AU2012294724B2 - Compositions and methods relating to nucleic acid nano-and micro-technology - Google Patents
Compositions and methods relating to nucleic acid nano-and micro-technology Download PDFInfo
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- AU2012294724B2 AU2012294724B2 AU2012294724A AU2012294724A AU2012294724B2 AU 2012294724 B2 AU2012294724 B2 AU 2012294724B2 AU 2012294724 A AU2012294724 A AU 2012294724A AU 2012294724 A AU2012294724 A AU 2012294724A AU 2012294724 B2 AU2012294724 B2 AU 2012294724B2
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Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
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| PCT/US2012/049306 WO2013022694A1 (en) | 2011-08-05 | 2012-08-02 | Compositions and methods relating to nucleic acid nano-and micro-technology |
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| EP2633071B1 (en) | 2010-10-27 | 2016-10-12 | President and Fellows of Harvard College | Compositions of toehold primer duplexes and methods of use |
| US9796748B2 (en) | 2011-05-02 | 2017-10-24 | President And Fellows Of Harvard College | Spatial sequestration of dynamic nucleic acid circuits |
| JP2014525921A (ja) | 2011-08-05 | 2014-10-02 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 核酸ナノテクノロジー及びマイクロテクノロジーに関する組成物及び方法 |
| US9073962B2 (en) | 2012-07-12 | 2015-07-07 | Massachusetts Institute Of Technology | Methods of serial assembly of DNA bricks into larger structures |
| WO2014018675A1 (en) | 2012-07-24 | 2014-01-30 | President And Fellows Of Harvard College | Self-assembly of nucleic acid nanostructures |
| WO2014074597A1 (en) | 2012-11-06 | 2014-05-15 | President And Fellows Of Harvard College | Compositions and methods relating to complex nucleic acid nanostructures |
| EP3594363A1 (en) | 2014-04-23 | 2020-01-15 | Children's Medical Center Corporation | High-throughput structure determination using nucleic acid calipers |
| JP2017523594A (ja) | 2014-05-22 | 2017-08-17 | プレジデント アンド フェローズ オブ ハーバード カレッジ | スケーラブルな核酸をベースにしたナノファブリケーション |
| US11639522B2 (en) | 2015-01-30 | 2023-05-02 | President And Fellows Of Harvard College | Microscope-free imaging |
| WO2016144755A1 (en) | 2015-03-07 | 2016-09-15 | President And Fellows Of Harvard College | Single-stranded dna nanostructures |
| CN105602949A (zh) * | 2016-01-29 | 2016-05-25 | 同济大学 | 链间交换由支架dna达成的核酸结构及其合成方法 |
| US11079388B2 (en) | 2016-04-26 | 2021-08-03 | Ultivue, Inc. | Super-resolution immunofluorescence with diffraction-limited preview |
| CN109563539A (zh) | 2016-06-15 | 2019-04-02 | 慕尼黑路德维希马克西米利安斯大学 | 使用dna纳米技术的单分子检测或定量 |
| CN106517087B (zh) * | 2017-01-06 | 2018-09-28 | 中国科学院苏州纳米技术与纳米仿生研究所 | 纳米颗粒螺旋和纳米棒组合结构及其构建方法 |
| WO2018213372A1 (en) | 2017-05-16 | 2018-11-22 | President And Fellows Of Harvard College | Nucleic acid-lined nanodiscs |
| WO2018218646A1 (zh) * | 2017-06-02 | 2018-12-06 | 清华大学 | Dna折纸单元分步组装法 |
| EP3696275B1 (en) * | 2017-10-11 | 2025-12-24 | MGI Tech Co., Ltd. | Method for improving loading and stability of nucleic acid on solid support |
| CN112189055B (zh) | 2018-03-22 | 2024-07-19 | 哈佛学院院长及董事 | 用于分子认证的方法和组合物 |
| EP3775196A4 (en) | 2018-04-04 | 2021-12-22 | Nautilus Biotechnology, Inc. | METHOD FOR GENERATING NANOARRAYS AND MICROARRAYS |
| MA52750A (fr) | 2018-06-05 | 2021-04-14 | Univ Muenchen Tech | Nouveau procédé de stabilisation de nanostructures d'acide nucléique |
| CA3135206A1 (en) | 2019-04-29 | 2020-11-05 | Pierre Indermuhle | Methods and systems for integrated on-chip single-molecule detection |
| US10777381B1 (en) * | 2019-08-08 | 2020-09-15 | Ut-Battelle, Llc | Beam controlled nano-robotic device |
| AU2021378290B2 (en) | 2020-11-11 | 2025-10-30 | Nautilus Subsidiary, Inc. | Affinity reagents having enhanced binding and detection characteristics |
| IL305336B2 (en) | 2021-03-11 | 2025-09-01 | Nautilus Subsidiary Inc | Systems and methods for preserving biomolecules |
| CA3227872A1 (en) | 2021-09-09 | 2023-03-16 | James Henry JOLY | Characterization and localization of protein modifications |
| AU2022402132A1 (en) | 2021-11-30 | 2024-05-30 | Nautilus Subsidiary, Inc. | Particle-based isolation of proteins and other analytes |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6255469B1 (en) * | 1998-05-06 | 2001-07-03 | New York University | Periodic two and three dimensional nucleic acid structures |
| US20080221315A1 (en) * | 2006-12-07 | 2008-09-11 | New York University | Nucleic acid-based translation system and method for decoding nucleic acid encrypted message |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6444650B1 (en) | 1996-10-01 | 2002-09-03 | Geron Corporation | Antisense compositions for detecting and inhibiting telomerase reverse transcriptase |
