AU2018314605A1 - Cell-free protein expression using double-stranded concatameric DNA - Google Patents

Cell-free protein expression using double-stranded concatameric DNA Download PDF

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Publication number
AU2018314605A1
AU2018314605A1 AU2018314605A AU2018314605A AU2018314605A1 AU 2018314605 A1 AU2018314605 A1 AU 2018314605A1 AU 2018314605 A AU2018314605 A AU 2018314605A AU 2018314605 A AU2018314605 A AU 2018314605A AU 2018314605 A1 AU2018314605 A1 AU 2018314605A1
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AU
Australia
Prior art keywords
dna
double
sequence
nucleotide
stranded
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
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AU2018314605A
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English (en)
Inventor
Wei Gao
Erik Leeming Kvam
John Richard Nelson
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Global Life Sciences Solutions Operations UK Ltd
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Global Life Sciences Solutions Operations UK Ltd
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Publication of AU2018314605A1 publication Critical patent/AU2018314605A1/en
Assigned to Global Life Sciences Solutions Operations UK Ltd reassignment Global Life Sciences Solutions Operations UK Ltd Request for Assignment Assignors: GENERAL ELECTRIC COMPANY
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2018314605A 2017-08-11 2018-08-06 Cell-free protein expression using double-stranded concatameric DNA Pending AU2018314605A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15/631,510 US11001868B2 (en) 2017-08-11 2017-08-11 Cell-free protein expression using double-stranded concatameric DNA
US15/631,510 2017-08-11
PCT/EP2018/071229 WO2019030155A1 (en) 2017-08-11 2018-08-06 CELLULAR EXPRESSION OF PROTEINS USING DOUBLE STRANDED CONCATEMARY DNA

Publications (1)

Publication Number Publication Date
AU2018314605A1 true AU2018314605A1 (en) 2020-02-06

Family

ID=63143138

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2018314605A Pending AU2018314605A1 (en) 2017-08-11 2018-08-06 Cell-free protein expression using double-stranded concatameric DNA

Country Status (8)

Country Link
US (1) US11001868B2 (ko)
EP (1) EP3665286B1 (ko)
JP (2) JP7258361B2 (ko)
KR (1) KR102664617B1 (ko)
CN (1) CN110997922B (ko)
AU (1) AU2018314605A1 (ko)
ES (1) ES2960381T3 (ko)
WO (1) WO2019030155A1 (ko)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10077459B2 (en) 2016-05-04 2018-09-18 General Electric Company Cell-free protein expression using rolling circle amplification product
EP3818168A4 (en) * 2018-07-03 2022-04-27 Leidos, Inc. MATERIALS AND METHODS FOR CELL-FREE EXPRESSION OF VACCINE-EPITOPE-CONCATAMERS
US20200392554A1 (en) * 2019-06-13 2020-12-17 General Electric Company Expression of products from nucleic acid concatemers
WO2021084481A1 (en) 2019-10-30 2021-05-06 Progeneer, Inc. Programmed dna-driven self-assembled rna hydrogel

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE551431T1 (de) * 2001-05-14 2012-04-15 Henry Hongjun Ji Neues verfahren zur klonierung von sequenzen variabler domänen des immunologischen genrepertoires
US7405062B2 (en) 2002-05-14 2008-07-29 San Diego Antibody Method for cloning variable domain sequences of immunological gene repertoire
US20040142356A1 (en) * 2002-10-30 2004-07-22 Stacey Patterson Modified luciferase nucleic acids and methods of use
JP2005065694A (ja) * 2003-08-05 2005-03-17 Shimadzu Corp Ｄｎａ固定化方法、タンパク質合成方法、プロテインチップ作成方法及びキット
CA2563168A1 (en) * 2004-04-14 2005-11-17 President And Fellows Of Harvard College Nucleic-acid programmable protein arrays
KR20070093992A (ko) * 2004-12-11 2007-09-19 사이토제닉, 인크. 고품질 핵산의 무세포 생합성 및 그것의 사용
US7351563B2 (en) * 2005-06-10 2008-04-01 The Board Of Trustees Of The Leland Stanford Junior University Cell-free extracts and synthesis of active hydrogenase
US20080124707A1 (en) 2006-06-09 2008-05-29 Agency For Science, Technology And Research Nucleic acid concatenation
WO2009060857A1 (ja) 2007-11-05 2009-05-14 Riken 膜タンパク質の製造方法
US8715732B2 (en) 2009-01-05 2014-05-06 Cornell University Nucleic acid hydrogel via rolling circle amplification
US8871686B2 (en) * 2009-07-07 2014-10-28 Agency For Science, Technology And Research Methods of identifying a pair of binding partners
US9353393B2 (en) * 2012-02-15 2016-05-31 General Electric Company Methods and kits for reducing non-specific nucleic acid amplification
WO2014144583A2 (en) * 2013-03-15 2014-09-18 Northwestern University Methods for cell- free protein synthesis
US10011830B2 (en) 2013-05-19 2018-07-03 The Board of Trustee of theLeland Stanford junior University Devices and methods for display of encoded peptides, polypeptides, and proteins on DNA
AU2015273933B2 (en) * 2014-06-10 2021-02-11 CureVac Manufacturing GmbH Methods and means for enhancing RNA production
WO2016011226A1 (en) * 2014-07-16 2016-01-21 Moderna Therapeutics, Inc. Chimeric polynucleotides
GB201415789D0 (en) 2014-09-05 2014-10-22 Touchlight Genetics Ltd Synthesis of DNA
CN105177110A (zh) * 2015-09-11 2015-12-23 中国科学院微生物研究所 核酸的检测方法
US10077459B2 (en) 2016-05-04 2018-09-18 General Electric Company Cell-free protein expression using rolling circle amplification product
US20200392554A1 (en) * 2019-06-13 2020-12-17 General Electric Company Expression of products from nucleic acid concatemers
CN114540444B (zh) * 2022-04-23 2022-08-09 江苏申基生物科技有限公司 一种加帽组合物及其制备方法和体外转录反应体系

Also Published As

Publication number Publication date
US11001868B2 (en) 2021-05-11
JP2020533964A (ja) 2020-11-26
JP7258361B2 (ja) 2023-04-17
US20190048379A1 (en) 2019-02-14
CN110997922B (zh) 2024-04-16
JP2023052556A (ja) 2023-04-11
KR20200036925A (ko) 2020-04-07
EP3665286B1 (en) 2023-09-13
CN110997922A (zh) 2020-04-10
WO2019030155A1 (en) 2019-02-14
EP3665286A1 (en) 2020-06-17
KR102664617B1 (ko) 2024-05-10
ES2960381T3 (es) 2024-03-04

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Owner name: GLOBAL LIFE SCIENCES SOLUTIONS OPERATIONS UK LTD

Free format text: FORMER APPLICANT(S): GENERAL ELECTRIC COMPANY