGAATCG

AATTAGAAGA

TrACAAGTAT

AAAAAAATGG

CA EATAA

CGCAGACACG

ATAATTCAGT

TAATGATCGC

AATTGATGTT

AAATGATCAA

AGTGTTACT

TAAGCAAGAG 4 5 .45.

S

SS

EF119-4 (SEQ ID NO:44 4 ENKSSKV STEETT'JIET VAREQSKESF TSE-ATKKQTE TTXLEEPDFV KLLEAYGNAY ANFTS INDRN EKLKPLMTEK C IKXNGiDvK TGvALVSVGK VTTIYKNDQH EYALLLDCEQ

NGTQTRVLLL

AXVKNNKI SE MIYNSVKQEY EF120-1 (SEQ ID NO:44 5

TGAATAGGCG

ACTGCTTGTC

GTTGAAGAGA

AGCAGTGTCG

AGTCAGCTAA

TTAGTTGGAA

TGAAAAAGGG

GGGAACCAAA

CGAATGAAAA

AGTCTAACGA

CTGTAGCTGA

TATGGAAAAA

AATGTTAGCG

AGNAAAGAAA

AACGAGTGAA

ATCAGTGAALA

TTTAGATACT

TGGTAAAGGA

TT'TrGTCG GTAACCGC IT

ACAATTGATA

AATGCkAGAGC

ACAGCGATTA

GAGACTTTCA

TGCTACGCT

CAACGGAGGC

AGACAAACGA

CCACACCA

ATGCTGGCGA

TCATTCATCC

TTTATCATTA

ATCCTCTAAA

ACAAGCGAGC

TGGAAACAAT

TTTTACTACT

TGATGGTAGT

217 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

ACAAATACCG GAGGAATGAT TACGAAGGAT TCACCTACTG ATGAGTCGCG

ACCAATTACA

AGCTTAAGTA TTAGGTGGGG GCCTACTGGT GCTCCGCTAT TATTATATAA

AATTGGTGTT

EF120-2 (SEQ ID NO:44 6 VKKGMLAF FVVLAVLSLT ACREPKXKKV TASTEASSKV EETNEKTSET

IDKTNEQASS

SVE-SNESVXN EEPTADGNNS QLTVADLDTT AINAGDFTTL VGIWKNGKGE

SLIIHPDGST

NTGGMITKDS PTDESRP ITS LSIRWGPTGA

ALLLYKIGV

EF120-3 (SEQ ID NO:447) AAGAAA GTAACCGCTT CAACGGAGGC

ATCCTCTAAA

GTTGAAGAGA CGAATGAAAA A.ACGAGTGAA

ACAATTGATA

AGCAGTGTCG AGTCTAACGA ATCAGTGAAA

AATGAAGAGC

AGTCAGCTAA CTGTAGCTGA TITAGATACT

ACAGCCATTA

TTAGTTGGAA TATGGAAAAA TGGTAAACGA

GAGAGTTTGA

ACAA.ATACCG GAGGAATGAT TACGAAGGAT

TCACCTACTG

AGCTTAAGTA TTAGGTGCGG GCCTACTGGT

GCTGCGCTAT

AGACAALACGA

CGACA4GCTGA

ATGCTGGCCGA

TCATTCATCC

ATGAGTCGCG

TATTATATAA

ACAAGCGAGC

TGGAAACAAT

TTTTACTACT

TGATGGTAGT

ACCAATTACA

AATTGGTGTT

EF120-4 (SEQ ID NO:448) KKV TASTEASSKV EETNEKTSET

IDKTNEQASS

SVESNESVKN EEPTADGNNS QLTVADLDTT AINAGDFTTL VGIWKNGKGE

SLIIHPDGST

NTGGMITXDS PTDESRPITS LSIRWGPTGA

ALLLYRIGV

EF121-1 (SEQ ID NO:449) 0*

TGAAACACAA

CATTTTTAA

GAAACAACGA

CAAGAACCAG

ACGAGTGACA

GAAACGTCAA

ACGTCAGATG

GTTGGTITT

ACCG=TTAA

AATAAAGCGC

AAGTATGATG

AAAATTCAAC

GGTCTTCGTT

CCAGATTTAA

CCTCGTGTTA

GAGTTGAAAG

AATAGTGATC

TATATTCTCG

ACTGTACCGC

GTTGCTAAAP

ACGAATGTTC

CCAGCGTTT;

ATTAAAGGAJ

GTTCAAAAAC

AAArrrACCTC ACcTTAGTGJ GGAr-GAAATT

TGGCTGTTGC

GTCAACAAAG

TCArrrACACA

GTGTCAAGCA

TCGCTGAAAA

TTCATGGTCA

CCCAAGTAAG

TTGATAATGG

CTTTAGTGAA

CAATGGTTT

AAGAAGCCAC

TTGTTGAAGG

AAGTGGAT

CITCGCTTAA

CTAACGATC-A

CGGCTGCCAG

GTCATGACCA

TAGGGGGACC

ATGCCGATAA

CAGCAGATGA

TTCAGCAGGP

TTCCCGA-AGC

;AAGTAACGGC

;CGCGGAAGAI

'GTGTTCCCAJ

TTGAAAA-AG TI GTTGATAGCG C TTCAGAACCA G GGAAACAACA C GTCACAAGAA

AGAAGAAACG

A ITATGGAAT

TACAGTCGTT

CGACAATATT

TGAAAAGACC

GGGAAATCAT

TTTTCCAATC

GACTACCACG

TATCGGCTTA

TTTTI'TACCT

GGCTGACATT

TGCCGACCAA

CCTI'TCTTT

GAAAGATACG

.GTCGGGAAGTG

AGCAGTTAAG

GATCGGCACA

ACAATTACAA

CGCACAATTA

TTCCTATGCC

r' TALACGGTGAA TGAG-CTTTA AAAAAGTGAA GTGGGGCATG :CAAGTGTTA CTAGTACGGC

ATATGCAGTA

TAACAAGTA CCACCGATTC

AAGTAGAAA

;ACATCAAAC AAGAAGCACC

AAATCAGGCT

CCACAGCAC CAACAGAGAC

GACGAATTTA

kGCACGCCGC AAAAA6ATAAC

AAT'ITTAGGT

rGGTCTTATG AAGATCATA-A

AGAACTACCA

kACCAAGTCC GGGCACAAAA

CCCAGCAGGC

CAAGGCACTA TTTTAACAGA

TGACTTGTAT

CATCCAATGA TCACCGCCAT

GAATGTGATG

GAGTTTAATT TTGG'FTACC

GTTAATCAAA

TTGTCTGCGA ATACCTACAA

TAAGGAAGAT

AAGGAACTTC A'TIT AATCA AGATGGGCAG ACAATTCCGC ACATTCCTIT

GTGGGATGGC

TTGAA-AGALAG AAGCAGAAA6A

AGCAGTTACT

ATTGTTGCCT CGATTCATGC

GGGACAACAA

GTAATTGAAA ATCTCGCGGG CArTTCATGCG ACCAAGCAAG GAGCAGCGCC

GAATGGAA;A

GGGACAGAAG TTGTCAAAAT

TGATCTTTCA

CAAGAAGGTA CAOCAACGAT

TGTACCAACA

GCAGCGACAA AAGAATACCA

TGAAAAAACG

GCAACAGCTG ATITTACC

A.AAACAAGAA

CCAACAGCGA TCATTTC'ITT

AATTAATAAC

AGTGCGGCAG CGCTGTTAA

ATACGACAGT

ACCATTTTTG ATATCTACAA

ATACCCGAAT

AACTTACTGA AGTATTTAGA

AAAACAAG

218 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. foecalis Genes.

j. S

SS

*9 0* S S

SS

S9 9 0 9 S S 9

GCGTACTATA

GTATATAACT

GGTGAACGAA

ACGATTGCTA

GAACCTATTA

AAAAAAGGAA

AATTTITGATG

ATTCCCACTT

CGTAATGCGG

GGCCGACTAG

CAAGAAAACC

AATTT'CTCCA

GCGGTGGGAA

CTGA.ATrrrC

GATCCGTATA

CCAGAAACAG

ATTCCAGAAG

TTCGTGGTTA

ACGCTAGCAG

CATTCGCATA

GGTAATTATT

ACAACAAAAT

GCCATCGTTC

TATAFICCAI

GGGAATTTGP

GATITTTGCAC

GTTGGGGATC

GGCGCCCAAC

AATGGCACAI

CTACATGTT.

GCTAGTGAC(

GAAATTAAA2 ATGGCTACCj

CGAGAAGAA(

TTGCGGTTG'

GGCGAACGAK

ACGCCAAAAI

AATAAACAA

TATGGCGGC'

CTGTTCGTT

TAG

ACCAAACACA

ATGACATGAT

TTGTAGATGC

TGAATAATTA

AAAATTCTCA

CTCTTGATCC

AAAAATGGCG

CTCCTGATGG

GCTTTGAT

AAGCAGGGALA

CAACCTTGAT

AAGGCGCGGA

ATCACGAGTI'

CGATTTITATC

CAATCGTAGA

CAACGAAAAC

TAGAAGCAGT

CTGGGCATTT

AAACCCTTAC

CAGCCGTCGA

TAAATAATGI

TGATTTCAGC

ATGAAGCACC

TCACATTGG;

LTI'GGTGATGC

TAACTAATG(

TCATTGCTG'

TTAAAGAAAr.

TTTTACTAGj r' CAAGCTC CA

'AAGGCAATG,

k ATCGGCAA-Al k ATGATITCT'

GGATTTCAC

r ATCGTGCAG k. TTGTTTCTA

-CAGATCCAA

A ACCAAGCGG T TTTTACCTA G GACTrCT'I

GCCAGATGAT

TTCTGGAGTG

GAAAATTGAC

TCGTTACGG-C

TCCAGAAACC

AGAACAAGAA

TGCCAAAGCA

ACGTACACCA

AGATAATGCA

AGGCGAATTA

GGTGGATGCA

TATGGCCAAA

TGATTTTGGT

TAGTAATACG

AAAATCCGGG

ACACcCGAAA

GATTAAGGAA

AGGCGTAGAT

TCAAACATAT

A.AGTGGCAA-A

TGGGATCGTC

AGCAGAGCTG

TACGAATTTTI

TGGACAACGA

GATTATGTCA

TGGCGGCAT'I

LGTTACCTTTrI C GT'TGAAATC

TGATGCTGGC

r TCGTATCCAC k~ AACAGGACAJ ACAAAAGTTr T AGcTGCTGG'.

T AGATTCTGT( C AACGACGAT' T TTCGGAAGAX A ACCAGACCC( G AGCAAGACAI A AACGGGTAC, 'C TTCTGGCTG' TGACCATTA G

GACTACAAGAT

GGCCCAACCGC T GGTTI'AGCTA G T1TACGAGGAA TI

ATCGAACGAA

ATCGAATTAG AACGCCGCCG C

TATACCATTA

GGTATGGCGC C GGGGATGTTT I GCAATGAATG

TAGAGATT=

TAITACAAAG

AAAAAGTTTG

AACGTAGAGA

AT1'AAAGAGA

GAAACGACGC

CCTGAGTTAG

CGTTATGGCA

ACAGCACCAG

CTAGA.ATTGC

AACGCTGAAA

GAAAATGTGC

TATGGCCAAG

'CGCGCTr.ATA

GGCAATAGCA

TCTGTTCCII'

CAACCAAAAC

TATGATTCCA

SACGATTGTCG

r GAACCATI'GG P GGTGATGGTT1

TTAATTGAAT

r GATTTA~cAC k GCTTACAAAG 3, AAACCAACAC

ACCAATCCAT

A GAAACAGAAA G TATATTTATA

TTTAATCCA

TGACATTTCA

GGATCCTI'-C C CCAAGGGAT T

'GATTGTTFGA

,T'rGGTCAAT G.AATGACGG C TATTACGAA P .'CCACACAAA ;TCTAAAAAC C 'CCAkAGGATT .AGTTGGT1'A

ACTACCTA

MTGCAGTGG2

:CATTGTAGG

kGCGTGACATT

AGTACGCGGA

CGCATATCTG

ATATCACTCGT

AAGTGATCTA

AGAGTGAACC

CAGAAAACCC

ATGAAAAAGT

GCACACGAGA

ACGCGTTAG

TTAAACAACG

TTGCGCAAAT

CCA'ITCCACA

TTGGCGCAAA

CAAAACCAGG

GTAGTCGCGT

ATGAGAAGAA

ACGATATGCT

ACTTGAAAAG

AATATAAAGA

AGTTAATCG

CAGAAACACC

CCGTAACAGA

CGCTTGCATT

AACGACGTAA

AACATTCGT

AAACCAGTG

AAAGAATAT

'CAAGTAGGG

'ATA~rAAG

'ATTGGGACA

ACTCTTrCAA

LCAAGATGTC

G.ACGTTCAT

:'FTAAAGAC

CCAATCTCC

M'ATGCCATG

LAAAGACCAA

kCGGGTTTT

EGTGACGACC

rAAAGACCCG

TATNCAAGCT

GCGTGGTGAT

GATTGATGGA

TGCTCAAACA

AACTAAGAAA

GGCAGTTAAA

AATTGTCGAT

GACCAACTTA

CCAACCTGCT

GCCAATTAAA

TCAAGTAACC

AAAAGATGAG

TGGTGGTTTC

TACTCGCTTG

ATTAGGAATA

ACAATACCGG

AGGTGGTGAA

TGCAACCAGC

ACCATTCCCA

TGGAGGAGAG

AGTAGCAACC

GAAGAAAAAG

ATATGGTTTA

CAAAGCTAGT

.5

OSSS

S

9

S.

EF121-2 (SEQ ID NO:450) V'KKL SFKKVKWGMH FLMAVALIAP SVTSTAYAVE T1TSQQSSE-AV TSTTDS

EPVITQETTD

SDVHGQLWPNW

KAPLVNEKTH

LRFVEGTI'TK

LKANDQADI I

VPVGGPKDTG

AFIQEEIGTA

LPAGKI SYAT YNYDMI SGVD

IKQE-APNQAT

SYEDDKELPV

PMITAMNVMK

ELDFNQDGQP

VAS IHiAGQQN

TEVVKIDLSV

TADFLPKQE I

IFDIYKYPNT

YKIDISKPVG

SDSVKQSQET

GLSQVSTVVN

YDAMVLrGNHE DLKVGIIi3LT

SDPAASADQV

AKNADKWEVQ

KGIPEAQLQP

LVSVPINGEN

ERIVDAKIDG

TAPTETrNLE

QVRAQN~PAGT

FNFGLPLIIK

IPIPLWDGP

IENVAGIDAY

EGTATIVPTT

TAMISLINNV

LLKYLEKQGA

QPLDPAKEYT

TS IAEKEETS

VLIDNGDNIQ

IQQEATFPIL

RVTSLN'FLiPL

ILGHDHLSFT

NVPADEAVKA

QKEVTGAQLS

YYNQTQPDDL

iAMNNYRYGG

:SRKQ

TPQKITILGT

GTILTDDLYN

sANTYNKEDG

KEEAEKAVTE

KQGAAPNGKT

ATI(EY14EKTR

AAALFKYDSK

TISFNPNIRV

LASQGIQVGE

219 TABLE 1. Nucleotide and Amino Acid Seqeuences of Efaecalis Genes.

P IKNSDPETL

PTSPDGRTPN

ENPTLMVDAG

NFPILSSNTY

PEVEAVIXEI

SHTAVESGKR

IVDE-ARTNFN

FAVTNGGGIR

GTILLDDAGQ

IKNRQTQKFE

RLYRAATTID

KQNQACARQS

RGMIVDYIKK

AAAITKQDVR

DVFQGLP ISN

YKDGSGRVFD

KEI(YADXQAF

YGKVIYAQTG

AENEI(VIVDY

ADIKQGPIKV

PKLGANGGFL

PLDEKYKQYRM

LAQYKEPFPG

NPSVTEKKKY

KGTLOPEQEI

NAGFDLDNAY

FSKGADMAKA

PYTIVEKSGK

VVTGHLGVD:E

NYLNNVGIVT

I PFTLDGQR7-

GDVIAVLPFG

HVSSSIRIHY

ATNDFLAAGG

ERIVSISEE-A

GGFLPKTGTE

ERNWSI IGTW

TIMHTNDVHG

MNEVQYDAMA

KFAIVGVTT?

rrTP IWRGDT

APESEPTKKT

NVRTRETNLG

rISIAQIQVTG

DSTKPGTRLA

DGYDMLGGER

YJ(EL IGGGET

TETLALYGLL

FDEKWRAKAI

RLEAGKGELG

VGNHEFDFGL

ETATKTiPK<N

LAETLSQTYP

TKLISAAELL

NLIGDAIMSY

AQVKEMFEMS

S DEC NETGQT

E-EGISLDSVL

PKPDPKPDPK

FVGLSSSGWY

ELVNDGTLQ I

MARLKTFKDQ

EIALCYKDQL

VEKVTFKDP I

ELDITVIDGH

ELPENPAVKA

GQDAFSQPAID

VRSIPQKDEN

IVGSRVLG IE

IEYLKSATSL

PT? ETPVATN

IYKRRNKAS

EF121-3 (SEQ ID NO:4S1) ACAAAG TTCAGAAGCA GTA.ACAAGTA CCACCGATTC AAG I 6e S S 9*

S*

0 0

S*

0@ @0 0 *0

S.

0 S 0 @8

S

0Oe 0

CAAGAACCAG

ACCGTCACA

GAAACGTCAA

ACGTCAGATG

GTTGGTTTGT

ACCGTTTTAA

AATAAAdCGC

AAGTATGATG

AAAATTCAAC

GGTCTTCGTI'

CCAGATTTAA

CCTCGTGTTA

GAGTTGAAAG

AATAGTGATC

TATATTCTGG

ACTGTACCGG

GTTGCTAAAA

ACGAATGTTC

CGAGCGTTTA

ATTAAAGGAA

GTTCAAAAAC

AAATTACCV.

ACCTTAGTG;

GCGTACTAT;

GTATATAACI

GGTGAACGAi

ACGATTGCT)

GAACCTATT2

AAAAAAGGAX

AATTTTGAT

ATTCCGACTr

TCATTACACA

GTGTCAAGCA

TCGCTGAPAAA

TTCATGGTCA

CCCAAGTAAG

TTIGATAATGG

CTTTAGTGAA

CAATGG'TTTT

AAGAAGCCAC

TTGTTGAAGG

AAGTI'GGGAT

CTTCCCTTAA

CTAACGATCA

CGGCTGCCAG

CTCATGACCA

TAGGGGGACC

ATGccGATAA

CAGCAGATGA

TI'CAGGAGGP

ITCCCGAAGC

AAGTAACGGC

CGGGGAAGAI

GTGTTCCCA)

ACCAAACAC;

ATGACATGA

kTTGTAGATGC k. TGAATAATT; k. AAAATTCTGQ

CTCTTGATC(

3 AAAAATGGC( r CTCCTGATG'

GGAAACAACA

CTCACAAGAA

AGAAGAAACG

ATTATGGA.AT

TACAGTCGTT

CGACAATATT

TGAAAAGACC

GGGAAATCAT

TTTCCAATC

GACTACCACG

TATCGGCTTA

TTITTACCT

GGCTGACATT

TGCCGACCAA

CCTT1'CTT'I

GAAAGATACCG

*GTGGGAAGTC

*AGCAG?'A.AC

*GATCGGCAC;

ACAATTACA;

CGCACAATT.'

7TTCCTATGC( 7TA.ACGGTGAJ kGCCAGATGAK TTCTvCGAGIX

GAAAATTGA(

k. TCGTTACGG k. TCCAGAAAC,

:AGAACAAGA

3 TGCCAAAGC

ACCTACACC

GACATCAAAC

ACCACAGCAC

AGCACCCCGC

TGGTCTTATG

AACCAACTCC

CAAGGCACTA

CATCCA-ATGA

GAGTTTA.ATT

TTCTCTGCGA

AAGGAACTTG

ACAATTCCGC

TTGAAAGAAG

ATTGTI'GCCT

GTAAITGAAA

ACCAAGCAAG

GGACAGAAG

CAAGAAGGTA

GCAGCGACA

GCAACAGCT'C

CCAACAGCGP

AGTGCGGCAC

ACGAI

9

'=IC

k. AACTTACTG; r T1'GACCA~rl

GACTACAAGI

GGCCAACCG(

-GGITAGMT

-TTACGAGCA,

A

1

ATCGAACGAK

A ATCGAATTA, A AACGCCG

'AGAAAA

AAGAAGCACC

CAACAGAGAC

AAAAAATA.AC

AAGATGATAA

GGGCACAAAA

TTrAACAGA

TCACCGCCAT

TTGTTACC

ATACCTACAA

ATTTTA.ATCA

ACATI'CCTTI'

AAGCAGAAA

CGATTCATGC

ATGTCGCGGG

GACCAGCGCC

T'rCTCAAAAT

CAGCAACGAT

AAGAATACCA

ATTTTTACC

L MAT'IrC'TI

CGCTGTTTMJ

ATATCTACPJ

SAGTATTTAGI

SGTTTTAATCC

k. FIACATTT(

:TOGATCCTG(

k. GCCAAGGGAr k. TGATTGTTGJ k. AT1'CGTCAA'

TGAATGACGI

AAATCAGGCT

GACGAATITA

AATTTTAGGT

AGAACTACCA

CCCAGCAGGC

TGAC ITGTAT

GAATGTGATG

GTTAATCAAA

TAAGGAAGAT

AGATGGGCAG

GTGGGATGGC

AGCACTTACT

GGGACAACA-A

GATTGATGCG

GAATGGAAAA

TGATCTTTCA

*TGTACCAACA

*TGAAAAAACG

*AAAACAAGAA

*AA'rTAATAAC

ATACGACAGT

ATACCCCAAT

AAAACAAGGG

AAACATTCGT

AAAACCAGTG

CAAAGAATAT

r TCAAGTAGCG k. TrATA'rTAAG r TA E'GGGACA 3 CACTcTTCAA *0

OSSS

0

S

8SOO e.g.

5 *000 00 S 0* *5 EF121-4 (SEQ ID NO:452) QSSEAV TSTTDSSRKQ EPVITQETTD IKQEAPNQAT SDSVKQSQET TAPTETTNLE- TSIAEKEETS TPQKITILGT SDVHGQLWNW SYEDDKELPV GLSQVST'JVN QVRAQNPAGT VLIDNGDNIQ GTILTDDLYN 220 TABLE 1. Nucleotide and Amino Acid Seqeuences; of E. faecalis Genes.

KAPLVNEKTH

LRFVEGTTTK

LKAIWQADI I

VPVGGPKDTG

AFIQEEICTA

LPAGKISYAT

YNYDMI SGVD P IKNSDPETL PMITAMVMKl

ELOFNQDGQP

VAS IHAGQQN

TEVVKIDLSV

TADFLPKQEI

IFDIYKYPNT

YKIDISKPVG

RGMIVDY I.KI

YDAMVLGNHE

OLKVGIIGLT

SDPAASADQV

AKNADKWEVQ

KGIPEAQLQP

LVSVJPINGEN

ERIVDAKIDG

KGTLDPEQEI

FNFGLPLIKK

IPHIPL~WDGP

I ENVAG IDAY

EGTATIVPTT

TAmI SLINNV

LLKYLEKQGA

QPLDPAKEYT

ERNWS IIGTN IQQ EATFPIL

RVTSLNFLPL

ILGHDHLSFT

NVPADEAVKA

QKEVTGAQLS

YYNQTQPDDL

IAMNNYRYGG

FDEKWRAKAI

SANTYNKEDG

KEE-AEKAVTE

KQGAAPNGKT

ATKEYHEKTR

AA.ALFKYDSK

TISFNPNIRV

LASQG IQVGE ELVNDGTLQ I PTSPOGRTPN A EF122-1 (SEQ ID NO:453)

TGAAACACAA

CATTTITTAA

GAAACAACGA

CAAGAACCAG

ACGAGTGACA

GAAACGTCAA

ACGTCAGATG

GTTGOTTTGT

ACCGTTTTAA

AATAAAGCGC

AAGTATGATG

AAAATTCAAC

GGTCTTCGTT

CCAGATI'AA

CCTCGTGTTA

GAGTTGAAAG

AATAGTGATC

TATATTCTCC

ACTGTACCGG

GTTGCTAAAA

ACGAATGTTC

CGAGCGTTTA

ATTAAAGGAA

GTTCAAAAAC

AAATTACCTC

ACCTTAGTGP

GCGTACTAT;

GTATATAACI

GGTGAACGAI

ACGATTGCT)

GAACCTATTJ

AAAAAAGGAk AATT1'TGAT(

ATCCGAC?

CGTAATGCGI

GGCCGACTA,

CAAGAAAAC

AATITrCTCC

GCGGTGGGA

CTGXI'TTT

GATCCGTAT

GGAGGAAATT

TGGCTGTTGC

GTCAACAAAG

TCATTACACA

CTrGTCAAGCA

TCGCTGAAAA

TTCATGGTCA

CCCAAGTAAG

TTGATAATGG

CTI'TAGTGAA

CAATGGTTTI'

AAGAAGCCAC

TTGTTCAAGG

AAGTTGGGAT

CTTICGCTTAA

CTAACCATCA

CGGCTGCCAG

GTCATGACCA

TAGGGGGACC

ATGCCGATAA

CAGCAGATGA

TTCAGGAGGP

TTCCCGAAGC

AAGTAACGGC

CGGGGAAGAI

GTGTTCCCAI

ACCAAACACJ

ATGACATGA'

k rGTAGATG( k. TGAATAATT, k. AAAATTCTG, k. CTCTITGATC 3AAAAATGGC' r CTCCTGATG S GCTTTGAT' 3 AAGCAGGGA C CAACC7I'A A AAGGCGCGG A ATCACGAGIl 'C CGATTTTIT 'A CALATCGTAC

TGTGAAAAAG

GTTGATAGCG

TTCAGAAGCA

GGAAACAACA

GTCACAAGAA

AGAAGAAACG

ATTATGGAAT

TACAGTCGTT

CCACAATAT

TGAAAAGACC

GGGAAATCAT

TTTI'CCAATC

GACTACCACG

TATCGGCTTA

TTTTTTACCT

GGCTGACATrI

TGCCGACCAA

CCTTTCTMr

GAAAGATACC

GTGGGAAGTC

AGCAGTTAAC

GATCCCCACI

AC A 'TACAJ

CGCACAAT]L

TTCCTATGC(

pTAACGGTGAI k. GCCAGATGA! C. TTCTGGAGTI

:GAAAATTGA

k. TCG ITACGG N TCCAGAAAC

CAGAACAAGA

G TGCcAAAGC C ACGTACACC T AGATAATGC A AGGCGAATI .T GGTGGATGC A TATGGCCAJ 'T TG;ATTrI'GC IC TAGTAATA( :A AAAATCCGC

TGAGCTTTA

CCALAGTGTTA

GTAACAAGTA

GACATCAA.AC

ACCACAGCAC

AGCACGCCGC

TGGTCTTATG

AACCAAGTCC

CAAGGCACTA

CATCCAATGA

GAGTT.TAATI'

TTGTCTCGCGA

AAGGAACTTG

ACAATrCCGC

TTGAAAGAAC

ATTGTTGCCI

GTAATTGA-A

ACCAAGCAAC

GGGACAGAAC

CAAGAAGGTI

GCAGCGACAJ

kGCALACAGCT( k CCAACAGCQi k AGTGCGGCA(

:ACGATTTTTI

k AACTI'ACTG.

r TTIGACCATT

GACTACAAG

C GGCCAACCG C GGTT'AGCT C TTACGAGGA A ATCGAACGA A ATCGAATTP 'A AACGCCGCC A TATACCAT" .A GGTATGGCC :A GGGGATGTI LA GCAATGAX7 'T TTAGAGAV1 :G TATTACAAL .G AAAAAGTT AAAAAGTGAA G' CTAGTACGGC A

CCACCGATTCA.

AAGAAGCACC A CAACAGAGAC G~ AAAAAATAAC k AAGATGATAA A' GGGCACAAAA C TTI'TAACAGA T TCACCGCCAT

G

TTGGT1'TACC C ATACCTACAA TI

ATTTTAATCA

ACATTCCTT G

AAGCAGAAAAA

CCATTCATGC G ATGTCGCGGG C GAGCAGCGCC C TTGTCAAAAT kCAGCAACGAT k. AAGAATACCA

'ATI'TTACC

k TGAT'IrCTTT 3 CGCTG'ITTAA 3ATATCTACAA A~ AGTATITAGA A GTT'IAATCC A TrGACATTTC C T'GGATCCTGC A GCCAAGGGAT A TGATT1GTTGA A ATTGGTCAAT LG TGAATGACGG :G CTATTACGAA 'A TG-CACACAAA ;C GTCTAAAAAC TCCAAGGA T LG AAGTI'CG IWA IG CACTAGGCTA kG ATGGCAGTGG rG CCATTGTAGG

[GGGCATG

LATGCAGTA

!GTAGAAAA

kATCAGGCT

ACGAATTIA

.TTITAGGT

GAACTACCA

CCAGCAGGC

GACTTGTAT

AATGTGATG

TTAATCAAA

'AAGGA-AGAT

,GATGGGCAG

TGGGATGGC

~GCAGTTACT

~GGACAACAA

;ATIGATGCG

;AATGGAAAA

['GATCTTTCA

IGTACCAACA

rGAAAAAACG k.AAACAAGAA

AXTTAATAAC

ATACGACAGT

ATACCCGAAT

AAAACAAGGG

AAACATTCGT

AAAACCAGTG

CAAAGAATAT

TCAAGTAGGG

ITATATTAAG

TATTGGGACA

CACTCTTCAA

ACAAGATGTC

TCACGTTCAT

CTTTAAAGAC

ACCAATcTCC

TGATGCCATG

TAA.AGACCAA

ACGGGTT'ITTT

TGTGACGACC

221 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. [accalis Genes.

CCAGAAACAG

ATTCCAGAAG

TTCGTGGTTIA

ACGCTAGCAG

CATTCt3CATA

GGTAATTAT

ACAACAAAAT

GCCATCGII'C

TATATTCCAT

GGGAATTIGA

GATTTGCAG

GTTGGGGATG

GGCGCCCAAG

ALATGGCACA-A

CTACATGTTT

GCTAGTGACG

GAAATTAAAP

ATGGCTACCP

CGAGAAGAAC

'PTGCGGTTGI

GGCGAACGA;

ACGCCAAAA(

AATAAACAAJ

TATGrGCGGCI'

CTGTTCGTT(

CAACGAAAAC

TAGAAGCAGT

CTGGGCATTT

AAACCCTTAG.

CAGCCGTCGA

TAAATAATGT

TGATTTCAGC

ATGAAGCACG

TCACATTGGA

TTGGTGATGC

TmAACTAATGG

TCATTGCTGT

TTAAAGAAAT

T'TTTACTAGA

CAAGCTCCAI

AAGGCAATGAl

ATCGGCAAAC

LATGATI'CT'

GGATTTCACI

ATCGTGCAG(

TTTTCTW~

CAGATCCAAJ

k ACCAAGCGG( r' TITTACCTAK 3 GACTTTCTI

ACACCCGAAA

GATTAAGGAA

AGGCGTAGAT

TCAAACATAT

AAGTGCCAA.A

TGGGATCGTC

AGCAGAGCTG

TACCAATTTT

TGGACAACGA

GATTATGTCA

TGGCGGCATT

GTTACCTTTT

GTTTGAAATG

TGATGCTGGC

TCGTATCCAC

AACAGGACAJA

ACAAAAGTT

AGCTGCTGG'l

AGATTCTGTC

AACGACGAT9J V' TrCGGAAGAJ k. ACCAGACCCC 3AGCAAGACA( k AACGGGTAC) Z TTCTGGCTG(

AACGTAGAGA

ATTAAACAGA

GAAACGACGC

CCTGAG'ITAG

CGI'ATGOCA

ACAGCACCAG

CTAGAAMrC AACGClrGAAA

GAAAATGCC

TATGGCCAkAG

CG.CGCTGATA

GGCAATAGCA

TCTGTTCGII'

CAACCAAAAC

TATGATTCCA

ACGAMTTCG

*GAACCATrGC GGTGATGCGn TTAATCA-A I

SGATTTAGCAC

SCCTTACAAAC

AAACCAACAC

3 AGCAATCCA'

GAAACAGAA)

TATA=~AT

AGGTGACA-T TI

AGTACGCGGA

CGCATATCT--('G

ATATCACTGT G

AAGTGATCTA

AGAGTGAACC2

CAGAAAACCCC

ATIGAAAAAGT

GCACACGAGA

ACGCGTTI'AG

TrAAACAAGG

TTGCGCAAAT

CGATTCCACA

TIGGCbCAAA

CAAAACCAGG

*GTAGTCGCGT

ATGAGAAGAA

ACGATATGCT

ACTTGAAAAG

AATATAAAGA

AGTTAATCGG

CAGAAACACC

r' CCGTAACAGA

CGCTTGCAT

AACGACGTAA

AAAGACCCG

.'ATNCAAGCT

CGTGGTG-AT

ATTGATG-GA

.GCTCAAACA

ACTAAGAAA

;GCAGTTAAA

k.ATTGTCGAT

;ACCAACTTA

CCAACCTGCT

GCCAATTAAA

rCAACTAACC

AAAAGATGAG

TGGTGGTTTC

TACTCCCTTG

ATTAGGAATA

ACAATACCGG

AGGTGGTGA

TGCAACCAGC

ACCATTCCCA

TGGAGGAGAG

AGTAGCAACC

GAAGAAAAAG

ATATGGTTTA

CAAAGCTAGT

TAG

ZF122-2 (SEQ ID NO:454) VKKL SFKKVKWGMH FLMAVALIAP SVTSTAYAVE- TTSQQSSEAV TSTTDSSRKQ EPVITQETTD IKQEAPNQAT SDSVKQSQET TAPTTTNLFE TS IAEKEETS TPQKITILGT SDVHGQLWNW SYEDDKELPV GLSQVSTVVN QVRAQNPAGT VLIDNGD)NIQ GTILTDDLYN KAPLVNEKTH PMITAMNVMK YDAMVLGNHE FNFrGLPLIKK IQQEATFPIL SANTYNKEDG LRFVEGTTTK ELDFNQDGQP DLKVGIIGLT IPHIPLWDGP RVTSLNFLPL KEEAEKAVTE LKANDQADII VASIHAGQQN SDPAASADQV IENVAGIDAY ILGHDHLSFT KQGAAPNGKT VPVGGPKDTG TEVVKIDLSV AXNADKWEVQ EGTATIVPTT NVPADE-AVXA AT1(EYHE(TR AFIQEEIGTA TADFLPKQEI KGIPE-AQLQP TAMISLIN~NV QKEVTGAQLS AAALFKYDSK LPAGKISYAT IFDIYKYPNT LVSVPINGEN LLKYLEKQGA YYNQTQPDDtJ TISFNPNIRV YNYDMISGVD YKIDISKPVG ERIVDAXIDG QPLDPAKEYT IAANNYRYGG

LASQGIQVGE

?IKNSDPETL RGMIVDYIKX KGTLDPEQEI ERNWSIIGTN FDEKWRAKAI ELVNDGTLQ I PTSPDGRTPN AAAITKQDVR NAGFDLDNAY TIMHTNDVH-G RLEAGXGELG MARLKTFKDQ ENPTLMVDAG DVFQGLPISN FSKGADMAKA MNE:VGYDAMA VGNHEFD)FGL

EIAL~GYKD)QL

NFP ILSSNTY YKDG-SGRVFD PYTIVEKSGI( KFAIVGVTTP ETATKTHiPKN VEICVTFXDP I PEVEAVIKEI KEKYADXQAF VVTGHLGVDE TTPI{IWRGE)T LAETLSQTYP

ELDITVIDGN.

