AU2016297403B2 - Use of Bacillus coagulans strain in increasing egg production in laying hen - Google Patents
Use of Bacillus coagulans strain in increasing egg production in laying hen Download PDFInfo
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- 241000193749 Bacillus coagulans Species 0.000 title claims description 32
- 229940054340 bacillus coagulans Drugs 0.000 title claims description 32
- 238000004519 manufacturing process Methods 0.000 title claims description 18
- 230000001580 bacterial effect Effects 0.000 claims description 24
- 230000000813 microbial effect Effects 0.000 claims description 24
- 239000003795 chemical substances by application Substances 0.000 claims description 22
- 241000894007 species Species 0.000 claims description 18
- 241000194108 Bacillus licheniformis Species 0.000 claims description 15
- 244000063299 Bacillus subtilis Species 0.000 claims description 15
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 14
- 239000000945 filler Substances 0.000 claims description 6
- 235000021050 feed intake Nutrition 0.000 claims description 2
- 235000013601 eggs Nutrition 0.000 description 20
- 239000007788 liquid Substances 0.000 description 17
- 238000000855 fermentation Methods 0.000 description 15
- 230000004151 fermentation Effects 0.000 description 15
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 14
- 239000001963 growth medium Substances 0.000 description 14
- 239000000843 powder Substances 0.000 description 13
- 239000001888 Peptone Substances 0.000 description 9
- 108010080698 Peptones Proteins 0.000 description 9
- 239000000203 mixture Substances 0.000 description 9
- 239000006916 nutrient agar Substances 0.000 description 9
- 235000019319 peptone Nutrition 0.000 description 9
- 239000000047 product Substances 0.000 description 9
- 238000012360 testing method Methods 0.000 description 9
- 230000017448 oviposition Effects 0.000 description 8
- 241000894006 Bacteria Species 0.000 description 7
- 241000287828 Gallus gallus Species 0.000 description 7
- 239000011780 sodium chloride Substances 0.000 description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 6
- 235000015278 beef Nutrition 0.000 description 6
- 235000013330 chicken meat Nutrition 0.000 description 6
- 239000008103 glucose Substances 0.000 description 6
- 238000009395 breeding Methods 0.000 description 5
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- 244000061458 Solanum melongena Species 0.000 description 4
- 235000002597 Solanum melongena Nutrition 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 230000036750 egg laying performance Effects 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 238000012807 shake-flask culturing Methods 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 239000004575 stone Substances 0.000 description 4
- 238000009423 ventilation Methods 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 108020004465 16S ribosomal RNA Proteins 0.000 description 2
- 240000006024 Lactobacillus plantarum Species 0.000 description 2
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 235000013365 dairy product Nutrition 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 244000144992 flock Species 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 230000003862 health status Effects 0.000 description 2
- 229940072205 lactobacillus plantarum Drugs 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 235000019341 magnesium sulphate Nutrition 0.000 description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000010802 sludge Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 241000194031 Enterococcus faecium Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 244000199866 Lactobacillus casei Species 0.000 description 1
- 235000013958 Lactobacillus casei Nutrition 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003640 drug residue Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 229940017800 lactobacillus casei Drugs 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 235000013613 poultry product Nutrition 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
Abstract
Provided is a use of a
Description
USE OF BACILLUS COAGULANS STRAIN IN INCREASING EGG
PRODUCTION IN LAYING HEN
FIELD OF THE INVENTION [0001] The present invention relates to the field of microbial technology, in particular to a use of a Bacillus coagulans strain in increasing egg production at a later laying period in a laying hen.
BACKGROUND [0002] With the improvement of living standards, people are becoming increasingly concerned with food safety and healthy life problems caused by antibiotics and chemical residues, and there is an increasing demand for green poultry products without drug residues. The microbial ecological agent plays a regulating role by promoting the growth and proliferation of normal microbial floras and inhibiting the growth and proliferation of pathogenic bacteria. Due to its prominent prevention and health care effects, the microbial ecological agent can replace antibiotics and chemicals in livestock breeding and achieve true “green and pollution-free”.
