AU2013343537B2 - Bio-imaging method - Google Patents

Bio-imaging method Download PDF

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Publication number
AU2013343537B2
AU2013343537B2 AU2013343537A AU2013343537A AU2013343537B2 AU 2013343537 B2 AU2013343537 B2 AU 2013343537B2 AU 2013343537 A AU2013343537 A AU 2013343537A AU 2013343537 A AU2013343537 A AU 2013343537A AU 2013343537 B2 AU2013343537 B2 AU 2013343537B2
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AU
Australia
Prior art keywords
image
interest
objects
illumination
sample
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AU2013343537A
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English (en)
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AU2013343537A1 (en
Inventor
Lorene Allano
Dominique Decaux
Corine Fulchiron
Lorette Munoz
Frederic Pinston
Guillaume SUTRA
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Biomerieux SA
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Biomerieux SA
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Assigned to bioMérieux reassignment bioMérieux Request for Assignment Assignors: bioMérieux, COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES
Ceased legal-status Critical Current
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Classifications

    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/695—Preprocessing, e.g. image segmentation
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T5/00—Image enhancement or restoration
    • G06T5/77—Retouching; Inpainting; Scratch removal
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/0002—Inspection of images, e.g. flaw detection
    • G06T7/0012—Biomedical image inspection
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/12—Edge-based segmentation
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/70—Determining position or orientation of objects or cameras
    • G06T7/73—Determining position or orientation of objects or cameras using feature-based methods
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/90—Determination of colour characteristics
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/693—Acquisition
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10016—Video; Image sequence
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10024—Color image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10056—Microscopic image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10141—Special mode during image acquisition
    • G06T2207/10152—Varying illumination
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20048—Transform domain processing
    • G06T2207/20061—Hough transform
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20076—Probabilistic image processing
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20112—Image segmentation details
    • G06T2207/20152—Watershed segmentation
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30024—Cell structures in vitro; Tissue sections in vitro
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30242—Counting objects in image

Landscapes

  • Engineering & Computer Science (AREA)
  • Theoretical Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Multimedia (AREA)
  • Quality & Reliability (AREA)
  • Radiology & Medical Imaging (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Medical Informatics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Image Analysis (AREA)
AU2013343537A 2012-11-07 2013-11-07 Bio-imaging method Ceased AU2013343537B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12306372.9 2012-11-07
EP12306372.9A EP2731051A1 (en) 2012-11-07 2012-11-07 Bio-imaging method
PCT/EP2013/073291 WO2014072422A1 (en) 2012-11-07 2013-11-07 Bio-imaging method

Publications (2)

Publication Number Publication Date
AU2013343537A1 AU2013343537A1 (en) 2015-05-21
AU2013343537B2 true AU2013343537B2 (en) 2017-03-23

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
AU2013343537A Ceased AU2013343537B2 (en) 2012-11-07 2013-11-07 Bio-imaging method

Country Status (6)

