AU2009205643B2 - Variable current density single needle electroporation system and method - Google Patents
Variable current density single needle electroporation system and method Download PDFInfo
- Publication number
- AU2009205643B2 AU2009205643B2 AU2009205643A AU2009205643A AU2009205643B2 AU 2009205643 B2 AU2009205643 B2 AU 2009205643B2 AU 2009205643 A AU2009205643 A AU 2009205643A AU 2009205643 A AU2009205643 A AU 2009205643A AU 2009205643 B2 AU2009205643 B2 AU 2009205643B2
- Authority
- AU
- Australia
- Prior art keywords
- electrode
- tissue
- elongate
- ring
- needle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000004520 electroporation Methods 0.000 title claims abstract description 102
- 238000000034 method Methods 0.000 title claims description 56
- 235000012489 doughnuts Nutrition 0.000 claims abstract description 25
- 230000001976 improved effect Effects 0.000 claims abstract description 4
- 210000004027 cell Anatomy 0.000 claims description 67
- 238000002347 injection Methods 0.000 claims description 62
- 239000007924 injection Substances 0.000 claims description 62
- 239000000126 substance Substances 0.000 claims description 60
- 230000001225 therapeutic effect Effects 0.000 claims description 46
- 239000003990 capacitor Substances 0.000 claims description 45
- 239000012530 fluid Substances 0.000 claims description 33
- 241001465754 Metazoa Species 0.000 claims description 20
- 238000004891 communication Methods 0.000 claims description 19
- 238000003780 insertion Methods 0.000 claims description 15
- 230000037431 insertion Effects 0.000 claims description 15
- 230000000149 penetrating effect Effects 0.000 claims description 15
- 239000000758 substrate Substances 0.000 claims description 15
- 238000001727 in vivo Methods 0.000 claims description 14
- 238000009413 insulation Methods 0.000 claims description 12
- 230000001953 sensory effect Effects 0.000 claims description 9
- 241000124008 Mammalia Species 0.000 claims description 8
- 230000006870 function Effects 0.000 claims description 8
- 238000005259 measurement Methods 0.000 claims description 8
- 239000004593 Epoxy Substances 0.000 claims description 7
- 230000033001 locomotion Effects 0.000 claims description 5
- 229910021645 metal ion Inorganic materials 0.000 claims description 5
- 210000002569 neuron Anatomy 0.000 claims description 5
- 239000004033 plastic Substances 0.000 claims description 5
- 239000011248 coating agent Substances 0.000 claims description 4
- 238000000576 coating method Methods 0.000 claims description 4
- 239000012811 non-conductive material Substances 0.000 claims description 4
- 210000000170 cell membrane Anatomy 0.000 claims description 3
- -1 paralene Polymers 0.000 claims description 3
- 230000003247 decreasing effect Effects 0.000 claims description 2
- 230000005465 channeling Effects 0.000 claims 2
- 239000012212 insulator Substances 0.000 claims 2
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims 1
- 230000004936 stimulating effect Effects 0.000 claims 1
- 230000035807 sensation Effects 0.000 abstract description 15
- 230000009467 reduction Effects 0.000 abstract description 3
- 230000008685 targeting Effects 0.000 abstract description 3
- 210000001519 tissue Anatomy 0.000 description 288
- 210000003205 muscle Anatomy 0.000 description 58
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 37
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 37
- 239000005090 green fluorescent protein Substances 0.000 description 37
- 150000007523 nucleic acids Chemical class 0.000 description 32
- 230000014509 gene expression Effects 0.000 description 27
- 102000039446 nucleic acids Human genes 0.000 description 26
- 108020004707 nucleic acids Proteins 0.000 description 26
- 108090000765 processed proteins & peptides Proteins 0.000 description 26
- 210000003491 skin Anatomy 0.000 description 26
- 102000004196 processed proteins & peptides Human genes 0.000 description 25
- 108090000623 proteins and genes Proteins 0.000 description 25
- 108020004414 DNA Proteins 0.000 description 23
- 229920001184 polypeptide Polymers 0.000 description 23
- 239000000463 material Substances 0.000 description 20
- 230000028993 immune response Effects 0.000 description 19
- 108091033319 polynucleotide Proteins 0.000 description 17
- 102000040430 polynucleotide Human genes 0.000 description 17
- 239000002157 polynucleotide Substances 0.000 description 17
- 230000001105 regulatory effect Effects 0.000 description 17
- 241000283973 Oryctolagus cuniculus Species 0.000 description 16
- 239000003795 chemical substances by application Substances 0.000 description 14
- 239000003814 drug Substances 0.000 description 14
- 230000005684 electric field Effects 0.000 description 14
- 230000001939 inductive effect Effects 0.000 description 13
- 239000013612 plasmid Substances 0.000 description 13
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 12
- 238000002474 experimental method Methods 0.000 description 12
- 108020004999 messenger RNA Proteins 0.000 description 12
- 238000011282 treatment Methods 0.000 description 12
- 230000007246 mechanism Effects 0.000 description 10
- 229910001220 stainless steel Inorganic materials 0.000 description 10
- 239000010935 stainless steel Substances 0.000 description 10
- 230000000692 anti-sense effect Effects 0.000 description 9
