AU2014227754B2 - Encoded nanopore sensor for multiplex nucleic acids detection - Google Patents

Encoded nanopore sensor for multiplex nucleic acids detection Download PDF

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Publication number
AU2014227754B2
AU2014227754B2 AU2014227754A AU2014227754A AU2014227754B2 AU 2014227754 B2 AU2014227754 B2 AU 2014227754B2 AU 2014227754 A AU2014227754 A AU 2014227754A AU 2014227754 A AU2014227754 A AU 2014227754A AU 2014227754 B2 AU2014227754 B2 AU 2014227754B2
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probe
nucleic acid
target nucleic
probe molecule
nanopore
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AU2014227754A1 (en
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Li-qun GU
Xinyue ZHANG
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University of Missouri St Louis
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University of Missouri Columbia
University of Missouri St Louis
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Nanotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU2014227754A 2013-03-15 2014-03-14 Encoded nanopore sensor for multiplex nucleic acids detection Active AU2014227754B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361788589P 2013-03-15 2013-03-15
US61/788,589 2013-03-15
PCT/US2014/028528 WO2014144217A1 (en) 2013-03-15 2014-03-14 Encoded nanopore sensor for multiplex nucleic acids detection

Publications (2)

Publication Number Publication Date
AU2014227754A1 AU2014227754A1 (en) 2015-10-01
AU2014227754B2 true AU2014227754B2 (en) 2018-03-01

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AU2014227754A Active AU2014227754B2 (en) 2013-03-15 2014-03-14 Encoded nanopore sensor for multiplex nucleic acids detection

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US (2) US9732379B2 (https=)
EP (1) EP2971118B1 (https=)
JP (1) JP2016517275A (https=)
AU (1) AU2014227754B2 (https=)
CA (1) CA2906007C (https=)
WO (1) WO2014144217A1 (https=)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10732183B2 (en) * 2013-03-15 2020-08-04 The Trustees Of Columbia University In The City Of New York Method for detecting multiple predetermined compounds in a sample
EP3253889A4 (en) * 2015-02-02 2018-09-26 Two Pore Guys, Inc. Nanopore detection of target polynucleotides from sample background
CA2986921A1 (en) 2015-06-02 2016-12-08 Nanopore Diagnostics, Llc Nucleic acid detection
WO2018093976A1 (en) 2016-11-16 2018-05-24 Nanopore Diagnostics, Llc Analysis of nucleic acids using probe with non-linear tag
US11034996B2 (en) 2018-03-02 2021-06-15 The Curators Of The University Of Missouri Nucleic acid sequence and capture by formation of an abasic site-derived cross-link
JP7377222B2 (ja) * 2018-06-21 2023-11-09 エフ． ホフマン－ラ ロシュ アーゲー 配列決定のためのトンネル接合部
JP7239965B2 (ja) * 2018-06-26 2023-03-15 国立大学法人東京農工大学 ２種以上の特定オリゴヌクレオチドの有無を判定する判定方法、並びに、前記判定方法を用いて検査対象が小細胞肺がん、胆管がんに罹患している又はそのリスクを有することを判定する方法
JP7344786B2 (ja) * 2019-12-19 2023-09-14 株式会社日立製作所 溶液中の任意のｄｎａ配列を同定する方法
CN112795628B (zh) * 2021-02-05 2024-02-23 南京邮电大学 基于固态纳米孔传感器的miRNA检测方法、检测探针及试剂盒

