AU2021105657A4 - Biochar-based probiotic preparation and preparation method thereof - Google Patents
Biochar-based probiotic preparation and preparation method thereof Download PDFInfo
- Publication number
- AU2021105657A4 AU2021105657A4 AU2021105657A AU2021105657A AU2021105657A4 AU 2021105657 A4 AU2021105657 A4 AU 2021105657A4 AU 2021105657 A AU2021105657 A AU 2021105657A AU 2021105657 A AU2021105657 A AU 2021105657A AU 2021105657 A4 AU2021105657 A4 AU 2021105657A4
- Authority
- AU
- Australia
- Prior art keywords
- biochar
- pellet
- nutrient particles
- probiotic
- particles
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 239000006041 probiotic Substances 0.000 title claims abstract description 60
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 60
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 60
- 238000002360 preparation method Methods 0.000 title claims abstract description 38
- 239000002245 particle Substances 0.000 claims abstract description 83
- 235000015097 nutrients Nutrition 0.000 claims abstract description 59
- 239000008188 pellet Substances 0.000 claims abstract description 54
- 239000007787 solid Substances 0.000 claims description 27
- 239000000835 fiber Substances 0.000 claims description 22
- 239000000017 hydrogel Substances 0.000 claims description 22
- 239000011859 microparticle Substances 0.000 claims description 22
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 18
- 241000233866 Fungi Species 0.000 claims description 18
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 15
- 229920002261 Corn starch Polymers 0.000 claims description 15
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical class OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 15
- 244000068988 Glycine max Species 0.000 claims description 15
- 235000010469 Glycine max Nutrition 0.000 claims description 15
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 15
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 15
- 239000011248 coating agent Substances 0.000 claims description 15
- 238000000576 coating method Methods 0.000 claims description 15
- 239000008120 corn starch Substances 0.000 claims description 15
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 15
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 15
- 239000002154 agricultural waste Substances 0.000 claims description 12
- 238000002156 mixing Methods 0.000 claims description 12
- 241000894006 Bacteria Species 0.000 claims description 11
- 238000005507 spraying Methods 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 229910052757 nitrogen Inorganic materials 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 8
- 239000002952 polymeric resin Substances 0.000 claims description 8
- 238000004880 explosion Methods 0.000 claims description 7
- 239000008187 granular material Substances 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 5
- 238000000227 grinding Methods 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- 229920000247 superabsorbent polymer Polymers 0.000 claims 1
- 230000035899 viability Effects 0.000 abstract description 4
- 238000001179 sorption measurement Methods 0.000 abstract description 3
- 239000002689 soil Substances 0.000 description 12
- 239000002250 absorbent Substances 0.000 description 7
- 229920003002 synthetic resin Polymers 0.000 description 7
- 244000005700 microbiome Species 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 2
- 240000000599 Lentinula edodes Species 0.000 description 2
- 235000001715 Lentinula edodes Nutrition 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 230000035558 fertility Effects 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241001313734 Dictyophora Species 0.000 description 1
- 241001313710 Dictyophora indusiata Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 235000010980 cellulose Nutrition 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000003864 humus Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 229920000058 polyacrylate Polymers 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000002786 root growth Effects 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
- C05G5/14—Tablets, spikes, rods, blocks or balls
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Soil Sciences (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Fertilizers (AREA)
Abstract
OF THE DISCLOSURE
The present disclosure discloses a biochar-based probiotic preparation and a
preparation method thereof. The biochar-based probiotic preparation comprises a
biochar pellet, nutrient particles embedded and internally attached to the biochar pellet,
and a probiotic flora attached to surfaces of the nutrient particles and between inner
walls of the nutrient particles and the biochar pellet. The biochar pellet obtained by the
present disclosure has loose and porous structure, large specific surface area, and strong
adsorption and chelating capacity, to absorb a large amount of the probiotic flora and
ensure the viability of the probiotic flora.
Description
[01] The present disclosure relates to the biochar preparation, specifically relates to a biochar-based probiotic preparation and a preparation method thereof.