- 230000000875 corresponding effect Effects 0.000 description 9
- 238000013461 design Methods 0.000 description 9
- 210000003314 quadriceps muscle Anatomy 0.000 description 9
- 238000010186 staining Methods 0.000 description 9
- 229940124597 therapeutic agent Drugs 0.000 description 9
- 102000053642 Catalytic RNA Human genes 0.000 description 8
- 108090000994 Catalytic RNA Proteins 0.000 description 8
- 210000004207 dermis Anatomy 0.000 description 8
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 8
- 229910052737 gold Inorganic materials 0.000 description 8
- 239000010931 gold Substances 0.000 description 8
- 108091092562 ribozyme Proteins 0.000 description 8
- 238000004364 calculation method Methods 0.000 description 7
- 210000000663 muscle cell Anatomy 0.000 description 7
- 238000013519 translation Methods 0.000 description 7
- 210000000577 adipose tissue Anatomy 0.000 description 6
- 230000008901 benefit Effects 0.000 description 6
- 230000007423 decrease Effects 0.000 description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 230000009977 dual effect Effects 0.000 description 6
- 230000002500 effect on skin Effects 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 239000013598 vector Substances 0.000 description 6
- 108091028043 Nucleic acid sequence Proteins 0.000 description 5
- 238000003491 array Methods 0.000 description 5
- 150000001875 compounds Chemical class 0.000 description 5
- 208000035475 disorder Diseases 0.000 description 5
- 238000009826 distribution Methods 0.000 description 5
- 229940079593 drug Drugs 0.000 description 5
- 210000004209 hair Anatomy 0.000 description 5
- 244000144980 herd Species 0.000 description 5
- 229910052751 metal Inorganic materials 0.000 description 5
- 239000002184 metal Substances 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 230000004048 modification Effects 0.000 description 5
- 238000012986 modification Methods 0.000 description 5
- 230000035515 penetration Effects 0.000 description 5
- 230000004044 response Effects 0.000 description 5
- 238000002560 therapeutic procedure Methods 0.000 description 5
- 229960005486 vaccine Drugs 0.000 description 5
- 210000002268 wool Anatomy 0.000 description 5
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 4
- 108091030071 RNAI Proteins 0.000 description 4
- 108091027967 Small hairpin RNA Proteins 0.000 description 4
- 108020004459 Small interfering RNA Proteins 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 241000700605 Viruses Species 0.000 description 4
- 239000000427 antigen Substances 0.000 description 4
- 102000036639 antigens Human genes 0.000 description 4
- 108091007433 antigens Proteins 0.000 description 4
- 230000002950 deficient Effects 0.000 description 4
- 230000009368 gene silencing by RNA Effects 0.000 description 4
- 230000002503 metabolic effect Effects 0.000 description 4
- 150000001455 metallic ions Chemical class 0.000 description 4
- 239000002679 microRNA Substances 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 239000005060 rubber Substances 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 239000002924 silencing RNA Substances 0.000 description 4
- 239000004055 small Interfering RNA Substances 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 231100000331 toxic Toxicity 0.000 description 4
- 230000002588 toxic effect Effects 0.000 description 4
- 238000013518 transcription Methods 0.000 description 4
- 230000035897 transcription Effects 0.000 description 4
- 102000004127 Cytokines Human genes 0.000 description 3
- 108090000695 Cytokines Proteins 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 3
- 108010074338 Lymphokines Proteins 0.000 description 3
- 102000008072 Lymphokines Human genes 0.000 description 3
- 108700011259 MicroRNAs Proteins 0.000 description 3
- 230000003213 activating effect Effects 0.000 description 3
- 239000002246 antineoplastic agent Substances 0.000 description 3
- 230000033228 biological regulation Effects 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 230000001413 cellular effect Effects 0.000 description 3
- 230000001276 controlling effect Effects 0.000 description 3
- 230000001419 dependent effect Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000005611 electricity Effects 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 230000036039 immunity Effects 0.000 description 3
- 230000003053 immunization Effects 0.000 description 3
- 230000002062 proliferating effect Effects 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- 230000001177 retroviral effect Effects 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 230000008093 supporting effect Effects 0.000 description 3
- 230000007704 transition Effects 0.000 description 3
- 239000011800 void material Substances 0.000 description 3
- 108020005544 Antisense RNA Proteins 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 2
- 102000055006 Calcitonin Human genes 0.000 description 2
- 108060001064 Calcitonin Proteins 0.000 description 2
- 241000283707 Capra Species 0.000 description 2
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 2
- 102100022641 Coagulation factor IX Human genes 0.000 description 2
- 102000007644 Colony-Stimulating Factors Human genes 0.000 description 2
