AU2014227754B2 - Encoded nanopore sensor for multiplex nucleic acids detection - Google Patents

Encoded nanopore sensor for multiplex nucleic acids detection Download PDF

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Publication number
AU2014227754B2
AU2014227754B2 AU2014227754A AU2014227754A AU2014227754B2 AU 2014227754 B2 AU2014227754 B2 AU 2014227754B2 AU 2014227754 A AU2014227754 A AU 2014227754A AU 2014227754 A AU2014227754 A AU 2014227754A AU 2014227754 B2 AU2014227754 B2 AU 2014227754B2
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probe
nucleic acid
target nucleic
probe molecule
nanopore
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AU2014227754A1 (en
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Li-qun GU
Xinyue ZHANG
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University of Missouri St Louis
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University of Missouri Columbia
University of Missouri St Louis
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Nanotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU2014227754A 2013-03-15 2014-03-14 Encoded nanopore sensor for multiplex nucleic acids detection Active AU2014227754B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361788589P 2013-03-15 2013-03-15
US61/788,589 2013-03-15
PCT/US2014/028528 WO2014144217A1 (en) 2013-03-15 2014-03-14 Encoded nanopore sensor for multiplex nucleic acids detection

Publications (2)

Publication Number Publication Date
AU2014227754A1 AU2014227754A1 (en) 2015-10-01
AU2014227754B2 true AU2014227754B2 (en) 2018-03-01

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AU2014227754A Active AU2014227754B2 (en) 2013-03-15 2014-03-14 Encoded nanopore sensor for multiplex nucleic acids detection

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US (2) US9732379B2 (https=)
EP (1) EP2971118B1 (https=)
JP (1) JP2016517275A (https=)
AU (1) AU2014227754B2 (https=)
CA (1) CA2906007C (https=)
WO (1) WO2014144217A1 (https=)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10732183B2 (en) * 2013-03-15 2020-08-04 The Trustees Of Columbia University In The City Of New York Method for detecting multiple predetermined compounds in a sample
HK1245348A1 (zh) * 2015-02-02 2018-08-24 奥特拉公司 来自样品背景的靶多核苷酸的纳米孔隙检测
WO2016196625A1 (en) 2015-06-02 2016-12-08 Nanopore Diagnostics, Llc Nucleic acid detection
WO2018093976A1 (en) 2016-11-16 2018-05-24 Nanopore Diagnostics, Llc Analysis of nucleic acids using probe with non-linear tag
US11034996B2 (en) 2018-03-02 2021-06-15 The Curators Of The University Of Missouri Nucleic acid sequence and capture by formation of an abasic site-derived cross-link
CN112292461B (zh) * 2018-06-21 2024-05-17 豪夫迈·罗氏有限公司 用于测序的隧穿结
JP7239965B2 (ja) * 2018-06-26 2023-03-15 国立大学法人東京農工大学 ２種以上の特定オリゴヌクレオチドの有無を判定する判定方法、並びに、前記判定方法を用いて検査対象が小細胞肺がん、胆管がんに罹患している又はそのリスクを有することを判定する方法
JP7344786B2 (ja) * 2019-12-19 2023-09-14 株式会社日立製作所 溶液中の任意のｄｎａ配列を同定する方法
CN112795628B (zh) * 2021-02-05 2024-02-23 南京邮电大学 基于固态纳米孔传感器的miRNA检测方法、检测探针及试剂盒