SHTAVESGKR YGKVIYAQTG NYLNNVGIVT APESEPTKKT TKLISAAELL ELPENPAVKA IVDEARTNFN AENEKVIVOY IPFTLDGQRE NVRTRETNLG NLIGDAIMSY CQDAFSQPAD FAVTNGGGIR ADIKQGIKV GDVIAVLPFG NSIAQIQVTG AQVKEM4FEM4S VRSIPQKDEN GTILLDDAGQ PKLGANGCFL HVSSSIRIHY DSTKPGTRLA SDEGNETGQT IVGSRVLGIE IKNRQTQKFE PLDEKQYRM ATNDFLA.AGG DGYDMLGGE-R EEGISLDSVL

IEYLKSATSL

RLYRAATTID LAQYKEPFPG ERIVSISEEA YKELIGGGET PKPDPKPDPK

PTPETPVATN

"Y GGFPT(T 1'TTLALYGLL FVGLSSSGWY IYKRRNKAS Lmro EF122-3 (SEQ ID) NO:455) 222 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

TG AAAAAiTGGCG TGCCAALAGCA ATCGAATTAG TGAATGACGG CACTCTTCAA

ATTCCGACTT

CGTAATCCGG

GGCCGACTAG

CAAGAAAACC

AA'TTTCTCCA

GCGGTGGGAA

CTGAAT=TC

GATCCGTATA

CCAGA.AACAG

A 1TCCAGAAG

TTCGTGGTTA

ACGCTAGCAG

CATTCGCATA

GGTAATTAT

ACAACAAAAT

GCCATCGTTG

TATATTCCAT

GGGAATTTGA

GATTTTGCAG

GTTGGGGATG

GGCGCCCAAG

A.ATGGCACAA

CTACATGTT

GCTAGTGACG

GAAATTAAAA

ATGGCTACCA

CGAGAAGAAC

TTGCGGTTGT

GGCGAACGAA

ACGCCAAAAC

AATAAACA-U,

CTCCTGATGG

P~

CCTTTGATTT AAGCAGGGAA CAACCTTGAT C

AAGGCGCGA

ATCACGAGTT

CGA'InTTATC

CAATCGTAGA

CAACGAAAAC

TAGAAGCAGT

CTGGGCAT

AAACCCTTAG

CAGCCGTCGA

TAAATAATGT

TGATTTCAGC

ATGAAGCACG

TCACATTGGA

TTGGTGATGC

TALACTAATGG

TCATTGCTGT

TTAAAGAAAT

TT'rTACTAGA

CAAGCTCCAT

AAGGCAATCA

ATCGGCAAAC

ATGATrITCTT

GGAITTCACT

ATCGTGCAC

TTGTTTCTAT

CAGATCCAAA

ACCAAGCGG

~CGTACACCAA

~GATAATGCAT

~GGCGAATTA G

GTGGATGCA

rATGGCCAAA G rGATTTTGGT TI rAGTAATACG

AAAATCCGGG

PCACCCGAAA

GATTAAGGAA AGGCGTAGAT C

TCAAACATAT

AAGTGGCAAA

TGGGATCGTC

ACCAGAGCTG

TACGAATTTT

TGGACAACGA

GATTATGTCA

TGGCGGCATT

GTTACCT'fl

GTFTTA.AATG

TGATGCTGGC

TCGTATCCAC

AACAGGACAA

ACAAAAGTTT

AGCTGCTGGT

AGA'ITCTGTC

AACGACGATT

TTCGGAAGAA

ACCAGACCCG

AGCAACACAC

ACGCCGCCG

ATACCATTA

.GTATCCGCGC

GGGATGTT

CAATGAATG

'TAGAGA ETG .'ATIrACAAAG

LAAAAGTTTG

ACGTAGAGA

~TTAAAGAGA

.AAACGACGC

:CTGAGTTAG

:GTTATGCcA

%CAGCACCAG

CTAGAA7MTC

AACGCTGAAA

GAAAATGTGC

TATGCCCAAG

CGCGCTGATP

GGCAATAGC.z

TCTGTTCGM'

CAACCAAAAC

TATGATTCCI

ACGATTGTCC

CAACCA ITG( GGTGATGGI L

TTAA'ITGAA'

GATTTAGCA(

GCTTACAMC

AAACCAACA

AGCAATCCA

CTATTACGAA ACAAGATGTC TGCACACAAA TCACGTI'CAT GTCTAAAAAC CTTTAAAGAC .TCCAAGGATT ACCAATCTCC AAGTTGGTTA TGATGCCATG CACTACGTTA TAAAGACCA.A ATGGCAGTGG ACGGTTTT CCATTGTAGG TGTGACGACC AGGTGACATT TAAAGACCCG AGTACGCGGA TATNCAAGCT CGCATATCTG GCGTGGTGAT ATATCIACTGT GATI'GATCGA AAGTGAITCTA TGCTCAAACA AGAGTGAACC AACTAAGAAA CAGAAAACCC GGCAGTTAAA *ATGAAAAAGT AATTGTcGAT GCACACGAGA GACCAACTTA ACGCGTTTAG CCAACCTGCT TTAAACAAGG GCCAATTAAA TTGCGCAAAT TCAAGTAACC CGATTCCACA AAAAGATGAG 'FTGGCGCAAA TGGTGGTTTC SCAAAACCAGG TACTCGCTI'G 3GTAGTCGCGT ATTAGGA-ATA ATGAGAAGAA ACAATACCGG 1ACGATATGCT AGGTGGTGAA r ACTTGAAA.AG TGCAACCAGC CAATATAAAGA ACCAFTCCCA G AGTTAATCGG TGGAGGAGAG CCAGAAACACC AGTAGCALACC TP CrGTAACAGA GAAGAAAAAG

S.

S

S

S

TATGGCGGCT TT EF122-4 (SEQ ID NO:456) EKWRAXAI ELVNDGTLQ I

SS

PTSPOCRTPN

ENPTLMVDAG

NFPILSSNTY

PEVEAVIKEI

SHTAVESGKR

IVDE.ARTNFN

FAVTNGGI R

GTILLDDAGQ

IKNRQTQKFE

RLYRAATI D

AA.AITKQDVR

DVFQGLPISN

YKDGSGRVFD

KEKYADXQAF

YGKVIYAQTG

AENEKVIVDY

ADIKQGPIKV

PKLGANGGFL

PEJDEKRQYRM

L.AQYKEPFPG

NAGFDLDNAY

FSKGADMAKA

PYTIVEKSGK

\TVTGHLGVDE

NYLNNVG IVT

IPFTLDGQRE

GDVIAVLPFG

HVSSSIRIHY

ATNDFLAAGG

ERIVSISEE-A

TIMHTNDVHG

MNEVGYDAMA

KFAIVGVTTP

TTPHIWRGDT

APESEPTKKT

NVRTRETNLG

NS IAQIQVTG

DSTKPGTRLA

DGYDMLGGER

YKELIGGGET

RLEAGKGELG

VGNHEFDFGL

ETATKTHPKN

LAETLSQTYP

TKLISAAELL

NMIGDAIMSY

AQVKEMFEMS

SDEGNETGQT

EECISLDSVL

PKPDPKPDPK

MARLKTFKDQ

EIALGYKDQL

VEKVTFKDPI

ELDITVIDGH

ELPE-NPAVKA

GQDAFSQPAD

VRS IPQKDEN

IVGSRVLGIE

IEYLKSATSL

PTPETPVATN

KQNQAGARQS NPSVTEKIGCY GGF EF123-1 (SEQ ID NO:457) TAAAATAAAA AATTGGTACG AAGTGAACGT TCTCITCTAT GTGTCGTTAG TAGAGGAAGG ATCAAAGAAA TGAGAAAGAA TGGTCCAATG GTAAACCGTT GGCTCTACGG GTI'GATGTGT 223 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. fuecalis Genes.

a a .t

TTGTTACTTG

GATGGCCACT

CTTCAAGGAA

TCAATCAAAG

CAGAAGAAAC

ACGTTGAACC

TTGACGTTAA

CCATCACCGA

TCCGAAGAAA

ACTGTCAAAC

ACAACCATTA

ACACCGCCGT

GATGTGCGAG

AAACCTAATA

TACACAATTA

AGTGACATTC

CCAGGAGATT

ATTCTCCCAG

CCTAGTGCGA

ACTGTGACGC

GTGATGAATC

ACCGTTGATA

GAGTACCAAA

AAAAATCACC.

GTTACCGCCA

CAAGAATTCP.

GCACTCATTA

TATTCAGTGAP

AAAGATTAC;

GTGACTGGCC

GTTCCCGTG]

GCCAGTCAA(

GGTTCGACC'

ACGTACGAA(

ACCACTGGT'

GATGGTGAA,

TTCCACATA.

TTGGACCAT

CATTCAGAAl

AGCCGTGTT

AATAATCGT

GCTGGGAGC

AA.ACCAACG

ACTTGGCGT

GTI'GATGAA

CAACGTTC'I

AAAA.AAGGI

AATGGCGCC

GTCCTACAI

ACGCCAGAI

GATAATGAJ

TATATGGAI

AACCAAGTC

TTCTAAATTA TG TAACGTTAGC AG AAGCACAACC AG CTGCACATTG

GC

ACATTCAAAT TG CTACAGCTAC AC AGACTGGTAC TC ATGAAGGTTC Ad CTGTCGCCAG Cl CGCGCGTAGC

AC

TCCATAATTT

T

ATAAAGAAGA

T(

AACAAATGAA A( AACCAr.CTTC

A(

CTGAACATCG

T.

ACGGGGACTT

T'

GGGTGATTGA

T.

ATACCGAACA

A

TTACTTGGAC

G

AAACATGGCCA

TTCATGGAACA

AAAATGGCAA

*CGACGATGA-C

*CCACGTTAACA

CATATGGCAAA

CTTGGGAAAT

T

CAGACACAAT

C

CATTTGATGA

C

AAGTGGTAAT

C

CAGTCAAGAT

kATAATCGTGT

AAAATATTAT

r' TACCTGAA

:CAGTTCCTGG

3 CTCAGTTGAC k. CAGGCTI'TAA PL CTI'ATACTAC r ATCGAAATAC 3 ATAGTAAACC T ACA.ATCCCCT T TACTCGACGC T TGAAAGTCTA C AACCCTTCAC G T'rGATTTTCC A AAG'ITATCGA "r CACGTOATGT 'C GCGAATACCA !C AATCGGTTTT CCGAGTCA1T 'A AATCTGTTAT A CAGGACAACA kT ATCGTAC'F1T IT CAATTACTGG GCACACCA CT AACTGAAG CA TAACACAA GA CAGCGCCC A.A ;AATTGAAT CA ;AAGATGTG AC ATCCGACA GA CAGAAGAA GC .CGACAAAA C 3GACCAACA G; ~AAGATCCG _A9 :;TGACCATT Cl GCAGGCGAT TI GGTGATTTA

G~

ACGTTCGT T' TCTTTAGAT A( ATICCTACA C~ CAAATTGAT A GTAGATATC A.

ACAGGGAAT A ATrAAAGAA G

GTGACGATTA

'GAGGCGGTTA

,AGTGATAATA

.ATGTTACACA

~AACTACAAC T ;CCGGATAAT T :AAACGAAAT G

AACGGAGACC

L'CATTATAAA ;GAT'FIGGC

;Z

rAAAAACACTC rcAAAATAAT

CTTAACTATG

GTTAGGCAAG

GGTAAG=TT

CTTTTTCCAT

CGCTGCCATT

GITI'AAACCT

CACCAAAGAA

cITTrCACG

TGAAGGCAAT

GGATATCACA

TAATGATAGT

AGG ETCGGCT

GACAGGAAAA

CAAACATGAT

AGACGATGTG

GGTGA'I TAC

TTTAGAACAA

AAAAATTGTC

AGTGACTTCT

AAATGGTTCA

CAGTGGCGGG

CATGGCTT TG( AACCAGCC AG( GGTTGTAG TG( .TAATACGC GC.

.GCAAGCGT TA' C PTTTC~T1 AT LATCAACGG CA ATCTACAA AC :GATAGAAA GT ~TATCAGTG AT ~TTATTTAA AT k=TTGAACT T ~CTTCGAGT TT IWGATGCAG AP rTACCTTTA A) CTCATTI'GA A9] AAGAAGATT IX A.ACAAGGCC AX.

ATCAAGCGA

T

CCTTTAAGT C( TGC'GTCGCG

A.

AAGGTGACA C ,TTCCAGATG

G'

ATCCAA-ATG 0 AGCCCAATA T

'ATGGTGAACA

'TAACG TI'C A- ;AAGTCGTTG

G

;GTI'CCTTTG C CCAAAGTTCG A ~CTIGGTCAGC A

;GTGCAGTTGA

LATITGATGG

3TACCCAATT C 3ATTTCATGG I)

ATAGGGGCGT

GTTACCGAGT

GATTGGACCG

TTACCTGCTIT

ATCACTTCCA

CATCCAATT'

ACAAAGCCAG

ATGGAAGAAC

CGTACGTATG

AGTI'ATGACA

GTTrCTATTC

CCAGATCATG

GTTATTACTG

GGTACCAACG

CGAAAAGATT

GTTAAAATCCG

TCAGCGGCGG

GAAGTCCTTC

CATGCC:ACAG

CGGAAGA

CAAGAAAT

CATTATAG

AAGATTCA

GCAGATAC

GCACAACA

ATCACGAG

TCCTCTGT

AAAACAAC

TATTTTAC

'CCTGATGG

'AACTGGTC

CAATTACC

GCAATGT

rGAGCGAA'I

~GATTCAGP

CCGCCTGI

~TTTGATCC

3AAAGATCI

:CTGAAAG

.CTTAGTC(

CAACAAAG(

CGGCGGCG

~TIAGATG'

AGATTACO.

AACCATI'C

ACCAGATT

AGCAGAAC

'AATTGACT

~TGGAA=~

~T1CAGAAC

LTTATCAAA

U".ATGCCG

GCTCGTTG

~AGAAATAJ

kTGCCAAAI

IAACGCTI

bLTGAAGCAC

TTGATTI'AJ

CGATTGCGC

1'AACCAAT(

ATGGTTCG

CAAGCGAG

TGATTGAA:

ATACCGCA

AACATGGT

TGTACIG

ATACACCC

TAACAGAA

ACACACTG

CCCATATI

GGAGTAC)

ATGGGGAI

GGACTAAl GGT1'AACAGC

GACCTTAGCG

TGCCAATGTC

AGTGGATGAC

GTTAGTCTTA

GCAACGAGCG

TTCGCCAGCC

TC'CTCGTTCG

TCAATCGACG

AGGTGATGAA

AACACCAGCA

GATTCCAGAA

TGGCAATTIG

*CTATGGAACC

*CACGTCTGAA

TGGGCGGGGC

AGTGATTCCA

AACGCCCAAT

AACAAATCCA

r' CTATGAGTTA

:AGATGAATAT

CGTATCGTCTT

A. TGTGCCT

CTGAAGCAACT

A. CCAAGCCAAT C AAAAGACCAA C =FACATTTA T TCTCGAAGGA T TTTACATGAT T CGAAGGCGAT A TCATGCCACA

ATTCAACGATT

T GATTACGGAT T CAAACATACA LC TCGTAATGCA ~C AAGTGATGCC

~ACAATCCTGCG

;G AAACAATCAT

.TGCGCAAAAA

'T TAATTI'AAGT CA AAccTATTTG 3T TlGAAAAAGTG A~A AAACCAAAAT

ITTAACACCTCT

TC TTATACAAAC CC CCAATCAGTG CA TGTAATGATC TC ACCA.AACCAA GA CGGAACTATT GA AGTTACCACC 'A AGCACCTTAT LGA CACGGTATCC CA CAATCGCGAT ~cGAAAATTCAC GTGCTCGTTG

ACATTGATCA

224 TABLE 1. Nucleotide and Amino Acid Seqeuenccs of E. faecalis Genes.

CTGA.AGAAAA

GACCAAGCTA

TTTGGTGGGT

ACAG'TTCGG

GAAGGAGCAC

GATGAGAAGG

CCGTTTACTC

rTAGAGGTGG

GGCTATCT'T

ATI'CGTGATG.

GACCAAGCAG

GCAG'ITCGAG

CCAGGAA.AAT

CCAAGTGCTI'

GCTAACTTTG

F1'ATTAAGTG

TTGACGACAA

TTTGTAGAAA

ATTGCTGAGA

TACAAAGGCG

GT=TGAAT

GATGCCAAAA

GCACCAAAAT

AAAGGACGCG

TTTAAATTGT

AAAGATGG

GATGCAACGG

TCAAATGATA

GGACGTAAAG

GCCGATGAGC

AAAGTTTCTG

GGGCATTACG

CATCCAT'ITG

ATCAATTATC

GGTGCGGTGI

ATGTCTGACP

GTGGAGACAC

ATTGCCAAAC

TACCAAGGTC

GAA'ITAAAC

AACCAAGGC J

AAAGCACCGC

GCCAAAGACI

TCCAAAAAAJ

CTTGG'ITTA(

TTGGTCGG7I

CAGCCATGGA

AAACACAAGT

TGCCACAAGG

ACGANPI'AGC

AACCAACCAT

GTAAAAAGTT

ATTGGGAAGA

ATAGTTTAAA

TAGACACGAC

TTCATCTCA-A

GCAATCCATT

AACACTTAGT

ATCAATTTGT

TCACGATTGC

TTAACTATCA

GTGCCACAT

ATAATCAAGG

CCAAAGCGCC

AAAATGCTGG

CTTTCCAAAT

TATATGATCA

TTATCTTAG-

TACCAGATGG

ATTTCAAAGC

CCGTCTTTAP

ATCGAATCG;

CATI'GGCTA]

aTGGCTATA9 AACAACCAC9

TCAACGAGC.'

AGAATCAGC(

AAATAACAA(

TTITTAGTGGJ

ATGTAACCG(

AAGGAACTG(

TTAAGGTCA!

AGCAAGGCK

AAGCGCCAA,

ACAACCAAG,

CTGCCAAGC'

TGACAGATG

TAGTTCAAG

ATGGTI'ACC

k AACCTGAAC k. CAAAACCAA

:CGAAAACCA

r TGGCAAGTI

TGAGACGACT

CCTACGTGAA

GCAATACA'TT

TAAAGGCCGA

TATTAAAAAC

AGTCAAkTGCT

AGTTCCCCTT

ACCAGGGCTT

CCCCAAACOA

AATGCTTAAT

AGCAGGTGCT

TI'CGGATGCA

GGAAACCAAA

AGCAAGCCAT

AGGCACGGCT

TAAAGTGC'T

GGAAATTGTT

AACAGGCTAT

TAAACCAGCG

CGTGAAAACG

*CAATAA.ACAA

AGACTTGGCG

CGCAGATTAT

TGATCCGGAG

GAAAATTGAT

AAACGGGGAA

GGAGGATTA

CGTCA.ATAAA

7ACATGAGTTA kAGGTC-AAACC

'CCAAGGTTC-A

GATAAGAC'I

AACGAAAGC;

:CCAATTAGG;

ZTCAATTAACC

r TGATGAAAC; k AGTCATTGCC TAGCTATCT9 3CAAACCAGC( T GGTGAAAAT' C AGAGACAGG( T GAACCACTI'i A ACTGTCTAA( T CGTGAATGC( A TCAGCCAAC, A CACACA-AGT, C GCTCTTCTA

ATTTTAGCAG

GGTACAGTAG

TTGGTGGAGA

GTCATTACTA

GATGTCA-ATA

CGCTTTAAAT

GCCCCATC

TATCAGTTCA

TCATCGTGA

TACCAAGGT

GAATT ETCAG

AAC-GGAAAAG

GCGCCAGCAG

CGGGGCAAAC

AAATTAATCA

GATGCGAAGG

GCAGAGCACT

TTATTAAATA

GTCGTGGTI'G

AATAGCGCAC

TCATAGGGA

CCAGGTACCI

ATTA'PTTATC

ATTTTCCAAM

GCCA.ATGCGP

AAAATCTTTC

GGTGCTGGT;

CAACCCATT1

GAGTTTGTA;

TTAGCGGGTC

CCGATAACA'

GGCGAAA~r'

LCCAACAGGC'

AAAGAGCAG(

AAAGAAAAC(

GGGCA.AACCI

AAAAACT1'AI r' CTTAAT-GAA

:ACTGTGGTA

r~ GATCAGCAA

SCAAACTGTC

k. CAACCAGGA

CAAGCTGTC

3 GGCACGTI= A, ACGAAACAA C AATTAC ITC T AAAAAGAGC

GCGCCCA=I

ATGCCACCGG

CAAAAGCACC

TT'-ATGAAGA

AAGTATrTTT

TAGAGC-ATGC

GAACCAACGC

CAGAAATCGA

CACAAAATAC

CTGCTGAACT

TCCTTGACAC

TCACAGTGAC

GGTACCTTT

CAGCAAgCAGT

AAAAAGATGT

GAGAAACGAT

TAGCCCCAGG

CCACGCCAGT

CTAGTGACAA

ACCAACCATT

TTACAGCAAC

ATTATTACAA

CTGAATTAGT

TAGGGGCCT

ACCCACTTCC

AAAGAGAAGT

LGCTATCAATT

ATTTAGT

k ATATCAAGC CAGTTTrGC r TCTTGA.ATCG r ACGCTAAAGG- I' ATCTGTTAGP CAATrC= 3 AAACAGGTG; 3TAGATGGAC;

CACCGGGAAC

A, CGCCAAGCGC C TTAAAGCAC( A AGA-ATGCC' G CTCGTTCM A AATATACCT G CATTCACTA' G ITAACGAGAX .G CAGCTAGAG, T TTGTCTITTAN !A AGAAATAA

CCAAATTTGG

GCTTATCACA

AGAAGGCTAT

AACTTCACC

ACAAAAAATG

CGTAACCACG

GAATGGC CAG

AGCACCGACA

GAGCGGACAA

AATTAAAAA

CACAGGACAA

GGA PTTAGCC

AAACACTGAA

TA'CAGCAACG

GAATGGACAC

TCAA.ACAGGC

AAAATATCGC

CCCATITGAA

CTTTGTGAGT

AGCAGGTG7CT

GAGTGGCAAA

AGAAATCAAA

AAAAGTAGAA

CGCCAATTTC

AGGAACGATT

AACTGCTGAA

AGATGAACTCG

GAAGAAGAAT

AGAAGTAATG

AATTTACAAT

TGCAGGAGAA

GCTAA.ATGAA

CACAACGCTA

AGGCGATCTT

LAGCATTGGCA

AACCAATCTG

GTATCCTI=

AAGCTTTACG

TTTTAT'rAAT rAGCAGOTOCT r' ACGTTCTGAC r' TGTGGAAACA r TcGcccAACA k. ACAACcTGTA k. GACAGGTTGG r CGGCCTCATG EF123-2 (SEQ ID NO:458) MRKNGPMV NRWLYGLMCL LLVLNYGTPL MALAEEVNSD GQLTLGEVKQ TSQQEMTLAL QGXAQPVTQE VVVHYSANVS KKQIQIELNQ QALADTLVLT LNPTATEDVT FSYGQQQRAL SANEGSTEEA STNSSVPRSS EEIVASITKA IESKTTES-, IKAAHWAAPN NTRKIQVDDQ TLXTGTDPTE STAITS SPAA VKPRVAGPTD I SDYFTGDET TABLE 1. Nucleotidc and Amino Acid Seqeuecoces of E. faecalis Genes.

TI IDNFEDPI

PNKPGSGDLV

GDWVIDIPTQ

VTETWPTGNT

YQTTIDEAVI

EFTWEINYNY

DYKVVINGDG

SQQNI IKNTG

TGAQLTLGKD

DHYRNTAAID

NRLVDAFLTO

WRVDFPNDSR

KGGEYHKDDF

PDKSVILEEC

QVS ITGNGSE

QAKTQVLREC

GAQPTI IKNI

ZVDSLKPGL)

QAGNPLAGAJ

SAFTIAASDI

TTNNQGEIV

KGAFQIVKfl

PKLPDGADY

KLYRIENGE

NDKQPLDEL

VS=EITTDKT

NYQGTAQLT

ETQAPTSYL

FKVTDAETC

KDKPELVN.D

YLNPDGTPAT

DAEGNVYTY

EDLPPWVIP I

FKSVKVYELV

PDGGGDVPFK

GEQTIPKDQA

SFAIDFU4DV

AVDYQNSTIG

FMVEITRNAD

WTDEAGNNHH

P1 LTNQTYLA

TYVIEFKTSV

DHVYWHVMIN

KDYTLEVTTO

VVHGDONGDV

TVDATGVITF

\TNKVFLEKMC

QFTEIEAPTG

FSVLDTTGQP

RGKPATVIATP

k. EH-LAPGKYRI >1 SADQPLAGA\ I IYPELVKVE] K IFEREVTAEI E FVNYQAEVMC G EIYAKGLNE( Y, ENETGE-ALA( L NETPSASFT ;Q TVARSLRSD ,G TFVNEKQPV PPYKEDvTIH

TISED--TVRF

VPDTEQQ

IDK

MLDGTIKEV

NHATL.TSONN

VITDTMGDNL

TGAVKI DYKT

WTLAVNQNN

GETGFKVSF

I

SEDSKPFKPL

GSLKVYEGNI

DERVIEGSAS

GAQSVLDDV'J

NETGQQKIV%

VVDIDHSGG'-

GGLPQGQYII

EKGKKLVNAI

YLLDTTPKRI

VREHLVSDAI

NFVNYQGTA2

VETKAPTGY

IFELYDHNKQ

IRGDFKGDPE

DCSLAMEDL

3RKV&EQGQT 3HYVLVETKA 3AVFKVIDEl I AKDNQGKPP' N QGLVQVNHI S KKTKPNQP1 WNFNWS IPED

TFNERITSES

QGHFDRTP.NP

GRELS PDEYT

PNGLDAEXITV

TFEPDSLHLY

KVDG IVEGOV LMENAV ITDT

GAYAKTSDAF

PAFDLNAQKS

KpD-svEK YDaTASYTNQ

ITDTPSPNQV

KMAH IEAPYY I ATCTKGKIQL

.VETKAPEGYT

k FKLEHAVrP FIVTQNTSrCQI q G1(VTVTDLAr K LIKKD)VNGHI

LNTTPVPFE]

S LGITATSGKE I FQL.GAFANFI G AGSYELDELI L AGAVFAIYNj .P TGYLLDTTLI 'G QTVDGQTNLI LT VVLKAPFI9 ,Q PGKYTFVE-r 'T KQAARETGW VREQMflAGDY Fl DIHGDFSLD)T

HI

SAITWTVDIN

Q~

VDKNGNVTIK

GI

TATYGKrLDK

R~

svTFD)DKGNE V AVNNRVDVGT

G

YEPVPGLTMV P HITYTI'FFDV

T

GVYNAVTKEI

PTQPLTUITM E G;SSRDVTGKV S

LDPESLVIYG

M.EYRSLVTSS

KKTAMDETTII

*VSDELAKGRV

FTHWEEVPLA

*RDVHVKMLNY

GKYQFVETKA

LSGATFKVLD

-AEKNAGKPAV

)GKIIFRDLAP

(GRAVFKKIDA

D ATDGYIVNKQ

DEQNQP-.QGSP

Hl PFDVTAQLGK A4 SDIKQGKVIAK -Y GAAKLVKID K APDGYQLSKQ L GLPKTNTQVN

:,FQLPGNLK

LNDSDGRGP

AMKDQTN'PT

OTNKAYRLE

N'IDYDEANQ

VGAELVEGK

QHSEDDGTA

NSLVVKDT

'ELDANNPAL

W(tTIAVNLSN

;EPSEKNQNT

IQHGGESVK

'NVTEDGTIT

AGSTDTVSN

.AGAHFQIWD

ITI DEETSAE

PORTNANGQL

2GSAELIKKD

PAGYLLNTEP

AKGETIQTGL

VVASDNFVSY

GTYYYKEI KA

NANPLPGTIF

P IYFVVKKNS

ITFLNRAGEK

EQP IAILODLI NLAPGT-YRFv

QQKNALAGAE

AVAFTIAATA

YFFVF IGLNL VGLASWLFYK

KSKK

EF123-3 (SEQ ID NO:459) GGAAGA GGTI'AACAGC GATGGCCAGT TAACGTTAGG

AGAAGTGAAG

CTTCAAGGAA AAGCACAACC

AGTAACACAA

TCAATCAAAG CTGCACATTG

GGCAGCGCCC

CAGAAGAAAC AGATTCAAAT

TGAATGAAT

ACGTrGAACC CTACAGCTAC

-AGAAGATGTG

TTGACGTTAA AGACTGGTAC

TGATCCGACA

GCATCAGCGA ATGAAGG7I'

AACAGAAGAA

TCCGAAGAAA CTGTCGCCAG

CACGACAAAA

ACTGTCAAAC CGCGCGTAGC

AGGACCAACA

ACAACGATTA TCGATAATT

TGAAGATCCG

ACACCGCCGT ATAAAGAAGA

TGTGACCATTI

GATGTGCGAG AACAAATGAA

AGCAGGCCXI

AAACCTAATA AACCAGGTTC

AGGTGATTTP

TACACAATA GTGAAGATGG TACGGT'rCG'I AGTGACATC ACGGGGACTT

TTCTTI'AGA]

CCAGGAGATT GGGTGATTGA

TATTCCTAC;'

ATTGTCCCAG ATACCr;AACA

ACAAATTGA)

CCTAGTGCGA TITACTTGGAC

GGTAGATAT(

ACTGTGACGG AAACATGGCC

AACAGGGAA'

CAA.ACCAGCC

GAGGTI'GTAG

AATAATACGC

cAGcAAGCGT

ACGTTITTCTT

GAATCAACGG

GCATCTACAA

GCGATAGAAA

GATATCACTG

ATTTATTAA

CATTGGAACT

AGCAAGAAAT

TGCA7ITATAG GCAAGATrCA

TAGCAGATAC

ATGGACAACA

CAATCACGAG

ACTCCTCTGT

GTAAAACAAC

ATTATITTTAC

ATCCTGATGG

TrAACTGGTC

GACCTTAGCG

TrCCAATGTG

AGTGGATCAC

GTTAGTCTTA

GCAACGAGCG

'rTCGCCAGCC

TCCTCGTTCG

TCAATCGACG

ACGGTGATG-

AACACCAGCA

GATTCCAGAA

TGGcAATTTG

CTATGGAACC

CACGTCTGAA

TGGGCGGGGC

AG;TGATTCCA

AAC-GCCCAAT

AACAAATCCA

CTATGAGTTA

G=rATGCAG

AAGGCAATGT

I'IACC'TI'A ATGAGCGAAT ACTCAIUITA

ATGATTCAGA

CAAGAAGATT TGCCGCCTCGT AAACAA~ccc A'TTIrGATCG AATcAAGCGA

TGAAAGATCA

ACCTLTAAGT

CCGTGAAAGT

226 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

GTGATGAATC

ACCGTTGATA

GAGTACCAAA

AAAA.ATCACG

GTTACCGCCA

CAAGAATTCA

GCAGTCATTA

TATTCACTGA

AAACATTACA

GTGACTGGCG

GTTGCCGTGA

GCCACTCAAC

GGTTGGACGT

ACGTACGAAC

ACCACTGGTG

GATGGTGAAA

TTCCACATAA

TTGGACCATT

TTGATGGAAC

A.AAATGGCAA

CG;ACGATI'GA

CGACGTTAAC

CATATGGCAA

CTTGGGAAAT

CAGACACAAT

CATTTGATGA

AAGTGGTAAT

CAGTCAAGAT

ATAATCGTGT

AAAATATTAT

TAGCTGTGAA

CAGTTCCTGG

CTCAGTTGAC

CAGGCTTTA-A

CTTATACTAC

ATCGAAATAC

AATTAAAGAA

TGTGACGATT

CGAGGCGGT

AAGTGATAAT

AATGTTAGAC

TAACTACAAC

GGGCGATAAT

CAAAGGAAAT

CAACGGAGAC

TGATTATAAA

GGATGTTGGC

TAAAAACACT

TCAAAATAAT

CTTAACTATG

GTTAGGCPLAG

GGTAAGTT

CT1T='CGMr

CGCTGCCAT'I

GTGGGTCGCG

AAAGGTGACA

ATTCCAGATG

AATCCAAATG

AAGCGCAATA

TATGGTGAAC

TTAACG=IIG

GAAGTCG'ITG

GGTTCCTITG

ACCAAAGTG

ACTGGTCAGC

66TGCAGTTG TAmTGAMG

GTACCCAATT

GATI'CATGG

ATAGGGGCGT

GTTACCGAGT

GATTGG

AACTI'AGTCC

CCAACAAAGC

GCGGCGGCGA

GGTTAGATGC

TAGATTACGA

AAACCATTCC

AACCAGATTC

GAGCAGAACT

CAATTGACTT

ATGG-AATTGT

ATTCAGAAGA

ATTATCAAAA

AAAATGCCGT

CGTTGG TTGT

TAGAAATAAC

ATGCCAAAA-C

TAGACGCTAA

AGATGAATAT

GTATCGTC'TT

I'GTGCCTTIWT

TGAAGCAACT

CGAAGCCAAT

AAAAGACCAA

TFITACATTTA

TGTGGAAGGA

TTTACATGAT

CGAAGGCGAT

TMATIGGCACA

'rrCAACGATT

GATTACGGAT

CAAAGATACA

TCGTAATGCA

AAGTGATGCC

CAATCCTGCG

EF123-4 (SEQ ID NO:460) a.

p a

EEVNSD

GQLTLGEVKQ

KKQIQIELNQ

SANEGSTEEA

TI IDNFEDPI

PNKPGSGDLV

GDWVIDIPTQ

VTETWPTGNT

YQTTIDE-AVI

EFTWEINYNY

DYKVV INGDG SQQNI IKNTG

TG.AQLTLGKD

TSQQEMTLAL

QALADTLVLT

STNSSVPRSS

YLNPOGTPAT

DAEGNVYGTY

EDLPPVVI PI

FKSVKVYELV

POGGGDVPFK

GEQTIPKDQA

SFAIDFLHDV

AVDYQNSTIG

FMVEITRNAD

QGKAQPVTQE

LNPTATEDVT

EETVASTTKA

PPYKEDVTIH

TISEDGTVRF

VPDTEQQIDK

MNLDGTIKEV

NHATL.TSDNN

VITDTMGDNL

TGAVI DYKT

WTLAVNQNNY

GETGFKVSFI

VVV}IYSANVS

FSYGQQQRAL

I ESKTT1ESTT

WNFNWSIPED

TFNERITSES

QGHFORTPNP

GRELSPDEYT

PNGLDAEATV

TFEPOSLHLY

KVDGIVEGDV

LMENAVITIDT

GAYAKTSDAF

IKAAHWAAPN

TLKTGTDPTE

VKPRVAGPTD

VREQMXAGDY

DIHGDFSLI'r

SAITWTVDIN

VDKNGNVTIK

TATYGKMUJK

SVTFDDKGNE

AVNNRVDVGT

YEPVPGLTMV

H ITYTTFFDV

NTRKIQVDDQ

STAITSSPAA

ISDYFTGDET

FeEFQLPGNLK

HLNDSDGRGP

QAMZDQTNPT

GDTNKAYRLE

RNIDYDEANQ

VVGAELVEGK

GQHSEDDGTA

PNSLVVKDTT

TELDANNPAL

DHYRNTAAID W EF124-1 (SEQ ID NO:461) a.

TAAAATAAAA

ATGAAAGAAA

TTGTTACTTG

GATGGCCAGT

CTTCAAGGAA

TCAATCA-AAG

CAGAAGAAAC

ACCTTGAACC

TI'GACGTTAA

GCATCAGCGA

TCCCAAGAAA

ACTGTCAAAC

ACAACGATTA

ACACCGCCGT

GATGTGCGAG

AATTGGTACG

TGAGAAAGAA

TITCTAAATTA

TAACGTTAGG

AAGCACAACC

CTGCACATTG

AGATTCAAAT

CTACAGCTAC

AGACTGGTAC

ATGAAGG'ITC

CTGTCGCCAG

CGCGCGTAGC

TCGATAATT

ATAAAGAAGA

AACAAATCAA

AAGTGAACGT

TG.GTCCAATG

TGCACACCA

AGAAGTGAAG

AGTAACACAA

GGCAGCGCCC

TGAATTGAAT

AGAAGATGTG

TGATCCGACA

AACAGAAGAA

CACGACAAAA

AGGACCAACA

TGAAGATCCG

TGTGACCT

AGCAGGCGAT

TCTCTTCTAT

GTAA6ACCGTT

CTCATGGCTT

CAAACCAGCC

GAGGTI'GTAG

AATAATACC

CAGCAAGCGT

ACGTTCIT

GAATCAACGG

GCATCTACAA

GCGATAGAAA

GATATCAGTG

ATITATTTAA

CAITGGAACT

TACTTCGAGT

GTGTCGTTAG

GGCTCTACGG

TrGGCGGAAGA AGCAAGAA6AT

TGCATTATAG

GCAAGATrCA

TAGCAGATAC

ATGGACAACA

CAATCACGAG

ACTCcTCTGT

GTAAAACAAC

ATTATTTrAC ATccTGATGG

TTAACTGGTC

TTCAATTACC

TAGAGGAAGG

GTrGATGTGT

GG'ITAACAGC

GACC ITAGCG

TGCCAATGTG

AGTGGATGAC

GTTAGTCTTA

GCAACGAGCG

TTCGCCAGCC

TCCTCGTTCG

TGAATCGACG

AGGTGATGAA

A6ACACCAGCA GA 1TCCAGAA TGGcAATTTrG 227 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.f[aecalis Genes.

a a.

a a a a a a a *a AAACCTAATA A TACACAATTA G' ACTCACATTC AC CCACCAGATT CC ATTGTCCC-AG A CCTAGTCA T t ACTCTGACGG A

CTCATGAATCT

ACCGTTGATAA

CACTACCAAA C AAAAATCACG C GTTACCGCCA C CAACAATTCA C GCAGTCATTA C TATTCACTGA C

AAACATTACAA

GTCACTCCCG C

CTTGCCGTGAA

GCCACTCAACA

CGTTGGACGTI

ACGTACCAAC C ACCACTCCTG C CATCGTGAAA C TTCCACATAA C

TTGGACCATT

CATTCACAAC I

AGCGGTCTTT

AATAATCGTT

CCTGGCACCT

AAACCAACC

ACTTCGCGTG

CTTGATGAAA

CAAGGTTCTT

AAAAAAGGTC

AATGCCGCCC

CTGCTAGATC

ACGCCAGATA

CATAATGA;A

TATATGGAAT

AACCAAGTGT

CTGGTCGTTG

CTGAAGAA.AA

GACCAAGCTA

TT1TGTGGGT

ACAGTTTCGG

GAAGCAGCAC

GATGAGAAG

CCCITTACTC

TTAGACGTG

GGCTATCTIP

ATTCCTGATC-

CACCAAGCAG

GCAGTTCGAG

CCAGGAAAAT

kCCACCTI'C

MGAAGATGG

CCGGGACTT

GGTGATI'CA

rACCGAACA rACTI'GGAC

;LACATGCC

TCATGGAAC

P.AATGGCAA

GACCATTGA

GACGTTAAC

ATATGGCAA

TCGCGAAAT

ACACACAAT

ATTTIGATCA

ACTGGTAAT

AGTCAACAT

.TAATCGTGT

.AAATA'ITAT

'ACCTCTGAA

:AGTTCCTCC

:TCAGTTGAC

ACGCTTTAA

T'TATACTAC

LTCGAAATAC

~TACTAAACC

kCAATGCCCT

CAGTCGACGC

rGAAACTCT.A kACCGTTCGAC rTAT1TICC kAGTTATCGP

CACGTGATG]

GCCAATACCI

AATCGGTI

CCGAGTCATJ

AATCTGTTA)

CAGGACAAC)

ATCGTACTT"

CAATTACTG(

ACATTIOATC

CACCCATCG4

AA.ACACAAG'

TGCCACAAG,

ACGAATTAGI

AACCAACCA

GTAA.AAAGT

ATI'GGGAAG

ATAGTIAA

TAGACACGA

TTCATCTCA

GCAATCCAI

AACACTTAG

ATCAATT'C

TACGCTTCGT

TTCTTTAGAT

TATI'CCTACA

ACAAkATTGAT

GCTAGATATC

A.ACAGCAAT

AAT1'AAAGAA

TCTCACGATT

CGAGCCG'P1'

AAGTCATAAT

AATCTI'ACAC

TAACTACAAC

GCCCATA.AT

CAAACGAAAT

CAACGGAGAC

TGATTATA-AA

GGATGTTGGC

TTTACCTTTA

ACTCATTTGA

CAAGAAC.ATT

AAACAAGGCC

AATCAAGCGA

ACCT=AACT

CTCCGTCCGCG

AAAGGTGACA

ATTCCAGATG

AATCCAAATG

kAGCGCALATA

TATGGTGA.AC

'rrAACGTITTC

CAAGTCOTTG

GGTTCC'ITTG

ACCAAAGTTC

ACTGGTCAGC

TAAAAACACT GCTGCAGTTG

TCAAAATAAT

CTTAACTATC

G'I"IAGGCAAG

CCTAAGTTT

cTTrTCGAT

CGCTCCCATT

GTTTAAACCT

CACCAAAGAA

CTTTICACG

TGAAGCCAAT

GGATATCACA

TAATGATAGT

ACGTTCCGCT

GACAGCAAAA

CAAAGATGAT

ACACGATGTG

GGTGA=TAC

TTTAGAAGAA

AAAATTGTC

AGTGACTI'CT

AAATGGTTCA

CAGTCCCGGC

TGAGACCACT

CCTACGTGAA

GCAATACATT

TAAAGGCCGA

TATTAAAAAC

AGTCAATCC I

ACTTCCCCTI

LACCAGCTI

CCCCAAACGP

SAATGCTTAAI

ACCAGCTGC9 7 TTCGGATCCI r' GGAAACCAA)

TATTTGATGC

GTACCCAATT

CAT'FrCATGG

ATAGG'CGT

GTTACCGAGT

GATTCGACGG

TTACC [CCTT ATCAC~rGGA

GATCCAATTT

ACAAAGCCAG

ATCCAAGAAC

CGTACGTATG

AGTTATGACA

GTrTCTA TCM

CCAGATCATG

G ITATTACTG

GGTACCAACG

GGAAAAGATIr

GTTAAAATGG

TCAGCGGCGG

CAAGTCCTTC

CATGCCACAC

ATTTTAGCAC

CCTACACTAC

TTGGTGGAGI

GTCATTACT)

CATGTCAATJ

CGCTIAAAN

GCGCCCGAT(

TATCACPTC

LTTCATCGTG.

