AU2004311384B2 - Cell cycle genes and related methods of using - Google Patents
Cell cycle genes and related methods of using Download PDFInfo
- Publication number
- AU2004311384B2 AU2004311384B2 AU2004311384A AU2004311384A AU2004311384B2 AU 2004311384 B2 AU2004311384 B2 AU 2004311384B2 AU 2004311384 A AU2004311384 A AU 2004311384A AU 2004311384 A AU2004311384 A AU 2004311384A AU 2004311384 B2 AU2004311384 B2 AU 2004311384B2
- Authority
- AU
- Australia
- Prior art keywords
- plant
- wood
- seq
- genes
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 238000000034 method Methods 0.000 title description 165
- 108700021031 cdc Genes Proteins 0.000 title description 81
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 641
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 174
- 102000040430 polynucleotide Human genes 0.000 claims abstract description 71
- 108091033319 polynucleotide Proteins 0.000 claims abstract description 71
- 239000002157 polynucleotide Substances 0.000 claims abstract description 71
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 61
- 230000001105 regulatory effect Effects 0.000 claims abstract description 39
- 230000015572 biosynthetic process Effects 0.000 claims abstract description 32
- 102000004169 proteins and genes Human genes 0.000 claims description 424
- 241000196324 Embryophyta Species 0.000 claims description 304
- 239000002023 wood Substances 0.000 claims description 168
- 210000004027 cell Anatomy 0.000 claims description 112
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 94
- 150000007523 nucleic acids Chemical group 0.000 claims description 88
- 108020004414 DNA Proteins 0.000 claims description 78
- 230000001965 increasing effect Effects 0.000 claims description 68
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 35
- 230000012010 growth Effects 0.000 claims description 32
- 230000009261 transgenic effect Effects 0.000 claims description 30
- 230000018109 developmental process Effects 0.000 claims description 29
- 244000166124 Eucalyptus globulus Species 0.000 claims description 28
- 238000011161 development Methods 0.000 claims description 28
- 230000001976 improved effect Effects 0.000 claims description 25
- 239000000203 mixture Substances 0.000 claims description 23
- 229920005610 lignin Polymers 0.000 claims description 22
- 230000032823 cell division Effects 0.000 claims description 21
- 239000000835 fiber Substances 0.000 claims description 21
- 241000218602 Pinus <genus> Species 0.000 claims description 20
- 241000894007 species Species 0.000 claims description 20
- 235000005205 Pinus Nutrition 0.000 claims description 19
- 230000000295 complement effect Effects 0.000 claims description 18
- 239000001913 cellulose Substances 0.000 claims description 17
- 229920002678 cellulose Polymers 0.000 claims description 17
- 238000004519 manufacturing process Methods 0.000 claims description 17
- 230000002829 reductive effect Effects 0.000 claims description 17
- 238000006243 chemical reaction Methods 0.000 claims description 16
- 230000001850 reproductive effect Effects 0.000 claims description 15
- 230000003247 decreasing effect Effects 0.000 claims description 14
- 210000002421 cell wall Anatomy 0.000 claims description 13
- 230000000692 anti-sense effect Effects 0.000 claims description 12
- 210000001724 microfibril Anatomy 0.000 claims description 11
- 241000238631 Hexapoda Species 0.000 claims description 8
- 208000031888 Mycoses Diseases 0.000 claims description 6
- 230000011712 cell development Effects 0.000 claims description 6
- 238000003860 storage Methods 0.000 claims description 6
- 230000007547 defect Effects 0.000 claims description 5
- 230000001939 inductive effect Effects 0.000 claims description 5
- 238000006116 polymerization reaction Methods 0.000 claims description 5
- 230000008511 vegetative development Effects 0.000 claims description 5
- 208000005156 Dehydration Diseases 0.000 claims description 4
- 229910019142 PO4 Inorganic materials 0.000 claims description 4
- 229920002472 Starch Polymers 0.000 claims description 4
- 241000607479 Yersinia pestis Species 0.000 claims description 4
- 230000003111 delayed effect Effects 0.000 claims description 4
- 201000010099 disease Diseases 0.000 claims description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 4
- 230000024346 drought recovery Effects 0.000 claims description 4
- 230000035784 germination Effects 0.000 claims description 4
- 230000036541 health Effects 0.000 claims description 4
- 229910001385 heavy metal Inorganic materials 0.000 claims description 4
- 230000002363 herbicidal effect Effects 0.000 claims description 4
- 239000004009 herbicide Substances 0.000 claims description 4
- 230000015784 hyperosmotic salinity response Effects 0.000 claims description 4
- 239000011785 micronutrient Substances 0.000 claims description 4
- 235000013369 micronutrients Nutrition 0.000 claims description 4
- 235000016709 nutrition Nutrition 0.000 claims description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 4
- 239000010452 phosphate Substances 0.000 claims description 4
- 229920005989 resin Polymers 0.000 claims description 4
- 239000011347 resin Substances 0.000 claims description 4
- 235000019698 starch Nutrition 0.000 claims description 4
- 239000008107 starch Substances 0.000 claims description 4
- 230000022131 cell cycle Effects 0.000 abstract description 59
- 229920001184 polypeptide Polymers 0.000 abstract description 57
- 238000010195 expression analysis Methods 0.000 abstract description 20
- 150000004676 glycans Chemical class 0.000 abstract description 8
- 229920001282 polysaccharide Polymers 0.000 abstract description 8
- 239000005017 polysaccharide Substances 0.000 abstract description 8
- 238000003786 synthesis reaction Methods 0.000 abstract description 8
- 235000018102 proteins Nutrition 0.000 description 408
- 125000003275 alpha amino acid group Chemical group 0.000 description 257
- 230000014509 gene expression Effects 0.000 description 185
- 210000001519 tissue Anatomy 0.000 description 139
- 102000004195 Isomerases Human genes 0.000 description 134
- 108090000769 Isomerases Proteins 0.000 description 134
- 108050006400 Cyclin Proteins 0.000 description 126
- 102000016736 Cyclin Human genes 0.000 description 125
- 108091006027 G proteins Proteins 0.000 description 113
- 102000030782 GTP binding Human genes 0.000 description 113
- 108091000058 GTP-Binding Proteins 0.000 description 113
- 239000000523 sample Substances 0.000 description 112
- 108010033040 Histones Proteins 0.000 description 86
- 108090000266 Cyclin-dependent kinases Proteins 0.000 description 81
- 102000003903 Cyclin-dependent kinases Human genes 0.000 description 81
- 108091034117 Oligonucleotide Proteins 0.000 description 64
- 125000001500 prolyl group Chemical group [H]N1C([H])(C(=O)[*])C([H])([H])C([H])([H])C1([H])[H] 0.000 description 59
- 239000002299 complementary DNA Substances 0.000 description 58
- PEEAINPHPNDNGE-JQWIXIFHSA-N Trp-Asp Chemical compound C1=CC=C2C(C[C@H](N)C(=O)N[C@@H](CC(O)=O)C(O)=O)=CNC2=C1 PEEAINPHPNDNGE-JQWIXIFHSA-N 0.000 description 54
- 102000001253 Protein Kinase Human genes 0.000 description 53
- 102000039446 nucleic acids Human genes 0.000 description 53
- 108020004707 nucleic acids Proteins 0.000 description 53
- 108060006633 protein kinase Proteins 0.000 description 53
- 238000009396 hybridization Methods 0.000 description 51
- 239000000047 product Substances 0.000 description 48
- 230000000694 effects Effects 0.000 description 47
- 238000002493 microarray Methods 0.000 description 46
- 125000001151 peptidyl group Chemical group 0.000 description 43
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 37
- 102000003813 Cis-trans-isomerases Human genes 0.000 description 36
- 108090000175 Cis-trans-isomerases Proteins 0.000 description 36
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 36
- 239000002751 oligonucleotide probe Substances 0.000 description 36
- 230000027455 binding Effects 0.000 description 34
- 230000001419 dependent effect Effects 0.000 description 34
- 102100030013 Endoribonuclease Human genes 0.000 description 29
- 101710199605 Endoribonuclease Proteins 0.000 description 29
- 101710113029 Serine/threonine-protein kinase Proteins 0.000 description 29
- 241000018646 Pinus brutia Species 0.000 description 28
- 108091000080 Phosphotransferase Proteins 0.000 description 27
- 241000218621 Pinus radiata Species 0.000 description 27
- 230000000366 juvenile effect Effects 0.000 description 27
- 238000003752 polymerase chain reaction Methods 0.000 description 27
- 238000004458 analytical method Methods 0.000 description 26
- 102000020233 phosphotransferase Human genes 0.000 description 26
- 108090000259 Cyclin D Proteins 0.000 description 25
- 239000002773 nucleotide Substances 0.000 description 25
- 125000003729 nucleotide group Chemical group 0.000 description 25
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 24
- 108020004999 messenger RNA Proteins 0.000 description 24
