AU6873196A - Extraction, amplification and sequential hybridization of fungal cell DNA and process for detection of fungal cells in clinical material - Google Patents

Extraction, amplification and sequential hybridization of fungal cell DNA and process for detection of fungal cells in clinical material

Info

Publication number
AU6873196A
AU6873196A AU68731/96A AU6873196A AU6873196A AU 6873196 A AU6873196 A AU 6873196A AU 68731/96 A AU68731/96 A AU 68731/96A AU 6873196 A AU6873196 A AU 6873196A AU 6873196 A AU6873196 A AU 6873196A
Authority
AU
Australia
Prior art keywords
fungal
dna
amplification
extraction
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
AU68731/96A
Other versions
AU717453B2 (en
Inventor
Hermann Einsele
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universitaetsklinikum Tuebingen
Original Assignee
Universitaetsklinikum Tuebingen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19530332A external-priority patent/DE19530332C2/en
Priority claimed from DE19530336A external-priority patent/DE19530336C2/en
Priority claimed from DE19530333A external-priority patent/DE19530333C2/en
Application filed by Universitaetsklinikum Tuebingen filed Critical Universitaetsklinikum Tuebingen
Publication of AU6873196A publication Critical patent/AU6873196A/en
Application granted granted Critical
Publication of AU717453B2 publication Critical patent/AU717453B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

Detecting fungal cells in clinical material, comprises extracting fungal DNA from whole blood and detecting the extracted fungal DNA, and/or identifying the fungal species from the extracted DNA. Also claimed is identifying a fungal species, comprising preparing a test soln. contg. at least segments of the DNA of the species to be identified, and detecting nucleotide sequences characteristic of the species in the soln.. Also claimed are the oligonucleotide probes (I)-(VI), TCTGGGTAGC CATTTATGGC GAACCAGGAC (I), TTCTGGCTAA CCCCAAGTCC TTGTGGCTTG (II), GTCTTTCCTT CTGGCTAGCC TCGGGCGAAC (III), GTTGGCCGGT CCATCTTTCT GATGCGTACT (IV), TTTCCTTCTG GCTAGCCTTT TTGGCGAACC (V), and CATGGCCTTC ACTGGCTGTG GGGGGAACCA (VI).
AU68731/96A 1995-08-17 1996-08-13 Extraction, amplification and sequential hybridization of fungal cell DNA and process for detection of fungal cells in clinical material Ceased AU717453B2 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
DE19530332A DE19530332C2 (en) 1995-08-17 1995-08-17 Extraction of fungal cell DNA and methods of detecting fungal cells in clinical material
DE19530336 1995-08-17
DE19530333 1995-08-17
DE19530332 1995-08-17
DE19530336A DE19530336C2 (en) 1995-08-17 1995-08-17 Sequential hybridization of fungal cell DNA and methods for detecting and identifying fungal cells in clinical material
DE19530333A DE19530333C2 (en) 1995-08-17 1995-08-17 Fungal cell DNA amplification and method for detecting fungal cells in clinical material
PCT/EP1996/003580 WO1997007238A2 (en) 1995-08-17 1996-08-13 Extraction, amplification and sequential hybridisation of fungus cell dna and a process for detecting fungus cells in clinical material

Publications (2)

Publication Number Publication Date
AU6873196A true AU6873196A (en) 1997-03-12
AU717453B2 AU717453B2 (en) 2000-03-23

Family

ID=27215385

Family Applications (1)

Application Number Title Priority Date Filing Date
AU68731/96A Ceased AU717453B2 (en) 1995-08-17 1996-08-13 Extraction, amplification and sequential hybridization of fungal cell DNA and process for detection of fungal cells in clinical material

Country Status (7)