TACCAAGGT

GAAT'I=CA

AACGGAAAA

GCGCCAGCA

ACGTGATI'TA GTTGAT,-CAG AAGGCAATGT CTATCCAACC kTGACAAT CACGTCTCAA PTGAITCAGA TGGGCGGGGC rCCCGCCTCT AGTCATTCCA A.TTTTCATCG AACCCCCAAT TGAAAGATCA AACAAATCCA CCCTGAAAGT CTATGAGTTA AACTTACTCC AGATCAATAT CCAACAAAGC GTATCGTCTT GCGCCGGCCA TGTGCCTTTT GGTTAGATCGC TGAAGCAACT TACATTACCA CGAAGCCAAT AAACCATTCC AAAAGACCAA AAccCATA'TC TTTACATI'A GAGCAGAACT TGTGGAAGGA CAATTGACTT TTTACATGAT ATCGAATTCT CCAAGCCGAT AT'TCACAACA TGATGCCACA A'PTATCAAAA TTCAACCATT AAAATGCCCT CATTACGCAT CGTTCGTTGT CAAACATACA TAGAAATAAC TCGTAATGCA ATGCCAAAAC AAGTGATGCC TAGACGCTAA CAATCCTGCG ATGAAGCAGG AAACAATCAT TTGATTTAAA TGCCGCAAAAA CGA CGCT TAATTTAACT TAACCAATCA AACCTATTTG ATCCTTCGGT TGAAAAAGTG CAAGCGACAA AAAcCAAAAT TCATTGAATT TAAGACGTCT ATACCCCATC TTATACAAAC AACATCGTCG CGAATCAGTG TCTACTCCCA TGTAATGATC ATACACCCTC ACCAAACCAA TA.ACACAAGA CGGAACTATT ACACACTCGA AGTTACCACC CCCATATTGA AGCACCTTAT CGAGTACAGA CACGGTATCC ATCGGCATGA CAATGCCAT GGACTAAACC

CAAAATTCAG

GCGCCCATTT ccAAATITCCG ATGCCACCGG GG'ITATCACA ~CAAAAGCACC

AGAACCCTAT

STTGATGAAGA AACTTCAGCC ~AAGTATrTTT AGAA.AAAATC rTAGACCATCC CGTAACCACG C AACCAACGC C-AATGCCCAG A CACAAATCCA

ACCACCOACA

A CACAAAATAC CAGCCCACAJA T CTCCTGAACT AATTAAAAAA G TCCITTGACAC CACACGACAA CTCACACTGAC CGAF'TACC GGCTAcC'TI' AAACACTCAA 228 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

C CAAGTGCTT

GCTAACTTTG

TTAI'AAGTG

TTACGACAA

TTI'GTAGA;A

ATTGCTGAGA

TACAAAGGGG

GTTTI'GAAT

GATGGCAAAA

GCACCAAAAT

ATTCGTGGTG

AAAGGACGCG

T'ITAAATT

AAAGATGGTT

GATGCAACGG

TCAAATGATA

GGACGTAAAG

GCCGATGAGC

AAAGTTTCTG

GGGCATTACG

CATCCATTTG

ATCAATTATC

GGTGCGGTGT

ATGTCTGACA

GTGGAGACAC

ATTGCCAA.AG

TACCAAGGTCG

GALATTTAAAC

AACCAAGG I

AAAGCACCGC

GCGAAAGACI

TCCAAAAAAI

CTGGT'TAC

TTGGTCGGTr.

TCACGATFGC

TTAACTATCA

GTGCGACATT

ATAATCA-AGG

CCAAAGCGCC

AAAATGCTGG

CTTTCCAAAT

TATATGATCA

TTATCTTTAG

TACCAGATC

A TrCAAAGG

CCGTCTTTAA

ATCGA.ATCGA

CATTGGCTAT

ATGGCTATAT

AACAACCACT

TCAACGAGCA

AGAATCAGCC

AAATAACAAC

TTTTAGTGGA

ATGTAACCGC

AAGGAACTGC

TTAACCTCAT

AGCAAGGCAA

AAGCGCCAAC

ACAACCAAG

CTGCCAAC

TGACAGATGC

TAGTTCAAGI

ATGGTTACC-P

AACCTGAAC I

SCAAAACCAAJ

CGAAAACCA-1 r' TGGCAAG=T

AGCAAGCGAT

AGGCACGGCT

TAAAGTGCTT

CCAAATTG-T

AACAGGCTAT

TAAACCAGCG

CGTGAAAACG

CAATAAACAA

AGACTMGCG

CGCAGATTAT

TGATCCGGAG

GAAAATTGAT

AAACGGGGAA

GGAGGATTTA

CGTCAATAAA

AGATGAGTTA

AGCTCAAACC

CCAAGGTTCA

GGATAAGACT

AACG-AAAGCA

CCAATTAGGA

TCAATTAACC

TGATGAAACA

AGTCATTGCG

TAGCTATCT'I

CAAACCAGCC

GGTGAAAAT']

AGAGACAGGC

GAACCACTT7

ACTGTCTAAC

CGTGAATGCC

TCAGCCAACI

CACACAAGT(

GCTCTTCTA'

CGGG4GCAAAC

AAATTAATCA

GATGCGAAGG

GCAGAGCACT

TTATTAAATA

GTCGTGGTTG

AATAGCGCAG3

TCAI'AGGGA

CCAGGTACCT

ATTATTTATC

ATTTTCCAAT

G6CCAATGCGA

AAAATCTTTG

GGTGCTGGTA

CAACCCATTT

GAGTTTGTAA

TTAGCGGGTG

CCGATAACAT

GGCGAAATTT

CCAACAGGCT

AAAGAGCAGC

AAAGAA.ACG

*GGGCAAACCC

AAAAACTTAC

*CTTAATGAAP~

*ACTGTGGTAC

GATCAGCAA);

cAAACTGTCC

CAACCAGGPO

CAAGCTGTCC

GGCACGTT(

k. ACGAAACAA(

-AATTACTTCI

r' AAAAAGArGCji

CAGCAACAGT

AAAAAGATGT

GAGAAACGAT

TAGCCCCAGG

CCACGCCAGT

CTAGTGACA

ACCAACCATT

TTACAGCAAC

ATTATTACAA

CTGAATTAGT

TAGGGGCCTT

ACCCACTTCC

AAAGAGAAGT

GCTAIT GATT ArTTGTAGT

ATTATCAAGC

CAGITmGC

TCTTGAATCG

ACGCTAAAGG

ATCTGTTAGA

CAATTcCTTT

AAACAGGTGA

TAGATGGACA

CACCGGGAAC

CGCCAAGCGC

TTAAAGCACC

~AGAATGCCTI

CTCGTTCATI

SAATATACCTI

CATTCACTA]

T TAACGAGAJ; 3 CAGCTAGAGI r TTGTCTT'Ar.

k. AGAAATAA

TATAGCAACG

GAATGGACAC

TCAAACAGGC

AAAATATCGC

CCCATTTGAA

CTTGTGAGT

AGCAGGTGCT

GAGTGGCAAA

AGAAATCAAA

AAAAGTAGAA

CGCCAATTTC

AGGAACGATT

AACTGCTGAA

AGATGAACTG

GAAGAAGA6AT

AGAAGTAATG

AATTflACAAT

TGCAGGAGAA

GcTAAATGAA

CACAACGCTA

AGGCGATCTT

AGCATTGGCA

AACCAATCTG

GTATCGTTTT

AkAGCTTTACG

TITTTATTAAT

AGCAGGTGcT

ACG;TTCTGAC

TGTGGAA6ACA TGCGGCA6ACA

ACAACCTGTA

k. GACAGGTTGG r CGGCCTCATG B B B

B

B

B

B

B

B

B. B B B

B.

EF124-2 (SEQ ID NO:462) MR1QNGPMV NRWLYGLMCL LLVLNYGTPL MALAEEVNSD

GQLTLGEVKQ

KKQIQIELNQ

SANEGSTEEA

TIIDNFEDPI

PNKPGSGDLV

GDWVIDIPTQ

VTETWlPTGNT

YQTTIDE.AVI

EFTWEINYNY

DYKVVINGDG

SQQNI IKNTG

TGAQLTLGKD

DHYRNTAAID

NRLVDAFLTD

WRVDFPNDSR

KGGEYHKDDP

TSQQEMTLAL

QALADTLVLT

STNSSVPRSS

YLNPDGTPAT

DAEGNVYGTY

EDLPPVVIPI

FKSVKVYELV

PDGGGDVPFK

GEQTIPKDQA

SFAIDFLHDV

AVDYQNSTIG

F14VEITRNAD

WTDEAGNNHH

PILTNQTYLA

TYVIEFKTSV

DHVYWHVMIN

QGKAQPVTQE

LNPTATE-DVT

EETVASITKA

PPYYKEDVTIH

TI SEDGTVRF

VPDTEQQIDK

MNLDGTIKEV

NI{ATLTSDNN

VITDTMGDNL

TGAVKIDYXT

WTLAVNQNNY

GETGFKVSF I

SEDSKPFKPL

GSLKVYEGNT

DEKVIEGSAS

GAQSVLDDVV

VVVHYSANVS

FSYGQQQRAL

IESKTI'ESTT

WNFN'WSI PED

TFNERITSES

QGHFDRTPNP

GRELSPDEYT

PN~GLDAE.ATV

TFEPDSLHLY

KVDGIVEGDV

LMENAVITDT

GAYAKTSDAF

PAFDLNAQKS

KPDGSVEKVK

YDNTASYTNQ

ITOTPSPNQV

IKAAHWAAPN

ThKTGTDPTE

VKPRVAGPTD

VREQfrflAGDY

DIHGDFSLDT

SAITWTVD IN

VDKNGNVTIK

TATYCKM~LDK

SVrFDDKGNE

AVNNRVI)VGT

YEPVPGLTMV

H ITYTTFFDV GVYNiAVrKE I

PTQPLTDITM

GSSRDVTGKV

LDPESLVIYG

NTRKIQVDDQ

STAITSSPAA

ISDYFTGIDET

FEFQLPGNLK

HLNDSDGRGP

QAMKDQTNPT

GDTNKAYRLE

RNIDYDEANQ

VVGAELVEGK

GQHSEDDGTA

PNSLvVKDTT

TELDANNPAL

TwJTIAVNLSN

EEPSEKNQNT

S IQHGGESVK

TNVTEDGTIT

TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

PDKSVILEEG

QVS ITGNGSE

QAKTQVLREG

GAQPTI IKND

EVDSLKPGLY

QAGNPLAGAE

SAFTIAASDR

TTNNQGEIVA

KGAFQIVKTN

PKLPDGADY I KLYR IENGEK

I'DKQPLDELE

VSE ITTDKTG

NYQGTAQLTK

ETQAPTSYLL

FKVTDAETGQ

KDKPELVNAG

KDYTLEVTTD

WVHGDDNGDV

TVDATGVITF

VNKVFLEKMD

QFTEIE-APTG-

FSVLDTTGQA

GKPATVIATA

EHLAPGX(YRF

SADQPLAGAV

IYPELVKVEI

IFEREVTAEK

FVNYQAEVMG

EIYAKGLNEG

ENETGEALAG

NETPSASFTI

TVARSLRSDN

TFVNEKQPVS

NETGQQKIVV KQMIPEAPYY VVDIDHSGGH ATGTKGKIQL GGLPQ2GQY IL VETKAPEGYT EKGKXLVNAR FKLEI14AVTTP YLLOTTPKRF IVTQNTSGQ I VREHLVSDAN GKVTIVTDLAP NFVNYQGTAK LIKKDVbJGHL VETKAPTGYL LN'ITPVPFE I FELYDHNKQS LGITATSGKD RGDFKGDPEI FQLGAFANrFX DGSLAMEDLG

AGSYELDELD

RI4VNEQGQTL AGAVFAIYNA HWVLVETKAP TGYLLD-TTLH AVFKv: DETG QTVDGQT-NLM AKDNQGKPAT VVLKAPFINTY QGLVQVNHLQ PGKYTFVETK KI<TKPNQPTT KQAARETGWL MEYRSLVTSS AAGSTDTVSN KYKTAMDETTI LAGAHFQ IWD

VSDELAK(GRV

FTHWEEVPL.A

RDVHVKMLNY

GKYQFVETKA

LSGATF'KLD

AEKNAGKPAV

PDRTNANGQL

QGSAELIKKD

PAGYLLNTEP

AKGE-TIQTGL

VVASDNFVSY

GKI IFRDLAP GTYYYKEIKA GRAVFKK IDA NANPLPGTIF ATDGY IVNKQ

DEQNQPQGSP

PFDVTAQLGK

SDKQdKVIAK

QGAAKLVKID

APDGYQLSKQ

ITFLNRAG:EK

EQPIALGDLI

NLAPGTYRF-V

QQKNALAGAE

AVAFTIAATA

GLPKTNTQVN 'YFFVFIGLML VGLJASWLFYK KSKK EF124-3 (SEQ ID NO:463) TGCCTTCCACATAACTTATACTACCTTT=GACG GATCCAATIT TAACCAATCA AACCTATTTG C C

C

C.

C C

C

C

C

GCTGGGAGCT

AAACCPACGC

ACTTGGCGTG

GTITGATGAAA

CAAGGTTCTT

AAAAAAGGTG

AATGGCCCCC

GTGCTAGATC

ACGCCAGATA

GATAATGAAA

TATATGGAAT

AACCAAGTGT

GTGGTCGTTG

CTGAAGAAAA

GACCAAGCTA

T TTGGTGGGT

ACAGTTTCGC

GAAGGAGCAC

GATGAGAAGG

CCGTTTACTC

TTAGAGGTG

GGCTATCTI'

ATTCGTGATG

GACCAAGCAG

GCAGTTCGAG

CCAGGAAAAT

CCAAGTGCT

GCTAACTI'G

TTATTAAGTG

TTGACGACAA

rGAAACTCTA TGAAGGCAAT .ACCGI'GAC GGATATCACA rTGATTTTCC TAATGATAGT ~ATTATCGA AGGTTCGGCT CACGTGATGT GACAGGAAAA GCGAATACCA CAAAGATGAT AATCGG'TIT AGACGATGTG CCGAGTCA ET GGTGATTI'AC AATCTGTTAT TTTAGAAGAA CAGGACAACA

AAAAATTGTC

ATCGTAGTI' ACTGACTTCT CAATTACTGG AAATGGTTCA ACATTGATCA

CAGTGGCGGG

CAGCCATGGA TGAGACGAcT AAACACAAGT CCTACGTGAA TGCCACAAGG GCAATACATT ACGAATTAGC TAAAGGCCGA AACCAACCAT TATrAAAAAC GTAAAAAGTT AGTCAATGCT ATTGGGAAGA AGTTCCCCTI ATAGITTTAA-A ACCAGGGCTI TAGACACGAC CCCCAAACGP TTCATGTCAA AATGCTTAAI GCAATrCCMTr AGCAGGTGC]l AACAC~rAGT 'rrCGGATGC; ATCAATITGT GGAAACCAAM TCACGATTGC AGCAAGCGAJ TTAACTATCA AGGCACGGC GTGCGACATT TAAAGTGCT.

ATAATCAAGG G

ACAAAGCCAG

ATGGAAGAAC

CGTACGTAT'G

AGTI'ATGACA

GTTTCTATTC

CCAGATCAT G

GTTATITACTG

GGTACCAACG

CAACCAGAA

TGATTGAATT

ATACCGCATC

AACATGGTGG

TGTACTGGCA

ATACACCCTC

TAACAGAAGA

AAACCAAAAT

TAAGACGTCT

TTATACAAAC

CGAATCAGTC

TGTAATGATC

ACCAAACCAA

CGGAACTATT

GGAAAAGA~I? ACACACTGGA

AGTTACCACC

C. C C C

GTTAAAATGG

TCAGCGCCGG

GAAGTCGTTrC CATGCCACAG4

ATT'TAGCAG

GGTACAGTAG

ITGGTGGAGA

GTCATTACTA

GATGTCAATA

CGCTITAAAT

GCGCCCGGATC

TATCAGTTCA

TTCATCGTGA

TACCAAGGTT

GAA~T~rCAG

AACGGAAAAG

GCGCCAGCAG

CCGGCCAAAC

AAATTAATCA

GATGCGAAGG

CCCATATTGA

~GAGTACAGA

ATGGGGATGA

GGACTAAAGG

GCGCCCATT

ATOCCACCOG

CAAAAGCACC

TGATGAAGA

AAGTATTFT

TAGAGCATGC

GAACCA.ACGC

CAGAAATCGA

CACAAAATAC

CTGCTGAACT

TCCITGACAC

TCACAGTGAC

GGTACCT

CAGCAACAGT

AAAAAGATGT

GAGAAACGAT

CACGGTATCC

CAATGGCGAT

CAAAATI'CAG

:cAAATrTGG

GGTTATCACA

AGAAGGCTAT

AACTTCAGCC

AGAAAAAATrG

CGTAACCACG

GAATGGCCAG

AGCACCGACA

GAGCGGACAA

AArrAAAAAAA

C-ACAGGACAA

GGATITAGCC

AAACAcTGAA

TATAGCAACG

GAATGGACAC

TCAAACAGGC

EF124-4 (SEQ ID NO:464) 230 TABLE 1. Nucleofide and Amino Acid Seqeuences of E. faecalis Genes.

AF HITYTFFDV TELDANNPAL

DHYRNTAAID

NRLVDAFLTD

WRVDFPNDSR

KGGEYHKDDP

PDKSVILEEG

QVS ITGNGSE

QAKTQVLREG

GAQPTIIXND

EVDSLKPGLY

QAGNPLAGAE

SAFTIA-ASDR

TTNNQG

WTDEAGNNHH

P ILTN.QTYLA

TYVIEFKTSV

DHVYWHVMIN

KDYTLEVTTD

VVHGDDNGDV

TVDATGVITF

'NKVFLEKMD

QFTEIEAPTG

FSVLDTTI'GQA

GKPA'IVIATA

SEDSKPFKPL

GSLKVYEGNT

DEKVI EGSAS

GAQSVLDDVV

NETGQQKIVV

VVDIDHSCGH

GGLPQGQYIL

EKGKKLVNAR

YLLD'TTPKRF

PAFDLNAQKS

KPDGSVEKVK

YDNTASYTNQ

ITIDTPSPNQV

IQ4AHI EAPYY ATGTKGK IQL

VETKAPEGYT

FKLEHAVTI'P

IVTQNTSGQ I GVYNAVTKE I

PTQPLTDITM

GSSRDVTGKV

LDPESLVIYG

MEYRSLVTSS

KKTAMDETTI

VSDELAKGRV

FTHWEEVPLA

RDVHVKMLNY

GKYQFVETKA

LSGATFKVLD

TWTIAVNLSN

EEPSEKNQNT

S IQHGGESVK

TNVTEDGTIT

AAGSTDTVSN

LAGAHFQ IWD

ITIDEETSAE

PDRTNANGQL

QGSAEL IKKD pAGYLLNTEP

AKGETIQTGL

VREHLVSDAN tOKV-TVTDLAP NFVNYQGTAK LIKKDVNGHL EF125-1 (SEQ ID NO:465)

S.

TAAAATAAAA

ATGAAAGAAA

TTGTTACTTG

GATGGCCAGT

CTTCAAGGAA

TCAATCAAAG

CAGAACAAAC

ACGTTGAACC

ITGACGTTAA

GCATCAGCGA

TCCGAAGAAA

ACTGTCAAAC

ACAACGATTA

ACACCGCCGT

GATGTGCGAG

AAACCTAATA

TACACAATTA

AGTGACATTC

CCAGGAGATT

ATTGTCCCAG

CCTAGTGCGA

ACTGTGACGG

GTGATCAATC

ACCGTTATA

GAGTACCAAA

AAAAATCACG

GTTACCGCCA

CAAGAATTCA

GCAGTCATTA

TATTCAGTGA

AAAGA'ITACA

GTGACTIGGCG

GTTCCGTGA

GCCAGTCAAC

GG L'IGGACGT

ACGTACGAAC

AATTCGTACG

TGAGAAAGAA

TTCTAAATTA

TAACGTTAGG

AAGCACAACC

CTGCACAITG

AOATTCAAAT

CTACAGCTAC

AGACTGGTAC

ATGAAGGTTC

CTGTCGCCAG

CGCGCGTAGC

TCGATAAIT

ATAAAGAAGA

AACAAATGAA

AACCAGGTTC

GTGAAGATGG

ACGGGGACI'

GGGTGA'ITGA

ATACCGAACA

TTACTTGGAC

AAACATGGCC

TGATGGAAC

AAAATGGCAA

CGACGATTGA

CGACGTTAAC

CATATGGCAA

CTTGGGAAAT

CAGACACAAT

CATTTGATGA

AAGTGGTAAT

CAGTCAAGAT

ATAATCGTGT

AAAATATTAT

TAGCTGTGAA

CAG-TTCCTGG

AAGTGAACGT

TGCTCCAATG

TGGCACACCA

AGAAGTGAAG

AGTAACACAA

GGCAGCGCCC

TAATTGAAT

AGAAGATG

TGATCCGACA

AACAGAAGAA

CACGACAAAA

AGGACCAACA

T'GAAGATCCG

TGTGACCATTI

AGCAGGCr-AT AGGTGATrrA

TACGGTTCGT

TTCTTTAGAT

TATTCCTACA

ACAAATGAT

GGTAGATATC

AACAGGGAAT

AATTAAAGAA

TGTGACCATT

CGAGGCGGTT

AAGTGATAAT

AATCTTiAGAC

TAACTACAAC

GGGGGATAAT

CAAAGGAAAT

CAACGGAGAC

TGATTATAAA

GGATGTGC

*TAAAAACACI

*TCAAAATAA I

CTTAACTATG

TCTCTTCTAT

GTAA.ACCGTT

CTCATGGCTT

CAAACCAGCC

GAGGTTGTAG

AATAATACGC

CAGCAAGCGT

ACG'TTETCTT

GAATCAACGG

GCATCTACAA

GCGATAGAAA

GATATCAGC

ATTTAITAA

CATTGGAACT

TACTCGAGT

GTTGATGCAG

TI'ACCTTI'A

ACTCATTGA

CAAGAAGATI

AAACAAGGCC

AATCAAGCGA

ACCTTTAAGT

GTGGGTCGCG

AAAGGTGACA

A'rI'CCAGATG

AATCCAAATG

AAG3CGCAATA

TATGGTGAAC

TTAACGTTTG

GAAGTCGTITC

GGTTCCTTTC

ACCA;AAGTrC

ACTGGTCAGC

GGTGCACTrC TAT ITGAT~c

GTACCCAAT)

GTGTCGTTAG

GGCTCTACGG

TGGCGGAAGA

AGCAAGAAAT

TGCATTATAG

GCAAGAITCA

TAGCAGATAC

ATGOGACAACA

CAATCACGAG

ACTCCTCTGT

GTAAAACA.AC

ATTATTTTAC

ATCCTGATGG

TTAACTGGTC

TTCAATTACC

AAGGCP.ATGT

ATGAGCGAAT

ATG)WI'CAGA

TGCCCCTGT

AT1'TTATCG

TGA.AAGATCA

CCGTGAAAGT

AACTTAGTCC

CCAACAAAGC

GCGCCGGCGA

GGTTAGATIGC

TAGATTACGA

AAACCATTCC

AACCAGATC

GAGCAGAAC'I

CAATTGACTI

ATGGAATTG'I

ATTCAGAAGP

A 'TATCAAAJ

AAAATGCCG!

CGTTGGTG'

TAGAGGAAGG

GTTGATGTGT

GCGTTAACAGC

GACCTTAGCG

TGCCAATGTG

AGTGGATGAC

GTTAGTCTTA

GCAACGAGCG

TTCGCCACCC

TCCTCGTTCG

TGAATCGACG

AGGTCATGAA

AACACCAGCA

GATTCCAGAA

TGGCAATTTG

CTATGGAACC

CACGTCTGAA

TGGGCGGGGC

AGCrATTCCA

AACGCCCAAT

AACAAATCCA

CTATGAGTTA

AGATGAATAT

GTATCGTCTT

~TcTCCTTTT

TGAAGCAACT

CGAAGCCAAT

AA.AAGACCAA

TTTACATTTA

TGTGGAAGGA

TI'TACATGAT

CGAAGGCGAT

LTGATGGCACA

L TCAACGA ET

GATTACCOAT

CAAAGATACA

231 TABLE 1. Nucleotide and Amino Acid Secleuences of E. faecalis Genes.

ACCACTGGTG

GATGGTGAAA

=TCACATAA

TTGGACCATT

CA ITCAGAAG

AGCCGTGTTT

AkATAATCG T

GCTGGGAGCT

AAACCAACGC

ACTTGGCGTG

GTTGATGAAA

CAAGGTTCTT

AAAAAAGGTG

AATGGCGCCC

CTGCTAGATC

ACGCCAGATA

GATAATGAAA

TATATCGAAT

AACCAAGTGT

GTGGTCGTTG

CTCAAGAAAA

GACCAAGCTA

TT'1GGTGGGT

ACAGTTTCGG

GAAGGAGCAC

GATGAGAAGG

CCG'ITTACTC

'ITAGAGGTC

GGCTATCMT

ATCGTGATC

GACCAAGCAC

GCAGTTCGAC

CCAGGAAAA]

CCAACTCT

GCTAACTIT

TTATTAAGTC

TTGACGACAJ

TTTGTAGAAJ

ATTCCTGACj

TACAAAGGG(

G~rlrrCAA!

GATGGCAAA.

GCACCAAAA!

ATTCGTGGT

AAAGGACGCI

TTTAAATTG

AAAGATGGT

GATGCAACG

TCAAATGAT

GGACGTAAA

GCCGATGAG

AAAG I=C'

GGGCATTAC

CATCCA=F

CTCAGTTGAC

CAGGCTTTAA

CTTATACTAC

ATCGAAATAC

ATAGTAAACC

ACAATrGCCGT

TAGTCGACGC

TGAAAGTCTA

AACCGTTGAC

TTGATTTTCC

AAG'ITATCGA

CACGTCATGT

GCGAATACCA

AATCGGT=T

CCGAGTCATT

APATCTGTTAT

CAGGACAACA

ATCGTAGT1"I'

CAA'ITACTGG

ACATTGATCA

CAGCCATGGA

AAACACAAGCr

TGCCACAAGG

ACGAATTAGC

AACCAACCANI

GTAAAA.AGT'l

ATTGGGAAGI

ATAGTTI'AA;

TAGACACCAC

TI'CATGTCA)

GCAATCCAT'

AACACTTAG'

ATCAATI'TG'.

7TCACGATI'G( TrAACTATC,

GTGCGACAT

k. ATAATCAAG k. CCAAAGCC k AAA.ATCTGC 3 CTTTCCAAA r TATATGATC k~ ITATCTI'A r TACCAG;ATO SA=TCAAkAG G CCGTCTIA T ATCGAATCG T CATTGGCTP G ATGGCTATP A AACAACCAC G TCAALCGAGC C AGAATCAC G AAATAACPM 'C FITTAGTCC r. ATCTAACCC

GTTAGGCAAG

GGTAAGTITI

CTTTTTCGAT

CGCTGCCATT

GT~rAAACCT

CACCAAAGA.A

CTrrTTGACG

TGAAGGCAAT

GGATATCACA

TAATGATAGT

AGGTTCGGCT

GACAGGAAAA

CAAAGATGAT

AGACGATGTG

GGTGAT1TTAC

TTTAGAAGAA

AAAAATTGTC

AGTGACTTC7

AAATGGTTCA

*CAGTGGCGGG

TGAGACGACI

CCTACGTGA-Z

GCAATACAT2

TAAAGGCCG;

*TATTAAAAAC

AGTCAATGCI7

AGI'TCCCCT'

ACCAGGGCT'

CCCCAAACGj

SAATG.CTTAAN

C' AGCAGGTGCI C TrCGGATGCC r GGAAACCAA

:AGCAAGCGN

k AGGCACGGC' r TAA.AGTGCT 3 GGAAAITGT 1. AACAGGCTA s TAAACCAGC T CGTGAAAAC A CAATAAACA C AGACTTGGC G CGCAGXIb G TGATCCCGP A GAA.kAArGP ;A AA.ACGGGG-; ,T GGAGGATTI LT CGTCAATA; :T AGATGAGTl :A AGGTCAAAC :C CCAAGGTT( XC GGATAAGA( ;A AACGAAAG( 'C CCAAT]TAG(

GATTTCATGG

ATAGGGGCGT

OTTAC CGCGT

GATTGGACGG

TI'ACCTGC T ATCACT1'GGA

GATCCAATT

ACAAAGCCAG

ATGGAAGAAC

CGTACGTATG

AGTTATGACA

G'1TTC.TATI'C

CCAGATCATC

GTTATTACTG

GGTACCAACG

GGAAAAGAIT

GTTAAAATGG

TCAGCGGCCG

CAAGTCGIPC

CATGCCACAG

A'ITTAGCAG

LCr.TACAGTAG I TCTGAGA

GTCATTACTA

GATGTCAATA

P CGCTI'TAAA'I C' GCGCCGGATC r TATCAGTTCP TTCATCGT1GP r TACCAAGGT'] r GAATTTAc PL AACGGAAAAC PL GCGCCAGCAC T CGGGGCAAAC T AAATTAATC) T GATCGAAG( T GCAGAGCAC' TTATTAAAT4 G GTCG-TGGT'( G AATAGCGCA( A TCA'ITAGGG.

CG CCAGGTACC ,T AT'rATTTAT LG ArITTTCCAA LT CCCAATGCG- ~A AAAATCM1 .A GGTGcTGCT L.A CAACCCATT C'A GAGTTGTA IC TAGCGGG'I 'A CCGATAACP -T GGcGAAATI -A cCAACAGGC -A AAAGAGCAC

TAGAAATAAC

AT'GCCAAAAC

TAGACCCTAA

ATOGAAGCAGG

TITAATAAA

CGATTGCGGT

TAACCAATCA

A 1'CG-CGGT

CAAGCGAGAA

TCATTYCAA?1'

ATACCGCATC

AACATGGTGG

TGTACTGGCA

ATACACCCTC

TAACAGA6AGA

ACACACTGGA

CCCATATTIGA

GGAGTACAGA

ATIGGGATGA

GGACTAAAGG

GCGCCCATT

ATCCCACCGG

*CAAAAGCACC

*TTGATGAAGA

AAGTAT'HLIr'

*TAGAGCATCC

*GAACCAACGC

CAGAAATCG;

CACAAAATAC

*CTGCTG-AACI

TCCTTGACAC

TCACAGTGA(

*GGTACCTT

-CAGCAACAG'.

N6 AAAAAGATG' 1; CAGAAACGAN r TAGccccAG( k CCACGCCAG' SCTAGTGACk

SACCAACCAT

TIACAGCAA

TATTACA

C CGAATTAG T TAGGGGCCT A ACCCACIT G AAACAGAAG 'A GCTATGAA7 IT A'TPIGTAG, -A A~rATCAAC )G CAGTTFFI IT TCTTGAATC T ACCCTAAAC 'T ATCTGTTAC ;C CAAIrMCT TCGTA6ATGCA

AAGTGATGCC

CA6ATCCTGCG

A.AACAATCAT

TGCGCAAAAA

TAATTTA6AGT

AACCTATTTG

TGAAAAAGTC

AAACCAAAAT

TAAGACGTCT

TTATACAAAC

CGAATCAGTG

TGTAATGATC

ACCAAACCAA

CGGAACTATT

AGTTACCACC

AGCACCTTAT

CACGGTATCC

CAATGGCGAT

CAAAATTCAG

CCAAATTTGG

GGTTATCACA

AGA.AGGCTAT

AACTTCAGCC

AGAAAAAATG

CGTAACCACG

GAATGGCCAG

AGCACCGACA

GAGCGGACAA

AATTAAAAAA

CACAGGACAA

GGATTTAGCCC

rAAACACTGAA r* TATAGCAACG r' GAATGGACAC r TCAAACAGGC 3 AAAATATCGC r CCCATTTGAA k~ CTTCTGACT T AGCAGCTGCT C GAGTGGCAAA A AGAAATCAAA T AAAAGTAGAA T cGccAATITC C AGGAACGATT T AACTGCTCAA T AGATGAACTG T GAAGAAGAAT ;C AGAAGTAA-TG ;C AATTTAcAAT :G TGCAGCAGAA ;G GCTAAATGAA ;A CACAACGCTA LrT AGGCGATCTT 232 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

ATCAATTATC

GGTGCGGTGT

ATGTCTGACA

GTGGAGACAC

AT9TGCCAAAG

TACCAAGGTG

GAATTTAAAG

A.ACCAAGGGT

AAAGCACCGG

GCGAA.AGACA

TCCAAMAAA

CTTGGI=rAC T1'GGTCGGTT

AAGGAACTC

TTAAGGTCAT

AGCAAGGCAA

AAGCGCCAAC

ACAACCAAG

CTGCCAAGCT

TGACAGATGC

TAGTTCAAGT

ATGGTTACCA

AACCTGAACT

CAAAACCAAA

CGAAAACCAA

TGGCAAG LTG

TCAATTAACC

TGATGAAACA

AGTCNITGCG

TAGCTATCTT

CAAACCAGCC

GGTGAAAATT

AGAGACAGGG

GAACCACTTA

ACTGTCTAAG

CGTGAATGCG

TCACCCAACA

CACACAAGTC

GCTCTTCTAT

AAAGAAAACG

GGGCAAACCG

AAAAACTTAG

CTTAATGAAA

ACTGTGGTAC

GATCAGCAAA

CAAACTGTCG

CAACCAGGAA

CAAGCTGTCG

GGCACGTTTG

ACGAAkACAAG

KA-TTACTTCT

AAAAAGAGCA

AA.ACAGGTGA

TAGATGGACA

CACCGGGAAC

CGCCAAGCGC

TTAAAGCACC

AGALATGCCTI'

CTCGTTCATT

AATATACCTT

CATTCACTAT

TAACGAGAA

CAGCTAGAGA

TTGTCTTTAT

AGAAATAA

AGCATTGGCA

AACCAATCTG

GTATCGT

AAGCTTITACG

TTTTATTAAT

AGCAGGTGCT

ACGTTCTGAC

TGTGGAAACA

TGCGGCAACA

ACAACCTGTA

GACAGGTTG

C--GCCTCATG

EF125-2 (SEQ ID NO:466) MRKNGPMV NRWLYGLMCL LLVLNYGTPL MALAEEVNSD

S.