- 108010071942 Colony-Stimulating Factors Proteins 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- 241000283086 Equidae Species 0.000 description 2
- 108010076282 Factor IX Proteins 0.000 description 2
- 108010054218 Factor VIII Proteins 0.000 description 2
- 102000001690 Factor VIII Human genes 0.000 description 2
- 101150066002 GFP gene Proteins 0.000 description 2
- 241000713813 Gibbon ape leukemia virus Species 0.000 description 2
- 241000713858 Harvey murine sarcoma virus Species 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- 102000006992 Interferon-alpha Human genes 0.000 description 2
- 108010047761 Interferon-alpha Proteins 0.000 description 2
- 102000003996 Interferon-beta Human genes 0.000 description 2
- 108090000467 Interferon-beta Proteins 0.000 description 2
- 102000008070 Interferon-gamma Human genes 0.000 description 2
- 108010074328 Interferon-gamma Proteins 0.000 description 2
- 108010063738 Interleukins Proteins 0.000 description 2
- 102000015696 Interleukins Human genes 0.000 description 2
- 102000004083 Lymphotoxin-alpha Human genes 0.000 description 2
- 108090000542 Lymphotoxin-alpha Proteins 0.000 description 2
- 241000713869 Moloney murine leukemia virus Species 0.000 description 2
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 description 2
- 241001529936 Murinae Species 0.000 description 2
- 108091061960 Naked DNA Proteins 0.000 description 2
- 102000003982 Parathyroid hormone Human genes 0.000 description 2
- 108090000445 Parathyroid hormone Proteins 0.000 description 2
- 241001494479 Pecora Species 0.000 description 2
- 241000714474 Rous sarcoma virus Species 0.000 description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 230000003416 augmentation Effects 0.000 description 2
- 239000003114 blood coagulation factor Substances 0.000 description 2
- 229940019700 blood coagulation factors Drugs 0.000 description 2
- 229960004015 calcitonin Drugs 0.000 description 2
- BBBFJLBPOGFECG-VJVYQDLKSA-N calcitonin Chemical compound N([C@H](C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(N)=O)C(C)C)C(=O)[C@@H]1CSSC[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1 BBBFJLBPOGFECG-VJVYQDLKSA-N 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 229910052804 chromium Inorganic materials 0.000 description 2
- 239000011651 chromium Substances 0.000 description 2
- 230000007012 clinical effect Effects 0.000 description 2
- 239000003184 complementary RNA Substances 0.000 description 2
- 239000004020 conductor Substances 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 229940127089 cytotoxic agent Drugs 0.000 description 2
- 241001493065 dsRNA viruses Species 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229960004222 factor ix Drugs 0.000 description 2
- 229960000301 factor viii Drugs 0.000 description 2
- 229940044627 gamma-interferon Drugs 0.000 description 2
- 238000001415 gene therapy Methods 0.000 description 2
- 102000034356 gene-regulatory proteins Human genes 0.000 description 2
- 108091006104 gene-regulatory proteins Proteins 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 230000002163 immunogen Effects 0.000 description 2
- 239000000367 immunologic factor Substances 0.000 description 2
- 238000011065 in-situ storage Methods 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 229940047122 interleukins Drugs 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 210000002540 macrophage Anatomy 0.000 description 2
- 230000003211 malignant effect Effects 0.000 description 2
- 230000005012 migration Effects 0.000 description 2
- 238000013508 migration Methods 0.000 description 2
- 229910052750 molybdenum Inorganic materials 0.000 description 2
- 239000011733 molybdenum Substances 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 210000005036 nerve Anatomy 0.000 description 2
- 230000007383 nerve stimulation Effects 0.000 description 2
- 229910052759 nickel Inorganic materials 0.000 description 2
- 239000000199 parathyroid hormone Substances 0.000 description 2
- 229960001319 parathyroid hormone Drugs 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 239000000813 peptide hormone Substances 0.000 description 2
- 235000015277 pork Nutrition 0.000 description 2
- 229910052710 silicon Inorganic materials 0.000 description 2
- 239000010703 silicon Substances 0.000 description 2
- 210000002363 skeletal muscle cell Anatomy 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 230000009885 systemic effect Effects 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 108020004491 Antisense DNA Proteins 0.000 description 1
- 102100024003 Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 Human genes 0.000 description 1
- 208000023275 Autoimmune disease Diseases 0.000 description 1
- 241000714235 Avian retrovirus Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 108010039209 Blood Coagulation Factors Proteins 0.000 description 1
- 102000015081 Blood Coagulation Factors Human genes 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 101100314454 Caenorhabditis elegans tra-1 gene Proteins 0.000 description 1
- 102000019034 Chemokines Human genes 0.000 description 1
- 108010012236 Chemokines Proteins 0.000 description 1
- 102100023804 Coagulation factor VII Human genes 0.000 description 1
- 230000007023 DNA restriction-modification system Effects 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000003951 Erythropoietin Human genes 0.000 description 1