- 102000003910 Cyclin D Human genes 0.000 description 23
- 239000013615 primer Substances 0.000 description 23
- 230000006870 function Effects 0.000 description 22
- 230000009466 transformation Effects 0.000 description 22
- 235000011613 Pinus brutia Nutrition 0.000 description 21
- 102000012515 Protein kinase domains Human genes 0.000 description 21
- 108050002122 Protein kinase domains Proteins 0.000 description 21
- 239000013598 vector Substances 0.000 description 21
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 20
- 230000003321 amplification Effects 0.000 description 20
- 238000003199 nucleic acid amplification method Methods 0.000 description 20
- 210000004899 c-terminal region Anatomy 0.000 description 19
- 108010031896 Cell Cycle Proteins Proteins 0.000 description 18
- 102000002427 Cyclin B Human genes 0.000 description 18
- 108010068150 Cyclin B Proteins 0.000 description 18
- 230000002792 vascular Effects 0.000 description 18
- 229910052799 carbon Inorganic materials 0.000 description 17
- 235000013339 cereals Nutrition 0.000 description 16
- 230000000875 corresponding effect Effects 0.000 description 16
- 238000000126 in silico method Methods 0.000 description 16
- 239000002609 medium Substances 0.000 description 16
- 238000010606 normalization Methods 0.000 description 16
- 229910052698 phosphorus Inorganic materials 0.000 description 16
- 239000013612 plasmid Substances 0.000 description 16
- 102100025191 Cyclin-A2 Human genes 0.000 description 15
- 102000009658 Peptidylprolyl Isomerase Human genes 0.000 description 15
- 108010020062 Peptidylprolyl Isomerase Proteins 0.000 description 15
- 108010068192 Cyclin A Proteins 0.000 description 14
- 238000003556 assay Methods 0.000 description 14
- 230000001747 exhibiting effect Effects 0.000 description 14
- 230000008121 plant development Effects 0.000 description 14
- 108020005544 Antisense RNA Proteins 0.000 description 13
- 235000008577 Pinus radiata Nutrition 0.000 description 13
- 239000007788 liquid Substances 0.000 description 13
- 102000005483 Cell Cycle Proteins Human genes 0.000 description 12
- 241001233195 Eucalyptus grandis Species 0.000 description 12
- 101000878213 Homo sapiens Inactive peptidyl-prolyl cis-trans isomerase FKBP6 Proteins 0.000 description 12
- 102100036984 Inactive peptidyl-prolyl cis-trans isomerase FKBP6 Human genes 0.000 description 12
- 150000001413 amino acids Chemical class 0.000 description 12
- 230000007613 environmental effect Effects 0.000 description 12
- 229910052757 nitrogen Inorganic materials 0.000 description 12
- 239000007787 solid Substances 0.000 description 12
- 241000219194 Arabidopsis Species 0.000 description 11
- 108091007914 CDKs Proteins 0.000 description 11
- 229920001131 Pulp (paper) Polymers 0.000 description 11
- 239000000975 dye Substances 0.000 description 11
- 238000002474 experimental method Methods 0.000 description 11
- 239000012634 fragment Substances 0.000 description 11
- 230000004044 response Effects 0.000 description 11
- 238000013518 transcription Methods 0.000 description 11
- 230000035897 transcription Effects 0.000 description 11
- 108090000246 Histone acetyltransferases Proteins 0.000 description 10
- 102000003893 Histone acetyltransferases Human genes 0.000 description 10
- 229940043378 cyclin-dependent kinase inhibitor Drugs 0.000 description 10
- 230000002018 overexpression Effects 0.000 description 10
- 230000002441 reversible effect Effects 0.000 description 10
- 239000000126 substance Substances 0.000 description 10
- 108010068682 Cyclophilins Proteins 0.000 description 9
- 102000001493 Cyclophilins Human genes 0.000 description 9
- 102000003964 Histone deacetylase Human genes 0.000 description 9
- 108090000353 Histone deacetylase Proteins 0.000 description 9
- 238000012228 RNA interference-mediated gene silencing Methods 0.000 description 9
- 239000003153 chemical reaction reagent Substances 0.000 description 9
- 238000001514 detection method Methods 0.000 description 9
- 230000009368 gene silencing by RNA Effects 0.000 description 9
- 230000008569 process Effects 0.000 description 9
- 230000000644 propagated effect Effects 0.000 description 9
- 244000004281 Eucalyptus maculata Species 0.000 description 8
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 8
- 230000003197 catalytic effect Effects 0.000 description 8
- 238000013461 design Methods 0.000 description 8
- 238000003780 insertion Methods 0.000 description 8
- 230000037431 insertion Effects 0.000 description 8
- 241000589158 Agrobacterium Species 0.000 description 7
- 102100032857 Cyclin-dependent kinase 1 Human genes 0.000 description 7
- 240000004658 Medicago sativa Species 0.000 description 7
- 102100037827 Peptidyl-prolyl cis-trans isomerase D Human genes 0.000 description 7
- 108050006676 Retinoblastoma-related proteins Proteins 0.000 description 7
- 230000003213 activating effect Effects 0.000 description 7
- 239000003184 complementary RNA Substances 0.000 description 7
- 238000012217 deletion Methods 0.000 description 7
- 230000037430 deletion Effects 0.000 description 7
- BRZYSWJRSDMWLG-CAXSIQPQSA-N geneticin Chemical compound O1C[C@@](O)(C)[C@H](NC)[C@@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](C(C)O)O2)N)[C@@H](N)C[C@H]1N BRZYSWJRSDMWLG-CAXSIQPQSA-N 0.000 description 7
- 238000002955 isolation Methods 0.000 description 7
- 230000000394 mitotic effect Effects 0.000 description 7
- 238000012163 sequencing technique Methods 0.000 description 7
- 230000007704 transition Effects 0.000 description 7
- 108020005345 3' Untranslated Regions Proteins 0.000 description 6
- 108091026890 Coding region Proteins 0.000 description 6
- 108020004635 Complementary DNA Proteins 0.000 description 6
- 241000218631 Coniferophyta Species 0.000 description 6
- 108091035707 Consensus sequence Proteins 0.000 description 6
- 101000868333 Homo sapiens Cyclin-dependent kinase 1 Proteins 0.000 description 6
- 101000909198 Homo sapiens DNA polymerase delta catalytic subunit Proteins 0.000 description 6
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 6
- 108010037490 Peptidyl-Prolyl Cis-Trans Isomerase NIMA-Interacting 4 Proteins 0.000 description 6
- 241000218679 Pinus taeda Species 0.000 description 6
- 238000002123 RNA extraction Methods 0.000 description 6
- 238000003491 array Methods 0.000 description 6
- 230000004071 biological effect Effects 0.000 description 6
- 210000000481 breast Anatomy 0.000 description 6
- 230000008859 change Effects 0.000 description 6
- 230000002596 correlated effect Effects 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 6
- 230000003993 interaction Effects 0.000 description 6
- 238000005259 measurement Methods 0.000 description 6
- 230000001404 mediated effect Effects 0.000 description 6
- 238000010208 microarray analysis Methods 0.000 description 6
- 238000002966 oligonucleotide array Methods 0.000 description 6
- 108010044156 peptidyl-prolyl cis-trans isomerase b Proteins 0.000 description 6
- 238000007790 scraping Methods 0.000 description 6
- 108091029845 Aminoallyl nucleotide Proteins 0.000 description 5
- 101100273247 Arabidopsis thaliana KRP1 gene Proteins 0.000 description 5
- 239000002028 Biomass Substances 0.000 description 5
- 108090000994 Catalytic RNA Proteins 0.000 description 5
- 102000053642 Catalytic RNA Human genes 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- 235000008566 Pinus taeda Nutrition 0.000 description 5
- 241000219000 Populus Species 0.000 description 5
- 241000218978 Populus deltoides Species 0.000 description 5
- 239000013614 RNA sample Substances 0.000 description 5
- 229930006000 Sucrose Natural products 0.000 description 5
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 5
- 238000007792 addition Methods 0.000 description 5
- 239000000872 buffer Substances 0.000 description 5
- 230000001351 cycling effect Effects 0.000 description 5
- 101150115248 hda gene Proteins 0.000 description 5
- 101150114736 hit gene Proteins 0.000 description 5
- 238000000338 in vitro Methods 0.000 description 5
- 108091092562 ribozyme Proteins 0.000 description 5
- 150000003839 salts Chemical class 0.000 description 5
- 239000005720 sucrose Substances 0.000 description 5
- 238000011282 treatment Methods 0.000 description 5
- 230000006459 vascular development Effects 0.000 description 5
- 241000796533 Arna Species 0.000 description 4
- 229940126074 CDK kinase inhibitor Drugs 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- 241000701489 Cauliflower mosaic virus Species 0.000 description 4
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- 102100031673 Corneodesmosin Human genes 0.000 description 4
- 102100034770 Cyclin-dependent kinase inhibitor 3 Human genes 0.000 description 4
- 108010053770 Deoxyribonucleases Proteins 0.000 description 4
- 102000016911 Deoxyribonucleases Human genes 0.000 description 4
- 102100031780 Endonuclease Human genes 0.000 description 4
- 230000010190 G1 phase Effects 0.000 description 4
