AU2009334514A1 - Uses of thaxtomin and thaxtomin compositions as herbicides - Google Patents

Abstract

There is a need for a selective, low-risk herbicide that can be used to control weeds in cereal cultures and turf. The present invention discloses that a bacterial secondary metabolite, thaxtomin and optionally another herbicide is an effective herbicide on broadleaved, sedge and grass weeds. Thaxtomin A and structurally similar compounds can be used as natural herbicides to control the germination and growth of weeds in cereal, turf grass, Timothy grass and pasture grass cultures with no phytotoxicity to these crops. As a natural, non-toxic compound, thaxtomin can be used as a safe alternative for weed control in both conventional and organic farming and gardening systems.

Description

WO 2010/078452 PCT/US2009/069856 USES OF THAXTOMIN AND THAXTOMIN COMPOSITIONS AS HERBICIDES FIELD OF THE INVENTION This invention relates to compositions and methods for controlling the germination and 5 growth of broadleaf, sedge and grass weeds using compounds comprising thaxtomin, a cyclic dipeptide produced by Streptomyces sp., as an active ingredient. BACKGROUND OF THE INVENTION Natural products are substances produced by microbes, plants, and other organisms. 10 Microbial natural products offer an abundant source of chemical diversity, and there is a long history of utilizing natural products for pharmaceutical purposes. However, secondary metabolites produced by microbes can also be successfully used for weed and pest control in agricultural applications. Thaxtomins (4-nitroindol-3-yl-containing 2,5-dioxopiperazines) are a family of dipeptide phytotoxins produced by plant-pathogenic Streptomyces sp. (S. scabies, S. acidiscabies) that cause 15 scab diseases in potato (Solanum tuberosum) (King, Lawrence et al. 1992). Toxin production occurs in diseased tissue and can also be elicited in vitro in an optimal growth medium containing oat bran (Loria, Bukhalid et al. 1995; Beaus6jour, Goyer et al. 1999). King and her coworkers (King, Lawrence et al. 2001) demonstrated that all plant pathogenic species in the Streptomyces family produce one or more thaxtomins with herbicidal activity. Hiltunen et al. (Hiltunen, Laakso et al. 20 2006) purified four thaxtomin analogs (thaxtomin A, thaxtomin A ortho isomer, thaxtomin B and thaxtomin D) from cultures of S. scabies and S. turbidiscabies and showed that all four compounds induced similar symptoms of reduced shoot and root growth, root swelling, (at 10-200 ppb) and necrosis (at 200-1000 ppb) on micropropagated in vitro cultures of potato. In addition, thaxtomins applied in combinations, showed additive effects, but no synergism (Hiltunen, Laakso et al. 2006). 25 According to Duke et al. (Duke, Baerson et al. 2003), both thaxtomin A (Figure 1) and thaxtomin D have marked activity as pre and post emergent, non-systemic herbicides, and concentrations of less than 1 uM of thaxtomin A causes cell swelling, necrosis and growth inhibition in mono and dicotyledonous seedlings (Healy, Wach et al. 2000). Thaxtomin has been evaluated as an herbicide by Dow Agro Sciences, Inc., and while active, it lacked systemic action (King, Lawrence et al. 30 2001). The presence of the nitro group in the indole ring required for an L,L- configuration of the diketopiperazine appears to be the minimal requirement for phytotoxicity. The position of the nitro 1 WO 2010/078452 PCT/US2009/069856 group in the indole ring is very site specific, and the phenyl portion of the phenylalanine plays a necessary role in structural requirements of phytotoxicity (King, Lawrence et al. 1989; King, Lawrence et al. 1992; King, Lawrence et al. 2003). The herbicidal mode of action is based on disruption of cell wall synthesis (Fry and Loria 2002), with inhibition of cellulose biosynthesis being 5 the main target (King et al., 2001; Duval et al., 2005; Johnson et al. 2007). Recently, Kang et al. (Kang, Semones et al. 2008) have described the use of thaxtomin and thaxtomin compositions as algaecides to control algae in water environments. SUMMARY OF THE INVENTION 10 The present invention discloses the use of thaxtomin as a pre or post-emergence herbicide against most common weeds in the cereal, pasture grass, Timothy grasses and turf grass growth systems. A "growth system" may be any ecosystem for growing cereal, pasture grass, Timothy grass and turf grass. For example, a "cereal growth system" may be a cereal growth culture or may be a field containing planted cereal crops or cereal seeds. Similarly, a "turf grass growth system" may be 15 a turf grass growth culture or may be a field, lawn or golf course containing planted turf grass or turf grass seeds. It can serve as a safer alternative to synthetic herbicides now on the market. A primary object of the invention is to provide novel herbicidal compositions against both broadleaf, sedge and grassy weeds, which include but are not limited to Chenopodium album, Abutilon theophrasti, Helianthus annuus, Ambrosia artemesifolia, Amaranthus retroflexus, Convolvulus arvensis, Brassica 20 kaber, Taraxacum officinale, Solanum nigrum, Malva neglect, Setaria lutescens, Bromus tectorum, Poa annua, Poa pratensis, Lolium perenne L. var. Pace, Festuca arundinaceae Schreb. var. Aztec II, Anthem II, LS 1100, Echinochloa crus-galli, and particularly, Lambsquarter - Chenopodium album, Redroot Pigweed - Amaranthus retroflexus, Wild Mustard - Brassica kaber, Dandelion - Taraxacum officinale, and Black Nightshade - Solanum nigrum,that contains thaxtomin as an active ingredient. 