AU2020262117B2 - Cell culture medium for eukaryotic cells - Google Patents

Cell culture medium for eukaryotic cells

Info

Publication number
AU2020262117B2
AU2020262117B2 AU2020262117A AU2020262117A AU2020262117B2 AU 2020262117 B2 AU2020262117 B2 AU 2020262117B2 AU 2020262117 A AU2020262117 A AU 2020262117A AU 2020262117 A AU2020262117 A AU 2020262117A AU 2020262117 B2 AU2020262117 B2 AU 2020262117B2
Authority
AU
Australia
Prior art keywords
cell culture
medium
culture medium
acid
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2020262117A
Other languages
English (en)
Other versions
AU2020262117A1 (en
Inventor
John Chen
Nathaniel GOLDEN
Theodore LONEY
Carolyn Scott
Wei Xue
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Regeneron Pharmaceuticals Inc
Original Assignee
Regeneron Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=70681881&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AU2020262117(B2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Regeneron Pharmaceuticals Inc filed Critical Regeneron Pharmaceuticals Inc
Publication of AU2020262117A1 publication Critical patent/AU2020262117A1/en
Application granted granted Critical
Publication of AU2020262117B2 publication Critical patent/AU2020262117B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/71—Receptors; Cell surface antigens; Cell surface determinants for growth factors; for growth regulators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0043—Medium free of human- or animal-derived components
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/40—Nucleotides, nucleosides or bases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/46—Amines, e.g. putrescine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/92—Medium free of human- or animal-derived components

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2020262117A 2019-04-23 2020-04-22 Cell culture medium for eukaryotic cells Active AU2020262117B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962837263P 2019-04-23 2019-04-23
US62/837,263 2019-04-23
PCT/US2020/029370 WO2020219582A1 (en) 2019-04-23 2020-04-22 Cell culture medium for eukaryotic cells

Publications (2)

Publication Number Publication Date
AU2020262117A1 AU2020262117A1 (en) 2021-11-11
AU2020262117B2 true AU2020262117B2 (en) 2026-02-12

Family

ID=70681881

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2020262117A Active AU2020262117B2 (en) 2019-04-23 2020-04-22 Cell culture medium for eukaryotic cells

Country Status (15)

Country Link
US (5) US10961500B1 (https=)
EP (1) EP3959234A1 (https=)
JP (2) JP2022529485A (https=)
KR (1) KR20220003563A (https=)
CN (1) CN113710696A (https=)
AU (1) AU2020262117B2 (https=)
BR (1) BR112021020501A2 (https=)
CA (1) CA3136827A1 (https=)
EA (1) EA202192893A1 (https=)
IL (1) IL287387A (https=)
MX (1) MX2021012649A (https=)
MY (1) MY198223A (https=)
SG (1) SG11202111252QA (https=)
WO (1) WO2020219582A1 (https=)
ZA (1) ZA202107808B (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10961500B1 (en) * 2019-04-23 2021-03-30 Regeneron Pharmaceuticals, Inc. Cell culture medium for eukaryotic cells
WO2023278525A1 (en) * 2021-06-29 2023-01-05 Biomarin Pharmaceutical Inc. Cell culture feed for recombinant adeno-associated virus production in mammalian cells
CN118240749B (zh) * 2024-05-28 2024-08-06 烟台赛普生物技术有限公司 一种灌流培养基及其制备方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030096414A1 (en) * 2001-03-27 2003-05-22 Invitrogen Corporation Culture medium for cell growth and transfection
US20060148074A1 (en) * 1996-08-30 2006-07-06 Invitrogen Corporation Serum-free mammalian cell culture medium, and uses thereof
US20120035110A1 (en) * 2010-07-08 2012-02-09 Baxter Healthcare S.A. METHOD OF PRODUCING RECOMBINANT HIGH MOLECULAR WEIGHT vWF IN CELL CULTURE
WO2017024062A1 (en) * 2015-08-04 2017-02-09 Regeneron Pharmaceuticals, Inc. Taurine supplemented cell culture medium and methods of use
WO2019010191A1 (en) * 2017-07-06 2019-01-10 Regeneron Pharmaceuticals, Inc. CELL CULTURE METHOD FOR PRODUCING GLYCOPROTEIN
EP3431588A1 (en) * 2009-08-11 2019-01-23 F. Hoffmann-La Roche AG Production of proteins in glutamine-free cell culture media