- 101000945639 Homo sapiens Cyclin-dependent kinase inhibitor 3 Proteins 0.000 description 4
- 108010042653 IgA receptor Proteins 0.000 description 4
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 4
- 244000061176 Nicotiana tabacum Species 0.000 description 4
- 108010047956 Nucleosomes Proteins 0.000 description 4
- 102100034014 Prolyl 3-hydroxylase 3 Human genes 0.000 description 4
- 229940096437 Protein S Drugs 0.000 description 4
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 4
- 230000018199 S phase Effects 0.000 description 4
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 4
- 108091023040 Transcription factor Proteins 0.000 description 4
- 102000040945 Transcription factor Human genes 0.000 description 4
- 108010031318 Vitronectin Proteins 0.000 description 4
- 240000008042 Zea mays Species 0.000 description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 4
- 230000033228 biological regulation Effects 0.000 description 4
- 230000002520 cambial effect Effects 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 4
- 238000012512 characterization method Methods 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 238000007906 compression Methods 0.000 description 4
- 230000006835 compression Effects 0.000 description 4
- 238000004590 computer program Methods 0.000 description 4
- 238000005520 cutting process Methods 0.000 description 4
- 239000002875 cyclin dependent kinase inhibitor Substances 0.000 description 4
- 230000006378 damage Effects 0.000 description 4
- 238000006911 enzymatic reaction Methods 0.000 description 4
- 238000001502 gel electrophoresis Methods 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical class O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 4
- 239000011121 hardwood Substances 0.000 description 4
- 238000003306 harvesting Methods 0.000 description 4
- 230000006698 induction Effects 0.000 description 4
- 229960000318 kanamycin Drugs 0.000 description 4
- 229940043355 kinase inhibitor Drugs 0.000 description 4
- 238000007899 nucleic acid hybridization Methods 0.000 description 4
- 210000001623 nucleosome Anatomy 0.000 description 4
- 230000036961 partial effect Effects 0.000 description 4
- 239000003757 phosphotransferase inhibitor Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 108020001580 protein domains Proteins 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- 230000022983 regulation of cell cycle Effects 0.000 description 4
- 239000002689 soil Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 238000010561 standard procedure Methods 0.000 description 4
- 238000006467 substitution reaction Methods 0.000 description 4
- 230000002123 temporal effect Effects 0.000 description 4
- 230000001131 transforming effect Effects 0.000 description 4
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 3
- 241000218642 Abies Species 0.000 description 3
- 102000001805 Bromodomains Human genes 0.000 description 3
- 108050009021 Bromodomains Proteins 0.000 description 3
- 108010058544 Cyclin D2 Proteins 0.000 description 3
- 102000007260 Deoxyribonuclease I Human genes 0.000 description 3
- 108010008532 Deoxyribonuclease I Proteins 0.000 description 3
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
- 108700039887 Essential Genes Proteins 0.000 description 3
- 244000080540 Eucalyptus hybrid Species 0.000 description 3
- 235000006913 Eucalyptus hybrid Nutrition 0.000 description 3
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 3
- 102100022846 Histone acetyltransferase KAT2B Human genes 0.000 description 3
- 102000006947 Histones Human genes 0.000 description 3
- 101001046967 Homo sapiens Histone acetyltransferase KAT2A Proteins 0.000 description 3
- 101001047006 Homo sapiens Histone acetyltransferase KAT2B Proteins 0.000 description 3
- 206010020649 Hyperkeratosis Diseases 0.000 description 3
- 108091092195 Intron Proteins 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 101710163270 Nuclease Proteins 0.000 description 3
- 240000007594 Oryza sativa Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- 239000002202 Polyethylene glycol Substances 0.000 description 3
- 101710150593 Protein beta Proteins 0.000 description 3
- 201000000582 Retinoblastoma Diseases 0.000 description 3
- 238000012300 Sequence Analysis Methods 0.000 description 3
- 208000037065 Subacute sclerosing leukoencephalitis Diseases 0.000 description 3
- 206010042297 Subacute sclerosing panencephalitis Diseases 0.000 description 3
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 3
- 239000004473 Threonine Substances 0.000 description 3
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 3
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 3
- 230000021736 acetylation Effects 0.000 description 3
- 238000006640 acetylation reaction Methods 0.000 description 3
- 102000005421 acetyltransferase Human genes 0.000 description 3
- 108020002494 acetyltransferase Proteins 0.000 description 3
- 230000004913 activation Effects 0.000 description 3
- 239000011543 agarose gel Substances 0.000 description 3
- 230000004075 alteration Effects 0.000 description 3
- 125000000539 amino acid group Chemical group 0.000 description 3
- 238000012197 amplification kit Methods 0.000 description 3
- 210000003050 axon Anatomy 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 3
- 230000001413 cellular effect Effects 0.000 description 3
- 210000003763 chloroplast Anatomy 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 108010072268 cyclin-dependent kinase-activating kinase Proteins 0.000 description 3
- 238000007405 data analysis Methods 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 239000000499 gel Substances 0.000 description 3
- 102000054767 gene variant Human genes 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 230000006197 histone deacetylation Effects 0.000 description 3
- 238000011065 in-situ storage Methods 0.000 description 3
- 229930027917 kanamycin Natural products 0.000 description 3
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 3
- 229930182823 kanamycin A Natural products 0.000 description 3
- 238000002372 labelling Methods 0.000 description 3
- 235000009973 maize Nutrition 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 230000011278 mitosis Effects 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 239000003147 molecular marker Substances 0.000 description 3
- 239000013642 negative control Substances 0.000 description 3
- 230000026731 phosphorylation Effects 0.000 description 3
- 238000006366 phosphorylation reaction Methods 0.000 description 3
- 229920001223 polyethylene glycol Polymers 0.000 description 3
- 230000017854 proteolysis Effects 0.000 description 3
- 230000035939 shock Effects 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 238000013519 translation Methods 0.000 description 3
- 210000005166 vasculature Anatomy 0.000 description 3
- 229910052725 zinc Inorganic materials 0.000 description 3
- 239000011701 zinc Substances 0.000 description 3
- ZTTWHZHBPDYSQB-LBPRGKRZSA-N (2s)-2-amino-3-(1h-indol-3-yl)-2-methylpropanoic acid Chemical compound C1=CC=C2C(C[C@@](N)(C)C(O)=O)=CNC2=C1 ZTTWHZHBPDYSQB-LBPRGKRZSA-N 0.000 description 2
- BZTDTCNHAFUJOG-UHFFFAOYSA-N 6-carboxyfluorescein Chemical compound C12=CC=C(O)C=C2OC2=CC(O)=CC=C2C11OC(=O)C2=CC=C(C(=O)O)C=C21 BZTDTCNHAFUJOG-UHFFFAOYSA-N 0.000 description 2
- FVFVNNKYKYZTJU-UHFFFAOYSA-N 6-chloro-1,3,5-triazine-2,4-diamine Chemical compound NC1=NC(N)=NC(Cl)=N1 FVFVNNKYKYZTJU-UHFFFAOYSA-N 0.000 description 2
- 240000005020 Acaciella glauca Species 0.000 description 2
- 241000208140 Acer Species 0.000 description 2
- 108010031677 Anaphase-Promoting Complex-Cyclosome Proteins 0.000 description 2
- 102000005446 Anaphase-Promoting Complex-Cyclosome Human genes 0.000 description 2
- 101000651036 Arabidopsis thaliana Galactolipid galactosyltransferase SFR2, chloroplastic Proteins 0.000 description 2
- 101001083371 Arabidopsis thaliana Histone acetyltransferase type B catalytic subunit Proteins 0.000 description 2
- 229930192334 Auxin Natural products 0.000 description 2
- 101150023302 Cdc20 gene Proteins 0.000 description 2
- 102000017589 Chromo domains Human genes 0.000 description 2
- 108050005811 Chromo domains Proteins 0.000 description 2
- 238000000018 DNA microarray Methods 0.000 description 2
- 230000004543 DNA replication Effects 0.000 description 2
- 101100210292 Drosophila melanogaster Wee1 gene Proteins 0.000 description 2
- 244000239638 Eucalyptus bridgesiana Species 0.000 description 2
- 235000005224 Eucalyptus bridgesiana Nutrition 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- 235000016623 Fragaria vesca Nutrition 0.000 description 2
- 240000009088 Fragaria x ananassa Species 0.000 description 2
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 102100024185 G1/S-specific cyclin-D2 Human genes 0.000 description 2
- 108700007698 Genetic Terminator Regions Proteins 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- YIWFXZNIBQBFHR-UHFFFAOYSA-N Glycyl-Histidine Chemical compound NCC(=O)NC(C(O)=O)CC1=CN=CN1 YIWFXZNIBQBFHR-UHFFFAOYSA-N 0.000 description 2