25 Another object is to provide a safe, non-toxic herbicidal composition that does not harm cereal crops, pasture grass, Timothy grass or turf grass and a method that will not harm the environment. The above and other objects are accomplished by the present invention which is directed to herbicidal compositions containing at least one herbicidal agent, e.g., thaxtomin with optionally certain carriers to control the growth and germination of weeds in the cereal growth system and/or 30 turf grass growth system and/or Timothy grass growth system and/or pasture grass growth system. In particular, the invention is further directed to an herbicidal composition for use in modulating the germination and growth of monocotyledonous and/or dicotyledenous and/or sedge weeds in a cereal 2 WO 2010/078452 PCT/US2009/069856 growth system. In a particular embodiment, the cereal growth system is a non-rice cereal growth system comprising at least one herbicide in which said herbicide is thaxtomin. The compositions of the present invention may further comprise a carrier and/or diluent. In a particular embodiment, the composition is an aqueous composition. In another particular embodiment, the thaxtomin in the 5 composition is dissolved in a diluent comprising an organic solvent such as ethanol, isopropanol, or an aliphatic ketone such as acetone, methyl ethyl ketone, methyl isobutyl ketone. In a related aspect, the invention is directed to the use of at least one herbicidal agent, e.g., thaxtomin, in formulation of an herbicide for modulating monocotyledonous and/or dicotyledenous and/or sedge weeds a cereal growth system, e.g., a non-rice cereal growth system. Similarly, the 10 invention is directed to the use of at least one herbicidal agent in formulation of an herbicide for modulating monocotyledonous and/or dicotyledenous and/or sedge weeds in a turf grass growth system and/or Timothy grass growth system and/or pasture grass growth system, wherein at least one herbicidal agent is thaxtomin. The compositions of the present invention may comprise in addition to thaxtomin, at least 15 one or more herbicides. Thus the invention may comprise a thaxtomin and a chemical herbicide and/or bioherbicide. Compositions comprising thaxtomin and at least a second herbicide may be used in cereal growth systems (e.g., wheat, triticale, barley, oats, rye, corn, sorghum, sugarcane, rice or millet) and/or turf grass growth systems and/or Timothy grass growth systems and/or pasture grass growth systems. 20 Given that the invention is directed to the use of thaxtomin as a pre- or post-emergence herbicide, the invention is directed to a method for selectively modulating germination and growth of monocotyledonous, dicotyledonous and sedge weeds in a cereal crop growth system. In a particular embodiment, the cereal growth system is a non-rice cereal crop growth system comprising applying to said weeds or soil in said cereal crop growing system at least one herbicidal agent, wherein said 25 herbicidal agent is thaxtomin, in an amount of effective to modulate germination and growth of said weeds but not modulate growth of cereal crop in said cereal crop growth system. The cereal crop may include but is not limited to corn, wheat, triticale, barley, rye, oats, sorghum, sugarcane, and millet. The invention is further directed to a method for modulating germination and growth of monocotyledonous, dicotyledonous and sedge weeds in a turf, pasture and/or Timothy grass growth 30 system comprising applying to said weeds or soil in said turf grass growing system at least one herbicidal agent, wherein said herbicidal agent is thaxtomin, in an amount of effective to modulate growth of said weeds but not modulate germination and growth of turf grass in said turf grass growth 3 WO 2010/078452 PCT/US2009/069856 system, pasture grass in said pasture grass growth system and/or Timothy grass in said Timothy grass growth system. The turf grass may be selected from the group consisting of Festuca sp., Poa sp., Bromus sp., Lolium sp., Agrostis sp., Zoysia sp., Cynodon sp. Further, the invention is directed to a method for modulating germination and growth of 5 weeds selected from the group consisting of Chenopodium album, Abutilon theophrasti, Helianthus annuus, Ambrosia artemesifolia, Amaranthus retroflexus, Convolvulus arvensis, Brassica kaber, Taraxacum officinale, Solanum nigrum, Malva neglect,, Setaria lutescens, Bromus tectorum, Poa annua, Poa pratensis, Lolium perenne L. var. Pace, Festuca arundinaceae Schreb. var. Aztec II, Anthem II, LS1 100, Echinochloa crus-galli, comprising applying to said weeds or soil an amount of 10 thaxtomin or salt thereof effective to modulate said