[02] Biochar has developed porous structure, huge specific surface area and large adsorption capacity. It can improve the soil environment and its physico-chemical
properties, having a wide application prospect in the agricultural environment. This is
not only in line with the new concept of the low-carbon recycling and sustainable
agricultural development in China, but also can save energy, improve the environment
and ecological balance, which is especially of great significance for the straw recycling
and sustainable development in the planting industry.
[03] The soil microorganisms directly participate in the transformation of matter and
energy, the formation and decomposition of humus, the release of nutrients, and the
formation and development of soil fertility, such as nitrogen fixation in the soil.
Therefore, increasing the number of beneficial microorganisms in the soil can
strengthen the viability of the soil microorganisms, thereby improving the soil fertility.
However, the existing carriers of the soil microorganisms have low adhesion to
probiotic and low probiotic viability.
[04] To solve the above problems, the present disclosure provides a biochar-based
probiotic preparation and a preparation method thereof.
[05] To achieve the above objects, the technical solutions employed by the present
disclosure are as follows:
[06] The biochar-based probiotic preparation is provided, including a biochar pellet,
nutrient particles embedded and internally attached to the biochar pellet, and a probiotic flora attached to surfaces of the nutrient particles and between inner walls of the nutrient particles and the biochar pellet.
[07] Further, the biochar pellet is prepared by the following steps: crushing an agricultural waste by steam explosion, then pyrolyzing at 650-750°C for 2-3 h under the protection of nitrogen, and then mixing with an appropriate amount of solid hydrogel microparticles to granulate, to obtain the biochar pellet.
[08] Further, a particle size of the solid hydrogel microparticles is 0.7-1 [m, and a mass ratio of the solid hydrogel microparticles to the agricultural waste is 10:1.
[09] Further, the solid hydrogel microparticles are solid particles in a corresponding shape formed after a super-absorbent polymer resin in a certain shape fully absorbs water to expand.
[10] Further, a particle size of the nutrient particles is less than 0.5 [m, and a mass ratio of the nutrient particles to the biochar pellet is 1:5. And the nutrient particles are prepared from fructo-oligosaccharides, soybean peptides with small molecular weight, trehalose, edible fungus fiber, and nanoscale corn starch.
[11] Further, a mass ratio of the fructo-oligosaccharides, the soybean peptides with small molecular weight, trehalose, the edible fungus fiber, and the nanoscale corn starch is 1:0.3:0.2:5:2.
[12] Further, an amount of effective and viable bacteria of the probiotic flora is > 2 X 10 10/g, and an amount of effective and viable bacteria of the biochar-based probiotic preparation is > 2 X 10 9/g.
[13] The present disclosure provides the preparation method of the biochar-based probiotic preparation, including the following steps: Si. crushing the agricultural waste by steam explosion, then pyrolyzing at 650 750°C for 2-3 h under the protection of nitrogen, and then mixing with an appropriate amount of the solid hydrogel microparticles to granulate, to obtain the biochar pellet with a particle size of 24-30 mesh; S2. evenly mixing the fructo-oligosaccharides, the soybean peptides with small molecular weight, trehalose, the edible fungus fiber, and the nanoscale corn starch in the mass ratio of 1:0.3:0.2:5:2, then grinding, drying, and crushing, to obtain the nutrient particles with the particle size of less than 0.5 [m;
S3. putting the biochar pellet in a trundling pan of a centrifugal granulator, and
putting a paste of the nutrient particles (made by 1 part of the nutrient particles with 5
times an amount of water) in a feed chamber of a granulator equipped with a coating
system, then turning on the granulators to prepare the biochar pellet embedded and
internally attached with the nutrient particles by a powder layering method, for standby;
S4. completing an expanded culture of the probiotic flora to obtain a probiotic
solution; and
S5. putting the biochar pellet embedded and internally attached with the nutrient
particles in a fluidized bed coater for bottom spray coating with a liquid-spraying speed
of 5.0-6.0 mL/min and an air inlet frequency of 35-45 Hz, and using the probiotic
solution as a coating solution. A temperature in the fluidized bed coater is maintained
at 30°C, and the coated biochar pellet is dried in the fluidized bed coater after the
coating is completed, to obtain the biochar-based probiotic preparation.
