AU2021102872A4 - Biospectrum based npk and trace elements enriched compost for plants - Google Patents

Abstract

This present investigation involves the biofertilisers enriched nitrogen (N), phosphate (P), potassium (K) with the Calcium and trace minerals obtained the waste from the poultry and animal husbandry industries. This formulated biospectrum based compost required to enhance the seed germination, plant height, root length and yield of commercial crops such as sunflower, wheat, paddy, groundnut, and leguminous plants. This compost is chemical free and eco-friendly for sustainable agricultural applications that are rich in carbon nitrogen ratio of soil. This preparation also known to increase porosity and water holding capacity of the soil to increase the plant yield. The formulated biospectrum based compost can be used for soil reclamation and increase the crop yield of crops. 14 Pre-grounded the poultry droppings and egg shell Bio-activators of vermi-organic wastes Commerclally available Biofertillizers Lyophilized Animal Urine Biospectrum baed NPK of Solid & Liquid formulations for large scale crop cultivation Figure: 1 Solid formulation 15 kg/ha ,4 - If. Liquid formulations 500ml of 10OX solution Vermicompost 20 kg/ha Control Figure: 2

Description

Pre-grounded the poultry droppings and egg shell

Bio-activators of vermi-organic wastes

Commerclally available Biofertillizers

Lyophilized Animal Urine

Biospectrum baed NPK of Solid & Liquid formulations for large scale crop cultivation Figure: 1

Solid formulation 15 kg/ha

,4 - If. Liquid formulations 500ml of 10OX solution

Vermicompost kg/ha Control

Figure: 2

BIOSPECTRUM BASED NPK AND TRACE ELEMENTS ENRICHED COMPOST FOR PLANTS FIELD OF INVENTION

Relates to process and formulation of agricultural/plant growth promoting microbial based material with macro and micronutrients with the trace minerals containing microbial consortium-based compost. The disclosure also relates to the methods of plant growth promotion, increased nutrient in variety of soil, improving the soil quality, plant protection and ecofriendly natural fertilizers for reducing the applications of chemical fertilizers and increase the crop yield. Thus, greatly benefit environment by using the naturally derived standardized microbial based product based on liquid and soil products for crops.

BACKGROUND

Soil fertility and organic matter management for crops, do not allow soil erosion, provide nutrients, protect soil against pH changes, water retaining capacity, reduction in soil compaction, store nutrient in soil, reduce heating of soil, increase soil health, reduce the toxic substances and ecofriendly. Invention related to the ecofriendly and plant growth enhancers formulation of natural wastes of poultry dropping and animal husbandry with the microbial consortium. Massive agriculture leaches out and nutrient depletion in soil and exhausting nutrient reserves has affected the crop yield. This resulted in the usage of chemical fertilisers to crop to accelerate the yield but resulted in poor nutritive quality of the crop. Thus, led to the soil and water contamination of conventional chemicals used in agricultural practices that is devastating the ecosystems to sustainability of life with poor human and animal health. There is need for indirect (soil/plant-based), direct application and reduction of pollutant is important for restoration of ecosystem. Effective solutions to effectively to restore the conventional depleting methods of farming, forestry and water management to sustainable and healthier practices to maintain productive yields and soil health management. Conventional chemical fertilizers used in agriculture has greater contributed to depletion of vital nutrients in food crops, soil acidification and degradation, reliance on toxic pesticides and herbicides, eutrophication of waterways and adverse climate changes. The ecological food web can become seriously compromised, resulting in "dead" rather than "living" soil. Specific impacts of chemical fertilizers include following: 1. Acidity destroys the friability of soil and increases surface compaction 2. Increased impermeability prevents rain water from entering the soil 3. Acidity adversely affects soil pH which in turn impacts beneficial microorganisms. 4. Chemicals harm earthworms, the "great aerators and conditioners of the soil" 5. Chemicals harm beneficial insects like bees and ladybugs 6. Increased plant weakness and diseases through damage to mycorrhizae fungi roots 7. Increased fungal and bacterial diseases through excessive NPK and lack of trace elements 8. Wasteful chemical leaching into ground water and water bodies 9. Agricultural run-off and sprays damage ecological balance and wildlife habitat 10. Instant uptake of water-soluble NPK can overwhelm and "burn" plant foliage 11. Lower nutritional value and less flavour in fruits, vegetables and other crop 12. Adverse impacts on climate, air quality and atmosphere