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1312030C (en) * 1987-11-18 1992-12-29 Brian Maiorella Method to increase antibody titer
US6406909B1 (en) * 1998-07-10 2002-06-18 Chugai Seiyaku Kabushiki Kaisha Serum-free medium for culturing animal cells
AT409379B (de) * 1999-06-02 2002-07-25 Baxter Ag Medium zur protein- und serumfreien kultivierung von zellen
US7087411B2 (en) * 1999-06-08 2006-08-08 Regeneron Pharmaceuticals, Inc. Fusion protein capable of binding VEGF
SG106672A1 (en) 2002-03-08 2004-10-29 Asml Netherlands Bv Mask for use in lithography, method of making a mask, lithographic apparatus, and device manufacturing method
ATE541919T1 (de) * 2005-02-11 2012-02-15 Novo Nordisk Healthcare Ag Herstellung eines proteins in serumfreier zellkultur, die ein proteinhydrolysat aus pflanzen enthält
WO2008154014A2 (en) * 2007-06-11 2008-12-18 Amgen Inc. A method for culturing mammalian cells to improve recombinant protein production
BRPI0919034A8 (pt) * 2008-09-26 2016-02-10 Schering Corp Métodos para produzir uma proteína e um anticorpo, meio líquido aquoso para cultura, e, vaso
US9677105B2 (en) * 2013-03-14 2017-06-13 Momenta Pharmaceuticals, Inc. Methods of cell culture
AR095196A1 (es) * 2013-03-15 2015-09-30 Regeneron Pharma Medio de cultivo celular libre de suero
US10961500B1 (en) * 2019-04-23 2021-03-30 Regeneron Pharmaceuticals, Inc. Cell culture medium for eukaryotic cells

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060148074A1 (en) * 1996-08-30 2006-07-06 Invitrogen Corporation Serum-free mammalian cell culture medium, and uses thereof
US20030096414A1 (en) * 2001-03-27 2003-05-22 Invitrogen Corporation Culture medium for cell growth and transfection
EP3431588A1 (en) * 2009-08-11 2019-01-23 F. Hoffmann-La Roche AG Production of proteins in glutamine-free cell culture media
US20120035110A1 (en) * 2010-07-08 2012-02-09 Baxter Healthcare S.A. METHOD OF PRODUCING RECOMBINANT HIGH MOLECULAR WEIGHT vWF IN CELL CULTURE
WO2017024062A1 (en) * 2015-08-04 2017-02-09 Regeneron Pharmaceuticals, Inc. Taurine supplemented cell culture medium and methods of use
WO2019010191A1 (en) * 2017-07-06 2019-01-10 Regeneron Pharmaceuticals, Inc. CELL CULTURE METHOD FOR PRODUCING GLYCOPROTEIN

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Savarese, Todd M., et al. "Conversion of 5′-deoxy-5′-methylthioadenosine and 5′-deoxy-5′-methylthioinosine to methionine in cultured human leukemic cells." Cancer research 43.10 (1983): 4699-4702. *

Also Published As

Publication number Publication date
JP2025028979A (ja) 2025-03-05
US11821001B2 (en) 2023-11-21
MX2021012649A (es) 2021-11-12
KR20220003563A (ko) 2022-01-10
SG11202111252QA (en) 2021-11-29
US20230114292A1 (en) 2023-04-13
MY198223A (en) 2023-08-14
ZA202107808B (en) 2024-11-27
US10961500B1 (en) 2021-03-30
EP3959234A1 (en) 2022-03-02
IL287387A (en) 2021-12-01
US20210261913A1 (en) 2021-08-26
US20210071135A1 (en) 2021-03-11
EA202192893A1 (ru) 2022-02-03
JP2022529485A (ja) 2022-06-22
US11555176B2 (en) 2023-01-17
AU2020262117A1 (en) 2021-11-11
US20220177832A1 (en) 2022-06-09
US20240084248A1 (en) 2024-03-14
US11286460B2 (en) 2022-03-29
WO2020219582A1 (en) 2020-10-29
CN113710696A (zh) 2021-11-26
BR112021020501A2 (pt) 2021-12-07
CA3136827A1 (en) 2020-10-29

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