AU2020101001A4 - Imperata cylindrica cultivation method for soil remediation - Google Patents
Imperata cylindrica cultivation method for soil remediation Download PDFInfo
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- AU2020101001A4 AU2020101001A4 AU2020101001A AU2020101001A AU2020101001A4 AU 2020101001 A4 AU2020101001 A4 AU 2020101001A4 AU 2020101001 A AU2020101001 A AU 2020101001A AU 2020101001 A AU2020101001 A AU 2020101001A AU 2020101001 A4 AU2020101001 A4 AU 2020101001A4
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- imperata cylindrica
- rhizomes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
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- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Soil Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Cultivation Of Plants (AREA)
- Processing Of Solid Wastes (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention discloses an Imperata cylindrica cultivation method for soil
remediation in a mining area. A specific nutrient solution is prepared to promote fast growth of
Imperata cylindrica in the mining area to increase the vegetative cover of the mining area.
Imperata cylindrica rhizomes undergo soaking treatment such that acetyl tributyl citrate adheres
to the Imperata cylindrica rhizomes to improve the permeability of cell membranes. In the
present invention, a specific ionic solution is also prepared, which can not only gather ions in soil,
but also smoothly pass through cell walls to be absorbed by the Imperata cylindrica for
enrichment of metal ions and soil remediation. The Imperata cylindrica cultivation method of the
present invention is stable and controllable in process and suitable for industrial and mass
production.
Description
The present invention relates to an Imperata cylindrica (L.) P. Beauv. cultivation method for soil remediation, belonging to the technical field of environmental protection.
As China has been striving to develop its industry since the reform and opening up, mineral resources have been exploited in a large scale. Due to poor economic and technical conditions, many mining areas have been over-exploited, leaving local soil severely damaged. Surface mining has directly led to the pollution of the ecological environment surrounding mines as well as the destruction of soil, vegetation and water sources. Mining waste and rocks produced in the mining process accumulate and occupy a large amount of land randomly so that the soil contains lots of heavy metals, has been significantly diminished in utilization rate or even becomes unusable.
In addition, since mining companies discharge a large amount of wine water, which can dissolve and erode soil, as well as smoke and dust in their mining process, soil in the areas and farmlands surrounding the mines can be severely contaminated. Underground mining may render underground voids which tend to trigger ground collapse in a large area. Besides, underground mining may also cause damage to the underground rock structure and water circulation system as some groundwater sources are cut off for water drainage in some pits. For this reason, lots of wells become dried up and water resources face depletion, making the groundwater-rich areas water-scarce. Waste rock, tailings and mine water, three types of waste materials generated during mining, may also lead to the sedimentation of waste residue and sewage in the rivers around the mining areas, resulting in massive industrial pollution of water quality and quality-induced water shortage. Surface mining may incur the stripping of surface soil of the mines, and the accumulation of the resultant waste rock and tailings can degrade the vegetation that grow on the soil surface. Therefore, the vegetation cannot grow normally anymore, which means the vegetation cover and certain varieties of vegetation will be reduced, leading to ecological imbalance, and over time, desertification and widespread soil erosion.
With the progress and development of the society, soil pollution has garnered increasing attention. The issue of how to remedy the soil in the mining areas is particularly important. Currently, there are chemical, physical, and biological methods generally adopted in China to restore the contaminated soil, but each of them comes with their own defects. The present invention leverages an Imperata cylindrica cultivation method to remedy soil, which can not only regulate the pH, metal ions and other elements in the soil, but also stabilize the soil to prevent its desertification and mudslides. Imperata cylindrica, a species of perennial rhizomatous grass, features a strong root system that can stabilize the soil and provide the soil great protection. However, Imperata cylindrica does not grow well in the mining areas, and also does not have a good capacity for enrichment of metal ions. Therefore, the development of an Imperata cylindrica cultivation method which helps the Imperata cylindrica grow in the mining areas and also enrich metal ions is bound to obtain great economic and social benefits.
