AU2021105916A4

AU2021105916A4 - A Traditional Chinese Medicine Composition For Treating Aeromonas Hydrophila Disease Of Ducks

Info

Publication number: AU2021105916A4
Application number: AU2021105916A
Authority: AU
Inventors: Yanan Cai; He MA; Xiangzhu MENG; Hongbo NI; Yuzhe SUN; Xinbo YANG; Quan Zhao
Original assignee: Jinlin Agricultural Univ; Qingdao Agricultural University
Current assignee: Jinlin Agricultural University; Qingdao Agricultural University
Priority date: 2021-08-19
Filing date: 2021-08-19
Publication date: 2021-10-21
Anticipated expiration: 2029-08-19

Abstract

The present invention discloses a traditional Chinese medicine composition for treating aeromonas hydrophila disease of ducks, which comprises components by weight as follows: 40-60 parts of Discolor Cinquefoil Herb, 40-60 parts of Rhizoma Paridis and 35-55 parts of Rabdosia Rubescens, 35-55 parts of Hibiscus, 30-50 parts of Pericarpium Granati, 30-50 parts of Polygonum Hydropiper L, 30-50 parts of Chinese Gall, 30-50 parts of Garden Burnet, 25-45 parts of Yerbadetajo Herb, 25-45 parts of Herba Agrimoniae, 25-45 parts of Radix Bupleuri , 20-40 parts of Fructus Chebulae, 20-40 parts of Fructus Mume, 20-40 parts of Cherokee Rose, 20-40 parts of Fructus Aristolochiae, 20-40 parts of Greater Celandine Herb, 15-35 parts of Flos Caryophyllata, 15-35 parts of Szechwan Chinaberry Fruit, and 15-35 parts of Rhizoma alismatis. In addition, some clinic-controlled trials have demonstrated that the cure rate of this prescription for treating the aeromonas hydrophila disease of ducks reached 94.20%, while the effective rate was 97.81%.

Description

Descriptions

A Traditional Chinese Medicine Composition for Treating aeromonas

hydrophila disease of Ducks

Technical Field The present invention relates to a traditional Chinese medicine composition for treating poultry diseases, which belongs to the field of animal medicine, particularly to a traditional Chinese medicine composition used for treating aeromonas hydrophila disease of ducks exclusively.

Background Technology Aeromonas hydrophila can be found in fresh water, soil and sewage in nature easily, which would make aquatic animals, including fish, shrimps, and waterfowls, get disease through infection, and belongs to a conditional pathogenic bacterium. Aeromonas hydrophila disease is an acute and septicemic infectious disease which would mainly attack through infection of digestive canal. This disease is very similar to duck viral hepatitis either in terms of onset days, symptoms or pathological changes. However, the diseased ducks showed obvious lung lesions, including pulmonary hemorrhage, pulmonary edema, being in purple black, and there were hemorrhagic bubbles exudated from the cut section. In addition, combined with the clinical respiratory distress syndrome, there were relatively more bacteria seen on the microslide of lung, which can be utilized to complete preliminary diagnosis, however, it is necessary to conduct laboratory examinations to make a definite diagnosis. The outbreak of disease is common throughout the country, while the incidence rate is increasing year by year, and the mortality rate is relatively high. Thus, it would cause serious losses to farmers and has become a new problem for the duck industry. 1. Epidemiology Aeromonas hydrophila can be easily found in water environments including lakes, ponds, and sludge, etc., which is incredibly easy to attack fish and frogs. Furthermore, the fish ponds for breeding ducks are full of nutriments, which are conducive to the growth and reproduction of Aeromonas hydrophila. Ducks would be infected when swimming in the water or have eaten diseased or infected fish and frogs. In addition, the onset season is in summer, and ducks at different days of age are all susceptible to infection. Furthermore, the mortality rate is relatively high, which is between 20% and 60% approximately. 2. Clinical symptoms and pathological changes The symptoms of diseased ducks include being lassitude, loss of appetite or