- 108090000394 Erythropoietin Proteins 0.000 description 1
- 108010023321 Factor VII Proteins 0.000 description 1
- 108700028146 Genetic Enhancer Elements Proteins 0.000 description 1
- 102100041003 Glutamate carboxypeptidase 2 Human genes 0.000 description 1
- 108010051696 Growth Hormone Proteins 0.000 description 1
- 101000892862 Homo sapiens Glutamate carboxypeptidase 2 Proteins 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108010064593 Intercellular Adhesion Molecule-1 Proteins 0.000 description 1
- 108010064600 Intercellular Adhesion Molecule-3 Proteins 0.000 description 1
- 102100037877 Intercellular adhesion molecule 1 Human genes 0.000 description 1
- 102100037872 Intercellular adhesion molecule 2 Human genes 0.000 description 1
- 101710148794 Intercellular adhesion molecule 2 Proteins 0.000 description 1
- 102100037871 Intercellular adhesion molecule 3 Human genes 0.000 description 1
- 108010002352 Interleukin-1 Proteins 0.000 description 1
- 102000013462 Interleukin-12 Human genes 0.000 description 1
- 108010065805 Interleukin-12 Proteins 0.000 description 1
- 108010002350 Interleukin-2 Proteins 0.000 description 1
- 102000000588 Interleukin-2 Human genes 0.000 description 1
- YQEZLKZALYSWHR-UHFFFAOYSA-N Ketamine Chemical compound C=1C=CC=C(Cl)C=1C1(NC)CCCCC1=O YQEZLKZALYSWHR-UHFFFAOYSA-N 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- 102100034256 Mucin-1 Human genes 0.000 description 1
- 108010008707 Mucin-1 Proteins 0.000 description 1
- 208000004221 Multiple Trauma Diseases 0.000 description 1
- 102000007474 Multiprotein Complexes Human genes 0.000 description 1
- 108010085220 Multiprotein Complexes Proteins 0.000 description 1
- 206010028851 Necrosis Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 102100021768 Phosphoserine aminotransferase Human genes 0.000 description 1
- 108010072866 Prostate-Specific Antigen Proteins 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 102100038803 Somatotropin Human genes 0.000 description 1
- 241000251131 Sphyrna Species 0.000 description 1
- 101000874347 Streptococcus agalactiae IgA FC receptor Proteins 0.000 description 1
- 230000024932 T cell mediated immunity Effects 0.000 description 1
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 1
- 206010046865 Vaccinia virus infection Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 102000025171 antigen binding proteins Human genes 0.000 description 1
- 108091000831 antigen binding proteins Proteins 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 229940041181 antineoplastic drug Drugs 0.000 description 1
- 239000003816 antisense DNA Substances 0.000 description 1
- 230000003190 augmentative effect Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 239000000560 biocompatible material Substances 0.000 description 1
- 230000008236 biological pathway Effects 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 230000036755 cellular response Effects 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 230000008045 co-localization Effects 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 230000001010 compromised effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 239000000412 dendrimer Substances 0.000 description 1
- 229920000736 dendritic polymer Polymers 0.000 description 1
- 230000003292 diminished effect Effects 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000009510 drug design Methods 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 239000003822 epoxy resin Substances 0.000 description 1
- 229940105423 erythropoietin Drugs 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 229940012413 factor vii Drugs 0.000 description 1
- 230000009969 flowable effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 208000002672 hepatitis B Diseases 0.000 description 1
- 230000028996 humoral immune response Effects 0.000 description 1
- 230000008348 humoral response Effects 0.000 description 1
- 230000002519 immonomodulatory effect Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 238000002847 impedance measurement Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 239000012678 infectious agent Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000007972 injectable composition Substances 0.000 description 1
- 239000012774 insulation material Substances 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000007918 intramuscular administration Methods 0.000 description 1
- 238000001990 intravenous administration Methods 0.000 description 1
- 238000010253 intravenous injection Methods 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 229960003299 ketamine Drugs 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 206010025135 lupus erythematosus Diseases 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000007257 malfunction Effects 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 230000000873 masking effect Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 108091070501 miRNA Proteins 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 239000004005 microsphere Substances 0.000 description 1
- 239000003607 modifier Substances 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 238000011587 new zealand white rabbit Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000037368 penetrate the skin Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000008177 pharmaceutical agent Substances 0.000 description 1
