AU2006320275B2 - Synthesis of error-minimized nucleic acid molecules - Google Patents

Synthesis of error-minimized nucleic acid molecules Download PDF

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Publication number
AU2006320275B2
AU2006320275B2 AU2006320275A AU2006320275A AU2006320275B2 AU 2006320275 B2 AU2006320275 B2 AU 2006320275B2 AU 2006320275 A AU2006320275 A AU 2006320275A AU 2006320275 A AU2006320275 A AU 2006320275A AU 2006320275 B2 AU2006320275 B2 AU 2006320275B2
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molecules
nucleotide sequence
fragments
sequence
intended
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AU2006320275A1 (en
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Lei Young
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Viridos Inc
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Synthetic Genomics Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AU2006320275A 2005-12-02 2006-12-04 Synthesis of error-minimized nucleic acid molecules Active AU2006320275B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US74146905P 2005-12-02 2005-12-02
US60/741,469 2005-12-02
PCT/US2006/046457 WO2007065035A2 (en) 2005-12-02 2006-12-04 Synthesis of error-minimized nucleic acid molecules

Publications (2)

Publication Number Publication Date
AU2006320275A1 AU2006320275A1 (en) 2007-06-07
AU2006320275B2 true AU2006320275B2 (en) 2012-06-07

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ID=38092910

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2006320275A Active AU2006320275B2 (en) 2005-12-02 2006-12-04 Synthesis of error-minimized nucleic acid molecules

Country Status (6)

Country Link
US (1) US7704690B2 (https=)
EP (1) EP1963525B2 (https=)
JP (1) JP4789271B2 (https=)
AU (1) AU2006320275B2 (https=)
CA (1) CA2642514C (https=)
WO (1) WO2007065035A2 (https=)

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Publication number Priority date Publication date Assignee Title
CA2618699C (en) * 2005-08-11 2012-10-02 J. Craig Venter Institute, Inc. In vitro recombination method
WO2008115632A2 (en) * 2007-02-09 2008-09-25 The Regents Of The University Of California Method for recombining dna sequences and compositions related thereto
AU2009214435C1 (en) * 2008-02-15 2014-07-17 Synthetic Genomics, Inc. Methods for in vitro joining and combinatorial assembly of nucleic acid molecules
US10093552B2 (en) 2008-02-22 2018-10-09 James Weifu Lee Photovoltaic panel-interfaced solar-greenhouse distillation systems
US9259662B2 (en) 2008-02-22 2016-02-16 James Weifu Lee Photovoltaic panel-interfaced solar-greenhouse distillation systems
US8986963B2 (en) * 2008-02-23 2015-03-24 James Weifu Lee Designer calvin-cycle-channeled production of butanol and related higher alcohols
PL2250276T3 (pl) 2008-02-23 2017-07-31 James Weifu Lee Organizmy zaprojektowane do fotobiologicznego wytwarzania butanolu z ditlenku węgla i wody
US9080211B2 (en) * 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
JP5794572B2 (ja) * 2008-10-24 2015-10-14 エピセンター テクノロジーズ コーポレイションＥｐｉｃｅｎｔｒｅ Ｔｅｃｈｎｏｌｏｇｉｅｓ Ｃｏｒｐｏｒａｔｉｏｎ 核酸を修飾するためのトランスポゾン末端組成物及び方法
US10174368B2 (en) 2009-09-10 2019-01-08 Centrillion Technology Holdings Corporation Methods and systems for sequencing long nucleic acids
US10072287B2 (en) 2009-09-10 2018-09-11 Centrillion Technology Holdings Corporation Methods of targeted sequencing
US20120252682A1 (en) * 2011-04-01 2012-10-04 Maples Corporate Services Limited Methods and systems for sequencing nucleic acids
US12595500B2 (en) 2011-09-26 2026-04-07 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
CN103945931B (zh) 2011-09-26 2017-03-22 基因技术股份公司 高效的小体积核酸合成
WO2014153188A2 (en) 2013-03-14 2014-09-25 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
WO2014106167A1 (en) * 2012-12-31 2014-07-03 Advanced Liquid Logic, Inc. Digital microfluidic gene synthesis and error correction
WO2016011174A1 (en) 2014-07-15 2016-01-21 Life Technologies Corporation Compositions and methods for nucleic acid assembly
US10407676B2 (en) 2014-12-09 2019-09-10 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
US20180195099A1 (en) 2015-07-07 2018-07-12 Thermo Fisher Scientific Geneart Gmbh Enzymatic synthesis of nucleic acid sequences
EP3763818A1 (en) 2015-10-06 2021-01-13 Pierce Biotechnology, Inc. Devices and methods for producing proteins
GB2566986A (en) 2017-09-29 2019-04-03 Evonetix Ltd Error detection during hybridisation of target double-stranded nucleic acid
EP3768858A1 (en) * 2018-03-19 2021-01-27 Modernatx, Inc. Assembly and error reduction of synthetic genes from oligonucleotides
US20210147830A1 (en) 2018-06-29 2021-05-20 Thermo Fisher Scientific Geneart Gmbh High throughput assembly of nucleic acid molecules
GB201905303D0 (en) 2019-04-15 2019-05-29 Thermo Fisher Scient Geneart Gmbh Multiplex assembly of nucleic acid molecules
US20240025939A1 (en) 2020-03-06 2024-01-25 Life Technologies Corporation High sequence fidelity nucleic acid synthesis and assembly
WO2024132094A1 (en) 2022-12-19 2024-06-27 Thermo Fisher Scientific Geneart Gmbh Retrieval of sequence-verified nucleic acid molecules