Descriptions

anorexia, necking down and standing transfixed to the ground, being unwilling to walk, closing eyes and showing lethargy, having breathing difficulties, and keeping mouth breathing as well as being accompanied by wheezing. For those infected severely, the symptoms showed including lying prone and paralysis, etc., as well as being unwilling to swim. Furthermore, some of them who are willing to swim cannot move due to suffering from paralysis of both legs. In addition, the symptoms include having loose bowels in gray-white or light-green frequently, wherein, some diseased ducks would be stained with mushy stool on feathers around anus. During the later stages of the disease, such diseased ducks would exhibit symptoms of being keen on lying down, or suffering from paralysis of both legs, moving slowly, or becoming ataxia. Finally, these ducks would flop down and their legs present paddling gestures or opisthotonos. In addition, most diseased ducks would show hematomas or red appearance on the shin or toe of one side. Furthermore, it can be seen that there was a small amount of red ascites in the abdominal cavity, and the hemorrhage occurred in internal organs through the autopsy of certain cases; meanwhile, the liver, which was earthy yellow, was enlarged and covered with hemorrhagic spots or blood spots densely; and the lung, which was dark purple, was enlarged and accompanied with diffuse hemorrhage, in addition, there was red foamy liquid flowing out from the cut section. Furthermore, the fatty hemorrhage occurred on coronary plexus, and there were various blood spots of different sizes appeared in the serosae of heart; meanwhile, the spleen was swollen, accompanied with stagnation of the blood and hemorrhage; as well as the intestinal mucous membrane fell off, and the diffuse hemorrhage appeared; furthermore, the kidney exhibited stagnation of the blood or hemorrhage; and the ring hemorrhage appeared in tracheal mucosa. Meanwhile, the tibiotarsus, and the femoro-tibial and tibio-metatarsal joints were swollen, and there was dark red blood flowing out from the toes, even some would suffer from muscle necrosis of legs. 3. Existing prevention measures Modem veterinary medicine mainly uses various antibiotics to treat such disease. Unfortunately, the therapeutic effect of such method is unsatisfactory for the reason of the existence of drug resistance, while the cost is high, and has many toxic and side effects. In addition, it would make the amount of drug residues in ducks increase. Although, the newly launched immune-enhancer, probiotics and vaccines, etc. would play some effect on the prevention and control of Aeromonas hydrophila disease of ducks, the application of which has been restricted due to their inherent limitations of usage, the irritation to organisms, and the complexity of operation, and thus it is deemed as one of the critical factors that increased the

Descriptions

difficulty of effective control and radical treatment of Aeromonas hydrophila disease of ducks. Fortunately, the Chinese herbal medicines indicated dual-direction regulation of immunity and antibacterial on ducks, and are degradable easily as well as free from residues, and have no toxic side effects, which are deemed as ideal medicines for treating aeromonas hydrophila disease of ducks. Thus, it is urgently to develop a traditional Chinese medicine composition for treating aeromonas hydrophila disease of ducks by Scientific and technical staff of this field.

Summary of the Invention The purpose of the present invention is to overcome the existing shortcomings of the treatment methods for the Aeromonas hydrophila disease of ducks, and to provide a traditional Chinese medicine decoction to treat the Aeromonas hydrophila disease of ducks, which is easy to obtain, convenient to prepare, and can reduce drug residues and toxic and side effects, as well as demonstrate high curative effect at low cost. A traditional Chinese medicine composition for treating aeromonas hydrophila disease of ducks, which comprises components by weight as follows: 40-60 parts of Discolor Cinquefoil Herb, 40-60 parts of Rhizoma Paridis and 35-55 parts of Rabdosia Rubescens, 35-55 parts of Hibiscus, 30-50 parts of Pericarpium Granati, -50 parts of Polygonum Hydropiper L, 30-50 parts of Chinese Gall, 30-50 parts of Garden Burnet, 25-45 parts of Yerbadetajo Herb, 25-45 parts of Herba Agrimoniae, -45 parts of Radix Bupleuri , 20-40 parts of Fructus Chebulae, 20-40 parts of Fructus Mume, 20-40 parts of Cherokee Rose, 20-40 parts of Fructus Aristolochiae, -40 parts of Greater Celandine Herb, 15-35 parts of Flos Caryophyllata, 15-35 parts of Szechwan Chinaberry Fruit, and 15-35 parts of Rhizoma alismatis. The preferred components by weight comprise: 60 parts of Discolor Cinquefoil Herb, 60 parts of Rhizoma Paridis and 55 parts of Rabdosia Rubescens, 55 parts of Hibiscus, 50 parts of Pericarpium Granati, 50 parts of Polygonum Hydropiper L, 50 parts of Chinese Gall, 50 parts of Garden Burnet, 45 parts of Yerbadetajo Herb, 45 parts of Herba Agrimoniae, 45 parts of Radix Bupleuri , 40 parts of Fructus Chebulae, 40 parts of Fructus Mume, 40 parts of Cherokee Rose, 40 parts of Fructus Aristolochiae, 40 parts of Greater Celandine Herb, 35 parts of Flos Caryophyllata, 35 parts of Szechwan Chinaberry Fruit, 35 parts of Rhizoma alismatis. Or: 40 parts of Discolor Cinquefoil Herb, 40 parts of Rhizoma Paridis and 35 parts of Rabdosia Rubescens, 35 parts of Hibiscus, 30 parts of Pericarpium Granati, 30