- 239000008194 pharmaceutical composition Substances 0.000 description 1
- 229920001481 poly(stearyl methacrylate) Polymers 0.000 description 1
- 229920000647 polyepoxide Polymers 0.000 description 1
- 108040000983 polyphosphate:AMP phosphotransferase activity proteins Proteins 0.000 description 1
- OXCMYAYHXIHQOA-UHFFFAOYSA-N potassium;[2-butyl-5-chloro-3-[[4-[2-(1,2,4-triaza-3-azanidacyclopenta-1,4-dien-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol Chemical compound [K+].CCCCC1=NC(Cl)=C(CO)N1CC1=CC=C(C=2C(=CC=CC=2)C2=N[N-]N=N2)C=C1 OXCMYAYHXIHQOA-UHFFFAOYSA-N 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 238000003259 recombinant expression Methods 0.000 description 1
- 239000012858 resilient material Substances 0.000 description 1
- 230000000284 resting effect Effects 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 210000003705 ribosome Anatomy 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 210000001044 sensory neuron Anatomy 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 238000004513 sizing Methods 0.000 description 1
- 210000002027 skeletal muscle Anatomy 0.000 description 1
- 230000008591 skin barrier function Effects 0.000 description 1
- 210000004927 skin cell Anatomy 0.000 description 1
- 210000000329 smooth muscle myocyte Anatomy 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000011287 therapeutic dose Methods 0.000 description 1
- 210000003813 thumb Anatomy 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 102000003390 tumor necrosis factor Human genes 0.000 description 1
- 241000701161 unidentified adenovirus Species 0.000 description 1
- 241001529453 unidentified herpesvirus Species 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 238000009827 uniform distribution Methods 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
- 208000007089 vaccinia Diseases 0.000 description 1
- 239000013603 viral vector Substances 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
- A61N1/00—Electrotherapy; Circuits therefor
- A61N1/18—Applying electric currents by contact electrodes
- A61N1/32—Applying electric currents by contact electrodes alternating or intermittent currents
- A61N1/327—Applying electric currents by contact electrodes alternating or intermittent currents for enhancing the absorption properties of tissue, e.g. by electroporation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
- A61N1/00—Electrotherapy; Circuits therefor
- A61N1/18—Applying electric currents by contact electrodes
- A61N1/32—Applying electric currents by contact electrodes alternating or intermittent currents
- A61N1/325—Applying electric currents by contact electrodes alternating or intermittent currents for iontophoresis, i.e. transfer of media in ionic state by an electromotoric force into the body
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Radiology & Medical Imaging (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Molecular Biology (AREA)
- Electromagnetism (AREA)
- Cell Biology (AREA)
- Sustainable Development (AREA)
- Plant Pathology (AREA)
- Electrotherapy Devices (AREA)
- Media Introduction/Drainage Providing Device (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US1177208P | 2008-01-17 | 2008-01-17 | |
| US61/011,772 | 2008-01-17 | ||
| PCT/US2009/000273 WO2009091578A1 (en) | 2008-01-17 | 2009-01-16 | Variable current density single needle electroporation system and method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2009205643A1 AU2009205643A1 (en) | 2009-07-23 |
| AU2009205643B2 true AU2009205643B2 (en) | 2014-05-29 |
Family
ID=40885604
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2009205643A Active AU2009205643B2 (en) | 2008-01-17 | 2009-01-16 | Variable current density single needle electroporation system and method |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US10610684B2 (enExample) |
| EP (3) | EP3593855B1 (enExample) |
| JP (2) | JP2011509743A (enExample) |
| KR (1) | KR101642523B1 (enExample) |
| CN (1) | CN101939047B (enExample) |
| AU (1) | AU2009205643B2 (enExample) |
| CA (2) | CA2710408C (enExample) |
| DK (1) | DK2240238T3 (enExample) |
| ES (1) | ES2739877T3 (enExample) |
| WO (1) | WO2009091578A1 (enExample) |
Families Citing this family (75)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9125981B2 (en) * | 2009-02-21 | 2015-09-08 | Incumed, Llc | Fluid cartridges including a power source and partially implantable medical devices for use with same |
| US9370619B2 (en) * | 2009-02-21 | 2016-06-21 | Incumed, Llc | Partially implantable medical devices and delivery/manifold tube for use with same |
| US9370618B2 (en) * | 2009-02-21 | 2016-06-21 | Incumed, Llc | Partially implantable medical devices and methods |
| CN101829396B (zh) * | 2009-03-27 | 2013-01-30 | 清华大学 | 微针阵列芯片及利用其的经皮给药贴剂及其制备方法 |
| US11097099B2 (en) * | 2010-03-01 | 2021-08-24 | Inovio Pharmaceuticals, Inc. | Multiple tissue layer electroporation applicator and device |
| KR101883715B1 (ko) * | 2010-04-28 | 2018-07-31 | 이노비오 파마수티컬즈, 인크. | 구강 점막 전기천공 장치 및 그 용도 |
| KR101242553B1 (ko) * | 2011-03-25 | 2013-03-25 | 한국과학기술원 | 전기침 플랫폼 |
| KR101238727B1 (ko) * | 2011-03-28 | 2013-03-04 | 한국과학기술원 | 전기침 플랫폼 |