To solve the technical problems in prior art, the present invention provides an Imperata cylindrica cultivation method for soil remediation in a mining area. The method can help Imperata cylindrica grow in the contaminated mining area and enrich metal irons to remedy soil in the mining area.
To achieve the above objective, an embodiment includes the following steps of:
drying healthy Imperata cylindrica rhizomes at 30-40°C until the moisture is 60-80%, soaking the dried Imperata cylindrica rhizomes in a soaking solution for 24-48 h, taking out and air-drying the Imperata cylindrica rhizomes at 20-30°C.
Preferably, the soaking solution includes polyoxyethylene, acetyl tributyl citrate and water in a mass ratio of 1:1:100.
Preferably, the soaking solution is prepared by steps of heating a proper amount of water to -70°C with stirring, adding polyoxyethylene and acetyl tributyl citrate, stirring for 2-4 h, and then cooling to room temperature.
The embodiment also includes steps of digging ditches with a depth of 5-10 cm around a mining area, planting the Imperata cylindrica rhizomes in the ditches, and spraying a nutrient solution in an amount of 3 kg/mu once every half a month, and after the Imperata cylindrica grows to 15-30 cm high, spraying an ionic test solution in an amount of 4 kg/mu for six consecutive months.
Preferably, the nutrient solution includes decayed plant extract, hydroxyethyl cellulose, triethyl citrate, propylene glycol, ammonium carbonate, potassium sulfate and water in a mass ratio of 25:12:8:2:0.3:0.3:52.
Further, a method for preparing the nutrient solution includes the following steps of:
1. heating a proper amount of water to 60-70°C, adding hydroxyethyl cellulose and triethyl citrate, vacuuming to -0.6 Mpa to -0.8 Mpa, and then holding for 2-4 h;
2. adding propylene glycol, ammonium carbonate and potassium sulfate, stirring for 2-4 h, adding the decayed plant extract, and continuously stirring for 1-3 h.
Preferably, the ionic test solution includes polyoxyethylene, EDTA, acetyl tributyl citrate and water in a mass ratio of 1:2:1:50.
Further, a method for preparing the ionic test solution includes the following steps of:
dissolving EDTA in water with stirring, heating to 60-70°C, adding polyoxyethylene and acetyl tributyl citrate, stirring for 2-4 h, and cooling to room temperature.
The present invention can promote fast growth of Imperata cylindrica in the mining areas through preparation of the specific nutrient solution to change the vegetative cover of the mining areas. Besides, the present invention features soaking of the Imperata cylindrica rhizomes. Polyoxyethylene with high viscosity and hydrophilicity can help acetyl tributyl citrate well adhere to the Imperata cylindrica rhizomes, and highly fat-soluble acetyl tributyl citrate can fuse with cell membranes and cell walls to improve the permeability of the cell membranes. Moreover, the present invention allows acetyl tributyl citrate and EDTA to be effectively combined by the bridging effect of polyoxyethylene to form a composite, which can not only gather metal ions by complexing, but also be absorbed by the Imperata cylindrica rhizomes soaked in the soaking solution.
Beneficial effects of the present invention:
1. The present invention provides an Imperata cylindrica cultivation method for soil remediation, which can not only promote growth of the Imperata cylindrica in the mining area, but also help the Imperata cylindrica enrich metal ions to further improve the soil quality in the mining area.
2. The Imperata cylindrica cultivation method of the present invention is simple, controllable and suitable for industrial and mass production.
To facilitate understanding the present invention, the invention will now be described in further detail with reference to examples which should not be construed as further limit to the present invention.
Example 1
1. Healthy Imperata cylindrica rhizomes were dried at 35°C until the moisture was about %. The dried Imperata cylindrica rhizomes were soaked for 36 h in a soaking solution which included polyoxyethylene, acetyl tributyl citrate and water in a mass ratio of 1:1:100, then taken out, and air-dried at 25°C.