germination and growth of said weeds. The method of the present invention may also involve the use of at least a second herbicidal agent. The two herbicidal agents may be applied together in one formulation or separately in two formulations. Control of weeds can be achieved by using thaxtomin A in a tank mix or rotation with other herbicidally active compounds known to have good activity against grass weeds but no or low 15 phytotoxicity against cereal crops and/or turf grass and/or, pasture grass and/or Timothy grasses. In particular, the invention relates to a method for modulating growth of monocotyledonous, dicotyledonous and sedge weeds comprising applying to said weeds an amount of thaxtomin and amount of at least a second herbicidal agent to modulate growth of said weeds. The two herbicidal agents may be applied together in one formulation or separately in two formulations. The thaxtomin 20 and second herbicidal agent may be applied in a cereal growth system (e.g., wheat, triticale, barley, oats, rye, corn, sorghum, sugarcane, rice or millet) and/or turf grass growth system and/or pasture grass growth system and/or Timothy grass growth sysem. BRIEF DESCRIPTION OF THE FIGURES 25 Figure 1 shows the structure of Thaxtomin A. DETAILED DESCRIPTION OF THE INVENTION Where a range of values is provided, it is understood that each intervening value, to the tenth of the unit of the lower limit unless the context clearly dictates otherwise, between the upper and 30 lower limit of that range and any other stated or intervening value in that stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included in the smaller ranges is also encompassed within the invention, subject to any specifically 4 WO 2010/078452 PCT/US2009/069856 excluded limit in the stated range. Where the stated range includes one or both of the limits, ranges excluding either both of those included limits are also included in the invention. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. 5 Although any methods and materials similar or equivalent to those described herein can also be used in the practice or testing of the present invention, the preferred methods and materials are now described. It must be noted that as used herein and in the appended claims, the singular forms "a," "and" and "the" include plural references unless the context clearly dictates otherwise. 10 Thaxtomin utilized in this invention may be derived in fermentation of the following actinomycetes cultures: S. scabies - ATCC 49173 , S. acidiscabies - ATCC 49003 and BL37-EQ-010 - or it can be purchased from commercial sources. The thaxtomin utilized in the invention include but are not limited to agents described as cyclic dipeptides having the basic structure cyclo-(L-4-nitrotryptophyl-L-phenylalanyl). In 15 embodiments, suitable diketopiperazne moieties may be N-methylated, and include congeners carrying phenylalanyl alpha andring-carbon hydroxyl groups. The chemical in a particular embodiment comprises: NO2 O H R3 R6 N o N. N H R1 R5 )R2 O R4 20 wherein R 1 is methyl or H, R 2 is hydroxy or H, R 3 is methyl or H, R 4 is hydroxy or H, R 5 is hydroxy or H, R 6 is hydroxy or H, and combinations thereof. Non limiting examples of suitable thaxtomin is for use in accordance with the present invention include but are not limited to thaxtomin A, thaxtomin A ortho isomer, thaxtomin B, 25 thaxtomin C, hydroxythaxtomin C, thaxtomin A p-isomer, hydroxythaxtomin A and des-N-methylthaxtomin C and derivatives of any of these (See Figure 1). 5 WO 2010/078452 PCT/US2009/069856 The compositions of the present invention may be sprayed on the plant or applied to soil. Particular embodiments are described in the Examples, infra. These compositions may be in the form of dust, coarse dust, micro granules, granules, wettable powder, emulsifiable concentrate, liquid preparation, suspension concentrate, water degradable granules or oil suspension. 5 The compositions of the invention do comprise a carrier and/or diluent. The term, 'carrier' as used herein means an inert, organic or inorganic material, with which the active ingredient is mixed or formulated to facilitate its application to plant or other object to be treated, or its storage, transport and/or handling. Examples of diluents or carriers for the pre- and post-emergence herbicides include, but are not limited to, water, milk, ethanol, mineral oil, glycerol. 