S.

S S

S

S.

S

S

S

S

IS S

S

55 5 S S 5*

GQLTLGEVKQ

KKQIQIELNQ

SANEGSTEEA

TI IDNFEDPI

PNKPGSGDLV

GDWVIDIPTQ

VTETWPTGNT

YQTTIDEAVI

EFTWEINYNY

DYKVVINGDG

SQQNI IKNTG

TGAQLTLGKD

DHYRNTAAID

NRLVDAFLTD

WRVDFPNDSR

KGGEYHKDDP

PDKSVILEEG

QVS ITGNGSE

QAXTQVLJREG

GAQPT IIKND

EVDSLKPGLY

QAGNPLAGAE

SAFTIAASDR

TI'NNQGEIVA

KGAFQIVKTN

PKLPDGADY I

KLYRIENGEE

NDKQPLDELE

VSEITTDXTC

NYQGTAQLTF

ETQAPTSYLI

FKVI'DAETGC

KDKPELVNAC

TSQQEMTLAL

QALADTLVLT

STNSSVPRSS

YLNPDGTPAT

DAEGNVYGTY

EDLPPWVIP I

FKSVKVY-ELV

PDGGGDVPFK

GEQTIPKDQA

SFAIDFLHDV

AVDYQNSTIG

FMVEITRNAD

WTDEAGNNHH

P ILTNQTYLA

TYVIEFKTSV

DHVYWHVMIE

KOYTLEVTTE

VVH-GDDNGDM

TVDATGVITI

VNKVFLEKMr

QFTEIEAPTC

FSVLOTTGQI

GKPATVIATI

EHLAPGKYRI

SADQPLAGAI

IYPELVKVE]

IFEREVTAE]

FVNYQAEVM(

EIYAKGLNE'

ENETGEALA,

NETPSASFT

TVARSLRSD

TFVNEQ P V

QGKAQPVTQE

LNPTATEDVT

EETVASTTKA

PPYKEDVTIH

TI SEDGTVRF

VPDTEQQIDK

MLDGTIKEV

NHATLTSDNN

VITDTMGDNL

TGAVKIDYKT

WTLAVNQNNY

GETGFKVSF I

SEDSKPFKPL

GSLKVYEGNT

DEKVIEGSAS

GAQSVLDDVV

NETG;QQKIVV

VVDIDHSGGH

GGLPQGQYIL

EKCI(XLVNAF

YLLDTTPKRI-

SVREHLVSDAD

SNFVNYQGTId

VETK.APTGYI

I FELYDHNKQ! E RGDFKGDPE:

DGSLAHEDL(

3 RKVNEQGQT] HYvLvETKA! 3 AVFKVIDET( I AKDNQGKPA! N QGLVQVNHL, S KKTKPNQPT'

VVVHYSANVS

FSYGQQQRAL

IESKTTEST'

WNFNWSIPED

TFNERITSES

QGHFDRTPNP

GRELSPDEYT

PNGLDAEATV

TFEPDSLHLY

KVDGIVEGDV

L1MENAVITUT

GAYAI(TSDAF

PAFDLNAQKS

KPIDGSVEKVK

YDNTASYTNQ

ITDTPSPNQV

KMAHIEAPYY

ATGTKGKIQL

VETKAPEGY7I FKLEHAVTrl

IVTQNTSGQI

IGKVTVTDLAI

*LII(XDVNGHI

*LNTTPVPFE:

5LGITATSGKI

[FQLGAFANFI

AGSYELDELI

.~AGAVFAIYNA

P TCYLLDTI'L] 3QTVDGQTNLU r VVLKAPFIN Q PGKYTFVET IT KQAARETCGW

IKAAHWAAPN

TLKTGTDPTE

VKPRVAGPTD

VREQMKlAGDY

DIHGDFSLDT

SAITWTVD IN VDKNGNVT 1K

TATYGKMLDK

SVTFDDKGNE

AVNNRVDVGT

YEPVPGLTMV

HITYTTFFDV

GVYNAVTKEI

PTQPLTDITM~

GSSRDVTGKV

*LDPESLVIYG

*MEYRSLVTSS

KKTAM~DETTI

*VSDELAKGRV

FTHWEEVPLA

RDVHVKMLNY

GKYQFVETKA

LSGATFKVu:

IAEKNAGKPA%

GKIIFRDLMI

GRAVFKKIDI

D ATDGYIVNK k DEQNQPQGS] 9 PFDVTAQLGI XI SDKQGKVIA] Y QGAAKLVKII K APDGYQLSK, L GLPKTNTQVJ

NTRKIQVDDQ

STAITSSPAA

ISDYFTGDET

FEFQLPGNLK

HLNDSDGRGP

QAMKlDQTNPT

GDTNI(AYRLE

RNIDYDEANQ

VVGAELVEGK

GQHSEDDGTA

PNSLVVKDTT

TELDANNPAL

TWTIAVNLSN

EEPSEI<NQNT

SIQHGGESVK

TNVTEDGTIT

AAGSTDTVSN

L.AGAIFQIWD

ITIDEETSAE

PDRTNANGQL

QGSAELIKKD

PAGYLLNTEP

AKGETIQTGL

VVASDNFVSY

GTYYYKEIKA

NANPLPGTIF

PIYFVVKKNS

?ITFLNRAGEK

(EQPIALGDLI

(NLAPGTYRFV

)QQKNALAGAE

SAVAFTIAATA

N YFFVFIGLL VGLASWLFYK KSKK EF125-3 (SEQ ID NO:467) .233 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

TAACTTTO TTAC'VATCA AGGCACGG-CT AAATTA-ATCA AAAAAGATGT

GAATGGACAC

TTATTAAGTG G PTGACGACAA A~ TTTGTAGAAA C

ATTGCTGAGA

TACAAAGGGG C

GTTTTTGAAT

GATGGCAAA*

CCACCAAAAT

ATTCCTGGTG2 AAAG3'ACGCG

TTAAATTGT

AAAGATGGTT

GATGCAACGG

TCAAATGATA

GGACGTAAAG

GCCGATGAC

AAAGTTTCTG

GGGCATTACG

CATCCATI'C

ATtAATTATC

GGTGCGGTGT

ATGTCTGACA

GTGGAGACAC

ATTGCCAAAG

TACCAAGGTG

GAATTTAAAG

AACCAAGGGT

AAAGCACCGG

GCCAAAGACA

TCCAAAAAAA

CTTGGT

,TGCGACATT

~TAATCAAGG

CAAAGCGCC

LAAATGCTGG

:TTTCCAAAT

.ATATGATCA

1'TATCTTTAG

PACCAGATGG

kTTTCAA.AGG

:CGTCTI'TAA

ATCGAATCGA

CATTGGCTAT

ATGGCTATAT

AACAACCACT

TCAACGAGCA

AGAATCAGCC

AA.ATAACAAC

T'ITTAGTGGA

ATGTAACCGC

A.AGGAACTGC

TTAAGGTCAI

AGCAAGGCAP

AAGCGCCAAC

ACAACCAAGC

CTGCCAAGCIJ

TGACAGATG(

TAGTTCAAG'

ATGGTTAMC

AACCTGAAC'

CAAAACCAAk TAAAGTGcTTI

GGAAATTGTT

AACIAGGCTAT

TAAACCAGCCG

CGTGAAAACG

CAATA.AACAA

AGACTTGGCG

CGCAGATAT

TGATCCGGAG

GAAAATTGAT

AAACGGAA

GGAGGATTTA

CGTCAATAA-A

AGATGAGTTA

AGGTCAAACC

CCAAGGTTCA

GGATAAGACTl

*AACGAAAGCA

*CCAATTAGGP

TCAATTAACC

TGATGAAACP

AGTCATTGCC

TAGCTATCTJ

CAAACCAGC(

GGTGAAAAT'

AGAGACAGG(

C GAACCACTMh k AcTGTCTAA( r CGTGAATrc( N. TCAGCCAAC

GATGC:GAAG

GCAGAGCACT

TTATI'AAATA

GTCGTGCTGI

AATAGCCCAG

TCATTAGGGA

CCAGGTACCT

ATTATTTATC

ATTTTCCAAT

GCCAATr.CGA

AAATCTTTG

GGTGCTOGTA

CAACCCATT

GAGTTTCTAA

TTAGCGGGTG

CCGATAACA'I

GGCGAAATI

CCAACAGGC I

AAAGAGCAGC

*AAAGAAAACC

GGGCAAACCC

AAAAACTTAC

CTTAATGAA.2

ACTGTGGTA(

C' GATCAGC"A

CAAACTGTC(

PL CAACCACCAX 3 CAAGCTGTC, 3 GGCACGTT A. ACGAAACA

GAGAAACCAT

TAGCCCCAGG

CCACGCCAGT

CTAGTGACAA

ACCAACCATT

ITACAGCAAC

ATTATTACAA

CTGAATTAGT

TAGGGGCCTT

ACCCACTTCC

AAAGAGA-AGT

GCTATGAATT

.kTTT'TGTAGT

ATTATCAAC

CAGTTTTTC

TCTTGAATCG

ACCCTAAACG

*ATCTGTTAGA

*CAATTGCTT-1

AAACAGGTCP

TAGATGGAC;

3CACCGGGAAC

"CGCCAAGCGC

-TTAAAGCACC

k. AGALATGCCT' 3 CTCGTTICAT' P, ALATATACCT 3 CATTCACTA! 3- TTAACGAGAK C; CAGCTAGAG

TCAAACAGGC

AAAATATCC

CCCATTTGPA

CTTGTGAGT

AGCAGGTGCT

GAGTGGCAAA

AGAAATCAAA

AAAAGTAGAA

CGCCAA=TC

AGGAACGATT

AACTGCTGA-A

AGATGAACTG

GA.AGAAGAAT

AGAAGTAATG

AATTTACA-AT

TGCAGGAGAA

GCTAAATGAA

CACAACGCTA

AGGCGATcTT AGCATTrOGCA

AACCAATCTG

GTATCGT=I

AAGCTTTACG

TTTTATTIAAT

C' AGCAGGTGCT r ACGTTCTGAC r' TCTGGAAACA r TGCGGCAACA A, AcAACCTGTA A GACAGGTTGG 9 EF125-4 (SEQ ID NO:468) 99*9 9999 9 .9 0 6e 9.

NFVNYQGTAK

TTNNQGEIVA

KGAFQ IVKTN PKLPDGADY I

KLYRIENGEK

NDKQPLDELE

VSEITTDKTG

NYQGTAQLTK

ETQAPTSYLL

FKVTDAETGQ

KDKPELVNAG

LII(KDVNGHL

EHLAPGKYRF

SADQPLAGAV

IYPELVKVEI

IFEREVTAEK

FVNYQAEVMG

EIYAKGLNEG

ENETGEALAG

NETPSASFTI

TVARSLRSDN

TFVNEKQPVS

LSGATFKVLD

VETKAPTGYL

FELYDHNKQS

RGDFXGDPEI

DGSLAMEDLG-

RKVNEQGQTL

HYVLVETKAP

AVFKVIDETG

AKDNQCKPAT

QGLVQVNHLQ

KKTKPNQPTT

AKGETIQTGL

LNTTPVPFE I

LGITATSGKD)

FQLGAFANFK

AGSYELDELD

AGAVFAIYNA

TGYLLDTTH

QTVDGQTNLM

VVLKAPFIN'Y

PGKYT'JE:TY

KQA.AFETGWL

AEKNAGKPAV

GKI IFRDLAP

GRAVFKKIDA

ATDGYIVNKQ

DEQNQPQGSP

PFDVTAQLGK

SDKQGKV lAK

QCAAI(LVKID

APDGYQLSKQ

VVASDNFVSY

GTyyyKEIKA

NANPLPGTIF

P IYFVVKKNS

ITFLNRAGEK

EQPIALGDLI

NLAPGTYRFV

QQKNALAGAE

AVAFT IAATA EF126-1 (SEQ.ID.NO:469)

TAGCGAAAGA

ATGGTGATTG

GAAGCGGTTA

AGTCTGGCTG

GAAACGGAAC

AAATAGGGAG

TCGCTGGAAC

AAGCAGGAGA

ATTGTAAACG

CAGTCGATT

GATTAAAATG TTTAAGAAAG AGTTGTGGGA AATFTCAGTC TACAGAAGGA ATGACCAATA GATATTGGAA GGACAAGCTA AGTAGTTGTT GAAGATGCTA

CAACGAAAT

CCAC-ATrGC

CGGTGAAAGT

CT'rTCCCAGT

GTGGTAGTTT'

ATTATCGACA

TTTrAGCTGAA

GAAAGACGAC

TCAAGCGGGT

'FrcAGATAAT 234 TABLE 1. Nucleofide and Amino Acid Seqeuenccs of E. faecalis Genes.

TTTCCACATG

GTGATGCTGG

ATTA.ATI'CAG

AGCCAATTTG

GGATTGAAAC

ACGTA=TCC

AAGACCAATA

TCAGTGGAAT

GAAkATTACTA

AGTTCAGTGA

CAGTTGCAAC

TTTACAACCC

GCT'rCATTAA

GGAAATGATG

TACGTAGGAA

ACCCTTGTAA

ACGATTCCTA

ACAAAAGATA

TGGCA L'CTCA

GATGACATTA

CATCT'ACAG

AAACAAGCAG

AAAATTAAAA

cccAAccALAG

ACCGTA-ACAC

TTAGATTTAP

GAAACAAC

ACTGATGTAJ

CAAGAAAATI

AGTGGTCAT)

GAATTACCA(

AACGAAGGTC

GAAGATCCA

GACCAAGAAW

CAAGCCAGC,

GANGAAAAT(

ACFTITACT,

ATGACTATI'.

GAACAAGGC'

CATTCCAAC.

GAACCTAAA

ACGAACCCA

ACAACAGTA

TI'TCGCATA

TAAGACAAGC

CTTCATATCG

CTGATTATGA

TCTCTGGTTT

TCCTAGA

TATTACTGAC

CCAATGA'TTC

ATAGTAATGA

ACCAAGGCTA

ATTCATACTI'

AAGGGTCTAG

AATTAAAACA

CGATTGCCAA

TGCCTGI'CA

ACATCACCAT

GTAGTGGGAC

AAAATGACAA

TCGAAAATCA

AAACAGCCTT

ATAAAGTGJCI

CTAACCGCAC

ACAGCTATGP.

CTGACGCAAC

CCGACTTAAP

CACCGCCAGI

CCAACCGCG;

CTI'GGACCCI

AAGTCACAGI

k. ACAAAGTAAC k CGTACACAW

'CTI'ATAFTTQ

ACGTCTTGC

k. CGATTACAA r TTAAATGGA.

k. TG.GTGGATG, 3 GCAAAGATG' P, TCAACAAAA A CCACTAAAA 3 GGATTCCCA A AGCCAACCG C AACCGCTAA G TCAATTTTG A ATCCACTT I A CAAAAAATP

GATTGATGAA

CGCGAAAA

TATGAATGTG

TGGAGACGTT

TACGTACAAT

AGATGGGGTC

AATCAATGAA

CTACCAAGGT

TGAAATGATT

TGATAAATAT

CACACCAGAA

AATTCTCAAA

TCAATTTGAT

AATTAACGGA

TCACTACGAA

AATGAATCAA

TGCGCATGCG

AGAACAC'ITA

TGGCAACGA-A

AGATATCATT

ACTAACACAA

CTATTTAAG'I

GGACGAAGAA

CTTTCGCAAI

TGATCCAAA1

TCAAGAATTI

AGCCAGCATC

TGAAAATG9

TTTTGAAATC

r GACCA~rACI

ACAACGTCG(

k. TTCCAACAA6 k. AGATATCGAX N. CGTCAAAAC, A. CA ITAATAA T TACAGATAA' A AGATGACAG, T TAAAACTGA A CCAAGCCG-A T AACACCGCC A ACCGAAAAA ,G AAAATCAGC 'A CATGATCGC .A AAAAAGAAA

GTGGTTCAAG

CAA-TTTATGT

CGCGTCAATA

CGGACGTATG

CAAACACACG

CCTAATACAC

TATCCAGATC

GCAGCAGCAG

AATCTA'T

AAAACAGAAG

GATTTTATTA

GATCGTCTCG

ATTCAATCTG

CAAACCATT

CTCAAAGAAA

GGAACAATTG

TGTGACGTGA

GATTTAACCA

ACCAGTACT'I

GATGTGAAAC

GAAAkATAAC,' GGTCATACG9

LTTAGCGCCTJ

GALAGGTGAC(

ALWGCTAAA(

*AA.ATGGAAC(

GTAGATGACJ

I AAAGATGTT,

AACAANCAA,

C' ACTAAAATC.

-ATTCCCAAC

'CCAACCGTA

k. GGCCAAGAA k. GCT'ITCGGT PL GTGTrAGAC T' GGCATAGTA C TACTCTTAC C GCAACGGAE C rTAAACTrI T GCACCAACC A CCGTI'GACC GCTTATCTGA T TCCTGATGG A CGCAATTGAC GTGGTACGCC A.

GAGATTAAC G.

GTrTAGA"CC F CAAGACATCC T A.AGTDTTAGC G GGGAAAGTG'1 T TGGGT!CCTIT T

CAAGCCAATCT

CGCAATCCAC A

CGACCGCTAC

CAGCAACTAG

ATACAGCGATI

CTAAGGAATI' T CGCCAGAAGA T ATCGTGAAGA I1 GGACCCAAGC C TCACCGACGA

AAGTAACTTT

ATACA.ATGAC

'ACATTGAACA

3TGTTACATTC 3ACrTAGAAG 3TCAAAACAGC k. 'TAATAA.AGT

CAGCTAACCG

3 CNGACAGCTA A. AAGCTAGCGC C AAGCCGACTT A CACCACCTC C ATTAGATTT A ACGAAACAAG A TCACAGACGT A CACAAGAAAA T TAGCTGGTCA 103 AAGAATTAGC

GCAACGAAGG

;C CAGAAGACCC ;C CTACAAATCA

AAGACCGC

P.AGACAAAA

rATGATAAA

PCCGCCCCA

PATCGA.AAA

TACTTGCAT

CTTCAAGTC

T'rAAACCAA

CAATC=IA

GTAAAACAA

ATTGATGAT

CCAGCAACA

GACGATGCT

'ACAGAAGGT

rCATGCAGCA

~CCAGAAGCG

CCAACGATT

'AGTTTCGAT

AGCATCG'I'

LkATGGTAAA

GAAATGAAC

E'ATCACCACT

%.GGCGGGATT

CAACAAACCA

P.CAAGAACAT

rTTcCGGTAAC

GTTAGACATC

CAAAGTAACA

TGACTATTTA

AACGGACGAA

GAACTTTGGC

ACCAACGCCA

A.ACCAACCGT

CACATGGACC

GAAAGTTNCT

TAACAAAGTA

TACATACACA

GCCcTATATT TGACGTG ETG

AAAAAAACCT

TCAACCACCA

AATGACTAAT

GGCTA'FTAGC

::66.

AGTAAAGGAA TTCATTTACC GGTTTAATTG TCCTTATAGT

AATTAG

EF126-2 (SEQ ID NO:470) MF KXATKLLSTM VIVAGTVVGN FSPThALAEE AvKAGDTEGm TNTVKVKDDS LADCKRILEG QATFPVQAGE TEPVDLVVVE DASGSFSDN-F PHVRQAIDEV VQGLSDQDRV MLASYRGGKQ FMFPDGKTKI NSADYDMNVR VNTQLTYDKS QFVSCFGDVR TYGGTPTAPG LKL.ALDTYNQ THGDLTNRKT YFLLVTDGVA NTRLDGYLHK TNTNDS INEY PDPRHPLQVS VEYSNDYQGA AAVrALNQE ITNQGYEMIN AYWESVESLS SVNSYFDKYK TEVGPFVKQE LQQCSSTPED FITSQSIDDF TTQLKQIVKD RLAQSTPATA SLTIANQFDI QSATATDDAG NDVPVQINGQ TISATSTEGY VGNITIHYE-V KENTAIDAAT LVS SGTMNQG TIAKEFPE-AT 235 TABLE 1. Nucleotide and Amino Acid Seqeuences of~ E. faecalis Genes.

I PKNDNAHAC

DINKVLDIID

IKTDATDEEL

DLTNRDQEFK

ENN1VTFE2

EGDVLHSNKP

ASMVDDINKV

TITTKIKTDA

PKQPLKPKKP

DVTPEDPTIT

VKVTDENGKD

APYIEQGGIP

WNVKTAFGNE

XQADSYDYLS

TVTPPAPTPE

LDITDVKVXX

TDEELAPYIE

LTPTNHQAPT

KDIENQEHLD

VTANGTVTQE

NQADLNFGNE-

TSTWTQASMV

GHTYTMTITT

OPTITKDIEG

ENGKDVTDNG

QGGIPNQADL

NPVNFGKSAS

LTNREDSFDW

NNKVTFEK

GDVLHSNKPT

DDINXVLDIT

KIKASATDEE

QEHLDLTNRD

IVTQENLZVT

NFGNEGDVLH

KGIHLPM'TNT

HVKTAPGNET

QADSYDYLSG

VTPPPVDPNI

DVKVTDENCK

LAPY IEQGG I

QEFKWNVKTA

FTMNKRDDSY

SNKPTVTPPA

TVNPLYMIAG

STWTQASMVD

HTYTMTITTK

AKDVEGQEHL

DVTANGKVTQ

PNQADLNFGN

FGNETSTWTQ

SYLAGHTYTM

PTPEDPKKPE

LIVLIVAISF

G ITKNKXRKN EF126-3 (SEQ ID NO:471) 0* S S

S.

S S *5

S.

S S

S.

S S

S

S.

S S *55 S

S

S

TGAA

GAAGCGGTTA

AGTCTGGCTG

GAAACGGAAC

TTTCCACATG

GTGATGCrG

ATTAATTCAG

AGCCAATTTG

GGATTGAAAC

ACGTATTTCC

AAGACCAATA

TCAGTGGAAT

GAAATrACTA

AGTI'CAGTGA

GAGTTGCAAC

TTTACAACCC

GCTTCA'rTAA

GGAAATGATG

TACGTAGGAA

AAGCAGGAGA

ATTGTAAACG

CACTCGA'ITT

TAAGACAAGC

CTTCATATCG

CTGATTATGA

TCTCTGG'I=

TCGC ITAGA

TATTAGTGAC

CCAATGATTC

ATAGTAATGA

ACCAAGGCTA

ATTCATACTT

AAGGGTCTAG

AATTAAAACA

CGATTGCCAA

TGCCTGTTCA

ACATCACGAT

TACAGAAGGA

GATATTGGAA

AGTAGTTGTT

GATTGATGAA

CGGCGGAAAA

TATGAATGTG

TGGAGACGTT

TACGTACAAT

AGATGGGGTC

AATCAATGAA

CTACCAAGGT

TGAAATGATT

TGATAAATAT

CACACCAGAA

AATTGTCAAA

TCAMTTTGA7

AATTAACGGA

TCACTACGAA

ATGACCAATA

GGACAAGCTA

GAAGAMCTA

GTGGTTCAAG

CAATTTATGT

CGCGTCAATA

CGGACGTATG

CA.AACACACG

GCTAATACAC

TATCCAGATC

GCAGCAGCAG

AATGCGTATT

AAAACAGAAG

GAT'MlATTA

GATCGTCTGG

ATTCAATCTG

CAAACCATIT

GTCAAAGAAA

CGGTGAAACT

C'TTCCCAGT

GTG3GTAGTTT

GCTTATCTCGA

TTCCTGATGG

CGCAATTGAC

GTGGTACGCC

GAGATTTAAC

GTTTAGATGG

CAAGACATCC

AAGI=AC

GGGAAAGTGT

TGGGTCCTTI'

CAAGCCAATC

CGCAAkTCGAC

CGACCCTAC

CAGCAACTAG

ATACAGCGAT

GAAAGACGAC

rCA-AGCGGGT

TTCAGATAAT

TCAAGACCGC

AAAGACA.AAA

TTATGATAAA

AACCGCCCCA

GAATCGAAAA

7rIACTTGCAT

TCTTCAACTC

GTTAAACCAA

TGAATCTTI'A

TGTAAAACAA

TA ETGATGAT

ACCAGCAACA

GGACGATGCT

TACAGAAGGT

TGAT

EF126-4 (SEQ ID NO:472) EE AVKACDTEGM TNTVKVKDDS LADCKRILEG QATFPVQAGE TEPVDLVVVE MLASYRGGKQ FMFPDGKTKI NSADYDMNVR LKLALDTYNQ THGDLTNRKT YFLLVTDGVA so*. VEYSNDYQGA AAEVLALNQE ITNQGYEMIN LQQGSSTPED FITSQSIDDF TTQLKQIVKD 8: NDVPVQINCQ TISATSTEGY VGNITIHYEV EF127-1 (SEQ ID NO:473)

DASCSFSDNF

VNTQL'IYDKS

NTRLDGYLHK

AYWESVESLS

RLAQSTPATA

KENTAID

PHVRQAIDEV

QFVSGFGDVR

TNTDS INEY

SVNSYFDKYK

SLTIANQFDI

VQGLSDQDRV

TYGGTPTAPG

PDPRHPLQVS

TEVGPFVKQE

QSATATDDAG

TAGCGAAAGA

ATGGTGATTG

GAAGCGGTA

AGTCTGCCTG

GAAACGGAAC

TTTCCACATG

GTGATGCTGG

ATTAATTCAG

AAATAGGGAG

TCGCTGGAAC

AAGCAGGAGA

ATTCTAAACG

CAGTCGAI'T

TAAGA'.AAGC

CTTCATATCG

CTGATTAGA

GATTAAA.ATG

AGTTGTGGGA

TACAGAAGGA

GATATTGGAA

AGTAGTI'CTT

GATTGATGAA

CGCGAAAA

TATGAATGTG

TTTAAGAAAG

AATITCAGTC

ATIGACC1AATA

GGACAAGCTA

GAAGATGCTA

GTGG1rCA-AG CAATITATr.T

CAACGAAATT

CC-ACATTGGC

CGGTGAAAGT

CTT'ccCAGT

GTGGTAGTT

GCTTATCTGA

'ITCCTGATGG

ATTATCGACA

'ITTAGcTG.A

GAAAGACGAC

TCA.AGCGGGT

TTCAGATAAT

TCAAGACCGC

AAAGACAAAA

TTATGATAAA

CrGCCTCAATA CGCAATTGAC 236 TABLE I1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

AGCCAAITTTG

GGATTGAAAC

ACGTATTI'CC

AAGACCAATA

TCAGTGGAAT

GAAATI'ACTA

AGTTCAGTGA

GAGTTGCAAC

GGAAATGATG

TACGTAGGAA

ACCCTTGTAA

ACGATTCCTA

ACAAAAGATA

TGGCATGTCA

GATGACATTA

GATGTI'ACAG

AAACAAGCAG

AAAATTAAAA

CCCAACCAAG

ACCGTAACAC

TAGATTTAA

GAAACAAGCA

ACTGATGTAA

CAAGAAAATA

AGTGGTCATA

GAATTAGCAC

AACGAAGCTG

GAAGATCCAA

GACCAAGAAT

CAACCCAGCA

GAN'GAAA.ATG

ACTT=ACTP.

ATGACTTT,

GA.ACAAGCCC

CATTCCAAC;

GAACCTAPLAC

ACGAACCCAC

ACAACAGTA2 T'FrGGCATAJ

TCTCTGGTTT

TCGCTTTAGA

TATTAGTGAC

CCAATCATTC

ATAGTAATGA

ACCAAGGCTA

ATTCATACTT

AAGGGTCTAG

AATTAAAACA

CGATTGCCAA

TGCCTGTTCA

ACATCACGAT

GTAGTGGGAC

AAAATACAA

TCGAAAATCA

AAACAGCCTIT

ATAAAGTGCT

CTAACGGCAC

ACAGCTATGA

CTCACGCAAC

CCGACTTAA.A

CACCGCCAGT

CCAACCGCGA

CTTGGACCCA

AAGTCACAGA

ACAAAGTAAC

CGTACACAAI

CTIATATTGP

ACGTG.TTGCP

CGATTACAAJ

TTAAATGGA;

TGGTGGATGZ

GCAA-AGATG=

TGAACAAAAJ

CCACTAAAA'

GGATTCCMA

AGCCAACCG'

AACCGCTAAL

TCAATTITG

~ATCCACTTT.

~CAAAAAATA

TGGAGACGTT

TACGTACAAT

AGATGGGGTC

AATCAATGAA

CTACCAAGGT

TGAAATGATTI

TGATAA.ATAT

CACACCAGAA

AATTGTCAAA

TCAArlTTGAT

AATTAACGGA

TCACTACGAA

AATGALATCAA

TGCGCATGCG

AGAACALCTTA

TGGCAACGAA

AGATATCATT

AGTAACACA-A

CTATTTAAGT

GGACGAAGAA

CTTTGGCAAT

TGATCCAAAT

TCAAGA.ATT

*AGCCAGCATC

TGAAAATGG'I

TTTTGA.AATC

*GACCATTAC'I

ACAAGGTGGC

LTTCCAACAA;

AGATATCGAJ

CGTCAAAACI

CATTAATAA3

STACAGATAA'

SAGATGACAC

[TAAAACTGA(

~CCAAGCCGA(

r' AACACCGCC' k~ ACCGAAAAAL

SAAAATCAGC.

~CATGATCGC.

A~ AAAAAGAAA

CGGACGTATG

CAAACAkCACG

GCTAATACAC

TATCCAGATC

GCAGCAGCAG

AATGCGTAT

AAAACAGAAG

GAT=TATTA

GATCGTCTGG

ATTCAATCT'G

CA.A.ACCATTT

GTCAAAGAAA

GGAACAA=

TG~TCACGTGA

GAT=;AACCA

ACCAGTACTT

CATGTGAAAG

GAAAATA.ACA

GGTCATACGI

11'AGCGCCTI

GAAGGTGACC

ATTGCTAAAC

AAATGGAACC

GTAGATGAC;

AAAGATGTT;

AACAANCAAC

ACTAAAATCI

ATrCCCAACC

CCAACCGTAJ

kGGCCAAGAA( k GCTTTCGGT k GTGTTAGACA r' CGCATAGTA,

:TACTCTTAC

:GCAACGGAT

ZTTAAACTTT

r GCACCAACG P, CCGTTGACG A. AGTAAAGGA A GG IMAA77 A AA TAG GTIGTACGCC A.

GAGATTTAAC G.

GTI'TAGATGG T CAAGACATCC T AAGTTTTAGC G GGGAAAGTGT T TCGGTCCTTT T CAAGCCAATC T CGCAATCGAC A CGACCGCTAC G CAGCAACTAG Ti

ATACAGCGAT

CTAAGGAATT 'I CGCCA6AAGA 9 ATCGTGA.AGA 91 GGACCCAAGC C TCACCGACGA

AAGTAACTTT

ATACAATGAC

ACArDTGAACA

TGTTACATTC

ACGTAGAAG

TCAAAACAGC

TTAATAALAGT

CAGCTAACGG

CNGACAGCTA

kAAGCTAGCGC

AACCCACTT

k CACCACCTGC

"ATTTAGATTT

ACGAAACAAG

k TCACAGACGT k. CACAAGAAA.A r TAGCTGGTCA S AAGAATTAGC G GCAACGA.AGG C CAGAAGACCC C CTACAAATCA A TTCATTTACC G TCCTTATAGT

ACCGCCCCA

AATCGAAAA

TACTTGCAT

CTTCAAGTC

TA.A.ACCAA

GAATCT'TA

GTAAAACAA

'ATTGATGAT

CCAGCAACA

GACGATGCT

ACAGALAGGT

C-ATGCAGCA

CCAGAAGCG

~CCAACGATT

'AGTTTCGAT

:AGCATGGTG

kAATGGTAA.A

LGAAATGAAC

E'ATCACCACT

kGGCGGGATT

CAACAAACCA

A.CAAGAACAT

TTTCGGTAAC

CTTAGACATC

CAAAGTAACA

TGACTATTTA

AACGGACGAA

GAACTTTGGC

ACCAACGCCA

AACCAACCGT

CACATGGACC

GAAAGTTNCT

TAACAAAGTA

TACATACACA

GCCTTATATT

TGACGTGTTG

AAA.AAAACCT

TCAAGCACCA

AATGACTAAT

GGCTATTAGC

0O *00* 0 @0 0 0 0 .0 E7127-2 (SEQ ID NO:474) 14F KKATKLLSTM VIVAGTVVGN FSPTLAIAEE, AVKAGDTEGM TNTVKVKDDS LADCKRILEG QATFPVQACE TEPVDLVVVE DASGSFSDNF PHVRQAIDEV VQ( MLASYRGGKQ FMFPDGKTKI NSADYDMNVR VNTQLTYDKS QFVSGFGDVR TYC LKLALDTYNQ THGDLTNRKT YFLLVTDGVA NTRLDGYLHI( TNTNDSINEY PD' VEYSNDYQGA AAEVL.ALNQE ITNQCYEMIN AYWESVESLS SVNSYFDKYK TZ LQQGSSTPED FITSQSIDDF 7rQLKQMVD RLAQSTPATA SLTIANQFDI QS.

NDVPVQINGQ TISATSTEGY VGNITIHYEV KEN'rAIDAAT LVSSGTMNQG TI IPKNDNAHAC DVTPEDPTIT KDIENQEHLD LTNREDSFDW HVKTAFGNET ST DINICVLDIID VKVTDENGKD VTANCTVTQE NN TF24N1( QADSYDYLSG HT IKTDATDEEL APYXEOGGIP NQADL.NFGNE GDVLHSNKPT VTPPPVDPNI AK

GLSDQDRV

GGTPTAPG

PRHPLQVS

GPFVKQE

ATATDDAG

AKEFPEAT

WTQASMVD

YTMTITTK

DVEGQEHL

TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

*DLTWRDQEFK WNVKTAFGNE ENNKVTFEMrN XQADSYDYLS ECDVLHSNKP TVTPPAPTPE ASMVDD INKV LDITDVKVXX TIFI'KIKTDA TDEELAPYIE PKQPLKPKKP LTP1'NHQAPT G ITKNKKRXN

TSTWTQASMV

GHTYTMTITT

DPTITKDIEG

E-NGKDVTDNG

QGG?--NQACL

NPVNTGKSAS

DDINKVLDIT

K IKASATDEE

QEH-LDLTNRD

IVTQENNKVT

NFGNEGDVLI-i

KGIHLPMT-NT

DVKVTDENGK

LAPY IEQGG I

QEFK!RNVKTA

FTMKKDOSY

SNKPTVTPPA

TVNP LYM lAG

DVTANGKVTQ

PNQADLNFGN

FGNETSTWTQ

SYLAGHTYTM

PTPEDPKKPE

LIVLIVAISF

EF127-3 (SEQ ID GAATCAA GGAACAATTG CTAAGAATT TCCAGAAGCG

ACGATTCCTA

ACAAAAGATA

TGGCATGTCA

GATGACATTA

GATGTTACAG

AAACAAGCAG

AAAATTAAAA

CCCAACCAAG

ACCGTAACAC

TTAGATTTAA

AAAATGACA

TCGAAAATCA

AAACAGCCTT

ATAAAGTGCT

CTAACGGCAC

ACAGCTATGA

C~rACGCAAC

CCGACTTAAA

CACCGCCAGT

CCAACCGCGA

TGCGCATGCG

AGAACACTTA

TGGCAACGAA

AGATATCATT

AGTA.ACACAA

CTATTTA-AGT

GGACGAAGAA

CTTTGGCAAT

TGATCCAAAT

TCAAGAATI'T

AGCCAGCATG

TGTGACGTGA

GATTTAACCA

ACCAGTACT

GAT'TGAAAG

GAAAATAACA

GGTCATACGT

TTAGCGCCTT

GAAGGTGACG

ATTGCTAALAG

AAATGGAACG

GTAGATGACA

CGCCAGAAGA

ATCGTbA.AGA

GGACCCAAGC

TCACCGACGA

AkAGTA.ACTTT

ATACAATGAC

ACATTGA-ACA

TGTTACATTC

ACGTAGAAGG

TCAAAACAC

TTAAT

TCCAACGATT

TAGTTTCGAT

CAGCATGGTG

AAATGGTAAA

TGAAATGAAC

TATCACCACT

AGGCGGGATT

C AAC AAACC A

ACAAGAACAT

TTTCGGTAAC

0 00 GAAkACACA CTTGGACCCA EF127-4 (SEQ ID NO:476) NQG TIAXEFPEAT IPKNDNAHAC DVTPEDPTIT DINKVLD 110 VKVTDENGKD IKTDATDEEL APYIEQGGI P DLTNRDQEFK WNVK<TAFGNE.

