AU2013240127B2 - Immunofluorescence and fluorescent-based nucleic acid analysis on a single sample - Google Patents

Immunofluorescence and fluorescent-based nucleic acid analysis on a single sample Download PDF

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Publication number
AU2013240127B2
AU2013240127B2 AU2013240127A AU2013240127A AU2013240127B2 AU 2013240127 B2 AU2013240127 B2 AU 2013240127B2 AU 2013240127 A AU2013240127 A AU 2013240127A AU 2013240127 A AU2013240127 A AU 2013240127A AU 2013240127 B2 AU2013240127 B2 AU 2013240127B2
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image
sample
nucleic acid
biological sample
binder
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AU2013240127A
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AU2013240127A1 (en
Inventor
Alex D. Corwin
Michael J. Gerdes
Fiona Ginty
Thomas Ha
David Lavan Henderson
Denise A HOLLMAN-HEWGLEY
Natalie R. Jun
Kevin B. Kenny
Ainura KYSHTOOBAYEVE
Adriana I. Larriera Moreno
Ying Li
Xiaofeng Liu
Antti E. SEPPO
Stephen E. Zingelewicz
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Leica Microsystems CMS GmbH
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Leica Microsystems CMS GmbH
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57407—Specifically defined cancers
    • G01N33/57415—Specifically defined cancers of breast
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57407—Specifically defined cancers
    • G01N33/57423—Specifically defined cancers of lung
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/71—Assays involving receptors, cell surface antigens or cell surface determinants for growth factors; for growth regulators

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU2013240127A 2012-03-30 2013-03-21 Immunofluorescence and fluorescent-based nucleic acid analysis on a single sample Active AU2013240127B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261618132P 2012-03-30 2012-03-30
US61/618,132 2012-03-30
PCT/US2013/033249 WO2013148448A1 (en) 2012-03-30 2013-03-21 Immunoflu0rescence and fluorescent-based nucleic acid analysis on a single sample

Publications (2)

Publication Number Publication Date
AU2013240127A1 AU2013240127A1 (en) 2014-10-02
AU2013240127B2 true AU2013240127B2 (en) 2018-07-12

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US (2) US20150065371A1 (cg-RX-API-DMAC7.html)
EP (1) EP2831591B1 (cg-RX-API-DMAC7.html)
JP (1) JP6225163B2 (cg-RX-API-DMAC7.html)
KR (1) KR20140142715A (cg-RX-API-DMAC7.html)
CN (1) CN104520713A (cg-RX-API-DMAC7.html)
AU (1) AU2013240127B2 (cg-RX-API-DMAC7.html)
CA (1) CA2867508C (cg-RX-API-DMAC7.html)
IN (1) IN2014DN07531A (cg-RX-API-DMAC7.html)
NZ (1) NZ630091A (cg-RX-API-DMAC7.html)
RU (1) RU2014137129A (cg-RX-API-DMAC7.html)
SG (1) SG11201406105UA (cg-RX-API-DMAC7.html)
WO (1) WO2013148448A1 (cg-RX-API-DMAC7.html)

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WO2015124737A1 (en) 2014-02-24 2015-08-27 Ventana Medical Systems, Inc. Methods, kits, and systems for scoring the immune response to cancer by simultaneous detection of cd3, cd8, cd20 and foxp3
CN104980558B (zh) * 2014-04-01 2019-01-01 中国移动通信集团公司 一种信息处理的方法、装置、手机宠物客户端及移动终端
US20150286887A1 (en) * 2014-04-07 2015-10-08 Massachusetts Institute Of Technology Use of Microparticle Additives to Simultaneously Enable Artifact-Free Image Registration, Auto-Focusing, and Chromatic Aberration Correction in Microscopy
WO2016026038A1 (en) * 2014-08-18 2016-02-25 Viewsiq Inc. System and method for embedded images in large field-of-view microscopic scans
EP3227684B1 (en) 2014-12-03 2019-10-02 Isoplexis Corporation Analysis and screening of cell secretion profiles
US11353448B2 (en) 2015-02-13 2022-06-07 California Institute Of Technology Methods and compositions for quantifying metabolites and proteins from single cells
WO2017012555A1 (zh) * 2015-07-20 2017-01-26 徐敏 光子结构和化学计量学病理系统
JP6702339B2 (ja) * 2015-12-24 2020-06-03 コニカミノルタ株式会社 画像処理装置及びプログラム
CN107419001A (zh) * 2016-05-23 2017-12-01 益善生物技术股份有限公司 Brca1、pten基因异常检测试剂盒及检测方法
JP6231709B1 (ja) * 2016-05-31 2017-11-15 シスメックス株式会社 蛍光画像分析装置および分析方法
JP6755975B2 (ja) * 2016-06-10 2020-09-16 エフ・ホフマン−ラ・ロシュ・アクチェンゲゼルシャフト 明視野像シミュレーションのためのシステム
CN106290878A (zh) * 2016-09-10 2017-01-04 天津大学 基于量子点的胰腺癌肿瘤标志物ca242免疫层析试纸条的制备方法
CN109964126B (zh) 2016-09-12 2022-12-27 伊索普莱克西斯公司 用于细胞治疗法和其他免疫治疗法的多重分析的系统和方法
IL265478B2 (en) * 2016-09-26 2024-10-01 Becton Dickinson Co Measurement of protein expression using reagents with barcoded oligonucleotide sequences
EP3538891B1 (en) 2016-11-11 2022-01-05 Isoplexis Corporation Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells
US11525783B2 (en) 2016-11-22 2022-12-13 IsoPlexis Corporation Systems, devices and methods for cell capture and methods of manufacture thereof
JP7116175B2 (ja) * 2018-08-22 2022-08-09 オリンパス株式会社 画像処理装置、撮像システム、画像処理装置の作動方法、及び画像処理装置の作動プログラム
US10921223B2 (en) * 2018-10-12 2021-02-16 Shenzhen Prs Limited Process record slide for staining and method of using the same
CN110498858B (zh) * 2019-07-26 2024-01-23 深圳市达科为生物工程有限公司 一种动态检测单细胞外泌蛋白分泌情况的方法
WO2021127019A1 (en) * 2019-12-17 2021-06-24 Applied Materials, Inc. System and method for acquisition and processing of multiplexed fluorescence in-situ hybridization images
CN115702349A (zh) * 2020-05-07 2023-02-14 领先细胞医疗诊断有限公司 用于同时检测靶核酸和蛋白质的方法及其试剂盒
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Also Published As

Publication number Publication date
US20250102507A1 (en) 2025-03-27
CA2867508C (en) 2021-05-18
NZ630091A (en) 2016-03-31
KR20140142715A (ko) 2014-12-12
CA2867508A1 (en) 2013-10-03
JP2015514212A (ja) 2015-05-18
WO2013148448A1 (en) 2013-10-03
EP2831591A1 (en) 2015-02-04
EP2831591B1 (en) 2019-05-08
AU2013240127A1 (en) 2014-10-02
US20150065371A1 (en) 2015-03-05
IN2014DN07531A (cg-RX-API-DMAC7.html) 2015-04-24
CN104520713A (zh) 2015-04-15
RU2014137129A (ru) 2016-05-27
JP6225163B2 (ja) 2017-11-01
SG11201406105UA (en) 2014-10-30

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