AU2014318839B2 - Compositions and methods for the delivery of molecules into live cells - Google Patents

Compositions and methods for the delivery of molecules into live cells Download PDF

Info

Publication number
AU2014318839B2
AU2014318839B2 AU2014318839A AU2014318839A AU2014318839B2 AU 2014318839 B2 AU2014318839 B2 AU 2014318839B2 AU 2014318839 A AU2014318839 A AU 2014318839A AU 2014318839 A AU2014318839 A AU 2014318839A AU 2014318839 B2 AU2014318839 B2 AU 2014318839B2
Authority
AU
Australia
Prior art keywords
cell
cells
dtat
compound
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2014318839A
Other languages
English (en)
Other versions
AU2014318839A1 (en
Inventor
Jean-Philippe PELLOIS
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Texas A&M University System
Original Assignee
Texas A&M University System
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Texas A&M University System filed Critical Texas A&M University System
Publication of AU2014318839A1 publication Critical patent/AU2014318839A1/en
Application granted granted Critical
Publication of AU2014318839B2 publication Critical patent/AU2014318839B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
    • A61K47/64—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
    • A61K47/645—Polycationic or polyanionic oligopeptides, polypeptides or polyamino acids, e.g. polylysine, polyarginine, polyglutamic acid or peptide TAT

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
AU2014318839A 2013-09-10 2014-09-10 Compositions and methods for the delivery of molecules into live cells Active AU2014318839B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361876006P 2013-09-10 2013-09-10
US61/876,006 2013-09-10
PCT/US2014/055021 WO2015038662A1 (en) 2013-09-10 2014-09-10 Compositions and methods for the delivery of molecules into live cells

Publications (2)

Publication Number Publication Date
AU2014318839A1 AU2014318839A1 (en) 2016-03-31
AU2014318839B2 true AU2014318839B2 (en) 2019-12-05

Family

ID=52666235

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2014318839A Active AU2014318839B2 (en) 2013-09-10 2014-09-10 Compositions and methods for the delivery of molecules into live cells

Country Status (7)

Country Link
US (2) US9662404B2 (cg-RX-API-DMAC10.html)
EP (1) EP3043811B1 (cg-RX-API-DMAC10.html)
JP (1) JP6659546B2 (cg-RX-API-DMAC10.html)
AU (1) AU2014318839B2 (cg-RX-API-DMAC10.html)
CA (1) CA2923664C (cg-RX-API-DMAC10.html)
ES (1) ES2794599T3 (cg-RX-API-DMAC10.html)
WO (1) WO2015038662A1 (cg-RX-API-DMAC10.html)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3149413C (en) * 2015-04-10 2025-07-08 Feldan Bio Inc. Polypeptide-based carrier agents to improve the transduction efficiency of polypeptide cargo to cytosol in target eukaryotic cells and their uses, methods, and related kits
FR3048698B1 (fr) 2016-03-11 2021-03-05 Univ Claude Bernard Lyon Inhibiteurs de l'interaction bcl2 l10 / recepteur ip3
US11629170B2 (en) 2016-10-12 2023-04-18 Feldan Bio Inc. Rationally-designed synthetic peptide shuttle agents for delivering polypeptide cargos from an extracellular space to the cytosol and/or nucleus of a target eukaryotic cell, uses thereof, methods and kits relating to same
AU2017341736B2 (en) * 2016-10-12 2022-09-08 Feldan Bio Inc. Rationally-designed synthetic peptide shuttle agents for delivering polypeptide cargos from an extracellular space to the cytosol and/or nucleus of a target eukaryotic cell, uses thereof, methods and kits relating to same
JP7068711B2 (ja) * 2017-03-22 2022-05-17 国立大学法人京都大学 細胞質送達ペプチド
WO2019036375A1 (en) 2017-08-14 2019-02-21 Sanford Burnham Prebys Medical Discovery Institute CARDIOGENIC MESODERMA TRAINING REGULATORS
US12029804B2 (en) 2018-11-02 2024-07-09 Soonchunhyang University Industry Academy Cooperation Foundation Peptide for promoting mucous membrane permeation and composition containing same
CN113423834A (zh) 2018-11-08 2021-09-21 加总生物公司 微核小体核心蛋白及其在核酸递送中的用途
KR102201154B1 (ko) * 2019-03-26 2021-01-12 가천대학교 산학협력단 폴리글루타메이트-TAT-Cre 융합 단백질의 제조방법
CA3040645A1 (en) 2019-04-18 2020-10-18 Feldan Bio Inc. Peptide-based non-proteinaceous cargo delivery
EP4136235A4 (en) 2020-04-13 2024-10-16 Summation Bio, Inc. MINI-NUCLEOSOME CORE PROETIN AND THEIR USE IN NUCLEIC ACID DELIVERY

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999047173A2 (en) * 1998-03-19 1999-09-23 University Of Medicine And Dentistry Of New Jersey Carrier for in vivo delivery of a therapeutic agent
WO2009067757A1 (en) * 2007-11-30 2009-06-04 Cytomatrix Pty Ltd Methods of inducing pluripotency involving oct4 protein
WO2010115052A2 (en) * 2009-04-03 2010-10-07 The Mclean Hospital Corporation Induced pluripotent stem cells