- 101000621390 Homo sapiens Wee1-like protein kinase Proteins 0.000 description 2
- 241000218652 Larix Species 0.000 description 2
- 235000005590 Larix decidua Nutrition 0.000 description 2
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 2
- 239000004472 Lysine Substances 0.000 description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
- 108091027974 Mature messenger RNA Proteins 0.000 description 2
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 2
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 2
- 108700026244 Open Reading Frames Proteins 0.000 description 2
- 101000898974 Oryza sativa subsp. japonica Probable histone acetyltransferase type B catalytic subunit Proteins 0.000 description 2
- 108090000854 Oxidoreductases Proteins 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- 235000008124 Picea excelsa Nutrition 0.000 description 2
- 240000000020 Picea glauca Species 0.000 description 2
- 235000008127 Picea glauca Nutrition 0.000 description 2
- 235000008582 Pinus sylvestris Nutrition 0.000 description 2
- 108700001094 Plant Genes Proteins 0.000 description 2
- 108010029485 Protein Isoforms Proteins 0.000 description 2
- 102000001708 Protein Isoforms Human genes 0.000 description 2
- 241000219492 Quercus Species 0.000 description 2
- 108091034057 RNA (poly(A)) Proteins 0.000 description 2
- 102000015097 RNA Splicing Factors Human genes 0.000 description 2
- 108010039259 RNA Splicing Factors Proteins 0.000 description 2
- 102100038042 Retinoblastoma-associated protein Human genes 0.000 description 2
- 101710124357 Retinoblastoma-associated protein Proteins 0.000 description 2
- 102000006382 Ribonucleases Human genes 0.000 description 2
- 108010083644 Ribonucleases Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 240000003768 Solanum lycopersicum Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 244000098338 Triticum aestivum Species 0.000 description 2
- 102100023037 Wee1-like protein kinase Human genes 0.000 description 2
- 241000209149 Zea Species 0.000 description 2
- OJOBTAOGJIWAGB-UHFFFAOYSA-N acetosyringone Chemical compound COC1=CC(C(C)=O)=CC(OC)=C1O OJOBTAOGJIWAGB-UHFFFAOYSA-N 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 238000002820 assay format Methods 0.000 description 2
- 239000002363 auxin Substances 0.000 description 2
- 238000012742 biochemical analysis Methods 0.000 description 2
- 238000010256 biochemical assay Methods 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 230000006369 cell cycle progression Effects 0.000 description 2
- 210000000349 chromosome Anatomy 0.000 description 2
- 238000012790 confirmation Methods 0.000 description 2
- 239000000356 contaminant Substances 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 210000004292 cytoskeleton Anatomy 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 230000000368 destabilizing effect Effects 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 239000001177 diphosphate Substances 0.000 description 2
- 230000003828 downregulation Effects 0.000 description 2
- 230000005014 ectopic expression Effects 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 210000002257 embryonic structure Anatomy 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 238000001952 enzyme assay Methods 0.000 description 2
- 238000012869 ethanol precipitation Methods 0.000 description 2
- 230000005078 fruit development Effects 0.000 description 2
- 230000004345 fruit ripening Effects 0.000 description 2
- 238000011223 gene expression profiling Methods 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 239000003112 inhibitor Substances 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 239000006166 lysate Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000000442 meristematic effect Effects 0.000 description 2
- 238000007431 microscopic evaluation Methods 0.000 description 2
- 238000000386 microscopy Methods 0.000 description 2
- 239000003068 molecular probe Substances 0.000 description 2
- 239000002853 nucleic acid probe Substances 0.000 description 2
- 230000000704 physical effect Effects 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 230000010152 pollination Effects 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 230000032361 posttranscriptional gene silencing Effects 0.000 description 2
- 239000002243 precursor Substances 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000001742 protein purification Methods 0.000 description 2
- 230000002285 radioactive effect Effects 0.000 description 2
- 238000003753 real-time PCR Methods 0.000 description 2
- 230000010076 replication Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 239000012882 rooting medium Substances 0.000 description 2
- -1 rotanase Proteins 0.000 description 2
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 2
- 238000005204 segregation Methods 0.000 description 2
- 238000002864 sequence alignment Methods 0.000 description 2
- 235000021309 simple sugar Nutrition 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 229940027257 timentin Drugs 0.000 description 2
- UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 description 2
- UZKQTCBAMSWPJD-FARCUNLSSA-N trans-zeatin Chemical compound OCC(/C)=C/CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-FARCUNLSSA-N 0.000 description 2
- 230000037426 transcriptional repression Effects 0.000 description 2
- PEEAINPHPNDNGE-UHFFFAOYSA-N tryptophyl-aspartate Chemical compound C1=CC=C2C(CC(N)C(=O)NC(CC(O)=O)C(O)=O)=CNC2=C1 PEEAINPHPNDNGE-UHFFFAOYSA-N 0.000 description 2
- 238000000539 two dimensional gel electrophoresis Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 229940023877 zeatin Drugs 0.000 description 2
- GEWDNTWNSAZUDX-WQMVXFAESA-N (-)-methyl jasmonate Chemical compound CC\C=C/C[C@@H]1[C@@H](CC(=O)OC)CCC1=O GEWDNTWNSAZUDX-WQMVXFAESA-N 0.000 description 1
- IXVMHGVQKLDRKH-YEJCTVDLSA-N (22s,23s)-epibrassinolide Chemical compound C1OC(=O)[C@H]2C[C@H](O)[C@H](O)C[C@]2(C)[C@H]2CC[C@]3(C)[C@@H]([C@H](C)[C@H](O)[C@@H](O)[C@H](C)C(C)C)CC[C@H]3[C@@H]21 IXVMHGVQKLDRKH-YEJCTVDLSA-N 0.000 description 1
- VUFNLQXQSDUXKB-DOFZRALJSA-N 2-[4-[4-[bis(2-chloroethyl)amino]phenyl]butanoyloxy]ethyl (5z,8z,11z,14z)-icosa-5,8,11,14-tetraenoate Chemical compound CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(=O)OCCOC(=O)CCCC1=CC=C(N(CCCl)CCCl)C=C1 VUFNLQXQSDUXKB-DOFZRALJSA-N 0.000 description 1
- JRYMOPZHXMVHTA-DAGMQNCNSA-N 2-amino-7-[(2r,3r,4s,5r)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-1h-pyrrolo[2,3-d]pyrimidin-4-one Chemical compound C1=CC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O JRYMOPZHXMVHTA-DAGMQNCNSA-N 0.000 description 1
- JLIDBLDQVAYHNE-LXGGSRJLSA-N 2-cis-abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\C1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-LXGGSRJLSA-N 0.000 description 1
- IXVMHGVQKLDRKH-QHBHMFGVSA-N 24-epi-brassinolide Chemical compound C1OC(=O)[C@H]2C[C@H](O)[C@H](O)C[C@]2(C)[C@H]2CC[C@]3(C)[C@@H]([C@H](C)[C@@H](O)[C@H](O)[C@H](C)C(C)C)CC[C@H]3[C@@H]21 IXVMHGVQKLDRKH-QHBHMFGVSA-N 0.000 description 1
- 108010037497 3'-nucleotidase Proteins 0.000 description 1
- CAAMSDWKXXPUJR-UHFFFAOYSA-N 3,5-dihydro-4H-imidazol-4-one Chemical compound O=C1CNC=N1 CAAMSDWKXXPUJR-UHFFFAOYSA-N 0.000 description 1
- 108010020183 3-phosphoshikimate 1-carboxyvinyltransferase Proteins 0.000 description 1
- COCMHKNAGZHBDZ-UHFFFAOYSA-N 4-carboxy-3-[3-(dimethylamino)-6-dimethylazaniumylidenexanthen-9-yl]benzoate Chemical compound C=12C=CC(=[N+](C)C)C=C2OC2=CC(N(C)C)=CC=C2C=1C1=CC(C([O-])=O)=CC=C1C(O)=O COCMHKNAGZHBDZ-UHFFFAOYSA-N 0.000 description 1
- 101150096316 5 gene Proteins 0.000 description 1
- 108020005029 5' Flanking Region Proteins 0.000 description 1
- OPIFSICVWOWJMJ-AEOCFKNESA-N 5-bromo-4-chloro-3-indolyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC=C(Br)C(Cl)=C12 OPIFSICVWOWJMJ-AEOCFKNESA-N 0.000 description 1
- 101150039504 6 gene Proteins 0.000 description 1
- JXCKZXHCJOVIAV-UHFFFAOYSA-N 6-[(5-bromo-4-chloro-1h-indol-3-yl)oxy]-3,4,5-trihydroxyoxane-2-carboxylic acid;cyclohexanamine Chemical compound [NH3+]C1CCCCC1.O1C(C([O-])=O)C(O)C(O)C(O)C1OC1=CNC2=CC=C(Br)C(Cl)=C12 JXCKZXHCJOVIAV-UHFFFAOYSA-N 0.000 description 1
- 108010022579 ATP dependent 26S protease Proteins 0.000 description 1
- 244000283070 Abies balsamea Species 0.000 description 1
- 235000007173 Abies balsamea Nutrition 0.000 description 1
- 241000379228 Abies concolor Species 0.000 description 1
- 235000017894 Abies grandis Nutrition 0.000 description 1
- 244000178606 Abies grandis Species 0.000 description 1
- 244000166033 Abies lasiocarpa Species 0.000 description 1
- 235000004710 Abies lasiocarpa Nutrition 0.000 description 1
- 241000379225 Abies procera Species 0.000 description 1
- 108010000700 Acetolactate synthase Proteins 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 108010039224 Amidophosphoribosyltransferase Proteins 0.000 description 1
- 244000105975 Antidesma platyphyllum Species 0.000 description 1
