AU2012256014B2 - Use of Zscan4 and Zscan4-dependent genes for direct reprogramming of somatic cells - Google Patents

Use of Zscan4 and Zscan4-dependent genes for direct reprogramming of somatic cells Download PDF

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AU2012256014B2
AU2012256014B2 AU2012256014A AU2012256014A AU2012256014B2 AU 2012256014 B2 AU2012256014 B2 AU 2012256014B2 AU 2012256014 A AU2012256014 A AU 2012256014A AU 2012256014 A AU2012256014 A AU 2012256014A AU 2012256014 B2 AU2012256014 B2 AU 2012256014B2
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zscan4
cell
cells
protein
reprogramming
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AU2012256014A1 (en
AU2012256014A8 (en
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Tetsuya Hirata
Minoru S.H. Ko
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Engins LLC
United States Department of Health and Human Services
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Engins LLC
United States Department of Health and Human Services
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Assigned to THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY, DEPT. OF HEALTH AND HUMAN SERVICES, ELIXIRGEN, LLC reassignment THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY, DEPT. OF HEALTH AND HUMAN SERVICES Amend patent request/document other than specification (104) Assignors: THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY, DEPT. OF HEALTH AND HUMAN SERVICES
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    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]

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AU2012256014A 2011-05-13 2012-05-11 Use of Zscan4 and Zscan4-dependent genes for direct reprogramming of somatic cells Active AU2012256014B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201161486004P 2011-05-13 2011-05-13
US61/486,004 2011-05-13
US201161529055P 2011-08-30 2011-08-30
US61/529,055 2011-08-30
PCT/US2012/037643 WO2012158561A1 (en) 2011-05-13 2012-05-11 Use of zscan4 and zscan4-dependent genes for direct reprogramming of somatic cells

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AU2012256014A1 AU2012256014A1 (en) 2013-12-19
AU2012256014A8 AU2012256014A8 (en) 2014-01-09
AU2012256014B2 true AU2012256014B2 (en) 2016-10-27

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US (1) US9932560B2 (enExample)
EP (1) EP2707479B1 (enExample)
JP (2) JP6189829B2 (enExample)
AU (1) AU2012256014B2 (enExample)
CA (1) CA2835825C (enExample)
WO (1) WO2012158561A1 (enExample)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012158561A1 (en) 2011-05-13 2012-11-22 The United States Of America As Represented By The Secretary, Dept. Of Health And Human Services Use of zscan4 and zscan4-dependent genes for direct reprogramming of somatic cells
EP2968392B1 (en) * 2013-03-15 2020-08-05 Elixirgen Therapeutics, Inc. Methods of using zscan4 for rejuvenating human cells
US20170327796A1 (en) * 2014-12-18 2017-11-16 University Of Utah Research Foundation Induced pluripotent stem cell and method for producing the same
CN105219729B (zh) * 2015-09-28 2018-09-25 首都医科大学宣武医院 一种利用非整合质粒载体诱导神经干细胞的方法及其用途
CN106800590B (zh) * 2015-11-25 2021-04-09 杭州智鹤丹谷生物医药有限公司 一种结合多种淀粉样蛋白单体和聚集体的多肽及其应用
CN110268072B (zh) * 2016-12-15 2023-11-07 Illumina公司 确定旁系同源基因的方法和系统
CN107475378B (zh) * 2017-08-03 2021-03-30 复旦大学 用于检测女性原发不孕的patl2基因及检测该基因突变的试剂盒
WO2019060450A1 (en) * 2017-09-19 2019-03-28 The Broad Institute, Inc. METHODS AND SYSTEMS FOR RECONSTRUCTION OF DEVELOPMENT LANDSCAPES BY OPTIMAL TRANSPORT ANALYSIS
CN107988147B (zh) * 2017-12-19 2021-11-09 江汉大学 基于器官芯片与诱导多能干细胞的定向分化用于3d拟表皮构建的方法
US12540173B2 (en) * 2019-05-22 2026-02-03 University Of Maryland, Baltimore Methods of treating cancer by targeting ZSCAN4 activity in cancer stem cells
CN110616232B (zh) * 2019-09-23 2023-04-11 中国人民解放军陆军军医大学 Sox30敲除和可恢复型小鼠动物模型构建方法及应用
BR112022009898A2 (pt) * 2019-11-22 2022-09-06 Alcamena Stem Cell Therapeutics Llc Composições e métodos para desrepressão de genes alvo do fator de transcrição silenciador de re1

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011028880A2 (en) * 2009-09-04 2011-03-10 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Methods for enhancing genome stability and telomere elongation in embryonic stem cells

