AU2007345768B2 - Chimeric influenza virus-like particles - Google Patents
Chimeric influenza virus-like particles Download PDFInfo
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- AU2007345768B2 AU2007345768B2 AU2007345768A AU2007345768A AU2007345768B2 AU 2007345768 B2 AU2007345768 B2 AU 2007345768B2 AU 2007345768 A AU2007345768 A AU 2007345768A AU 2007345768 A AU2007345768 A AU 2007345768A AU 2007345768 B2 AU2007345768 B2 AU 2007345768B2
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- influenza virus
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Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US83420006P | 2006-07-27 | 2006-07-27 | |
| US60/834,200 | 2006-07-27 | ||
| US87892007P | 2007-01-05 | 2007-01-05 | |
| US60/878,920 | 2007-01-05 | ||
| PCT/US2007/016900 WO2008094197A2 (en) | 2006-07-27 | 2007-07-27 | Chimeric influenza virus-like particles |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2007345768A1 AU2007345768A1 (en) | 2008-08-07 |
| AU2007345768B2 true AU2007345768B2 (en) | 2013-08-01 |
Family
ID=39674641
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2007345768A Ceased AU2007345768B2 (en) | 2006-07-27 | 2007-07-27 | Chimeric influenza virus-like particles |
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|---|---|
| US (1) | US9439959B2 (enExample) |
| EP (1) | EP2044198A4 (enExample) |
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| AU (1) | AU2007345768B2 (enExample) |
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| WO (1) | WO2008094197A2 (enExample) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2657955A1 (en) * | 2006-07-27 | 2008-08-07 | Ligocyte Pharmaceuticals, Inc. | Chimeric virus-like particles |
| KR20170001720A (ko) | 2007-03-14 | 2017-01-04 | 다케다 백신즈 인코포레이티드 | 바이러스 유사 입자 정제 |
| CA2742295A1 (en) * | 2008-11-03 | 2010-06-03 | Ligocyte Pharmaceuticals, Inc. | Improved methods for isolating enveloped virus-based vlps free of infectious agents |
| KR20110092316A (ko) * | 2008-11-19 | 2011-08-17 | 래보래토리오 아비-멕스, 에스.에이. 데 씨.브이. | 재조합 불활성화 바이러스 벡터 백신 |
| US20120121710A1 (en) | 2009-03-27 | 2012-05-17 | Arizona Board Of Regents For And On Behalf Of Arizona State University | Mucosal Immunization |
| EP2413961B1 (en) * | 2009-03-31 | 2016-08-17 | Japan As Represented By The Director-General Of National Institute Of Infectious Diseases | Method for prophylaxis of influenza using vaccine for intranasal administration |
| JP2012525133A (ja) * | 2009-05-01 | 2012-10-22 | レッドバイオテック・アーゲー | 多重遺伝子ベクターによってコードされる組換えウイルス様粒子 |
| JP5886194B2 (ja) | 2009-07-02 | 2016-03-16 | マサチューセッツ インスティテュート オブ テクノロジー | インフルエンザ感染の診断および／または処置のための組成物および方法 |
| EP2496609A4 (en) * | 2009-11-03 | 2013-05-08 | Ligocyte Pharmaceuticals Inc | CHIMERIC RSV-F POLYPEPTIDE AND VLPS ON LENTIVIREN OR ALPHA RETROVIRUS GAG BASE |
| CA2816401C (en) | 2009-11-05 | 2017-07-18 | Novici Biotech Llc | Generation of antigenic virus-like particles through protein-protein linkages |
| EP2519539A4 (en) * | 2009-12-28 | 2013-11-13 | Ligocyte Pharmaceuticals Inc | METHODS FOR STABILIZING VIRUS-LIKE PARTICULATE SOLUTIONS BASED ON INFLUENZA-INFLUENZA-VIRUSED VIRUSES |
| NZ601609A (en) * | 2010-01-21 | 2014-08-29 | Univ Arkansas | Vaccine vectors and methods of enhancing immune responses |
| KR101866301B1 (ko) * | 2010-03-29 | 2018-06-11 | 인쎄름 (엥스띠뛰 나씨오날 드 라 쌍떼 에 드 라 흐쉐르슈 메디깔) | 하나 이상의 ｃｘｘｃ 모티프를 포함하는 폴리펩티드 및 이종 항원을 포함하는 약학 조성물 및 이의 용도 |