[14] The beneficial effects of the present disclosure are as follows:
[15] The obtained biochar pellet has loose and porous structure, large specific
surface area, and strong adsorption and chelating capacity, to absorb a large amount of
the probiotic flora, and ensure the viability of the probiotic flora.
[16] The present disclosure will be further described in detail below in conjunction with specific examples. The following examples are used to help those skilled in the art
to understand the present disclosure better, not limiting the present disclosure in any
form. It shall be pointed out that various modifications and improvements could be
made by those ordinarily skilled in the art without deviating from the concept of the
present disclosure, which shall fall within the protection scope of the present disclosure.
[17] Example 1
[18] A biochar-based probiotic preparation is provided, including the biochar pellet,
the nutrient particles embedded and internally attached to the biochar pellet, and the
probiotic flora attached to the surfaces of the nutrient particles and between the inner walls of the nutrient particles and the biochar pellet. The biochar-based probiotic preparation is prepared by the following method: SI. crushing the agricultural waste by steam explosion, then pyrolyzing at 650 750°C for 2-3 h under the protection of nitrogen, and then mixing with an appropriate amount of the solid hydrogel microparticles to granulate, to obtain the biochar pellet with the particle size of 24-30 mesh; S2. evenly mixing the fructo-oligosaccharides, the soybean peptides with small molecular weight, trehalose, the edible fungus fiber, and the nanoscale corn starch in the mass ratio of 1:0.3:0.2:5:2, then grinding, drying, and crushing, to obtain the nutrient particles with the particle size of less than 0.5 [m; S3. putting the biochar pellet in the trundling pan of a centrifugal granulator, and putting the paste of the nutrient particles (made by 1 part of the nutrient particles with times an amount of water) in the feed chamber of a granulator equipped with a coating system, then turning on the granulators to prepare the biochar pellet embedded and internally attached with the nutrient particles by the powder layering method, for standby; S4. completing the expanded culture of the probiotic flora to obtain the probiotic solution; and S5. putting the biochar pellet embedded and internally attached with the nutrient particles in a fluidized bed coater for bottom spray coating with the liquid-spraying speed of5.0-6.0 mL/min andthe air inlet frequency of 35-45 Hz, andusing theprobiotic solution as the coating solution. The temperature in the fluidized bed coater is maintained at 30°C, and the coated biochar pellet is dried in the fluidized bed coater after the coating is completed, to obtain the biochar-based probiotic preparation.
[19] In this example, the particle size of the solid hydrogel microparticles is 0.7-1
[tm, and the mass ratio of the solid hydrogel microparticles to the agricultural waste is :1.
[20] In this example, the solid hydrogel microparticles are solid particles in a corresponding shape formed after the super-absorbent polymer resin in a certain shape fully absorbs water to expand. And the super-absorbent polymer resin is polyacrylates.
[21] In this example, the particle size of the nutrient particles is less than 0.5 rm, and the mass ratio of the nutrient particles to the biochar pellet is 1:5. The nutrient
particles are prepared from the fructo-oligosaccharides, the soybean peptides with small
molecular weight, trehalose, the edible fungus fiber, and the nanoscale corn starch. And
the edible fungus fiber is the fiber of Lentinus edodes.
[22] In this example, the mass ratio of the fructo-oligosaccharides, the soybean peptides with small molecular weight, trehalose, the edible fungus fiber, and the
nanoscale corn starch is 1:0.3:0.2:5:2.
[23] In this example, the amount of effective and viable bacteria of the probiotic flora is > 2X 10/g, and the amount of effective and viable bacteria of the biochar
based probiotic preparation is > 2 X 10 9/g.
