AU2016258980B2 - Potency assay - Google Patents

Potency assay Download PDF

Info

Publication number
AU2016258980B2
AU2016258980B2 AU2016258980A AU2016258980A AU2016258980B2 AU 2016258980 B2 AU2016258980 B2 AU 2016258980B2 AU 2016258980 A AU2016258980 A AU 2016258980A AU 2016258980 A AU2016258980 A AU 2016258980A AU 2016258980 B2 AU2016258980 B2 AU 2016258980B2
Authority
AU
Australia
Prior art keywords
cells
mpc
culture medium
mpcs
human
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2016258980A
Other languages
English (en)
Other versions
AU2016258980A1 (en
Inventor
Fiona SEE
Paul Simmons
Colby SUIRE
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mesoblast International SARL
Original Assignee
Mesoblast International SARL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2015901605A external-priority patent/AU2015901605A0/en
Application filed by Mesoblast International SARL filed Critical Mesoblast International SARL
Publication of AU2016258980A1 publication Critical patent/AU2016258980A1/en
Application granted granted Critical
Publication of AU2016258980B2 publication Critical patent/AU2016258980B2/en
Priority to AU2022203140A priority Critical patent/AU2022203140B2/en
Priority to AU2025205043A priority patent/AU2025205043A1/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5073—Stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/04—Drugs for skeletal disorders for non-specific disorders of the connective tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/475—Assays involving growth factors
    • G01N2333/495—Transforming growth factor [TGF]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/10—Musculoskeletal or connective tissue disorders

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Urology & Nephrology (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Epidemiology (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Rheumatology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physical Education & Sports Medicine (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
AU2016258980A 2015-05-05 2016-05-04 Potency assay Active AU2016258980B2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2022203140A AU2022203140B2 (en) 2015-05-05 2022-05-11 Potency assay
AU2025205043A AU2025205043A1 (en) 2015-05-05 2025-07-02 Potency assay

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AU2015901605 2015-05-05
AU2015901605A AU2015901605A0 (en) 2015-05-05 Potency assay
PCT/EP2016/060049 WO2016177805A1 (en) 2015-05-05 2016-05-04 Potency assay

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2022203140A Division AU2022203140B2 (en) 2015-05-05 2022-05-11 Potency assay

Publications (2)

Publication Number Publication Date
AU2016258980A1 AU2016258980A1 (en) 2017-11-23
AU2016258980B2 true AU2016258980B2 (en) 2022-02-17

Family

ID=56087237

Family Applications (3)

Application Number Title Priority Date Filing Date
AU2016258980A Active AU2016258980B2 (en) 2015-05-05 2016-05-04 Potency assay
AU2022203140A Active AU2022203140B2 (en) 2015-05-05 2022-05-11 Potency assay
AU2025205043A Pending AU2025205043A1 (en) 2015-05-05 2025-07-02 Potency assay

Family Applications After (2)

Application Number Title Priority Date Filing Date
AU2022203140A Active AU2022203140B2 (en) 2015-05-05 2022-05-11 Potency assay
AU2025205043A Pending AU2025205043A1 (en) 2015-05-05 2025-07-02 Potency assay

Country Status (9)

Country Link
US (3) US11795435B2 (OSRAM)
EP (2) EP3292199B1 (OSRAM)
JP (3) JP6797833B2 (OSRAM)
KR (2) KR102885856B1 (OSRAM)
CN (2) CN115141872A (OSRAM)
AU (3) AU2016258980B2 (OSRAM)
CA (1) CA2984987C (OSRAM)
ES (2) ES2911266T3 (OSRAM)
WO (1) WO2016177805A1 (OSRAM)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3714038A1 (en) 2017-11-22 2020-09-30 Mesoblast International Sàrl Cellular compositions and methods of treatment i
KR102506822B1 (ko) * 2018-09-20 2023-03-06 시토스핀, 에스.엘. 기능적 중간엽 줄기 세포의 농축된 집단을 수득하는 방법, 이의 수득된 세포 및 이를 포함하는 조성물
JP2022515916A (ja) * 2019-01-02 2022-02-22 メゾブラスト・インターナショナル・エスアーエールエル 腰痛を治療するための方法
CN115605754A (zh) * 2020-04-20 2023-01-13 美商生命科学有限公司（Us） 效价测定
CN120418656A (zh) * 2022-12-23 2025-08-01 康干细胞生物科技有限公司 用于选择具有增强的软骨分化能力的干细胞的方法及包括其的细胞疗法产品