In the existing system as given in Indian Patent Application No. 85/CAL/2000- Granted Patent wherein the invention relates to a process for preparing a novel biofertilizer strains of nitrogen fixer and phosphate solubilize to promote soil health in particular for growth of tea plants comprising of nitrogen fixer and phosphate solubilize in equal proportion wherein the nitrogen fixer is prepared by the steps of a) transferring the stock culture Azotobacter Chroococcum (BAH) into mother culture modified Ashley's medium, b) allowing the mother culture medium to grow for 3-7 days, c) transferring the grown mother culture to main culture medium and d) allowing the main culture medium to grow for another 3-7 days to obtain Azotobacter choococcum (BAH) strains and in which the phosphate solubilize is prepared from as a mixture of Bacillus subtilis (BB15),Pseudomonas putida

(BP19), Aspergillus awamori (FA16) and Aspergillusflavus (FA18) in a ratio of 5:2:2:1 in Pikovskaya's medium wherein Bacillus Subtilis (BB15) and Pseudomonasputida (BP 19) are prepared as in a manner of preparing Azotobacter choococcum, Aspergillus awamori (FA16) and Aspergillus flavus (FA18) (molasses or yeast medium) are prepared by transferring the stock culture to mother culture medium, allowing the mother culture to grow for 15 days, transferring the grown mother culture to main culture medium and allowing it to grow again for another 1 days to obtain Aspergillus awamori (FA16) and Aspergillus flavus (FA18) strains. In the existing system as given in Indian Patent Application No. 781/CHENP/2008 - Patent Application, wherein the invention pertains to improved microorganisms and methods for the production of methionine and other sulfur containing the chemicals using the metL geneCO from Bacillus subtilis or a gene related to metL. In some embodiments of the present invention, the metl gene or another gene is integrated in a fashion that allows for co production of a water-soluble compound such as methionine or other amino acid and a carotenoid compound. In the existing system as given in Indian Patent Application No. 3456/DEL/2005 -Patent Application, wherein the invention pertains to the composition B5 prepared from bacterium namely B. subtilis strain B5 has the ability to provide plant growth promotion and control of soil/seed borne pathogens of potato, vegetables and ornamental crop plants. In the existing system as given in Indian Patent Application No. 163/MAS/2002 - Granted Patent wherein the invention relates to a method for preparation of a bioformulation comprising selecting the effective bio-control agents from Paecilomyces lilacinus Bainier Verticillium chlamydosporium Goddard, Trichoderma harzianum Rifai (TH-1) and TH-2, T. viride Pers. Ex. S. F. Grey, Laetisaria arvalis Burdsall, Gliocladium virens Miller, Giddens & Foster and Bacillus subtilis, preparing a medium by mixing thoroughly sorghum flour, river sand and distilled water, sterilizing the medium in an autoclave, shaking the medium to avoid clogging of the substrates, cooling the medium, inoculating the medium with said effective biocontrol agents in a flask, incubating said flask for few days, collecting the biomass of biocontrol agent along with media, mixing the said biomass of biocontrol agent and media with a carrier, drying blending the said mixtures air drying the contents and packing them in polythene bags. In the existing system as given in Indian Patent Application No. 1019/DEL/2007 - Patent Application, wherein the invention relates to a bacterial strain of the Bacillus subtilis group, wherein one or more genes involved in the biosynthesis of isovaleric acids have been modified such that the bacterium cannot produce substantial amounts of isovaleric acids. In the existing system as given in Indian Patent Application No. 340/MfUM/2010 - Granted Patent based upon composition and method of preparing an innovative bio fertilizer prepared from pure /4 culture of bacteria namely Bacillus polymexa or Bacillus subtilis or Pseudomonas putida or Pseudomonas fluorescens for providing rich nutrients to plant through soil and thereby promoting plant growth. In the existing system as