10 The compositions of the present invention may comprise at least two herbicidal agents. One herbicidal agent is thaxtomin set forth above. It may be present in one embodiment thaxtomin is present in an amount ranging from about 0.01 to about 5.0 mg/mL. The other herbicidal agent may be a bioherbicide and/or a chemical herbicide. The bioherbicide may be selected from the group consisting of clove, cinnamon, lemongrass, citrus oils, orange peel oil, tentoxin, cornexistin, 15 AAL-toxin, leptospermone, sarmentine, momilactone B, sorgoleone, ascaulatoxin and ascaulatoxin aglycone. In a particular embodiment, the composition may comprise thaxtomin, lemongrass oil and optionally a surfactant and/or vegetable oil. In another embodiment, the composition may comprise thaxtomin, sarmentine and optionally a nonionic surfactant and/or vegetable oil. The bioherbicide such as lemongrass oil or sarmentine may be present in an amount ranging from about 0.1 mg/mL to 20 about 50 mg/mL and more preferably between about 0.5 mg/mL to about 10 mg/mL The chemical herbicide may be selected from the group consisting diflufenzopyr and salts thereof, dicamba and salts thereof, topramezone, tembotrione, S-metolachlor, atrazine, mesotrione, primisulfuron-methyl, 2,4- dichlorophenoxyacetic acid, nicosulfuron, thifensulfuron-methyl, asulam, metribuzin, diclofop-methyl, fluazifop, fenoxaprop-p-ethyl, asulam, oxyfluorfen, rimsulfuron, mecoprop, and 25 quinclorac, thiobencarb, clomazone, cyhalofop, propanil, bensulfuron-methyl, penoxsulam, triclopyr, imazethapyr, halosulfuron-methyl, pendimethalin, bispyribac-sodium, carfentrazone ethyl, sodium bentazon/sodium acifluorfen and orthosulfamuron. The chemical herbicide such as pendimethalin or clomazone may be present in a pre-emergent weed control application in an amount ranging from about 0.5 mg/mL to 15 mg/mL 30 and a chemical herbicide such as cyhalofop, S-metolachlor, bispyribac-sodium, penoxsulam in a post-emergent application from about 1 mg/mL to about 40 mg/mL and more particularly between about 15 mg/ml to about 35 mg/mL. The composition may further comprise an adjuvant which may 6 WO 2010/078452 PCT/US2009/069856 be vegetable oil comprising ethyl oleate, polyethylene dialkyl ester and ethoxylated nonylphenol. The composition may additionally comprise a surfactant to be used for the purpose of emulsification, dispersion, wetting, spreading, integration, disintegration control, stabilization of active ingredients, improvement of fluidity or rust inhibition. The choice of dispersing and emulsifying agents, such as 5 non-ionic, anionic, amphoteric and cationic dispersing and emulsifying agents, and the amount employed is determined by the nature of the composition and the ability of the agent to facilitate the dispersion of the herbicidal compositions of the present invention. For post-emergent formulations, the formulation components used may contain smectite clays, attapulgite clays and similar swelling clays, thickeners such as xanthan gums, gum Arabic and 10 other polysaccharide thickeners as well as dispersion stabilizers such as nonionic surfactants (for example polyoxyethylene (20) monolaurate or polysorbate 60 POE (20) sorbitan monostearate, ethylene glycol monostearate). The concentration of the clays may vary between about 0-2.5% w/w of the total formulation, the polysaccharide thickeners may range between about 0-0.5% w/w of the total formulation and the surfactants may range from about 0-5% w/w of the total formulation. 15 EXAMPLES The composition and method of the present invention will be further illustrated in the following, non-limiting Examples. The examples are illustrative of various embodiments only and do not limit the claimed invention regarding the materials, conditions, weight ratios, process parameters 20 and the like recited herein. EXAMPLE 1 In a pot study test in greenhouse conditions, 6-inch corn plants (Zea mays var. Sunglow) were sprayed with increasing concentrations of thaxtomin A mixed in a carrier 4% ethanol, 0.02 % 25 polysorbate 60 POE (20) sorbitan monostearate solution. The spraying solutions contained 0.125, 0.25, 0.5 and 1.0 mg thaxtomin A/mL, and the plants are sprayed until total coverage. Each treatment was done in three replicates, and a control solution consists of water with 4% ethanol and 0.02 % polysorbate 60 POE (20) sorbitan monostearate as a surfactant. Prior to and after treatments, plants are grown in a greenhouse under artificial lights (12-h light/dark cycle) at 25 0 C. 30 Plants are evaluated in one-week intervals starting at 7 days after treatment. The final evaluation is done three weeks after treatment, at which time point, no phytotoxicity is observed in any of the test plants even at the highest thaxtomin A concentration. 7 WO 2010/078452 PCT/US2009/069856 EXAMPLE 2 A pot study is conducted to test the phytotoxicity of thaxtomin A on corn (Zea mays var. Early Sunglow) and wheat (Triticum aestivum var. PR1404). To confirm the activity on broadleaf 5 weeds, pigweed (Amaranthus sp.) is planted in the same pot with either three corn or five wheat seeds, and sprayed simultaneously with the cereal test plants. The less than 3-inch tall plants grown under growth lights (12-h light/12-h dark) at 28 0 C are sprayed with thaxtomin A solutions derived from a liquid culture of S. acidiscabies containing 0.5, and 1.0 mg thaxtomin A per mL of solvent (4 % ethanol and 0.2% non-ionic surfactant). A solution of 4 % ethanol + 0.2% non-ionic surfactant 10 without thaxtomin A is used as a control treatment. Each treatment is conducted in three replicates. Treated plants are kept at 28 0 C under growth lights and observed at three time points - 7, 14 and 21 days after treatment - for visual symptoms of phytotoxicity on corn and wheat and % control of pigweed. At each time point, no symptoms of phytotoxicity are observed