Descriptions

parts of Polygonum Hydropiper L, 30 parts of Chinese Gall, 30 parts of Garden Burnet, 25 parts of Yerbadetajo Herb, 25 parts of Herba Agrimoniae, 25 parts of Radix Bupleuri , 20 parts of Fructus Chebulae, 20 parts of Fructus Mume, 20 parts of Cherokee Rose, 20 parts of Fructus Aristolochiae, 20 parts of Greater Celandine Herb, 15 parts of Flos Caryophyllata, 15 parts of Szechwan Chinaberry Fruit, 15 parts of Rhizoma alismatis. Or: 50 parts of Discolor Cinquefoil Herb, 50 parts of Rhizoma Paridis and 45 parts of Rabdosia Rubescens, 45 parts of Hibiscus, 40 parts of Pericarpium Granati, 40 parts of Polygonum Hydropiper L, 40 parts of Chinese Gall, 40 parts of Garden Bumet, 35 parts of Yerbadetajo Herb, 35 parts of Herba Agrimoniae, 35 parts of Radix Bupleuri , 30 parts of Fructus Chebulae, 30 parts of Fructus Mume, 30 parts of Cherokee Rose, 30 parts of Fructus Aristolochiae, 30 parts of Greater Celandine Herb, 25 parts of Flos Caryophyllata, 25 parts of Szechwan Chinaberry Fruit, 25 parts of Rhizoma alismatis. The purpose of the present invention is mainly to provide a traditional Chinese medicine composition for treating aeromonas hydrophila disease of ducks, which is prepared according to following method: 1. Weigh and take raw materials as per the ratios of the said traditional Chinese medicine composition for treating aeromonas hydrophila disease of ducks according to claim 1, then, mix the Discolor Cinquefoil Herb, the Rhizoma Paridis, the Pericarpium Granati, the Chinese Gall, the Garden Bumet, the Radix Bupleuri, the Fructus Chebulae, the Fructus Mume, the Cherokee Rose, the Fructus Aristolochiae and the Szechwan Chinaberry Fruit according to aforesaid ratios by weight, after that, put them into a pulverizer to pulverize them into fine powder, and then sieve it by a 120-mesh sieve; then, according to the cold-maceration method, put the pulverized medicine into an infusion kettle, then, add 70% alcohol, wherein, the volume of alcohol added shall be 3 cm high above the powder, after that, cover the lid for impregnating for 5 days, during this time, keep shaking or stirring frequently to accelerate leaching. After that, take out the impregnating solution, filter it, and squeeze the medicine residues, after that, combine the obtained squeezed liquid with the leaching solution. Then, put the medicine residues into the impregnator again, then, add 70% alcohol, after that, impregnate it for 5 days, and squeeze the medicine residues again, and then, combine the obtained squeezed liquid with the leaching solution. Finally, mix both soaking solution together, filter it again, and then concentrate it to remove the ethanol, and thus, a viscous liquid can be obtained, which is called as the No. 1 herb liquor, then, reserve it for standby application.

Descriptions

2. Weigh and take raw materials according to the ratios of the said traditional Chinese medicine composition for treating aeromonas hydrophila disease of ducks, then, mix the Rabdosia Rubescens, the Hibiscus, the Polygonum Hydropiper L, the Yerbadetajo Herb, the Herba Agrimoniae, the Greater Celandine Herb, the Flos Caryophyllata, and the Rhizoma alismatis evenly, and then, add the water 6 times as heavy as the materials for impregnating for 40 minutes. After that, put them in a decoction pot, and then decoct them for 30 minutes, after that, filter them to remove the medicine residues, and thus, the filtrate can be obtained. Then, put the medicine residues in the decoction pot again, and then decoct them for 30 minutes, after that, filter them to remove the medicine residues again, and thus, the filtrate can be obtained. Finally, combine both filtrates, and thus the No. 2 herb liquor can be obtained, then, reserve it for standby application. Mix the No. 1 and No. 2 herb liquors, and then, stir them evenly, and thus a traditional Chinese medicine composition for treating aeromonas hydrophila disease of ducks can be obtained. The composition principle of the present invention: The traditional veterinary science which circulates in China points out that the aeromonas hydrophila disease of ducks is caused by the epidemic toxin, thus, the treatment method shall have the effects of clearing heat and removing toxicity, cooling blood for hemostasis, and relieving diarrhea with astringents. For this prescription, the Discolor Cinquefoil Herb is sweet and bitter in taste and cold in nature, which is distributed in the liver, stomach and large intestinal meridians. In terms of aforesaid properties of this medicine, the sweet taste can reduce toxins, and the cold property can remove heat, as well as the bitter taste can eliminate dampness, and thus, this medicine has the efficacy of clearing heat and removing toxicity, hemostasis and relieving diarrhea, which can achieve better treatment effect on abdominal pain and diarrhea, and lingering dysentery especially. The Rhizoma Paridis is bitter in taste and slightly cold in nature, which is distributed in the liver meridian, and has the efficacy of clearing heat and removing toxicity, relieve swelling and pain. In addition, the Rabdosia Rubescens is bitter and sweet in taste and slightly cold in nature, which is distributed in the lung, kidney, and liver meridians, and has the efficacy of clearing heat and removing toxicity, and invigorating blood circulation and alleviating pain. The white Hibiscus is sweet and bitter in taste and cool in nature, which is distributed in the spleen, lung, and liver meridians, and has the efficacy of clearing heat and eliminating dampness, cooling blood and checking dysentery. Furthermore, the combination of these four medicines would achieve significant therapeutic effect of clearing heat and