[24] Example 2
[25] A biochar-based probiotic preparation is provided, including the biochar pellet, the nutrient particles embedded and internally attached to the biochar pellet, and the
probiotic flora attached to the surfaces of the nutrient particles and between the inner
walls of the nutrient particles and the biochar pellet. The biochar-based probiotic
preparation is prepared by the following method:
Si. crushing the agricultural waste by steam explosion, then pyrolyzing at 650
750°C for 2-3 h under the protection of nitrogen, and then mixing with an appropriate
amount of the solid hydrogel microparticles to granulate, to obtain the biochar pellet
with the particle size of 24-30 mesh;
S2. evenly mixing the fructo-oligosaccharides, the soybean peptides with small
molecular weight, trehalose, the edible fungus fiber, and the nanoscale corn starch in the
mass ratio of 1:0.3:0.2:5:2, then grinding, drying, and crushing, to obtain the nutrient
particles with the particle size of less than 0.5 m;
S3. putting the biochar pellet in the trundling pan of a centrifugal granulator, and
putting the paste of the nutrient particles (made by 1 part of the nutrient particles with
times an amount of water) in the feed chamber of a granulator equipped with a coating
system, then turning on the granulators to prepare the biochar pellet embedded and
internally attached with the nutrient particles by the powder layering method, for standby; S4. completing the expanded culture of the probiotic flora to obtain the probiotic solution; and S5. putting the biochar pellet embedded and internally attached with the nutrient particles in a fluidized bed coater for bottom spray coating with the liquid-spraying speed of5.0-6.0 mL/min and the air inlet frequency of 35-45 Hz, and using the probiotic solution as the coating solution. The temperature in the fluidized bed coater is maintained at 30°C, and the coated biochar pellet is dried in the fluidized bed coater after the coating is completed, to obtain the biochar-based probiotic preparation.
[26] In this example, the particle size of the solid hydrogel microparticles is 0.7-1
[im, and the mass ratio of the solid hydrogel microparticles to the agricultural waste is :1.
[27] In this example, the solid hydrogel microparticles are solid particles in a corresponding shape formed after the super-absorbent polymer resin in a certain shape fully absorbs water to expand. And the super-absorbent polymer resin is celluloses.
[28] In this example, the particle size of the nutrient particles is less than 0.5 [m, and the mass ratio of the nutrient particles to the biochar pellet is 1:5. The nutrient particles are prepared from the fructo-oligosaccharides, the soybean peptides with small molecular weight, trehalose, the edible fungus fiber, and the nanoscale corn starch. And the edible fungus fiber is the fiber of Dictyophora indusiata.
[29] In this example, the mass ratio of the fructo-oligosaccharides, the soybean peptides with small molecular weight, trehalose, the edible fungus fiber, and the nanoscale corn starch is 1:0.3:0.2:5:2.
[30] In this example, the amount of effective and viable bacteria of the probiotic flora is > 2X 10/g, and the amount of effective and viable bacteria of the biochar based probiotic preparation is > 2 X 10 9/g.
[31] Example 3
[32] A biochar-based probiotic preparation is provided, including the biochar pellet, the nutrient particles embedded and internally attached to the biochar pellet, and the probiotic flora attached to the surfaces of the nutrient particles and between the inner walls of the nutrient particles and the biochar pellet. The biochar-based probiotic preparation is prepared by the following method: SI. crushing the agricultural waste by steam explosion, then pyrolyzing at 650 750°C for 2-3 h under the protection of nitrogen, and then mixing with an appropriate amount of the solid hydrogel microparticles to granulate, to obtain the biochar pellet with the particle size of 24-30 mesh; S2. evenly mixing the fructo-oligosaccharides, the soybean peptides with small molecular weight, trehalose, the edible fungus fiber, and the nanoscale corn starch in the mass ratio of 1:0.3:0.2:5:2, then grinding, drying, and crushing, to obtain the nutrient particles with the particle size of less than 0.5 [m; S3. putting the biochar pellet in the trundling pan of a centrifugal granulator, and putting the paste of the nutrient particles (made by 1 part of the nutrient particles with times an amount of water) in the feed chamber of a granulator equipped with a coating system, then turning on the granulators to prepare the biochar pellet embedded and internally attached with the nutrient particles by the powder layering method, for standby; S4. completing the expanded culture of the probiotic flora to obtain the probiotic solution; and S5. putting the biochar pellet embedded and internally attached with the nutrient particles in a fluidized bed coater for bottom spray coating with the liquid-spraying speed of5.0-6.0 mL/min andthe air inlet frequency of 35-45 Hz, andusing theprobiotic solution as the coating solution. The temperature in the fluidized bed coater is maintained at 30°C, and the coated biochar pellet is dried in the fluidized bed coater after the coating is completed, to obtain the biochar-based probiotic preparation.
