JP2009518013A5 - - Google Patents
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- Publication number
- JP2009518013A5 JP2009518013A5 JP2008543550A JP2008543550A JP2009518013A5 JP 2009518013 A5 JP2009518013 A5 JP 2009518013A5 JP 2008543550 A JP2008543550 A JP 2008543550A JP 2008543550 A JP2008543550 A JP 2008543550A JP 2009518013 A5 JP2009518013 A5 JP 2009518013A5
- Authority
- JP
- Japan
- Prior art keywords
- molecules
- fragments
- endonuclease
- oligonucleotide
- fragment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims description 66
- 239000012634 fragment Substances 0.000 claims description 55
- 108091034117 Oligonucleotide Proteins 0.000 claims description 36
- 239000002773 nucleotide Substances 0.000 claims description 28
- 125000003729 nucleotide group Chemical group 0.000 claims description 28
- 102000004533 Endonucleases Human genes 0.000 claims description 20
- 108010042407 Endonucleases Proteins 0.000 claims description 20
- 230000002194 synthesizing effect Effects 0.000 claims description 12
- 238000003752 polymerase chain reaction Methods 0.000 claims description 7
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 6
- 108090000623 proteins and genes Proteins 0.000 claims description 6
- 230000015572 biosynthetic process Effects 0.000 claims description 5
- 108020004707 nucleic acids Proteins 0.000 claims description 5
- 102000039446 nucleic acids Human genes 0.000 claims description 5
- 150000007523 nucleic acids Chemical class 0.000 claims description 5
- 238000003786 synthesis reaction Methods 0.000 claims description 5
- 102000007260 Deoxyribonuclease I Human genes 0.000 claims description 4
- 108010008532 Deoxyribonuclease I Proteins 0.000 claims description 4
- 238000000137 annealing Methods 0.000 claims description 4
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 238000010367 cloning Methods 0.000 claims description 4
- 238000012163 sequencing technique Methods 0.000 claims description 4
- 239000013598 vector Substances 0.000 claims description 4
- 238000012545 processing Methods 0.000 claims description 3
- 102000004099 Deoxyribonuclease (Pyrimidine Dimer) Human genes 0.000 claims description 2
- 108010082610 Deoxyribonuclease (Pyrimidine Dimer) Proteins 0.000 claims description 2
- 241000588724 Escherichia coli Species 0.000 claims description 2
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 claims description 2
- 230000003321 amplification Effects 0.000 claims description 2
- 229910052748 manganese Inorganic materials 0.000 claims description 2
- 239000011572 manganese Substances 0.000 claims description 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 4
- 108020004414 DNA Proteins 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 238000007702 DNA assembly Methods 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 108060002716 Exonuclease Proteins 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 102000023732 binding proteins Human genes 0.000 description 1
- 108091008324 binding proteins Proteins 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 102000013165 exonuclease Human genes 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US74146905P | 2005-12-02 | 2005-12-02 | |
| US60/741,469 | 2005-12-02 | ||
| PCT/US2006/046457 WO2007065035A2 (en) | 2005-12-02 | 2006-12-04 | Synthesis of error-minimized nucleic acid molecules |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2009518013A JP2009518013A (ja) | 2009-05-07 |
| JP2009518013A5 true JP2009518013A5 (https=) | 2011-04-07 |
| JP4789271B2 JP4789271B2 (ja) | 2011-10-12 |
Family
ID=38092910
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2008543550A Active JP4789271B2 (ja) | 2005-12-02 | 2006-12-04 | エラーが最小化された核酸分子の合成 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US7704690B2 (https=) |
| EP (1) | EP1963525B2 (https=) |
| JP (1) | JP4789271B2 (https=) |
| AU (1) | AU2006320275B2 (https=) |
| CA (1) | CA2642514C (https=) |
| WO (1) | WO2007065035A2 (https=) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2618699C (en) * | 2005-08-11 | 2012-10-02 | J. Craig Venter Institute, Inc. | In vitro recombination method |
| WO2008115632A2 (en) * | 2007-02-09 | 2008-09-25 | The Regents Of The University Of California | Method for recombining dna sequences and compositions related thereto |
| AU2009214435C1 (en) * | 2008-02-15 | 2014-07-17 | Synthetic Genomics, Inc. | Methods for in vitro joining and combinatorial assembly of nucleic acid molecules |
| US10093552B2 (en) | 2008-02-22 | 2018-10-09 | James Weifu Lee | Photovoltaic panel-interfaced solar-greenhouse distillation systems |