Descriptions

removing toxicity, and have better effect on the treatment of aeromonas hydrophila disease of ducks, and thus, this medicine constitutes the sovereign medicine of the prescription. The Pericarpium Granati is warm in nature and acerb in taste, which is distributed in the large intestine, lung, and kidney meridians, and has the efficacy of relieving diarrhea with astringents, hemostasis and expelling parasite. Thus, this medicine can be used to treat chronic diarrhea, lingering dysentery, hematochezia, and rectal hernia, etc. The Polygonum Hydropiper L is warm in nature, which is distributed in the stomach and large intestine meridians, and has the efficacy of detoxication, eliminating dampness, dissipating blood stasis, and hemostasis. This medicine can achieve effective therapeutic effect on dysentery, gastroenteritis, and diarrhea, etc. The Chinese Gall is acerb in taste and cold in nature, which has the efficacy of astringing lung for decreasing internal heat, relieving diarrhea with astringents, hemostasis and astringing dampness. Furthermore, the Garden Burnet is bitter and acid in taste and slightly cold in nature, which is distributed in the liver, stomach and large intestine meridians, and has the efficacy of cooling blood for hemostasis, and astringing for detoxification. The Yerbadetajo Herb is cold in nature and sweet and sour in taste, which is distributed in the liver and kidney meridians, and has the efficacy of tonifying yin of the liver and kidney, and cooling blood for hemostasis. This medicine can achieve effective therapeutic effect on hemorrhage syndrome resulted from deficiency of Yin and blood heat especially, and has good efficacy of cooling blood, hemostasis, tonifying kidney, and nourishing yin, as well as can achieve effective therapeutic effect on various hemorrhagic diseases. The Herba Agrimoniae is bitter and astringent in taste, and neutral in nature, which is distributed in the stomach, liver, spleen, lung and large intestine meridians, and has the efficacy of astringency and hemostasis, checking dysentery and detoxification. Thus, the combination of these six medicines has significant efficacy of cooling blood for hemostasis, dissipating blood stasis and detoxification, as well as relieving diarrhea with astringents, so that the aforesaid six medicines constitute the ministerial medicine of the prescription together. In addition, the Herba Agrimoniae would have excellent efficacy of clearing heat and removing toxicity, and checking dysentery as well as eliminating foul secretions, if administered with white Hibiscus. Wherein, the Herba Agrimoniae is bitter and astringent in taste and has the efficacy of hemostasis with astringents; and the white Hibiscus is sweet in taste and cold in nature, which has the efficacy of clearing heat and removing toxicity, activating blood circulation and expelling pus, as well as cooling blood and checking dysentery, thus, both medicines can complement each other, and would have the effect of clearing heat and removing toxicity and limbering up if administrated by means of combination. Furthermore, such

Descriptions

combination also has the efficacy of clearing heat, cooling blood, hemostasis and stopping dysentery, as well as can enhance the function of detoxification and eliminating foul secretions, so that it has better therapeutic effect on treating aeromonas hydrophila disease of green ducks. The Radix Bupleuri is bitter in taste and slightly cold in nature, which is distributed in the liver, gallbladder, pericardium, and triple energizer meridians, and has the efficacy of reconciliation and abatement of fever, soothing the liver and regulating vital energy, as well as raising Yang Qi. Thus, this medicine constitutes the priority medicine for reconciling the Shaoyang Meridians, which has good properties and has the efficacy of catharsis, as well as can achieve good effect of soothing the liver and relieving depression, so that it can be served as the priority medicine for treating the stagnation of liver qi. Moreover, it has the advantage of raising the Qi of lucid yang, which is suitable for treating chronic diarrhea and rectal prolapse caused by collapse from qi deficiency. The Fructus Chebulae is bitter, sour and astringent in taste, and warm in nature, which is distributed in the lung and large intestine meridian, and has the efficacy of astringent and astringent intestines, as well as aerofluxus and checking diarrhea. The Fructus Mume is sour and astringent in taste and neutral in nature, which is distributed in the liver, spleen, lung, and large intestine meridians, and has the efficacy of astringing lung for relieving cough, relieving diarrhea with astringents, as well as engendering liquid and allaying thirst. Thus, this medicine can achieve significant therapeutic effect on lung deficiency and chronic cough, lingering dysentery and laxation, etc. The Cherokee Rose is sour, sweet and astringent in taste and neutral in nature, which is distributed in the kidney, bladder, and large intestine meridians, and has the efficacy of securing essence and reducing urination, curing metrorrhagia and leukorrhagia, as well as relieving diarrhea with astringents. The combination of the aforesaid four medicines can achieve significant therapeutic effect on relieving diarrhea with astringents. The Fructus Aristolochiae is bitter in taste, and slightly pungent and cold in nature, which is distributed in the lung and large intestine meridians, and has the efficacy of clearing lung and eliminating phlegm, relieving cough and asthma and clearing intestines and diminishing hemorrhoids. Thus, this medicine is often used for treating cough with lung heat, abundant expectoration and shortness of breath, etc., which also can achieve significant therapeutic effect on clearing heat and calming the liver, so that it can be used for treating hypertensive disease caused by hyperactivity of liver-yang. The Fructus Aristolochiae can be administrated with the Garden Burnet in this prescription, and the combination would have excellent efficacy of clearing intestines and hemostasis. The Greater Celandine Herb is cold in nature and bitter in taste, which is distributed in the lung,

