AU2021243743B2 - Aptamers that bind thiamine analogs and derivatives - Google Patents

Aptamers that bind thiamine analogs and derivatives

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Publication number
AU2021243743B2
AU2021243743B2 AU2021243743A AU2021243743A AU2021243743B2 AU 2021243743 B2 AU2021243743 B2 AU 2021243743B2 AU 2021243743 A AU2021243743 A AU 2021243743A AU 2021243743 A AU2021243743 A AU 2021243743A AU 2021243743 B2 AU2021243743 B2 AU 2021243743B2
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Australia
Prior art keywords
aptamer
aryl
alkyl
sequence
cycloalkyl
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AU2021243743A
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AU2021243743A1 (en
Inventor
Alexandria FORBES
Xuecui GUO
Ji-In Kim
Kevin G. LIU
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Meiragtx Gene Regulation Ltd
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Meiragtx Gene Regulation Ltd
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    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
    • C—CHEMISTRY; METALLURGY
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    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/11—Applications; Uses in screening processes for the determination of target sites, i.e. of active nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10041—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/15043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2021243743A 2020-03-24 2021-03-24 Aptamers that bind thiamine analogs and derivatives Active AU2021243743B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202062994135P 2020-03-24 2020-03-24
US62/994,135 2020-03-24
PCT/IB2021/000163 WO2021191680A1 (en) 2020-03-24 2021-03-24 Aptamers that bind thiamine analogs and derivatives

Publications (2)

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AU2021243743A1 AU2021243743A1 (en) 2022-11-24
AU2021243743B2 true AU2021243743B2 (en) 2025-08-07

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AU2021243743A Active AU2021243743B2 (en) 2020-03-24 2021-03-24 Aptamers that bind thiamine analogs and derivatives

Country Status (12)

Country Link
US (1) US12570988B2 (https=)
EP (1) EP4127176A1 (https=)
JP (1) JP7542644B2 (https=)
KR (1) KR102879552B1 (https=)
CN (1) CN115698292A (https=)
AU (1) AU2021243743B2 (https=)
BR (1) BR112022019284A2 (https=)
CA (1) CA3172864A1 (https=)
IL (1) IL296711B2 (https=)
MX (1) MX2022011940A (https=)
PH (1) PH12022552557A1 (https=)
WO (1) WO2021191680A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3239306A1 (en) * 2021-12-15 2023-06-22 Xuecui GUO Aptamers and small molecule ligands
CN117417301A (zh) * 2022-01-26 2024-01-19 上海日馨医药科技股份有限公司 含硫类化合物、其药物组合物及应用
EP4599064A1 (en) * 2022-10-07 2025-08-13 Okinawa Institute of Science and Technology School Corporation An rna aptamer that binds to asp7967 or an analogue thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012032522A1 (en) * 2010-09-07 2012-03-15 Yeda Research And Development Co. Ltd. Thiamine pyrophosphate (tpp) riboswitch mutants producing vitamin b1 enriched food and feed crops

Family Cites Families (5)

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Publication number Priority date Publication date Assignee Title
KR102699584B1 (ko) 2015-02-02 2024-08-28 메이라지티엑스 유케이 Ii 리미티드 대안적 스플라이싱의 압타머 매개 조절에 의한 유전자 발현 제어
ES2954929T3 (es) 2016-02-02 2023-11-27 Meiragtx Uk Ii Ltd Regulación de expresión génica mediante control mediado por aptámeros de ribozimas de autocorte
AU2017213835C1 (en) 2016-02-02 2024-04-18 MeiraGTx Gene Regulation Limited Regulation of gene expression through aptamer-modulated polyadenylation
ES3024480T3 (en) 2017-02-21 2025-06-04 Meiragtx Uk Ii Ltd Regulation of gene expression by aptamer-mediated accessibility of polyadenylation signals
SG11201908043UA (en) 2017-03-02 2019-09-27 Meiragtx Uk Ii Limited Regulation of gene expression by aptamer-modulated rnase p cleavage

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012032522A1 (en) * 2010-09-07 2012-03-15 Yeda Research And Development Co. Ltd. Thiamine pyrophosphate (tpp) riboswitch mutants producing vitamin b1 enriched food and feed crops

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
EDWARDS T et al., 'Crystal Structures of the Thi-Box Riboswitch Bound to Thiamine Pyrophosphate Analogs Reveal Adaptive RNA-Small Molecule Recognition', STRUCTURE. (2006), vol. 14, no. 9, pages 1459-1468. *

Also Published As

Publication number Publication date
US20230265441A1 (en) 2023-08-24
BR112022019284A2 (pt) 2023-02-14
MX2022011940A (es) 2023-01-05
PH12022552557A1 (en) 2024-04-22
JP2023519266A (ja) 2023-05-10
WO2021191680A1 (en) 2021-09-30
IL296711B2 (en) 2025-09-01
JP7542644B2 (ja) 2024-08-30
US12570988B2 (en) 2026-03-10
CN115698292A (zh) 2023-02-03
IL296711B1 (en) 2025-05-01
EP4127176A1 (en) 2023-02-08
IL296711A (en) 2022-11-01
KR102879552B1 (ko) 2025-11-10
KR20230019816A (ko) 2023-02-09
AU2021243743A1 (en) 2022-11-24
CA3172864A1 (en) 2021-09-30

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