[0003] Eggs have already become a necessity in people’s daily life. The use of the microbial ecological agent in laying hen breeding can improve health status of laying hens and egg production. CN 103518980B reports a microbial ecological agent obtained by mixing Lactobacillus plantarum and Bacillus subtilis, and body health level can be improved after use. CN 101715879B reports a feed additive prepared from an enzyme preparation and Enterococcus faecium, which can improve health status of chickens and egg production level. CN 103202400B reports a microbial ecological agent mixed by Lactobacillus casei, Lactobacillus plantarum and Bacillus licheniformis, which can improve the egg laying performance of broiler chickens and egg breed chickens. These reports prove that the microbial ecological agent has bright prospects in green cultivation.
[0004] Generally speaking, low-yield individuals in a chicken flock are eliminated after 300 days from egg laying, egg production after 500 days from egg laying reduces significantly, and the chicken flock is regenerated if necessary. However, there are few researches on how to improve the egg laying performance of laying hens at a later laying period and utilize feed to the maximum extent.
SUMMARY [0005] In view of the above problems in the prior art, the applicant provides a use of a Bacillus coagulans strain in increasing egg production in a laying hen. By adopting the present invention in laying hen breeding, the egg laying performance of laying hens at a later laying period can be improved significantly, that is, the egg production and the egg laying rate are improved while the feed-egg ratio is reduced.
[0006] Technical solutions of the present invention are as follows:
[0007] A use of a Bacillus coagulans strain in increasing egg production in a laying hen, the Bacillus coagulans is combined with a Bacillus subtilis (CCTCC No. M 2011443) and a Bacillus licheniformis (CCTCC No. M 2012458) to prepare a microbial ecological combined agent capable of increasing egg production in a laying hen; and the usage amounts of the above three bacterial species are respectively as follows:
[0008] viable count of bacterial species per bacterial species gram of the microbial ecological combined agent
Bacillus coagulans Ο.4χ1Ο8-2.Οχ1Ο10 cfu
Bacillus subtilis CCTCC No. M 2011443 Ο.4χ1Ο8-2.Οχ1Ο10 cfu
Bacillus licheniformis CCTCC No. M 2012458 Ο.2χ1Ο8-1.Οχ1Ο10 cfu;
[0009] the rest is filler; and [0010] the Bacillus coagulans is a Bacillus coagulans TS-ΟΟΊ, deposited in the China Center for Type Culture Collection on May 3, 2015 with a deposit number of CCTCC No. M
2015273.
[0011] The microbial ecological combined agent has an effective viable count of 1 .Ox 108-5.Ox 1010 cfu/g, and the usage amount is 0.1%-l% of the daily feed intake of the laying hen.
[0012] 1. Screening of Bacillus coagulans [0013] 2g of soil sample near a dairy farm in Mashan, Wuxi, Jiangsu is weighed. 98 mL of sterile physiological saline is added. After heat treatment for 10 min at the temperature of 80°C, the mixture is oscillated on a shaker at a temperature of 37°C and at a speed of 120 rpm for 20 min, 1 mL of the supernatant is pipetted into an LB culture medium for dilution and coating, so as to isolate bacterial strains. The genus of the bacterial species is effectively and accurately determined through such means for the strain as morphological observation, physiological & biochemical experiments, and 16S rDNA analysis.
[0014] Finally, a strain with a serial number of ZS-007 is obtained, and identified as a Bacillus coagulans.
[0015] ZS-007 is deposited in the China Center for Type Culture Collection on May 3,
2015 with a deposit number of CCTCC M 2015273.