Descriptions

heart, and kidney meridians, and has the efficacy of alleviating pain, relieving a cough, diuresis and detoxification, etc. Thus, this medicine is mostly used for treating stomach pain, abdominal pain, enteritis, dysentery, and cough, etc. In addition, the combination of the Fructus Aristolochiae and the Greater Celandine Herb has excellent efficacy of clearing lungs and depressing qi, relieving cough and asthma, as well as clearing intestines and diuresis. Furthermore, the Flos Caryophyllata is pungent in taste and warm in nature, which is distributed in the lung, stomach, spleen, and kidney meridians, and has the efficacy of warming middle energizer for descending adverse qi, warming the kidney and supporting yang. Thus, this medicine constitutes the priority medicine for treating gastric cold and hiccough, which has the efficacy of warming the kidney and tonifying Yang, so that it can be used for treating diarrhea, etc. The Szechwan Chinaberry Fruit is bitter in taste and cold in nature, which is distributed in the liver and stomach meridians, and has the efficacy of promoting the circulation of qi to relieve pain and getting rid of intestinal parasites. Thus, this medicine can achieve significant therapeutic effect on abdominal and rib-side pain, colic, and abdominal pain due parasitic infestation, etc. The Rhizoma Alismatis is sweet and bland in taste and cold in nature, which is distributed in the kidney and bladder meridians, and has the efficacy of inducing diuresis and excreting dampness, and discharging kidney fire. Thus, this medicine can achieve significant therapeutic effect on difficult urination caused by internal stagnation of fluid-dampness, edema and tumescence, damp heat and stranguria with turbid discharge, as well as chromic diarrhea. The aforesaid nine medicines constitute the envoy medicines in the prescription together, and the combination of all medicines has the efficacy of clearing away heat and removing toxicity, relieving diarrhea with astringents, and cooling blood for hemostasis, and thus it can achieve significant therapeutic effect on aeromonas hydrophila disease of ducks. The characteristics of the prescription include that: this prescription can be administrated for the purpose of clearing heat and removing toxicity, relieving diarrhea with astringents, and cooling blood for hemostasis. Meanwhile, it has the efficacy of regulating the activity of Qi and astringing lung for relieving cough, nourishing liver and kidney and inducing diuresis and excreting dampness. The aforesaid medicines are organically combined, administered dialectically and compatible as well as can be mutually adjuvant, and thus they can enhance the efficacy of clearing heat and removing toxicity, relieving diarrhea with astringents, and cooling blood for hemostasis, and further achieve the purpose of treating the aeromonas hydrophila disease of ducks. In addition, some clinic-controlled trials have demonstrated that the cure rate of this prescription for

Descriptions

treating the aeromonas hydrophila disease of ducks reached 94.20%, while the effective rate was 97.81%.

Detailed Description of the Presently Preferred Embodiments Embodiment 1 The components by weight comprise: 60 parts of Discolor Cinquefoil Herb, parts of Rhizoma Paridis and 55 parts of Rabdosia Rubescens, 55 parts of Hibiscus, 50 parts of Pericarpium Granati, 50 parts of Polygonum Hydropiper L, parts of Chinese Gall, 50 parts of Garden Burnet, 45 parts of Yerbadetajo Herb, parts of Herba Agrimoniae, 45 parts of Radix Bupleuri , 40 parts of Fructus Chebulae, 40 parts of Fructus Mume, 40 parts of Cherokee Rose, 40 parts of Fructus Aristolochiae, 40 parts of Greater Celandine Herb, 35 parts of Flos Caryophyllata, 35 parts of Szechwan Chinaberry Fruit, 35 parts of Rhizoma alismatis. Embodiment 2 The components by weight comprise: 40 parts of Discolor Cinquefoil Herb, parts of Rhizoma Paridis and 35 parts of Rabdosia Rubescens, 35 parts of Hibiscus, 30 parts of Pericarpium Granati, 30 parts of Polygonum Hydropiper L, parts of Chinese Gall, 30 parts of Garden Burnet, 25 parts of Yerbadetajo Herb, parts of Herba Agrimoniae, 25 parts of Radix Bupleuri , 20 parts of Fructus Chebulae, 20 parts of Fructus Mume, 20 parts of Cherokee Rose, 20 parts of Fructus Aristolochiae, 20 parts of Greater Celandine Herb, 15 parts of Flos Caryophyllata, 15 parts of Szechwan Chinaberry Fruit, 15 parts of Rhizoma alismatis. Embodiment 3 The components by weight comprise: 50 parts of Discolor Cinquefoil Herb, parts of Rhizoma Paridis and 45 parts of Rabdosia Rubescens, 45 parts of Hibiscus, 40 parts of Pericarpium Granati, 40 parts of Polygonum Hydropiper L, parts of Chinese Gall, 40 parts of Garden Bumet, 35 parts of Yerbadetajo Herb, parts of Herba Agrimoniae, 35 parts of Radix Bupleuri , 30 parts of Fructus Chebulae, 30 parts of Fructus Mume, 30 parts of Cherokee Rose, 30 parts of Fructus Aristolochiae, 30 parts of Greater Celandine Herb, 25 parts of Flos Caryophyllata, 25 parts of Szechwan Chinaberry Fruit, 25 parts of Rhizoma alismatis. Embodiment 4 According to the proportions of embodiment 1, 2 or 3, a medicine composition for treating the Monday morning fever shall be prepared through following method: 1. Weigh and take raw materials as per the ratios of the said traditional

