BR112023021960A2 - Materiais e métodos para fosfotransferases melhoradas - Google Patents

Materiais e métodos para fosfotransferases melhoradas

Info

Publication number
BR112023021960A2
BR112023021960A2 BR112023021960A BR112023021960A BR112023021960A2 BR 112023021960 A2 BR112023021960 A2 BR 112023021960A2 BR 112023021960 A BR112023021960 A BR 112023021960A BR 112023021960 A BR112023021960 A BR 112023021960A BR 112023021960 A2 BR112023021960 A2 BR 112023021960A2
Authority
BR
Brazil
Prior art keywords
npt
naturally occurring
proteins
methods
protein
Prior art date
Application number
BR112023021960A
Other languages
English (en)
Inventor
Lloyd Perry William Iii
Original Assignee
Janssen Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Janssen Biotech Inc filed Critical Janssen Biotech Inc
Publication of BR112023021960A2 publication Critical patent/BR112023021960A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01095—Kanamycin kinase (2.7.1.95), i.e. neomycin-kanamycin phosphotransferase

Abstract

materiais e métodos para fosfotransferases melhoradas. a presente invenção refere-se a proteínas de neomicina fosfotransferase (npt) de ocorrência não natural e sequências de ácido nucleico codificando tais proteínas npt. em uma modalidade específica, as proteínas npt que não ocorrem naturalmente têm atividade reduzida em relação à npt de tipo selvagem. as proteínas npt que não ocorrem naturalmente, aqui fornecidas, são úteis como um marcador selecionável para triagem transformado ou transfectado células. são também providos aqui vetores e kits compreendendo uma sequência de ácido nucleico codificadora de uma proteína npt de ocorrência não natural, e métodos de produção de células que expressam a proteína npt de ocorrência não natural e uma proteína de interesse ou uma sequência de rna não codificadora de interesse.
BR112023021960A 2021-04-21 2022-04-20 Materiais e métodos para fosfotransferases melhoradas BR112023021960A2 (pt)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US202163177767P 2021-04-21 2021-04-21
US202163177739P 2021-04-21 2021-04-21
US202163177759P 2021-04-21 2021-04-21
US202163177746P 2021-04-21 2021-04-21
US202163177744P 2021-04-21 2021-04-21
US202163177764P 2021-04-21 2021-04-21
US202163177753P 2021-04-21 2021-04-21
US202163177749P 2021-04-21 2021-04-21
PCT/US2022/025452 WO2022226005A1 (en) 2021-04-21 2022-04-20 Materials and methods for improved phosphotransferases

Publications (1)

Publication Number Publication Date
BR112023021960A2 true BR112023021960A2 (pt) 2023-12-26

Family

ID=83722653

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112023021960A BR112023021960A2 (pt) 2021-04-21 2022-04-20 Materiais e métodos para fosfotransferases melhoradas

Country Status (9)

Country Link
EP (1) EP4326747A1 (pt)
JP (1) JP2024516153A (pt)
KR (1) KR20230173159A (pt)
AU (1) AU2022261882A1 (pt)
BR (1) BR112023021960A2 (pt)
CA (1) CA3217224A1 (pt)
IL (1) IL307870A (pt)
TW (1) TW202305127A (pt)
WO (1) WO2022226005A1 (pt)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7344886B2 (en) * 2002-11-29 2008-03-18 Boehringer Ingelheim Pharma Gmbh & Co., Kg Neomycin-phosphotransferase-genes and methods for the selection of recombinant cells producing high levels of a desired gene product
US20080124760A1 (en) * 2006-07-26 2008-05-29 Barbara Enenkel Regulatory Nucleic Acid Elements
EP1975228A1 (en) * 2007-03-28 2008-10-01 Fachhochschule Mannheim Polynucleotides for enhancing expression of a polynucleotide of interest
EA202091517A1 (ru) * 2017-12-19 2020-11-03 Янссен Сайенсиз Айрлэнд Анлимитед Компани Способы и устройство для доставки вакцин против вируса гепатита b (hbv)

Also Published As

Publication number Publication date
EP4326747A1 (en) 2024-02-28
IL307870A (en) 2023-12-01
JP2024516153A (ja) 2024-04-12
AU2022261882A9 (en) 2023-12-14
KR20230173159A (ko) 2023-12-26
CA3217224A1 (en) 2022-10-27
TW202305127A (zh) 2023-02-01
AU2022261882A1 (en) 2023-12-07
WO2022226005A1 (en) 2022-10-27

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