AU2005317279C1 - Purification of immunoglobulins - Google Patents

Purification of immunoglobulins Download PDF

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Publication number
AU2005317279C1
AU2005317279C1 AU2005317279A AU2005317279A AU2005317279C1 AU 2005317279 C1 AU2005317279 C1 AU 2005317279C1 AU 2005317279 A AU2005317279 A AU 2005317279A AU 2005317279 A AU2005317279 A AU 2005317279A AU 2005317279 C1 AU2005317279 C1 AU 2005317279C1
Authority
AU
Australia
Prior art keywords
separation matrix
antibodies
protein
antibody
column
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
AU2005317279A
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English (en)
Other versions
AU2005317279A1 (en
AU2005317279B2 (en
Inventor
Per-Mikael Aberg
Hans Berg
Hans J. Johansson
Gunnar Malmquist
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Bioprocess R&D AB
Original Assignee
Cytiva Bioprocess R&D AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=36588164&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AU2005317279(C1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from SE0403057A external-priority patent/SE0403057D0/sv
Application filed by Cytiva Bioprocess R&D AB filed Critical Cytiva Bioprocess R&D AB
Publication of AU2005317279A1 publication Critical patent/AU2005317279A1/en
Publication of AU2005317279B2 publication Critical patent/AU2005317279B2/en
Application granted granted Critical
Publication of AU2005317279C1 publication Critical patent/AU2005317279C1/en
Assigned to GE HEALTHCARE BIOPROCESS R&D AB reassignment GE HEALTHCARE BIOPROCESS R&D AB Request for Assignment Assignors: GE HEALTHCARE BIO-SCIENCES AB
Assigned to CYTIVA BIOPROCESS R&D AB reassignment CYTIVA BIOPROCESS R&D AB Request to Amend Deed and Register Assignors: GE HEALTHCARE BIOPROCESS R&D AB
Anticipated expiration legal-status Critical
Expired legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B01J20/28002—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their physical properties
    • B01J20/28004—Sorbent size or size distribution, e.g. particle size
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B01D15/1871—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in series
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/461—Flow patterns using more than one column with serial coupling of separation columns

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Fodder In General (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AU2005317279A 2004-12-14 2005-12-12 Purification of immunoglobulins Expired AU2005317279C1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
SE0403057A SE0403057D0 (sv) 2004-12-14 2004-12-14 Purification of immunoglobulins
SE0403057-3 2004-12-14
US63831604P 2004-12-22 2004-12-22
US60/638,316 2004-12-22
PCT/SE2005/001900 WO2006065208A1 (en) 2004-12-14 2005-12-12 Purification of immunoglobulins

Publications (3)

Publication Number Publication Date
AU2005317279A1 AU2005317279A1 (en) 2006-06-22
AU2005317279B2 AU2005317279B2 (en) 2011-02-24
AU2005317279C1 true AU2005317279C1 (en) 2014-07-17

Family

ID=36588164

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2005317279A Expired AU2005317279C1 (en) 2004-12-14 2005-12-12 Purification of immunoglobulins

Country Status (5)

Country Link
EP (1) EP1830947A4 (https=)
JP (1) JP5787461B2 (https=)
AU (1) AU2005317279C1 (https=)
CA (1) CA2586803C (https=)
WO (1) WO2006065208A1 (https=)