in the cereal plants treated 15 with thaxtomin A. The highest concentration of thaxtomin A (1.0 mg/mL) results in a complete control of pigweed grown in the same pots with corn and wheat. EXAMPLE 3 To test the phytotoxicity of thaxtomin A on sorghum plants, five seeds of sorghum (Sorghum 20 bicolor) are planted in each 4"x 4" plastic pot filled with soil. Plants were grown under optimal conditions in a greenhouse before and after treatment with solutions containing 0.5 and 1.0 mg thaxtomin A /mL. At the time of the treatment, the plants are about 3 inches tall. Each treatment is applied in three replicates, and a control treatment included plants treated with just the carrier (4% EtOH, 0.02 % polysorbate 60 POE (20) sorbitan monostearate). Evaluations for phytotoxicity are 25 performed at 7-day intervals starting one week after treatment. The last evaluation is performed three weeks after the treatment at which point, no phytotoxicity is observed in the treated plants in any treatment concentration. EXAMPLE 4 30 A strain of S. acidiscabies (ATCC-49003) is grown in oat bran broth for 5 days (25 0 C, 200rpm). The whole cell broth with thaxtomin A is extracted using XAD resin. The dried crude extract was resuspended in 4% ethanol and 0.02 % non-ionic surfactant at a concentration of 10 8 WO 2010/078452 PCT/US2009/069856 mg/mL, and the solutions with two different concentrations of thaxtomin A (0.5 and 1.0 mg/mL) are tested the following broadleaf weed species: Lambsquarter - Chenopodium album Velvetleaf - Abutilon theophrasti 5 Sunflower - Helianthus annuus Ragweed, Common - Ambrosia artemesifolia Pigweed , Redroot - Amaranthus retroflexus Bindweed, Common - Convolvulus arvensis Mustard, Wild - Brassica kaber 10 Dandelion - Taraxacum officinale Nightshade, Black - Solanum nigrum Mallow, Common - Malva neglecta and on the following grass weed species: Foxtail - Setaria lutescens 15 Brome, Downy - Bromus tectorum Bluegrass, Annual - Poa annua Bluegrass, Kentucky - Poa pratensis Ryegrass, Perennial - (Lolium perenne L. var. Pace) Fescue, Tall - (Festuca arundinaceae Schreb. var. Aztec II, Anthem II, LS1 100) 20 Barnyard Grass - Echinochloa crus-galli All plant species are tested in 4"x4" plastic pots in three replicates. The untreated control plants are sprayed with the carrier solution (4% Ethanol, 0.02% glycosperse) and the positive control plants with Roundup at a rate corresponding to 1 fl. oz/acre. Treated plants are kept in a greenhouse 25 under 12h light/12h dark conditions. Data for broadleaf species from weekly evaluations are presented in Table 1. 9 WO 2010/078452 PCT/US2009/069856 Table 1. Weed control efficacy of a S. acidiscabies extract containing thaxtomin A on different weed species. Rating scale: 0 - no control, 1 - 10 % control, 2 - 25% control, 3 5 50% control, 4 - 75% control, 5 - 100 % control. Weed UTC THAXTOMIN THAXTOMIN species SOLUTION SOLUTION 1.0 mg/mL 0.5 mg/mL 7DAYS 14DAYS 21DAYS 7DAYS 14DAYS 21DAYS 7DAYS 14DAYS 21DAYS I I I I II Dandelion 0.0 0.0 0.0 2.0 2.3 4.0 2.0 2.0 3.7 Nightshade 0.0 0.0 0.0 2.7 2.2 2.3 2.7 2.0 2.3 Lambsquarter 0.0 0.0 0.0 2.0 2.0 2.0 2.0 2.0 2.0 Ragweed 0.0 0.0 0.0 1.0 0.5 0.0 1.0 0.5 0.0 Velvetleaf 0.0 0.0 0.0 1.7 1.0 1.0 2.0 1.0 0.3 Bindweed 0.0 0.0 0.0 1.0 1.0 0.0 1.2 1.0 0.0 Mustard 0.0 0.0 0.0 3.3 4.0 4.5 3.5 2.8 3.5 Sunflower 0.0 0.0 0.0 1.0 2.0 0.5 1.0 1.7 0.5 Mallow 0.0 0.0 0.0 1.0 1.0 1.0 1.2 1.0 1.0 Pigweed 0.0 0.0 0.0 3.5 4.0 4.0 4.2 3.0 3.7 10 WO 2010/078452 PCT/US2009/069856 The extract from a bacterial culture of S. acidiscabies with a thaxtomin A concentration of 0.5 mg/mL or higher showed good efficacy (>50%) against at least three of the most common broadleaf weed species (dandelion, mustard and pigweed) in both cereal and turf growing systems. Control of some weeds such as Black nightshade and Common lambsquarter was not 5 complete but thaxtomin A even at the lower concentration (0.5 mg/mL) results in severe stunting of these weeds. In this same study, no adverse effects are observed in grass species treated with either 0.5 or 1.0 mg/mL thaxtomin A. In all tested grass species, no phytotoxic effects were visible at even the higher thaxtomin A concentration. 10 EXAMPLE 5 The combined effect of thaxtomin A and two commercial herbicides (Bipyribac-sodium formulated as Regiment and Lemongrass oil formulated as GreenMatch EX) on small-flower umbrella sedge and watergrass is tested in a field study using small (1- sq foot) plots. All single product treatments and tank mix combinations were sprayed at 57 gal per acre. Evaluation of % 15 control was done 14 days after treatment and the results are presented in Table 2 below. Means in each column marked with the same letter in Table 2 are not statistically different from each other at p<0.05 According to the results, lemongrass oil at 1.25% weight does not improve the efficacy of thaxtomin A (at 0.25 mg/mL) on sedge but it significantly increases the efficacy on grass 20 weeds such as watergrass (field test) and sprangletop (greenhouse test). 