Family Cites Families (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL86164A0 (en) * 1987-04-28 1988-11-15 Tamir Biotechnology Ltd Improved dna probes
EP0304845A3 (en) * 1987-08-28 1991-03-06 Profile Diagnostic Sciences Inc. Method and kit for assaying gene expressions
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US20040152084A1 (en) * 2003-01-31 2004-08-05 Slattum Paul M. Compounds and processes for single-pot attachment of a label to nucleic acid
AU8586298A (en) 1997-07-25 1999-02-16 University Of Massachusetts Designed protein pores as components for biosensors
AU775179B2 (en) * 1999-03-15 2004-07-22 Applied Biosystems, Llc Probe/mobility modifier complexes for multiplex nucleic acid detection
WO2000056937A2 (en) * 1999-03-25 2000-09-28 Hyseq, Inc. Solution-based methods and materials for sequence analysis by hybridization
EP1212462B1 (en) 1999-09-07 2006-01-25 The Regents of the University of California Methods of determining the presence of double stranded nucleic acids in a sample
WO2001059453A2 (en) 2000-02-11 2001-08-16 The Texas A & M University System Biosensor compositions and methods of use
EP1478771A4 (en) 2001-06-21 2005-06-15 Harvard College METHODS OF CHARACTERIZING NUCLEIC ACID MOLECULES
EP1415156B1 (en) 2001-07-10 2009-09-02 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for detecting the activation state of multiple proteins in single cells
US20030165935A1 (en) * 2001-11-21 2003-09-04 Vann Charles S. Digital assay
US20050019784A1 (en) 2002-05-20 2005-01-27 Xing Su Method and apparatus for nucleic acid sequencing and identification
JP4638225B2 (ja) 2002-05-30 2011-02-23 ザ スクリプス リサーチ インスティテュート 銅を触媒とするアジドとアセチレンのライゲーション
US7683036B2 (en) 2003-07-31 2010-03-23 Regulus Therapeutics Inc. Oligomeric compounds and compositions for use in modulation of small non-coding RNAs
WO2005017025A2 (en) 2003-08-15 2005-02-24 The President And Fellows Of Harvard College Study of polymer molecules and conformations with a nanopore
US20050136408A1 (en) 2003-12-19 2005-06-23 May Tom-Moy Methods and systems for characterizing a polymer
US7972858B2 (en) 2004-08-13 2011-07-05 President And Fellows Of Harvard College Ultra high-throughput opti-nanopore DNA readout platform
KR101159072B1 (ko) 2005-01-20 2012-06-25 삼성전자주식회사 나노포어를 이용한 생분자의 분리방법
KR100707198B1 (ko) 2005-06-27 2007-04-13 삼성전자주식회사 나노포어 및 핵산에 비특이적으로 결합하는 물질을 이용한고감도 핵산 검출 방법
WO2007041621A2 (en) 2005-10-03 2007-04-12 Xingsheng Sean Ling Hybridization assisted nanopore sequencing
KR100730350B1 (ko) 2005-10-17 2007-06-19 삼성전자주식회사 표면처리된 나노포어를 이용한 ｄｎａ 검출방법 및검출장치
WO2007120265A2 (en) 2005-11-14 2007-10-25 Applera Corporation Coded molecules for detecting target analytes
US20100291548A1 (en) 2006-03-12 2010-11-18 Applera Corporation Methods of Detecting Target Nucleic Acids
US8314220B2 (en) 2007-01-26 2012-11-20 Agilent Technologies, Inc. Methods compositions, and kits for detection of microRNA
WO2008097190A1 (en) 2007-02-05 2008-08-14 Agency For Science, Technology And Research Methods of electrically detecting a nucleic acid by means of an electrode pair
EP2156179B1 (en) 2007-04-04 2021-08-18 The Regents of The University of California Methods for using a nanopore
WO2009020682A2 (en) 2007-05-08 2009-02-12 The Trustees Of Boston University Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof
GB0713143D0 (en) 2007-07-06 2007-08-15 Ucl Business Plc Nucleic acid detection method
US8273532B2 (en) 2007-10-02 2012-09-25 President And Fellows Of Harvard College Capture, recapture, and trapping of molecules with a nanopore
US20090181390A1 (en) 2008-01-11 2009-07-16 Signosis, Inc. A California Corporation High throughput detection of micrornas and use for disease diagnosis
US8852864B2 (en) 2008-01-17 2014-10-07 Sequenom Inc. Methods and compositions for the analysis of nucleic acids
EP2107040B1 (en) 2008-03-31 2011-10-26 Sony Deutschland Gmbh A method of fabricating a membrane having a tapered pore
EP2310534B1 (en) 2008-07-07 2018-09-05 Oxford Nanopore Technologies Limited Base-detecting pore
US20100099198A1 (en) 2008-07-11 2010-04-22 Board Of Regents, The University Of Texas System Apparatus and system for pattern recognition sensing for biomolecules
WO2010062903A2 (en) 2008-11-26 2010-06-03 Board Of Regents, The University Of Texas System Genomic sequencing using modified protein pores and ionic liquids
US20110003703A1 (en) 2009-07-01 2011-01-06 Charles Ma Nucleic Acid Hybridization and Detection Using Enzymatic Reactions on a Microarray
WO2011014811A1 (en) 2009-07-31 2011-02-03 Ibis Biosciences, Inc. Capture primers and capture sequence linked solid supports for molecular diagnostic tests
US20120276530A1 (en) 2009-08-24 2012-11-01 Trustees Of Boston University Label-free sensing of pna-dna complexes using nanopores
US8324914B2 (en) 2010-02-08 2012-12-04 Genia Technologies, Inc. Systems and methods for characterizing a molecule
WO2011103424A2 (en) 2010-02-19 2011-08-25 The Trustees Of The University Of Pennsylvania High-resolution analysis devices and related methods
EP2539355B1 (en) * 2010-02-26 2016-10-05 Ventana Medical Systems, Inc. In-situ hybridization with polytag probes
CN102918166A (zh) * 2010-03-30 2013-02-06 波士顿大学董事会 用于纳米孔解链依赖性核酸测序的工具和方法
WO2012009578A2 (en) 2010-07-14 2012-01-19 The Curators Of The University Of Missouri Nanopore-facilitated single molecule detection of nucleic acids
WO2012083249A2 (en) * 2010-12-17 2012-06-21 The Trustees Of Columbia University In The City Of New York Dna sequencing by synthesis using modified nucleotides and nanopore detection
IN2014DN06796A (https=) 2012-02-15 2015-05-22 Oxford Nanopore Tech Ltd

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
YONG WANG ET AL, "Nanopore-based detection of circulating microRNAs in lung cancer patients", NATURE NANOTECHNOLOGY, (2011-01-01), vol. 6, no. 10, doi:10.1038/nnano.2011.147, ISSN 1748-3387, pages 668 - 674 *

Also Published As

Publication number Publication date
US20140309129A1 (en) 2014-10-16
WO2014144217A1 (en) 2014-09-18
US9732379B2 (en) 2017-08-15
CA2906007A1 (en) 2014-09-18
CA2906007C (en) 2022-06-21
US20180002742A1 (en) 2018-01-04
EP2971118A4 (en) 2016-11-16
EP2971118A1 (en) 2016-01-20
EP2971118B1 (en) 2019-10-02
AU2014227754A1 (en) 2015-10-01
JP2016517275A (ja) 2016-06-16

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