[0016] 2. High density fermentation of the Bacillus coagulans [0017] 1) Strain activation [0018] The Bacillus coagulans strain is inoculated into a nutrient agar slant culture medium, and incubated at a temperature of 25-37 °C for 24-48 hr; then streaked on a nutrient agar eggplant bottle slant, and incubated at a temperature of 25-37 °C for 24-48 hr.
[0019] Compositions of the nutrient agar slant culture medium and the nutrient agar eggplant bottle are both: 10 g/L peptone, 3g/L beef extract, 5 g/L sodium chloride, 15-20g/L agar, with a pH of 7.2-7.4.
[0020] 2) Shake-flask culture [0021] The activated strain is inoculated into a shake-flask, and incubated with shaking for 18-30 hr.
[0022] Formulation of shake-flask culture medium: 10 g/L peptone, 3g/L beef extract, 5 g/L sodium chloride, with a pH of 7.2-7.4. 20 mL of liquid volume in 250mL shake-flask, and 50 mL of liquid volume in 500mL shake-flask.
[0023] Culture conditions: the shake-flask is rotated at a speed of 200 r/min and a temperature of 25-37 °C.
[0024] 3) Seed tank enlarged culture [0025] The strain after enlarged culture is used for later fermentation. Compositions of seed tank culture medium: 10 g/L peptone, 3 g/L beef extract, 5 g/L sodium chloride, with a pH of 7.2-7.4; the seed tank is 10-1000L in volume, with 50-80% of liquid volume.
[0026] The culture is conducted at a stirring speed of 180-250 r/min, a ventilation amount of 0.5-1.0 vvm, and a temperature of 25-37 °C for 18-30hr.
[0027] 4) Fermentor fermentation [0028] The seed liquid with an inoculum size of 0.1%-10% is inoculated into the fermentor, the fermentation culture medium is composed of 25 g/L glucose, 15 g/L peptone, 10 g/L yeast powder, 1 g/L potassium dihydrogen phosphate, and 0.5 g/L magnesium sulfate, with an initial pH of 7.0. The fermentor is l-50m in volume, with 50-80% of liquid volume.
[0029] The culture is conducted at a stirring speed of 100-200 r/min, a ventilation amount of 0.5-0.8 vvm, and a temperature of 25-37 °C for 16-48hr.
[0030] The fermentation liquid is taken for microscopic examination, when more than 90% of bacterial bodies form spores, i.e., a fermentation end-point is arrived, the final fermentation liquid is obtained.
[0031] The viable count and the spore count in the final fermentation liquid are determined with a spread plate method, and the spore rate is calculated. In particular, the spore rate is the ratio of the spore count and the viable count. The culture medium used is a nutrient agar culture medium: 10 g/L peptone, 3g/L beef extract, 5 g/L sodium chloride, 15-20g/L agar, with a pH of 7.2-7.4. The viable count in the final fermentation liquid is determined after gradient dilution; after the final fermentation liquid is taken for heat treatment for 10 min at the temperature of 80°C and then gradient dilution, the spore count is determined. The viable count in the final fermentation liquid is 1,5X1010 cfu/mL or above, the spore count is 1.4* 1010 cfu/mL or above, and the spore rate is 90.8% or above.
[0032] 3. Preparation of Bacillus coagulans dry bacterium powder [0033] Wet bacterial sludge is collected after the final fermentation liquid obtained is centrifuged, and then spray-dried to obtain the dry bacterium powder. Operating conditions are: an air inlet temperature of 210 °C and an air outlet temperature of 100 °C. The viable count in the dry bacterium powder is 8.Ox 1011 cfu/g or above.
[0034] The beneficial technical effects of the prevent invention lie in that: when the present invention is applied in laying hen breeding, the egg laying performance of laying hens at a later laying period can be improved significantly: egg production is increased by 11% or above and egg laying rate is improved by 9.2% or above while feed-egg ratio is reduced by 10% or above.
DETAILED DESCRIPTION [0035] Hereinafter, the present invention will be described in detail with reference to embodiments.