Country Link
EP (2) EP0894870B1 (en)
JP (1) JP3803935B2 (en)
AT (1) ATE386817T1 (en)
AU (1) AU717453B2 (en)
DE (1) DE59611460D1 (en)
ES (2) ES2398047T3 (en)
WO (1) WO1997007238A2 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19635347C1 (en) * 1996-08-31 1997-12-18 Univ Eberhard Karls Oligo:nucleotide probes specific for fungal species
ES2621547T3 (en) 1999-05-28 2017-07-04 Fujirebio Europe N.V. Nucleic acid probes and methods for detecting clinically important fungal pathogens
DE10027113A1 (en) 1999-12-23 2001-09-27 Andreas Hoeft Method for determining microbial DNA / RNA, kit therefor and use of the method
DE10003580A1 (en) * 2000-01-28 2001-08-02 Univ Eberhard Karls Method, kit and DNA probes for the detection of a fungal species in clinical material
DE10048009A1 (en) * 2000-09-26 2002-04-11 Cytonet Gmbh & Co Kg Method of detecting fungal infections
WO2004053155A1 (en) 2002-12-06 2004-06-24 Roche Diagniostics Gmbh Multiplex assay detection of pathogenic organisms
JP4590573B2 (en) * 2006-02-21 2010-12-01 国立大学法人富山大学 Rapid identification of infection-causing bacteria
GB0621864D0 (en) * 2006-11-02 2006-12-13 Univ Manchester Assay for fungal infection
DE102007041864B4 (en) 2007-09-04 2012-05-03 Sirs-Lab Gmbh Method for the detection of bacteria and fungi
JP5599013B2 (en) * 2008-04-03 2014-10-01 キヤノン株式会社 Extraction method of microbial nucleic acid from blood sample
EP2325312A1 (en) 2009-11-19 2011-05-25 Qiagen GmbH Method for selective enrichment and isolation of microbial and optional additionally of viral nucleic acids
WO2011151473A1 (en) * 2010-06-02 2011-12-08 2B Blackbio S.L. Composition, method and kit for detecting fungi and yeasts by means of sequencing
PL219490B1 (en) * 2012-08-24 2015-05-29 Univ Jagielloński method for for isolating DNA of microorganisms from blood
US10994068B2 (en) 2015-01-26 2021-05-04 Stichting Sanquin Bloedvoorziening Methods and systems for the detection and removal of pathogens from blood
WO2019187240A1 (en) * 2018-03-28 2019-10-03 国立大学法人 富山大学 Method for rapid identification of candida in which incomplete match probes are used

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4130395A (en) * 1975-08-19 1978-12-19 Beth Israel Medical Center Process and apparatus for detection of specific biological factors by means of osmotic hemolysis
CA1317860C (en) * 1987-04-01 1993-05-18 Daniel Louis Kacian Techniques for preparing specimens for bacterial assays
ATE122103T1 (en) * 1988-03-28 1995-05-15 Amoco Corp DETECTION OF EUKARYOTIC MICROORGANISMS.
CA2025180A1 (en) * 1989-10-12 1991-04-13 William G. Weisburg Nucleic acid probes and methods for detecting pathogenic candida yeasts
US5284940A (en) * 1990-11-14 1994-02-08 Hri Research, Inc. Preparation for nucleic acid samples
CA2086136A1 (en) * 1991-08-15 1994-06-24 Luc Simon Dna probes for the detection of arbuscular endomycorrhizal fungi
EP0642588B1 (en) * 1992-05-18 2002-08-14 HOLMES, Ann Rachel Methods for the diagnosis of fungal infections
EP0574267A3 (en) * 1992-06-12 1994-08-24 Gen Probe Inc Preparation of nucleic acid from blood
US5426027A (en) * 1993-05-20 1995-06-20 The Government Of The United States Of America As Represented By The Secretary Nucleic acid probes and methods for detecting Candida DNA cells in blood
US5426026A (en) * 1993-09-15 1995-06-20 University Of Pittsburgh PCR identification of four medically important candida species using one primer pair and four species-specific probes
JP3608823B2 (en) * 1994-09-29 2005-01-12 株式会社東洋紡ジーンアナリシス Oligonucleotides for fungal detection and fungal species identification

Also Published As

Publication number Publication date
ATE386817T1 (en) 2008-03-15
EP0894870A3 (en) 1999-02-10
EP0846186A2 (en) 1998-06-10
WO1997007238A2 (en) 1997-02-27
WO1997007238A3 (en) 1997-07-17
ES2301170T3 (en) 2008-06-16
DE59611460D1 (en) 2008-04-03
JPH11508137A (en) 1999-07-21
EP0846186B1 (en) 2008-02-20
EP0894870A2 (en) 1999-02-03
JP3803935B2 (en) 2006-08-02
AU717453B2 (en) 2000-03-23
EP0894870B1 (en) 2012-10-10
ES2398047T3 (en) 2013-03-13

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Legal Events

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FGA Letters patent sealed or granted (standard patent)