AU2021383756A1 - Methods and systems for mechanoporation-based payload delivery into biological cells - Google Patents

Methods and systems for mechanoporation-based payload delivery into biological cells Download PDF

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Publication number
AU2021383756A1
AU2021383756A1 AU2021383756A AU2021383756A AU2021383756A1 AU 2021383756 A1 AU2021383756 A1 AU 2021383756A1 AU 2021383756 A AU2021383756 A AU 2021383756A AU 2021383756 A AU2021383756 A AU 2021383756A AU 2021383756 A1 AU2021383756 A1 AU 2021383756A1
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AU
Australia
Prior art keywords
cell
processing
processing apparatus
cells
inlet
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Abandoned
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AU2021383756A
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English (en)
Inventor
Alexander Alexeev
Sewoon HAN
Ockchul KIM
Ian SICHER
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CellFE Inc
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CellFE Inc
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Filing date
Publication date
Application filed by CellFE Inc filed Critical CellFE Inc
Publication of AU2021383756A1 publication Critical patent/AU2021383756A1/en
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Cell Biology (AREA)
  • Mechanical Engineering (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AU2021383756A 2020-11-18 2021-11-18 Methods and systems for mechanoporation-based payload delivery into biological cells Abandoned AU2021383756A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063115507P 2020-11-18 2020-11-18
US63/115,507 2020-11-18
PCT/US2021/059856 WO2022109113A1 (en) 2020-11-18 2021-11-18 Methods and systems for mechanoporation-based payload delivery into biological cells

Publications (1)

Publication Number Publication Date
AU2021383756A1 true AU2021383756A1 (en) 2023-06-22

Family

ID=81709779

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2021383756A Abandoned AU2021383756A1 (en) 2020-11-18 2021-11-18 Methods and systems for mechanoporation-based payload delivery into biological cells

Country Status (9)

Country Link
US (2) US11788050B2 (cg-RX-API-DMAC7.html)
EP (1) EP4232554A1 (cg-RX-API-DMAC7.html)
JP (1) JP7642813B2 (cg-RX-API-DMAC7.html)
KR (1) KR102819986B1 (cg-RX-API-DMAC7.html)
CN (1) CN117015596B (cg-RX-API-DMAC7.html)
AU (1) AU2021383756A1 (cg-RX-API-DMAC7.html)
CA (1) CA3201944A1 (cg-RX-API-DMAC7.html)
IL (1) IL302976A (cg-RX-API-DMAC7.html)
WO (1) WO2022109113A1 (cg-RX-API-DMAC7.html)

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US12227729B2 (en) 2020-12-24 2025-02-18 Cellfe, Inc. Methods and systems for high-throughput cell processing
CN115254214B (zh) * 2022-06-29 2023-07-25 中国科学院精密测量科学与技术创新研究院 一种微流控通道、微流控芯片和生化分子递送方法
US20250290023A1 (en) * 2024-03-15 2025-09-18 Cellfe, Inc. High-Throughput Mechanoporation for Scalable and Cost-Effective Cell Therapy Manufacturing

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Also Published As

Publication number Publication date
US20230416781A1 (en) 2023-12-28
IL302976A (en) 2023-07-01
US20230174918A1 (en) 2023-06-08
JP2023550090A (ja) 2023-11-30
US12359158B2 (en) 2025-07-15
EP4232554A1 (en) 2023-08-30
JP7642813B2 (ja) 2025-03-10
KR102819986B1 (ko) 2025-06-12
KR20230110308A (ko) 2023-07-21
US11788050B2 (en) 2023-10-17
CA3201944A1 (en) 2022-05-27
CN117015596B (zh) 2024-02-09
CN117015596A (zh) 2023-11-07
WO2022109113A1 (en) 2022-05-27

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