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4980289A (en) 1987-04-27 1990-12-25 Wisconsin Alumni Research Foundation Promoter deficient retroviral vector
US5888762A (en) 1990-06-05 1999-03-30 Centre National De La Recherche Scientifique (Cnrs) Neurotropic growth factors comprising a homeobox peptide
KR100201352B1 (ko) 1995-03-16 1999-06-15 성재갑 단일주사 백신 제형
US7198784B2 (en) 1996-10-17 2007-04-03 Oxford Biomedica (Uk) Limited Retroviral vectors
FR2762615B1 (fr) 1997-04-28 1999-07-16 Inst Nat Sante Rech Med Nouveau site interne d'entree des ribosomes et vecteur le contenant
EP1188835A4 (en) 1999-06-22 2004-07-07 Dnavec Research Inc A VECTOR FOR THE EXPRESSION OF TWO FOREIGN GENES
US20110171185A1 (en) * 1999-06-30 2011-07-14 Klimanskaya Irina V Genetically intact induced pluripotent cells or transdifferentiated cells and methods for the production thereof
DE19933492B4 (de) 1999-07-16 2008-01-10 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Konjugat zur Vermittlung eines zell-, kompartiment- oder membranspezifischen Transports von Wirksubstanzen, Verfahren zu dessen Herstellung und dessen Verwendung
JP2003514564A (ja) 1999-11-24 2003-04-22 エムシーエス マイクロ キャリア システムズ ゲーエムベーハー 核局在化シグナルまたはタンパク質導入領域の多量体を含むポリペプチド、および分子を細胞内へ移入するためのその使用法
US7211247B2 (en) 2001-04-09 2007-05-01 University Of Southern California Lentivirus vectors for gene transfer to alveolar epithelial cells
US7081489B2 (en) 2001-08-09 2006-07-25 Florida State University Research Foundation Polymeric encapsulation of nanoparticles
US20040014209A1 (en) 2002-01-23 2004-01-22 Lassar Andrew B. Compositions and methods for modulating cell differentiation
US7354763B2 (en) 2002-04-16 2008-04-08 Technion Research & Development Foundation Ltd. Generating vascular smooth muscle cells in vitro from ES cells
US20050287648A1 (en) 2002-08-05 2005-12-29 University Of Rochester Protein Transducing Domain/Deaminase Chimeric Proteins, Related Compounds, and Uses Thereof
KR100591936B1 (ko) 2002-11-12 2006-06-22 포휴먼텍(주) 세포내 ｄｎａ／ｒｎａ 전달 방법, 및 이것의 기초 및임상학적 응용
EP1583473A2 (en) 2003-01-16 2005-10-12 St. Johns University New York Nanoparticle based stabilization of ir fluorescent dyes
US7166692B2 (en) 2003-03-04 2007-01-23 Canbrex Bio Science Walkersville, Inc. Intracellular delivery of small molecules, proteins, and nucleic acids
US7682828B2 (en) 2003-11-26 2010-03-23 Whitehead Institute For Biomedical Research Methods for reprogramming somatic cells
CA2937099A1 (en) 2004-01-23 2005-08-04 Astellas Institute For Regenerative Medicine Improved modalities for the treatment of degenerative diseases of the retina
WO2006052285A2 (en) 2004-05-13 2006-05-18 The Trustees Of Columbia University In The City Of New York Polymeric nanoparticles and nanogels for extraction and release of compounds
US20060024331A1 (en) 2004-08-02 2006-02-02 Ester Fernandez-Salas Toxin compounds with enhanced membrane translocation characteristics
AU2006210955A1 (en) 2005-01-31 2006-08-10 Es Cell International Pte Ltd. Directed differentiation of embryonic stem cells and uses thereof
US7342048B2 (en) 2005-04-28 2008-03-11 Nipro Corporation Bioabsorbable pharmaceutical formulation
EP1906985A4 (en) 2005-05-10 2012-07-11 Naomi Balaban COMPOSITIONS FOR ADMINISTERING RNAIII-INHIBITING PEPTIDES
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
EP2206724A1 (en) 2005-12-13 2010-07-14 Kyoto University Nuclear reprogramming factor
US20090227032A1 (en) 2005-12-13 2009-09-10 Kyoto University Nuclear reprogramming factor and induced pluripotent stem cells
WO2008118957A2 (en) 2007-03-26 2008-10-02 The Government Of U.S.A As Represented By The Secretary Of The Department Of Health & Human Services Methods for modulating embryonic stem cell differentiation
JP5346925B2 (ja) 2008-05-02 2013-11-20 国立大学法人京都大学 核初期化方法
CA2830600C (en) 2011-03-23 2021-07-27 Tomokazu AMANO Compositions and methods for enhancing the pluripotency of stem cells
WO2012158561A1 (en) 2011-05-13 2012-11-22 The United States Of America As Represented By The Secretary, Dept. Of Health And Human Services Use of zscan4 and zscan4-dependent genes for direct reprogramming of somatic cells

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011028880A2 (en) * 2009-09-04 2011-03-10 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Methods for enhancing genome stability and telomere elongation in embryonic stem cells

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JP2017212985A (ja) 2017-12-07
CA2835825C (en) 2021-03-23
JP2014513983A (ja) 2014-06-19
US20140287511A1 (en) 2014-09-25
EP2707479B1 (en) 2018-01-10
WO2012158561A1 (en) 2012-11-22
US9932560B2 (en) 2018-04-03
EP2707479A4 (en) 2014-11-26
JP6189829B2 (ja) 2017-08-30
AU2012256014A1 (en) 2013-12-19
CA2835825A1 (en) 2012-11-22
AU2012256014A8 (en) 2014-01-09
EP2707479A1 (en) 2014-03-19

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FGA Letters patent sealed or granted (standard patent)
HB Alteration of name in register

Owner name: ENGINS, LLC

Free format text: FORMER NAME(S): ELIXIRGEN, LLC; THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY, DEPT. OF HEALTH AND HUMAN SERVICES

Owner name: THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY, DEPT. OF HEALTH AND HUMAN SERVICES

Free format text: FORMER NAME(S): ELIXIRGEN, LLC; THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY, DEPT. OF HEALTH AND HUMAN SERVICES