AU2015220911B2 - Covalently closed non-coding immunomodulatory DNA construct - Google Patents

Covalently closed non-coding immunomodulatory DNA construct Download PDF

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Publication number
AU2015220911B2
AU2015220911B2 AU2015220911A AU2015220911A AU2015220911B2 AU 2015220911 B2 AU2015220911 B2 AU 2015220911B2 AU 2015220911 A AU2015220911 A AU 2015220911A AU 2015220911 A AU2015220911 A AU 2015220911A AU 2015220911 B2 AU2015220911 B2 AU 2015220911B2
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Prior art keywords
dna
dna construct
construct
vaccine
pharmaceutical composition
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Ceased
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AU2015220911A
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AU2015220911A1 (en
Inventor
Kerstin KAPP
Christiane Kleuss
Matthias Schroff
Burghardt Wittig
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Mologen AG
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Mologen AG
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/117Nucleic acids having immunomodulatory properties, e.g. containing CpG-motifs
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804Nucleic acid analysis using immunogens
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/70Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088Compounds having three or more nucleosides or nucleotides
    • A61K31/713Double-stranded nucleic acids or oligonucleotides
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/39Medicinal preparations containing antigens or antibodies characterised by the immunostimulating additives, e.g. chemical adjuvants
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00Drugs for disorders of the respiratory system
    • A61P11/06Antiasthmatics
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00Antineoplastic agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00Drugs for immunological or allergic disorders
    • A61P37/02Immunomodulators
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00Drugs for immunological or allergic disorders
    • A61P37/08Antiallergic agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K2039/555Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
    • A61K2039/55511Organic adjuvants
    • A61K2039/55561CpG containing adjuvants; Oligonucleotide containing adjuvants
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/17Immunomodulatory nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/50Physical structure
    • C12N2310/53Physical structure partially self-complementary or closed
    • C12N2310/532Closed or circular
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00Applications; Uses
    • C12N2320/30Special therapeutic applications
    • C12N2320/31Combination therapy

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Epidemiology (AREA)
  • Plant Pathology (AREA)
  • Pulmonology (AREA)
  • Mycology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Pathology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicinal Preparation (AREA)
AU2015220911A 2014-02-18 2015-02-18 Covalently closed non-coding immunomodulatory DNA construct Ceased AU2015220911B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1402847.6 2014-02-18
GB1402847.6A GB2523187A (en) 2014-02-18 2014-02-18 Covalently closed non-coding immunomodulatory DNA construct
PCT/EP2015/053396 WO2015124614A1 (en) 2014-02-18 2015-02-18 Covalently closed non-coding immunomodulatory dna construct

Publications (2)

Publication Number Publication Date
AU2015220911A1 AU2015220911A1 (en) 2015-10-15
AU2015220911B2 true AU2015220911B2 (en) 2017-02-02

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ID=50440342

Family Applications (1)

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AU2015220911A Ceased AU2015220911B2 (en) 2014-02-18 2015-02-18 Covalently closed non-coding immunomodulatory DNA construct

Country Status (16)

Country Link
US (1) US10280424B2 (es)
EP (1) EP2999787B1 (es)
JP (1) JP6067893B2 (es)
KR (1) KR101831713B1 (es)
CN (1) CN105264074A (es)
AU (1) AU2015220911B2 (es)
BR (1) BR112015025748A2 (es)
CA (1) CA2908522A1 (es)
DK (1) DK2999787T3 (es)
ES (1) ES2634002T3 (es)
GB (1) GB2523187A (es)
HK (1) HK1216758A1 (es)
MX (1) MX2015014190A (es)
RU (1) RU2668387C2 (es)
SG (1) SG11201508108YA (es)
WO (1) WO2015124614A1 (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105579582A (zh) 2013-07-25 2016-05-11 埃克西奎雷股份有限公司 用于预防和治疗用途的作为免疫刺激剂的基于球形核酸的构建体
US10568898B2 (en) 2013-08-13 2020-02-25 Northwestern University Lipophilic nanoparticles for drug delivery
GB2523187A (en) * 2014-02-18 2015-08-19 Mologen Ag Covalently closed non-coding immunomodulatory DNA construct
EP3164113B1 (en) 2014-06-04 2019-03-27 Exicure, Inc. Multivalent delivery of immune modulators by liposomal spherical nucleic acids for prophylactic or therapeutic applications
JP2017537619A (ja) 2014-11-21 2017-12-21 ノースウェスタン ユニバーシティ 球状核酸ナノ粒子複合体の配列特異的細胞内取込
LU92821B1 (en) * 2015-09-09 2017-03-20 Mologen Ag Combination comprising immunostimulatory oligonucleotides
GB2542425A (en) 2015-09-21 2017-03-22 Mologen Ag Means for the treatment of HIV
US11364304B2 (en) 2016-08-25 2022-06-21 Northwestern University Crosslinked micellar spherical nucleic acids
WO2018209270A1 (en) 2017-05-11 2018-11-15 Northwestern University Adoptive cell therapy using spherical nucleic acids (snas)
SG11202001683SA (en) 2017-08-31 2020-03-30 Mologen Ag Tlr-9 agonists for modulation of tumor microenvironment
AU2021213927A1 (en) * 2020-01-31 2022-07-28 Tyris Therapeutics, S.L. Closed linear DNA with modified nucleotides