- 241000219195 Arabidopsis thaliana Species 0.000 description 1
- 101100007506 Arabidopsis thaliana CYP20-1 gene Proteins 0.000 description 1
- 101500006437 Arabidopsis thaliana Ubiquitin Proteins 0.000 description 1
- 244000075850 Avena orientalis Species 0.000 description 1
- 235000007319 Avena orientalis Nutrition 0.000 description 1
- 235000007558 Avena sp Nutrition 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000219310 Beta vulgaris subsp. vulgaris Species 0.000 description 1
- 241000212384 Bifora Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- IXVMHGVQKLDRKH-VRESXRICSA-N Brassinolide Natural products O=C1OC[C@@H]2[C@@H]3[C@@](C)([C@H]([C@@H]([C@@H](O)[C@H](O)[C@H](C(C)C)C)C)CC3)CC[C@@H]2[C@]2(C)[C@@H]1C[C@H](O)[C@H](O)C2 IXVMHGVQKLDRKH-VRESXRICSA-N 0.000 description 1
- 101150047289 CDC2C gene Proteins 0.000 description 1
- 101150012716 CDK1 gene Proteins 0.000 description 1
- 101100252357 Caenorhabditis elegans rnp-1 gene Proteins 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical group [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241001674345 Callitropsis nootkatensis Species 0.000 description 1
- 241000722694 Calocedrus decurrens Species 0.000 description 1
- 235000003571 Camden woollybutt Nutrition 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- 241000723437 Chamaecyparis Species 0.000 description 1
- 241001117253 Chamaecyparis thyoides Species 0.000 description 1
- 102100031065 Choline kinase alpha Human genes 0.000 description 1
- 108010077544 Chromatin Proteins 0.000 description 1
- 102000008169 Co-Repressor Proteins Human genes 0.000 description 1
- 108010060434 Co-Repressor Proteins Proteins 0.000 description 1
- 102000057710 Coatomer Human genes 0.000 description 1
- 108700022408 Coatomer Proteins 0.000 description 1
- 241000037488 Coccoloba pubescens Species 0.000 description 1
- 241001480079 Corymbia calophylla Species 0.000 description 1
- 244000107602 Corymbia citriodora Species 0.000 description 1
- 241001480081 Corymbia ficifolia Species 0.000 description 1
- 241001399644 Corymbia torelliana Species 0.000 description 1
- 108010060273 Cyclin A2 Proteins 0.000 description 1
- 102000002495 Cyclin H Human genes 0.000 description 1
- 108010068237 Cyclin H Proteins 0.000 description 1
- 108010048028 Cyclophilin D Proteins 0.000 description 1
- PMATZTZNYRCHOR-CGLBZJNRSA-N Cyclosporin A Chemical compound CC[C@@H]1NC(=O)[C@H]([C@H](O)[C@H](C)C\C=C\C)N(C)C(=O)[C@H](C(C)C)N(C)C(=O)[C@H](CC(C)C)N(C)C(=O)[C@H](CC(C)C)N(C)C(=O)[C@@H](C)NC(=O)[C@H](C)NC(=O)[C@H](CC(C)C)N(C)C(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)N(C)C(=O)CN(C)C1=O PMATZTZNYRCHOR-CGLBZJNRSA-N 0.000 description 1
- 229930105110 Cyclosporin A Natural products 0.000 description 1
- 108010036949 Cyclosporine Proteins 0.000 description 1
- 108010066133 D-octopine dehydrogenase Proteins 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 230000004568 DNA-binding Effects 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 235000002767 Daucus carota Nutrition 0.000 description 1
- 244000000626 Daucus carota Species 0.000 description 1
- 102100037922 Disco-interacting protein 2 homolog A Human genes 0.000 description 1
- 101100005779 Drosophila melanogaster Cdk2 gene Proteins 0.000 description 1
- 235000000066 Eilar pine Nutrition 0.000 description 1
- 101100059559 Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) nimX gene Proteins 0.000 description 1
- 101100288094 Escherichia coli aphA1 gene Proteins 0.000 description 1
- 244000187785 Eucalyptus alba Species 0.000 description 1
- 235000004722 Eucalyptus citriodora Nutrition 0.000 description 1
- 240000004181 Eucalyptus cladocalyx Species 0.000 description 1
- 241000006105 Eucalyptus coccifera Species 0.000 description 1
- 241001480120 Eucalyptus curtisii Species 0.000 description 1
- 241000006108 Eucalyptus dalrympleana Species 0.000 description 1
- 241001528275 Eucalyptus deglupta Species 0.000 description 1
- 241000396461 Eucalyptus diversicolor Species 0.000 description 1
- 241001074688 Eucalyptus dunnii Species 0.000 description 1
- 235000004692 Eucalyptus globulus Nutrition 0.000 description 1
- 235000000018 Eucalyptus gomphocephala Nutrition 0.000 description 1
- 244000210004 Eucalyptus gomphocephala Species 0.000 description 1
- 235000004504 Eucalyptus gunnii Nutrition 0.000 description 1
- 244000059939 Eucalyptus gunnii Species 0.000 description 1
- 241001659093 Eucalyptus laevopinea Species 0.000 description 1
- 241001639228 Eucalyptus macarthurii Species 0.000 description 1
- 241000037027 Eucalyptus macrorhyncha Species 0.000 description 1
- 235000010705 Eucalyptus maculata Nutrition 0.000 description 1
- 241001074671 Eucalyptus marginata Species 0.000 description 1
- 241001074674 Eucalyptus megacarpa Species 0.000 description 1
- 240000003845 Eucalyptus nicholii Species 0.000 description 1
- 235000003543 Eucalyptus nicholii Nutrition 0.000 description 1
- 241000006114 Eucalyptus nitens Species 0.000 description 1
- 241001506770 Eucalyptus obliqua Species 0.000 description 1
- 241001074711 Eucalyptus obtusiflora Species 0.000 description 1
- 241000333074 Eucalyptus occidentalis Species 0.000 description 1
- 240000008967 Eucalyptus oreades Species 0.000 description 1
- 235000002003 Eucalyptus oreades Nutrition 0.000 description 1
- 241000006116 Eucalyptus pauciflora Species 0.000 description 1
- 240000004476 Eucalyptus polybractea Species 0.000 description 1
- 235000009683 Eucalyptus polybractea Nutrition 0.000 description 1
- 241000006121 Eucalyptus regnans Species 0.000 description 1
- 244000103090 Eucalyptus robusta Species 0.000 description 1
- 241000400623 Eucalyptus rudis Species 0.000 description 1
- 240000006361 Eucalyptus saligna Species 0.000 description 1
- 241001541931 Eucalyptus sideroxylon Species 0.000 description 1
- 235000005231 Eucalyptus sideroxylon Nutrition 0.000 description 1
- 235000013378 Eucalyptus stuartiana Nutrition 0.000 description 1
- 235000019134 Eucalyptus tereticornis Nutrition 0.000 description 1
- 240000007002 Eucalyptus tereticornis Species 0.000 description 1
- 241001182996 Eucalyptus urnigera Species 0.000 description 1
- 241000404037 Eucalyptus urophylla Species 0.000 description 1
- 235000013366 Eucalyptus viminalis Nutrition 0.000 description 1
- 240000001414 Eucalyptus viminalis Species 0.000 description 1
- 240000001716 Eucalyptus viridis Species 0.000 description 1
- 235000003458 Eucalyptus viridis Nutrition 0.000 description 1
- 241001074695 Eucalyptus wandoo Species 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 235000015569 Falconbridge mallee ash Nutrition 0.000 description 1
- 230000010337 G2 phase Effects 0.000 description 1
- 241000208152 Geranium Species 0.000 description 1
- 229930191978 Gibberellin Natural products 0.000 description 1
- 102000053187 Glucuronidase Human genes 0.000 description 1
- 108010060309 Glucuronidase Proteins 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 239000005562 Glyphosate Substances 0.000 description 1
- 102000009331 Homeodomain Proteins Human genes 0.000 description 1
- 108010048671 Homeodomain Proteins Proteins 0.000 description 1
- 101100059561 Homo sapiens CDK1 gene Proteins 0.000 description 1
- 101000805876 Homo sapiens Disco-interacting protein 2 homolog A Proteins 0.000 description 1
- 101001006782 Homo sapiens Kinesin-associated protein 3 Proteins 0.000 description 1
- 101001030232 Homo sapiens Myosin-9 Proteins 0.000 description 1
- 101000603223 Homo sapiens Nischarin Proteins 0.000 description 1
- 101001059454 Homo sapiens Serine/threonine-protein kinase MARK2 Proteins 0.000 description 1
- 101001090074 Homo sapiens Small nuclear protein PRAC1 Proteins 0.000 description 1
- 101000753286 Homo sapiens Transcription intermediary factor 1-beta Proteins 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- 229930010555 Inosine Natural products 0.000 description 1
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 1
- 244000017020 Ipomoea batatas Species 0.000 description 1
- 235000002678 Ipomoea batatas Nutrition 0.000 description 1
- 241001527806 Iti Species 0.000 description 1
- 108010025815 Kanamycin Kinase Proteins 0.000 description 1
- 235000003228 Lactuca sativa Nutrition 0.000 description 1
- 240000008415 Lactuca sativa Species 0.000 description 1
- 241001235216 Larix decidua Species 0.000 description 1
- 241000534018 Larix kaempferi Species 0.000 description 1
- 241000218653 Larix laricina Species 0.000 description 1
- 235000008119 Larix laricina Nutrition 0.000 description 1
- 235000008122 Larix occidentalis Nutrition 0.000 description 1
- 244000193510 Larix occidentalis Species 0.000 description 1
- 240000007472 Leucaena leucocephala Species 0.000 description 1
- 235000010643 Leucaena leucocephala Nutrition 0.000 description 1
- 241000208682 Liquidambar Species 0.000 description 1
- 235000006552 Liquidambar styraciflua Nutrition 0.000 description 1
- 206010048911 Lissencephaly Diseases 0.000 description 1
- 108700012928 MAPK14 Proteins 0.000 description 1