given in International Patent Application No. PCT/CA2016/050657 - granted A bioavailable mineral fertilizer composition comprising glacial gravel dust, basalt powder, phosphate, calcium carbonate, potassium, seawater solids, fulvic acid, humic acid, bentonite clay, endo or ectomycorrhizae or other fungi spores, kelp meal powder, microalgae and/or other protein powder, bacteria and/or yeast, wheat bran or stabilized rice bran, and organic blackstrap molasses powder. In the existing system as given in International patent application No. PCT/TR20 18/050450-granted for the preparation of a soil inoculating composition comprising the steps of a) loading a vermicompost into a tank, b) adding a sufficient amount of water and molasses to vermicompost, c) breeding of microorganism in the mixture obtained in the step b), by adjusting the temperature to a range between 25-27C and by providing air into the mixture, d) forcing microorganism in the mixture obtained in the step c), to dormant state by d) adding a sufficient amount of molasses, e) bottling the composition obtained in the step d), for storage, f) resuscitating of microorganism in the stored composition from the dormant state to active state by diluting the composition with a sufficient amount of water before soil inoculation. In the existing system as given in International patent application No. 14/161,424/2014 granted for the present invention relates to eco-friendly compositions and methods for providing plant growth enhancing formulations comprising mixtures of microbial isolates. In particular, numerous bacterial and fungal strains were isolated from a variety of soil types, from rhizospheres and from root nodules of leguminous plants, in designed combinations, for providing plant growth and plant productivity enhancing formulations. These specifically designed polymicrobial formulations would further provide protection against plant pathogens lowering the need for nitrogen containing fertilizers, solubilize minerals, protect plants against pathogens, and s make available to the plant valuable 10 nutrients, such as phosphate, thus reducing and eliminating the need for using chemical fertilizers and chemical pesticides. In the existing system as given in International patent application No. 15/971, 513/2019 Granted for fertilizer and method of making a fertilizer that include bioactivated nutrients derived from immobile/fewer mobile elements. The immobile/less elements are made available to plants by the action of element mobilizing microorganisms, which are isolated, cultured and combined with organic material and sources of the immobile element. The major key present invention is to rebuilds the foundations of agriculture through soil restoration with new class of non-toxic, eco-friendly, user friendly, Biomineral based products with several applications for agriculture, forestry, water management, industrial detoxification and plant-soil health supplements with providing a broad-range minerals and other nutrients essentials to good health along with the NPK. Further, they improve soil structure/water retention, reduce toxic contaminants and support the ecological balance. Also, enhance the nutrient uptake, climate resilience, increase plant immunity and maintaining product purity and safety (Without animal byproducts).

OBJECT OF THE INVENTION

Naturally derived standardized microbial based product based on liquid and solid products for crops. STATEMENT OF INVENTION

The inventor has invented solid and liquid composition/formulation and method involved in production for organic novel highly nutrient rich and plant growth enhancing microbial consortium products for plants.

To further clarify advantages and features of the present disclosure, a more particular description of the invention will be rendered by reference to specific embodiments thereof, which is illustrated in the appended drawings. It is appreciated that these drawings depict only typical embodiments of the invention and are therefore not to be considered limiting of its scope. The invention will be described and explained with additional specificity and detail with the accompanying drawings.