[0036] Embodiment 1 [0037] 1. Screening of Bacillus coagulans [0038] 2g of soil sample near a dairy farm in Mashan, Wuxi, Jiangsu is weighed. 98 mL of sterile physiological saline is added. After heat treatment for 10 min at the temperature of 80°C, the mixture is oscillated on a shaker at a temperature of 37°C and at a speed of 120 rpm for 20 min, 1 mL of the supernatant is pipetted into an LB culture medium for dilution and coating, so as to isolate bacterial strains. The genus of the bacterial species is effectively and accurately determined through such means for the strain as morphological observation, physiological & biochemical experiments, and 16S rDNA analysis.
[0039] Finally, a strain with a serial number of ZS-007 is obtained, and identified as a Bacillus coagulans.
[0040] ZS-007 is deposited in the China Center for Type Culture Collection on May 3,
2015 with a deposit number of CCTCC M 2015273.
β [0041] 2. 30 m fermentor preparation [0042] Strain activation: the obtained strain is inoculated into a nutrient agar slant culture medium, and incubated at a temperature of 30 °C for 24 hr; then streaked on a nutrient agar eggplant bottle slant, and incubated at a temperature of 30 °C for 24 hr. Compositions of the nutrient agar slant culture medium and the nutrient agar eggplant bottle are both: 10 g/L peptone, 3g/Lbeef extract, 5 g/L sodium chloride, 15-20g/L agar, with a pH of 7.2-7.4.
[0043] Shake-flask culture: the activated strain is inoculated into a shake-flask, and incubated with shaking for 20 hr. Formulation of shake-flask culture medium: 10 g/L peptone, 3g/L beef extract, 5 g/L sodium chloride, with a pH of 7.2-7.4. 50 mL of liquid volume in 500mL conical flask. Culture conditions: the shake-flask is rotated at a speed of 200 r/min and a temperature of 30 °C.
[0044] Seed tank enlarged culture: the seed liquid in twenty 500mL shake-flasks is collected for seed tank inoculation. Compositions of seed tank culture medium: 10 g/L peptone, 3g/L beef extract, 5 g/L sodium chloride, with a pH of 7.2-7.4. The seed tank is 500 L in volume, with 75% of liquid volume. The culture is conducted at a stirring speed of 200 r/min, a ventilation amount of 0.8 vvm, and a temperature of 30 °C for 20 hr.
β [0045] Fermentor fermentation: 30 m fermentor, with 70% of liquid volume. Compositions of the culture medium: 25 g/L glucose, 15 g/L peptone, 10 g/L yeast powder, 1 g/L potassium dihydrogen phosphate, and 0.5 g/L magnesium sulfate, with an initial pH of 7.0. The cultured seed is inoculated into the fermentor for fermentation. The culture is conducted at a stirring speed of 100 r/min, a ventilation amount of 0.6 vvm, and a temperature of 35 °C for 40-45 hr.
[0046] The viable count in the fermentation liquid is 1.99χ1Ο10 cfu/g, the spore count is 1.92χ 1010 cfu/g, and the spore rate is 96.5 %.
[0047] 3. Preparation of dry bacterium powder [0048] Wet bacterial sludge is collected by centrifugation, and then spray-dried to obtain the dry bacterium powder. Operating conditions are: an air inlet temperature of 210 °C and an air outlet temperature of 100 °C. The viable count in the dry bacterium powder is 8.32χ 1011 cfu/g.
[0049] The Bacillus coagulans prepared in Embodiment 1 is combined with a Bacillus subtilis (CCTCC No. M 2011443) and a Bacillus licheniformis (CCTCC No. M 2012458) to obtain a microbial ecological combined agent capable of increasing egg production in a laying hen; single bacterium powders of the Bacillus subtilis (CCTCC No. M 2011443) and the Bacillus licheniformis (CCTCC No. M 2012458) are commercially available from Taizhou Lvshengyuan Biotechnology Co., Ltd. The product specifications thereof are both
5.OxlOlocfu/g.