[33] In this example, the particle size of the solid hydrogel microparticles is 0.7-1
[tm, and the mass ratio of the solid hydrogel microparticles to the agricultural waste is :1.
[34] In this example, the solid hydrogel microparticles are solid particles in a corresponding shape formed after the super-absorbent polymer resin in a certain shape fully absorbs water to expand. And the super-absorbent polymer resin is proteins.
[35] In this example, the particle size of the nutrient particles is less than 0.5 [m, and the mass ratio of the nutrient particles to the biochar pellet is 1:5. The nutrient
particles are prepared from the fructo-oligosaccharides, the soybean peptides with small
molecular weight, trehalose, the edible fungus fiber, and the nanoscale corn starch. And
the edible fungus fiber is a mixture of the fiber of Lentinus edodes and the fiber of
Dictyophora indusiate in the mass ratio of 1:1.
[36] In this example, the mass ratio of the fructo-oligosaccharides, the soybean peptides with small molecular weight, trehalose, the edible fungus fiber, and the
nanoscale corn starch is 1:0.3:0.2:5:2.
[37] In this example, the amount of effective and viable bacteria of the probiotic
flora is > 2X 10/g, and the amount of effective and viable bacteria of the biochar
based probiotic preparation is > 2 X 10 9/g.
[38] Field tests demonstrate that the biochar-based probiotic preparation of the present disclosure can effectively improve the environment for the root growth of plants,
promote the growth of beneficial bacteria in the soil. The organic matter content in the
soil is increased by 7.63%-13.97%, the microbial biomass carbon in the soil is increased
by 27.5%-43.1%, and the microbial biomass nitrogen in the soil is increased by 33.1 %
39.7%.
[39] The specific examples of the present disclosure are described above. It shall be
understood that the present disclosure is not limited to the above specific examples, and
various changes or modifications still could be made by those skilled in the art within
the scope of the claims, without affecting the essence of the present disclosure. The
examples and the features in the examples of the present disclosure could be combined
arbitrarily without conflict.
[40] It is to be understood that, if any prior art publication is referred to herein, such reference does not constitute an admission that the publication forms a part of the
common general knowledge in the art, in Australia or any other country.
[41] In the claims which follow and in the preceding description of the invention,
except where the context requires otherwise due to express language or necessary
implication, the word "comprise" or variations such as "comprises" or "comprising" is used in an inclusive sense, i.e. to specify the presence of the stated features but not to preclude the presence or addition of further features in various embodiments of the invention.
Claims (5)
1. A biochar-based probiotic preparation, wherein, comprising a biochar pellet,
nutrient particles embedded and internally attached to the biochar pellet, and a probiotic
flora attached to surfaces of the nutrient particles and between inner walls of the nutrient
particles and the biochar pellet.
2. The biochar-based probiotic preparation according to claim 1, wherein, the
biochar pellet is prepared by the following steps:
crushing an agricultural waste by steam explosion, then pyrolyzing at 650-750°C
for 2-3 h under the protection of nitrogen, and then mixing with an appropriate amount
of solid hydrogel microparticles to granulate, to obtain the biochar pellet.
3. The biochar-based probiotic preparation according to claim 2, wherein, a particle
size of the solid hydrogel microparticles is 0.7-1 [m, and a mass ratio of the solid
hydrogel microparticles to the agricultural waste is 10:1; wherein, the solid hydrogel
microparticles are solid particles in a corresponding shape formed after a super
absorbent polymer resin in a certain shape fully absorbs water to expand.