- 241000218922 Magnoliophyta Species 0.000 description 1
- 240000003183 Manihot esculenta Species 0.000 description 1
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 108091022875 Microtubule Proteins 0.000 description 1
- 102000029749 Microtubule Human genes 0.000 description 1
- 108010085220 Multiprotein Complexes Proteins 0.000 description 1
- 102000007474 Multiprotein Complexes Human genes 0.000 description 1
- 102100038938 Myosin-9 Human genes 0.000 description 1
- 238000005481 NMR spectroscopy Methods 0.000 description 1
- 101710116139 Negative regulator of mitosis Proteins 0.000 description 1
- 102100038995 Nischarin Human genes 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- 108091005461 Nucleic proteins Proteins 0.000 description 1
- 240000008881 Oenanthe javanica Species 0.000 description 1
- 206010034133 Pathogen resistance Diseases 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091093037 Peptide nucleic acid Proteins 0.000 description 1
- 241000042032 Petrocephalus catostoma Species 0.000 description 1
- 241000218657 Picea Species 0.000 description 1
- 241000218597 Picea engelmannii Species 0.000 description 1
- 240000009002 Picea mariana Species 0.000 description 1
- 235000008145 Picea mariana Nutrition 0.000 description 1
- 241000218594 Picea pungens Species 0.000 description 1
- 241000218596 Picea rubens Species 0.000 description 1
- 241000218595 Picea sitchensis Species 0.000 description 1
- 235000008565 Pinus banksiana Nutrition 0.000 description 1
- 241000218680 Pinus banksiana Species 0.000 description 1
- 241001206823 Pinus brutia var. eldarica Species 0.000 description 1
- 235000009324 Pinus caribaea Nutrition 0.000 description 1
- 241001223353 Pinus caribaea Species 0.000 description 1
- 235000013431 Pinus clausa Nutrition 0.000 description 1
- 241000048268 Pinus clausa Species 0.000 description 1
- 235000008593 Pinus contorta Nutrition 0.000 description 1
- 241000218606 Pinus contorta Species 0.000 description 1
- 235000008568 Pinus coulteri Nutrition 0.000 description 1
- 244000083281 Pinus coulteri Species 0.000 description 1
- 235000005018 Pinus echinata Nutrition 0.000 description 1
- 241001236219 Pinus echinata Species 0.000 description 1
- 235000013264 Pinus jeffreyi Nutrition 0.000 description 1
- 244000019397 Pinus jeffreyi Species 0.000 description 1
- 235000008595 Pinus lambertiana Nutrition 0.000 description 1
- 240000008299 Pinus lambertiana Species 0.000 description 1
- 235000011609 Pinus massoniana Nutrition 0.000 description 1
- 241000018650 Pinus massoniana Species 0.000 description 1
- 241000218617 Pinus monticola Species 0.000 description 1
- 235000016421 Pinus nigra Nutrition 0.000 description 1
- 241000592226 Pinus nigra Species 0.000 description 1
- 235000017339 Pinus palustris Nutrition 0.000 description 1
- 241000204936 Pinus palustris Species 0.000 description 1
- 235000005105 Pinus pinaster Nutrition 0.000 description 1
- 241001236212 Pinus pinaster Species 0.000 description 1
- 235000013267 Pinus ponderosa Nutrition 0.000 description 1
- 241000555277 Pinus ponderosa Species 0.000 description 1
- 235000013697 Pinus resinosa Nutrition 0.000 description 1
- 241000534656 Pinus resinosa Species 0.000 description 1
- 235000007738 Pinus rigida Nutrition 0.000 description 1
- 241000369901 Pinus rigida Species 0.000 description 1
- 235000018999 Pinus serotina Nutrition 0.000 description 1
- 241001139411 Pinus serotina Species 0.000 description 1
- 235000008578 Pinus strobus Nutrition 0.000 description 1
- 240000007320 Pinus strobus Species 0.000 description 1
- 235000005103 Pinus virginiana Nutrition 0.000 description 1
- 241001236196 Pinus virginiana Species 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 241000217825 Populus tremula x Populus tremuloides Species 0.000 description 1
- 108010043400 Protamine Kinase Proteins 0.000 description 1
- 108010026552 Proteome Proteins 0.000 description 1
- 241001430696 Protis Species 0.000 description 1
- 102000012500 Proto-Oncogene Proteins c-crk Human genes 0.000 description 1
- 235000008572 Pseudotsuga menziesii Nutrition 0.000 description 1
- 240000001416 Pseudotsuga menziesii Species 0.000 description 1
- 230000004570 RNA-binding Effects 0.000 description 1
- 238000011530 RNeasy Mini Kit Methods 0.000 description 1
- 101150000851 ROC7 gene Proteins 0.000 description 1
- 102000016812 Radical SAM Human genes 0.000 description 1
- 108050006523 Radical SAM Proteins 0.000 description 1
- 108700008625 Reporter Genes Proteins 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 244000004774 Sabina virginiana Species 0.000 description 1
- 235000008691 Sabina virginiana Nutrition 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000235347 Schizosaccharomyces pombe Species 0.000 description 1
- 241001138418 Sequoia sempervirens Species 0.000 description 1
- 241000422846 Sequoiadendron giganteum Species 0.000 description 1
- 102100028904 Serine/threonine-protein kinase MARK2 Human genes 0.000 description 1
- 108020004459 Small interfering RNA Proteins 0.000 description 1
- 102100034766 Small nuclear protein PRAC1 Human genes 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 235000021536 Sugar beet Nutrition 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- 241001138405 Taxodium distichum Species 0.000 description 1
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
- 108091036066 Three prime untranslated region Proteins 0.000 description 1
- 235000008109 Thuja occidentalis Nutrition 0.000 description 1
- 240000003243 Thuja occidentalis Species 0.000 description 1
- 241000218638 Thuja plicata Species 0.000 description 1
- 241000592342 Tracheophyta Species 0.000 description 1
- 102100022012 Transcription intermediary factor 1-beta Human genes 0.000 description 1
- 102000006612 Transducin Human genes 0.000 description 1
- 108010087042 Transducin Proteins 0.000 description 1
- RTKIYFITIVXBLE-UHFFFAOYSA-N Trichostatin A Natural products ONC(=O)C=CC(C)=CC(C)C(=O)C1=CC=C(N(C)C)C=C1 RTKIYFITIVXBLE-UHFFFAOYSA-N 0.000 description 1
- 240000003021 Tsuga heterophylla Species 0.000 description 1
- 235000008554 Tsuga heterophylla Nutrition 0.000 description 1
- 235000010183 Tsuga mertensiana Nutrition 0.000 description 1
- 240000005004 Tsuga mertensiana Species 0.000 description 1
- HSCJRCZFDFQWRP-JZMIEXBBSA-N UDP-alpha-D-glucose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OP(O)(=O)OP(O)(=O)OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(NC(=O)C=C2)=O)O1 HSCJRCZFDFQWRP-JZMIEXBBSA-N 0.000 description 1
- 102000006275 Ubiquitin-Protein Ligases Human genes 0.000 description 1
- 108010083111 Ubiquitin-Protein Ligases Proteins 0.000 description 1
- 229910052770 Uranium Inorganic materials 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 101100273808 Xenopus laevis cdk1-b gene Proteins 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 101710185494 Zinc finger protein Proteins 0.000 description 1
- 102100023597 Zinc finger protein 816 Human genes 0.000 description 1
- 230000001594 aberrant effect Effects 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 description 1
- 239000008186 active pharmaceutical agent Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- KAOMOVYHGLSFHQ-UTOQUPLUSA-N anacardic acid Chemical compound CCC\C=C/C\C=C/CCCCCCCC1=CC=CC(O)=C1C(O)=O KAOMOVYHGLSFHQ-UTOQUPLUSA-N 0.000 description 1
- 235000014398 anacardic acid Nutrition 0.000 description 1
- ADFWQBGTDJIESE-UHFFFAOYSA-N anacardic acid 15:0 Natural products CCCCCCCCCCCCCCCC1=CC=CC(O)=C1C(O)=O ADFWQBGTDJIESE-UHFFFAOYSA-N 0.000 description 1
- 238000000540 analysis of variance Methods 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 238000000376 autoradiography Methods 0.000 description 1
- 230000001042 autoregulative effect Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000037429 base substitution Effects 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 230000001851 biosynthetic effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000006664 bond formation reaction Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 125000001246 bromo group Chemical group Br* 0.000 description 1
- 238000010804 cDNA synthesis Methods 0.000 description 1
- 238000010805 cDNA synthesis kit Methods 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 230000003915 cell function Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 230000011748 cell maturation Effects 0.000 description 1
- 230000003196 chaotropic effect Effects 0.000 description 1
- 210000003483 chromatin Anatomy 0.000 description 1
- 230000010428 chromatin condensation Effects 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 229960001265 ciclosporin Drugs 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000000975 co-precipitation Methods 0.000 description 1
- 230000022472 cold acclimation Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000006854 communication Effects 0.000 description 1
- 108091036078 conserved sequence Proteins 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 229930182912 cyclosporin Natural products 0.000 description 1