[0050] In each 500g of microbial ecological combined agent, contents of various substances are as shown in Table 1:
[0051] Table 1
| Name | Weight |
| Bacillus coagulans CCTCC No. M 2015273 | 0.4g-200g |
| Bacillus subtilis CCTCC No. M 2011443 | 0.4g-200g |
| Bacillus licheniformis CCTCC No. M 2012458 | 0.2g-100g |
| Filler (glucose, stone powder, etc.) | 499g-0g |
[0052] After conversion, contents of various microorganisms in the microbial ecological combined agent are as shown in Table 2:
[0053] Table 2
| Bacterial species | Viable count of bacterial species per gram of product, in cfu/g |
| Bacillus coagulans CCTCC No. M 2015273 | O.4xlO8-2.OxlO10 |
| Bacillus subtilis CCTCC No. M 2011443 | O.4x1O8-2.Ox1O10 |
| Bacillus licheniformis CCTCC No. M 2012458 | O.2x1O8-1.Ox1O10 |
[0054] Embodiment 2 [0055] The Bacillus coagulans prepared in Embodiment 1 is combined with a Bacillus subtilis (CCTCC No. M 2011443) and a Bacillus licheniformis (CCTCC No. M 2012458) to prepare a microbial ecological combined agent capable of increasing egg production in a laying hen, contents of various bacterial species are as shown in Table 3. The rest in per gram of product is filler (glucose, stone powder, etc.) [0056] Table 3
| Bacterial species | Viable count of bacterial species per gram of product, in cfu/g |
| Bacillus coagulans CCTCC No. M 2015273 | 0.4xl08 |
| Bacillus subtilis CCTCC No. M 2011443 | 0.4xl08 |
| Bacillus licheniformis CCTCC No. M 2012458 | 0.2xl08 |
[0057] Embodiments [0058] The Bacillus coagulans prepared in Embodiment 1 is combined with a Bacillus subtilis (CCTCC No. M 2011443) and a Bacillus licheniformis (CCTCC No. M 2012458) to prepare a microbial ecological combined agent capable of increasing egg production in a laying hen, contents of various bacterial species are as shown in Table 4. The rest in per gram of product is filler (glucose, stone powder, etc.) [0059] Table 4
| Bacterial species | Viable count of bacterial species per gram of product, in cfu/g |
| Bacillus coagulans CCTCC No. M 2015273 | 2.OxlO10 |
| Bacillus subtilis CCTCC No. M 2011443 | 2.OxlO10 |
| Bacillus licheniformis CCTCC No. M 2012458 | l.OxlO10 |
[0060] Embodiment 4 [0061] The Bacillus coagulans prepared in Embodiment 1 is combined with a Bacillus subtilis (CCTCC No. M 2011443) and a Bacillus licheniformis (CCTCC No. M 2012458) to prepare a microbial ecological combined agent capable of increasing egg production in a laying hen, contents of various bacterial species are as shown in Table 5. The rest in per gram of product is filler (glucose, stone powder, etc.) [0062] Table 5
| Bacterial species | Viable count of bacterial species per gram of product, in cfu/g |
| Bacillus coagulans CCTCC No. M 2015273 | l.OxlO9 |
| Bacillus subtilis CCTCC No. M 2011443 | l.OxlO9 |
| Bacillus licheniformis CCTCC No. M 2012458 | l.OxlO9 |
[0063] Test example:
[0064] The microbial ecological combined agent prepared in Embodiment 3 is used for feeding the laying hens. The test is carried out at an ecological pasture for laying hens in Qidong, Nantong, Jiangsu province. Test chickens are free-ranged in a shed, 500 days old after laying eggs, with an egg laying rate which is significantly lower than the level of 90% of the egg laying rate in the peak period. There are 46 chickens in each shed, with ad libitum feed and water intake, under conventional feeding and management. During the test, all groups are fed and managed in the same way, the pre-test is 7d, and the