| US6031098A (en) | 1997-08-11 | 2000-02-29 | California Institute Of Technology | Detection and treatment of duplex polynucleotide damage |
| AU782880B2 (en) | 1999-11-13 | 2005-09-08 | Merck Patent Gmbh | High order nucleic acid based structures |
| JP4340779B2 (ja) | 2000-10-05 | 2009-10-07 | 独立行政法人理化学研究所 | 可変突出部位を含むオリゴヌクレオチドリンカー及び前記リンカーを用いたポリヌクレオチドライブラリーの調製方法 |
| US7612184B2 (en) * | 2002-02-22 | 2009-11-03 | New York University | Polynucleic acid nanomechanical device controlled by hybridization topology |
| JP4061401B2 (ja) * | 2002-03-07 | 2008-03-19 | 国立大学法人九州大学 | Ｄｎａの自己組織化によるｄｎａナノケージ及びその製造方法、並びにそれを用いたｄｎａナノチューブ、分子キャリアー |
| WO2005024018A1 (ja) | 2003-09-08 | 2005-03-17 | Zoegene Corporation | 核酸構築物およびその製造方法 |
| CA2570108A1 (en) * | 2004-06-10 | 2006-08-17 | New York University | Polygonal nanostructures of polynucleic acid multi-crossover molecules and assembly of lattices based on double crossover cohesion |
| WO2006017432A2 (en) * | 2004-08-02 | 2006-02-16 | New York University | A polynucleotide nanomechanical device that acts as an artificial ribosome and translates dna signals into polymer assembly instructions |
| US7842793B2 (en) | 2005-06-14 | 2010-11-30 | The California Institute Of Technology | Methods of making nucleic acid nanostructures |
| US7745594B2 (en) | 2005-07-21 | 2010-06-29 | California Institute Of Technology | Nucleic acid-based logic circuits |
| WO2007012807A2 (en) | 2005-07-27 | 2007-02-01 | University Of Leeds | Dna structures |
| WO2007103558A2 (en) | 2006-03-09 | 2007-09-13 | The Regents Of The University Of California | Hybrid energy transfer for nucleic acid detection |
| CN101541961A (zh) * | 2006-10-04 | 2009-09-23 | 布鲁克哈文科学协会 | Dna指导的纳米颗粒组装体 |
| WO2008130585A2 (en) | 2007-04-17 | 2008-10-30 | Dana-Farber Cancer Institute | Wireframe nanostructures |
| WO2009093558A1 (ja) * | 2008-01-22 | 2009-07-30 | Tokyo Institute Of Technology | 核酸構造体及びその核酸構造体を用いた核酸構造物、並びにその核酸構造物の作製方法 |
| US20100291485A1 (en) | 2008-09-14 | 2010-11-18 | Troy Lapsys | Nanoscale molecule synthesis |
| WO2010148085A1 (en) * | 2009-06-16 | 2010-12-23 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Rna nanoparticles and methods of use |
| WO2011106057A2 (en) | 2009-12-04 | 2011-09-01 | Trustees Of Boston University | Nanostructure biosensors and systems and methods of use thereof |
| US8877438B2 (en) | 2010-07-20 | 2014-11-04 | California Institute Of Technology | Self-assembled polynucleotide structure |
| WO2012058638A2 (en) | 2010-10-29 | 2012-05-03 | President And Fellows Of Harvard College | Nucleic acid nanostructure barcode probes |
| US20130316358A1 (en) | 2011-01-31 | 2013-11-28 | Yeda Research And Development Co. Ltd. | Methods of diagnosing disease using overlap extension pcr |
| GB201111237D0 (en) | 2011-06-30 | 2011-08-17 | Isis Innovation | Nanochip |
| JP2014525921A (ja) | 2011-08-05 | 2014-10-02 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 核酸ナノテクノロジー及びマイクロテクノロジーに関する組成物及び方法 |
| WO2014018675A1 (en) | 2012-07-24 | 2014-01-30 | President And Fellows Of Harvard College | Self-assembly of nucleic acid nanostructures |
| WO2014074597A1 (en) | 2012-11-06 | 2014-05-15 | President And Fellows Of Harvard College | Compositions and methods relating to complex nucleic acid nanostructures |
| CN106459132A (zh) | 2014-03-08 | 2017-02-22 | 哈佛学院院长及董事 | 由自组装的含顶点的固定角度核酸结构形成的核酸多面体 |
-
2012
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- 2012-08-02 SG SG10201606482TA patent/SG10201606482TA/en unknown
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6255469B1 (en) * | 1998-05-06 | 2001-07-03 | New York University | Periodic two and three dimensional nucleic acid structures |
| US20080221315A1 (en) * | 2006-12-07 | 2008-09-11 | New York University | Nucleic acid-based translation system and method for decoding nucleic acid encrypted message |
Non-Patent Citations (1)
| Title |
|---|
| YIN, P. ET. AL. Science, 2008, 321, 824-826 * |
Also Published As
| Publication number | Publication date |
|---|---|
| EP2739638A1 (en) | 2014-06-11 |
| JP2014525921A (ja) | 2014-10-02 |
| WO2013022694A1 (en) | 2013-02-14 |
| CN107698640A (zh) | 2018-02-16 |
| AU2012294724A1 (en) | 2014-03-06 |
| US9796749B2 (en) | 2017-10-24 |
| KR20140054179A (ko) | 2014-05-08 |
| SG10201606482TA (en) | 2016-10-28 |
| CN103889998A (zh) | 2014-06-25 |
| EP2739638B1 (en) | 2017-10-04 |
| CA2849072A1 (en) | 2013-02-14 |
| EP2739638A4 (en) | 2015-04-08 |
| US20140213778A1 (en) | 2014-07-31 |
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