Descriptions

Chinese medicine composition for treating aeromonas hydrophila disease of ducks according to claim 1, then, mix the Discolor Cinquefoil Herb, the Rhizoma Paridis, the Pericarpium Granati, the Chinese Gall, the Garden Bumet, the Radix Bupleuri, the Fructus Chebulae, the Fructus Mume, the Cherokee Rose, the Fructus Aristolochiae and the Szechwan Chinaberry Fruit according to aforesaid ratios by weight, after that, put them into a pulverizer to pulverize them into fine powder, and then sieve it by a 120-mesh sieve; then, according to the cold-maceration method, put the pulverized medicine into an infusion kettle, then, add 70% alcohol, wherein, the volume of alcohol added shall be 3 cm high above the powder, after that, cover the lid for impregnating for 5 days, during this time, keep shaking or stirring frequently to accelerate leaching. After that, take out the impregnating solution, filter it, and squeeze the medicine residues, after that, combine the obtained squeezed liquid with the leaching solution. Then, put the medicine residues into the impregnator again, then, add 70% alcohol, after that, impregnate it for 5 days, and squeeze the medicine residues again, and then, combine the obtained squeezed liquid with the leaching solution. Finally, mix both soaking solution together, filter it again, and then concentrate it to remove the ethanol, and thus, a viscous liquid can be obtained, which is called as the No. 1 herb liquor, then, reserve it for standby application. 2. Weigh and take raw materials according to the ratios of the said traditional Chinese medicine composition for treating aeromonas hydrophila disease of ducks, then, mix the Rabdosia Rubescens, the Hibiscus, the Polygonum Hydropiper L, the Yerbadetajo Herb, the Herba Agrimoniae, the Greater Celandine Herb, the Flos Caryophyllata, and the Rhizoma alismatis evenly, and then, add the water 6 times as heavy as the materials for impregnating for 40 minutes. After that, put them in a decoction pot, and then decoct them for 30 minutes, after that, filter them to remove the medicine residues, and thus, the filtrate can be obtained. Then, put the medicine residues in the decoction pot again, and then decoct them for 30 minutes, after that, filter them to remove the medicine residues again, and thus, the filtrate can be obtained. Finally, combine both filtrates, and thus the No. 2 herb liquor can be obtained, then, reserve it for standby application. Mix the No. 1 and No. 2 herb liquors, and then, stir them evenly, and thus a traditional Chinese medicine composition for treating aeromonas hydrophila disease of ducks can be obtained. Wherein, the traditional Chinese medicine composition for treating aeromonas hydrophila disease of ducks prepared in embodiment 1 and embodiment 4 contains 1.2 grams of crude medicine per milliliter; The traditional Chinese medicine composition for treating aeromonas

Descriptions

hydrophila disease of ducks prepared in embodiment 2 and embodiment 4 contains 1.0 grams of crude medicine per milliliter; The traditional Chinese medicine composition for treating aeromonas hydrophila disease of ducks prepared in embodiment 3 and embodiment 4 contains 1.1 grams of crude medicine per milliliter Experimental Example 1. Method: selected six duck farms and self-employed duck farmers where the aeromonas hydrophila disease of ducks occurred, and selected 4,673 diseased ducklings in total, whose ages are between 10-day old and 28-day old. Then, divide the diseased ducklings into three groups, i.e., the group of the present invention, the group administered the prescription composed of Cortex Phellodendri, Scutellaria, Rhizoma Coptidis and Radix Pulsatillae, and the control group. The group of the present invention: drenched 4 ml of the concentrated medicinal solution prepared by the embodiment 3 and the embodiment 4 twice to each duckling. For the diseased ducklings which can drink water, add 30% brown sugar to the medicinal solution for easy drinking. In addition, for those suffered from the disease seriously, use a dropper to administrate 15 drops of medicinal solution twice a day to each duckling. In terms of controlling the temperature of medicine: administrate them warm at the temperature between 40°C and 50°C. The group administered the prescription composed of Cortex Phellodendri, Scutellaria, Rhizoma Coptidis and Radix Pulsatillae: the prescription comprised 500g of Cortex Phellodendri, Scutellaria, and Radix Pulsatillae, respectively, and 300g of Rhizoma Coptidis, Polyporus, Rhizoma Alismatis, respectively, as well as 200g of Fructus Aurantii, Magnolia Officinalis, Fructus Amomi and Rhizoma Atractylodis, respectively. The administration method was: decocted the medicines with water twice, then, combined the medicinal solution, after that, added 30% brown sugar for drinking by ducks, and one dose per day should be administrated for 5 days continuously. Furthermore, in terms of controlling the temperature of medicine: administrated them warm at the temperature between 40°C and 50°C. The aforesaid amount is equivalent to the daily dosage for 1,000 ducklings. The control group: only added appropriate amount of sugar to the drinking water. In terms of controlling the water temperature: administrated them warm at the temperature between 40°C and 50°C. The treatment time should be five days, and the treatment or administration by drinking should be implemented once in the morning and afternoon every day; Table 1 Groups of ducklings and the main treatment methods Group Number of Diseased Treatment Methods Ducklings