formal test is 60d. A plot of land is enclosed according to the ecological breeding method of hens, and divided into four regions with the same areas and ecological environments, each region is provided with the same food and water troughs, laying nests and henhouses, each group is fed for 20d in a 5 region by turns, and each region is thoroughly disinfected and cleaned when taking turns, so that the influence of the position effect can be eliminated. Supplementary feeding of daily ration is conducted in the morning and evening during the test with ad libitum feed and water intake. The formal test begins on January 20, 2015 and ends on March 21, 2015. Test results are as shown in Table 6:
[0065] Table 6
| Group | Blank control group (basic rations) | Add 0.1% of product without Bacillus coagulans | Add 0.1% of microbial ecological combined agent | Add 0.25% of microbial ecological combined agent |
| Egg laying rate (%) | 50.83 | 52.38 | 55.54 | 56.01 |
| Total egg weight (g) | 70070 | 73880 | 78050 | 79450 |
| Average egg weight (g) | 50.1 | 50.7 | 50.9 | 51.4 |
| Feed consumption | 354.6 | 354.6 | 354.6 | 354.6 |
| Feed-egg ratio | 5.08 | 4.79 | 4.54 | 4.46 |
[0066] As can be seen from the table above, when the microbial ecological combined agent containing Bacillus coagulans CCTCC No. M 2015273 is used, the egg laying rate of laying hens at a later laying period is improved by 9.2% or above, the total egg production is increased by 11% or above while feed-egg ratio is reduced by 10% or above.
Claims (2)
- What is claimed is:1. A use of a Bacillus coagulans strain in increasing egg production in a laying hen, characterized in that, the Bacillus coagulans is combined with a Bacillus subtilis (CCTCC No.5 M 2011443) and a Bacillus licheniformis (CCTCC No. M 2012458) to prepare a microbial ecological combined agent capable of increasing egg production in a laying hen; and the usage amounts of the above three bacterial species are respectively as follows:bacterial speciesBacillus coagulansBacillus subtilis CCTCC No. M 2011443 viable count of bacterial species per gram of the microbial ecological combined agentO.4xlO8-2.OxlO10 cfuO.4xlO8-2.OxlO10 cfuBacillus licheniformis CCTCC No. M 2012458 O.2xlO8-l.OxlO10 cfu;the rest is filler; and the Bacillus coagulans is a Bacillus coagulans ZS-007, deposited in the China Center for 10 Type Culture Collection on May 3, 2015 with a deposit number of CCTCC No. M 2015273.
- 2. The use of a Bacillus coagulans strain in increasing egg production in a laying hen according to claim 1, characterized in that, the microbial ecological combined agent has an effective viable count of 1.Οχ1Ο8-5.Οχ1Ο10 cfu/g, and the usage amount is 0.1%-l% of the daily feed intake of the laying hen.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510435777.5A CN105062916B (en) | 2015-07-22 | 2015-07-22 | Application of one bacillus coagulans on lifting laying hen egg yield |
| CN201510435777.5 | 2015-07-22 | ||
| PCT/CN2016/090897 WO2017012571A1 (en) | 2015-07-22 | 2016-07-21 | Use of bacillus coagulans strain in increasing egg production in laying hen |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| AU2016297403A1 AU2016297403A1 (en) | 2017-10-19 |
| AU2016297403A8 AU2016297403A8 (en) | 2017-10-26 |
| AU2016297403B2 true AU2016297403B2 (en) | 2018-12-20 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2016297403A Ceased AU2016297403B2 (en) | 2015-07-22 | 2016-07-21 | Use of Bacillus coagulans strain in increasing egg production in laying hen |