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3089815A (en) 1951-10-11 1963-05-14 Lieb Hans Injectable pharmaceutical preparation, and a method of making same
US4593089A (en) 1980-07-30 1986-06-03 Abbott Laboratories Fluorescent polarization immunoassay utilizing substituted triazinylaminofluorescein aminoglycosides
US4751190A (en) 1985-07-22 1988-06-14 Abbott Laboratories Fluorescence polarization immunoassay and reagents for use therein
US5139941A (en) 1985-10-31 1992-08-18 University Of Florida Research Foundation, Inc. AAV transduction vectors
US4983393A (en) 1987-07-21 1991-01-08 Maximed Corporation Intra-vaginal device and method for sustained drug release
AU8200191A (en) 1990-07-09 1992-02-04 United States of America, as represented by the Secretary, U.S. Department of Commerce, The High efficiency packaging of mutant adeno-associated virus using amber suppressions
US5173414A (en) 1990-10-30 1992-12-22 Applied Immune Sciences, Inc. Production of recombinant adeno-associated virus vectors
US5486359A (en) 1990-11-16 1996-01-23 Osiris Therapeutics, Inc. Human mesenchymal stem cells
DK0648271T3 (da) 1991-08-20 2003-07-21 Us Gov Health & Human Serv Adenovirusmedieret overførsel af gener til mave-/tarmkanal
DE4211351A1 (de) 1992-04-04 1993-10-07 Behringwerke Ag Verfahren zur Analyse partikelverstärkter Agglutinationsreaktionen auf Zentrifugalanalysatoren durch Bestimmung der Trübungsaufhellung
JPH08510134A (ja) 1993-05-17 1996-10-29 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア Ｈｉｖ感染およびエイズを対象としたリボザイム遺伝子治療
US6541213B1 (en) 1996-03-29 2003-04-01 University Of Washington Microscale diffusion immunoassay
CA2244326C (en) 1997-08-11 2006-03-28 Shinichi Eda Microparticle enhanced light scattering agglutination assay and microparticle reagents therefor
WO2000042430A1 (en) 1999-01-15 2000-07-20 Medtox Scientific, Inc. Lateral flow test strip
AU2003901668A0 (en) 2003-03-28 2003-05-01 Medvet Science Pty. Ltd. Non-haemopoietic precursor cells
CA2413337C (en) 2000-06-22 2009-11-24 Thomas E. Davis Bioadhesive compositions and methods of preparation and use
EP1419387B1 (en) 2001-08-20 2012-01-04 Proteome Systems Ltd. Diagnostic testing process
US20040265926A1 (en) 2002-11-21 2004-12-30 Leong Ng Bodily fluid markers of tissue hypoxia
KR101446634B1 (ko) 2005-04-12 2014-10-16 메소블라스트, 아이엔씨. 조직 비특이적인 알카리 포스파타제에 의한 다분화성 성체세포의 분리
KR100802011B1 (ko) 2005-08-10 2008-02-12 인하대학교 산학협력단 층분리배양법을 이용한 골수에서의 중간엽 줄기세포분리방법
JP2011196840A (ja) * 2010-03-19 2011-10-06 Univ Of Tokyo 急性大動脈解離の検査方法
US20140286916A1 (en) * 2011-10-11 2014-09-25 Bone Therapeutics Uses of Growth and Differentiation Factor 8 (GDF-8)
NZ715203A (en) * 2013-05-16 2019-07-26 Agency Science Tech & Res Heparan sulphates
KR20150016117A (ko) 2013-07-30 2015-02-11 코아스템(주) 인간 골수 유래 중간엽 줄기세포를 유효성분으로 포함하는 자가면역질환의 예방 또는 치료용 약제학적 조성물
CN103990181B (zh) * 2014-05-26 2015-07-08 中国人民解放军第四军医大学 一种具有诱导活性微载体-细胞复合物的制备方法及其应用