11 WO 2010/078452 PCT/US2009/069856 Table 2. Effect of thaxtomin A alone and in combination with bispyribac-sodium and lemongrass oil on two rice weeds, small-flower umbrella sedge and watergrass. Treatment Sedge control (%) Watergrass control (%) Thaxtomin 0.25 mg/mL 95a 5d Thaxtomin 0.5 mg/mL 100a 5d Bispyribac-sodium 87.5a 32.5a (12 g/acre) Bispyribac-sodium M (6g/acre) 47.5c 15c Bispyribac-sodium 1 + 67.5b 25ab Thaxtomin 0.5 mg/mL Bispyribac-sodium /+ 55bc 7.5c Thaxtomin 0.25 mg/mL Lemongrass oil 5 % 15d 10c Lemongrass oil 2.5% 12.5d 10c Lemongrass oil 1.25% 20d 5d Lemongrass oil 1.25% + 100a 10c Thax 0.25 mg/mL Lemongrass oil 1.25% + 100a 20b Thaxtomin 0.5 mg/mL 5 10 According to the results, lemongrass oil at 1.25% does not improve the efficacy of thaxtomin A (at 0.25 mg/mL) on sedge but it significantly increases the efficacy on grass weeds 12 WO 2010/078452 PCT/US2009/069856 such as watergrass (field test) and sprangletop. Thaxtomin A (at 0.5 mg/mL) improves the efficacy of an ALS inhibitor, bipyribac sodium; used at half label rate on both sedge and grasses. EXAMPLE 6 5 The efficacy of thaxtomin A derived from a liquid culture of S. acidiscabies is tested in a field study on rice using 4.9 sq-ft plots surrounded by a metal ring. Treatments with either thaxtomin A or thaxtomin A in combination with lemongrass oil (formulated as GreenMatch EX) or cyhalofop (formulated as Clincher CA) were done using a hand-held sprayer with a water volume corresponding to 57 gallons per acre. Rice (variety M209) was grown until maturity and 10 harvested by hand for yield and weed count assessment. Results of yield (kg/ha), and numbers of redstem, small-flower umbrella sedge, and sprangletop in each plot are presented in Table 3 below. Table 3. Effect of thaxtomin A alone and in combination with lemongrass oil and cyhalofop 15 on rice yield and weed control. Treatment Yield (kg/ha) # of redstem # of sedge # of sprangletop 1 7516b 10.3 0.8a 86.Oa 2 7876b 0.5b 1.Oa 76.Oa 3 9054ab 0.3b 0.5a 69.3a 4 11296a 12.8a 0.5a 4.Ob 1. UTC; 2. Thaxtomin A (180 g/acre); 3. Lemongrass oil 1.25% + thaxtomin A (90 g/acre); 4. Cyhalofop (half label rate; 52 g/acre) + thaxtomin A (90 g/acre) + veg oil 2.5 % 20 Means in each column marked with the same letter are not statistically different from each other at p<0.05. Results indicate that thaxtomin at 180 g/acre significantly reduced the number of sedges but had no effect on sprangletop or yield. When used at half rate (thaxtomin A 90 g/acre), a combination with lemongrass oil had better effect on sedges than a combination with cyhalofop 25 (used at half label rate 52 g/acre). Good grass weed (sprangletop) control is achieved when thaxtomin (90 g/acre) is combined with cyhalofop at half the label rate - this combination also improves the yield significantly. EXAMPLE 7 30 Cyhalofop (2-[4-(4-cyano-2-fluorophenoxy)phenoxy]propanoic acid, butyl ester) is also mixed together with adjuvant containing ethyl oleate, polyethylene dialky ester and ethoxylated nonylphenol (2.5 % v/v) and increasing concentrations of thaxtomin A (purified from the ATCC 13 WO 2010/078452 PCT/US2009/069856 strain 49003) at concentrations 0.1, 0.2 and 0.4 mg/ml. The concentrations of the 2-[4-(4-cyano -fluorophenoxy)phenoxy]propanoic acid, butyl ester before dilution are 29.6% (2.38 lb/gal) and 21.7% (2 lb/gal), respectively. The effect of these mixtures on the growth of common water plantain, red stem, smallflower sedge and sprangletop is determined in the greenhouse. Similarly, 5 rice plants of variety M104 are grown and tested for phytotoxic effects, and all plants are evaluated 7, 14, and 21 days after treatment. Results of from the study with cyhalofop formulated as Clincher CA at the 21-day evaluation point are presented in Table 4 below. Table 4. Effect of thaxtomin A alone and with cyhalofop on rice yield and weed control Clincher CA Redstem Waterplantain Sedge Sprangletop (6.5 oz/acre) + % control % control % control % control Thaxtomin A (mg/mL) UTC 0 0 0 0 0-nothxA 75 8 0 90 0.1 100 85 87 100 0.2 97 87 88 100 0.4 100 85 100 100 10 As a conclusion, Clincher CA (29.6% cyhalofop by weight) applied at half label rate (6.5 oz/acre) has good efficacy against grass weeds - not so good on broadleaves and poor on sedges. A combination of Clincher CA (cyhalofop) and thaxtomin A provides good control of all rice weeds tested in this study. Efficacy of thaxtomin A against grass weeds is substantially improved if 15 combined with Clincher. Combination of thaxtomin A with Clincher CA did not cause phytotoxicity on rice at any tested concentration. EXAMPLE 8 Penoxsulam(2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5c]pyrimidin-2-yl 20 )-6-trifluoromethyl)benzenesulfonamide) is mixed together with adjuvant containing ethyl oleate, polyethylene dialky ester and ethoxylated nonylphenol (2.5 % v/v) and increasing concentrations of thaxtomin A (purified from the ATCC strain 49003) at concentrations 0.1, 0.2 and 0.4 mg/ml. The concentrations of the 2-[4-(4-cyano- -fluorophenoxy)phenoxy]propanoic acid, butyl ester or 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5c]pyrimidin-2-yl)-6-trifluoromethyl)b 25 enzenesulfonamide before dilution are 29.6% (2.38 lb/gal) and 21.7% (2 lb/gal), respectively. The effect of these mixtures on the growth of common water plantain, red stem, smallflower sedge and sprangletop is determined in the greenhouse. Similarly, rice plants of variety M104 are grown and tested for phytotoxic effects, and all plants are evaluated 7, 14, and 21 days after treatment. 