| KR20140068000A (ko) | 2011-06-15 | 2014-06-05 | 크론테크 파마 아베 | 주사 바늘 및 장치 |
| KR102325369B1 (ko) * | 2011-06-28 | 2021-11-11 | 이노비오 파마수티컬즈, 인크. | 최소 침습 피부 전기천공 장치 |
| WO2013141503A1 (ko) * | 2012-03-19 | 2013-09-26 | Na Jongju | 피부 개선 장치 |
| KR101716345B1 (ko) * | 2012-06-18 | 2017-03-14 | 주식회사 이루다 | 고주파 피부치료장치 |
| KR101227224B1 (ko) * | 2012-06-26 | 2013-01-28 | 백성기 | 고주파 피부치료장치 |
| US8825229B2 (en) * | 2012-07-10 | 2014-09-02 | Khalid Hamad ALNAFISAH | Mobile tracking and subduing apparatus, method, and computer program product |
| US11259737B2 (en) * | 2012-11-06 | 2022-03-01 | Nuvasive, Inc. | Systems and methods for performing neurophysiologic monitoring during spine surgery |
| KR101405060B1 (ko) * | 2012-12-28 | 2014-06-10 | 김현철 | 전침 |
| EP2767307A1 (en) * | 2013-02-18 | 2014-08-20 | Eyevensys | Device for the treatment of an ocular disease |
| US9373269B2 (en) * | 2013-03-18 | 2016-06-21 | Lifescan Scotland Limited | Patch pump training device |
| US20160056020A1 (en) * | 2013-03-27 | 2016-02-25 | Washington State University | Systems and methods for treating material surfaces |
| CN103275874B (zh) * | 2013-06-08 | 2014-09-10 | 苏州文曲生物微系统有限公司 | 一种高密度分布式立体电极装置 |
| CN103701493B (zh) * | 2013-10-16 | 2016-04-06 | 中国电子科技集团公司第二十三研究所 | 海底有中继海缆传输系统单极缆单端供电的实现方法 |
| ES2583159B1 (es) * | 2015-02-17 | 2017-04-28 | Ionclinics And Deionic, S.L. | Dispositivo para el diagnóstico y regeneración inducida en tejidos mediante electrolisis percutánea terapéutica y electro-estimulación focalizada con aguja bipolar |
| KR102594062B1 (ko) | 2015-03-30 | 2023-10-25 | 세팔리 테크놀로지 에스피알엘 | 삼차 신경의 경피 전기 자극을 위한 장치 |
| US10390707B2 (en) * | 2015-05-11 | 2019-08-27 | Samsung Electronics Co., Ltd | Needle for biosensor and bio sensor including the same |
| EP3295600B1 (en) | 2015-05-12 | 2020-08-05 | LG Electronics Inc. | Method for performing a logical channel prioritization in a carrier aggregation with at least one scell operating in an unlicensed spectrum and a device therefor |
| WO2016193403A1 (en) * | 2015-06-02 | 2016-12-08 | Oblique Therapeutics Ab | Electroporation device |
| KR101788301B1 (ko) * | 2015-09-17 | 2017-10-20 | 주식회사 엘림텍 | 전기천공장치 및 그 제어방법 |
| WO2017047847A1 (ko) * | 2015-09-18 | 2017-03-23 | 심양수 | 심부 골막에만 열과 전기자극을 가하는 고주파 화침의 코팅방법 및 이에 의해 코팅된 심부 골막에만 열과 전기자극을 가하는 고주파 화침 |
| EP3393575B1 (en) | 2015-12-22 | 2024-01-10 | Inovio Pharmaceuticals, Inc. | Electroporation device having a battery pack with power switch |
| JP6292705B2 (ja) * | 2016-02-22 | 2018-03-14 | 公益財団法人東京都医学総合研究所 | エレクトロポレーション用電極 |
| WO2017160948A1 (en) * | 2016-03-15 | 2017-09-21 | The Trustees Of Columbia University In The City Of New York | Devices and methods for detecting penetration of a semi-permeable membrane |
| US10233419B2 (en) | 2016-06-30 | 2019-03-19 | Zymergen Inc. | Apparatuses and methods for electroporation |
| MX2019003335A (es) | 2016-09-23 | 2019-12-11 | Inovio Pharmaceuticals Inc | Método y dispositivo para una transfusión in vivo de tejido adiposo con invasión mínima usando electroporación. |
| DE202018001803U1 (de) | 2017-05-19 | 2018-06-27 | Cefaly Technology Sprl | Externe Trigeminusnervenstimulation für die Akutbehandlung von Migräneattacken |
| US11801364B2 (en) * | 2017-06-30 | 2023-10-31 | Avectas Limited | Electrospray catheter |
| CA3066253C (en) | 2017-06-30 | 2023-10-31 | Inscripta, Inc. | Automated cell processing methods, modules, instruments, and systems |
| EP3658220B1 (en) * | 2017-07-28 | 2021-07-07 | Scandinavian ChemoTech AB | A device, e.g. a dynamic electro enhanced pain control (deepc) device, for delivery of electrical pulses to a desired body part of a mammal |
| US10738327B2 (en) | 2017-08-28 | 2020-08-11 | Inscripta, Inc. | Electroporation cuvettes for automation |
| US10435713B2 (en) | 2017-09-30 | 2019-10-08 | Inscripta, Inc. | Flow through electroporation instrumentation |
| WO2019075380A1 (en) * | 2017-10-12 | 2019-04-18 | Northwestern University | TARGETED ADMINISTRATION OF BIOLOGICAL THERAPEUTIC AGENTS |
| EP3775159A4 (en) | 2018-03-29 | 2022-01-19 | Inscripta, Inc. | AUTOMATED REGULATION OF CELL GROWTH RATES FOR INDUCTION AND TRANSFORMATION |
| US10376889B1 (en) | 2018-04-13 | 2019-08-13 | Inscripta, Inc. | Automated cell processing instruments comprising reagent cartridges |
| US10858761B2 (en) | 2018-04-24 | 2020-12-08 | Inscripta, Inc. | Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells |
| WO2019209926A1 (en) | 2018-04-24 | 2019-10-31 | Inscripta, Inc. | Automated instrumentation for production of peptide libraries |
| US10526598B2 (en) | 2018-04-24 | 2020-01-07 | Inscripta, Inc. | Methods for identifying T-cell receptor antigens |
| WO2019237107A1 (en) | 2018-06-08 | 2019-12-12 | Mayo Foundation For Medical Education And Research | Dermatological electroporation devices and methods |
| CA3108767A1 (en) | 2018-06-30 | 2020-01-02 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
| US10752874B2 (en) | 2018-08-14 | 2020-08-25 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
| US11142740B2 (en) | 2018-08-14 | 2021-10-12 | Inscripta, Inc. | Detection of nuclease edited sequences in automated modules and instruments |
| US10532324B1 (en) | 2018-08-14 | 2020-01-14 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
| US11965154B2 (en) | 2018-08-30 | 2024-04-23 | Inscripta, Inc. | Detection of nuclease edited sequences in automated modules and instruments |