| US9259662B2 (en) | 2008-02-22 | 2016-02-16 | James Weifu Lee | Photovoltaic panel-interfaced solar-greenhouse distillation systems |
| US8986963B2 (en) * | 2008-02-23 | 2015-03-24 | James Weifu Lee | Designer calvin-cycle-channeled production of butanol and related higher alcohols |
| PL2250276T3 (pl) | 2008-02-23 | 2017-07-31 | James Weifu Lee | Organizmy zaprojektowane do fotobiologicznego wytwarzania butanolu z ditlenku węgla i wody |
| US9080211B2 (en) * | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| JP5794572B2 (ja) * | 2008-10-24 | 2015-10-14 | エピセンター テクノロジーズ コーポレイションＥｐｉｃｅｎｔｒｅ Ｔｅｃｈｎｏｌｏｇｉｅｓ Ｃｏｒｐｏｒａｔｉｏｎ | 核酸を修飾するためのトランスポゾン末端組成物及び方法 |
| US10174368B2 (en) | 2009-09-10 | 2019-01-08 | Centrillion Technology Holdings Corporation | Methods and systems for sequencing long nucleic acids |
| US10072287B2 (en) | 2009-09-10 | 2018-09-11 | Centrillion Technology Holdings Corporation | Methods of targeted sequencing |
| US20120252682A1 (en) * | 2011-04-01 | 2012-10-04 | Maples Corporate Services Limited | Methods and systems for sequencing nucleic acids |
| US12595500B2 (en) | 2011-09-26 | 2026-04-07 | Life Technologies Corporation | High efficiency, small volume nucleic acid synthesis |
| CN103945931B (zh) | 2011-09-26 | 2017-03-22 | 基因技术股份公司 | 高效的小体积核酸合成 |
| WO2014153188A2 (en) | 2013-03-14 | 2014-09-25 | Life Technologies Corporation | High efficiency, small volume nucleic acid synthesis |
| WO2014106167A1 (en) * | 2012-12-31 | 2014-07-03 | Advanced Liquid Logic, Inc. | Digital microfluidic gene synthesis and error correction |
| WO2016011174A1 (en) | 2014-07-15 | 2016-01-21 | Life Technologies Corporation | Compositions and methods for nucleic acid assembly |
| US10407676B2 (en) | 2014-12-09 | 2019-09-10 | Life Technologies Corporation | High efficiency, small volume nucleic acid synthesis |
| US20180195099A1 (en) | 2015-07-07 | 2018-07-12 | Thermo Fisher Scientific Geneart Gmbh | Enzymatic synthesis of nucleic acid sequences |
| EP3763818A1 (en) | 2015-10-06 | 2021-01-13 | Pierce Biotechnology, Inc. | Devices and methods for producing proteins |
| GB2566986A (en) | 2017-09-29 | 2019-04-03 | Evonetix Ltd | Error detection during hybridisation of target double-stranded nucleic acid |
| EP3768858A1 (en) * | 2018-03-19 | 2021-01-27 | Modernatx, Inc. | Assembly and error reduction of synthetic genes from oligonucleotides |
| US20210147830A1 (en) | 2018-06-29 | 2021-05-20 | Thermo Fisher Scientific Geneart Gmbh | High throughput assembly of nucleic acid molecules |
| GB201905303D0 (en) | 2019-04-15 | 2019-05-29 | Thermo Fisher Scient Geneart Gmbh | Multiplex assembly of nucleic acid molecules |
| US20240025939A1 (en) | 2020-03-06 | 2024-01-25 | Life Technologies Corporation | High sequence fidelity nucleic acid synthesis and assembly |
| WO2024132094A1 (en) | 2022-12-19 | 2024-06-27 | Thermo Fisher Scientific Geneart Gmbh | Retrieval of sequence-verified nucleic acid molecules |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5605793A (en) * | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US6165793A (en) * | 1996-03-25 | 2000-12-26 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US20040023327A1 (en) * | 1995-12-07 | 2004-02-05 | Short Jay M. | End selection in directed evolution |
| ES2546450T3 (es) * | 2000-04-07 | 2015-09-23 | Eiken Kagaku Kabushiki Kaisha | Método para amplificar y detectar un ácido nucleico usando ácido nucleico bicatenario como molde |
| DE60137582D1 (de) * | 2000-12-01 | 2009-03-19 | Cornell Res Foundation Inc | Nachweis von nukleinsäureunterschieden mit einer kombination aus endonukleasespaltungs- und ligationsreaktionen |
| US7879580B2 (en) * | 2002-12-10 | 2011-02-01 | Massachusetts Institute Of Technology | Methods for high fidelity production of long nucleic acid molecules |
| US20050106590A1 (en) * | 2003-05-22 | 2005-05-19 | Lathrop Richard H. | Method for producing a synthetic gene or other DNA sequence |
| EP1781786B1 (en) * | 2004-08-27 | 2011-01-26 | Wisconsin Alumni Research Foundation | Method of error reduction in nucleic acid populations |
| US20070196834A1 (en) * | 2005-09-09 | 2007-08-23 | Francesco Cerrina | Method and system for the generation of large double stranded DNA fragments |
-
2006
- 2006-12-04 JP JP2008543550A patent/JP4789271B2/ja active Active
- 2006-12-04 WO PCT/US2006/046457 patent/WO2007065035A2/en not_active Ceased
- 2006-12-04 EP EP06844856.2A patent/EP1963525B2/en active Active
- 2006-12-04 AU AU2006320275A patent/AU2006320275B2/en active Active
- 2006-12-04 CA CA2642514A patent/CA2642514C/en active Active
- 2006-12-04 US US11/633,686 patent/US7704690B2/en active Active
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