Descriptions

The group of the present 1828 Add 30% brown sugar to the medicinal invention solution for drinking by ducklings, or drenched 4 ml of the medicinal solution to each gosling a day The group administered the 1584 clyster once every morning and prescription composed of afternoon, and dosage measures Cortex Phellodendri, according to 4ml/kg of the body weight Scutellaria, Rhizoma Coptidis and Radix Pulsatillae The control group 1261 Add 30% brown sugar to water for drinking by ducklings

2. Determination of therapeutic effect: Cure: Relevant symptoms of aeromonas hydrophila disease of ducks disappeared completely, and the appetite and drinking behavior of ducks returned to normal, as well as there were no abnormalities detected through pathological autopsy. Improved: Relevant symptoms of aeromonas hydrophila disease of ducks have improved, and the appetite and drinking behavior of ducks have recovered to some extent, and the results of pathological autopsy got better. Invalid: Relevant symptoms of aeromonas hydrophila disease of ducks remained unchanged, and the appetite and drinking behavior of ducks as well as the results of pathological autopsy were the same as those prior to treatment. 3. Results: 4673 ducklings were treated with different dosing schemes and after five days of treatment, the treatment effects of each group are shown in Table 2; Table 2 Treatment effects of ducklings by means of various treatment schemes Group Number of Number of Number of Number of Cure rate Total ducklings ducklings ducklings ducklings effective received cured improved manifested rate treatment no effect The group of 1828 1722 66 40 94.2 97.81 the present 0 invention The group 1584 1287 137 160 81.2 89.90 administered 5 the prescription

Descriptions

composed of Cortex Phellodendri, Scutellaria, Rhizoma Coptidis and Radix Pulsatillae The 1261 212 346 703 16.8 44.25 control group

It can be seen from Table 2 that the therapeutic effect on aeromonas hydrophila disease of ducks to the treatment group and the control group were different. Wherein, the therapeutic effect to the group of the present invention was the best, and the cure rate of which reached up to 94.20% and the total effective rate reached up to 97.81%, which was extremely higher than that of other two groups (P <0.01); and the group administered the prescription composed of Cortex Phellodendri, Scutellaria, Rhizoma Coptidis and Radix Pulsatillae followed, meanwhile the control group manifested no therapeutic effect. The statistical results indicated that the differences among the groups were extremely significant (P<0.01). The 40 ducklings of the group of the present invention which manifested no therapeutic effect were all cases that died in the early treatment (i.e., 1-4 days). These ducks died for failing to wait for the efficacy of medicines to come into effect fully, due to suffering from serious disease and multiple system organ failure in vivo. The practice has proved that the medicine of the present invention not only has the efficacy of clearing heat and removing toxicity, relieving diarrhea with astringents, and cooling blood for hemostasis, but also can regulate the activity of Qi and astringe lung for relieving cough, nourish liver and kidney and induce diuresis and excrete dampness, and thus, it can effectively treat the aeromonas hydrophila disease of ducks, also can achieve significant effect of sterilization, detoxification and improving the immune function of the body, and further show significant therapeutic effect. This medicine provides a practical and feasible new medicine for clinical treatment of the disease due to the abundant resources, low cost, and high cure rate, as well as having no residues, free from toxic side effects, and is easy to be absorbed by ducklings. The aforesaid reference embodiment describes the traditional Chinese medicine composition for treating aeromonas hydrophila disease of ducks and its

Descriptions

preparation method in detail, which is illustrative rather than restrictive. Several embodiments can be listed according to the limited scope, and thus any variations and modifications completed without departing from the general idea of the present invention should fall within the protection scope of the present invention.

Claims

1. A traditional Chinese medicine composition for treating aeromonas hydrophila disease of ducks, which comprises components by weight as follows: 40-60 parts of Discolor Cinquefoil Herb, 40-60 parts of Rhizoma Paridis and 35-55 parts of Rabdosia Rubescens, 35-55 parts of Hibiscus, 30-50 parts of Pericarpium Granati, -50 parts of Polygonum Hydropiper L, 30-50 parts of Chinese Gall, 30-50 parts of Garden Burnet, 25-45 parts of Yerbadetajo Herb, 25-45 parts of Herba Agrimoniae, -45 parts of Radix Bupleuri , 20-40 parts of Fructus Chebulae, 20-40 parts of Fructus Mume, 20-40 parts of Cherokee Rose, 20-40 parts of Fructus Aristolochiae, -40 parts of Greater Celandine Herb, 15-35 parts of Flos Caryophyllata, 15-35 parts of Szechwan Chinaberry Fruit, and 15-35 parts of Rhizoma alismatis.