Family Cites Families (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL86164A0 (en) * 1987-04-28 1988-11-15 Tamir Biotechnology Ltd Improved dna probes
EP0304845A3 (en) * 1987-08-28 1991-03-06 Profile Diagnostic Sciences Inc. Method and kit for assaying gene expressions
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US20040152084A1 (en) 2003-01-31 2004-08-05 Slattum Paul M. Compounds and processes for single-pot attachment of a label to nucleic acid
WO1999005167A1 (en) 1997-07-25 1999-02-04 University Of Massachusetts Designed protein pores as components for biosensors
US6395486B1 (en) * 1999-03-15 2002-05-28 Applera Corporation Probe/mobility modifier complexes for multiplexnucleic acid detection
US6537755B1 (en) * 1999-03-25 2003-03-25 Radoje T. Drmanac Solution-based methods and materials for sequence analysis by hybridization
US6428959B1 (en) 1999-09-07 2002-08-06 The Regents Of The University Of California Methods of determining the presence of double stranded nucleic acids in a sample
WO2001059453A2 (en) * 2000-02-11 2001-08-16 The Texas A & M University System Biosensor compositions and methods of use
WO2003000920A2 (en) 2001-06-21 2003-01-03 President And Fellows Of Harvard College Methods for characterization of nucleic acid molecules
AU2002365421A1 (en) 2001-07-10 2003-09-02 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for detecting the activation state of the multiple proteins in single cells
AU2002362013B2 (en) * 2001-11-21 2008-04-24 Applied Biosystems, Llc. Digital assay
US20050019784A1 (en) 2002-05-20 2005-01-27 Xing Su Method and apparatus for nucleic acid sequencing and identification
AU2003240482B2 (en) 2002-05-30 2009-03-12 The Scripps Research Institute Copper-catalysed ligation of azides and acetylenes
US7683036B2 (en) 2003-07-31 2010-03-23 Regulus Therapeutics Inc. Oligomeric compounds and compositions for use in modulation of small non-coding RNAs
US7846738B2 (en) 2003-08-15 2010-12-07 President And Fellows Of Harvard College Study of polymer molecules and conformations with a nanopore
US20050136408A1 (en) 2003-12-19 2005-06-23 May Tom-Moy Methods and systems for characterizing a polymer
CA2577079C (en) 2004-08-13 2014-05-20 President And Fellows Of Harvard College An ultra high-throughput opti-nanopore dna readout platform
KR101159072B1 (ko) 2005-01-20 2012-06-25 삼성전자주식회사 나노포어를 이용한 생분자의 분리방법
KR100707198B1 (ko) 2005-06-27 2007-04-13 삼성전자주식회사 나노포어 및 핵산에 비특이적으로 결합하는 물질을 이용한고감도 핵산 검출 방법
US20070190542A1 (en) 2005-10-03 2007-08-16 Ling Xinsheng S Hybridization assisted nanopore sequencing
KR100730350B1 (ko) 2005-10-17 2007-06-19 삼성전자주식회사 표면처리된 나노포어를 이용한 ｄｎａ 검출방법 및검출장치
US20070190543A1 (en) 2005-11-14 2007-08-16 Applera Corporation Coded Molecules for Detecting Target Analytes
WO2007117832A2 (en) 2006-03-12 2007-10-18 Applera Corporation Methods of detecting target nucleic acids
US8314220B2 (en) 2007-01-26 2012-11-20 Agilent Technologies, Inc. Methods compositions, and kits for detection of microRNA
WO2008097190A1 (en) 2007-02-05 2008-08-14 Agency For Science, Technology And Research Methods of electrically detecting a nucleic acid by means of an electrode pair
AU2008236694B2 (en) 2007-04-04 2014-01-23 The Regents Of The University Of California Compositions, devices, systems, and methods for using a nanopore
EP3543357A1 (en) 2007-05-08 2019-09-25 Trustees of Boston University Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof
GB0713143D0 (en) 2007-07-06 2007-08-15 Ucl Business Plc Nucleic acid detection method
WO2009045472A1 (en) 2007-10-02 2009-04-09 President And Fellows Of Harvard College Capture, recapture, and trapping of molecules with a nanopore
US20090181390A1 (en) 2008-01-11 2009-07-16 Signosis, Inc. A California Corporation High throughput detection of micrornas and use for disease diagnosis
US8852864B2 (en) 2008-01-17 2014-10-07 Sequenom Inc. Methods and compositions for the analysis of nucleic acids
EP2107040B1 (en) 2008-03-31 2011-10-26 Sony Deutschland Gmbh A method of fabricating a membrane having a tapered pore
JP2011527191A (ja) 2008-07-07 2011-10-27 オックスフォード ナノポア テクノロジーズ リミテッド 塩基検出細孔
US20100099198A1 (en) 2008-07-11 2010-04-22 Board Of Regents, The University Of Texas System Apparatus and system for pattern recognition sensing for biomolecules
WO2010062903A2 (en) 2008-11-26 2010-06-03 Board Of Regents, The University Of Texas System Genomic sequencing using modified protein pores and ionic liquids
US20110003703A1 (en) 2009-07-01 2011-01-06 Charles Ma Nucleic Acid Hybridization and Detection Using Enzymatic Reactions on a Microarray
WO2011014811A1 (en) 2009-07-31 2011-02-03 Ibis Biosciences, Inc. Capture primers and capture sequence linked solid supports for molecular diagnostic tests
WO2011028494A2 (en) 2009-08-24 2011-03-10 Trustees Of Boston University Label-free sensing of pna-dna complexes using nanopores
US8324914B2 (en) 2010-02-08 2012-12-04 Genia Technologies, Inc. Systems and methods for characterizing a molecule
WO2011103424A2 (en) 2010-02-19 2011-08-25 The Trustees Of The University Of Pennsylvania High-resolution analysis devices and related methods
US20120301886A1 (en) * 2010-02-26 2012-11-29 Michael Farrell Polytag probes
EP2553125A4 (en) * 2010-03-30 2013-10-02 Univ Boston TOOLS AND METHOD FOR NANOPORENCE PACKAGING-RELATED NUCLEIC ACID SEQUENCING
CA2805247C (en) 2010-07-14 2021-08-10 The Curators Of The University Of Missouri Nanopore-facilitated single molecule detection of nucleic acids
EP2652153B1 (en) 2010-12-17 2017-07-05 The Trustees of Columbia University in the City of New York Dna sequencing by synthesis using modified nucleotides and nanopore detection
WO2013121201A1 (en) 2012-02-15 2013-08-22 Oxford Nanopore Technologies Limited Aptamer method

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
YONG WANG ET AL, "Nanopore-based detection of circulating microRNAs in lung cancer patients", NATURE NANOTECHNOLOGY, (2011-01-01), vol. 6, no. 10, doi:10.1038/nnano.2011.147, ISSN 1748-3387, pages 668 - 674 *

Also Published As

Publication number Publication date
CA2906007C (en) 2022-06-21
CA2906007A1 (en) 2014-09-18
JP2016517275A (ja) 2016-06-16
US20180002742A1 (en) 2018-01-04
EP2971118A1 (en) 2016-01-20
EP2971118B1 (en) 2019-10-02
WO2014144217A1 (en) 2014-09-18
AU2014227754A1 (en) 2015-10-01
US9732379B2 (en) 2017-08-15
US20140309129A1 (en) 2014-10-16
EP2971118A4 (en) 2016-11-16

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