BRIEF DESCRIPTION OF THE DRAWINGS

These and other features, aspects, and advantages of the present disclosure will become better understood when the following detailed description is read with reference to the accompanying drawings in which like characters represent like parts throughout the drawings, wherein: The present disclosure in accordance with results of three important crops is described in detail in the following figures. The drawings provided for the illustrations and depicting the examples of few crops only. These facilitate the better understanding of the disclosure. Figure 1 is illustration of Bio-stimulant based biofertilizer NPK formulations and preparations Figure 2 is illustration of field trail of the solid and liquid formulation of the biofertilizers The present disclosure in accordance with results of three important crops is described in detail in the following tables along with the vermicompost as positive controls. These tables provided for the comparative and novelty of the preparations. These facilitate the better understanding of the disclosure of the process and product development. Figure 3: Table No. 1 Green house formulated composition plant growth of 4-weeks using pot trials. Figure 4: Table No. 2 In vitro and in vivo pesticide carbendazim (CBZ) degrading potentials of formulations. Figure 5: Table No. 3 Plant growth promoting traits of formulated products in the soil. Figure 6: Table No. 4 Nutrient status of the field trails before and after treatment with formulations.

Further, skilled artisans will appreciate that elements in the drawings are illustrated for simplicity and may not have necessarily been drawn to scale. For example, the flow charts illustrate the method in terms of the most prominent steps involved to help to improve understanding of aspects of the present disclosure.

DETAILED DESCRIPTION OF INVENTION

For the purpose of promoting an understanding of the principles of the invention, reference will now be made to the embodiment illustrated in the drawings and specific language will be used to describe the same. It will nevertheless be understood that no limitation of the scope of the invention is thereby intended, such alterations and further modifications in the illustrated system, and such further applications of the principles of the invention as illustrated therein being contemplated as would normally occur to one skilled in the art to which the invention relates. It will be understood by those skilled in the art that the foregoing general description and the following detailed description are exemplary and explanatory of the invention and are not intended to be restrictive thereof. Reference throughout this specification to "an aspect", "another aspect" or similar language means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment of the present disclosure. Thus, appearances of the phrase "in an embodiment", "in another embodiment" and similar language throughout this specification may, but do not necessarily, all refer to the same embodiment. The terms "comprises", "comprising", or any other variations thereof, are intended to cover a non-exclusive inclusion, such that a process or method that comprises a list of steps does not include only those steps but may include other steps not expressly listed or inherent to such process or method. Similarly, one or more devices or sub-systems or elements or structures or components proceeded by "comprises...a" does not, without more constraints, preclude the existence of other devices or other sub-systems or other elements or other structures or other components or additional devices or additional sub-systems or additional elements or additional structures or additional components. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Vermicast, worm castings contain worm humus or worm manure, after decomposition of organic wastes by the earthworm and its associated natural microbiome. These types of the fertilizers are reported to reduce the contaminants and increase the soil fertility through decomposition and soil conditioning. Mixing the PGPRs with vermicast and poultry dropping and finely grounded egg shells seems to enhance the aeration, water holding capacity and controlled release of nutrient during decomposition by PGPRs. Hence, the these biospectrum based NPK and trace elements enriched compost for plants. By mixing vermicast with powdered form of poultry waste and egg shells with commercially available suitable microbes that has enzymes will create a premium top dressing/ landscaping product. Hence, there is a direct positive impact on the soil health, plant growth, economical, eco-friendly and cost-effective. Liquid formulation is suitable for greenhouses, hydroponics, vertical gardens, foliar spray and other liquid agricultural and agro-forestry applications. The liquid formulation can be impregnated as granule form that could be innovative modification similar to urea application in the field. Any natural based food grade materials that include alignate, biodegradable polymers, molasses, natural gums and wax can be used for granule formulation. These granules do contain the PGPRs mentioned and they can be used for the seed coating as well as direct applications. Microbial inoculations include following Azospirillum brasilense - free-living Nitrogen fixer, converts atmospheric N, produces growth promoting substances like indole acetic acid and gibberellins Bacillus varieties - N fixer, nutrient cycling, decomposition, enzymes, soil conditioning and structure Pseudomonas - protect plants against pathogenic fungi in root zone The innovation is three-fold relating the novel formulations and manufacturing process with extensive range of usage that transcends the conventional products. Biominerals with multi functional product with a wide range of derivative requiring whole systems thinking and multi-disciplinary synthesis. The product and process accelerate and enhance soil building and other natural processes which provide excellent solutions for agriculture and environment. These product increase plant yields, neutralises the contaminants, protect the ecological food web and replenisj vital nutrients. All the formulations are natural macro and micronutrients, microorganisms, carbon rich and biostimulators to promote nutritional and plant health. They are formulated in solid and liquid forms that makes bioavailable to plants. Figure 1 Illustration of Bio-stimulant based biofertilizer NPK formulations and preparations Figure 2 Illustration of field trail of the solid and liquid formulation of the biofertilizers The present disclosure in accordance with results of three important crops is described in detail in the following tables along with the vermicompost as positive controls. These tables provided for the comparative and novelty of the preparations. These facilitate the better understanding of the disclosure of the process and product development. Figure 3: Table No. 1 Green house formulated composition plant growth of 4-weeks using pot trials Figure 4: Table No. 2 In vitro and in vivo pesticide carbendazim (CBZ) degrading potentials of formulations Figure 5: Table No. 3 Plant growth promoting traits of formulated products in the soil Figure 6: Table No. 4 Nutrient status of the field trails before and after treatment with formulations Manufacturing process for liquid concentrate will also incorporate vital life force technology that further improves the bioavailability of the core mineral-fulvic complex. This vital force technology imprints subtle energetic information to enhance the cellular functions and to restore harmonious equilibrium of plants, animals and humans. For example, in agricultural applications, the information imprints can be derived from essential mineral types, flowers/herbs, periodic elements and environmental forces that are vital to soil health, aerobic processes of decomposition and root growth. The composite bio-minerals product could also be used as a seed-starting mix and compost booster for crops and landscaping applications and should enhance the survivability