2. Ditches with a depth of 8 cm were dug around a mining area. The Imperata cylindrica rhizomes were planted in the ditches and sprayed with a nutrient solution in an amount of 3 kg/mu once every half a month. The nutrient solution included decayed plant extract, hydroxyethyl cellulose, triethyl citrate, propylene glycol, ammonium carbonate, potassium sulfate and water in a mass ratio of 25:12:8:2:0.3:0.3:52. After the Imperata cylindrica grew to 15-30 cm high, an ionic test solution was sprayed in an amount of 4 kg/mu for six consecutive months. The ionic test solution included polyoxyethylene, EDTA, acetyl tributyl citrate and water in a mass ratio of 1:2:1:50.
Example 2
1. Healthy Imperata cylindrica rhizomes were dried at 30°C until the moisture was 6 0 - 8 0 %.
The dried Imperata cylindrica rhizomes were soaked for 24 h in the soaking solution which included polyoxyethylene, acetyl tributyl citrate and water in the mass ratio of 1:1:100, then taken out and air-dried at 20°C.
2. Ditches with a depth of 5 cm were dug around a mining area. The Imperata cylindrica rhizomes were planted in the ditches and sprayed with a nutrient solution in an amount of 3 kg/mu once every half a month. The nutrient solution included decayed plant extract, hydroxyethyl cellulose, triethyl citrate, propylene glycol, ammonium carbonate, potassium sulfate and water in a mass ratio of 25:12:8:2:0.3:0.3:52. After the Imperata cylindrica grew to 15-30 cm high, an ionic test solution was sprayed in an amount of 4 kg/mu for six consecutive months. The ionic test solution included polyoxyethylene, EDTA, acetyl tributyl citrate and water in a mass ratio of 1:2:1:50.
Example 3
1. Healthy Imperata cylindrica rhizomes were dried at 40°C until the moisture was about %. The dried Imperata cylindrica rhizomes were soaked for 48 h in the soaking solution which included polyoxyethylene, acetyl tributyl citrate and water in the mass ratio of 1:1:100, then taken out and air-dried at 30°C.
2. Ditches with a depth of 10 cm were dug around a mining area. The Imperata cylindrica rhizomes were planted in the ditches and sprayed with a nutrient solution in an amount of 3 kg/mu once every half a month. The nutrient solution included decayed plant extract, hydroxyethyl cellulose, triethyl citrate, propylene glycol, ammonium carbonate, potassium sulfate and water in a mass ratio of 25:12:8:2:0.3:0.3:52. After the Imperata cylindrica grew to 15-30 cm high, an ionic test solution was sprayed in an amount of 4 kg/mu for six consecutive months. The ionic test solution included polyoxyethylene, EDTA, acetyl tributyl citrate and water in a mass ratio of 1:2:1:50.
Reference Example 1
1. Healthy Imperata cylindrica rhizomes were dried at 35°C until the moisture was about %. The dried Imperata cylindrica rhizomes were soaked in water for 36 h, then taken out and air-dried at 25°C.
2. Ditches with a depth of 8 cm were dug around a mining area. The Imperata cylindrica rhizomes was planted in the ditches and sprayed with a nutrient solution in an amount of 3 kg/mu once every half a month. The nutrient solution included decayed plant extract, hydroxyethyl cellulose, triethyl citrate, propylene glycol, ammonium carbonate, potassium sulfate and water in a mass ratio of 25:12:8:2:0.3:0.3:52. After the Imperata cylindrica grew to 15-30 cm high, an ionic test solution was sprayed in an amount of 4 kg/mu for six consecutive months. The ionic test solution included polyoxyethylene, EDTA, acetyl tributyl citrate and water in a mass ratio of 1:2:1:50.