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1968643A2 (en) 2005-12-16 2008-09-17 Diatos Cell penetrating peptide conjugates for delivering of nucleic acids into a cell
WO2010059806A2 (en) * 2008-11-20 2010-05-27 Cedars-Sinai Medical Center Generation of induced pluripotent stem cells without the use of viral vectors
WO2010072228A1 (en) * 2008-12-22 2010-07-01 Xigen S.A. Novel transporter constructs and transporter cargo conjugate molecules
CA3032186C (en) 2010-07-13 2022-04-26 Council on Postsecondary Education, State of Rhode Island and Providence Plantations Environmentally sensitive composition comprising a ph triggered peptide and uses thereof
WO2012090150A2 (en) 2010-12-27 2012-07-05 Compugen Ltd New cell-penetrating peptides and uses thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999047173A2 (en) * 1998-03-19 1999-09-23 University Of Medicine And Dentistry Of New Jersey Carrier for in vivo delivery of a therapeutic agent
WO2009067757A1 (en) * 2007-11-30 2009-06-04 Cytomatrix Pty Ltd Methods of inducing pluripotency involving oct4 protein
WO2010115052A2 (en) * 2009-04-03 2010-10-07 The Mclean Hospital Corporation Induced pluripotent stem cells

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ARCHANA CHUGH ET AL: "Translocation of cell-penetrating peptides and delivery of their cargoes in triticale microspores", PLANT CELL REPORTS, vol. 28, no. 5, 15 March 2009 (2009-03-15), pages 801-810 *
CARSTEN RUDOLPH ET AL: PHARMACEUTICAL RESEARCH, vol. 21, no. 9, 1September2004 (2004-09-01), pages 1662-1669 *
KROSL J ET AL: "In vitro expansion of hematopoietic stem cells by recombinant TAT-HOXB4 protein", NATURE MEDICINE, vol. 9, no. 11, 1 November 2003 (2003-11-01 ), pages 1428-1432 *
LEE SOO-JIN ET AL: "Enhancement of gene delivery using novel homodimeric tat peptide formed by disulfide bond.", JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY AUG 2011, vol. 21, no. 8, August 2011 (2011-08), pages 802-807 *
WAN LI ET AL: AIDS RESEARCH AND THERAPY, vol. 3, no. 1, 24 April 2006 (2006-04-24), page 12 *

Also Published As

Publication number Publication date
JP2016530303A (ja) 2016-09-29
JP6659546B2 (ja) 2020-03-04
CA2923664A1 (en) 2015-03-19
US9662404B2 (en) 2017-05-30
EP3043811A4 (en) 2017-09-20
WO2015038662A1 (en) 2015-03-19
EP3043811A1 (en) 2016-07-20
EP3043811B1 (en) 2020-03-25
US20170258933A1 (en) 2017-09-14
US20150099690A1 (en) 2015-04-09
ES2794599T3 (es) 2020-11-18
CA2923664C (en) 2023-05-02

Similar Documents

Publication Publication Date Title
EP3043811B1 (en) Compositions and methods for the delivery of molecules into live cells
AU2014318839A1 (en) Compositions and methods for the delivery of molecules into live cells
Lundin et al. Distinct uptake routes of cell-penetrating peptide conjugates
Takayama et al. Enhanced intracellular delivery using arginine-rich peptides by the addition of penetration accelerating sequences (Pas)
EP1991560B1 (en) Peptide having cell membrane penetrating activity
Mäger et al. Assessing the uptake kinetics and internalization mechanisms of cell-penetrating peptides using a quenched fluorescence assay
Buyanova et al. Discovery of a cyclic cell-penetrating peptide with improved endosomal escape and cytosolic delivery efficiency
AU2508501A (en) Polypeptides comprising multimers of nuclear localization signals or of protein transduction domains and their use for transferring molecules into cells
CN102811744A (zh) 用于改善基因调节化合物的递送的化学修饰的细胞渗透性肽
US20200207834A1 (en) Gene transfer systems for stem cell engineering
KR101695792B1 (ko) 신규 세포막 투과 펩티드 및 생리활성 물질 전달체로서의 그의 용도
US8889631B2 (en) Disruptors of early/recycling endosomes
WO2012113846A1 (en) A system for cargo delivery into the cells
Kubi et al. Cell-penetrating and mitochondrion-targeting molecules
US20250269046A1 (en) Cellular uptake of large biomolecules enabled by cell-surface-reactive cell-penetrating peptide additives
GB2357084A (en) A hydrophobic carrier peptide
KR20090122769A (ko) 세포내로 단백질을 전달하는 방법 및 이를 위한 펩타이드
Najjar et al. An L-to D-amino acid conversion in the cell penetrating peptide dfTAT influences proteolytic stability, endocytic uptake, and endosomal escape
Yokoo Peptide and Protein Delivery
Najjar Identifying and Characterizing Molecular Parameters that Modulate the Endosomal Escape of Cationic Cell-Penetrating Peptides: A Structure Activity Approach
Smith Modified folates for selective delivery into cancer cells
Hofmann Conditional Cell Penetration of a Masked CPP and Optimization of Multimeric CPP-Delivery Modules for Intracellular Cargo Delivery
Joseph Poly (Arginine) Derived Cancer-Targeting Peptides for the Development of a Cancer-Targeted Gene Therapy Approach in HepG2 Liver Cancer Cells
Erazo Cytosolic Delivery of Proteins, Peptides and Cell-Impermeable Small Molecules Into Live Cells Utilizing Virus-Inspired Multivalent Cell-Penetrating Peptides: Principles and Mechanisms
Jiang et al. 31 Design and Synthesis of Cell-Penetrating Peptides

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)