AU2022261882A1 - Materials and methods for improved phosphotransferases - Google Patents

Materials and methods for improved phosphotransferases Download PDF

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Publication number
AU2022261882A1
AU2022261882A1 AU2022261882A AU2022261882A AU2022261882A1 AU 2022261882 A1 AU2022261882 A1 AU 2022261882A1 AU 2022261882 A AU2022261882 A AU 2022261882A AU 2022261882 A AU2022261882 A AU 2022261882A AU 2022261882 A1 AU2022261882 A1 AU 2022261882A1
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AU
Australia
Prior art keywords
amino acid
seq
substitution
npt
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
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AU2022261882A
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English (en)
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AU2022261882A9 (en
Inventor
William Lloyd PERRY, III
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Janssen Biotech Inc
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Janssen Biotech Inc
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Publication date
Application filed by Janssen Biotech Inc filed Critical Janssen Biotech Inc
Publication of AU2022261882A1 publication Critical patent/AU2022261882A1/en
Publication of AU2022261882A9 publication Critical patent/AU2022261882A9/en
Abandoned legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01095Kanamycin kinase (2.7.1.95), i.e. neomycin-kanamycin phosphotransferase

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2022261882A 2021-04-21 2022-04-20 Materials and methods for improved phosphotransferases Abandoned AU2022261882A1 (en)

Applications Claiming Priority (17)

Application Number Priority Date Filing Date Title
US202163177749P 2021-04-21 2021-04-21
US202163177767P 2021-04-21 2021-04-21
US202163177759P 2021-04-21 2021-04-21
US202163177744P 2021-04-21 2021-04-21
US202163177746P 2021-04-21 2021-04-21
US202163177753P 2021-04-21 2021-04-21
US202163177764P 2021-04-21 2021-04-21
US202163177739P 2021-04-21 2021-04-21
US63/177,739 2021-04-21
US63/177,749 2021-04-21
US63/177,746 2021-04-21
US63/177,759 2021-04-21
US63/177,767 2021-04-21
US63/177,764 2021-04-21
US63/177,753 2021-04-21
US63/177,744 2021-04-21
PCT/US2022/025452 WO2022226005A1 (en) 2021-04-21 2022-04-20 Materials and methods for improved phosphotransferases

Publications (2)

Publication Number Publication Date
AU2022261882A1 true AU2022261882A1 (en) 2023-12-07
AU2022261882A9 AU2022261882A9 (en) 2023-12-14

Family

ID=83722653

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2022261882A Abandoned AU2022261882A1 (en) 2021-04-21 2022-04-20 Materials and methods for improved phosphotransferases

Country Status (11)

Country Link
US (1) US20240191215A1 (https=)
EP (1) EP4326747A4 (https=)
JP (1) JP2024516153A (https=)
KR (1) KR20230173159A (https=)
AU (1) AU2022261882A1 (https=)
BR (1) BR112023021960A2 (https=)
CA (1) CA3217224A1 (https=)
IL (1) IL307870A (https=)
MX (1) MX2023012514A (https=)
TW (1) TW202305127A (https=)
WO (1) WO2022226005A1 (https=)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7344886B2 (en) * 2002-11-29 2008-03-18 Boehringer Ingelheim Pharma Gmbh & Co., Kg Neomycin-phosphotransferase-genes and methods for the selection of recombinant cells producing high levels of a desired gene product
KR101149777B1 (ko) * 2002-11-29 2012-06-11 베링거 잉겔하임 파르마 게엠베하 운트 코 카게 신규한 네오마이신 포스포트랜스퍼라제 유전자 및 고생산성 재조합 세포의 선별 방법
US20080124760A1 (en) * 2006-07-26 2008-05-29 Barbara Enenkel Regulatory Nucleic Acid Elements
EP1975228A1 (en) * 2007-03-28 2008-10-01 Fachhochschule Mannheim Polynucleotides for enhancing expression of a polynucleotide of interest
AU2011342162B2 (en) * 2010-12-15 2017-04-20 Kyowa Kirin Co., Ltd. Method for producing proteins
US11389531B2 (en) * 2017-12-19 2022-07-19 Janssen Sciences Ireland Unlimited Company Methods and apparatus for the delivery of hepatitis B virus (HBV) vaccines

Also Published As

Publication number Publication date
CA3217224A1 (en) 2022-10-27
US20240191215A1 (en) 2024-06-13
IL307870A (en) 2023-12-01
TW202305127A (zh) 2023-02-01
JP2024516153A (ja) 2024-04-12
WO2022226005A1 (en) 2022-10-27
MX2023012514A (es) 2024-01-05
EP4326747A1 (en) 2024-02-28
EP4326747A4 (en) 2025-06-11
BR112023021960A2 (pt) 2023-12-26
KR20230173159A (ko) 2023-12-26
AU2022261882A9 (en) 2023-12-14

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