CN104903914B - 用于定义细胞培养皿中感兴趣的对象周围的隔离区的方法 - Google Patents

用于定义细胞培养皿中感兴趣的对象周围的隔离区的方法 Download PDF

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Publication number
CN104903914B
CN104903914B CN201380069532.4A CN201380069532A CN104903914B CN 104903914 B CN104903914 B CN 104903914B CN 201380069532 A CN201380069532 A CN 201380069532A CN 104903914 B CN104903914 B CN 104903914B
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image
colony
sample
culture dish
interested
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Chinese (zh)
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CN104903914A (zh
Inventor
劳伦·埃拉诺
纪尧姆·苏特拉
罗瑞德·穆诺兹
科琳·菲尔希龙
D·德科
弗莱德瑞克·皮恩斯顿
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Biomerieux SA
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Biomerieux SA
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
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    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/0002—Inspection of images, e.g. flaw detection
    • G06T7/0012—Biomedical image inspection
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/695—Preprocessing, e.g. image segmentation
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T5/00—Image enhancement or restoration
    • G06T5/77—Retouching; Inpainting; Scratch removal
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/12—Edge-based segmentation
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/70—Determining position or orientation of objects or cameras
    • G06T7/73—Determining position or orientation of objects or cameras using feature-based methods
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/90—Determination of colour characteristics
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/693—Acquisition
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10016—Video; Image sequence
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10024—Color image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10056—Microscopic image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10141—Special mode during image acquisition
    • G06T2207/10152—Varying illumination
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20048—Transform domain processing
    • G06T2207/20061—Hough transform
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20076—Probabilistic image processing
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20112—Image segmentation details
    • G06T2207/20152—Watershed segmentation
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30024—Cell structures in vitro; Tissue sections in vitro
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30242—Counting objects in image

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  • Engineering & Computer Science (AREA)
  • Theoretical Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Multimedia (AREA)
  • Quality & Reliability (AREA)
  • Radiology & Medical Imaging (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Medical Informatics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Image Analysis (AREA)
CN201380069532.4A 2012-11-07 2013-11-07 用于定义细胞培养皿中感兴趣的对象周围的隔离区的方法 Expired - Fee Related CN104903914B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12306372.9 2012-11-07
EP12306372.9A EP2731051A1 (en) 2012-11-07 2012-11-07 Bio-imaging method
PCT/EP2013/073291 WO2014072422A1 (en) 2012-11-07 2013-11-07 Bio-imaging method

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CN104903914A CN104903914A (zh) 2015-09-09
CN104903914B true CN104903914B (zh) 2019-06-07

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US (1) US9576181B2 (enExample)
EP (2) EP2731051A1 (enExample)
JP (1) JP6062059B2 (enExample)
CN (1) CN104903914B (enExample)
AU (1) AU2013343537B2 (enExample)
WO (1) WO2014072422A1 (enExample)

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US12140743B2 (en) * 2018-07-23 2024-11-12 Shimadzu Corporation Microfluidic device observation device and microfluidic device observation method
US10818015B2 (en) * 2019-01-28 2020-10-27 Florida Analytical Imaging Solutions, LLC. Automatic region of interest selection in centrosome analysis
CN110992368A (zh) * 2019-11-08 2020-04-10 武汉互创联合科技有限公司 一种培养皿成像定位方法及装置
CN110724627B (zh) * 2019-11-15 2022-12-09 长春长光辰英生物科学仪器有限公司 单细胞分选仪的样品菌落定位装置与定位方法
CN111027527A (zh) * 2019-11-21 2020-04-17 四川极智朗润科技有限公司 一种基于光学频率特征的图像特征识别方法
JP7677972B2 (ja) 2019-12-12 2025-05-15 エス．ディー．サイト ダイアグノスティックス リミテッド カラー血液スミア画像の人工的な生成
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Also Published As

Publication number Publication date
AU2013343537A1 (en) 2015-05-21
JP6062059B2 (ja) 2017-01-18
EP2917873A1 (en) 2015-09-16
US20150278575A1 (en) 2015-10-01
JP2015536140A (ja) 2015-12-21
WO2014072422A1 (en) 2014-05-15
AU2013343537B2 (en) 2017-03-23
CN104903914A (zh) 2015-09-09
US9576181B2 (en) 2017-02-21
EP2731051A1 (en) 2014-05-14

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