Reference Example 2
1. Healthy Imperata cylindrica rhizomes were dried at 35°C until the moisture was about %. The dried Imperata cylindrica rhizomes were soaked for 36 h in a soaking solution which included polyoxyethylene, acetyl tributyl citrate and water in a mass ratio of 1:1:100, then taken out and air-dried at 25°C.
2. Ditches with a depth of 8 cm were dug around a mining area. The Imperata cylindrica rhizomes were planted in the ditches and sprayed with a nutrient solution in an amount of 3 kg/mu once every half a month. The nutrient solution included decayed plant extract, hydroxyethyl cellulose, triethyl citrate, propylene glycol, ammonium carbonate, potassium sulfate and water in a mass ratio of 25:12:8:2:0.3:0.3:52. After the Imperata cylindrica grew to 15-30 cm high, spray was stopped.
Reference Example 3
1. Healthy Imperata cylindrica rhizomes were dried at 35°C until the moisture was about %. The dried Imperata cylindrica rhizomes were soaked in water for 36 h, then taken out and air-dried at 25°C.
2. Ditches with a depth of 8 cm were dug around a mining area. The Imperata cylindrica rhizomes were planted in the ditches and sprayed with a nutrient solution in an amount of 3 kg/mu once every half a month. The nutrient solution included decayed plant extract, hydroxyethyl cellulose, triethyl citrate, propylene glycol, ammonium carbonate, potassium sulfate and water in a mass ratio of 25:12:8:2:0.3:0.3:52. After the Imperata cylindrica grew to 15-30 cm high, spray was stopped.
Investigation on oil remediation
A piece of land without plants near the mining area was flatted and divided into six sections. The sections, each with an area of 5 square meters, were numbered from 1 to 6. The Imperata cylindrica was uniformly planted in the six sections according to the examples 1-3 and the reference examples 1-3. The content of the metal ions in soil was tested using the method as provided in the standard GB 15618-2018 in the 0th month, 12th month and 2 4th month respectively to investigate oil remediation. The specific results (mg/kg) are as follows:
Number of
section of 1 2 3 4 5 6
land
Reference Reference Reference Example pH Example 1 Example 2 Example 3 Example 1 Example 2 Example 3
Cd: 9.2 Cd: 9.3 Cd: 9.6 Cd: 10.2 Cd: 9.7 Cd: 9.4
Hg: 11.3 Hg: 11.6 Hg: 11.9 Hg: 12.1 Hg: 10.9 Hg: 12.1
0 5.1 As: 152.3 As: 158.2 As: 159.1 As: 148.2 As: 161.4 As: 158.2
Pb: 1655.7 Pb: 1649.1 Pb: 1692.5 Pb: 1682.3 Pb: 1672.1 Pb: 1648.3
Cr: 1799.1 Cr: 1856.2 Cr: 1752.8 Cr: 1816.7 Cr: 1826.9 Cr: 1816.1
Cd: 1.5 Cd: 1.1 Cd: 1.2 Cd: 9.2 Cd: 9.0 Cd: 8.6
Hg: 2.4 Hg: 2.7 Hg: 2.4 Hg: 10.2 Hg: 10.1 Hg: 11.2
12th month 6.5 As: 23.5 As: 28.1 As: 23.6 As: 132.1 As: 145.1 As: 135.7
Pb: 265.1 Pb: 249.2 Pb: 262.9 Pb: 1666.2 Pb: 1651.6 Pb: 1601.3
Cr: 319.4 Cr: 322.5 Cr: 339.38 Cr: 1771.4 Cr: 1761.6 Cr: 1731.6
Cd: 0.4 Cd: 0.3 Cd: 0.3 Cd: 7.4 Cd: 7.1 Cd: 7.5
Hg: 1.2 Hg: 1.4 Hg: 1.1 Hg: 9.13 Hg: 9.0 Hg: 10.2
24th month 6.9 As: 12.1 As: 11.6 As: 11.4 As: 121.3 As: 126.4 As: 138.4
Pb: 121.5 Pb: 123.6 Pb: 133.2 Pb: 1422.6 Pb: 1412.7 Pb: 1433.5
Cr: 159.2 Cr: 177.2 Cr: 162.1 Cr: 1522.5 Cr: 1529.3 Cr: 1511.7
Other soil indexes were tested using the method as provided in the standard GB 15618-2018 after 24 months. The results show that the soil indexes in examples 1-3 all met the requirements of land for agricultural purpose, so the land was suitable for planting other crops without hazards. The above experimental results prove that the Imperata cylindrica cultivation method of the present invention can help the Imperata cylindrica enrich metal ions in soil to reduce the content of the metal ions and remedy soil.