KEDIENQEHLD

VTAINGTVTQE

NQADLNFGNE

TSTW.TQASMV

LTNREDSFD)W HVKTAFGNET NNXVTFEMNK QADSYDYLSG GDVLHSNKPT VTPPPVDPNI

DOIN

STWTQASMVD

HTYTMTITTK

AKDVEGQEHL

EF128-1 (SEQ ID NO:477) a a a

TAGCGAAAGA

ATGGTGATTG

GAAGCGGTTA

AGTCTGGCTG

GAAACGGAAC

TFI'CCACATG

GTGATGCTGG

ATTAATTCAG

AGCCAATTTG

GGATTGAAAC

ACGTATTTCC

AAGACCAATA

TCAGTGGAAT

GAA.ATTACTA

AGTTCAGTGA

GAGTTGCAAC

TT"TACAACCC

GCT'TCATTAA

GGAAATrGATG

AAATAGGGAG

TCGCTGGAAC

AAGCAGGAGA

ATTGTAAACG

CAGTC GATT

TAAGACAAGC

CTTCATATCG

CTGATTATGA

TCTCTGGTTT

TCGCTTTAGA

TATTAOTGAC

CCAATGATTC

ATAGTAATGA

ACCAAGGCTA

ATTCATAC~TT

AAGGGTCTAG

AATTAAAACA

CGATTGCCAA

TGCCTrGITCA

GATTAAAATG

AGTTGTGGGA

rACAGAAGGA

GATATTGGA

AGTAGTTGTT

GATTATIGAA

CGGCGCAAAA

TATGAkTGTG

TGGAGACGTT

TACGTACAAT.

AGATGGGGTC

AATCAATGAA

CTACCAAGGT

TGAAATGATT

TGATAAATAT

CACACCAGAA

A.ATTGTCAAA

TCAATTTGAT

AATTAACGGA

TTTAAGAAAG

AATI'TCAGTC

ATGACCAATA

GGACAAGCTA

GAAGATGCTA

GTGGTITCAAG

CAATTITATiGT

CGCGTCAATA

CGGACGTATG

CAAACACACG

GCTAATACAC

TATCCAGATC

GCAGCACCAG

AATGCGI'AT

AAAACAGAAG

GATTATTA

GATCGTCTGG

A1'TCAATCTG

CAAACCAT

CAACGAAATT ATTATCGACA

CCACATTGGC

cGGTGAAACT

CTTI'CCCAGT

GTGGTAGTTT

GC LTATCTGA

TTCCTGATGG

CGcAATTGAC

GTGGTACGCC

GAGATTTAAC

G'ITAGATG

CAAGACATCC

A.AGT'TTAGC

GGGAAAGTGT

TGGGTCC=I

CAAGCCAATC

CGCAATCGAC

CGACCGCTAC

CAGCAACTAG

TTTAGCTGAA

GAAAGACCAC

TCAAGCGGGT

TTC AGATAAT

TCAAGACCGC

AAAGACAAA-A

'ITATGATAAA

AACCGCCCCA

GAATCGAAAA

TTACTTGCAT

TcTTCAAGTC

GTTAAACCAA

EYGAATCTTTA

T'TAAAACAA

TAT~cATGAT AccACCAACA

GGACGATGCT

TAcAGAAGGT 238 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faeculis Genes.

TACGTAGGAA

ACATCACGAT

ACCCTTGTAA

GTAGTGGGAC

ACGATTCCTA AAAATGACA-A ACAAAAGATA TCGAAAATCA TGGCATGTCA

AAACAGCCTT

GATGACATTA

ATAAAGTGCT

GATGTTACAG CTAACGGCAC AAACAAGCAG ACAGCTATGA AAAATTAAAA CTGACGCAAC CCCAACCAAG CCGACTTAAA ACCGTAACAC CACCGCCAGT TTAGATFTAA CCAACCGCGA GAAACAAGCA CTTGGACCCA ACTGATGTAA AAGTCACAGA CAAGAAAATA ACAAAGTAAC AGTGGr-TCATA CGTACACALAI GAATTAGCAC CTTATATTGP AACGAArGGTG ACGTGTTCZ.

GAAGATCCAA

CGATTACAAJ

GACCAAGAAT

TTAAATGGMI

CAAGCCAGCA TGGTGGATGI *GANGAAAATG GCAAAGATGr ACTTTACTA TGAACAAAA *ATGACTATTA

CCACTAAAA'

*GAACAAGGC GGATTCCCAk **CATTCCAACA

AGCCAACCG

*:GAACCTAAAC

AACCGCTAA

ACGAACCCAC TCAATTTTG *ACAACAGTAA

ATCCACTT

TTTGG-CATAA CAAAAAATA

TCACTACGAA

AATGAATCAA

TGCGCATGCG

AGAACACTTA

TGGCAACGAA

AGATATCATT

AGTAACACAA

CTATAAGT

GGACGAAGALA

CTTTGGCAAT

TGATCCAA.AT

TCAAGAATTT

AGCCAGCATG

TGAAAATGGT

TTTTGAAATG

GACCATTACT

ACAAGGTGGC

TTCCAACAAA

AGATATCGA-P

SCGTCA-AAACP

~CATTAATAM;

r~ TACAGATAk) 5, AGATG-ACAGC r TAAAACTGAC N. CCAAGCCGA( T AACACCGCC' A ACCGAAAAAk G AAAATCAGC.

A CATGATCGC A AAAAAGA.AA

GTCAAAGAAA

GGAACAATTG

TGTGACGTGA

GA'TTTAACCA

ACCAGTACTT

GATGTGAAAG

GAAAATAACA

GGTCATACGT

TTAGCGCCTT

GALAGGTGACG

ATTGCTAAAG

AAATG;GA.ACG

GTAGATGACA

APLAGATGTTA

AACAANCAAG

ACTAAAATC.A

ATCCCAACC

CCAACCGTAP

GGCCAAGAAC

GCTTTCGG2;

GTGTTAGACI

7GGCATAGTA2

TACTCTTAC'.

GCAACGGAD

TTAAACTTrD r GCACCAACGi kL CCGTTGACG PL AGTAAAGGA A GG TrAATT A A.ATTAG ATACAGCGAT TGATGCAGCA CTAAGGAATT T CGCCAGAAGA T

ATCGTGAAGAI

GGACCCAAGC C

TCACCCACGA

AAGTAAC=T I~ ATACA.ATGAC I ACATTGAACA

TGTTACAITCC

ACGTAGAAGG

TCAkAACAGC

TTAATAAAGT

CAGCTAACGG

CNGACAGCTA

AAGCTAGCGC

*AAGCCGACTT

CACCACCTGC

*ATTTAGATTT

k. ACGAAACAAG k TCACAGACGT k. CACAAGAAAA r~ TAGCTGGTCA 3AA:GAATTAGC G; CAACGAAGG

CCAGAAGACCC

C. CTACAAATCA A TTCATTTACC C; TCCTTATAGT

'CCAACGATT

'AGTTTCGAT

AGCATGGTG

LAATGGTAAA

~GAAATGAAC

~ATCACCACT

kGGCGGGATT

:AACAAACCA

kCAAGAACAT rTTCGGTAAC

GTTAGACATC

CA.AAGTAACA

TGACTATTA

AACGGACGAA

GAACTTT GGC

ACCAACGCCA

AACCAACCCT

CACATGGACC

GAAAG TNCT

TAACAAAGTA

TACATACACA

GCCTTATATT

TGACGTGTTG

AAAAAAACCT

TCAAGCACCA

AATGACTAAT

GGCTATTAGC

EF1.28-2 (SEQ ID NO:478) mrVhqI'LT.TM VIVACTVVCN FSPTLALAEE AVKAGDTEGM TNTVKVKD

DASGSFSD&F

LJADCKRILEG

MLASYRGGKQ

LKLALDTYNQ

VEYSNDYQGA

LQQGSSTPED

ND VP VQINGQ

IPKNDNA.HAC

DINKVLDIID

IKTDATDEEL

D)LTNRDQEFK

ENNKVTFEMN

EGDVLHStNKP

ASMVDDINKV

TIT'1(IKTDA

PKQPLKPKKP

QATFPVQAGE

FMFPDGKTKI

THGDLTNRKT

AAEVLALNQE

FITSQSIDDF

TIsATSTEGY DVTPv-DPTIT

VKVTDEN'GKD

APYIEQGGIP

WNVKTAFGNE

XQADSYDYIJS

TVTPPAPTPE

LDITDVK(VXX

TDEELAPYI E

LTPTNI{QAPI

TEPVDLVV'JE

NSADYDmNVR

YFLLVTDGVA

ITNQGYEMIN

TrQL.KQIVKD

VGNITIHYEV

KDIENQEHLD

VTANGTVTQE

NQADLNFGNE

TSTWTQASMV

GHTYTMTITT

DPTITKDIEG

ENGY(DVTDNG

QGGIPNQADL

NPVNFGKSAS

VNTQLTYDKS

NTRLDGYLHK

AYWESVE-SLS

P-LAQSTPATA

KENTAIDAAT

LTNREDSFDW

NNKVTFENK

GDVT-HSNKPT

DDINKVLDIT

KIKASATDEE

QEHLDLTNRD

IVTQENNKVT

NFGNEGDVLH

KGIHLPMTNT

QFVSGFGDVR

TNTNDSINEY

SVNSYFDKYK

SLTIANQFDI

LVSSGTMNQG

HVrKTAFGNET

QADSYDYLSG

VTPPPVDPNI

DVKVTDENGK

LAPYIEQGGI

QEFKWNVKTA

pFTMNI(DDSY

SNKPTVTPPA

'IVNPLYMIAG

JQGLSDQDRV

rYGGTPTAPG

PDPRHPLQVS

TEVGPFVKQE

QSATATDDAG

T IAXEFP EAT

STWTQASMVD

HTyTMTITTK

AYCDVEGQEHL

DVTANGKVTQ

PNQADLNFGN

FGNETSrtWTQ SYLAGHTYTm

PTPEDPKXPE

LIVLIVAISF

GITKNKXRKN

EF128-3 (SEQ ID NO:479) 239 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

AGA TGAA.AATGGT AAAGATGTTA CAGCTAACGG

CAAAGT~

CAAGAAAATA

AGTGGTCATA

GAATTAGCAC

AACGAAGGTG

GAAGATCCAA

GACCAAGAAT

CAAGCCACCA

CANGAAAATG

ACTTTTACTA

ATGACTATTA

GAACAAGGCG

CATTCCAACA

GAACCTA.AAC

ACGAACCCAG

ACAAACTAAC

CGTACACAAT

C'ITATATTGA

ACGTrTTGCA

CGATTACAAA

TTAAATGGAA

TGGTGGATGA

GCAA.AGATGT

TGAACAAAAA

CCACTAAAAT

GGATTCCCAA

AG CCAACCGT

AACCGCTAAA

TCAATTTTGG

T~IlGAAATG

GACCATTACT

ACAAGGTGGC

TTCCAACAAA

AGATATCGAA

CGTCAAAACA

CA'ITAATAAA

TACAGATAAT

AGATGACAGC

TAAAACTGAC

CCAACCCGAC

AACACCGCCT

ACCGAAAAAA

AAAATCAGCA

ACTAAAATCA

Arl'CCCAACC

CCAACCGTAA

GGCCAAGAAC

GCTTTcGGTA

GTGTTAGACA

GCCATAGTAA

TACTCTTACT

GCAACGGATG

;i'I.AACTTTG

GCACCAACGC

CCGTI'CACGC

LACA'

CNGACAGCTA

AAGCTAGCGC

AAGCCGACTT

CACCACCTGC

ATTAGATTT

ACGAAACAAG

TCACAGACGT

CACAAGAAAA

TAGCT.GGTCA

AAGAATTAGC

GCAACGAAGG

CAGAAGACCC

CTACAAATCA

TGACTA=TA

AACGGACGA.A

GAACTTTGGC

AC CAACGC CA

AACCAACCGT

CACATGGACC

GAAAGTTNCT

TAACAAAGTA

TACATACACA

GCCTTATATT

TGACGTGTTG

AAAAAAACCT

TCAAGCACCA

AGTAAAGGA-A TTCAT EF128-4 (SEQ ID NO:480) DENGI( DVTANGKVTQ ENNKVTFEMN

XQADSYIDYLS

EGDVLHSNKP

TVTPPAPTPE

ASNVDDINKV LDITOVKVXX TITTKIKTDA

TDEELAPYIE

PKQPLKP~kP

LTPTNHQAPT

GHTYTMT ITT

DPTITKDIEG

ENGKDVTDNG

QGGIPNQA.DL

NPVNFGKSAS

KIKASATDEE LAPYIEQGGI QEHLDLTNRD QEFKWiNVKTA IVTQENNKVT FTMThKKDDSY NFGNEGD)VLH sNKPTVTPPA

KGIH

PNQADLNFGN

FGNETSTWTQ

syLAG1XTYTM

PTPEDPKKPE

EF129-1 (SEQ ID NQ:481)

TGACAAGTGA

ATTGCTACCC

CAAGAAATI'

GAAGCAGAAG

CTAAAAGCAA

AAACGTAACG

ACAATGCTAG

GCTGTTTCAA

CAAGCCGITG

GAAGCTGAAT

ATGAAAGCT?

AAACAAA.AAG

GCTGAAAAAG

CCAGTTGCCT

GAATCAAGCA

GAAAATACTG

GGAAATAATG

GCGCC ITCTG

CGTCCAGTAG

GAAGCAGGTG

TGGGCGCCAG

TCAGGAAGCG

TACTCAGGT.P

AGAAACGTCT

CAAGCATCGC

CATCATTAAA

TATC ITCAGT

AATCAGAACA

AAGCAATCAA

ATGCAGTTTT

CAATCGTAAG

TTGATAAAAA

TAGA-AACAA.A

CATTAGCATI

CAGCTGCTGA

CCAA-ACAAGC

CTTCATCAAC

CGCA.ACAAAC

GCTC LTCTTC

GTGGCCAAAC

CTGATCCAAC

TATGGGATGC

GCATTCCAAJ

GTAACTCAG9

GTCACCGTG.'

GCACAATCG'.

NITI'CATCA

'FITGGCGGAC

AGCAAAACAA

ATTTGATGAA

ATTACAACAA

AAATCAAGCA

AGATGCGGAC

TGCCAACAAC

AGCTGAAAAC

ACGTCAAGAT

AGAACAATCA

AGCAGAGCAA

TGCTGCAAA

AACAGAAGCP

AACTGAAACP

ATCAGAACAJ

TGGTGGTGG;

AATCAATGCI

AGGTTTGGCJ

TGATCACTG(

AATCATGGC(

TTGGGAAAT'

r AGGACACTCA

GTATTACTAT

AATGTTGATA

GGGGATTTAG;

AGCATGGC NT

GAAA'ITACAA

CGTGCATG=T

TCAG=TCAG

GACT'AATGC

GAGAAAAAAG

TTACTTTCTA

TCAGCTG3AAA

CACGCTTAG

*CCAGCTAAAG

CAAGCACCAG

ACTACACCAA

CCAGTACAAC

ACAGTTACAC

STTGAACGTC

k. GCTTCTGCAA

TCTCGTGGAC

3TGGTACA.ATC t' AACCCAGGT; k GCCTAA GTTCATTAAC C

AAAAAATTGA

cTrCACAAGT

TACGTGAACA

AC'ITGAATCA

AAGTTAATGG

ATGCAATCAG

AACAACAAAA

TGAAACAACT

AACAATCTGA

GTTCTAA-AGC

CTGCTGAACA

CTGAAGTGAA

CAAGCTCAAG

GTACAGATAA

CTACAACACC

CAACACCAGA

TACGTCAATC

CTGCTCGTGC

ATGAAGTTAT

AAACAAACAT

L TACGCGTGT

CTATCAGCA

k.GAAAAAAAT kTCTTCTTTA k.AAGCAAACA

A.CGTATTGAA

A~CAAAGCACA

CCGTGTTCAA

AGAAGACAAA

TGAAGCAACA

ATTAAACGTA

TGGCTTAGAA

AAAAGCTGCA

AGCAGAAGCA

CTCAGCAACT

TAGTGCAACA

AAG;CGATAAT

ACCAACACCA

ATTAGGTA

AGCACAAGTT

CGCAATTATG

CGTAACAGCT

CGG'ITTTGGT

EF129-2 (SEQ ID NO:482) 1 240 TABLE 1. Nucleotidc and Amino Acid Seqeuences of E.faecalis Genes.

VYK'KRLFASV LLCSLTLSA I ATPS IALACN VDKKIEECNQ LIS SLKAKQG DLASQVSSLE

AEVSSVFDES

MLDAVLDADS

AELETKRQDL

EKAKQAAAKP

NTGSSSSEQP

PVVWDAGLAA

GSGHROWEIN

MALREQKQTL

VADAISRVQA

LSKQSELNJM

AKAEVKAEAP

VQPTTPSDNG

SATARAAQVE

PG ITRVGFGY

KAKSEQLQQE

VSTIVSANND

KASLALEQSS

VASSSTTEAQ

NNGGQTGGGT

AGGIPNDHWS

SGSTIVGHSA

ITNL.NQRIEK

LMQQQKEDKQ

AESSKAGLEK

APASSSSATIE

VTPTPEPTPA

RGDEVIAIMW

RNEAIKNQAR

AVVDXKAENE

QKAAAEAEQA

SSTQQTTETT

PSADPTINAL

APGNSVIMAW

DVQVNGQSTT

KKVKQLE-ATE

RLAAEQKAAA

TPSTONSATE

NVLRQSLGLR

YNETNMVTAS

EF129-3 (SEQ ID NO:483) GGAC AATGTTGATA AAAAAATTGA ACAAAAAAT--

C

C

C.

CAAGAAAT

GAAGCAGAAG

CT-AAAkAGCAA

AAACGTAACG

ACAATGCTAG

GCTGTTTCAA

CAAGCCGTTG

GAAGCCAAT

ATGAAAGCTI'

AAACAAAAAG

GCTGAAAAAG

CCAGTTGCCT

GAATCAAGCA

GAAAATACTG

cGAAATAATG

GCGCCTTCTIG

CGTCCAGTAG

GA.AGCAGGTG

TGGGCGCCAG

TCAGGAAGCG

TACTCAGGTA

CATCATTAAA

TATCTTCAGT

AATCAGAACA

AAGCAATCAA

ATGCAGTTI'T

CAATCGTAAG

'FTGATAAAAA

TAGAAACAAA

CATTAGCATT

CAGCTGCTGA

CCAAACAAGC

CTTCATCAAC

CGCAACAAAC

GCTCTTCTTC

GTGGCCAAAC

CTGATCCAAC

TATGGGATGC

GCATTCCAAA

GTAACTCAGT

GTCACCGTGA

GCACAATCG7

AGCAAAACAA

AT?1'GATGAA

ATTACAACAA

AJAATCAAGCA

AGATGCGGAC

TGCCAACAAC

AGCTGAAAAC

ACGTcAAGAT

AGAACAATCA

AGCAGAGCAA

TGCTGCAAAA

AACAGAAGCA

AACTGAAACA

ATCAGAACAA

TGGTGGTGGA

AATCAATr-CA

AGGTTTGGCA

TGATCACTGG

AATCATGCCG

TTGGGAAATlI

AGGACACTC;

GGGGATTIrAG

AGCATGGCTT

GAAATT.ACAA

CGTGATGTTC

TCAGTTGCAG

GAC "A.ATGCC

GAGAAAAAAG

TTACTTTCTA

TCAGCCAAA

GCACGCTTAG

CCAGCTAAAG

CAAGCACCAG

ACTACACCAA

CCAGTACAAC

ACAGTTACAC

'TCGAACGTIC

*GCTrCTGCAA

TC'ICGTGGAG

TGGTACAATG

AACCCAGGTA

GCC

:TTCACAAGT

1'ACGTGAACA

ACTTGAATCA

AAGTTAATGG

ATGCAATCAG

AACAACAAAA

TGAAACAACT

AACAATCTGA

GTTCTAAAGC

CTGCTGAACA

CTGAAGTGAA

CAAGCTCAAG

GTACAGATAA

CTACAACACC

CAACACCAGA

TACGTCAATC

CTGCTCGTGC

ATGAAGTTAT

AAACAAACAT

TTACGCGTGT

ATCTTCTTTA

AAkAGCAAACA

ACGTATTGAA

ACAAAGCACA

CCGTGTTCAA

ACAAGACAA

TGAAGCAACA

ATTAAACGTA

TGCCTTAGAA

AAAAGCTGCA

AGCAGAAGCA

CTCAGCAACT

TAGTGCAACA

AAGCGATAAT

ACCAACACCA

ATI'AGGTTTA

AGcACAAGTT

CGCAAITATG

GGTAACAGCT

CGGTTTTGGT

EF129-4 (SEQ ID NO:484) ON VDKKIEEKNQ EISSLKAXQC- OLASQVSSLE

AEVSSVFDES

MLDAVLDADS

AELETKRQDL

EFKAKQAAAKP

NTGSSSSEQP

PV'JWDAGLAA

C SGH RDWE IN

MALREQKQTL

VADAISRVQA

LSKQSELNVM

A.KAEVKAEAP

VQPTTPSD4G

SATARAAQVE

PG ITRVGFGY

KAJCSEQLQQE

VSTIVSANND

KASLALEQSS

VASSSTTEAQ

NNGGQTGGGT

AGGIPNDHWS

SGSTIVGHSA

ITNLNQRIEK

LMQQQKEDKQ

AESSKAGLEK

APASSSSATE

VTPTPEPTPA

RCDEV IA 1MW

RNEAIXENQAR

AVVDKKAENE

QKAAAEAEQA

SSTQQTTE-TT

PSADPTINAL

APGNSVIMAW

DVQVNGQSTT

KKVKQLEATE

RLAAEQKAAA

TPSTDNSATE

NVLRQSLGLR

YNETNMVTIAS

EF130-1 (SEQ ID NO:485)

TGATACAITA

TTAATCAACA

CAGATACGTA

CCAGCTGATC

TCCTTGAGCA

GGTGCCATTG

GTCGCT'rTAT

AAAGGAGGGA

GT=ATTAGT

G'ITGGGTGGT

TGAAGAAAAA

CAGAAGCGGT

CGATACCAAG

TAACTGGTGC

AAATATGCGC

TTACTATTT

TCAACAAAAT

TATGGCTCGT

GATGAAAGCC

TGTCGAAATT

CGGGACCATG

CCAAA.AGAGA

ATCATTGGCT

AGCCCCTCGT

GAAACA.ACGT

CAATTTGAAA

AATTGCCCA

AAAGAAGATc

AAAAAAGACG

TAGCCTTAAT

ACGCCGTI'AG

TTGACTTTGA

ACAAAAACT

TTTTTAAACC

AAGTCATGGG

AAAAAATTGG

TTTTAACAAT

CAAGTTGAAA

IT~cAGTTGAG

ACCTGTGATT

ATTGTCcAAT

GAAAAACAAT

TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faeculis Genes.

TATGCCTTGG

ACCAAAAAAG

ACTTCTGTAG

AATATGATTA

GGAACATTIAG

CAATTGGAGC

CTAGTCATCG

ACGAACTCAT

AAAAAATCGA

CCTTAA ETAC

CAGCAACGAC

AAAAAACTTT

AACGAAGAT

TTATATCACT

ACCAACCCGT

CTGTGGCGAT

GCCTATTAAA

AGCCGATTGG

GGCGr'TTCCT

GATI'TATCTA

GTT1GAGTTAA

TTACAAGCAA

GACGCCAACG

GCGCTTAA

TATTTTCACC

CTGTTTATAC

TI'GATGACGT

CGACGCGA-AT

ACGATATGI'

TTTAGAAAGA

ATACAAAATA

TCCTCGTCAA

TGCTGTTCAA

GAAGGCTTTC

EF130-2 (SEQ 10 NO:486)

YIKRRENMRP

ADVKKNMARE

ALLTGAGTb4K

SVEKIEPTRV

LEQKTLADWV

KEKKRGKNWL

TTFDFDSVES

EDQVMGI<NNY

ELIDDVPGQN

INSLLVLLF I IGLAL IFNNQ LSTEAVMYAQ _yENKNLPVIG ALASHRTEDG VSLFSPLERT MITLITCGDL QATTRIAVQG I RSWVVQQNS

AIAIPSVEIN

KKDEL IY lTD TLAA PTP IKD

RSYAVSKLKP

LPIFKGLSNV

LSTVYTYKIT

ANDDMLKAFQ

EF130-3 (SEQ ID NO:487) CGITAG CAAGTTGAAA CCAGCTG-ATG TGAAGAAAAA TCCTTGAGCA CAGAAGCGGT GGTGCCATTG CGATACCAAG GTCGCTTTAT TAACTCGTGC TATGCCTTGG CTAGTCATCG ACCAAAAAAG ACGAACTCAT ACTTCTCTAG AAAAAATCGA A.ATATGATTA CCTTAATTAC GGAACATTAG CAGCAACGAC CAATTGGAGC AAAAAACTTT

TATGGCTCGT

GATGAAACC

TGTCGAAATT

CGGGACCATG

AACGGA-AGAT

TATATCACT

ACCAACCCGT

CTCTGCGAT

GCCTATTAAA

AGCCGATTGG

GAAACAACCT

CAATTTGAAA

AATDGCCCA

AAAGAAGATC

GGCGTTTCCT

GAITTATCTA

GTTGAGTTAA

ErACAAGCAA

GACGCCAACG

GTGGCT

TTGACTI'TGA

ACAAAAACTT

TTTTTAAAGG

AAGTCATGGG

TATTTTCACC

CTGTTTATAC

TGATGACGT

CGACGCGAAT

ACGATATGT'

TTCAGTTGAG

ACCTGTGATT1

ATTGTCCAAT

GAA.AAACAAT

TTTAGAAAGA

ATACAAAATA

TCCTGGTCAA

TGCTG'IICAA

GAAGGCTI'TC

EF130-4 (SEQ ID NO:488)

VSKLKP

ADVKKNMARE TTFDFD)SVES LSTEAVMKAQ FENKNLP'JIG AIAIPSVEIN LPIFKGLSNV ALLTGAGTMK EDQVMGKNNY ALASHRTEDG VSLFSPLERT KKDELIYITD LSTVYTYXIT SVEKIEPTRV ELIDDVPGQN MITLITCGDL QATTRIAVQG TLAATTPIKD ANDDMLKAFQ LEQKTLADTJTV A EF131-1 (SEQ ID NO:489)

TAGGCGGAGG

TGGCTTrTrTA

TTCTITTCA

CGCCGAAGTG

C ITGATCAAA

CAGGTTTTAG

CCAAACACAA

AAAGCAATTG

ATI'CAAGGTG

TTATCAGCAA

AATACCATTC

GCAGGAATGT

CACAAAACGG

AATTCAACA-A

TAACGCGTAT

TAGTATGTTTr

CTAGAGATTC

ATAATTATGC

AAATTCAAGA

TAAATAAAGG

GGTTCAGAT

AAGAAGGTAA

CTGAGGATAT

TGCCTAATAA

AAGTCAATAA

TAGAGAAAAT

CTGGTTAAA

GTTATAAATG

GCGTA-AACGA

GTTrGTGGTG

ACAAGTTAGT

GAATTTAACrG

AACAAATTAT

ATATGGCTTT

TGGCTCAATT

ACTTACATTA

TACGATTAGC

TATCGT1'ACC

AGGAAAATAC

GTATCAACGT

GAATTTTGGC

GACAGAAGAT

CATGCAAAGA

ATI'GGTGGTA

CAAGAATCGA

AAAGAAATAG

ATTGGTI'CGG

GCCAATTTTG

CAAAAATCTT

GATACAAAAC

GATATGTTGA

GATGAAGAAA

AATTATTCC C

ACCTATCAAG

TTCTATGAAA

AATCATATA

AAAGACATGG

GTGGTIATT

AAGTGGTC IT

TTGCACCAGA

CTTTGATCAT

AAAAGCAACA

TTACCACA-AC

TCGCTACGTI'

ATATGACAAG

TTATTCAATT

CACTAAATTT

AATTATTTAA

CCTTATTGGA

ACCAAGTGCT

AGGAGTGAAT

AATAAAAACG

GGAAGAAGAT

TAGTGGCGAA

TAAAGATGAT

AGCCAACACG

CTTGATCTTA

TTATCCGCAA

TGGTT1TAAAG

TGTTAAACAA

TGTCCTTTITA

TAATcTTTAT ACAGcccAAT

CTCAATTCCT

242 TABLE 1. Nucleotide and Amino Acid Seqeuences of E.faecalis Genes.

GCAGCTAGTT

TATTGGTACT

TTATGGACGT

TTACGT1T1AC

AAGACAGGGG

GGTTCGTTGT

TTGCCCATGA

TACATCAATT

CTTCTCAGCA

ACGGCGTTGA

TCATAT'TGCT

TCCATGATGT

ATTGGACT GGTAATGTGG AACGAGTC'GA CAFFTAGTTT AAGCTCTTAT CATGGCGGCA AGCGCGTCAA CAAGCCCTGG AACTAACTGT GTGAATCCAG AACCAATTTA ACTr.TTA.AAT

ATATGACGAC

CCACCGCACT

TCTATGTTCA

T TATT=IC

CGAAATACAA

TTIAA

AGGTGA=IG

TTTGCAAAAA

TGATAATTAT

AAAAGATATG

AGAATTAATT

EF131-2 (SEQ ID NO:490) MRKRH AK.KRHGGVNW LFIVCLLVVI GGSGYLIKTF FFTRDSQVSQ ESKWVLEEDR RSDNYANLTK EIVAPDSGEL DQKIQETNYI GSALIIKDDQ VLVNKGYGFA NFEKQQANTP NTRFQIGSIQ KSFTTTLILK AIEEGKLTLD TKLATFYPQI QGAEDIT'-SD MLNMTSGLKL SAMPNNIVTD EEIIQFVKQN TIQVNKGKYN YSPVNFVLLA GMLEIQ4YQRT YQELFNNLYH KTAGLKNFGF YETLLEQPNN STSYKWTEDN SYNQVLS IPA ASFAHEFGTG NVDMTTGDLY WYLHQLTSGH LVSTALLQKL WTSSQQSSYH GGIYVHDNYL RLHGVEAGQQ ALVLFSKDMK TGVILLTNCV NPAKYKELIG SLFHDVTNLT VKF EF131-3 (SEQ ID NO:491) a a TTT AATAAAAACG TTCTTTTTCA CTAGAGATTC ACAAGFI'AGT CGCCGAAGTG ATAATTATGC GAATTAACG CTTGATCAALA AAA~rCAAGA AACAAATTAT CAGGTTTTAG TAAATAAAGG ATATGGCT CCAAACACAA GGTCAGAT TGGCTCAATT AAAGCAATTG AAGAAGGTAA ACTTACATTA ATCA.AGGTG CTGAGGATAT TACGATTAGC TTATCAGCAA TGCCTAATAA TATCGTIACC AATACCATTC AAGTCAATAA AGGAAAATAC GCAGGAATGT TAGAGAAAAT GTATCAACGT CACAAAACGG CTGGTTTAAA GAAT I"TGGC AATTCAACAA GTTATAAATG GACAGAAGAT GCAGCTAGTT T'TGCCCATGA ATTTGGGACT TATTOCTACT TACATCAATT A.ACGAGTGGA TTATGGACGT CTTCTCACCA AAGCTCTTAT TTACGTTTAC ACGGCGTTGA AGCGGGTCAA AAGACAGGGG TCATATTGCT AACTAACTGT GGTTCGTTIGT TCCATGATGT AACCAATTTA

CAAGAATCGA

1AAGAAATAG

A.TTGGTTCGG

GCCAATTTG

CAAAAATCT

GATACAAAAC

GATATGTTGA

GATGAAGA.A

AATTATTCCC

ACCTATCAAG

'ITCTATGAAA

AA~rCATATA

GGTAATGTGG

CATTAGTI'

CATGGCGGCA

CAAGCCCTGG

GTGAATCCAG

ACTG~rAA-AT

AAGTGGTCTI'

ITCACCAGA

CTrTGATCAT

AAAAGCAACA

TTACCACAAC

TCGCTACGTT

ATATGACAAG

TTA'ITCAAT1'

CAGTAAATI'

AATI'ATTI'AA

CCT1'ATTGGA

ACCAAGTGCT

ATATGACGAC

CCACCGCACT

TCTATGTTCA

ITITATTTTIC

CGAAATACAA

TT

GGAAGAAGAT

TAGTCGCG;A

TAAAGATGAT

AGCCAACACG

CTTGATCTTA

TTATCCGCAA

TGGTTTAA.AG

TGTTAAACAA

TGTCCTTTTA

TAATCTF1'AT

ACAGCCCAAT

CTCAATTCCT

AGGTGATTG

T ITGCAAAAA

TGATAATI'AT

AAAAGATATG

AGAATTA.ATT

EF131-4 (SEQ ID NO:492) LIKTF FFTRDSQVSQ ESKVVtLEEDR RSDNYANLTK EIVAPDSGEL DQKIQETNYI NTRFQIGSIQ I(SFT'ITLILK AIEEGKLTLD SAMPNNIVTD EEIIQFVKQN TIQVNKGKYN KTAGLKNFGF YETLLEQPNN STSY1(WTEDN WYLHQL.TSGH LVSTALLQKL WTSSQQSSYH TGVILLTNCV NPAXYKELIG SLFHDVTNLT GSALI IKDDQ

TKIATFYPQI

YSPVNFVLLA

SYNQVLSIPA

GGIYVH]nNYL

VKF

VLVNKGYGFA

QGAEDITISD

GMLEKMYQRT

ASFAHEFGTG

RLI4GVEAGQQ

NFEKQQANTP

MLNMTSGLKL

YQELFNNLYH

NVDMTTGDLY

ALVLFSKDHK

EF132-1 (SEQ ID NO:493) TAGTTTCTAATCTCACcAAAAAAAATTTTA

ACAAAGAAGGAGAGATCGTATATAGAATGGATAT

rGTGGGAAGTCTGGGAATG TGATTGCTCTTrATATCGGGGCATGTTCAACApLATAGTAA GACAAGTC~r 243 TABLE 1. Nucleotide and Amino Acid Seqeuences of E. faecalis Genes.

GCTTCGAACGAAAAATTAAAGGTAGTAGTACTAATTCGATTI'AGCAGATATTACTGAAA-ATATAGCAAAAGATAAA

ATTGATTTACACAGTATCGTACCTATTGGGAAAGATC CCCACGAATATGAACC tTTGCCTGAAGATGTTCAAAAAACT TCAACACAGATTGATTTTTTATAACGGTGTAACTTGGAmACTGGAGGAAATGCTTGmACAAAATTAGTAAAA

MATGCGAACAAAGAGGAAAACAAGACTATTTTGCAGCAAGTGATGGCATAGATATTACTAGAGGGTCAGACT

GAGAAGGGAAGGAAGATCCCCATGCTTGG ITAAATTTAGAAMACGGTATTATTTACGCTAAAAATATTGAAAAATIG TTAGCGGAAAAAGATCCTATAATAAAAAACTATAAAGAAAATCTAGATAAGTATATTG

TGGATCTCTA

GACAAAGAAGCTAAATCTAAATTCTCAATTCCGAATGATAAA AAAATGATTGTTACAGTGAAGGATCtAAA

TATTTCTCGAAAGCGTATAATGTGCCTTCTGCTTACATTTGGGAAACAACACTGAAGAAGAAGGAACACCAGATCAA

ATAAATATAAATCCCAAACTCTCTTCTGAGATTGCAAAC

ATGAAAACAGTATCAAAAGATACCAATATCCTATCTATTCAAC ATTTACTGATTC GCAGA-AGACAA

GATGGTGATAGTTACTATGCGATGATGAAATGGAACCTGGATAAATTGCTGAAGGCC'I=CGAAATAA

EF132-2 (SEQ ID NO:494) MMRKWXVVVGSLGMLIALF I FCACSTNSKDKDTVASNEKLKVVVTNS I LAD ITENI AXDK I DLHS IVP IGKIDP HEYEP LPEDVQKTSKADL~I FYNGVNLXTGCNAWFTKLVKXANX<EENKDYFAAS DGIDVIYLEGQSEKGKEDPHAWLNLENG I I YAKNI EKWLAEKDP DNKKFYKENLDKYI EKLDSLDKEAKSKFAS I PNDKKMIV'rSEGCFKYFSKAYNVPSAY IWE INT EEEGTPDQ IKH.LVEKLRTTKVPSLFVESSVDDRPMKTVSKDTN t P IYST IFTDS IAEKGQDGDSYYAMMr'KWNLDKIAE

GLSK.

EF132-3 (SEQ ID NO:495) ATTCAATGAAGCAG*...GCTGACAAArA

GATATAT.ATGT

*AGCAGATATTACTGAPATATAGCAAAGATAAAATTGATTACACAGTATCGTACCTATGGAAGATCCCCACGA

*ATATGAACCtT CGAAGTAkAACMAACGTTATTTAACCGTATGAA

TGGAGGAAATGCTTGG=ITACAAATTAGTAAAAMATGCGAACAAAGAGGAAACAAAGACTAITTTGCAGCAAOTGA

TGCTGTTA ATAAGTCGGGGAGGAG.GTCCAGTGTAATAAA *CGGTATTATTTACGCTAAAAATATTGAAAAATTAGCGGAAAATCCTGATAATA

TTCTATGAA

AGAATGA.GTGATTTGCAAAGTATTAATGTC.ATCATAA

AAAATGATTGTTACAAGTGAAGGATGCTTtAAATA=TCTCGAAGCGTATAATGTGCCTTCGCTACATTGGGA AAtCAkACACTGAAGAAAAGGAACACCAGATCAAATAACACTTAGTTGAAAA

ACGCACCAGTCCCTC

.9CTATTCGTAGAAAGTAGTGTGGACGATAGACCATGAAACATATCAAAAGATACCATATCCTATCTATCAAC

AA'FTGCTGAAGGCCTTTCGAAA

EF132-4 (SEQ ID NO:496) .9C CSTNSKDKDTVASNEKLKVVVTNS ILADI TEN IAKDK I DLHS IVP IGKDPHEYEPLPEDVQKTSKADL I FYNGVNLXT CGNAWFTKLVKXANKEENTKDYFAASDG IDVIYLEGQSEKGKEDPHAWLNLENGI IYAKNIEKWLAEKDPDNXKFYKEN 9..LDKY I EIKLDSLDKEAKSKFAS I PNDKKMIVTSEGCFKYFSYAYNVP SAY IWEINTEEEGTPDQ IKHLVEKLRTTKVP S LFVESSVDDRPMKTVSKDTNI PIYSTIFTDS IAEKGQDGDSYYAM4MKWNLDKIAEGLSK :0 *0 .0.

Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

Query GenBank GenBank Gene Description BLAST BLAST Access. No. Score P-Value EF002-2 giJ2338759 (AF0 18073) periplasmic sorbi tol -binding protein; SmoE 113 3.6013-18 Rhodobacter EF003-2 giJ1552773 hypothetical (Escherichia coli] >gn]IPIld 10l12634 hypothetical 278 1.2013-53 29.4 EF003-2 giJ219 6 99 6 lipoprotein homolog [Treponema pallidum] >giJ2 108234 29K 309 3.30E-44 _______protein EF003-2 gi1146649 lipoprotein-28 precursor [Escherichia coli] >gi]2905 10 263 9.2013-40 EFOO3-2 giJ148838 28 3kDa membrane protein [Haemophilus influenzae] 197 2.10OE-39 EF003-2 gi11573 6 1 4 28 kDa membrane protein (hlpA) [Haemophilus influenzae] 197 7.80E-39 EF003-2 giJ23 14748 (A-E000654) outer membrane protein [Helicobacter pyloril 263 4.60E'-37 EF003-2 giJ349530 lipoprotein [Pasteurella haemnolytica] >gill 50508 lipoprotein 189 4.I1OE-29 EF003-2 gnlID~e 1l8435 similar to hypothetical proteins [Bacillus subtilis] 158 2.70E-26 EF003-2 giJ349532 lipoprotein [Pasteurella haemolytica] >pirIJNO753IjN0753 outer 200 1 .2013-25 EF003-2 gi11336657 lipoprotein [Bacillus subtilisl_ 182 2.70E-25 EF003-2 grilIPlDIe233873 hypothetical protein (Bacillus subtilis] >gnIjPlDjel 182900 186 1.3013-23 EF003-2 gi1294071 lipoprotein 3 [Pasteurella haemolytica] 199 76.60E-23 EF0O3-2. gi134953 1 lipoprotein [Pasteurella haernolytical >pirIJNO7521JN0752 outer 198 1.30E-20 EF003-2 gi129 4070 lipoprotein 2 [Pasteurella haemnolytica] 198 1.80E-20 EF005-2 giJ537235 Kenn Rudd identifies as gprmB [Escherichia colij >gill1790856 127 6.20E-12 EF006-2 gij 1552773 hypothetical [Escherichia coli] >gntIPlDjd 10 12634 hypothetical 255 1.40E-60 _29.4 S S 5.5 0 4 0 0 5 5 *50 0 S* 0 S S SO *5 S S Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF006-2 gi1349532 lipoprotein [Pasteurella haemolytica] >pirlJN07531JN0753 outer 221 6.40E-49 EF006-2 gi123 14748 (AE000654) outer membrane protein [1lelicobacter pylori] 283 2.70E-48 EF006-2 g112 196996 lipoprotein hornolog [Treponema pallidum] >giI2 108234 29K 267 4.40E-47 protein EF006-2 gnljPIDI 18435 similar to hypothetical proteins [Bacillus subtilis] 359 1.80E-44 EF006-2 giI3 4 9 53 1 lipoprotein [Pasteurella haemolytica] >pirlYN07521JN0752 outer 218 3.80E-41 EF006-2 gi1294071 lipoprotein 3 [Pasteurella haemolytica] 220 2.30E-38 EF006-2 gil 1466 4 9 lipoprotein-28 precursor [Escherichia coli] >giJ 2 905 10 193 2.60E-38 EF006-2 giJ294070 lipoprotein 2 [Pasteurella, haemolytica] 218 1 .20E-36 EF006-2 gil 14 883 8 28 kA membrane protein [Haemophilus influenzael 112 8.50E-34 EF006-2 gi1 1573 6 1 4 28 kDa membrane protein (hipA) [Haemophilus influenzae] 112 I.50E-33 EF006-2 gil3495 30 lipoprotein [Pasteurella haemolytica] >gill150508 lipoprotein 114 4.30E,-29 EF006-2 gi1294069 lipoprotein 1 [Pasteurella haemolytica] 114 1.30E-27 EFO6-2 gi13 6 7 lipoprotein [Bacillus subtilis] 202 2.10E-26 EF006-2 gnIPIDje233873 hypothetical protein [Bacillus subtilis] >gnhIPIDjeI 182900 200 6.50E-25 EF008-2 giJ49301 7 endocarditis specific antigen [Enterococcus faccalis] 1590 12.70E-21 I EF008-2 giJ 3 9 32 69 adhesion protein [Streptococcus pneurnoniae] 986 1.80E-129 EF008-2 gi1153 8 3 4 adhesin specific for salivary pellicle of dental surfaces 973 l.0013-127 EF008-2 giJI575030 surface adhesin A precursor [Streptococcus pneumoniae] 934 2.90E-126 EF008-2 gil 153826 adhesin B [Streptococcus sanguis] >pir1A435831A43583 adhiesin 916 3.90E- 126

BI

EF008-2 gi1 11849 32 IScbA [Streptococcus crista] 915 3.40E- 125 EF008-21 giL 117994 Isurface antigen A variant precursor [Streptococcus pneurnoniae] 1917 5.0-124 0 000 *00 @0 000 0 000 0 0 0 00 0 *00 000 00* 0 0 0 000*0e 0 0 0 *00000.

00*0000 00 0 0 0 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF008-2 giI3 10633 adhesin [Streptococcus gordonij 891 6.OOE- 122 EF008-2 gnljP1Dje255529 lipoprotein [Staphylococcus epidermnidis] 476 1.20E-99 EF008-2 gil 1573330 adhesin B precursor (fim.A) [Haemophilus influenzae] 380 1 .60E-68 EF008-2, 1i1245464 __YfeA [Yersinia pestis] >gi112 45 464 YfeA [Yersinia pestis] 355 1.20E-64 EF008-2 giJ755075 periplasmic-binding protein [Synechocystis sp.] 321 1 .70E-62 >gnlPIDId10 18652 EF008-2 gil 133 5 91 2 EwIA [Erysipelotlirix rhusiopathiae] 232 4.40E-42 EF008-2 gnlPID el 18595 similar to ABC transporter (membrane protein) [Bacillus 204 4.10E-38 EF008-2 g1177793 3 TroA [Treponema pallidum] 181 2.40E-35 EF009-2 giJ 34 9 53 1 iorti [Pasteurella haemolytical >pirl1N07521JN0752 outer 391 4.OOE-64 EF009-2 gi0 552773 hypothetical [Escherichia coli] >grilIP1DId 10 12634 hypothetical 359 1 .9013-63 129.4 EF009-2 gil29 4 0 7 0 lipoprotein 2 [Pasteurella haemolytica] 391 6.40E-63 EF009-2 gi3 4 95 32 lipoprotein [Pasteurella haenolytica] >pirlJN0753lJN0753 outer 386 1. 1OE-'61 EF009-2 gi11488 38 28 3kDa membrane protein [Haemophilus influenzae] 286 5.60E-60 EF009-2 gil] 5 7 36 1 4 28 kDa membrane protein (hIpA) [HaemopHius influenzae] 286 7.60E-60 EF009-2 gi2940 69 lipoprotein 1 [Pasteurella haernolytical 122 4.70E-59 EF009-2 gi 14 6649 lipoprotein-28 precursor [Eschericbia coli] >gi 12905 10 326 -2.20E-58 EF009-2 gi3495 3 0 lipoprotein IPasteurella haemnolytica] >giIl5 0508 lipoprotein 239 7.80E-57 EF009-2 giJ29 4 07 1 lipoprotein 3 [Pasteurella haemolytica] 344 4.90E-5_6] EF009-2 gi12 3 1 47 48 (AE000654) outer membrane protein [Helicobacter pylori] 319 4.2E-53 EF0O9-2; giJ21969 96 lipoprotein homolog [Treponema pallidum] >giJ2 108234 29K 312 2.60E-41 _protein S. *5* 9*

S

5

S

S A S S See...

S S 55 5. 5 5 5 S S S S S* S S *S S S .5 5 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF009-2 gi1 1336657 lipoprotein [Bacillus subtilis] 234 4.OOE-32 EF009-2 gnlIPIlfe233873 hypothetical protein [Bacillus subtilis] >gnljPlDI 182900 242 1.40E-31 EF009-2 gniljPIDIel 18435 similar to hypothetical proteins [Bacillus subtilis] 102 6.80E-22 EFOl 1-2 gnlIDIdIOO96 ferric anguibactin-binding protein precusor FatB of V. 579 3.10E-98 EFOI1I-2 gnlIDIdlOO96 ferric anguibactin-binding protein precusor Fat3 of V. 579 3. 1OE-98 EFOl 1-2 gnljPlDjeI 85374 ceuE gene product [Campylobacter colil 284-- 1 .30E-89 EFOl 11-2 guI 1PIDje 185374 ceuE gene product [Campylobacter ccli] 284 1.30E-89 EFOI 1-2 gi11507 56 40 kDa, protein [Plasmid piMI] >pirIA299281A29928 222 2.80E-52 membrane-associated EFO 11-2 gi115075 6 40 kDa protein [Plasmid pJMl] >pir1A299281A29928 222 2.8OE-712 membrane-associated_____ EF012-2 giJ309662 pheromone binding protein [Plasmid pCF 10] 266 8.70E-1 16 >tpir1B53309IB53309 EF012-2 gif3882 69 traC [Plasmid pADI) >pir1A533lI jA533 10 pheromone cADi1 252 1.IOE-109 binding EF012-2 gn~jPlDIdlOl 18 TRAC [Enterococcus faecalis] 281 3.60E- 103 EF012-2 gnlPlD~d1O65 TraC [Enterococcus faecalis] 277 2.30E-102 EFO 12-2 giJ3 12940 threonine kinase [Streptococcus equisimilis] 227 1 .90E-67 1531S28 153 EFOl12-2 giJ48 8 0 8 dciA-E [Bacillus subtilis] 228 1 170E-46 9SW 0@ 9 9 0 0 0 *0 SO 0* 0 0@ 0 a 0 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF012-2 pirIS 1665l1IS 1 6 6 dciAE protein Bacillus subtilis, 228 1.OOE-45 EFO 12-2 gnllPlDIel18149 (AJ002571) DppE [Bacillus subtilisi >gnllPl.Dlel 183316 228 3.80E-45 EF012-2 giJ400O5 OppA gene product [Bacillus subtilis] 281 3.90E-44 EFOI12-2 giJ143603 sporulation protein [Bacillus subtilis] >gnI PIDe e1183 163 281 7.70E-44 EFO 12-2 gn.1IPlDId 0156 Periplasmic oligopeptide-binding protein precursor. 152 2.20E-43 3 EFO 12-2 gi1157 4 679 oligopeptide binding protein (oppA) [Haemophilus influenzae] 178 2.20E-42 EFO 12-2 giJ47802 Opp A (AAI1-542) [Salmonella typhim-urium] >giJ4780 8 128 1.OOE-37 -precursor_ EFOI12-2 giJ8 82 SSO ORF -f535 [Escherichia cold] >gi11789 397 (AE000384) f535; 228 5.30E-36 This 535 EF014-2 pirID7O070ID70 transcriptional regulator homolog ywtF Bacillus subtilis 101 1.40E-J7 0__ EFOI14-2 gnjPlDe 11698 8 capsular polysaccharide synthesis protein [Streptococcus 121 9.5.0E-27 EFO 14-2 giJ280 4 7 6 9 (AF030373) putative regulatory protein [Streptococcus 121 9.50E-27 pneumofliae] EF014-2 gn1lPIDfe2891 2 6 unknown [Streptococcus pneumoniael 121 1.OOE-24 EFOI14-2 giJ22 6 7 23 9 ORFi [Staphylococcus epiderrnidis] 234 .1.50E-24 EFOI14-2 gi14852 7 5 putative regulatory protein [Streptococcus pneunoiiiae] 121 3.90E-24 EF014-2 giJ280 4 7 35 (AF030367) putative regulatory protein [Streptococcus 121 3.90E-24 pneumoniae]____ EF014-21 giJ2 8047 47 (AF030369) putative regulatory protein [Streptococcus 121 3.9013-24 9 @000 *9* 0S 0@ 0@0 0 C@t 0 0 S *0 S 0 6 0 00 0 0 @0 9 @6 0 000 'to 0 .6 0*0 0*0 *0000 0 0 0 0 0 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EFO 14-2 giJ 1762327 putative transcriptional regulator [Bacillus subtilis] 1 85 2.80E-22 EF0 14-2 gi143 156 membrane bound protein [Bacillus subtilis] >gnlIPlDjI 184471 116 1.l1OE-21 EF014-2 gdIjP1D~dI189 membrane bound protein LytR [Synechocystis sp.] 113 6.20E-20 EF0I4-2 giJ12768 74 EpsA [Streptococcus thermophilus] 103 4.OOE- 17 EF0 16-2 gnlIlDjel 18566 similar to amino acid ABC transporter (binding protein) 194 3.70E-35_ EFO 16-2 gi1409 3 4 arginine binding protein [Escherichia colil >gi176979 4 arti 121 1 .60E-31I EF016-2 gn]IP1D~dI152 Arginine-binding periplasmic protein 2 precursor [Escherichia 121 4.80E-31 7 EFOI16-2 giJ687 65 2 FINY [Escherichia coli] >gn1lPIDIdt 016464 FINY protein 160 5.70E-31 precursor. EFOI16-2 giJ26504lJ 0 (AE001 090) glutamine ABC transporter, periplasmic glutamine- 122 3.30E-29 _binding EFO 16-2 giJ16 4 9 03 5 high-affinity periplasmic glutam-ine binding protein [Salmonella 104 1.80E7.27 EF016-2 gil 1574634 ,glutamine-binding periplasmic potein (glnH) [Haemnophilus 174 2.50E-27 EF016-2 giI4 1569 GInH precursor (AA -22 to 226) [Escherichia coli] 106 4.70E-27 ~>gnlPIDId1 015250 EF016-2 gn]IP1DjdI0 152 Arginine-binding periplasmic protein I precursor [Escherichia 109 .3.70E-26 EFO 16-2 giJ7697 9 1 artI [Escherichia coli) >gi7 69 79 l arti [Esclerichia coli] 127 2.30E-25 EF016-2 gnljPlDjdlOO89 homologous to Gin-binding periplasmic proteins [Bacillus 117 8.50E-24 2 EFO16-21 gi115 4 12 5 J protein [Salmonella typhimurium] >gi4771 8 reading frame 118 21IOE-23 *b

C

C

C

Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

hisJ EFOI6-2 gnIjP1DdlI168 HISTID[NE-BINDING PERIPLASMIC PROTEIN 117 4.50E-23 8 PRECURSOR EFOI16-2 gill 166636 histidine-binding periplasmic protein HisJ [Escherichia cclii 117 6.60E-23 EF017-2 giJ 3 8 826 9 traC [Plasmid pAD I) >pirlA533l I A533 10 pheromone cADi1 421 4.50E-128 binding EF017-2 gnljPIDjdl0l 18 TRAC [Enterococcufs faecalis] 417 5. 113E-124 EF017-2 gn1jPlDjdl0065 TraC [Enterococcus faecalis] 414 4.4013-123 EFOL17-2 giJ3O96 62 pheromone binding protein [Plasmid pCF 101 415 2.40E-l 119 __________>pirIB53309IB53309 EFO17-2 giJ4 05 OppA gene product [Bacillus subtilis] 294 -6.20EA82 EFO 17-2 giJ143603 sporulation protein [Bacillus subtifis] >gn1jPIDjel 1183163 290 2.80E-79 EF0 17-2 giJ3 12940 threonine kinase [Streptococcus equisimilis] 241 2.40E-71 ~>pirlS28 1531528 153____ EF017-2 ___t4880 8 dciAE [BaciULus subtilis] 270 1. 1OE-61 EFO17-2 gnlPIDjell8I49 (AJ002571) DppE [Bacillus subtilisi >gnljPlDI 183316 270 1.5OE-61 EFO 17-2 pirlS 16651 IS]66 dciAE protein Bacillus subtilis 270 3.I1OE-60 EF017-2 giJ30492 5 periplasmic oligopeptide binding protein [Echerichia coli] 171 2.60E-57 EF017-2 gil 14701 4 oligopeptide binding protein precursor [Escherichia ccli] 171 8.70E-56 EFO 17-2 giJ478 02 Opp A (AAI-542) [Salmonella typhimunium] >giJ47 8 0 8 154 1.30E-52 p p.

p p p p .1 .1 0. 0 .*p Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

F0 17-2 giJ88255O ORF-f535 [Escherichia coli] >gi11 78 93 97 (AE000384) [535; 135 5.50E-52 This 535 aa EFO 17-2 giJ157 4 67 9 oligopeptide binding protein (oppA) [Haemopliilus influenzae] 168 2.90E-43 EFO 19-21 gi1438 45 8 lRely N-terminal signal sequence; mature protein probably 104 2.30E- 17 EF02 1-2 grnlIPID1e611492 unknown [Bacillus subtilis] >gnljPDIel 184232 similar to ABC 317 2.50E-103 EF02 1-2 bbsj 173803 CD4+ T cell-stimulating antigen [Listeria monocytogenes, 476 2.80E-81 8 5 EQ- 1167, EF021-2 giJ58lSO 9 tmbC gene product [Treponema pallidum] >pir1A435951A4359 5 152 3.20E-71 membrane EF02 1-2 giJ26 88280 (AE00 1143) basic membrane protein C (bmpC) [Borrelia 101 5.50E-27 burgdorferi] EF02 1-2 gnl1PlDIel 17283 membrane protein A [Borrelia garinii] 142 6.50E- 2 EF02 1-2 gnilPIDfe 117283 membrane rotein A [Borrelia burgdorferi] 141 -9.20E-'22 EF02 1-2 gnIjPIDje 117283 membrane protein A [Borrelia burgdorferi] >giI5 16592 141 9.20E-22 ,membrane EF021-2 EF021-2 EF021-2 EF02 1-2 gril PDle 117283 gi150 84 21 7-gI-17-53 22 5 gnljPDjel 1172R82 gn1jPIDje 117283 ~gnIPIDI 17283 IgnjjP1Djel 178 bmpA(p39,ORFI) [Borrelia burgdorferil antigen P39 [Borrelia burgdorferi] >gil2 68 82 8 l (AEOO1 1143) I It 1.70E-21 basic____ BmpA protein Borrelia burgdorferij- 141 2.70E-20 membrane protein ALBorrelia afehl 141 8.60E-20_ membrane protein A [Borrelia aftelfii 141 8.60E-20 membrane protein A [Borrelia afzelii] 141 8.60E-20 bmp~p9,RF Borrelia burgdorferi] 141 1 .50E- 19 9e*

S

S

S

S

*.S

S S S S *S 55 5 S S

S

S Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF022-2 giJ3 12940 thieonine kinase [Streptococcus equisimilis] 324 5.9013-66 1531528153 EF022-2 giJ 30 9 662 pheromone binding protein [Plasmid pCF 10] 307 5.60E-60 ___________>pirIB53309IB53309 EF022-2 gnljP1Ddl0l 18 TRAC [Enterococcus faecalis] 301 4.80E-59 EF022-2 gnIPIDI 18149 (AJ002571) DppE [Bacillus subtilis] >gnljPIDI 183316 170 5.10E-59 EF022-2 giJ4 880 8 dciAE [Bacillus subtilis] 170 5.20E-59 EF022-2 gnlIDIdlO065 TraC [Enterococcus faecalis] 299 2.80E-58 EF022-2 pirIS 16651 IS 1 66 dciAE protein Bacillus subfilis 170 1.60E-57 EF022-2 gil38 82 6 9 traC [Plasrnid pAD I >pir1A533 I01A533 10 pheromone cADi1 290 2.70E 53 S~~binding EF022-2 giI40005 OppA gene product [Bacillus subtilis] 154 7.30E-48 EF022-2 gi11436 03 sporulation protein [Bacillus subtilis] >ghlIPIDjel 1183163 154 3.I1OE-47 EF022-2 giJ268 8 2 2 7 (AEOO 1139) oligopeptide ABC transporter, periplasmic 215 1.OOE-36 EF022-2 giJ228l 4

S

8 (AF000366) oligopeptide permease homotog All IiBorrelia 215 l.0013-36 burgdorferil_____ EF022-2 giJ30 4 925 Periplasmic oligopeptide binding protein Escherichia coli] 1 31 1 .30E-35 EF022-2 gil 14701 4 oligopeptide binding protein precursor [Escherichia coli] 131 1.80E-34 EF022-2 giJ47 802 Opp A (AAI-542) [Salmonella typhimurium] >gi147 80 8 138 4.90E-34 EF023-21 gil 30 9 66 2 lpheromone binding protein [Plasmid pCF 10] 231 14.70E-66_ *9 e *4* 4 0 4 4. *4 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in Gen~ank and Derwent databases.

__________>piflB53309IB53309 EF023-2 giJ38 826 9 traC [Plasmid pADI]I >pirIAS33lIOIA533 10 pheromone cADI1 223 4.80E-62 binding EF023-2 gnljP1Djd1O1 18 TRAC [Enterococcus faecalis) 226 1.OOE-58 EF023-2 gnlPDId1065 TraC (Enterococcus faecalis] 226 4.40E-58 EF023-2 giJ4 880 8 dciAE [Bacillus subtilis] 157 1.20E3-7 EF023-2 gnlIPlDje1I8l49 (Ai002571) DppE [Bacillus subtilis] >gnljPIDjel 183316 157 1.20E-57 EF023-2 pir166511 6 6 dciAE protein Bacillus subtilis, 157 3.80E-56_ EF023-2 giI40 0 05 OppA gene product [Bacillus subtilis] 137 2.3013-53 EF023-2 gi11436 03 sporulation protein (Bacillus subtilis] >gnlPIDIel 183163 133 6.90E-43 EF023-2 giJ 4 780 2 Opp A (AAI1-542) [Salmonella typhimuriumi >gil47 8 08 135 2.OOE-4) precursor_____ EF023-2 giJ26882 27 (AEQO 1139) oligopeptide ABC transporter, periplasmic 187 9.4013-41 EF023-2 giJ228l 4 58 (AF000366) oligopeptide permease homolog All [Borrelia 187 1.90E-40 ~~burgdorferil EF023-2 gif 8825 50 ORFf535 [Escherichia coli] >gi117 89 39 7 (AE000384) f535; 155 l-30E-38 IThis 535 EF02-3-2 gi13049 25 1,eriplasrnic oligopeptide binding protein [Escherichia coli] 130 9.QOE-37 EF023-2 gi114701 4 oligopeptide binding protein recursor [Escherichia colil 130 170E-34 EF026-21 2352482 (AF005097) unknown [Lactococcus lactis] 141 1.I1OE-23 F027- 3062 heromone binding protein [Plasmid pCFI 109 8 6.20E-71 *6* 9* 9 9 9

I

9 9* 9 *9 9 9 9** 9 .9 9* Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

__________>pirlB53309IB5 3309 EF027-2 gnlPDIdI0065 TraC [Enterococcus faecalis] 202 1.50E-68 EF027-2 gnlJ1PIDIdl10 118 TRAC [Enterococcus faecalis] 202 1.50E-68 EF027-2 giJ38 8269 traC [Plasmid pAD I1] >pirIA533 I OA533 10 pheromone cADi1 213 8.30E-68 binding EF027-2 gnlPIDIe 181l 49 (AJ00257 1) DppE [Bacillus subtilis] >gnljIlDlel 183316 222 3.70E-41 EF027-2 gi 1 4 88 08 dciAE [Bacillus subtilisl 222 4.90E-41 EF027-2 pirlS 1665 1 S 166 dciABE protein Bacillus subtilis 222 LIlOE-39 EF027-2 giJ4 00 0 5 OppA gene product [Bacillus subtilis] 251 4.10E-39 EF027-2. gi11 436 0 3 sporulation protein [Bacillus subtilisi ]Dle 183163 247 5.80E-S9 EF027-2 EF027-2 EF027-2 gi13 12940 gi2688 227 gi1228 1458 threonine kinase [Streptococcus equtsimii'sj >pirIS28153IS28l53 (AEOOI 139) oigopeptide ABC transporter, peiplasmic 131 2.40E-24 (AF000366) oligopeptide permease homolog All [Borrelia 131 2.40E-24 EF027-21 giJ 22 S 1468 burgdorferi].

(AF000948) OppAIlV [Borrelia burgdorferi] >gil2 6 89 89

I

(AE000792) oligopeptide binding protein oppA) Haemophilus influenzae] B. subtilis alkaline phosphatase iIA; P 19405 secretory 117 13:O0E-20 EF027-21 EF028-2 gil 1574679 gnlIPIDjI 1204 130 996 I982 3 .50E-20 3.60E-131I 2.90E- 129

I

pirlB3W 6n3Ilklfe phosphatase (EC 3.1.3. 1) 111 precursor Bacillus 9** 9 *0e 9* 9.

9 9 9 9 99 9 9 9 9 9 9. C 3* .9 9 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF028-2 EF029-2 EF028-2 EF028-2 EF028-2 EF028-2 EF028-2 EF028-2 EF028-2 EF028-2 EF028-2 EF028-2 0 gi147 0383 giJ 143324 gil 147243 gil 147237 gill 47 23 9 gil 147241 gill1 27 7 127 gi1147 229 gi181 8851 gil 147245 gi1147231 g147235 gijl0l 6 OlO alkaline phosphatase A [Bacillus subtilis] >gnljPIDlel 182942 APase I [Bacillus lichenifonuis] >pir1A44828lA4482 8 alkaline alkaline phosphatase precursor (EC 3.1.3. 1) [Escherichia coli] alkaline phosphatase precursor (EC 3.1.3. 1) Escherichia colij alkaline phiosphiatase precursor (EC 3.1.3. 1) f[Escherichia coli) alkaline phosphatase precursor (EC 3.1.3. 1) [Escherichia coli] phoA gene product [Cloning vector pFWjol]>i1 7 I0 phoA gene a nelkaline hosphatase precursor (EC 3.1.3. 1) [Escherichia coli] alkaline phosphatase [synthetic construct] alkaline phosphatase (phoA) (EC 3.1.3. 1) [Escherichia fergusonii] alkaline phosphatase precursor (EC 3.1.3. 1) [Escheichia coli] alkaline phosphatase precursor (EC 3.1.3. 1) [Escherichia colij alkaline phosphatase with N-terminal PeIB-leader and Cterminal YncB [Bacillus subtilisi >gnillPlDlel 183421 similar to mnicrococcal simiar o hyothtica prteins rBacillus subtilis] nuclease [Staphylococcus aureus] >pirlA00790NCSAF mircoccal heonclease [Staphylococcus hicus] 803 184 183 178 178 178 174 178 174 177 174 174 174 4.80E-1 19 3 .00E-54 8.3013-54 4.40E-53 4.40E-53 4.40E-53 4.9013-53 8.4013-53 1. 1OE-52 1.20E-5,2 1 .6013752 1 .60E-52 1 .60E-52 257 V3:50E--551 EF029-2 Igif 1750126 EF29- EF029-2 Ra[PlDle 18360 giJ673492 263 320 7.80E-53 2.20E-39 15 5 1 9. IOE 39] e Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF029-2 g1147 146 thermonuclease [Staphylococcus intermedius] 145 4.90E-32 >vidS26079IS26079 EF030-2 *i48808 dciAE [Bacillus subtilisl 149 i. 1OE-66 EfO 3 0-2 lP1IDe11l8l49 AJ00257l)D E Bacillus subtilis >nlIP1De118331 6 149 1.50E-66 EF030-2 S16651 S166 dciA.E protein Bacillus subtilis 149 5.90E-66 EFO30-2 giJ3O966 2 pheromone binding protein [Plasmid pCF 101 227 7.40E-52 I>pir 853309 853309 nVII- rnlPIDW1dl1l 18 TRAC [EnterococCUS faecalis] 2377 7.0-2 9* 4* S S S S

S..

S S S S S S S. S S S S S S 55 S S S S S *5

S

Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF030-2 EF033-2 EFO3 3-2 EF033-2 EF03 6-2 EF036-2 [EF036..2 gil 1574679 gnlIDleI 18439 pirIS5443 7

IS

544 gil 1619623 gnllPIDldl 0102 2 gi126 2 2858 giJ2 622859 EF036.21 gi126 88 I 15 oligopeptide binding protein (oppA) [Haemophi lus influenzae] similar to iron-binding protein [Bacillus subtilis] hemin binding protein Yersinia enterocolitica hemnin binding protein [Yersinia enterocolitica] ORLF 108 [Bacillus subtilis) >gnljPIDlel 185766 alternate gene (AE000929) phosphate-binding protein PstS rMethanobacten'um (A.E000929) phosphate-binding protein PsLS homolog I'Methanobacteriufli (AEQO 132) phosphate ABC transporter, periplasmic phosphate-binding (AF05097) unknown [Lactococcus lactis] hypothetical protein [Escherichia colil >gil78 65 1 1 (AEOOO- 139) 2-5A-dependent RNase [Mus musculus] >pirIB45 771 lB4577 1 G9a [Homo sapiens] >pir1S30385IS30385 G9a protein human erythroid ankyrin Mus musculus] >pir.S37771 lS3 77 7 1 ankyrin, ankyrin [Mus musculus] >pil495 0 211 4 9 50 2 ankyrin mouse alt. ankyrin (variant 2.2) [Homo sapiens] ankyrin MHow saies ankyrin [Homo sapiens]__ ankyrin 1, erythrocyte splice form 2 human 148 164 108 108 544 183 1.40E-45 158 2.40E-41 1-I17 1 1.IOE-12 2.60E- 14 1.40E-l II 2.OOE-1 I1 1.20E-96 EF03 7-2 EF040-2 EF040-2 EF040-2 EF040-2 EF040-2 EF040-2 EF040-2 EF040-2 EF040-2 1i123 524 82 gil 16575 16 gi129 326 5 gi1287 86 5 gil3 11817 gill 191940 g17 8646 gil11845265 pirIA3 50491A3 5 0LL 141 208 105 143 119 119 120_ 120 120 120 1.10t23_ 1 .90E-29 1.OOE- 17 8.3 0E- 14 4.80E-13 4-90E- 13 1.50E-12 1.80E-12 1.80E-12 1.80E-12 i q U* S i .9 9 0 *9 S *9 S 0 9 0* 9* 9 9q 9 0 *9 to 9*9 9 9 5 4. S 4 Table 2. Closest matching sequen ces between the polypeptides of the present invention and sequences in GenBank and Derwent, databases.

C

C., C

C

C. C OC9 C C C C

C

C C C C C C eq

C

C

C..

.0 .00 .0 'a 0 0 C C 0 a C a 0 q. 0 C Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF041-2 gil 1 4 7 0 11 4 oligopeptide binding protein precursor [Escherichia coli] 160 1.50E-28 EF041-2 gi12253 286 (AF005657) plasminogen binding protein [Borrelia burgdorferil 134 5.OOE-27 EF045-2 giJ30885 4 oligopeptide binding protein [Lactococcus lactis] 437 3.2013-125 EF045-2 gi)49 5 1 8 1 oligopeptide binding protein [Lactococcus lactis] 426 9.70E- 124 EF045-2 gil6779 45 AppA [Bacillus subtilis] >gnljP1Dlel 183158 oligopeptide ABC 154 2.3013-31.

EF045-2 giJ29301 4 peptide-binding protein [Lactococcus lactis] 158 2.40E-14 >pirlB47098lB34709 8 EF048-2 ,gi1 1574060_ hypothetical [Haemophilus influenzae] >pir1164 1641164164 250 2 .30E-41I EF048-2 dbjjjA.B001488_. (ABOO 1488) SIMILAR TO C4-DICARBOXYLATE- 208 3.6013-34 2 BINDING PERIPLASMIC EF048-2 giJ466717 No defintifon. line found [Escherichia coli] >gill1 7 90 004 199 1.3013-30 EF048-2 gi1460O 6 periplasmic G4-dicarboxylate binding-protein [Rhodobacter 162 1.405'-25 capsulatus] EF048-2 gil157 3 10 2 hypothetical [Haemophilus influenzael >piflH641431H64143 244 3.8013-25 EF048-2 gi12182 530 (AE000085) Y4miM [Rhizobium sp. NGR,234] 114 _5.60E- 18 EF048-2 gi11 5 7 2999 hypothetical [Haemophilus influenzae] >pirE64141 JE64141 116 5-.90E-I15 EF049-2 gi11495 81 maturation protein [Lactobacillus paracasei] 241 2.40E-55 __________>pirIA448581A44858 EF049-2 giJ47 198 ORF (AA I to 299) [Lactococcus lactis cremoris] 239 1.OOE-54 ___________>pirlS080831SO 8 0 8 3 EF049-21 gI14324 02 maturation protein [Lactococcus lactis] >0i162305S5 proteinase 239 6.20E-54

S

0* 9.

S

VO .0 00 6 0 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in Gen.Bank and Derwent databases.

EF049-2 gi147283 5 ORFI [Lactococcus lactis cremoris] 241 1.5013-53 EF049-2 gi(39782 33kDa lipoprotein [Bacillus subtilis] >gnlPIle325 181 33kDa 128 8.90E-40 EF051-2 gnilPIDIdIOl 14 molybdate-binding periplasmic protein [Synechocystis sp.) 173 3.2013-50 2_ EFOS 1-2 gnlIP[DIe 118602 alternate gene name: yvsD; similar to rnolybdate-binding 314 5.9013-50 EF051-2 giJl574S 4 6 lsg locus hypothetical [Haemophilus influenzae] 161 2.20E-43 ~~>pirIA64 1751A64 175 EF051-2 giJ50449 8 periplasmic inolybdate-binding protein [Escherichia coli] 148 1.40EB-30 147817 EF051-2 gij14 8 9 39 ORE 8 [Haemophilus influenzae] >pirIS27583IS27583 150 8.IO13-28 hypothetical_____ EF054-2 giI150556 surface protein [Plasmid pCF 101 >pirA418261A4]826 probable 1490 1.8013-192.

EF054-2 gnlPiDle23657 1 cell wall anchoring signal [Enterococcus faecalis] 515 8.l1OE264 EF054-2 giJ457 3 8 ORFC [Enterococcus faecalis] >pirIJH0204IJHO2O4 372 1 .60E-58 ___________hypothetical 30.5K EF054-2 giJ4965 2 O orf iota [Streptococcus pyogenes] >pirIS68l 2 5IS 4 5O 9 l 362 1.30E-43 hypothetical_____ EF054-2 gill1 6 06 93 sporozoite surface protein [Plasmodium yoelii] 286 4.30E-33 >pir1A45559lA4555 9 EF054-2 gill 8135 2 3 PbTRAP [Plasmodium berghei] 305 1.3013-32 EF054-2 -gnIl PIDIe225 687 1zinc finger protein [Mus musculus] >gnlP1De225688 zinc 246 3.60E-26 A 'I4 I AnY7 ir EF054-2 giJ22903 94 lgG and IgE immnunoreactive antigen recognizea oy sera from patients 0*0 0 000000 0eS 0* 0 *09 0 0 0 0 0 0 0* 4 0 00 0 0 00*090 0 0 0 0 0 0 S. 0 0 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF054-2 EF054-2 EF054-2 EF054-2 EF054- I EF054-2 giJ 229 0 392 giJ 46523 pinIS 153301IFCS 0 g1620100 _gj163 686 1 gi163 689 lgG and IgE immunoreactive antigen recognized by sera from B antigen [Streptococcus agalactiael IgA Fc receptor precursor Streptococcus agalactiae Pro- and Glu-rich, PENPEV (10x); similar to Streptococcus

B

NF-M c-teriu Glugaus N F-M protein [Gallus gallus) >pirlS I 57621S 15762 neurofilament tri let TATA-box like sequence (Us 11) [Human herpesvinius

I)

>gil29I 4 93 18 cell wall anchoring sigal [Enterococcus faecalis] surface protein Plasmid pCFl10 >pin A4 1826 A4 1826 probable ORFC [Enterococcus faecalis] >pirIJHO2O4IJHO20 4 hypothetical 30.5K orf iota [Streptococcus pyogenes] >pirIS68 1251S45091 hypothetical sporzit suface protein [Plasmodium yoeli) 232 228 222 222 2.80E-23 L OOE-22 3I OE-21 6.90E-2 1 1 .5017-21 194j 4.1IOE- 19 EF054-2 1 g07 57867 EF059-2 EF059-2 9-2 gnljPlDe2 67 gil 15 0 5 56 giI457 3 8 418 606 366 5 60E-95 3 .70E,-87 9.30E-50 EF059-2 giJ49 6520 9-2 1n1 6 0 693 367 5.90E-44 344 =11013-38 EF059-2 EF059-2 EF059-2 >pirIA455591A4555 9 1iI813523 PbTRAP [Plasmodiunm ben heij e ysr rr gi12 2 9039 4 lgG and IgE immunoreactive antigen recognizedb eafo iatientIs giJ2 290 39 2 IgG and IgE irnnunoreactive antigen recognized by sera from Datients 295 251 251 3.0013-29 3 .4013-29 0 *0 0* .00 0. 00 .0 00 0 0 0 0 0* Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF059-2 gij 1620100 ]Pro- and Glu-rich, PENPEV (l Ox); similar to Streptococcus B 253 6.40E-27 EF059-2 giJ465 2 1 Fc receptor [Streptococcus agalactiae] >pirjA60234jA60234 IgA 197 2.70E-26 Fe EFOS9-2, giJ4 6 523 13 antigen [Streptococcus agalactiae]__ 232 9.30E-26 1 0-InT SEF059-2 1pirIS 1 53301IFCS IgA Fc receptor precursor Streptococcus agaiactiae EF059-2 EF059-2 EF059-21 EF061-2 1 EF061-2] EF061-2 EFO61-2 EF061-2 EF061-2 EF061-2 EF061-2 0 gil425356 giJ4577 69 gnlPIDje23657 1 gi11 50556 giJ49 6 5 2 0 giJ4573 8 gil813523 gill 60693 giJ6 3 6 8 9 zicfinger protein [Mus musculus] >gnljP1Dje225688 zinc zona pellucida protein [Pseudopleuronectes americanus] Collagen [Bombyx mnon] >pir1S42886IS42886 collagen silkworm cell wall anchoring signal [Enterococcus faecalis] surface protein [Plasmid pCF 10] >pirlA418261A4l826 probable oil' iota [Streptococcus pyogenes] >pirIS681I251S4S09

I

hypothetical OR.FC [Enterococcuts faecalis] >piriJHO2O4IJHO2O4 hypothetical 30.5K PbTRAP [Plasmodium. berghei) sporozoite surface protein [Plasmodium yoeliil 591A4555 9 NF-M c-terminus [Gallus gallus] ,NF-M protein [Gallus gallus] >pirS I 57621S 15762 neurofilainent triplet IlgG and IgE immnunoreactive antigen recognized by sera from 229 209 925 350 308 I- t 234 IA.4E-22 I OOE-21 7.60E- 19 8. IOE-I 18 1.50E-,07 IA.4E-58 322 1 6.40E-50 263 241 232 L OOE-26 -9:.O0E-25 2. 1OE-22 2.60E-22 EF06 1-2 Ig1229 0392 176 jT2.40E-21_ *9* 9** .9 .9 9 999 9 9 9 9 9 9 0 9 9.