Citations (1)

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Publication number Priority date Publication date Assignee Title
US20090053250A1 (en) * 2004-08-09 2009-02-26 Mologen Ag Immune Modulating Oligonucleotides in Connection with Chemotherapeutic Measures

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US20030050263A1 (en) * 1994-07-15 2003-03-13 The University Of Iowa Research Foundation Methods and products for treating HIV infection
US6207646B1 (en) 1994-07-15 2001-03-27 University Of Iowa Research Foundation Immunostimulatory nucleic acid molecules
DE19935756A1 (de) 1999-07-27 2001-02-08 Mologen Forschungs Entwicklung Kovalent geschlossenes Nukleinsäuremolekül zur Immunstimulation
US20040101534A1 (en) * 2002-06-25 2004-05-27 Diamond Don J. Adjuvant-free peptide vaccine
KR20050115913A (ko) * 2003-03-26 2005-12-08 사이토스 바이오테크놀로지 아게 Melan―a 펩티드 유사체-바이러스-양-입자컨쥬게이트
SG122973A1 (en) 2003-10-30 2006-06-29 Coley Pharm Gmbh C-class oligonucleotide analogs with enhanced immunostimulatory potency
ATE512668T1 (de) * 2003-12-30 2011-07-15 Mologen Ag Allogenes tumortherapeutikum
EP1716234B1 (de) * 2004-02-20 2013-10-02 Mologen AG Substituiertes, nicht-kodierendes nukleinsäuremolekül zur therapeutischen und prophylaktischen immunstimulation in menschen und höheren tieren
BRPI0711607A2 (pt) * 2006-05-11 2012-11-06 Mologen Ag molécula multimérica para modulação da atividade do sistema imunológico humano ou animal, agente de combinação, kit, agente farmacêutico, molécula e uso da mesma
WO2010039137A1 (en) 2008-10-02 2010-04-08 Idera Pharmaceuticals, Inc. Immune regulatory oligonucleotide (iro) compounds to modulate toll-like receptor based immune response
EP2246433A1 (de) 2009-04-30 2010-11-03 Mologen AG Concatemere zur Immunmodulation
GB2523187A (en) * 2014-02-18 2015-08-19 Mologen Ag Covalently closed non-coding immunomodulatory DNA construct

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US20090053250A1 (en) * 2004-08-09 2009-02-26 Mologen Ag Immune Modulating Oligonucleotides in Connection with Chemotherapeutic Measures

Non-Patent Citations (2)

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Kreig AM, Nature Reviews Drug Discovery, 2006, 5:471-484 *
Schmidt M et al, Allergy, 2006, 61:56-63 *

Also Published As

Publication number Publication date
EP2999787A1 (en) 2016-03-30
SG11201508108YA (en) 2015-10-29
US20160348114A1 (en) 2016-12-01
KR20150130425A (ko) 2015-11-23
KR101831713B1 (ko) 2018-02-23
MX2015014190A (es) 2015-12-11
US10280424B2 (en) 2019-05-07
HK1216758A1 (zh) 2016-12-02
EP2999787B1 (en) 2017-04-19
WO2015124614A1 (en) 2015-08-27
GB201402847D0 (en) 2014-04-02
CA2908522A1 (en) 2015-08-27
AU2015220911A1 (en) 2015-10-15
JP6067893B2 (ja) 2017-01-25
RU2015140720A (ru) 2017-03-29
RU2668387C2 (ru) 2018-09-28
JP2016526373A (ja) 2016-09-05
BR112015025748A2 (pt) 2017-10-10
GB2523187A (en) 2015-08-19
DK2999787T3 (en) 2017-08-07
ES2634002T3 (es) 2017-09-26
CN105264074A (zh) 2016-01-20

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