4. The biochar-based probiotic preparation according to claim 1, wherein, a particle
size of the nutrient particles is less than 0.5 [m, and a mass ratio of the nutrient particles
to the biochar pellet is 1:5; the nutrient particles are prepared from fructo
oligosaccharides, soybean peptides with small molecular weight, trehalose, edible
fungus fiber, and nanoscale corn starch; wherein, a mass ratio of the fructo
oligosaccharides, the soybean peptides with small molecular weight, trehalose, the edible
fungus fiber, and the nanoscale corn starch is 1:0.3:0.2:5:2; wherein, an amount of
effective and viable bacteria of the probiotic flora is > 2X 10 10 /g, and an amount of
effective and viable bacteria of the biochar-based probiotic preparation is > 2X 10 9/g.
5. A preparation method of the biochar-based probiotic preparation according to
any one of claims 1-7, wherein, comprising the following steps:
S1. crushing the agricultural waste by steam explosion, then pyrolyzing at 650
750°C for 2-3 h under the protection of nitrogen, and then mixing with an appropriate
amount of the solid hydrogel microparticles to granulate, to obtain the biochar pellet with a particle size of 24-30 mesh;
S2. evenly mixing the fructo-oligosaccharides, the soybean peptides with small
molecular weight, trehalose, the edible fungus fiber, and the nanoscale corn starch in the
mass ratio of 1:0.3:0.2:5:2, then grinding, drying, and crushing, to obtain the nutrient
particles with the particle size of less than 0.5 im;
S3. putting the biochar pellet in a trundling pan of a centrifugal granulator, and
putting a paste of the nutrient particles (made by 1 part of the nutrient particles with 5
times an amount of water) in a feed chamber of a granulator equipped with a coating
system, then turning on the granulators to prepare the biochar pellet embedded and
internally attached with the nutrient particles by a powder layering method, for standby;
S4. completing an expanded culture of the probiotic flora to obtain a probiotic
solution; and
S5. putting the biochar pellet embedded and internally attached with the nutrient
particles in a fluidized bed coater for bottom spray coating with a liquid-spraying speed
of 5.0-6.0 mL/min and an air inlet frequency of 35-45 Hz, and using the probiotic
solution as a coating solution; wherein, a temperature in the fluidized bed coater is
maintained at 30°C, and the coated biochar pellet is dried in the fluidized bed coater
after the coating is completed, to obtain the biochar-based probiotic preparation.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110526736.2 | 2021-05-14 | ||
| CN202110526736.2A CN113004103A (en) | 2021-05-14 | 2021-05-14 | Biochar-based probiotic and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2021105657A4 true AU2021105657A4 (en) | 2021-10-14 |
Family
ID=76380761
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2021105657A Ceased AU2021105657A4 (en) | 2021-05-14 | 2021-08-17 | Biochar-based probiotic preparation and preparation method thereof |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN113004103A (en) |
| AU (1) | AU2021105657A4 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114042435A (en) * | 2021-11-03 | 2022-02-15 | 山东农业工程学院 | Method for preparing biochar by pretreating biomass through steam explosion |
| CN114642649B (en) * | 2022-03-22 | 2023-04-14 | 安徽安生生物化工科技有限责任公司 | Preparation method of probiotic enteric-coated granules resistant to cold and heat treatment |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH089515B2 (en) * | 1992-05-30 | 1996-01-31 | 小倉合成工業株式会社 | Anti-caking agent for fertilizer and granular fertilizer using the same |
| CN102649655B (en) * | 2012-04-27 | 2015-04-01 | 中国科学院上海高等研究院 | Carbon-based compound fertilizer containing probiotic organisms and preparation method of carbon-based compound fertilizer |
| CN104860739A (en) * | 2014-02-26 | 2015-08-26 | 上海孚祥生物科技有限公司 | Biomass charcoal-based soil conditioner and preparation method thereof |