| AU2019358073B2 (en) * | 2018-10-10 | 2022-06-30 | Pulse Biosciences, Inc. | Treatment of dermal glands by the application of non-thermal energy |
| CN113613711B (zh) * | 2019-02-04 | 2024-09-17 | 新泽西州立罗格斯大学 | 使用微电极进行组织电转移的装置 |
| CN110221185B (zh) * | 2019-05-20 | 2024-06-14 | 广东电网有限责任公司 | 一种模拟高压电缆中间接头界面沿面放电试验装置 |
| US10907125B2 (en) | 2019-06-20 | 2021-02-02 | Inscripta, Inc. | Flow through electroporation modules and instrumentation |
| WO2020257395A1 (en) | 2019-06-21 | 2020-12-24 | Inscripta, Inc. | Genome-wide rationally-designed mutations leading to enhanced lysine production in e. coli |
| US10927385B2 (en) | 2019-06-25 | 2021-02-23 | Inscripta, Inc. | Increased nucleic-acid guided cell editing in yeast |
| US11624050B2 (en) * | 2019-08-21 | 2023-04-11 | Board Of Regents, The University Of Texas System | Microneedle array electroporation system for cell transfection |
| CN114828947A (zh) * | 2019-10-18 | 2022-07-29 | 新南创新私人有限公司 | 电转移治疗递送装置、系统和方法 |
| CN110742685B (zh) * | 2019-11-04 | 2024-12-31 | 浙江大学 | 一种新型纳秒脉冲安全电极 |
| US10689669B1 (en) | 2020-01-11 | 2020-06-23 | Inscripta, Inc. | Automated multi-module cell processing methods, instruments, and systems |
| CN111067789A (zh) * | 2020-01-15 | 2020-04-28 | 山东倍帮医疗科技有限公司 | 一种脉冲微电流直压微针装置 |
| KR20220133257A (ko) | 2020-01-27 | 2022-10-04 | 인스크립타 인코포레이티드 | 전기천공 모듈 및 기구 |
| PH12022552378A1 (en) * | 2020-03-20 | 2023-12-18 | Inovio Pharmaceuticals Inc | Vacuum-assisted electroporation devices, and related systems and methods |
| US20210332388A1 (en) | 2020-04-24 | 2021-10-28 | Inscripta, Inc. | Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells |
| US11787841B2 (en) | 2020-05-19 | 2023-10-17 | Inscripta, Inc. | Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli |
| EP4125664B1 (en) * | 2020-06-18 | 2023-11-29 | St. Jude Medical, Cardiology Division, Inc. | Device for delivering electroporation therapy |
| WO2022058830A1 (en) * | 2020-09-15 | 2022-03-24 | Cochlear Limited | Dynamic electroporation |
| MX2024000127A (es) * | 2021-06-30 | 2024-04-01 | Inovio Pharmaceuticals Inc | Dispositivos de inyección de vía lateral para su uso con electroporación y sistemas y métodos relacionados. |
| US20230001169A1 (en) * | 2021-06-30 | 2023-01-05 | Inovio Pharmaceuticals, Inc. | Electrode Arrays For Electroporation, and Related Systems and Methods |
| CN113974863B (zh) * | 2021-12-03 | 2023-06-30 | 郑州大学第一附属医院 | 一种乳腺肿瘤穿刺活检实施定位装置 |
| AU2023224287A1 (en) * | 2022-02-25 | 2024-10-03 | Inovio Pharmaceuticals, Inc. | Handheld electroporation devices, and related systems and methods |
| CA3249065A1 (en) * | 2022-05-16 | 2023-11-23 | Vector Surgical, Llc | INTRACELLULAR TREATMENT DEVICE AND ITS METHODS OF USE |
| US20240091513A1 (en) * | 2022-09-16 | 2024-03-21 | Inovio Pharmaceuticals, Inc. | Vacuum-Assisted Treatment Device, and Related Systems and Methods |
| WO2025074366A1 (en) * | 2023-10-04 | 2025-04-10 | Pollogen Ltd. | Devices and methods for treatment of tissue with manually driven electrodes |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050070841A1 (en) * | 2002-07-04 | 2005-03-31 | Inovio As | Electroporation device and injection apparatus |
| US20060084938A1 (en) * | 1998-07-13 | 2006-04-20 | Genetronics, Inc. | Enhanced delivery of naked DNA to skin by non-invasive in vivo electroporation |
Family Cites Families (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS52118987A (en) * | 1976-03-31 | 1977-10-05 | Takeshi Okamoto | Electric treating conductor |
| US4699880A (en) | 1984-09-25 | 1987-10-13 | Immunomedics, Inc. | Method of producing monoclonal anti-idiotype antibody |
| US4704692A (en) | 1986-09-02 | 1987-11-03 | Ladner Robert C | Computer based system and method for determining and displaying possible chemical structures for converting double- or multiple-chain polypeptides to single-chain polypeptides |
| US5389069A (en) * | 1988-01-21 | 1995-02-14 | Massachusetts Institute Of Technology | Method and apparatus for in vivo electroporation of remote cells and tissue |
| US5703055A (en) | 1989-03-21 | 1997-12-30 | Wisconsin Alumni Research Foundation | Generation of antibodies through lipid mediated DNA delivery |
| US5246437A (en) * | 1992-04-10 | 1993-09-21 | Abela George S | Cell treatment apparatus and method |
| FR2693112B1 (fr) | 1992-07-01 | 1994-09-02 | Raymond Denance | Dispositif électromécanique d'injection à usage médical et vétérinaire actionné par une gâchette. |
| US5702359A (en) * | 1995-06-06 | 1997-12-30 | Genetronics, Inc. | Needle electrodes for mediated delivery of drugs and genes |
| US5536267A (en) * | 1993-11-08 | 1996-07-16 | Zomed International | Multiple electrode ablation apparatus |
| IL108775A (en) * | 1994-02-25 | 2003-09-17 | Univ Ramot | Method for efficient incorporation of molecules into cells |
| US6041252A (en) | 1995-06-07 | 2000-03-21 | Ichor Medical Systems Inc. | Drug delivery system and method |
| US5873849A (en) | 1997-04-24 | 1999-02-23 | Ichor Medical Systems, Inc. | Electrodes and electrode arrays for generating electroporation inducing electrical fields |
| US6635027B1 (en) * | 1997-05-19 | 2003-10-21 | Micro Therepeutics, Inc. | Method and apparatus for intramural delivery of a substance |
