AU665271B2 - Immunological method of analyses - Google Patents
Immunological method of analyses Download PDFInfo
- Publication number
- AU665271B2 AU665271B2 AU47053/93A AU4705393A AU665271B2 AU 665271 B2 AU665271 B2 AU 665271B2 AU 47053/93 A AU47053/93 A AU 47053/93A AU 4705393 A AU4705393 A AU 4705393A AU 665271 B2 AU665271 B2 AU 665271B2
- Authority
- AU
- Australia
- Prior art keywords
- antigen
- antibodies
- bound
- measured
- detected
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 238000000034 method Methods 0.000 title claims description 29
- 238000004458 analytical method Methods 0.000 title description 6
- 230000001900 immune effect Effects 0.000 title description 3
- 239000000427 antigen Substances 0.000 claims description 49
- 102000036639 antigens Human genes 0.000 claims description 49
- 108091007433 antigens Proteins 0.000 claims description 49
- 102000006395 Globulins Human genes 0.000 claims description 37
- 108010044091 Globulins Proteins 0.000 claims description 37
- 241000283973 Oryctolagus cuniculus Species 0.000 claims description 37
- 239000007791 liquid phase Substances 0.000 claims description 16
- 241000282414 Homo sapiens Species 0.000 claims description 15
- 238000011534 incubation Methods 0.000 claims description 14
- 238000001514 detection method Methods 0.000 claims description 12
- 239000007790 solid phase Substances 0.000 claims description 12
- 238000006243 chemical reaction Methods 0.000 claims description 9
- 238000002405 diagnostic procedure Methods 0.000 claims description 9
- 239000013060 biological fluid Substances 0.000 claims description 8
- 230000008105 immune reaction Effects 0.000 claims description 7
- 239000007787 solid Substances 0.000 claims description 7
- 238000005406 washing Methods 0.000 claims description 7
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 238000002372 labelling Methods 0.000 claims description 4
- 108010001336 Horseradish Peroxidase Proteins 0.000 claims description 2
- 239000000538 analytical sample Substances 0.000 description 13
- 239000000243 solution Substances 0.000 description 12
- 238000010521 absorption reaction Methods 0.000 description 10
- 239000003153 chemical reaction reagent Substances 0.000 description 10
- 102000013415 peroxidase activity proteins Human genes 0.000 description 10
- 108040007629 peroxidase activity proteins Proteins 0.000 description 10
- 241000283707 Capra Species 0.000 description 8
- JPMIIZHYYWMHDT-UHFFFAOYSA-N octhilinone Chemical compound CCCCCCCCN1SC=CC1=O JPMIIZHYYWMHDT-UHFFFAOYSA-N 0.000 description 7
- 239000000758 substrate Substances 0.000 description 7
- 150000001875 compounds Chemical class 0.000 description 6
- 239000013024 dilution buffer Substances 0.000 description 5
- 239000000872 buffer Substances 0.000 description 4
- 239000007853 buffer solution Substances 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000000523 sample Substances 0.000 description 4
- 230000001235 sensitizing effect Effects 0.000 description 4
- 239000012085 test solution Substances 0.000 description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 3
- 239000007983 Tris buffer Substances 0.000 description 3
- 229940098773 bovine serum albumin Drugs 0.000 description 3
- 238000006911 enzymatic reaction Methods 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 239000013641 positive control Substances 0.000 description 3
- 239000012086 standard solution Substances 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 210000001744 T-lymphocyte Anatomy 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 239000011888 foil Substances 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 239000001488 sodium phosphate Substances 0.000 description 2
- 229910000162 sodium phosphate Inorganic materials 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 2