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050255477A1 (en) * 2002-12-10 2005-11-17 Carr Peter A Methods for high fidelity production of long nucleic acid molecules

Family Cites Families (8)

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Publication number Priority date Publication date Assignee Title
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US20040023327A1 (en) * 1995-12-07 2004-02-05 Short Jay M. End selection in directed evolution
ES2546450T3 (es) * 2000-04-07 2015-09-23 Eiken Kagaku Kabushiki Kaisha Método para amplificar y detectar un ácido nucleico usando ácido nucleico bicatenario como molde
DE60137582D1 (de) * 2000-12-01 2009-03-19 Cornell Res Foundation Inc Nachweis von nukleinsäureunterschieden mit einer kombination aus endonukleasespaltungs- und ligationsreaktionen
US20050106590A1 (en) * 2003-05-22 2005-05-19 Lathrop Richard H. Method for producing a synthetic gene or other DNA sequence
EP1781786B1 (en) * 2004-08-27 2011-01-26 Wisconsin Alumni Research Foundation Method of error reduction in nucleic acid populations
US20070196834A1 (en) * 2005-09-09 2007-08-23 Francesco Cerrina Method and system for the generation of large double stranded DNA fragments

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050255477A1 (en) * 2002-12-10 2005-11-17 Carr Peter A Methods for high fidelity production of long nucleic acid molecules

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
YOUNG, L. et al., Nucleic Acids Research, 2004, vol. 32, e59 pages 1-6 *

Also Published As

Publication number Publication date
EP1963525B1 (en) 2013-11-20
WO2007065035A3 (en) 2007-11-22
JP2009518013A (ja) 2009-05-07
US20070128649A1 (en) 2007-06-07
EP1963525B2 (en) 2017-10-11
WO2007065035A2 (en) 2007-06-07
US7704690B2 (en) 2010-04-27
EP1963525A2 (en) 2008-09-03
AU2006320275A1 (en) 2007-06-07
JP4789271B2 (ja) 2011-10-12
CA2642514A1 (en) 2007-06-07
CA2642514C (en) 2011-06-07
EP1963525A4 (en) 2009-03-18

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