Country Link
US (1) US9576181B2 (enExample)
EP (2) EP2731051A1 (enExample)
JP (1) JP6062059B2 (enExample)
CN (1) CN104903914B (enExample)
AU (1) AU2013343537B2 (enExample)
WO (1) WO2014072422A1 (enExample)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9522396B2 (en) 2010-12-29 2016-12-20 S.D. Sight Diagnostics Ltd. Apparatus and method for automatic detection of pathogens
WO2013098821A1 (en) 2011-12-29 2013-07-04 Parasight Ltd. Methods and systems for detecting a pathogen in a biological sample
WO2014188405A1 (en) 2013-05-23 2014-11-27 Parasight Ltd. Method and system for imaging a cell sample
JP2014235494A (ja) * 2013-05-31 2014-12-15 富士ゼロックス株式会社 画像処理装置及びプログラム
IL227276A0 (en) 2013-07-01 2014-03-06 Parasight Ltd A method and system for preparing a monolayer of cells, particularly suitable for diagnosis
EP3039477B1 (en) 2013-08-26 2021-10-20 S.D. Sight Diagnostics Ltd. Digital microscopy systems, methods and computer program products
ES2867811T3 (es) 2014-01-30 2021-10-20 Bd Kiestra Bv Sistema y método para obtener imágenes de muestras biológicas dispuestas en medios de cultivo
US10991096B2 (en) 2014-05-12 2021-04-27 Healthy.Io Ltd. Utilizing personal communications devices for medical testing
US10068329B2 (en) 2014-05-12 2018-09-04 Healthy.Io Ltd Method and system for automated visual analysis of a dipstick using standard user equipment
US11087467B2 (en) 2014-05-12 2021-08-10 Healthy.Io Ltd. Systems and methods for urinalysis using a personal communications device
US9972077B2 (en) * 2014-05-12 2018-05-15 Healthy.Io Ltd. Method and system for automated visual analysis of a dipstick using standard user equipment
NL2012922B1 (en) * 2014-05-30 2016-06-09 Ccm Beheer Bv Container for culturing organisms, method for monitoring the culturing of organisms inside said container, and monitoring system.
US10482595B2 (en) 2014-08-27 2019-11-19 S.D. Sight Diagnostics Ltd. System and method for calculating focus variation for a digital microscope
EP3259341B1 (en) * 2015-02-18 2021-01-27 Siemens Healthcare Diagnostics Inc. Image-based tube slot circle detection for a vision system
CN113667584B (zh) 2015-04-23 2024-12-31 Bd科斯特公司 菌落对比度收集
KR102643990B1 (ko) 2015-04-23 2024-03-05 비디 키에스트라 비.브이. 플레이팅된 배지 상의 스트리킹된 샘플로부터 자동화된 미생물 콜로니 카운트를 위한 방법 및 시스템
EP3859425B1 (en) 2015-09-17 2024-04-17 S.D. Sight Diagnostics Ltd. Methods and apparatus for detecting an entity in a bodily sample
JP6293186B2 (ja) * 2016-03-10 2018-03-14 株式会社Ｓｃｒｅｅｎホールディングス 撮像装置における撮像配置決定方法および撮像装置
CA3018536A1 (en) 2016-03-30 2017-10-05 S.D. Sight Diagnostics Ltd Distinguishing between blood sample components
EP4177593A1 (en) 2016-05-11 2023-05-10 S.D. Sight Diagnostics Ltd. Sample carrier for optical measurements
EP3455626B1 (en) 2016-05-11 2025-08-06 S.D. Sight Diagnostics Ltd. Performing optical measurements on a sample
JP7007367B2 (ja) * 2016-09-02 2022-01-24 ビオメリュー 微生物の存在を判定し、前記微生物を同定するための方法、システム、及びコンピュータプログラム製品
EP3538870B1 (en) 2016-11-14 2024-09-04 Siemens Healthcare Diagnostics Inc. Methods and apparatus for characterizing a specimen using pattern illumination
CN107316077B (zh) * 2017-06-21 2020-09-08 上海交通大学 一种基于图像分割及边缘检测的脂肪细胞自动计数方法
JP6705777B2 (ja) * 2017-07-10 2020-06-03 ファナック株式会社 機械学習装置、検査装置及び機械学習方法
WO2019070975A1 (en) 2017-10-05 2019-04-11 Becton Dickinson And Company ENVIRONMENT FOR DEVELOPING APPLICATIONS FOR EVALUATION PROCESSING OF BIOLOGICAL SAMPLES
CN111788471B (zh) 2017-11-14 2023-12-12 思迪赛特诊断有限公司 用于光学测量的样品载体
CN108102918B (zh) * 2018-01-03 2020-07-07 京东方科技集团股份有限公司 一种培养皿装置及细胞膜片的培养方法
CN112424612B (zh) * 2018-07-23 2025-03-14 株式会社岛津制作所 微流体器件观察装置及微流体器件观察方法
US10818015B2 (en) * 2019-01-28 2020-10-27 Florida Analytical Imaging Solutions, LLC. Automatic region of interest selection in centrosome analysis
MX2022004703A (es) 2019-10-22 2022-05-11 S D Sight Diagnostics Ltd Contabilidad de errores en mediciones opticas.
CN110992368A (zh) * 2019-11-08 2020-04-10 武汉互创联合科技有限公司 一种培养皿成像定位方法及装置
CN110724627B (zh) * 2019-11-15 2022-12-09 长春长光辰英生物科学仪器有限公司 单细胞分选仪的样品菌落定位装置与定位方法
CN111027527A (zh) * 2019-11-21 2020-04-17 四川极智朗润科技有限公司 一种基于光学频率特征的图像特征识别方法
BR112022011233A2 (pt) 2019-12-12 2022-08-30 S D Sight Diagnostics Ltd Detecção de plaquetas em uma amostra sanguínea
EP4073492A1 (en) 2019-12-12 2022-10-19 S.D. Sight Diagnostics Ltd. Analyzing an analyte disposed within a medium
CA3160702A1 (en) 2019-12-12 2021-06-17 S.D. Sight Diagnostics Ltd Artificial generation of a color blood smear image
CN111175300A (zh) * 2020-01-30 2020-05-19 上海市奉贤区中心医院 一种细菌耐药性产生实时观测系统
CN111223115B (zh) * 2020-04-22 2020-07-14 杭州涂鸦信息技术有限公司 一种图像分割方法、装置、设备及介质
JP2023539776A (ja) * 2020-09-04 2023-09-19 アイ―アブラ リミテッド 分析、試料容器、試料容器のキャップのための装置及び方法
BE1029446B1 (nl) * 2021-06-01 2023-01-09 Microtechnix Bvba Inrichting en werkwijze voor telling en identificatie van bacteriekolonies met behulp van hyperspectrale beeldvorming
CN113920198B (zh) * 2021-12-14 2022-02-15 纽劢科技(上海)有限公司 一种基于语义边缘对齐的由粗到精的多传感器融合定位方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6243486B1 (en) * 1996-08-30 2001-06-05 Marvin Weiss System for counting colonies of micro-organisms in petri dishes and other culture media
WO2003067904A2 (en) * 2002-02-06 2003-08-14 University Of North Carolina At Chapel Hill High-throughput cell identification and isolation method and apparatus
US20040101189A1 (en) * 2002-11-27 2004-05-27 3M Innovative Properties Company Biological growth plate scanner with automated image processing profile selection
US20040101954A1 (en) * 2002-11-27 2004-05-27 Graessle Josef A. Back side plate illumination for biological growth plate scanner