Claims (6)
1. An Imperata cylindrica (L.) P. Beauv. cultivation method for soil remediation, comprising the following steps of: (1) drying healthy Imperata cylindrica rhizomes at 30-40°C until the moisture is 60-80%, soaking the dried Imperata cylindrica rhizomes in a soaking solution for 24-48 h, then taking out and air-drying the Imperata cylindrica rhizomes at 20-30°C; (2) digging ditches with a depth of 5-10 cm around a mining area, planting the Imperata cylindrica rhizomes in the ditches, spraying a nutrient solution in an amount of 3 kg/mu once every half a month, and after the Imperata cylindrica grows to 15-30 cm high, spraying an ionic test solution in an amount of 4 kg/mu for six consecutive months.
2. The Imperata cylindrica cultivation method according to claim 1, wherein the soaking solution comprises polyoxyethylene, acetyl tributyl citrate and water in a mass ratio of 1:1:100.
3. The Imperata cylindrica cultivation method of claim 2, wherein a method for preparing the soaking solution comprises steps of heating a proper amount of water to 50-70°C with stirring, adding polyoxyethylene and acetyl tributyl citrate, stirring for 2-4 h, and then cooling to room temperature.
4. The Imperata cylindrica cultivation method according to claim 3, wherein the nutrient solution comprises decayed plant extract, hydroxyethyl cellulose, triethyl citrate, propylene glycol, ammonium carbonate, potassium sulfate and water in a mass ratio of 25:12:8:2:0.3:0.3:52.
5. The Imperata cylindrica cultivation method according to claim 4, wherein a method for preparing the nutrient solution comprises the following steps of: (1) heating a proper amount of water to 60-70°C, adding hydroxyethyl cellulose and triethyl citrate, vacuuming to -0.
6 Mpa to -0.8 Mpa, and then holding for 2-4 h; (2) adding propylene glycol, ammonium carbonate and potassium sulfate, stirring for 2-4 h, adding the decayed plant extract, and continuously stirring for 1-3 h.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
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| CN201910510710.1A CN110180875B (en) | 2019-06-13 | 2019-06-13 | Cultivation method of cogongrass for soil remediation |
| CN201910510710.1 | 2019-06-13 |
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| CN101861788B (en) * | 2009-04-15 | 2012-02-15 | 黄家总 | Method for fast reproducing and manufacturing lawn (greensward) |
| CN104160853A (en) * | 2014-07-21 | 2014-11-26 | 宁国千方中药发展有限公司 | Gramineous plant cogongrass rhizome planting method |
| CN104941993B (en) * | 2015-07-22 | 2017-09-12 | 句容市雾起山家庭农场 | A kind of method for comprehensive repairing in serious pollution soil |
| CN106825015A (en) * | 2017-03-13 | 2017-06-13 | 南昌工程学院 | It is a kind of that the experimental technique for repairing tungsten ore mine tailing heavy metal-polluted soil is adsorbed with cogongrass |
| CN109769612A (en) * | 2019-03-26 | 2019-05-21 | 四川好土生物科技有限公司 | A kind of thizoma curculiginis Industrialization of seeds and seedlings mating system |
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| CN110180875A (en) | 2019-08-30 |
| CN110180875B (en) | 2021-04-09 |
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