14 WO 2010/078452 PCT/US2009/069856 EXAMPLE 9 A strain of S. acidiscabies was grown in oat bran broth for 5 days (25'C, 200 rpm). The whole cell broth was extracted using XAD resin, and the dried crude extract was resuspended in 5 4% ethanol and 0.2 % non-ionic surfactant at a concentration of 10 mg/mL. The diluted extracts containing 0.2 and 0.4 mg thaxtomin A per mL were tested on three weed species (redstem; Ammania spp., smallflower umbrella sedge; Cyperus difformis and sprangletop: Leptochloa uninervia). Other treatments included sarmentine at 2.5 and 5.0 mg/mL, and a combination treatment containing 0.2 mg thaxtomin A and 2.5 mg sarmentine per mL. Each treatment was 10 applied in three replicates. Treated plants were kept in a greenhouse under 12h light/12h dark conditions. Results from an evaluation performed 25 days after treatment are presented in Table 5. Table 5. Efficacy of herbicidal treatments using thaxtomin A (0.2 and 0.4 mg/mL) or 15 sarmentine (2.5 or 5.0 mg/mL) alone or in combination (0.2 + 2.5 mg/mL) to control broadleaf, sedge and grass weeds in a greenhouse study. treatment Control of redstem Control of sedge Control of sprangletop (%) (%) (%) UTC Oa Ga Ga Thaxtomin A 0.2 mg/mL 5.Ob 48.3b 8.3ab Thaxtomin A 0.4 mg/mL 11.7c* 91.7d 10.0b Thaxtomin 0.2 + 11.7c* 61.7c 73.3c Sarmentine 2.5 mg/mL Sarmentine 2.5 mg/mL Oa 8.3a 80.Oc Sarmentine 5.0 mg/mL 2.5ab 6.7a 92.3d * stunted. In a column, Means followed by the same letter are not statistically different from each other at p< 0.05. 20 Thaxtomin A at the highest concentration of 0.4 mg/mL provides excellent control of sedge but poor control of the grass weed (sprangletop). When combined with sarmentine, the efficacy against grass weeds improves significantly. Also, efficacy against sedge is improved with the combination treatment compared with the single application of thaxtomin A alone at the 15 WO 2010/078452 PCT/US2009/069856 corresponding concentration. In this study, the control of the broadleaf weed (redstem) is poor with all treatments. Although this invention has been described with reference to specific embodiments, the details thereof are not to be construed as limiting, as it is obvious that one can use various 5 equivalents, changes and modifications and still be within the scope of the present invention. Various references are cited throughout this specification, each of which is incorporated herein by reference in its entirety. 16 WO 2010/078452 PCT/US2009/069856 Cited references: Beaus6jour, J., C. Goyer, et al. (1999). "Production of thaxtomin A by Streptomyces scabies strains in plant extract containing media." Can J Microbiol 45: 764-768. 5 Duke, S. 0., S. R. Baerson, et al. (2003). "United States Department of Agriculture-Agricultural Research Service research on natural products for pest management." Pest Manag Sci 59: 708-717. 10 Duke, S. 0., F. E. Dayan, et al. (2000). "Natural products as sources of herbicides: current status and future trends." Weed Research 40: 99-111. Fry, B. A. and R. Loria (2002). "Thaxtomin A: Evidence for a plant cell wall target." Physiological and Molecular Plant Pathology 60: 1-8. 15 Gerwick, B. C., P. R. Graupner, et al. (2005). Methylidene mevalonates and their use as herbicides. U. p. 7393812: 16. Healy, F. G., M. J. Wach, et al. (2000). "The txtAB genes of the plant pathogen Streptomyces 20 acidiscabies encode a peptidesynthetase required for phytotoxin thaxtomin A prodcution and pathogenicity." Molecular Microbiology 38: 794-804. Hiltunen, L. H., I. Laakso, et al. (2006). "Influence of thaxtomins in different combinations and concentrations on growth of micropropagated potato shoot cultures." J Agric Food Chem 54: 25 3372-3379. Hoagland, R. E. (2001). "Microbial allelochemicals and pathogens as bioherbicidal agents." Weed Technology 15: 835-857. 30 Kang, Y., S. Semones, et al. (2008). Methods of controlling algae with thaxtomin and thaxtomin compositions. USA, Novozymes Biologicals, Inc. King, R. R., C. H. Lawrence, et al. (1992). "Chemistry of phytotoxins associated with Streptomyces scabies, the causal organism of potato common scab." J. Agric. Food Chem 40: 35 834-837. 17 WO 2010/078452 PCT/US2009/069856 King, R. R., C. H. Lawrence, et al. (1989). "Isolation and characterization of phytotoxin associated with Streptomyces scabies." Journal of the Chemical Society, Chemical Communications 13: 849-850. 5 King, R. R., C. H. Lawrence, et al. (2003). "More chemistry of the thaxtomin phytotoxins." Phytochemistry 64: 1091-1096. King, R. R., C. H. Lawrence, et al. (2001). "Herbicidal properties of the thaxtomin group of 10 phytotoxins." J Agric Food Chem 49: 2298-2301. Loria, R., R. A. Bukhalid, et al. (1995). "Differential production of thaxtomins by pathogenic Streptomyces species in vitro " Phytopathology 85: 537-541. 15 20 18