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHENG CAO ET AL, "Bone marrow mesenchymal stem cells slow intervertebral disc degeneration through the NF-[kappa]B pathway", THE SPINE JOURNAL, AMSTERDAM, NL, (2015-03-01), vol. 15, no. 3, pages 530 - 538 *
DOBROSLAV KYURKCHIEV, "Secretion of immunoregulatory cytokines by mesenchymal stem cells", WORLD JOURNAL OF STEM CELLS, (2014-01-01), vol. 6, no. 5, doi:10.4252/wjsc.v6.i5.552, ISSN 1948-0210, page 552 *
LI WEN ET AL, "Immunomodulatory Effects of Bone Marrow-Derived Mesenchymal Stem Cells on Pro-Inflammatory Cytokine-Stimulated Human Corneal Epithelial Cells", PLOS ONE, (2014-07-08), vol. 9, no. 7, page e101841 *

Also Published As

Publication number Publication date
BR112017023660A2 (pt) 2018-07-17
CN107771220A (zh) 2018-03-06
AU2016258980A1 (en) 2017-11-23
US20240076621A1 (en) 2024-03-07
JP7754893B2 (ja) 2025-10-15
CN115141872A (zh) 2022-10-04
ES2911266T3 (es) 2022-05-18
US20200140822A1 (en) 2020-05-07
JP2018517900A (ja) 2018-07-05
EP3292199B1 (en) 2022-01-12
JP2023159260A (ja) 2023-10-31
KR102885856B1 (ko) 2025-11-14
EP4015624A1 (en) 2022-06-22
CA2984987C (en) 2023-10-10
AU2022203140A1 (en) 2022-06-02
CA2984987A1 (en) 2016-11-10
HK1247236A1 (zh) 2018-09-21
KR102582602B1 (ko) 2023-09-25
US11795435B2 (en) 2023-10-24
JP7335222B2 (ja) 2023-08-29
EP4015624B1 (en) 2025-02-19
KR20230140604A (ko) 2023-10-06
CN107771220B (zh) 2022-07-05
AU2025205043A1 (en) 2025-07-24
WO2016177805A1 (en) 2016-11-10
JP2021036892A (ja) 2021-03-11
EP3292199A1 (en) 2018-03-14
AU2022203140B2 (en) 2025-04-10
KR20180041621A (ko) 2018-04-24
ES3018944T3 (en) 2025-05-19
JP6797833B2 (ja) 2020-12-09
US20240409898A1 (en) 2024-12-12

Similar Documents

Publication Publication Date Title
AU2022203140B2 (en) Potency assay
Mendelson et al. Chondrogenesis by chemotactic homing of synovium, bone marrow, and adipose stem cells in vitro
Sesia et al. Anti‐Inflammatory/tissue repair macrophages enhance the cartilage‐forming capacity of human bone marrow‐derived mesenchymal stromal cells
US20220143097A1 (en) Method for treating lower back pain
Simon et al. The effects of hyperacute serum on the elements of the human subchondral bone marrow niche
Altaie et al. Platelet lysate enhances synovial fluid multipotential stromal cells functions: Implications for therapeutic use
Bertolo et al. Comparative characterization of canine and human mesenchymal stem cells derived from bone marrow
HK1247236B (en) Potency assay
Ghavimi et al. Human cartilage progenitor cells from ear, nose, rib, and joint have a robust, stable phenotype for cartilage repair
BR112017023660B1 (pt) Método para selecionar células precursoras de linhagem mesenquimal ou células-tronco humanas, composições e uso das mesmas
Hofer Traumatized muscle-derived multipotent progenitor cells: Pro-angiogenic activity, promotion of nerve growth, and osteogenic differentiation
Beaumont Dental pulp stem cells and leukocyte-and platelet-rich fibrin as candidate therapies for articular cartilage and tendon repair

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)