| WO2012040406A2 (en) | 2010-09-21 | 2012-03-29 | Massachusetts Institute Of Technology | Influenza treatment and/or characterization; human-adapted ha polypeptides; vaccines |
| CA2813078A1 (en) | 2010-10-04 | 2012-04-12 | Massachusetts Institute Of Technology | Hemagglutinin polypeptides, and reagents and methods relating thereto |
| JOP20130186B1 (ar) | 2012-06-22 | 2021-08-17 | Takeda Vaccines Montana Inc | تنقية الجزيئات الشبيهة بالفيروسات |
| JP2015015931A (ja) * | 2013-07-12 | 2015-01-29 | 株式会社Ｕｍｎファーマ | ウイルス様粒子を含む培養物の製造方法 |
| KR101745472B1 (ko) * | 2015-04-15 | 2017-06-12 | 대한민국 | 조류인플루엔자 바이러스의 세포 감염을 저해하는 물질을 선별하는 방법 |
| AU2017260323B2 (en) | 2016-05-03 | 2023-11-16 | The Board Of Trustees Of The University Of Arkansas | Yeast vaccine vector including immunostimulatory and antigenic polypeptides and methods of using the same |
| CN115746107A (zh) | 2016-06-03 | 2023-03-07 | 圣诺菲·帕斯图尔公司 | 经改造的流感血凝素多肽的修饰 |
| JPWO2021085650A1 (enExample) * | 2019-10-31 | 2021-05-06 | ||
| CN113827715B (zh) * | 2020-06-23 | 2024-07-05 | 普莱柯生物工程股份有限公司 | 一种抗h7亚型和h5亚型禽流感病毒的疫苗组合物、及其制备方法和应用 |
| CN113264989A (zh) * | 2021-05-17 | 2021-08-17 | 吉林大学 | 一种h9n2亚型禽流感嵌合病毒样颗粒制备方法及应用 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003066868A1 (en) * | 2002-02-04 | 2003-08-14 | Oxford Biomedica (Uk) Limited | Retroviral vectors pseudotyped with influenza virus hemagglutinin for gene delivery |
| WO2004042001A2 (en) * | 2002-05-17 | 2004-05-21 | Emory University | Virus-like particles, methods of preparation, and immonogenic compositions |
| US20050186621A1 (en) * | 2000-06-23 | 2005-08-25 | American Cyanamid Company | Assembly of wild-type and chimeric influenza virus-like particles (VLPs) |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6485729B1 (en) * | 1993-09-13 | 2002-11-26 | Protein Sciences Corporation | Neuraminidase-supplemented compositions |
| WO1998023735A1 (en) | 1996-11-26 | 1998-06-04 | Stressgen Biotechnologies Corp. | Immune responses using compositions containing stress proteins |
| US6099847A (en) | 1997-05-15 | 2000-08-08 | The United States Of America As Represented By The Department Of Health And Human Services | Chimeric Gag pseudovirions |
| GB9727262D0 (en) * | 1997-12-24 | 1998-02-25 | Smithkline Beecham Biolog | Vaccine |
| US6602705B1 (en) * | 1998-12-31 | 2003-08-05 | Chiron Corporation | Expression of HIV polypeptides and production of virus-like particles |
| US20040105871A1 (en) * | 2000-03-02 | 2004-06-03 | Robinson Harriet L. | Compositions and methods for generating an immune response |
| CN1468301A (zh) | 2000-06-23 | 2004-01-14 | 从邻近启动子的基因表达保护性抗原的呼吸道合胞病毒疫苗 | |
| DE60127675T2 (de) | 2000-06-28 | 2007-12-27 | Fuji Nihon Seito Corp. | Neuartige insulin synthase und verfahren zur herstellung von insulin durch anwendung derselben |
| EP1201750A1 (en) | 2000-10-26 | 2002-05-02 | Genopoietic | Synthetic viruses and uses thereof |
| AU2002309100A1 (en) | 2001-03-12 | 2002-11-18 | Proteologics, Ltd. | Compositions and methods for the modulation of viral maturation |
| EP1409694A4 (en) | 2001-07-05 | 2006-02-08 | Chiron Corp | POLYNUCLEOTIDES ENCODING ANTIGENIC POLYPEPTIDES OF HIV SUBTYPE B AND / OR SUBTYPE C, POLYPEPTIDES AND USES THEREOF |
| DE60323407D1 (de) * | 2002-05-16 | 2008-10-16 | Bavarian Nordic As | Fusionsprotein des regulatorische/akzessorische HIV Proteine |