| CN105777372B (en) * | 2016-04-05 | 2019-06-21 | 山东宝源生物科技股份有限公司 | Improve the composite microbiological fertilizer and the preparation method and application thereof of crop anti-adversity |
| CN110184073B (en) * | 2019-06-14 | 2021-01-15 | 福建农林大学 | Biochar-based water-retaining agent suitable for improving soil in hilly poor land and promoting vegetation growth and preparation method thereof |
| CN110343694A (en) * | 2019-07-17 | 2019-10-18 | 成都益盛环境工程科技有限责任公司 | A kind of preparation method of complex micro organism fungicide |
| CN110577442A (en) * | 2019-10-08 | 2019-12-17 | 吉林省汇泉农业科技有限公司 | carbon-based soil environment restoration agent and preparation method thereof |
| CN110818507A (en) * | 2019-12-03 | 2020-02-21 | 平顶山豫稼轩农业科技股份有限公司 | Bio-organic fertilizer and processing method thereof |
-
2021
- 2021-05-14 CN CN202110526736.2A patent/CN113004103A/en active Pending
- 2021-08-17 AU AU2021105657A patent/AU2021105657A4/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| CN113004103A (en) | 2021-06-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2021105657A4 (en) | Biochar-based probiotic preparation and preparation method thereof | |
| CN104311253B (en) | A kind of chitosan sustained-release fertilizer microballoon and preparation method thereof | |
| CN109400397B (en) | Water-retention controlled-release biological compound fertilizer and preparation method thereof | |
| CN113024286A (en) | Modified biochar, biochar-based organic fertilizer and preparation method thereof | |
| CN111116273A (en) | Preparation method and use method of rice straw biochar-based slow-release fertilizer | |
| CN111848291A (en) | Environment-friendly coated slow-release organic fertilizer and preparation method thereof | |
| CN110818507A (en) | Bio-organic fertilizer and processing method thereof | |
| CN109516871A (en) | A kind of preparation method of high sustained release performance charcoal base composite fertilizer | |
| CN108610190A (en) | A kind of itself sustained release alga fertilizer preparation method for capableing of repair saline-land | |
| CN104447103A (en) | Expanded vermiculite-sludge coated biological fertilizer for strawberry and preparation method of biological fertilizer | |
| CN109956824A (en) | A kind of oil tree peony organic granular slow-release fertilizer and preparation method thereof | |
| CN106588435A (en) | Organic fertilizer carrier prepared from carbonized rice hull granules and preparation method thereof | |
| CN104692914A (en) | Polymerized coated fertilizer for reducing pest and disease damage of strawberries by utilizing modified bamboo fiber | |
| CN102942409B (en) | Humic acid nutrient-gathering fertilizer and production method thereof | |
| CN110981603B (en) | Coated slow-release fertilizer for wheat and preparation method and application thereof | |
| CN102701865B (en) | Synergistic additive of chemical fertilizer and production method of synergistic additive | |
| CN109516838B (en) | Water-retention synergistic compound fertilizer and preparation method thereof | |
| CN109134054A (en) | A kind of biodegradable microballoon coat fertilizer | |
| AU2021104051A4 (en) | Microbial soil conditioner and preparation method thereof | |
| CN113265254B (en) | Microcapsule type soil conditioner containing microorganisms and preparation method and application thereof | |
| CN111454098B (en) | Double-film controlled-release fertilizer coating method | |
| CN115010538A (en) | Two-component composite microorganism water-retention temperature-control slow-release fertilizer and preparation method and application thereof | |
| CN108101626A (en) | A kind of ecology sustained release organic film fertilizer and preparation method | |
| CN107857605A (en) | One kind polymerization ureaformaldehyde method of modifying and modified poly ureaformaldehyde and its application | |
| CN114349571A (en) | Slow-release microbial organic fertilizer and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGI | Letters patent sealed or granted (innovation patent) | ||
| MK22 | Patent ceased section 143a(d), or expired - non payment of renewal fee or expiry |