Claims (18)

1. A method for modulating germination and growth of monocotyledonous, dicotyledonous 5 and sedge weeds in a non-rice cereal crop growth system and/or a turf grass growth system and/or Timothy grass growth system and/or pasture grass growth system, comprising applying to said weeds or soil in said growth system at least one herbicidal agent, wherein said herbicidal agent is thaxtomin, in an amount of effective to modulate growth of said weeds but not modulate growth of non-rice cereal crop in said non-rice cereal crop growth system and/or turf, pasture and/or 10 Timothy grass in said turf, pasture and/or Timothy grass growth system. .

2. The method according to claim 1, wherein said cereal crop is at least one of wheat, triticale, barley, oats, rye, corn, sorghum, sugarcane or millet. 15

3. The method according to claim 3, wherein said turf grass is selected from the group consisting of Festuca sp., Poa sp., Bromus sp., Lolium sp., Agrostis sp., Zoysia sp., Cynodon sp.

4.The method according to claims 1, wherein the thaxtomin has the following composition: NO2 O H R3 R6 N /N. N H R1 R 5 ) R2 ( 20 0R4 wherein R 1 is methyl or H, R 2 is hydroxy or H, R 3 is methyl or H, R 4 is hydroxy or H, R 5 is hydroxy or H, R 6 is hydroxy or H, and combinations thereof.

5. The method according to claims 1, wherein the weed is selected from the group consisting of 25 Chenopodium album, Abutilon theophrasti, Helianthus annuus, Ambrosia artemesifolia, Amaranthus retroflexus, Convolvulus arvensis, Brassica kaber, Taraxacum officinale, Solanum nigrum, Malva neglect,, Setaria lutescens, Bromus tectorum, Poa annua, Poa pratensis, Lolium perenne L. var. Pace, Festuca arundinaceae Schreb. var. Aztec II, Anthem II, LS1 100, Echinochloa crus-galli. 19 WO 2010/078452 PCT/US2009/069856

6. A method for modulating growth of monocotyledonous, dicotyledonous and sedge weeds and aquatic weeds selected from the group consisting of Ammania sp., Alisma plantago-aquatica, Cyperus sp., Leptochloa sp. comprising applying to said weeds or soil an amount of at least two 5 herbicidal agents effective to modulate growth of said weeds, wherein a first herbicidal agent is thaxtomin.

7. The method according to claim 6, wherein said monocotyledonous, dicotyledonous and sedge weeds are modulated in a cereal growth system. 10

8. The method according to claim 7, wherein the cereal growth system is a rice growth system.

9. The method according to claim 6, wherein said second herbicidal agent is a chemical herbicide or bioherbicide. 15

10. The method according to claim 6, wherein the second herbicidal agent is a chemical herbicide selected from the group consisting of diflufenzopyr and salts thereof, dicamba and salts thereof, topramezone, tembotrione, S-metolachlor, atrazine, mesotrione, primisulfuron-methyl, 2,4- dichlorophenoxyacetic acid, nicosulfuron, thifensulfuron-methyl, asulam, metribuzin, 20 diclofop-methyl, fluazifop, fenoxaprop-p-ethyl, asulam, oxyfluorfen, rimsulfuron, mecoprop, and quinclorac, thiobencarb, clomazone, cyhalofop, propanil, bensulfuron-methyl, penoxsulam, triclopyr, imazethapyr, halosulfuron-methyl, pendimethalin, bispyribac-sodium, carfentrazone ethyl, sodium bentazon/sodium acifluorfen, orthosulfamuron. 25

11. The method according to claim 6, wherein the second herbicidal agent is a bioherbicide selected from the group consisting of clove, cinnamon, lemongrass, citrus oils, orange peel oil, tentoxin, cornexistin, AAL-toxin, leptospermone, sarmentine, momilactone B, sorgoleone, ascaulatoxin and ascaulatoxin aglycone, 30

12. The method according to claim 6, wherein the first and second herbicidal agents are applied in rotation.

13. The method according to claim 6, wherein the first and second herbicidal agents are applied together. 35 20 WO 2010/078452 PCT/US2009/069856

14. A composition for use in modulating the germination or growth of monocotyledonous and/or dicotyledenous and/or sedge weeds in a non-rice cereal growth system and/or a turf, pasture and/or Timothy grass growth system comprising at least one herbicide in which said herbicide is thaxtomin. 5

15. An herbicidal composition comprising at least two herbicides wherein a first herbicide is thaxtomin and a second herbicide is a bioherbicide or chemical herbicide.

16. The composition according to claims 15, wherein said composition further comprises an 10 adjuvant, a non-ionic surfactant and/or an organic solvent.

17. The composition according to claims 15, wherein said composition further comprises a non-ionic surfactant and/or an aliphatic alcohol. 15

18. Use of at least one herbicidal agent in formulation of an herbicide for modulating monocotyledonous and/or dicotyledenous and/or sedge weeds in a non-rice cereal growth system and/or in a turf and/or pasture and/or Timothy grass growth system wherein at least one herbicidal agent is thaxtomin. 21