| US6391005B1 (en) * | 1998-03-30 | 2002-05-21 | Agilent Technologies, Inc. | Apparatus and method for penetration with shaft having a sensor for sensing penetration depth |
| EP1100579B1 (en) * | 1998-07-13 | 2015-09-02 | Inovio Pharmaceuticals, Inc. | Skin and muscle-targeted gene therapy by pulsed electrical field |
| CA2347091A1 (en) * | 1998-10-15 | 2000-04-20 | Children's Medical Center Corporation | Method and apparatus for direct in vivo gene transfer by electrotransfecection |
| US6314316B1 (en) * | 1998-12-17 | 2001-11-06 | University Of South Florida | Nonpenetrating electroporation device and method |
| US6613026B1 (en) * | 1999-12-08 | 2003-09-02 | Scimed Life Systems, Inc. | Lateral needle-less injection apparatus and method |
| US7404815B2 (en) * | 2000-05-01 | 2008-07-29 | Lifescan, Inc. | Tissue ablation by shear force for sampling biological fluids and delivering active agents |
| EP1404214A2 (en) * | 2000-11-24 | 2004-04-07 | CKM Diagnostics, Inc. | Nerve stimulator output control needle with depth determination capability and method of use |
| US6969373B2 (en) * | 2001-04-13 | 2005-11-29 | Tricardia, Llc | Syringe system |
| US7127284B2 (en) * | 2001-06-11 | 2006-10-24 | Mercator Medsystems, Inc. | Electroporation microneedle and methods for its use |
| US6981971B2 (en) * | 2001-06-15 | 2006-01-03 | Diomed Inc. | Medical laser device |
| US7245963B2 (en) | 2002-03-07 | 2007-07-17 | Advisys, Inc. | Electrode assembly for constant-current electroporation and use |
| AU2003230601A1 (en) * | 2002-03-07 | 2003-09-22 | Instituto Di Ricerche Di Biologia Molecolare P. Angeletti S.P.A. | Clinical syringe with electrical stimulation aspects |
| US7328064B2 (en) * | 2002-07-04 | 2008-02-05 | Inovio As | Electroporation device and injection apparatus |
| US20060269531A1 (en) * | 2003-07-18 | 2006-11-30 | Eastern Virginia Medical School | Apparatus for generating electrical pulses and methods of using the same |
| US20050209625A1 (en) | 2004-03-02 | 2005-09-22 | Chan Frank A | Method and apparatus for electrical stimulation to enhance lancing device performance |
| WO2006084173A1 (en) * | 2005-02-04 | 2006-08-10 | The Johns Hopkins University | Electroporation gene therapy gun system |
| JP2007097686A (ja) * | 2005-09-30 | 2007-04-19 | Transcutaneous Technologies Inc | イオントフォレーシス装置および作用側電極構造体ユニット |
| JP4379624B2 (ja) * | 2006-02-06 | 2009-12-09 | 宏三 大塩 | 微弱電流活性具 |
-
2009
- 2009-01-16 JP JP2010543139A patent/JP2011509743A/ja not_active Withdrawn
- 2009-01-16 KR KR1020107018104A patent/KR101642523B1/ko active Active
- 2009-01-16 CA CA2710408A patent/CA2710408C/en active Active
- 2009-01-16 CN CN200980103021.3A patent/CN101939047B/zh active Active
- 2009-01-16 WO PCT/US2009/000273 patent/WO2009091578A1/en not_active Ceased
- 2009-01-16 CA CA3128936A patent/CA3128936A1/en active Pending
- 2009-01-16 EP EP19169658.2A patent/EP3593855B1/en active Active
- 2009-01-16 EP EP09702445.9A patent/EP2240238B1/en active Active
- 2009-01-16 ES ES09702445T patent/ES2739877T3/es active Active
- 2009-01-16 DK DK09702445.9T patent/DK2240238T3/da active
- 2009-01-16 EP EP23201397.9A patent/EP4328297A2/en not_active Withdrawn
- 2009-01-16 AU AU2009205643A patent/AU2009205643B2/en active Active
-
2010
- 2010-07-14 US US12/836,163 patent/US10610684B2/en active Active
-
2013
- 2013-12-06 JP JP2013252684A patent/JP5756165B2/ja active Active
-
2020
- 2020-02-28 US US16/804,707 patent/US20200289818A1/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060084938A1 (en) * | 1998-07-13 | 2006-04-20 | Genetronics, Inc. | Enhanced delivery of naked DNA to skin by non-invasive in vivo electroporation |
| US20050070841A1 (en) * | 2002-07-04 | 2005-03-31 | Inovio As | Electroporation device and injection apparatus |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2009091578A1 (en) | 2009-07-23 |
| ES2739877T3 (es) | 2020-02-04 |
| EP2240238A1 (en) | 2010-10-20 |
| CA2710408C (en) | 2023-10-31 |
| KR101642523B1 (ko) | 2016-07-25 |
| EP4328297A2 (en) | 2024-02-28 |
| EP2240238A4 (en) | 2013-01-23 |
| CA3128936A1 (en) | 2009-07-23 |
| AU2009205643A1 (en) | 2009-07-23 |
| EP2240238B1 (en) | 2019-04-17 |
| JP5756165B2 (ja) | 2015-07-29 |
| DK2240238T3 (da) | 2019-07-22 |
| HK1152000A1 (en) | 2012-02-17 |
| JP2011509743A (ja) | 2011-03-31 |
| JP2014054568A (ja) | 2014-03-27 |
| EP3593855A1 (en) | 2020-01-15 |
| US20200289818A1 (en) | 2020-09-17 |
| US10610684B2 (en) | 2020-04-07 |
| CN101939047A (zh) | 2011-01-05 |
| CN101939047B (zh) | 2014-04-16 |
| CA2710408A1 (en) | 2009-07-23 |
| US20110009807A1 (en) | 2011-01-13 |
| KR20100106583A (ko) | 2010-10-01 |
| EP3593855B1 (en) | 2023-10-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20200289818A1 (en) | Variable current density single needle electroporation system and method | |
| US20230001190A1 (en) | Device and method for single-needle in vivo electroporation | |
| US20080045880A1 (en) | Device and method for single-needle in vivo electroporation | |
| JP2003505114A (ja) | パルス電場による皮膚および筋肉を標的とした遺伝子治療 | |
| CN113613711B (zh) | 使用微电极进行组织电转移的装置 | |
| EP2148721B1 (en) | Device and method for single-needle in vivo electroporation | |
| HK1152000B (en) | Variable current density single needle electroporation system and method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGA | Letters patent sealed or granted (standard patent) |