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0316471B1 (pt) * 2002-11-27 2017-06-20 3M Innovative Properies Company Device and method for squaring biological growing plates
US7319031B2 (en) 2002-11-27 2008-01-15 3M Innovative Properties Company Mounting platform for biological growth plate scanner
US7496225B2 (en) 2003-09-04 2009-02-24 3M Innovative Properties Company Biological growth plate scanner with automated intake
US7524061B2 (en) * 2005-10-12 2009-04-28 Siemens Corporate Research, Inc. System and method for robust optic disk detection in retinal images using vessel structure and radon transform
FR2926820B1 (fr) 2008-01-30 2014-10-17 Alain Rambach Procede de selection de microorganismes dans un echantillon biologique
AU2009234503B2 (en) * 2008-04-08 2014-01-16 National University Of Singapore Retinal image analysis systems and methods
JP5430122B2 (ja) 2008-10-30 2014-02-26 栄研化学株式会社 微生物の検出方法、装置およびプログラム
CN103329027A (zh) * 2011-02-28 2013-09-25 三洋电机株式会社 观察程序和观察装置
US20130094750A1 (en) * 2011-10-12 2013-04-18 Tolga Tasdizen Methods and systems for segmentation of cells for an automated differential counting system

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6243486B1 (en) * 1996-08-30 2001-06-05 Marvin Weiss System for counting colonies of micro-organisms in petri dishes and other culture media
WO2003067904A2 (en) * 2002-02-06 2003-08-14 University Of North Carolina At Chapel Hill High-throughput cell identification and isolation method and apparatus
US20040101189A1 (en) * 2002-11-27 2004-05-27 3M Innovative Properties Company Biological growth plate scanner with automated image processing profile selection
US20040101954A1 (en) * 2002-11-27 2004-05-27 Graessle Josef A. Back side plate illumination for biological growth plate scanner

Also Published As

Publication number Publication date
EP2917873A1 (en) 2015-09-16
US20150278575A1 (en) 2015-10-01
AU2013343537A1 (en) 2015-05-21
CN104903914B (zh) 2019-06-07
JP2015536140A (ja) 2015-12-21
JP6062059B2 (ja) 2017-01-18
WO2014072422A1 (en) 2014-05-15
CN104903914A (zh) 2015-09-09
US9576181B2 (en) 2017-02-21
EP2731051A1 (en) 2014-05-14

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Owner name: BIOMERIEUX

Free format text: FORMER OWNER(S): COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES; BIOMERIEUX

MK14 Patent ceased section 143(a) (annual fees not paid) or expired