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EP3050963B1 (en) 2005-03-31 2019-09-18 Chugai Seiyaku Kabushiki Kaisha Process for production of polypeptide by regulation of assembly
ES2892925T3 (es) 2006-03-31 2022-02-07 Chugai Pharmaceutical Co Ltd Métodos para controlar la farmacocinética en sangre de anticuerpos
US9670269B2 (en) 2006-03-31 2017-06-06 Chugai Seiyaku Kabushiki Kaisha Methods of modifying antibodies for purification of bispecific antibodies
EP2066419B1 (en) 2006-09-29 2015-11-11 GE Healthcare Bio-Sciences AB Chromatography ligand comprising domain c from staphyloccocus aureus protein a for antibody isolation
SG149759A1 (en) * 2007-07-10 2009-02-27 Millipore Corp Media for affinity chromatography
CN101874042B9 (zh) 2007-09-26 2019-01-01 中外制药株式会社 利用cdr的氨基酸取代来改变抗体等电点的方法
AU2008304748C1 (en) 2007-09-26 2014-06-12 Chugai Seiyaku Kabushiki Kaisha Modified antibody constant region
US8277649B2 (en) * 2009-12-14 2012-10-02 General Electric Company Membranes and associated methods for purification of antibodies
JP5889181B2 (ja) 2010-03-04 2016-03-22 中外製薬株式会社 抗体定常領域改変体
JP5917417B2 (ja) * 2010-03-05 2016-05-11 ベーリンガー インゲルハイム インターナショナル ゲゼルシャフト ミット ベシュレンクテル ハフツング 抗体の選択的強化
US8859294B2 (en) 2010-03-17 2014-10-14 Panasonic Corporation Method for binding a protein consisting of protein A or consisting of at least one domain of the A to E domains of the protein A to the substrate
WO2011114405A1 (en) * 2010-03-17 2011-09-22 Panasonic Corporation A method for binding a protein consisting of protein a or consisting of at least one domain of the a to e domains of the protein a to the substrate
JP5998050B2 (ja) 2010-03-31 2016-09-28 Jsr株式会社 アフィニティークロマトグラフィー用充填剤
WO2011125673A1 (ja) 2010-03-31 2011-10-13 Jsr株式会社 アフィニティークロマトグラフィー用充填剤
PH12016502073B1 (en) 2010-11-17 2024-04-05 Chugai Pharmaceutical Co Ltd Multi-specific antigen-binding molecule having alternative function to function of blood coagulation factor viii
RS67768B1 (sr) 2010-11-30 2026-03-31 Chugai Pharmaceutical Co Ltd Terapijski agens koji indukuje citotoksičnost
WO2015030094A1 (ja) 2013-08-30 2015-03-05 株式会社カネカ Ｆａｂ領域結合性ペプチド
EP3042192A4 (en) * 2013-09-04 2017-04-26 Sten Ohlson Weak affinity chromatography
JP6230853B2 (ja) * 2013-09-06 2017-11-15 株式会社日立製作所 タンパク質の精製方法及び精製装置
JP6534615B2 (ja) 2013-09-27 2019-06-26 中外製薬株式会社 ポリペプチド異種多量体の製造方法
US20170043320A1 (en) 2013-11-27 2017-02-16 Jsr Corporation Solid-phase carrier, production method for solid-phase carrier, carrier for affinity refining, production method for filler for affinity chromatography, filler for affinity chromatography, chromatography column, and refining method
US10610807B2 (en) 2014-01-17 2020-04-07 Repligen Corporation Sterilizing chromatography columns
CN106795215A (zh) 2014-06-24 2017-05-31 通用电气医疗集团生物工艺研发股份公司 层析方法
EP3162809B1 (en) 2014-06-27 2021-08-04 JSR Corporation Carrier for affinity chromatography
WO2016031902A1 (ja) * 2014-08-28 2016-03-03 株式会社カネカ Ｆａｂ領域含有ペプチド用アフィニティー分離マトリックス
WO2016121701A1 (ja) 2015-01-26 2016-08-04 株式会社カネカ 免疫グロブリンκ鎖可変領域含有タンパク質精製用アフィニティー分離マトリックス
JP6663360B2 (ja) 2015-01-26 2020-03-11 株式会社カネカ 変異型免疫グロブリンκ鎖可変領域結合性ペプチド
JP7082484B2 (ja) 2015-04-01 2022-06-08 中外製薬株式会社 ポリペプチド異種多量体の製造方法
CN108368166B (zh) * 2015-12-28 2023-03-28 中外制药株式会社 提高含fc区多肽纯化效率的方法
MA44780A (fr) 2016-04-28 2019-03-06 Chugai Pharmaceutical Co Ltd Préparation contenant un anticorps
WO2017195638A1 (ja) 2016-05-09 2017-11-16 株式会社カネカ 抗体またはκ鎖可変領域含有抗体断片の精製方法
WO2017195641A1 (ja) * 2016-05-11 2017-11-16 株式会社カネカ アフィニティ分離マトリックスの製造方法およびアフィニティ分離マトリックス
GB201703116D0 (en) * 2017-02-27 2017-04-12 Ge Healthcare Bioprocess R&D Ab A seperation matrix and a method of seperating antibodies
CN110461358A (zh) 2017-03-31 2019-11-15 公立大学法人奈良县立医科大学 可用于预防和/或治疗凝血因子ⅸ异常、包含代替凝血因子ⅷ的功能的多特异性抗原结合分子的药物组合物
AU2018361430B2 (en) 2017-11-01 2025-08-14 Chugai Seiyaku Kabushiki Kaisha Antibody variant and isoform with lowered biological activity

Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4444879A (en) * 1981-01-29 1984-04-24 Science Research Center, Inc. Immunoassay with article having support film and immunological counterpart of analyte
US5089605A (en) * 1987-03-13 1992-02-18 Repligen Corporation Immobilized immunoglobulin-binding proteins
US5672276A (en) * 1992-06-19 1997-09-30 Biosepra Inc. Passivated porous polymer supports and methods for the preparation and use of same
US5907016A (en) * 1993-03-29 1999-05-25 Virginia Tech Intellectual Properties,Inc. Method of coupling ligands onto supports and products produced therefrom
EP0955332A1 (en) * 1997-01-07 1999-11-10 Kaneka Corporation Cellulosic particles, spherical object comprising cross-linked polymer particles, and adsorbent for body fluid purification
WO2002053254A1 (en) * 2000-12-29 2002-07-11 Amersham Biosciences Ab A method for producing liquid chromatography matrices
US6428707B1 (en) * 1997-02-04 2002-08-06 Amersham Pharmacia Biotech Ab Adsorption/separation method and a medium for adsorption/separation
US6602990B1 (en) * 1996-04-11 2003-08-05 Amersham Biosciences Ab Process for the production of a porous cross-linked polysaccharide gel and its use as a gel filtration media and in chromatography
WO2003080655A1 (en) * 2002-03-25 2003-10-02 Amersham Biosciences Ab A mutated immunoglobulin-binding protein
WO2004074211A1 (en) * 2003-02-21 2004-09-02 Amersham Biosciences Ab Inorganic beads with hierarchical pore structures
EP1500431A2 (en) * 2003-07-22 2005-01-26 Millipore (U.K.) Ltd. Immobilised affinity medium and its use in separation
WO2006024497A1 (en) * 2004-08-30 2006-03-09 Lonza Biologics Plc. Affinity- plus ion exchange- chromatography for purifying antibodies

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SE9503925D0 (sv) * 1995-11-07 1995-11-07 Pharmacia Biotech Ab Separationsmedium för IgG

Patent Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4444879A (en) * 1981-01-29 1984-04-24 Science Research Center, Inc. Immunoassay with article having support film and immunological counterpart of analyte
US5089605A (en) * 1987-03-13 1992-02-18 Repligen Corporation Immobilized immunoglobulin-binding proteins
US5672276A (en) * 1992-06-19 1997-09-30 Biosepra Inc. Passivated porous polymer supports and methods for the preparation and use of same
US5907016A (en) * 1993-03-29 1999-05-25 Virginia Tech Intellectual Properties,Inc. Method of coupling ligands onto supports and products produced therefrom
US6602990B1 (en) * 1996-04-11 2003-08-05 Amersham Biosciences Ab Process for the production of a porous cross-linked polysaccharide gel and its use as a gel filtration media and in chromatography
EP0955332A1 (en) * 1997-01-07 1999-11-10 Kaneka Corporation Cellulosic particles, spherical object comprising cross-linked polymer particles, and adsorbent for body fluid purification
US6428707B1 (en) * 1997-02-04 2002-08-06 Amersham Pharmacia Biotech Ab Adsorption/separation method and a medium for adsorption/separation
WO2002053254A1 (en) * 2000-12-29 2002-07-11 Amersham Biosciences Ab A method for producing liquid chromatography matrices
WO2003080655A1 (en) * 2002-03-25 2003-10-02 Amersham Biosciences Ab A mutated immunoglobulin-binding protein
WO2004074211A1 (en) * 2003-02-21 2004-09-02 Amersham Biosciences Ab Inorganic beads with hierarchical pore structures
EP1500431A2 (en) * 2003-07-22 2005-01-26 Millipore (U.K.) Ltd. Immobilised affinity medium and its use in separation
WO2006024497A1 (en) * 2004-08-30 2006-03-09 Lonza Biologics Plc. Affinity- plus ion exchange- chromatography for purifying antibodies

Also Published As

Publication number Publication date
WO2006065208A1 (en) 2006-06-22
AU2005317279A1 (en) 2006-06-22
EP1830947A4 (en) 2012-04-18
AU2005317279B2 (en) 2011-02-24
JP5787461B2 (ja) 2015-09-30
CA2586803C (en) 2012-12-11
JP2008523140A (ja) 2008-07-03
CA2586803A1 (en) 2006-06-22
EP1830947A1 (en) 2007-09-12

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Legal Events

Date Code Title Description
CB Opposition filed

Opponent name: STEPHEN DEAN SHARP AND DAVID JOHN HUGHES

ON Decision of a delegate or deputy of the commissioner of patents (result of patent office hearing)

Free format text: APO 10: LACK OF CLARITY NOT ESTABLISHED - FAIR BASIS, CLAIMS NEED NOT BE LIMITED TO THE PREFERRED EMBODIMENTS - LACK OF FAIR BASIS NOT ESTABLISHED.

Opponent name: STEPHEN DEAN SHARP DAVID JOHN HUGHES

DA2 Applications for amendment section 104

Free format text: THE NATURE OF THE AMENDMENT IS AS SHOWN IN THE STATEMENT(S) FILED 13 MAR 2014 .

DA3 Amendments made section 104

Free format text: THE NATURE OF THE AMENDMENT IS AS SHOWN IN THE STATEMENT(S) FILED 13 MAR 2014

FGA Letters patent sealed or granted (standard patent)
PC Assignment registered

Owner name: GE HEALTHCARE BIOPROCESS R&D AB

Free format text: FORMER OWNER WAS: GE HEALTHCARE BIO-SCIENCES AB

HB Alteration of name in register

Owner name: CYTIVA BIOPROCESS R&D AB

Free format text: FORMER NAME(S): GE HEALTHCARE BIOPROCESS R&D AB

MK14 Patent ceased section 143(a) (annual fees not paid) or expired