- 230000021953 cytokinesis Effects 0.000 description 1
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 1
- 239000004062 cytokinin Substances 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 238000003381 deacetylation reaction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 description 1
- 235000011180 diphosphates Nutrition 0.000 description 1
- 235000021186 dishes Nutrition 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 238000000572 ellipsometry Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000006353 environmental stress Effects 0.000 description 1
- 230000008029 eradication Effects 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 230000031376 exit from mitosis Effects 0.000 description 1
- 108010052305 exodeoxyribonuclease III Proteins 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 206010016256 fatigue Diseases 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 238000007380 fibre production Methods 0.000 description 1
- 238000002875 fluorescence polarization Methods 0.000 description 1
- 238000001506 fluorescence spectroscopy Methods 0.000 description 1
- 230000037433 frameshift Effects 0.000 description 1
- 230000030279 gene silencing Effects 0.000 description 1
- 238000012226 gene silencing method Methods 0.000 description 1
- 102000034356 gene-regulatory proteins Human genes 0.000 description 1
- 108091006104 gene-regulatory proteins Proteins 0.000 description 1
- 238000003205 genotyping method Methods 0.000 description 1
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 1
- 239000003448 gibberellin Substances 0.000 description 1
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 1
- 229940097068 glyphosate Drugs 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 235000009424 haa Nutrition 0.000 description 1
- 239000000833 heterodimer Substances 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 230000006195 histone acetylation Effects 0.000 description 1
- 230000003054 hormonal effect Effects 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 101150090192 how gene Proteins 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000003119 immunoblot Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 238000002129 infrared reflectance spectroscopy Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 229960003786 inosine Drugs 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 239000000543 intermediate Substances 0.000 description 1
- 238000001155 isoelectric focusing Methods 0.000 description 1
- 238000003674 kinase activity assay Methods 0.000 description 1
- 238000011005 laboratory method Methods 0.000 description 1
- 101150066555 lacZ gene Proteins 0.000 description 1
- 150000002605 large molecules Chemical class 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 238000000670 ligand binding assay Methods 0.000 description 1
- 238000001638 lipofection Methods 0.000 description 1
- 208000014817 lissencephaly spectrum disease Diseases 0.000 description 1
- 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- 238000010841 mRNA extraction Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 239000012092 media component Substances 0.000 description 1
- 230000031864 metaphase Effects 0.000 description 1
- GEWDNTWNSAZUDX-UHFFFAOYSA-N methyl 7-epi-jasmonate Natural products CCC=CCC1C(CC(=O)OC)CCC1=O GEWDNTWNSAZUDX-UHFFFAOYSA-N 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 210000004688 microtubule Anatomy 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 238000000491 multivariate analysis Methods 0.000 description 1
- 210000004897 n-terminal region Anatomy 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- 108010058731 nopaline synthase Proteins 0.000 description 1
- 210000000633 nuclear envelope Anatomy 0.000 description 1
- 238000001216 nucleic acid method Methods 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 235000021232 nutrient availability Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000002515 oligonucleotide synthesis Methods 0.000 description 1
- 238000010397 one-hybrid screening Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 239000008194 pharmaceutical composition Substances 0.000 description 1
- 150000004713 phosphodiesters Chemical class 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 239000001839 pinus sylvestris Substances 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 239000005648 plant growth regulator Substances 0.000 description 1
- 230000004983 pleiotropic effect Effects 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 230000001323 posttranslational effect Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 239000002987 primer (paints) Substances 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 238000003498 protein array Methods 0.000 description 1
- 238000012514 protein characterization Methods 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 238000002331 protein detection Methods 0.000 description 1
- 230000012846 protein folding Effects 0.000 description 1
- 230000004853 protein function Effects 0.000 description 1
- 238000001814 protein method Methods 0.000 description 1
- 230000004850 protein–protein interaction Effects 0.000 description 1
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 description 1
- 235000019171 pyridoxine hydrochloride Nutrition 0.000 description 1
- 239000011764 pyridoxine hydrochloride Substances 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 238000009790 rate-determining step (RDS) Methods 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 235000003566 red stringybark Nutrition 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000007363 regulatory process Effects 0.000 description 1
- 238000007634 remodeling Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 230000002786 root growth Effects 0.000 description 1
- 229960004889 salicylic acid Drugs 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 238000004626 scanning electron microscopy Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- BTIHMVBBUGXLCJ-OAHLLOKOSA-N seliciclib Chemical compound C=12N=CN(C(C)C)C2=NC(N[C@@H](CO)CC)=NC=1NCC1=CC=CC=C1 BTIHMVBBUGXLCJ-OAHLLOKOSA-N 0.000 description 1
- 230000009758 senescence Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000014639 sexual reproduction Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 235000003579 silvertop stringybark Nutrition 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 230000000392 somatic effect Effects 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000007447 staining method Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 230000004960 subcellular localization Effects 0.000 description 1
- YROXIXLRRCOBKF-UHFFFAOYSA-N sulfonylurea Chemical compound OC(=N)N=S(=O)=O YROXIXLRRCOBKF-UHFFFAOYSA-N 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 235000005221 swamp mahogany Nutrition 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- ABZLKHKQJHEPAX-UHFFFAOYSA-N tetramethylrhodamine Chemical compound C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C([O-])=O ABZLKHKQJHEPAX-UHFFFAOYSA-N 0.000 description 1
- ZRKFYGHZFMAOKI-QMGMOQQFSA-N tgfbeta Chemical compound C([C@H](NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CCSC)C(C)C)[C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O)C1=CC=C(O)C=C1 ZRKFYGHZFMAOKI-QMGMOQQFSA-N 0.000 description 1
- 238000005382 thermal cycling Methods 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 125000000341 threoninyl group Chemical group [H]OC([H])(C([H])([H])[H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- 230000025366 tissue development Effects 0.000 description 1
- 229950003937 tolonium Drugs 0.000 description 1
- HNONEKILPDHFOL-UHFFFAOYSA-M tolonium chloride Chemical compound [Cl-].C1=C(C)C(N)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 HNONEKILPDHFOL-UHFFFAOYSA-M 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 230000005758 transcription activity Effects 0.000 description 1
- 108091006106 transcriptional activators Proteins 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- RTKIYFITIVXBLE-QEQCGCAPSA-N trichostatin A Chemical compound ONC(=O)/C=C/C(/C)=C/[C@@H](C)C(=O)C1=CC=C(N(C)C)C=C1 RTKIYFITIVXBLE-QEQCGCAPSA-N 0.000 description 1
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
- 230000014848 ubiquitin-dependent protein catabolic process Effects 0.000 description 1
- 238000010798 ubiquitination Methods 0.000 description 1
- 230000034512 ubiquitination Effects 0.000 description 1
- 230000003827 upregulation Effects 0.000 description 1
- 235000003456 urn gum Nutrition 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 108700026220 vif Genes Proteins 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
- 229940011671 vitamin b6 Drugs 0.000 description 1
- 238000003260 vortexing Methods 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