| EP1576143B1 (en) * | 2002-05-17 | 2010-11-24 | Emory University | Virus-like particles, methods of preparation, and immonogenic compositions |
| US9045727B2 (en) | 2002-05-17 | 2015-06-02 | Emory University | Virus-like particles, methods of preparation, and immunogenic compositions |
| RU2322257C2 (ru) | 2002-06-20 | 2008-04-20 | Цитос Байотекнолоджи Аг | КОМПОЗИЦИИ, СОДЕРЖАЩИЕ CpG-ОЛИГОНУКЛЕОТИДЫ И ВИРУСОПОДОБНЫЕ ЧАСТИЦЫ, ДЛЯ ПРИМЕНЕНИЯ В КАЧЕСТВЕ АДЪЮВАНТОВ |
| GB0226722D0 (en) | 2002-11-15 | 2002-12-24 | Glaxo Group Ltd | Vaccine |
| US6910730B2 (en) * | 2002-12-25 | 2005-06-28 | Shiroki Corporation | Mounting device for mounting a cable-operated window regulator |
| US8080255B2 (en) * | 2003-07-11 | 2011-12-20 | Novavax Inc. | Functional influenza virus like particles (VLPs) |
| US8592197B2 (en) | 2003-07-11 | 2013-11-26 | Novavax, Inc. | Functional influenza virus-like particles (VLPs) |
| US8574590B2 (en) | 2003-07-30 | 2013-11-05 | Integral Molecular, Inc. | Lipoparticles comprising proteins, methods of making, and using the same |
| US7404963B2 (en) | 2004-10-01 | 2008-07-29 | The University Of South Florida | Flagellin-based adjuvants and vaccines |
| TWI488968B (zh) * | 2005-10-18 | 2015-06-21 | Novavax Inc | 功能性流行性感冒類病毒顆粒（ＶＬＰｓ） |
| US8437251B2 (en) | 2005-12-22 | 2013-05-07 | Qualcomm Incorporated | Methods and apparatus for communicating transmission backlog information |
| CA2657955A1 (en) * | 2006-07-27 | 2008-08-07 | Ligocyte Pharmaceuticals, Inc. | Chimeric virus-like particles |
| CA2615372A1 (en) * | 2007-07-13 | 2009-01-13 | Marc-Andre D'aoust | Influenza virus-like particles (vlps) comprising hemagglutinin |
| NO2324113T3 (enExample) * | 2008-08-08 | 2018-07-28 |
-
2007
- 2007-07-27 JP JP2009521850A patent/JP5683811B2/ja not_active Expired - Fee Related
- 2007-07-27 US US12/375,376 patent/US9439959B2/en not_active Expired - Fee Related
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- 2007-07-27 EP EP07872585A patent/EP2044198A4/en not_active Withdrawn
- 2007-07-27 CA CA2659275A patent/CA2659275C/en not_active Expired - Fee Related
- 2007-07-27 AU AU2007345768A patent/AU2007345768B2/en not_active Ceased
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050186621A1 (en) * | 2000-06-23 | 2005-08-25 | American Cyanamid Company | Assembly of wild-type and chimeric influenza virus-like particles (VLPs) |
| WO2003066868A1 (en) * | 2002-02-04 | 2003-08-14 | Oxford Biomedica (Uk) Limited | Retroviral vectors pseudotyped with influenza virus hemagglutinin for gene delivery |
| WO2004042001A2 (en) * | 2002-05-17 | 2004-05-21 | Emory University | Virus-like particles, methods of preparation, and immonogenic compositions |
Non-Patent Citations (2)
| Title |
|---|
| Bosch, V. et al., J Gen Virol. 2001 vol. 82 pages 2485-2494 * |
| Guo, L. et al., Virology. 2003 vol. 313 pages 502-513 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CA2659275C (en) | 2017-01-10 |
| JP2009544318A (ja) | 2009-12-17 |
| EP2044198A4 (en) | 2010-09-08 |
| AU2007345768A1 (en) | 2008-08-07 |
| WO2008094197A2 (en) | 2008-08-07 |
| WO2008094197A3 (en) | 2008-10-16 |
| EP2044198A2 (en) | 2009-04-08 |
| CA2659275A1 (en) | 2008-08-07 |
| US9439959B2 (en) | 2016-09-13 |
| JP5683811B2 (ja) | 2015-03-11 |
| US20100074915A1 (en) | 2010-03-25 |
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