2. The said traditional Chinese medicine composition for treating aeromonas hydrophila disease of ducks according to claim 1, characterized in that comprising components by weight as follows: 60 parts of Discolor Cinquefoil Herb, 60 parts of Rhizoma Paridis and 55 parts of Rabdosia Rubescens, 55 parts of Hibiscus, 50 parts of Pericarpium Granati, 50 parts of Polygonum Hydropiper L, 50 parts of Chinese Gall, 50 parts of Garden Burnet, 45 parts of Yerbadetajo Herb, 45 parts of Herba Agrimoniae, 45 parts of Radix Bupleuri , 40 parts of Fructus Chebulae, 40 parts of Fructus Mume, 40 parts of Cherokee Rose, 40 parts of Fructus Aristolochiae, 40 parts of Greater Celandine Herb, 35 parts of Flos Caryophyllata, 35 parts of Szechwan Chinaberry Fruit, 35 parts of Rhizoma alismatis.

3. The said traditional Chinese medicine composition for treating aeromonas hydrophila disease of ducks according to claim 1, characterized in that comprising components by weight as follows: 40 parts of Discolor Cinquefoil Herb, 40 parts of Rhizoma Paridis and 35 parts of Rabdosia Rubescens, 35 parts of Hibiscus, 30 parts of Pericarpium Granati, 30 parts of Polygonum Hydropiper L, 30 parts of Chinese Gall, 30 parts of Garden Burnet, 25 parts of Yerbadetajo Herb, 25 parts of Herba Agrimoniae, 25 parts of Radix Bupleuri , 20 parts of Fructus Chebulae, 20 parts of Fructus Mume, 20 parts of Cherokee Rose, 20 parts of Fructus Aristolochiae, 20 parts of Greater Celandine Herb, 15 parts of Flos Caryophyllata, 15 parts of Szechwan Chinaberry Fruit, 15 parts of Rhizoma alismatis.

4. The said traditional Chinese medicine composition for treating aeromonas hydrophila disease of ducks according to claim 1, characterized in that comprising components by weight as follows: 50 parts of Discolor Cinquefoil Herb, 50 parts of Rhizoma Paridis and 45 parts of Rabdosia Rubescens, 45 parts of Hibiscus, 40 parts of Pericarpium Granati, 40 parts of Polygonum Hydropiper L, 40 parts of Chinese Gall, 40 parts of Garden Burnet, 35 parts of Yerbadetajo Herb, 35 parts of Herba Agrimoniae, 35 parts of Radix Bupleuri , 30 parts of Fructus Chebulae, 30

Claims parts of Fructus Mume, 30 parts of Cherokee Rose, 30 parts of Fructus Aristolochiae, 30 parts of Greater Celandine Herb, 25 parts of Flos Caryophyllata, 25 parts of Szechwan Chinaberry Fruit, 25 parts of Rhizoma alismatis.

5. A traditional Chinese medicine composition for treating aeromonas hydrophila disease of ducks, which is prepared according to following method: 1) Weigh and take raw materials as per the ratios of the said traditional Chinese medicine composition for treating aeromonas hydrophila disease of ducks according to claim 1, then, mix the Discolor Cinquefoil Herb, the Rhizoma Paridis, the Pericarpium Granati, the Chinese Gall, the Garden Bumet, the Radix Bupleuri, the Fructus Chebulae, the Fructus Mume, the Cherokee Rose, the Fructus Aristolochiae and the Szechwan Chinaberry Fruit according to aforesaid ratios by weight, after that, put them into a pulverizer to pulverize them into fine powder, and then sieve it by a 120-mesh sieve; According to the cold-maceration method, put the pulverized medicine into an infusion kettle, then, add 70% alcohol, wherein, the volume of alcohol added shall be 3 cm high above the powder, after that, cover the lid for impregnating for 5 days, during this time, keep shaking or stirring frequently to accelerate leaching; Then, take out the impregnating solution, filter it, and squeeze the medicine residues, after that, combine the obtained squeezed liquid with the leaching solution; Put the medicine residues into the impregnator again, then, add 70% alcohol, after that, impregnate it for 5 days, and squeeze the medicine residues again, then, combine the obtained squeezed liquid with the leaching solution; Mix both soaking solution together, filter it again, and then concentrate it to remove the ethanol, and thus, a viscous liquid can be obtained, which is called as the No. 1 herb liquor, then, reserve it for standby application; 2) Weigh and take raw materials as per the ratios of the said traditional Chinese medicine composition for treating aeromonas hydrophila disease of ducks according to claim 1, then, mix the Rabdosia Rubescens, the Hibiscus, the Polygonum Hydropiper L, the Yerbadetajo Herb, the Herba Agrimoniae, the Greater Celandine Herb, the Flos Caryophyllata, and the Rhizoma alismatis evenly, and then, add the water 6 times as heavy as the materials for impregnating for 40 minutes; Put them in a decoction pot, and then decoct them for 30 minutes, after that, filter them to remove the medicine residues, and thus, the filtrate can be obtained; Put the medicine residues in the decoction pot again, and then decoct them for minutes, after that, filter them to remove the medicine residues again, and thus, the filtrate can be obtained; Combine both filtrates, and thus the No. 2 herb liquor can be obtained, then,

Claims reserve it for standby application; Mix the No. 1 and No. 2 herb liquors, and then, stir them evenly, and thus a traditional Chinese medicine composition for treating aeromonas hydrophila disease of ducks can be obtained.