9 9 9 9 9 9** 99* *99 9 9 9 9e 9 0@ .9999 9.999 99 .9 9 9 9 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

________patients EF061-2 gil 162 0 100 Pro- and Glu-rich, PENPEV (IlOx); similar to Streptococcus B 165 2.70E-20 EF061-2 gn]IPIDje225687 zinc finger protein [Mus musculus] >gnljPlDje225688 zinc 197 7.80E- 19 EFO6I-2 gi 1601355 interspersed repeat antigen [Plasmodium falciparuml 199 8.20E-l18 EF061-2 gi1410750 interspersed repeat antigen [Plasmodium falciparum] 199 8.90E- 18 EF061-2 giJ229O 388 lIgG and IgE immunoreactive antigen recognized by sera from 182 1.40E- 17 ton 1~17 EF061-2 EF062-2 EF062-2 EF062-2 EF062-21 E F062-2 EF062-2 gi[2 29 0 394 IgG and IgE iinmunoreactive antigen recognizea by sera fromn patients asal gene product (AA 1-1296) [Enterococcus faecalis] giJ470 4 9 gi143324 giI2 109266 gill15 05 55 gill 1009 73 giJ47 248 3716 0 aggegaionsubstance (ASPI1) [Enterococcus faecalis] ragregtio sustace Entrococcus faeciwn] aggregation substance [Plasmid pCF 101 >pirIH416621H4 1662 5523 0 6338 0 SspB precursor [Streptococcus gordonii] PAc protein precursor (AA -38 to 1527) [Streptococcus mutans] ~surface protein antigen precursor [Streptococcus sobrinus) YJUVI-31Y -t S 1.70E-38 -1 5iJ0L-3~ EF062-2 IgnIlPIDld 10 150 5-.00E-36 EF062-2 EF062-2 7 giJ4726 7 bbs1 148453 cell surface antigen 1111 [Streptococcus mutans] >PiTlS068391S06839 t 1 6.5013-36 1.2 E-3 SpaAendocarditis imnmunodominant antigen [Streptococcus sobrinus, 1132 .00% too 4 *90 0* 5 00 0 00 00 *0 SS Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in Genl~ank and Derwent databases.

L9UIZ-i) EF062-2J giJ47620 antigen 1 /11 [Streptococcus sobrinus) >pirIA603381A6033 8 surface 90E-33 -1

-I

109 2.IOE-34 EF062-2IpirIA35186IA35 salivary agglutinin receptor precursor Streptococcus EF06 -2 EF062-2 EF063-2 EF063-2 EF063-2 EF063-2

I

gil 11 097 1 gill00975 giJ47 049 6i1433 24 giI2 109266 .gi1l5 0 5 55 F-gJ1 47248 SspA [Streptococcus gordonii] SspA [Streptococcus gordonii] asal g ene product (AA l- 1296) [Enterococcus faecalis] aggregation substance (ASPI1) [Enterococcus faecalisl aggregation substance [Enterococcus faeciurn] aggregation substance [Plasmid pCFI10] >pirlH41662lH4l66 2 150K mating SspB precursor [Streptococcus gordonii] PAc protein precursor (AA -38 to 1527) [Streptococcus mutansi surface protein antigen precursor [Streptococcus sobrinusi cell surface antigen I/11 [Streptococcus mutans] irlS0683 9 1SO 68 39 SpaAendocarditis inununodominant antigen [Streptococcus sobrinus, antigen 1 /11 [Streptococcus sobrinus] >pirlA60338lA60 33 8 surface salivary agglutinin receptor precursor Streptococcus 109 110 3716 4003 5523 6338 110 107 3.80E-32 2.3012-21 0 0 0 0 9.90E-39 I .70E38 EF063-2 gnllPlDldIO15O 7_ EF063-2 gil47 267 EF063-2 bbs1148453 EF063-2 giJ 4 7 6 20 EF063-2 pirlA35 1861A35 132 5.OOE-36 107 6.5013-36 132 1.20E-35 F13-2 2.90E-35 109 2.I1OE-34 *0* 0*0 *00 0 0 4 0 0* 04 09 4 0 0 0 0 0 0 0 0 000 ~0e *0 *0 0 0 0 00 *0@00 *0 *4 0 0 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF063-2 EF063-2 EF064-2 EF064-2 EF064-2 EF064-2 EF064-2 EF064-2 gi109 71 11~l100975 SspA [Streptococcus gordoniil SspA [Streptococcus gordonij gil470 4 9 giJ43 324 giJ2 109266 gij 150555 gill 100973 gi1472 48 EF06,4-2gn IlPIDjI 1050 EF064-2~ 7_ asalI gene product (AA 1-1296) [Enterococcus faecalisl aggregation substance (ASPI1) [Enteocccus faecali s] aggregation substance [Enterococcus faeciurn] aggregation substance [Plasmid pCFI1O] >pirlH4l6621H41662 150K mating SspB precursor [Streptococcus gordoni] PAc protein precursor (AA -38 to 1527) [Streptococcus mutansi Surface protein antigen precursor [Streptococcus sobninus] cell surface antigen 1/11 [Streptococcus unutans] >p)irlS068 39 1SO 6839 SpaAendocarditis immunodominant antigen [Streptococcus sobrinus, antigen 1 /11 [Streptococcus sobrinus] >pirlA603381A603 3 8 surface salivary agglutinin receptor precursor Streptococcus SspA [Streptococcus gordoniil SspA [Streptococcus gordonii] T06D8. 1 [Caenorhabditis elegans] 110 110 3716 4003 5523 6338 110 107 132 107 3.80E-32 2.30E-21I 0 -0 0 0 9.90E-39 I1.70E-38 5 .OOE-36 [6.so36 EF064-2 1 bbs148453 13 .20E-35 EF064-2 EF064-2 giJ4 7620 pirlA35 186fA35 1321 109 110 110 137 2.90E-35 2. 1OE-34 3 .80E-32 2.30E-21I 8.50E- 17 qI EF064-2, gill1 0 09 7 1 EF064-21 gill 100 97 5 EF068-2 giJ790398 4 000 4 C@S C S 4 0 C C S. S C C

C

C

.o a

C.

4 C 0O 9

C**

C. Se C C **C*C eeC....

.4 4 C Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF068-2 gnIjP1D~d10208 inembrane glycoprotein [Equine herpesvirus 1] 210 5.80E-16 4 EF068-2 gi1228 6 204 (AFOI 1339) unknown [Acinetobacter calcoaceticus) 121 8.40E- 16 EF068-2 gi13 30862 membrane glycoprotein [Equine her-pesvirus 1] 208 >pirIH3 68021VGBEX EF068-2 gi117 0 7 24 7 partial CDS_[Caenorhabditis elegansl 131 3.70E- EF068-2 gn1lPIDId10208 membrane glycoprotein [Equine herpesvirus 1] 203 6.20E- 4 EF068-2 giJ2133 92 antifreeze glycoprotein [Notothenia coriieeps] 102 4.60E- 13 >pirlA3 84201A3 84 20 EF068-2 gnljP1Dje 125464 (AL022022) PORS-family protein [Mycobacteriuin 145 1.SOE- 12 EF068-2 giJ951 4 60 FIM-C.1 gene product [Xenopus laevis] >pir1A451551A45155 109 2.70E-12 mucm EF069-2 giJ79039 8 T06D8.1 [Caenorhabditis elegans] 137 8.50E- 17 EF069-2 gnlPIDIdlO208 membrane glycoprotein [Equine herpesvirus 1] 210 5.80E- 16 4 EF069-2 giJ22 862 04 (AFO 1133 9) unknown [Acinetobacter calcoaceticus] 121 -&.40E-16 EF069-2 gi13308 62 membrane glycoprotein [Equine herpesvirus 11 208 __________>pirIH368021VGBEX EF069-21 EF069-2 EF069g1117 7 247 gn~jPID~d 10208 4 giJ2 13392 partial CDS [Caenorhabditis elegans] zi 1)1 VLI membrane glycoprotein [Equine herpesvirus 1] 203 6.20E- antifreeze glycoprotein [Notothenia coriceps] 102 .4.60E-13 OO00 oS S0 065 0@ 000 S0O 96 00 .0 000 S .0.

0O 400 0 s 0 0 0 SO S0 5 0 0 Table 2. Closest match ing sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

8420 jA3 8420 EF069-2 gnljPIDje 125464 (AL022022) PGRS-family protein [Mycobacterium 145 1.50E-12 tuberculosis] ici EF069-2 giJ95 1460 FIM-C.1I gene product [Xenopus lacvis] >prA. 514 5 mucin I- i I 137 1 8.50E-17 EF070-2 EF070-2 EF070-2 EF070-2 EF07 0-2 EF070-2 EF070-2 gi179 0398 gnlPIDIdIO08 4 giJ228 6204 giJ33O862 gi1 1707247 gnlPIDId 10208 4 giJ2 13392 T06D8. 1 [Caenorhabditis elegans]_ membrane glycoprotein [Equine herpesvirus 1] 1' 210 ]5.80E- 16 I K 121 ~.quL-1c~ (AFOl 1339) unknown [Acinetobacter calcoaceticus] membrane glycoprotein [Equine herpesvirus I1I >pirIH368021VGBEX 1 partial CDS [Caenorhabditis elegans] membrane glycoprotein [Equine herpesvirus I1 208 1.1 0E- 1113.70E,15 6,20E- I t 4.60E-13 antifreeze glycoprotein [Notothenia coriiceps) >pirIA3 84201A3 8420 4.60E-13 EF070-2 guljPIDlel 25464 EF070-2 gi19514 6 0 EF07 1-2 gn1jP1Dje3064 28 EF071-21 giJ853751 EF073-21 gi143830 4 T 145 I .50E-12 (AL022022) PGRS-famnily protein [Mycobacterium 109

I

2.70E- 12 tuberculosis]_ FIM-C.1I gene product [Xenopus laevisl >pir1A451551A45 155 mucin unnamed protein product [Bacteriophage ri ti >gil 1353566 Lysin N-acetylinuramoyl-L- lanine amnidase [Bacteriophage AS1 11] xpaC [Bacillus subtilisi >gntIPlD~dlO05803 hydrolysis of 127 12.OOE-37 273 173 2.60E-36 7.I1OE- 16 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF074-2 giJ125669 8 chitinase [Serratia marcescens] >gill 2 5 669 8 chitinase [Serratia 618 2.60E-104 EF074-2 giJ17 639 85 chitmnase A [Vibrio harveyiJ 526 2.80E-84 EF075-2 giJ143 156 membrane bound protein (Bacillus subtilis] >gn1lPIDl 184471 593 1.70E-91 EF075-2 pirID7007OID7O transcriptional regulator homolog ywtF Bacillus subtilis 11 I 1I.90E-59 0 EF075-2 giJ1762 32 7 putative transcriptional regulator [Bacillus subtilis] 148 9.60E-53 EF075-2 giJ127 68 74 EpsA [Streptococcus thermophilus] 239 2.20E-33 EF075-2 gnlPIle28912 6 unknown [Streptococcus pneumoniael] 150 1.20E-27 EF075-2 giJ4852 75 putative regulatory protein [Streptococcus pneunloniae] 150 2.50E-27 EF075-2 g112 80 4 7 35 (AF030367) putative regulatory protein [Streptococcus 150 2.50E-27 prieumoniael EF075-2 gij280 4 7 47 (AF030369) putative regulatory protein [Streptococcus 150 2.5O -271 pneumfoniae) EF075-2 gnlIDle 116988 capsular polysaccharide synthesis protein [Streptococcus 148 5.30E-27 EF075-2 giJ280 4769 (AF030373) putative regulatory protein [Streptococcus 148 5.30E-27 Spneunioniael EF075-2 gill 147744 PSR [Enterococcus hirael 109 2. 1OE-23 EF075-2 giJ790 43 5 PSR [Enterococcus faeciuniI >pir1S541771S54177 PSR protein 102 4.4011-19 EF07 5-2 gi12 2672 39 ORF I [Staphylococcus epidermidis] 109 8.50E- 19 EF075-2 gnilPlDIdI189 membrane bound protein LytR [Synechocystis sp.] 121 2.80E-16 EF077-21gnlPIDIdl~l 13 cadmium-transporting ATPase [Synechocystis sp.] 396 12.30E-1 13 1 a..

a a a a..

a a. a a. a a. a. a a a Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF077-2 gillS 0 7 19 cadmium resistance protein [Plasmid p125 8 1 373 8.60E-1 12 >pirlA3256 I IA3256 1 EF077-2 gil1143753 cadmium-efflux ATPase [Bacillus firinus] >pirlD42707lD42707 361 8.1IOE-1 I I probable_____ EF077-2 giJ15297 8 ElI-E2 cadmium efflux adenosine triphosphatase 381 4.30E-1I EF077-2 gnljPIDle248808 unknown [Mycobacterium tuberculosis] 298 3.50E-107 EF077-21 giI4956 46 ATPase [Transpo son Tn5422] 361 12.1OE-106 EF077-2 EF077-2 EF077-2 EF077-2 EF077-2 EF077-2 EF077-2 EF077-2 EF077-2 EF078-2 EF078-2 gnllPIDI 18497 gil 1 990 49 gnllDlel 118603 gnllPIDe3O6540 gnljPIDje263525 _gilIPIDle264090 gnljPlDjdlOl 13 giJ177 3 1 66 similar to heavy metal-transporting AI rase LijacIIlus cadmium resistance protein [Latococcus lactis] similar to heavy metal-transportigATPase 352 254 3.60E-100 9.90E- 100

I

1* unknown [Mycobacterium tuberculosis] P-type ATPase [Mycobacteriun tuberculosis] 199 5.50AWS >gnl1PIDle2494 13 unknown [Mycobacterium tuberculosis] cadmium-transporting ATPase [Synechocystis sp.] probable copper-transporting atpase [Escherichia coli] 250 260

I

3 .OOE-84 L OOE-81 212 1-4.70E-80 >17 8 6 6 9 1 gi113 549 35 probable copper-transporting atpase Escherichia coli] -gil 143331 alkaline phosphatase regulatory Protein (Bacillus subtilis giJ4l0l 4 2 OR-FX18 [Bacillus subtilis] >gnljPDlel 185580 two-component sensor 212 257 235 8,5013-79 550E 58 M e

S

S

S

S

S

S..

S S S S S S *5 *S 5 5 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF078-2 gn1jPIDjd1lI 19 homologous to sp:PHORBACSU [Bacillus subtilis] j219 4.20E-44 EF078-2 gi1157 5578 histidine protein kinase [Thermotoga manitima] 191 7.10E-44 b~~~i~~~fbflp~~~IA Ai~ I~coocStcsceosi~ IAAF..d rTfl~O 'I ,.i1 1 Q')oof wstiliinp 1-inase r, actococcus lactis cremonsl EF078-2 6 i2 182992 Ihistidine, kinase [iLatoccu lacti rmsrm)... 152 EF078-2 gnlIDjdlOl 13J EF078-2~ EF078-2 EF078-2 EF078-2 EF078-2 gill 492 96 giJ581 1 88 gn1jP D~d 10 108 7 ffnlP1Dle2665 92 gi121 829 96 gnIlPIDd 101 113 giJ29 4893 gi1288 420 sensory transduction histidine kinase [Synechocystis sp-.

phosphate regulatory protein phoR (gtg start codon) [Kiebsiella phoR gene product (AA 143 1) [Escherichia coli] >gil6575 96 sensory transduction histidine kinase [Synechocystis sp.] 228 226 138 I I 7.

I OE-39 60E-33 60E-32 70E-32 unknown [Mycobacterium tuberculosis] 232 1. 1 Opal histidine kinase [Lactococcus lacti cremoris) 206 i 1.3012-3 1

I

EF079-2 giJ2O9 87 l 9 EF081-2 giJ467 80 6 EF081-2 gi1790 429 EF08 1-2 IgnlPIDtE2083 65 EF08 1-21 gi7 90433 sensory transduction histidine kinase [Synechocystis sp.] 1 phosp ate regulatory protein phoR (gtg start codon) [Shigella drug sensory protein A [Synechocystis PCC6803] >gnlPIDId 1017420 putative fimbrial -associated protein [Actinomyces naeslundii I penicillin-binding protein [Enterococcus faecalis] low affinity penicillin-binding protein 5 (PBP5) [Enterococcus penicillin-binding protein 5 [Enterococcus faccium] low affinity penicillin-binding protein 5 (PBP5) [Enerococcus 225 106 183 1356 1.6013-31 2.50E-31I 8 .60E-26 2. 10E-178 256 1.30E-3 I 607 1 .00E-78 604 604 L.IOE-78 2.70E-78

C

C C C C C C C C. C C C C C C C C C a. Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF081-2 gi(790 4 37 low affinity penicillin-binding protein 5 (PBP5) [Enterococcus 602 5. 1OE-78 EF081-2 giJ790 4 3 1 low affinity penicillin-binding protein 5 (PBP5) _[Enterococcus 591 2.60E-77 EF081-2 gi143342 D-alanyl-D-alanine carboxypeptidase [Enterococcus hirael 587 9.30E-77 EFO8I-2_ giJ49000 D-alanyl-D-alanine carboxypeptidase [Enterococcus hirae] 572 5.20E-74 EF081-2 gn1jPIDIl079 penicillin-binding protein 2 [Bacillus subtilisi 149 7.40E-24 4 EF081-2 gnlIPIle3l5088 MecAl [Staphylococcus sciuril IIl 4.40E- 19 EF081-2 gn1jPDje28665I MlecA protein [Staphylococcus sciuri] 106 2.90E-18 EF081-2 gnljPIDje3I658I MecA protein [Staphylococcus sciuri] Ill 2.90E- 18 EF081-2 gnlIPID16316607 MecA2 protein [Staphylococcus sciuri] 101 3.70E-14 EF081-2 glIjPIDe366l~I63 MecA protein [Staphylococcus sciuri] >gi4 6 6 1 3 mnecA gene 101 3.0-,1 EF083-2 giJ49 62 8 3 lysin [Bacteiophage Tuc2009] 436 6.20E,-176.

EF083-2 g!1530798 LysB [Bacteriophage phi-LC3] 421 3.OOE -175 EF083-21 gil 1 66 183 muramidase [Bacteriophage CP-71 186 1.20E-21 EF083-2 giJ1661 8 8 muramidase [Bacteriophage CP-91 >pirIJQ04381MUBPC9 188 5,OOE-21 EF083-2 giJ6 230 84 muramidase; muramidase [Bacteriophage LL-H] 193 8.40E-20 EF083-2 gi116617 5 muramridase [Bacteriophage CP-11 175 _3.40E- 19 EF083-2.gnlPIDle22 1272 lysozyme [Bacteriophage CP-1] >pirIA31086MUBPCP 175 3.4013-19 EF083-2 piriJQ04371MU N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) phage 171 9.5 0E- 19 EF083-2 giJ 4 10502 LysA [Bacteriophage inv4] >pir1538 47 7

IS

384 7 7 lytic enzyme 187 8.90E- 17 jlysA EF083-21 gi793850 Ilysin [Lactobacillus bacteriophage phi adh] >gnlPlDel2l73141 117 5.60E-l15 S S 9*

S

5 9 *9

S

'S S S S S

S..

ra S 0 0 0 0*0 0* 0 0 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in Genl~ank and Derwent databases.

lysin_ EF084-2 gi122933l 2 (A-1008220) YtfP [Bacillus subtilis] >gnIPlDjel 185879 similar 438 1.70E-140 to_ EF084-2, gi123672 34 (AE000425) hypothetical 43.8 kD protein in rhsB-pit intergenic 167 2.2013-51 EF084-2 giJ912464 No definition line found [Escherichia colij 167 6.OOE-51 EF084-2 gJPJ Djdl 1112 hypothetical protein [Synechocystis sp.] >pirlS76678lS76678 151 6.I1OE-42 EF084-2 gill 1573954 __hypothetical [Haemophilus influenzaej >pirjG64 161 IG64 161 142 2.9013-40 EF085-2 .gil 1209527 protein histidine kinase [Enterococcus faecalis] 2023 8.0013-279 EF085-2 giJ467057 phoR; B2168_C3_247 [Mycobacteriuin leprae] 226 8.8013-23 ___________>pirJS72905IS7290S____ EF085-2 gnIPlDjeI 19229 SenX3 [Mycobacterium bovis BCG) 222 3. 1 OM2 EF085-2 gnlIPlDje25 5152 unknown [Mycobacterium tuberculosis] >gn~jPIDje32 1546 222 3. 1 OE-22 EF085-2 giJ177 8 48 5 PcoS homnolog [Escberichia coli] >gi1178 6 78 3 (AE000162) R480; 1ll 3.8013-16 his EF085-2 gil 149296 phosphate regulatory protein phoR (gtg start codon) [Klebsiella 110 1.40E-14 EF085-2 giJ5811 88 plioR gene product (AA 143 1) [Escherichia coli] >gijI1 6 5 7 59 6 103 5.30E-14 EF085-2 giJ143331 alkaline phosphatase regulatory protein [Bacillus subtilisl 118 4.90E- 13 EF085-2. gil537239 alternate gene name phoM; CG Site No. 395 [Escherichia coli] 126 9.50E-13 EF085-2 giJ147 25 1 phoM [Escherichia coli] >giJ809670 phoM protein (I is 3rd 126 9.5013-13 in_ EF085-2 giJ2 182992 histidine kinase [Lactococcus lactis cremoris] '109 ,5.9013-12 S*S 559 5 5

S

9 9

S

9 9 S S 9* S S 9* S S S S S S S S S S S Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF086-2 giJ 4 37 7

O

6 alternative truncated translation product from E.coli 221 3.OOE-54 [Streptococcus EF086-2 giI437 7 05 hyaluronidase [Streptococcus pneumoniae] 221 1.60E-53 EF086-2 gi1595 847 hyaluronate lyase [Streptococcus agalactiae] 203 3.30E-44 1371A55 137 EF086-2 giJ70540 6 hyaluronate lyase [Staphylococcus aureusl 191 3.40E-42 EF086-2 giJ5 62 08 6 hyaluronidase [Propionibacterium acnes] 198 6.OOE-27 EF087-2 gi143 7706 alternative truncated translation product from E.coli 221 3.OOE-54 EF087-2 gil437705 hyaluronidase [Streptococcus pneumoniae] 221 1.60E-53 EF087-2 giJ59 584 7 hyaluronate lyase [Streptococcus agalactiae] 203 3.30E-44 1371A55 EF087-21 EF087-2 EF088-2 EF088-2 EF088-2 EF088-2 EF088-2~ EF091-2 EF091-2 giJ705 406 gil5620 86 gi143 7706 giJ4377 0 giJ595 847 giJ70540 6 giJ56 08 6 i155 60 1 6 hyaluronate lyase [Staphylococcus aureusj hyaluronidase [Propionibacterium acnes] 198 6.OOE-27 alternative truncated translation product from E-coli 221 3.OOE-54 [Streptococcus hyaluronate lyase [Streptococcus agalactiae] 203 5.30E-44 SpirIA55 137IA55 137 hyaluronate lyase [Staphylococcus aureus] 191 3.40E-42 1hyaluronidase (Propionibacteriuni acnes) 198 6.OOE-27 similar to plant water stespoen;OF Bclu utlj 189 2.40E- 17 I giJ235 3 3 j-~653 e-LEA [Caenorhabditis elegans] .1 9*9 9..

*99 9 0 9 9 9 99 9 9 9 9 99 99 9 99 9 9 9 9 9 9 99999 99 99 9 9 9 9999 99* .9 9. 9..

09 99 9 9 9 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF091-2 nIlIDle353216 seed maturation protein homolog [Arabidopsis thalianal 146 13.60E-1 II EFO91-2 gil 161171 late embryogenesis abundant protein [Picea glauca] 132 5.70E-1 I1 EF091-2 pirlS04909 1S049 embryonic protein DC8 (clone 8/10) carrot 127 6.5013-11I EF092-2 giJ268989 8 (AE000792) PTS system, cellobiose-specific IIB3 component 145 4.OOE-27 (celA)_ EF092-2 gn1IPDIdl0204 B. subtilis, cellobiose pho spho trans ferase system, celA; 116 1 .40E-26 EF096-2 giJ147329 transport protein [Escherichia coli] >gn1lPIDId 10 15409 532 2. 1OE-91 EF096-2 giJ1573 4 75 spermidine/putrescine-binding penipiasmic protein precursor 527 1.I1OE-79 EF096-2 giJ 1574803 spermidine/putrescine-binding periplasmic protein precursor 468 1.60E-75 EF096-2 gill 142681 Lpp38 [Pasteurella haemolytica) 446 4.40E' 72 EF096-2 gnlPID~d10152 Putrescine transport protein PotF [Escherichia coli] 216 1.50E-54 E-F096-2 gi11 4 73 34 periplasmic putrescine binding protein [Escherichia col. 216 2. 1OE-53 EF096-2 giJ26885 6 5 (AEOO1 165) spermidine/putrescine ABC transporter, 240 2.OOE-48 EF096-2 gi1188 1 733 PotD [Salmonella typhimurium] 253 !-70E-28 EF096-2 gn~fPID~d10192 spermidine/putrescine-binding penipiasmic protein 243 4.20E-26 6 EF096-2 gnljPlDje152543 potF gene product [Clostridium perfringens] 204 3.30E-21 E072 gii622991 mamiitol transport protein [Bacillus stearothermophilus] 547 4.90E-93 E972 giJ42 034 mannitol permease [Escherichia coli] >gii4 66 737 mannitol- 535 5.50E-85 9 9 999 9 9 9 *9 9 9* p p p a..

9 9 9 9 a 9 9 9 99 9 9 9 9 9 *9 999 99 9 9 9999999 9. 9. a. *9 9 9 9 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF097-2 giJ633650 enzyme 11(mannitol) [Staphylococcus carnosusl 516 2. 10E-82 193 jS22385 EF097-2 giJ882 462 protein-N(pi)-phosphohistidile-sugar phosphotransf'erase 509 3 .OOE-76 [Escherichia EF097-2 giJ3 12763 protein-N(pi)-pho sphohisti dine- ugar phosphotransferase 357 7.50E-70 EF097-2 gn1lPIDId096 homologue of mannitol transport protein of B. 492 3. 1 OE-62 6 EF097-2 gn]IPID~dl0079 mannitol-specific phophotransferase enzyme 11 [Bacillus 484 5.20E-61 2 EF097-2 gi1l 673 8 55 (AEOOOO2O) Mycoplasma pneumoniae, PTS system mannitol- 232 3.50E)59 specific EF097-2 gnllPIDId 10065 phosphotransferase enzymell, mannitol-specific [Mycoplasma 158 8.20E- 18 EF097-2 pirS7775715 7 77 phosphotransferase system enzyme 11 (EC 2.7.1.69), 103 2.OOE- 13 EF 10- 2 giJ2058 54 6 ComYC [Streptococcus gordonii] 193 7.30E-27 EFI100-2 gqZ20 5854 6 ComYC [Streptococcus gordoniii 193 -7.30E-27 EF 100-2 g11142 708 comnG3 gene product [Bacillus subtilis] >gnIlPIDjel 185739 150 2.90E-22 comOC EF 100-2 giJ1427 08 comG3 gene product [Bacillus subtilis] >gnlPlDIel 185739 150 2.90E-22 comGC EF 100-2 gi1148 4 3 7 secretory component [Erwinia chrysantheini] 134 4.40E- ___________>pirIE4702I1E4702 I pectic ft..

ft ft ft ,ftft ft ft ft q ft *4 ft 4 ft ft ft ft ft.

ft. ft ft ft ft ft. ft V ftftft ft.. ft.. ft ft ft ft ft ft. ft. ft ft ft ft ft. *ft ftftft' ft ft. ft. ft. ft ft. ft. ft ft ft Table 2. Closest matching sequences betwveen the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EFI100-2 gil 148 4 3 7 secretory component [Erwinia chrysanthemi] 134 4.40E- >pirIE4702 I IE4702 1 pectic____ EFI100-2 giJ60 62 62 OR.F_o 145 [Escherichia coli] >gif6937 06 HopG [Escherichia 136 9.I101- 13 EF 100-2 giJ6062 6 2 ORF o145 [Escherichia.coli] >gil6937 06 HopG [Escherichia 136 9.1IOE-113 coli] EF 100-2 gi1388 28 ExeG gene product [Aeromonas hydrophila] 132 3.50E-12 101149905 protein____ EFIOO-2 giJ38828 ExeG gene product [Aeromonas hydrophila] 132 3.50E-12 >pirIS2291011 4 9 9 0 5 protein____ EFI100-2 gnlIPIDleI 17259 etpG [Escherichia coli] 131 5.10E-12 EF 100-2 gnlIlDIel 17259 etpG [Escherichia coli] 131 5.101342 EFlQO-2 gil 42 18 9 outG gene product [Erwinia carotovora] >pirIS32 86 1 1S 3 286 1 130 9.90E- 12 outG____ EFIOO-2 gi1421 89 outG gene product [Erwinia carotovorai >PirIS 3 2 8 6

IS

3 2 8 6 1 130 9.90E- 12 outG____ EF 100-2 giJ609 62 8 putative [Vibrio cholerae] 128 1.60E-lI1 EF 100-2 giJ609 6 2 8 putative [Vibrio cholerael 128 1. 60E- I EFIOI-2 gnIjPlDIdlO257 bacG [Enterococcus faecalis] 106 3.60E- 17 [EF 101-2jgnIPIDIe32 1943 hypothetical protein [Enterococcus faecalis] >gnIIPlDje32 1943 105 1 .80E- 16 n :11 11-4 _1 EF 10L-2 EFi 110-2 gnljP1Dje1 18502 giJ4 333 8 similar to hypothetical proteins 11om 2. su"Iiws [L c us Staphylococcal serine proteinase homologue [Enterococcus faecalisl i462 2.30E- 195 1462 2.3013- 195 V V

V

V V 9 V 4 V

V

V

V..

V V V V 4** V V. *V V V V V *qV V V V Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in Genl~ank and Derwent databases.

EFi 10-2 gnIlPIDIdl100 10 glutamic acid specif ic protease prepropeptide [Staphylococcus 106 3.70E- 14 8 EFI 10-2 gi146687 _preproenzyme (AA -68 to 268) [Staphylococcus aureusl 106 6.70E- 14 EFI 11-2 giJ6060l 8 ORF_o783 [Escherichia coli] >gill 7 8 9 4 62 (AE000390) 477 8.10E-80 hypothetical 88.3 EFl121.2 gij2 6 26 826 YfkN [Bacillus subtilis] >gnlIPlDjel 182774 similar to 143 1.30E-96 EF 121-2 gi123 13187 (AE000532) 2',3'-cyclic-nucleotide 2-phosphodi sterase (cpdB) 413 2 .60E-82 EFl121-2 gil484 53 5'-nucleotidase [Vibfio parahaemolyticus] >gnlPIDId 100 1218 279 8.50E-47 EF121-2 giJ757842 UDP-sugar hydrolase [Escherichia coli] 239 1.60E-44 EFI21-2 gi1177 3 162 UDP-sugar hydrolase precursor [Escherichia coli] >gil 7 86 6 87 239 1.60E-44 EFl121-2 giJ 4 795O precursor polypeptide (AA -25 to 525) [Salmonella 229 2. 1OE-41 __________typhimurium] EF121-2 gi17 4 79 1 3 2',3'-cyclic-nucleotide 2'-phosphodiester-ase [Yersmnia, 115 4.70E -36 EF 121-2 gi16277 2 5'-nucleotidase [Discopyge ominata] >pirl S 19564 IS 195 64 137 5 .80E-35 nucleotidase EFl121-2 gi1157 3 5 73 2',3'-cyclic-nucleotide 2'-phospbodiesterase (cpdB) 114 8.90E-34 Haemophilus EFl121-21 gij537 0 54 2',3'-cyclic-nucleotide 2-phosphodiesterase [Eschenichia colid 110 IEIOE-31 EF 121 EF 121 EF 121 JEF 121 -2 bbs1135915 (12nu letds=gy rypos aidlioio-n e rti I. Ivr--Zy I iL -21 gi1l 7 374 43 15'-nuclcotidase [Boophilus microplus] 104 1.60E-28.

2 gi1202551 j5-nucleotidase precursor (EC 3.1.3.5) [Rattus norvegicus] 138 J6.1IOE-28 2 gi13 4 9 783 jecto-5'-nucleotidase [Mus musculus] >piriC2001 IJC2001 136 1. 1OE-27 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EFI121-2 gi123897 5'-nucleotidase [Homosapiens] >pirISlI 10321S1 11032 133 1.60E-27 nucleotidase EF122-2 giJ262 6 826 YfkN [Bacillus subtilis] >gnilPIDjel182774 simiflar to 143 1.30E-96 EF122-2 gil2313 187 (AE000532) 2',3'-cyclic-nucleotide 2'-phosphodiesterase (cpdB) 413 2.60E-82 EF122-2 gil 4 845 3 5'-nucleotidase [Vibrio parahaernolyticus] >gnlPIDIdlO01218 279 8.50E-47 EF 122-2 gi1757 842 UDP-sugar hydrolase [Escherichia coli] 239 1.60E-44 EF 122-2 giI177 3 162 UDP-sugar hydrolase precursor [Escherichida coli] >gil 17 866 87 239 1 .60E-44 EF 122-2 giJ47950 precursor polypeptide (AA -25 to 525) [Salmonella 229 2. 1OE-41 _______typhimurium] EF 122-2 gi174791 3 2',3'-cyclic -nucleo tide 2'-phosphodiesterase [Yersinia 115 4.70E-36 EF 122-2 giJ6277 2 5'-nucleotidase [Discopyge ommata] >pirlS 195641519564 137 5 .80E-35 nucleotidase EF 122-2 giJI57 3

S

7 3 2',3 -cycic-nuc eo tide 2'-phosphodiesterase (cpdB) 114 8.90E-34 EF 122-2 giJ53705 4 2',3'-cyclic-nucleotide 2'-phosphodiesterase [Escherichia coli] 110 1. 1 OE-3 1 EF 122-2 bbsj 135915 5-nucl eotidase=glycosylphosphatidy ifosi tol-ancho red protein 128 7.70E-29 EC_ EF122-2j gi11737 443 5'-nucleotidase [Boophilus microplus] 104 60E-28 EF 122-2 giJ20 2 551 5'-nucleotidase precursor (EC 3.1.3.5) [Rattus norvegicus] 138 6.1 OE-28 EF122-2 giJ3 497 83 ecto-5'-nucleotidase [Mus musculus] >pirJC2001 IJC2001 136 1LIOE-27 EF 122-2 gi123897 5'-nucleotidase [Homo sapiens] >pirjS I 10321S 11032 133 1.60E-27 Inucleotidase (EC 1F2-2~ 334 protein [Enterococcus faeciuml >pirlS055421S05542 630 9.4013-79 400.

90 9 9 9 9q 9 9 9 9. 9 9 .9.

:0 to, 9 t o 9 0 9 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBanlk and Derwent databases.

____________hypothetical EF 129-2 guS 12521 usp 45 gene product [Lactococcus lactis) >pirlJNOO971JNO097 374 1.30E-42 secreted EF 129-2 giJ149525 secreted protein [Lactococcus lactis] 371 3.60E-42 EF129-2 gnIPIDle3 13022 hypothetical protein [Bacillus subtilis] >gnlIPIDIl 186168 317 2.30E-33 EF130-2 gil48 8339 apa-amnylase [unidentified cloning vector] 621 6-70E-81 EF130-2 giJ488336 ORF [unidenti fled cloning vector] 242 8.OOE-27.