(germination and growth) of woody plant seeds in reforestation/replanting applications. The present invention discloses a method for producing agri-nutri solid and liquid compost, comprising: biofertilizer of commercially available such as Bacillus amyloliquefaciens (ATCC3978) Pseudomonas fluorscens (ATCC11253), Pseudomonas putida (ATCC11172), Bacillus subtilis (ATCC2107), Azospirillum brasilense (ATCC35253) and VAM with a heat-treated goat and poultry faecal wastes for plant growth. In another embodiment, the present invention discloses a method for producing comprising solid compost, wherein grounded powder of sun dried/heat treated fecal at the balanced ratio contained mentioned microorganisms with certain proactive natural enzymes and phytohormones that increased the crop yield and plant growth than the commercial biofertilizers. The liquid compost comprising of the concentrated solution of extracted and clarified from animal urine with the sun dried fecal that increased the crop yield and plant growth than the commercial biofertilizers. The liquid compost is stable for several months suitable for the home-made plants to the commercial crops. The liquid compost is stable preparation to be diluted to several folds for the home-made plants to the commercial crops. Present investigation provides multi-microbial formulations when said comprises of at least four microbes. The product of nutritive solid and liquid for several crop plants was table micro- and micronutrients Nitrogen content of 0.9 to 1.2% of Carbon, Phosphorous of 1.16 to 2.0% of Carbon, Potassium of 1.35 to 2.3% of Carbon and other minerals of 1 to 1.8% of Carbon. Intense grinding and blending varied with the powder form of the granules of particle size ranging from 0.1 to 0.5 mm using above referred raw materials. The formulated product contained N, P, K, Ca, P and other mineral composition with technical specification of fabricated natural form of biofertilizers which provided consumption of 45% of Carbon and % of other essential nutrients with microbes, enzymes and natural metabolites. The Microbial consortium is the fermented culture in combination of Azospirillum brasilense (ATCC35253), Pseudomonas fluorscens (ATCC11253), Pseudomonas putida (ATCC11172) and Bacillus amyloliquefaciens (ATCC3978). Effective Microorganisms (EM) are consortia brelong to lactic acid bacteria, purple phototrophic bacteria and yeast with a range of agricultural, forestry and environmental remediation applications. The Formulated solid and liquid preparations had reduced the pesticide carbendazim used in agriculture as toxic pesticides.