- YQCXUOPQUQUHAN-RLPXVTJZSA-L zinc (2R)-2-amino-3-hydroxy-3-oxopropane-1-thiolate (2S)-2-amino-3-imidazol-1-id-4-ylpropanoic acid hydron Chemical compound [H+].[H+].[Zn++].N[C@@H](C[S-])C(O)=O.N[C@@H](C[S-])C(O)=O.N[C@@H](C[S-])C(O)=O.N[C@@H](Cc1c[n-]cn1)C(O)=O YQCXUOPQUQUHAN-RLPXVTJZSA-L 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4738—Cell cycle regulated proteins, e.g. cyclin, CDC, INK-CCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Gastroenterology & Hepatology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Toxicology (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Paper (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2012202108A AU2012202108A1 (en) | 2003-12-30 | 2012-04-12 | Cell cycle genes and related methods of using |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US53303603P | 2003-12-30 | 2003-12-30 | |
| US60/533,036 | 2003-12-30 | ||
| PCT/US2004/043804 WO2005065339A2 (en) | 2003-12-30 | 2004-12-30 | Cell cycle genes and related methods of using |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2012202108A Division AU2012202108A1 (en) | 2003-12-30 | 2012-04-12 | Cell cycle genes and related methods of using |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2004311384A1 AU2004311384A1 (en) | 2005-07-21 |
| AU2004311384B2 true AU2004311384B2 (en) | 2012-01-12 |
Family
ID=34748844
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2004311384A Ceased AU2004311384B2 (en) | 2003-12-30 | 2004-12-30 | Cell cycle genes and related methods of using |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US7598084B2 (enExample) |
| EP (1) | EP1711592A4 (enExample) |
| JP (2) | JP2007523636A (enExample) |
| CN (1) | CN1954071A (enExample) |
| AR (1) | AR047574A1 (enExample) |
| AU (1) | AU2004311384B2 (enExample) |
| BR (1) | BRPI0418229A (enExample) |
| NZ (1) | NZ548845A (enExample) |
| WO (1) | WO2005065339A2 (enExample) |
| ZA (1) | ZA200606198B (enExample) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110047644A1 (en) * | 1999-03-11 | 2011-02-24 | Marion Wood | Compositions and methods for the modification of gene transcription |
| AR047574A1 (es) | 2003-12-30 | 2006-01-25 | Arborgen Llc 2 Genesis Res 1 | Genes del ciclo celular y metodos de uso relacionados |
| US8088975B2 (en) * | 2006-10-27 | 2012-01-03 | Ceres, Inc. | Phenylpropanoid related regulatory protein-regulatory region associations |
| US9758790B2 (en) | 2004-12-08 | 2017-09-12 | Ceres, Inc. | Modulating the level of components within plants |
| EP1882392A4 (en) * | 2005-05-10 | 2009-07-01 | Monsanto Technology Llc | GENES AND THEIR USES FOR PLANT IMPROVEMENTS |
| US8362322B2 (en) | 2006-10-27 | 2013-01-29 | Ceres, Inc. | Modulating lignin in plants |
| CN101652480A (zh) * | 2007-03-23 | 2010-02-17 | 巴斯福植物科学有限公司 | 具有提高的胁迫耐受性和产量的转基因植物 |
| WO2008120659A1 (ja) * | 2007-03-30 | 2008-10-09 | Oji Paper Co., Ltd. | 遺伝子発現情報を用いた植物の形質を判定もしくは予測する方法 |
| CN101960012B (zh) * | 2007-12-28 | 2014-03-12 | 瑞典树木科技公司 | 具有改善生长特征的木本植物以及使用转录因子制备它们的方法 |
| CN102888409B (zh) * | 2012-07-31 | 2015-04-22 | 普罗米绿色能源(深圳)有限公司 | 桉树pgef10基因及其植物表达载体、宿主细胞和应用 |
| CN102888411B (zh) * | 2012-07-31 | 2014-07-02 | 普罗米绿色能源(深圳)有限公司 | 桉树pgef13基因及其植物表达载体、宿主细胞和应用 |
| CN102888410B (zh) * | 2012-07-31 | 2015-04-22 | 普罗米绿色能源(深圳)有限公司 | 桉树pgef17基因及其植物表达载体、宿主细胞和应用 |
| AU2016331021B2 (en) * | 2015-10-02 | 2022-10-27 | Keygene N.V. | Method for the production of haploid and subsequent doubled haploid plants |
| CN110317817B (zh) * | 2019-07-16 | 2021-03-19 | 北京林业大学 | Ylb9基因序列、应用及调控植物木质素合成的方法 |
| CN110885813B (zh) * | 2019-12-17 | 2021-07-27 | 中国农业大学 | 水稻组蛋白去乙酰化酶基因hda710在延迟叶片衰老中的应用 |
| CN112048010B (zh) * | 2020-08-20 | 2022-06-17 | 华南农业大学 | 水稻rip2蛋白在调控植物叶夹角中的应用 |
| CN112458189A (zh) * | 2020-10-24 | 2021-03-09 | 宁波国际旅行卫生保健中心(宁波海关口岸门诊部) | 一种用于单增李斯特菌荧光raa检测的引物和探针序列及其应用 |
| CN113881699B (zh) * | 2021-11-05 | 2024-01-09 | 河南大学 | Mac3a和mac3b在植物器官大小调控中的应用 |
| CN116218899B (zh) * | 2023-02-14 | 2024-05-31 | 中国科学院遗传与发育生物学研究所 | 水稻特异调控粒宽基因slg2及其应用 |
| CN119776408B (zh) * | 2024-12-10 | 2025-11-25 | 河南农业大学 | 小麦TaCKS1蛋白、编码基因及其突变体序列在调控小麦籽粒发育中的应用 |
| CN121137057A (zh) * | 2025-11-13 | 2025-12-16 | 河南大学三亚研究院 | CycC1;1蛋白或其编码基因在调控植物叶片衰老中的应用和方法 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0154204B1 (en) | 1984-03-06 | 1994-01-12 | Mgi Pharma, Inc. | Herbicide resistance in plants |
| GB8626879D0 (en) | 1986-11-11 | 1986-12-10 | Ici Plc | Dna |
| JP3488465B2 (ja) | 1993-10-28 | 2004-01-19 | ヒューストン・アドバンスド・リサーチ・センター | 結合反応を別々に検出する微細加工フロースルー多孔性装置 |
| US5547861A (en) | 1994-04-18 | 1996-08-20 | Becton, Dickinson And Company | Detection of nucleic acid amplification |
| US6252139B1 (en) * | 1996-07-18 | 2001-06-26 | The Salk Institute For Biological Studies | Method of increasing growth and yield in plants |
| DE69822206T2 (de) | 1997-12-19 | 2005-02-17 | Affymetrix, Inc., Santa Clara | Erkenntnisse der genomforschung für die suche nach neuartigen wirkstoffen |
| CA2266295A1 (en) | 1999-02-26 | 2000-09-19 | Heberle-Bors, Erwin | Method of modifying plant metabolism and development |
| CA2367476A1 (en) * | 1999-03-19 | 2000-09-28 | Cropdesign N.V. | Method for enhancing and/or improving plant growth and/or yield or modifying plant architecture |
| AU4459100A (en) * | 1999-04-22 | 2000-11-10 | Pioneer Hi-Bred International, Inc. | Cell cycle genes and methods of use |
| CA2379850A1 (en) * | 1999-07-19 | 2001-01-25 | Japan Science And Technology Corporation | Environmental stress tolerant gene |
| PT1309726E (pt) | 2000-03-30 | 2010-03-08 | Whitehead Biomedical Inst | Mediadores de interferência por rna específicos de sequência de rna |
| US6606568B2 (en) | 2000-06-28 | 2003-08-12 | Midwest Research Institute | Method for predicting dry mechanical properties from wet wood and standing trees |
| US6525319B2 (en) | 2000-12-15 | 2003-02-25 | Midwest Research Institute | Use of a region of the visible and near infrared spectrum to predict mechanical properties of wet wood and standing trees |
| AU1456602A (en) | 2000-10-10 | 2002-04-22 | Westvaco Corp | Enhanced selection of genetically modified pine embryogenic tissue |
| AR047574A1 (es) | 2003-12-30 | 2006-01-25 | Arborgen Llc 2 Genesis Res 1 | Genes del ciclo celular y metodos de uso relacionados |
-
2004
- 2004-12-29 AR ARP040104950A patent/AR047574A1/es unknown
- 2004-12-30 US US11/024,959 patent/US7598084B2/en not_active Expired - Fee Related
- 2004-12-30 AU AU2004311384A patent/AU2004311384B2/en not_active Ceased
- 2004-12-30 NZ NZ548845A patent/NZ548845A/en not_active IP Right Cessation
- 2004-12-30 CN CNA200480042006XA patent/CN1954071A/zh active Pending
- 2004-12-30 JP JP2006547528A patent/JP2007523636A/ja active Pending
- 2004-12-30 BR BRPI0418229-4A patent/BRPI0418229A/pt not_active Application Discontinuation
- 2004-12-30 EP EP04815805A patent/EP1711592A4/en not_active Withdrawn
- 2004-12-30 WO PCT/US2004/043804 patent/WO2005065339A2/en not_active Ceased
-
2006
- 2006-07-26 ZA ZA200606198A patent/ZA200606198B/xx unknown
-
2009
- 2009-09-09 US US12/555,853 patent/US20100122382A1/en not_active Abandoned
-
2010
- 2010-11-10 JP JP2010251599A patent/JP2011097941A/ja active Pending
Non-Patent Citations (1)
| Title |
|---|
| Database EMBL, Accession No. CF477422, 08 September 2003 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2005065339A2 (en) | 2005-07-21 |
| ZA200606198B (en) | 2008-07-30 |
| JP2011097941A (ja) | 2011-05-19 |
| EP1711592A2 (en) | 2006-10-18 |
| EP1711592A4 (en) | 2007-08-22 |
| US7598084B2 (en) | 2009-10-06 |
| JP2007523636A (ja) | 2007-08-23 |
| BRPI0418229A (pt) | 2007-04-27 |
| AR047574A1 (es) | 2006-01-25 |
| AU2004311384A1 (en) | 2005-07-21 |
| US20100122382A1 (en) | 2010-05-13 |
| NZ548845A (en) | 2010-03-26 |
| WO2005065339A3 (en) | 2006-09-21 |
| CN1954071A (zh) | 2007-04-25 |
| US20060010516A1 (en) | 2006-01-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20100122382A1 (en) | Cell Cycle Genes and Related Methods | |
| JP4909072B2 (ja) | 転写因子 | |
| US7371927B2 (en) | Methods for modulating plant growth and biomass | |
| US8053237B2 (en) | Plant cell signaling genes | |
| Chai et al. | The NAC transcription factor OsSWN1 regulates secondary cell wall development in Oryza sativa | |
| JP5155875B2 (ja) | 木質部および細胞壁の遺伝子マイクロアレイ | |
| AU2011201695B2 (en) | Compositions and methods for regulating polysaccharides of a plant cell | |
| AU2012202108A1 (en) | Cell cycle genes and related methods of using | |
| ZA200601498B (en) | Plant cell cycle genes and methods of use |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PC1 | Assignment before grant (sect. 113) |
Owner name: ABORGEN, LLC Free format text: FORMER APPLICANT(S): ABORGEN, LLC; GENESIS RESEARCH AND DEVELOPMENT CORPORATION LIMITED |
|
| DA3 | Amendments made section 104 |
Free format text: THE NATURE OF THE AMENDMENT IS: AMEND THE NAME OF THE INVENTOR TO READ GRIGOR, MURRAY ROBERT; MAGUSIN, ANDREAS; CONNETT, MARIE B.; EMERSON, SARAH JANE; HIGGINS, COLLEEN M.; LUND, STEVEN TROY; KODRZYCKI, ROBERT JOHN AND FORSTER, RICHARD L.S. |
|
| FGA | Letters patent sealed or granted (standard patent) | ||
| MK14 | Patent ceased section 143(a) (annual fees not paid) or expired |