EF130-2 bbsll 12518 alpha-amylase {N-terminal region) [Artificial sequence, Peptide 237 4.80E-26 EF 130-2 gnllPIDie28914 4 ,yrpE [Bacillus subtilis] >gnljP1Diel 184540 ywpE [Bacillus 129 5.4013-11 EF131-2 gnlPIDle1185 2 8, penicillin-bindi-ng protein [Bacillus subtilis] 277 7.40E-43 EF 131-2 gii488330 alpha-amylase [unidenti fled cloning vector] 280 1.30E-31 EF 131-2 giJ509249 No definition line found [Lactobacillus plantarum] 274 1. 1 Ol$. EF 131-2 gnlPIDjdlO249 (AB009635) Fmt [Staphylococcus aureus] 170 5.60E-20 EF 131-2 guS 15050 DD-peptidase precursor [Streptomyces'lividans] 131 2.30E- 14 >pirlS48220IS48220_________ EFI131-2 gi11.534 4 8 serine DD-peptidase [Streptomyces lividans] 131 1.20E- 12 EF132-2 giJI53826 adhesin B [Streptococcus sanguis] >pirlA43583lA43583 adhesin 1257 2-.30E- 166 B EF 132-2 gil 1184932 ScbA [Streptococcus crista] 1248 3.70E-165 EF132'-2 gi31 0633 adhesin Streptococcus gordonii) 1247 5.I1OE- 165 EF132-2 gij393 2 69 adhesion protein [Streptococcus pneumoniae] 1204 3,40E- 163 EF 132-2, 1i575030 surface adhesin A precursor [Streptococcus pneumoniae] 1220 2.40E- 161 .6 '0 S

SS

Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF 132-2 giJlS 3834 adhesin specific for salivary pellicle of dental surfaces 120 4.80E-159 EF132-2 gill 117994 surface antigen A variant precursor [Streptococcus pneum niae] 1191 2.OOE-157 EF132-2 giJ49301 7 endocarditis specific antigen [Enterococcus faecalis] 931 3.70E-122 EF132-2,gnlPIDle255529 lipoprotein [Staphylococcus epidermidis] 453 3.20E-92 EF 132-2 gi112 4 54 64 YfeA [Yersinia pestis] >gi1 124 546 4 YfeA [Yersinia pestis] 364 3.60E-64 EF 132-2 gi1157 3330 adhesin B precursor (firnA) [Haemophiilus influenzae] 349 3.50E-63 EF 132-2 giJ755 07 5 periplasmic-bindinfg protein [Synechocystis sp.] 326 6.80E-62 I>gnllPIDld 10 18652 Mn EF 132-2 gnilPIle 118595 similar to ABC transporter (membrane protein) [Bacillus 174 3.10E-32 EF132-2 gi177 7933 TroA [Treponema pallidum]) 171 3.40E-32.

EF 132-2 gi1790 5 46 Tromp I [Treponema pallidum] 171 5.10E-32 Qey Derivent Derwent Gene Description BLAST BLAST IScore P-Value EF003-2 EF003-2 EF006-2 EF006-2 EF008-2 EF008-2 EF008-2 Access. INo.

W20909 W20 166 W20909 W2( 6 H. pylori outer membrane protein 14 ge 10705orfS.

Helicobacter pylori outer membrae potein, 1622 5006.aa.

H. pylori outer membrane protein l4gelO07O5orf5.

Hicobacter pylori outer membrane protein, 16225006.aa.

268 241 283 266 4J

I

R37495 W26367 R79--22 W22134 W20909 Pneumococcal fimbrial protein A. 967, Staphylococcus aureus saliva binding protein. 467 ~7 ROM precursor TROMPI. 181 Treponema pallidurn rare outer membrane protein -(TROM P-i1). 181 i H. pylori outer membrane protein l4gelO07O5orfS. 1319 1 .20E-39 .OOE-27 .20E-48 .10E-30 .2013-127 .50E-100 ~.OOE-36 ~.OOE-36 I .4E-53 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in Gen~ank and Derwent databases.

EF009-2 W20 166 Helicobacter pylori outer membrane protein, 16225006.aa. 278 2.50E-32 EFO 12-2 R48035 Hyaluronic acid synthase of Streptococcus eguisimilis. 227 3.20E-69 EF014-2 W 14070 S.thermophilus exopolysaccharide biosynthesis protein EpsR. 103 5.90E-19 EFO 14-2 W22 169 S.thermophilus exopolysaccharide synthesis operon epsA gene 103 7.30E-18 product.____ EFO 16-2 W 15799 Adherence factor 104R of Lactobacillus fermentum. 157 9.60E-22 EFO 16-2 W 15793 Adherence factor consensus sequence. 103 L.OOE-l 1 EF017-2 R48035 H-yaluronic acid synthase of Streptococcus eguisimilis. 241 8.90E-71 EF021-2 R31013 P39-alpha. 141 1.60E- 19 EF021-2 R33280 P39-beta. 134 7.OOE- 14 EF022-2 R48035 Hyaluronic acid synthase of Streptococcus eguisimilis. 324 2.2 0E -6 S EF03-2 R48035 Hyaluronic acid synthase of Streptococcus eguisimilis._ 155 9.90E 33 EF023-2 R70152 Streptococcus pneumoniae strain SPRU98 PIpA. 125 5.90E- 17 EF027-2 R48035 Hyaluronic acid synthase of Streptococcus equisimilis. 233 2.20E-34 EF028-2 W17830 Thermophilic alkaline phosphatase. 202 7.70E-59 EF028-2 Wi 1568 Excoli alkaline phosphatase mutant D I53FVQ329A. 182 7.90E-56 EF028-2 W 1570 E.coli alkaline phosphatase mutant D1I53H1K328H/Q329A. 182 7.90E-56 EF028-2 W26300 E.coli alkaline phosphatase mutant 182 I. 1OE-55 D I 3H/K3281Q329AJD330H.

I__

EF028-2 WI 11565 E.coli alkaline phosphatase mutant D IS3H/K328H/D330A. 182 3.1OE-55 EF028-2 WI 1557 E.coli alkaline phosphatase mutant D153H1D330N. 182 4.30E-55 EF028-2 W 11561 E.coli alkaline phosphatase mutant D153H/D330A. 182 4.30E-55 EF028-21 WI 1555 rs.coii alkaline phosphatase mutant D I53H1K328F1/D330N. 182 4.70E-55

A

Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF028-2 Wi 1566 Ecoli alkaline phosphatase mutant D1I531-11K3281D330L. 182 1.20E-54 EF028-2 WI 1569 E.coli alkaline phosphatase mutant K3281-/Q329A. 180 1.70E-54 EF028-2 W 11562 E.coli alkaline phosphatase mutant DlI 53H1D330L. 182 1 .7013-54 EF028-21 R26980 Fv(FRP5)-phoA recombinant antibody. 174 1 .90E--54 EF028-2 W 1567 E.coli alkaline phosphatase mutant Q329A. 179 2.30E-54 EF028-2 Wi 1558 E.coli alkaline phosphatase mutant K328H1D330N. 176 6.40E-54 EF028-2 WI 11563 E.coli alkaline phosphatase mutant K328H1D330A. 176 6.40E-54 EF029-2 R10044 Plasmid pO W3 60 encoded Human Growth Hormone (HGH) 320 3.50E-40 nuclease A EF029-2 R10041 Plasmid pOW350 nuclease A product. 320 4.30E-40 EF029-2 R73997 Staphylococcus aureus (Foggi) nuclease signal and mature 320 5.60E-40 _____sequences. EF029-2 R10043 Piasmid pOW36O encoding Human Growth Hormnone (HGH) 320 2.90E-38 nuclease EF030-2 R48035 H-yaluronic acid synthase of Streptococcus equisimilis. 277 6.l1OE-47 EF040-2 R59077 2-5A-dependent RNA-ase. 105 1.9013-18 EF040-2 W12703 Mouse 2-5A-dcpendent RNase. 105 1.90E- 18 EF040-2 R82661 Partial murine 2-5A-dependent RNase. 105 1 .9013-18 EF041-2 R48035 Hyaluronic acid synthase of Streptococcus equisimilis. 225 6.3013-26 EF054-2 R26042 P. yoelii SSP2 antigen. 286 8.0011-34 EF054-2 R85782 Group B Streptococcal mutant beta antigen without IgA binding 232 3.30E-24 Idomain. EF054-21 R85781 IGroup B Streptococcal wild-type beta antigen. 232 15.2 0 E-2 4 4** *49 44 4.

4 4 9 4 4 4 *4 4 0% 0 4*4 44. 4 4 4 4 444444 44 44 4 4 4 4444444 4444444 44 44 4 4 4 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF054-2 P91941 Sequence of preprospasmolysin. 204 3.I1OE- 19 EF054-2 W32519 Collagen-like polypeptide SEQ ID NO:2._ 180 7.50E- 18 EF054-2 Wl12324 Silver halide emulsion protein monomeric repeat unit 180 7.50E-18 EF054-2 W32522 Collagen-like polypeptide SEQ ID NO:5. 192 .I.60E-17 EF054-21 W12327 Silver halide emulsion protein monomeric repeat unit 192 1.60E- 17 EF054-2 W32520 Collagen-like polypeptide SEQ ID NO:3. 189 2.4013-17 EF054-2 W32532 Collagen-like polypeptide SEQ ID NO: 15. 189 2.40E-17 EF054-2 W 12325 Silver halide emulsion protein monomeric repeat unit 189 2.40E- 17 EF054-2 W12337 Silver halide emulsion protein monomeric repeat unit 15. 189 2.40E- 17 EF054-2 WI 12341 Silver halide emulsion FLAG(RTM)-tagged protein 189 2.60E- 17 EF054-2 W02098 S. mutans antigen 1/11. 161 5.40E., EF054-2 W02096 S. mutans antigen 1/11 fragment (aaS03-1 1114). 161 1.90E- 13 EF059-2 R26042 P. yoelii S5P2 antigen. 344 1 .90E-39 EF059-2 R85782 Group B Streptococcal mutant beta antigen without IgA binding 232 1.I1OE-26 EF059-2 R85781I Group B Streptococcal wild-type beta antigen. 232 1 .70E-26 EF059-2 P91941 Sequence of preprospasmolysin. 200 .1.50E-18 EF059-2 P60570 Sequence of the Falciparuin Interspersed Repeat Antigen 186 4.60E- 18 EF059-2 W02096 S. mutans antigen 1/11 fragment (aa803-1 114). 167 8.20E-16_ EF059-2 W02098 S. mutans antigen 1/11. -167 4.90E-l15 EF059-2 R79625 Endocarditis specific antigen region. 147 4.40E- 12 EF059-2 R26049 MSF precursor. 143 1.30E-lIl EF059-21 R28 150 ISugar beet chitinase 1. 148 et i a a a to. a *0 a Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF059-2 R26842 Protease from S. Aureus ATCC 12600. 147 2. 1OE-l II EF059-2 R79643 Inumunodominant antigen of Streptococcus sobrinus. 151 2. IOE-1 I EF059-2 W07539 Collagen like protein (CLP). 146 3.00E-1I1 EFO6I-2, R26042 P. ypelii SSP2 antigen. 241 1.70E-25 EFO61:-2 P60570 Sequence of the Falciparum Interspersed Repeat Antigen 199 1.6013-18 EF061-2 R85782 Group B Streptococcal mutant beta antigen without IgA binding 153 2.40E- 14 domain.

EF061-2 R85781 Group B Streptococcal wild-type beta antigen. 153 3.60E- 14 EF06 1-2 P91941 Sequence of preprospasmolysin. 163 9.70E- 14 EF061-2 P83194 Sequence of a bioadhesive precursor protein encoded by cDNA 156 7.90E- 13 ,clone EF061-2 R28 150 Sugar beet chitinase 1. 156 9. 1 OF-13 EF061-2 W02096 S. mutans antigen 1/11 fragment (aa803-1 114). 148 1.20t- 12 EF061-2 P82971 Bioadhesive precursor protein from cDNA 52. 148 9.70E- 12 EF061-2 W02098 S. mutans antigen 1/11. 148 1.50E-1l EF062-2 W02098 S. mutans antigen 1/11. 107 1.20E-36 EF062-2 R79643 Inunodominant antigen of Streptococcus sobrinus. 132 3.OOE-36 EF063-2 W02098 S. mutans antigen 1/11. 107 1.20E-36 EF063-2 R79643 Immunodominant antigen of Streptococcus sobrinus. 132 3.0012-36 EF064-2 W02098 S. mutans antigen 1/11. 107 1.20E-36 EF064-2 R79643 Immunodominant antigen of Streptococcus sobrinus.- 132 3.OOE-36 EF071-21 R85294 Phage R1-t LytR lysin. 127 3.70E-38_ EFO7I-21 R91515 lListeria phage lysin PLY51 1. 273 4.70E-37 0 £40 000 06 04 000 0 £00 0 00 0 0 0 0 0 00 0 0 0 *0 Ve*40 00600 00 00 0 0 0 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF075-2 W 14070 S.thermnophilus exopolysaccharide biosynthesis, protein EpsR. 239 4.20E-36 EF075-2 W22 169 'S.thermophilus exopolysaccharide synthesis operon epsA gene 239 4.OOE-34 ___product._ EF077-2_ R97280 Helicobacter-specific ATPase 439. 258 4.10E-74 EF077-2 R48036 Mycobacterium BCG immunogen. 192 2.20E-67 EF077-2 W06712 Helicobadter-specific ATPase 948 (ORF-4). 220 2.50E-67 EF077-2 R70419 Rat homologue of human Wilson disease gene ATP7I3. 186 9.80E-54 EF077-2_ R72343 Wilson disease protein ATP7B. 176 6.70E-40 EF077-2 R06376 Product of the ssclI gene. 166 3. IOE-28- EF077-2 R75396 Flea sodium pump alpha subunit. 146 2.40E-25 EF077-2 W20891 H. pylori transporter protein, l4ce22l9orfl. 15,6 8.60E- 14 EF078-2 R56667 Bacteroides fragLs RprX regulatory response protein. 148 9.308-18 EF078-2 R7 4630 Tomato TGETRI ethylene response protein. 130 7.80E- 13 EF078-2 R69849 Ethylene response (ETR) gene product. 128 1 .70E-1 1 EF078-2 R69850 Ethylene response (ETR)_mutant protein etrl 128 1 .70E- 11I EF078-2 R69851 Ethylene response (ETR) mutant protein etrl-2. 128 1. 70E-1 I1 EF078-2 R69852 Ethylene response (ETR) mutant protein etrl-3. 128 1.70E-1lI EF078-2 R69853 Ethylene response (ETR) mutant protein etrl-4. 128 1.70E-1 I EF078-2 R24296 Regulatory protein VanS involved in glycopeptide resistance. 142 2.70 E-1 1 EFO8I1-2 R27253 Penicillin binding protein PBP2A-epi. 101 4.70E- 16 EF081-2 R.27256 Penicillin binding protein PBP2A-27R. 101 6.OOE- EF081-2 R27257 Penicillin binding protein derivative N1. '101 6.20E- EF081-21 R27258 Penicillin binding protein derivative 1101 16.20E-15 0 0@*

OOS

0O 0O 0*e 0 0 0 0O 0 S 0 0 0 0 *0 0 0 0 S S 000 OSO See S S 0 5@eO@e 0@ Ge 0606050 SeeSSee S. Se 0 5 0 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

EF081-2 R27259 Penicillin binding protein derivative 101 6.2013-15 EF081-2 R27260 Penicillin binding protein derivative 101 6.20E- EF081-2 R27261 Penicillin binding protein derivative #15. 101 6.20E-lI5_ EBF081-2 R27263 Penicillin binding protein derivative V7. 101 6.20E- EF081-2 R27264 Penicillin binding protein derivative 101 6.20E- EF08 1-2 R27262 Penicillin binding protein derivative 101 6. 50E- EF08 1-2 R30845 Sequence encoded by the mec A gene.__ 101 6.90E- EF081-2 R27255 Penicillin binding protein PBP2A-27R. 101 6.90E- EF081-2 R31216 Penicillin binding protein PBP2A-27R. 101 7.OOE-15 EFI 110-2 R91042 V8 mature protease (aal-213). 106 6.60E- 16 EF 110-2 R91043 V8 mature protease (aa 1-2 14). 106 7.20,- 16 EFI 110-2 R91044 V8 mature protease (aa 1-215). 106 7.80E- 16 EF 10-2 R26842 Protease from S. Aureus ATCC12600. 106 6.70E- EF 110-2 R29644 Protease from S. Aureus. 106 1.20E- 14 EFI110-2 W2221 8 Protein encoded by pV8PPT(-) construct. 106 7.6013-14 EF 110-2 R91033 Beta-galactosidase-V8 protease fusion protein. 106 7.6013-14 EF 110-2 R9 1034 Beta-galactosidase-V8 protease fusion protein. 106 1 .70E- 13 EFi 10-2 W22219 Protein encoded by pV8D construct. 106 7.60E- 13 EF 110-2 R91035 Recombinant V8 protease V8D fusion protein. 106 7.60E- 13 EF 10-2 W22220 Protein encoded by pV8F construct. 106 7.90E- 13 EF 129-2. R14530 Usp45 protein. 374 2.40E-43 EF 129-21 R14 150 MSP encoded by pUCRS (DSM 5803). .372 4.70E-43 EF 131-21 R37495 Pneumococcal fimbrial protein A. 1185 6.80E-163 9 9 Table 2. Closest matching sequences between the polypeptides of the present invention and sequences in GenBank and Derwent databases.

E 3 121 E131-21 W26367 R79 722 W22 134 Staphylococcus aureus saliva binding protein. 41I8 ROM precursor TROMP 1. 171 Treponema, pallidurn rare outer membrane protein (TROMP-i 171 9.0013-31 288 TABLE 3. Conservative Amino Acid Substitutions.

Aromatic Hydrophobic Polar Basic Acidic Small Phenylalamne Tryptophan Tyrosine Leucine.

Isoleucine Valine Glutamine Asparagine Arginiie.

Lysine Histidine Aspartic Acid Glutainic Acid Alanine Serine Threonine Methionine Glycine a a too.

o.

289 T[able 4. Residues Comipri sing, Antigenic Epitope-Bearing Portion.

EFOO 1-2 from about Asp- 15 0 to about Lys- 15 2, from about Ser-256 to about Tyr- 259, from about Lys-360 to about Lys-363, from about Asn-406 to about EF002-2 from about Asp-8O to about Asp-83, from about Asp-281 to about Gly- 283.

EF003-2 from about Asn-263 to about GI -266.

EF004-2 from about Asn-23 to about Asn-26, from about Lys-83 to about Ser-87, from about Tyr- 154 to aboiteAsp -159.

EF005-2 from about Lys-249 to about Glu-252.

EF006-2 from about Gly-23 to about Asp-28.

EF008-2 from about Thr-92 to about Gly-94, from about Pro-161 to about Asp- 165, from about Gly-287 to about Thr-289.

F0 10-2 from about Pro- 129 to about Asn- 13 1.

F0 12-2 from about Asp-77 to about Asp-79, from about Asp-94 to about Lys-98, from about Asp-256 to about Thr-258, from about Glu-461 to about Asn- 468.

EFO 13-2 from about Thr-30 to about Asp-32, from about Glu-73 to about from about Gln-1 64 to about Asn- 166, from about Lys- 193 to about Gly- 195.

EFOI14-2 from about Ser-203 to about Asp-206, from about Gln-314 to about Gly- 316 F0 15-2 from about Pro-66 to about Gly-69.

EF016-2 from about Lys-236 to about Asn-239.

EFO 17-2 from about Ser-90 to about Gly-93, from about Thr- 197 to about Lys- 199, from about Lys-230 to about Asn-233, from about Ser-428 to about 1.

EF018-2 from about Lys- 159 to about Tyr- 16 1, from about Asn- 165 to about Ser- 167, from about Asn-250 to about Arg-256, from about Asn-392 to about from about Lys-416 to about Tyr-418, from about Asn428 to 290 Table 4. Residues Comprising Antigenic Epitope-Bearing Portion.

Arg-430.

EFO 19-2 from about Arg-209 to about Ser-21 1, from about Lys-287 to about Ser- 290.

EF020-2 from about Lys-57 to about Asn-62.

EF02 1-2 from about Ser-33 to about Gly-35, from about Glu-77 to about Gly-8 I, from about Asp-139 to about Lys-141, from about Glu-255 to about Ser- 258, from about Gln-271 to about Tyr-277.

EF023-2 from about Lys-232 to about Asp-234, from about Arg-304 to about Gly- 306, from about Thr-453 to about Arg-456, from about Ser-478 to about EF025-2 from about Arg- 183 to about Asp-] EF026-2 from about Ser-25 to about Asp-30, from about Asp-90 to about Asp-94, from about Gln- 107 to about Asn- I EF027-2 from about Gln-72 to about Lys-74, from about Lys-229 to about Asp- 23 1.

EF028-2 from about Asp-l 86 to about Gln-1 88.

EF029-2 from about Asp-i 18 to about Lys-1 22, from about Asp-1 24 to about Tyr- 12 6.

EF031-2 from about Glu-30 to about Gly-33.

EF034-2 from about Glu-25 to about Gly-27, from about Glu-75 to about Thr-77.

EF36-2 from about Gln- 177 to about Ser- 179.

EF037-2 from about Ser-25 to about Asp-30, from about Asp-90 to about Asp-94, about Gin-]107 to about Asn- I EF038-2 from about Asn-77 to about Lys-79, from about Tyr-88 to about Asn-92.

EF040-2 from about Lys-167 to about Gly-172, from about Lys-240 to about 291 Table 4. Residues Comprising Antigenic Epitope-Bearing Pordon.

EF044-2 from about Arg- 192 to about Gly- 194, from about Asn-200 to about.Asn- 203.

EF045-2 from about Asp-159 to about Asn-161, from about His-I 72 to about Gly- 174, from about Tyr-261 to about Gly-264, from about Lys-305 to about Glu-308.

EF046-2 from about Ser-I18 to about Gly-23, from about Gln-41 to about Ser-47, about Thr-76 to about Asp-78.

EF047-2 from about Asn-28 to about Asp-30, from about Asp-273 to about Asn- 277.

EF048-2 from about Asp-138 to about Lys-141, from about Asp-152 to about Gly-I 54.

1-2 from about Asp-73 to about Gly-76.

EF053-2 from about Ser-79 to about Gly-82.

EF055-2 from about Asp-26 to about Gly-28, from about Gln-67 to about Asp-69, from about Arg-71 to about Gly-74, from about Arg-87 to about Gly-89.

EF056-2 from about Arg-71 to about Gly-74, from about Arg-87 to about Gly-89.

EF058-2 from about Lys-129 to about Gly-133, from, about Gln-571 to about Tyrfrom about Pro-586 to about Gly-59 1.

EF065-2 from about Ser-236 to about Tyr-239, from about Asp-350 to about Gly- 352, from about Lys-415 to about Asn-4 18, from about Arg-446 to about Asp-448, from about Asn-489 to about Lys-49 1, from about Ser-S 16 to about Asp-S 18, from about Glu-639 to about Lys-642.

EF066-2 from about Ser-236 to about Tyr-239, from about Asp-350 to about Gly- 352, from about Lys-415 to about Asn-4 18, from about Arg-44 6 to about Asp-448, from about Asn-489 to about Lys-491, from about Ser-516 to Asp-SI 8, from about Glu-639 to about Lys-642.

EF067-2 from about Ser-236 to about Tyr-239, from about Asp-350 to about Gly- 352, from about Lys-415 to about Asn-4 18, fronm about Arg-446 to about Asp-448, from about Asn-489 to about Lys-49 I, from about Ser-5 16 to Asp-I 18, from about Gu-63 9 to about Lys-642.

9 Table 4. Residues Comprising Antigenic Epitope-Bearing Portion.

EF073-2 from about Met-98 to about Arg-100, from about Arg-l 10 to about Asp- EF074-2 from about Scr-53 to about Tyr-59, from about Ser-86 to about Gly-88, from about Pro-97 to about Gin- 100, from about Gln-230 to about Gly- 232.

EF076-2 from about Asn-38 to about Tyr-40, from about Asp-48 to about Asn-53, about Lys-79 to about Gly-8 1.

EF077-2 from about Arg-41 I to about Gly-4 13.

EF078-2 from about. Thr-294 to about Gly-296, from about Asp-366 to about Ginfrom about Glu-524 to about Gly-526.

EF080-2 from about Glu-1 64 to about Gly-1 66, from about Ser-206 to about Tyr- 208, from about Lys-239 to about Gi -243.

EF081-2 from about Asn-7 to about Ser- 11, from about Lys-77 to about from about Lys-1 12 to about Asn-1 114, from about Gly- 162 to about Aspfrom about Arg- 181 to about Gly- 183.- EF083-2 from about Gln-38 to about EF084-2 from about Lys-140 to about Asp-142, from about Gly-164 to about Argfrom about Arg-262 to about Gly-264.

EF085-2 from about Asn-95 to about Asp-97, from about Mrg-I 12 to about Asp- 114, from about Asp-258 to about Ser-260, from about Arg-401 to about EF086-2 from about Pro-I 12 to about Gly-I 15, from about Ser-222 to about Ser- 224, from about Asn-296 to about Gly-299, from about Thr-346 to about Lys-348, from about Asp-428 to about Ser-432.

EF087-2 from about Pro-I 12 to about Gly-1 15, from about Ser-222 to about Ser- 224, from about Asn-296 to about Gly-299, from about Thr-34 6 to about from about As -428 to about Ser-432.

EF088-2 from about Pro-I 12 to about Gly-I 15, from about Ser-222 to about Ser- 224, from about Asn-296 to about Gly-299, from about Thr-346 to about from about Asp-428 to about Ser-432.

293 Table 4. Residues Coi-iprising Antigenic Epitope-Bearing Portion.

EF090-2 from about Arg-2 to about EF091-2 from about Gln-40 to about Asp-43.

EF093-2 from about Lys-95 to about Gly- 9 7 EF094-2 from about Asp-3 14 to about Asp-3 16.

EF095-2 from about Ser-328 to about Thr-330, from about Asp-359 to about Asp- 363, from about Glu-637 to about Gly-639, from about Asn-744 to about EF096-2 from about Pro-128 to about Asn-130, from about Ser-193 to about Asp- 196.

EF097-2 from about Val-357 to about Gly-359.

EF099-2 from about Glu-44 to about Asp-47, from about Lys-] 154 to about Gly- 156, from about Asn-286 to about Asp-289.

EF 101-2 from about Lys-40 to about Asp-42, from about Pro-25 5 to about Asnfrom about Lys-288 to about Gly-290.

EF 102-2 from about Asp-314 to about Asp-3 16.

EF103-2 from about Asn-46 to about Gly-48.

EF 104-2 from about Pro-232 to about Lys-237, from about Ala-362 to about Asn- 366, from about Ser-42 1 to about Gly-423, from about Lys-488 to about Ser-490, from about Asp-S 50 to about Asn-5 52, from about Pro- 6 37 to about Lys-640, from about Asp-727 to about Gly-729, from about Asn- 751 to about Ser-754, from about Lys-771 to about Asn-774, from about lle-835 to about Asn-837, from about Pro-851 to about Gly-853.

EFi 05-2 from about Ser-40 to about Gly-43, from about Asn-94 to about Gln-97, from about Gln-220 to about Gly- 2 2 2 from about Asn-263 to about Gly- 265.

EF 106-2 from about Asp-72 to about Gly-75, from about Tbr-274 to about Aspfrom aboutAsn-3 10_to about Arg-3 13.

F77- 2 from about Thr- 15 5 to about Asn- 15 7, from about Thr- 189 to about Asp 294 Table 4. Residues Comprising Antigenic Epitope-Beanng Portion.

191, from about Arg-270 to about Gly-272, from about Thr-330 to about Lys-335, from about Asp-365 to about Asp-368, from about Pro-451 to Asp-453, from about Gly-485 to about Thr-488.

EFI 08-2 from about Lys- 142 to about Trp- 145, from about Thr- 147 to about Tyr- 150, from about Arg-212 to about Gly-214, from about Ser-248 to about Asp-251, from about Asp-384 to about Asp-387, from about Pro-481 to about Arg-483, from about Lys-491 to about Gly-494, from about Thr- 619 to about Gly-624, from about Asp-656 to about Asp-659, from about Lys-717 to about Asn-721, from about Ser-822 to about Gly-824, from about Tvr 1137 to about Thr-A 141.

S

S.

S

S

S

S*SS

S

S. S

S

S.

EFI 10-2 from about Pro-123 to about Gly-127, from about Thr-223 to about Gly- 225.

EF1 11-2 from about Lys-207 to about Asn-209, from about Asp-245 to about Asn-248, from about Lys-396 to about Asp-398, from about Glu-429 to Ser-432, from about Thr-470 to about His-474.

EFI 119-2 from about Asp-90 to about Asn-92, from about Gin- 142 to about Gly- EF121-2 from about Asn-159 to about Asp-161, from about Asn-35 1 to about Lys-353, from about Pro-658 to about Gly-660, from about Lys-786 to Ser-789.

EF122-2 from about Asri-159 to about Asp-161, from about Asn-35 I to about Lys-353, from about Pro-658 to about Gly- 66 O, from about Lys-786 to about Ser-789.

EF123-2 from about Asn-331 to about Arg-336, from about Asp-634 to about Gly- 636, from about Glu-780 to about Ser-782, from about Tyr-909 to about Asn-9 11, from about Lys-939 to about Glu-942, from about Asp- 1074 to about Gly-1076, from about Asp-1367 to about Gly-1369, from about Pro-1433 to about Lys-1435, from about Gly-1516 to about Asp-iS 18, from about Lys- 165 6 to about Asp- 1660, from about Lys- 18 6 0 to about Gln- 1863, from about Ser- 1916 to about Gin- 1919, from ab out Pro-i1940 about Gly-1942.

EF124-2 from about Asn-33 1 to about Arg-336, from about Asp-634 to about (ily- 636, from about Giu-780 to about Ser-782, from about Tyr-909 to about As-n-9 11, from about Lys-939 to about Glu-942, from about Asp- 1074 to About Gly- 107 6, from Aout Asp- 13 67 to about Gly- 13 69, from about to about Lys-1435, from about Gy-i 516 to about Asp-1518, 295 Table 4. Residues Comprising Antigenic Epitope-Bearing Portion.

ftrm about Lys-1 656 to about Asp- 1660, from about Lys- 1860 to about GI-n- 1863, from about SeT- 1916 to about Gln-1 919, fr-om about Pro-I 940 to about Gly- 1942.

EF125-2 from about Asn-331 to about Arg-336, from about Asp-634 to about Gl- 636, from about Glu-780 to about Ser-782, from about Tyr-909 to about Asn-91 1, from about Lys-93 9 to about Glu-942, from about Asp-] 074 to about Gly- 1076, from about Asp- 1367 to about Gly- 1369, from about Pro- 143 3 to about Lys- 143 5, from about Gly- 1516 to about Asp-IS5 18, from about Lys- 1656 to about Asp- 1660, from about Lys- 1860 to about GIn- 1863, from about SerI916. to about Gln- 1919, from about Pro- 1940 about Gly-1942.

EF 126-2 from about Ser-236 to about Tyr-239, from about Asp-350 to about Gly- 352, from about Lys-415 to about Asn-4 18, from about Arg-446 to about Asp-448, from about Asn-489 to about Lys-49], from about Ser-516 to about Asp-S 18, from about Glu-639 to about Lys-642.

EF127-2 from about Ser-236 to about Tyr-239, from about Asp-350 to about Gly- 352, from about Lys-4 15'to, about Asn-4 18, from about Arg-446 to about Asp-448, from about Asn-489 to about Lys-49 1, from about Ser-5 16 to about Asp-S 18, from about Glu-639 to about Lys-642.

EF128-2 from about Ser-236 to about Tyr-239, from about Asp-350 to about Gly- 352, from about Lys-415 to about Asn-418, from about Arg-446 to about Asp-448, from about Asn-489 to about Lys-49 1, from about Ser-S 16 to Asp-S 18, from about Glu-639 to about Lys-642.

EF129-2 from about Asn-300 to about Gly-302, from about Ser-316 to about Glyfrom about Asn-385 to about His-387 EF13 1-2 from about Lys-201 to about Tyr-204, from about Glu-263 to about Ser- 266.

EF132-2 from about Thr-26 to about Ser-28.

4* INDICATIONS RELATING TO A DEPOSITED MICROORGANISM (PCT Rule 13bis) Address of depositary institution (including postal code and country) 10801 University Boulevard Manasas, Virginia 201 10-2209 United States of America C. ADDITIONAL INDICATIONS (leave blank if not applicable) This information is continued on an additional sheet D. DESIGNATED STATES FOR WHICH INDICATIONS ARE MADE (if the indicarions are nor for all designated States) E. SEPARATE FURNISHING OF INDICATIONS (leave blank if not applicable) The indications listed below will be submitted to the International Bureau later (specify thec general nature of the indication. "A ccession Number of Depasit') For receiving Office use only This she Js eived with the international application SFor International Bureau use onlyV W This sheet was received by the international Bureau on: Authorized officer Authorized offiicer

Claims (25)

1. An isolated nucleic acid molecule comprising a polynucleotide having a nucleotide sequence selected from the group consisting of: a nucleotide sequence encoding any one of the amino acid sequences of the polypeptides shown in Table 1; or a nucleotide sequence complementary to any one of the nucleotide sequences in a nucleotide sequence at least 95% identical to any one of the nucleotide sequences shown in Table 1; or, a nucleotide sequence at least 95% identical to a nucleotide sequence complementary to any one of the nucleotide sequences shown in Table

2. An isolated nucleic acid molecule of claim 1 comprising a polynucleotide which hybridizes under stringent hybridization conditions to a polynucleotide having a nucleotide sequence identical to a nucleotide sequence in or of claim 1.

3. An isolated nucleic acid molecule of claim 1 comprising a polynucleotide which encodes an epitope-bearing portion of a polypeptide in of claim 1.

4. The isolated nucleic acid molecule of claim 3. wherein said epitope-bearing portion of a polypeptide comprises an amino acid sequence listed in Table 4.

5. A method for making a recombinant vector comprising inserting an isolated nucleic acid molecule of claim 1 into a vector.

6. A recombinant vector produced by the method of claim

7. A host cell comprising the vector of claim 6.

8. A method of producing a polypeptide comprising: growing the host cell of claim 7 such that the protein is expressed by the cell; and recovering the expressed polypeptide.

9. An isolated polypeptide comprising a polypeptide selected from the group consisting of: a polypeptide consisting of one of the complete amino acid sequences of Table 1; a polypeptide consisting of one the complete amino acid sequences of Table 1 except the N-terminal residue; a fragment of the polypeptide of having biological activity; and a fragment of the polypeptide of which binds to an antibody specific for the polypeptide of An isolated antibody specific for the polypeptide of claim 9.

11. A polypeptide produced according to the method of claim 8.

12. An isolated polypeptide comprising an amino acid sequence at least 95% identical to a sequence selected from the group consisting of an amino acid sequence of any one of the polypeptides in Table 1.

13. An isolated polypeptide antigen comprising an amino acid sequence of an E. faecalis epitope shown in Table 4.

14. An isolated nucleic acid molecule comprising a polynucleotide with a nucleotide sequence encoding a polypeptide of claim 9. A hybridoma which produces an antibody of claim

16. A vaccine, comprising: one or more E. faecalis polypeptides selected from the group consisting of a polypeptide of claim 9; and a pharmaceutically acceptable diluent, carrier, or excipient; wherein said polypeptide is present, in an amount effective to elicit protective antibodies in an animal to a member of the Enterococcus genus.

17. A method of preventing or attenuating an infection caused by a member of the Enterococcus genus in an animal, comprising administering to said animal a polypeptide of claim 9, wherein said polypeptide is administered in an amount effective to prevent or attenuate said infection.

18. A method of detecting Enterococcus nucleic acids in a biological sample comprising: contacting the sample with one or more nucleic acids of claim 1, under conditions such that hybridization occurs, and detecting hybridization of said nucleic acids to the one or more Enterococcus nucleic acid sequences present in the biological sample. -299-

19. A method of detecting Enterococcus faecalis nucleic acids in a biological sample obtained from an animal, comprising: a) amplifying one or more Enterococcus faecalis nucleic acid sequences in said sample using polymerase chain reaction, using primers selected from claim 1; and b) detecting said amplified Enterococcus faecalis nucleic acid. A kit for detecting Enterococcus antibodies in a biological sample obtained from an animal, comprising: a) a polypeptide of claim 9 attached to a solid support; and b) detecting means.

21. A method of detecting Enterococcus antibodies in a biological sample obtained from an animal, comprising: a) contacting the sample with a polypeptide of claim 9; and b) detecting antibody-antigen complexes. *l

22. An isolated nucleic acid molecule of claim 1 substantially as herein before described with reference to the Examples.

23. An isolated polypeptide according to claim 9 substantially as herein before described with reference to the Examples.

24. An isolated polypeptide according to claim 12 substantially as herein before described with reference to the Examples.

25. An isolated polypeptide antigen according to claim 13 substantially as herein before described with reference to the Examples.

26. A method according to claim 19 substantially as herein before described with reference to the Examples. 300

27. A method according to claim 21 substantially as herein before described with reference to the Examples. Dated this Twentieth day of July 2001. Human Genome Sciences, Inc. Wray Associates Perth, Western Australia Patent Attorneys for the Applicant S. 0 S S S. 0S 0S 0 SS OS S. S OS OS 0O S SOS S 5* S C OS. S S 550505 e.g. 0 0555 00 5000 S

555. S .555 e.g. S S *5 0 S Se S 00