The drawings and the forgoing description give examples of embodiments. Those skilled in the art will appreciate that one or more of the described elements may well be combined into a single functional element. Alternatively, certain elements may be split into multiple functional elements. Elements from one embodiment may be added to another embodiment. For example, orders of processes described herein may be changed and are not limited to the manner described herein. Moreover, the actions of any flow diagram need not be implemented in the order shown; nor do all of the acts necessarily need to be performed. Also, those acts that are not dependent on other acts may be performed in parallel with the other acts. The scope of embodiments is by no means limited by these specific examples. Numerous variations, whether explicitly given in the specification or not, such as differences in structure, dimension, and use of material, are possible. The scope of embodiments is at least as broad as given by the following claims.

Claims (10)

1. WE CLAIM 1. A method for producing agri-nutri solid and liquid compost, comprising: biofertilizer of commercially available such as Bacillus amyloliquefaciens (ATCC3978) Pseudomonas fluorscens (ATCC11253), Pseudomonas putida (ATCC11172), Bacillus subtilis (ATCC2107), Azospirillum brasilense (ATCC35253) and VAM with a heat-treated goat and poultry faecal wastes for plant growth.
2. 2. A method for producing comprising solid compost as claimed in claim 1, wherein grounded powder of sun dried/heat treated fecal at the balanced ratio contained mentioned microorganisms with certain proactive natural enzymes and phytohormones that increased the crop yield and plant growth than the commercial biofertilizers
3. 3. The method for producing the liquid compost as claimed in claim 1 comprising of the concentrated solution of extracted and clarified from animal urine with the sun dried fecal that increased the crop yield and plant growth than the commercial biofertilizers
4. 4. The method for producing the solid compost as claimed in claim 2, wherein is stable for several months suitable for the home-made plants to the commercial crops
5. 5. The method for producing the liquid compost as claimed in claim 3, wherein is stable preparation to be diluted to several folds for the home-made plants to the commercial crops. Present investigation provides multi-microbial formulations when said comprises of at least four microbes.
6. 6. The product of nutritive solid and liquid for several crop plants as claimed 1-4 was table micro- and micronutrients Nitrogen content of 0.9 to 1.2% of Carbon, Phosphorous of 1.16 to 2.0% of Carbon, Potassium of 1.35 to 2.3% of Carbon and other minerals of I to 1.8% of Carbon.
7. 7. Intense grinding and blending varied with the powder form of the granules of particle size ranging from 0.1 to 0.5 mm using the raw materials as claimed 1-5.
8. 8. The formulated product contained N, P, K, Ca, P and other mineral composition with technical specification of fabricated natural form of biofertilizers which provided consumption of 45% of Carbon and 55% of other essential nutrients with microbes, enzymes and natural metabolites.
9. 9. Microbial consortium claimed in 5 and 8 is the fermented culture in combination of Azospirillum brasilense (ATCC35253), Pseudomonas fluorscens (ATCC11253), Pseudomonas putida (ATCC11172) and Bacillus amyloliquefaciens (ATCC3978). Effective Microorganisms (EM) are consortia brelong to lactic acid bacteria, purple phototrophic bacteria and yeast with a range of agricultural, forestry and environmental remediation applications.
10. 10. Formulated solid and liquid preparations had reduced the pesticide carbendazim used in agriculture as toxic pesticides.

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