Country Status (3)
| Country | Link |
|---|---|
| CN (1) | CN105062916B (en) |
| AU (1) | AU2016297403B2 (en) |
| WO (1) | WO2017012571A1 (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105062916B (en) * | 2015-07-22 | 2017-12-12 | 江南大学 | Application of one bacillus coagulans on lifting laying hen egg yield |
| CN106615952A (en) * | 2016-12-27 | 2017-05-10 | 上海创博生态工程有限公司 | Soluble microbial additive capable of improving immunity of laying hens and preparation method thereof |
| BR112020014513A2 (en) * | 2018-01-24 | 2021-01-12 | OmniGen Research, L.L.C. | BACILLUS COMBINATION FOR ADMINISTRATION TO ANIMALS |
| CN109329622A (en) * | 2018-10-30 | 2019-02-15 | 长沙拜特生物科技研究所有限公司 | Microbial inoculum containing bacillus coagulans and the application for spending pig to cultivate in Ningxiang |
| CN110218688B (en) * | 2019-07-05 | 2021-01-29 | 中国农业科学院特产研究所 | Bacillus marinus LY-23, microbial inoculum and application thereof and product using same |
| CN114698729B (en) * | 2022-03-30 | 2023-01-10 | 播恩集团股份有限公司 | Microecological preparation containing 5-methyltetrahydrofolic acid and preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013029013A1 (en) * | 2011-08-24 | 2013-02-28 | Dupont Nutrition Biosciences Aps | Enzyme producing bacillus strains |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101642198A (en) * | 2009-08-25 | 2010-02-10 | 曹君平 | Biological additive pre-mixed feed special for chicken and preparation method of biological additive substance carrier thereof |
| CN103202400B (en) * | 2013-03-22 | 2014-12-31 | 内蒙古和美科盛生物技术有限公司 | Composite microecological feed additive capable of improving producing capability of broiler chicken egg breed chicken |
| CN103518980B (en) * | 2013-05-21 | 2015-01-28 | 南京农业大学 | Preparation method for microecologics for laying hen feeds and application of microecologics |
| CN104171538B (en) * | 2014-07-07 | 2016-04-13 | 辽宁禾丰牧业股份有限公司 | A kind of grain-saving type laying hen in egg laying peak period daily ration and preparation method thereof |
| CN105062916B (en) * | 2015-07-22 | 2017-12-12 | 江南大学 | Application of one bacillus coagulans on lifting laying hen egg yield |
-
2015
- 2015-07-22 CN CN201510435777.5A patent/CN105062916B/en active Active
-
2016
- 2016-07-21 AU AU2016297403A patent/AU2016297403B2/en not_active Ceased
- 2016-07-21 WO PCT/CN2016/090897 patent/WO2017012571A1/en active Application Filing
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013029013A1 (en) * | 2011-08-24 | 2013-02-28 | Dupont Nutrition Biosciences Aps | Enzyme producing bacillus strains |
Non-Patent Citations (1)
| Title |
|---|
| FU, LIZHI, "Effect of probiotics on digestive enzyme activities and immune function in chicken", Chinese Journal of Animal Science, (2007-12-31), vol. 43, no. 11, ISSN 0258-7033, page 22 and 23 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2017012571A1 (en) | 2017-01-26 |
| AU2016297403A8 (en) | 2017-10-26 |
| AU2016297403A1 (en) | 2017-10-19 |
| CN105062916B (en) | 2017-12-12 |
| CN105062916A (en) | 2015-11-18 |
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Free format text: IN VOL 31 , NO 41 , PAGE(S) 5985 UNDER THE HEADING PCT APPLICATIONS THAT HAVE ENTERED THE NATIONAL PHASE - NAME INDEX UNDER THE NAME JIANGNAN UNIVERSITY, APPLICATION NO. 2016297403, UNDER INID (54) CORRECT THE TITLE TO READ USE OF BACILLUS COAGULANS STRAIN IN INCREASING EGG PRODUCTION IN LAYING HEN |
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