CA2906007C - Encoded nanopore sensor for multiplex nucleic acids detection - Google Patents

Encoded nanopore sensor for multiplex nucleic acids detection Download PDF

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Publication number
CA2906007C
CA2906007C CA2906007A CA2906007A CA2906007C CA 2906007 C CA2906007 C CA 2906007C CA 2906007 A CA2906007 A CA 2906007A CA 2906007 A CA2906007 A CA 2906007A CA 2906007 C CA2906007 C CA 2906007C
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Prior art keywords
probe
nucleic acid
target nucleic
terminal extension
probe molecule
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CA2906007A
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English (en)
French (fr)
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CA2906007A1 (en
Inventor
Li-qun GU
Xinyue ZHANG
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University of Missouri St Louis
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University of Missouri St Louis
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Nanotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA2906007A 2013-03-15 2014-03-14 Encoded nanopore sensor for multiplex nucleic acids detection Active CA2906007C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361788589P 2013-03-15 2013-03-15
US61/788,589 2013-03-15
PCT/US2014/028528 WO2014144217A1 (en) 2013-03-15 2014-03-14 Encoded nanopore sensor for multiplex nucleic acids detection

Publications (2)

Publication Number Publication Date
CA2906007A1 CA2906007A1 (en) 2014-09-18
CA2906007C true CA2906007C (en) 2022-06-21

Family

ID=51537647

Family Applications (1)

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CA2906007A Active CA2906007C (en) 2013-03-15 2014-03-14 Encoded nanopore sensor for multiplex nucleic acids detection

Country Status (6)

Country Link
US (2) US9732379B2 (https=)
EP (1) EP2971118B1 (https=)
JP (1) JP2016517275A (https=)
AU (1) AU2014227754B2 (https=)
CA (1) CA2906007C (https=)
WO (1) WO2014144217A1 (https=)

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US11034996B2 (en) 2018-03-02 2021-06-15 The Curators Of The University Of Missouri Nucleic acid sequence and capture by formation of an abasic site-derived cross-link
CN112292461B (zh) * 2018-06-21 2024-05-17 豪夫迈·罗氏有限公司 用于测序的隧穿结
JP7239965B2 (ja) * 2018-06-26 2023-03-15 国立大学法人東京農工大学 ２種以上の特定オリゴヌクレオチドの有無を判定する判定方法、並びに、前記判定方法を用いて検査対象が小細胞肺がん、胆管がんに罹患している又はそのリスクを有することを判定する方法
JP7344786B2 (ja) * 2019-12-19 2023-09-14 株式会社日立製作所 溶液中の任意のｄｎａ配列を同定する方法
CN112795628B (zh) * 2021-02-05 2024-02-23 南京邮电大学 基于固态纳米孔传感器的miRNA检测方法、检测探针及试剂盒

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Also Published As

Publication number Publication date
US20140309129A1 (en) 2014-10-16
WO2014144217A1 (en) 2014-09-18
US9732379B2 (en) 2017-08-15
CA2906007A1 (en) 2014-09-18
AU2014227754B2 (en) 2018-03-01
US20180002742A1 (en) 2018-01-04
EP2971118A4 (en) 2016-11-16
EP2971118A1 (en) 2016-01-20
EP2971118B1 (en) 2019-10-02
AU2014227754A1 (en) 2015-10-01
JP2016517275A (ja) 2016-06-16

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