- UAIUNKRWKOVEES-UHFFFAOYSA-N 3,3',5,5'-tetramethylbenzidine Chemical compound CC1=C(N)C(C)=CC(C=2C=C(C)C(N)=C(C)C=2)=C1 UAIUNKRWKOVEES-UHFFFAOYSA-N 0.000 description 1
- YRNWIFYIFSBPAU-UHFFFAOYSA-N 4-[4-(dimethylamino)phenyl]-n,n-dimethylaniline Chemical compound C1=CC(N(C)C)=CC=C1C1=CC=C(N(C)C)C=C1 YRNWIFYIFSBPAU-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 230000008629 immune suppression Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000001508 potassium citrate Substances 0.000 description 1
- 229960002635 potassium citrate Drugs 0.000 description 1
- QEEAPRPFLLJWCF-UHFFFAOYSA-K potassium citrate (anhydrous) Chemical compound [K+].[K+].[K+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O QEEAPRPFLLJWCF-UHFFFAOYSA-K 0.000 description 1
- 235000011082 potassium citrates Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CH2432/92 | 1992-08-03 | ||
| CH243292 | 1992-08-03 | ||
| PCT/EP1993/002045 WO1994003808A1 (en) | 1992-08-03 | 1993-07-30 | Immunological method of analyses |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU4705393A AU4705393A (en) | 1994-03-03 |
| AU665271B2 true AU665271B2 (en) | 1995-12-21 |
Family
ID=4233675
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU47053/93A Ceased AU665271B2 (en) | 1992-08-03 | 1993-07-30 | Immunological method of analyses |
Country Status (10)
| Country | Link |
|---|---|
| EP (1) | EP0606455A1 (en, 2012) |
| JP (1) | JPH07505479A (en, 2012) |
| AU (1) | AU665271B2 (en, 2012) |
| CA (1) | CA2120348A1 (en, 2012) |
| FI (1) | FI941502A7 (en, 2012) |
| NO (1) | NO941094D0 (en, 2012) |
| RU (1) | RU94020983A (en, 2012) |
| TW (1) | TW274582B (en, 2012) |
| WO (1) | WO1994003808A1 (en, 2012) |
| ZA (1) | ZA935616B (en, 2012) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1990015330A1 (en) * | 1989-06-01 | 1990-12-13 | The Ontario Cancer Institute | Immunoassay for determining the binding specificity of a monoclonal antibody |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4595661A (en) * | 1983-11-18 | 1986-06-17 | Beckman Instruments, Inc. | Immunoassays and kits for use therein which include low affinity antibodies for reducing the hook effect |
| US4788138A (en) * | 1987-04-30 | 1988-11-29 | Beckman Instruments, Inc. | Method to achieve a linear standard curve in a sandwich immunoassay |
-
1993
- 1993-07-27 TW TW082106006A patent/TW274582B/zh active
- 1993-07-30 EP EP93917713A patent/EP0606455A1/en not_active Ceased
- 1993-07-30 JP JP6504999A patent/JPH07505479A/ja active Pending
- 1993-07-30 WO PCT/EP1993/002045 patent/WO1994003808A1/en not_active Application Discontinuation
- 1993-07-30 RU RU94020983/14A patent/RU94020983A/ru unknown
- 1993-07-30 AU AU47053/93A patent/AU665271B2/en not_active Ceased
- 1993-07-30 CA CA002120348A patent/CA2120348A1/en not_active Abandoned
- 1993-08-03 ZA ZA935616A patent/ZA935616B/xx unknown
-
1994
- 1994-03-25 NO NO941094A patent/NO941094D0/no unknown
- 1994-03-30 FI FI941502A patent/FI941502A7/fi not_active Application Discontinuation
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1990015330A1 (en) * | 1989-06-01 | 1990-12-13 | The Ontario Cancer Institute | Immunoassay for determining the binding specificity of a monoclonal antibody |
Also Published As
| Publication number | Publication date |
|---|---|
| EP0606455A1 (en) | 1994-07-20 |
| AU4705393A (en) | 1994-03-03 |
| TW274582B (en, 2012) | 1996-04-21 |
| FI941502A7 (fi) | 1994-05-26 |
| ZA935616B (en) | 1994-02-03 |
| CA2120348A1 (en) | 1994-02-17 |
| NO941094L (no) | 1994-03-25 |
| FI941502A0 (fi) | 1994-03-30 |
| WO1994003808A1 (en) | 1994-02-17 |
| JPH07505479A (ja) | 1995-06-15 |
| NO941094D0 (no) | 1994-03-25 |
| RU94020983A (ru) | 1996-11-27 |
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