AU2004286261B2 - Method of designing siRNAs for gene silencing - Google Patents
Method of designing siRNAs for gene silencing Download PDFInfo
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- AU2004286261B2 AU2004286261B2 AU2004286261A AU2004286261A AU2004286261B2 AU 2004286261 B2 AU2004286261 B2 AU 2004286261B2 AU 2004286261 A AU2004286261 A AU 2004286261A AU 2004286261 A AU2004286261 A AU 2004286261A AU 2004286261 B2 AU2004286261 B2 AU 2004286261B2
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
- G16B20/20—Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
- G16B20/30—Detection of binding sites or motifs
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
- G16B20/50—Mutagenesis
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
- G16B30/10—Sequence alignment; Homology search
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering N.A.
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2320/00—Applications; Uses
- C12N2320/10—Applications; Uses in screening processes
- C12N2320/11—Applications; Uses in screening processes for the determination of target sites, i.e. of active nucleic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2330/00—Production
- C12N2330/30—Production chemically synthesised
- C12N2330/31—Libraries, arrays
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Evolutionary Biology (AREA)
- Molecular Biology (AREA)
- Medical Informatics (AREA)
- Analytical Chemistry (AREA)
- Theoretical Computer Science (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US51518003P | 2003-10-27 | 2003-10-27 | |
US60/515,180 | 2003-10-27 | ||
US57231404P | 2004-05-17 | 2004-05-17 | |
US60/572,314 | 2004-05-17 | ||
PCT/US2004/035636 WO2005042708A2 (en) | 2003-10-27 | 2004-10-27 | METHOD OF DESIGNING siRNAS FOR GENE SILENCING |
Publications (2)
Publication Number | Publication Date |
---|---|
AU2004286261A1 AU2004286261A1 (en) | 2005-05-12 |
AU2004286261B2 true AU2004286261B2 (en) | 2010-06-24 |
Family
ID=34555961
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU2004286261A Active AU2004286261B2 (en) | 2003-10-27 | 2004-10-27 | Method of designing siRNAs for gene silencing |
Country Status (8)
Country | Link |
---|---|
US (2) | US7962316B2 (ja) |
EP (1) | EP1692262B1 (ja) |
JP (1) | JP4790619B2 (ja) |
CN (1) | CN1926551B (ja) |
AU (1) | AU2004286261B2 (ja) |
CA (1) | CA2543954C (ja) |
ES (1) | ES2687645T3 (ja) |
WO (1) | WO2005042708A2 (ja) |
Families Citing this family (37)
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US7199107B2 (en) * | 2002-05-23 | 2007-04-03 | Isis Pharmaceuticals, Inc. | Antisense modulation of kinesin-like 1 expression |
US8609830B2 (en) | 2003-05-16 | 2013-12-17 | Merck Sharp & Dohme Corp. | Methods and compositions for RNA interference |
JP4747245B2 (ja) * | 2003-12-31 | 2011-08-17 | 謙造 廣瀬 | RNAiライブラリーの酵素的構築方法 |
CN1977052B (zh) * | 2004-03-23 | 2012-09-05 | 肿瘤疗法科学股份有限公司 | 与kif11基因相关的双-链分子及载体 |
EP1931789B1 (en) | 2005-09-20 | 2016-05-04 | BASF Plant Science GmbH | Methods for controlling gene expression using ta-siran |
WO2007147067A2 (en) | 2006-06-14 | 2007-12-21 | Rosetta Inpharmatics Llc | Methods and compositions for regulating cell cycle progression |
PL2119447T3 (pl) * | 2007-02-16 | 2013-08-30 | Oncotherapy Science Inc | Terapia szczepionkowa przeciwko neowaskularyzacji naczyniówkowej |
US8158677B2 (en) | 2007-06-01 | 2012-04-17 | The Trustees Of Princeton University | Treatment of viral infections by modulation of host cell metabolic pathways |
WO2009042115A2 (en) * | 2007-09-24 | 2009-04-02 | Rosetta Inpharmatics Llc | Methods of designing short hairpin rnas (shrnas) for gene silencing |
US8685937B2 (en) * | 2008-08-09 | 2014-04-01 | University Of Iowa Research Foundation | Nucleic acid aptamers |
US20120072123A1 (en) * | 2009-05-29 | 2012-03-22 | Merck & Co., Inc. | Methods of Predicting The Probability of Modulation of Transcript Levels By RNAI Compounds |
TW201109029A (en) | 2009-06-11 | 2011-03-16 | Oncotherapy Science Inc | Vaccine therapy for choroidal neovascularization |
EP2329854A3 (de) * | 2009-12-04 | 2014-02-19 | Biotronik VI Patent AG | Implantatbeschichtung mit Nukleinsäuren |
WO2013043878A2 (en) * | 2011-09-20 | 2013-03-28 | The George Washington University | Alternative splicing variants of genes associated with prostate cancer risk and survival |
CN102643815A (zh) * | 2012-03-12 | 2012-08-22 | 中国水产科学研究院东海水产研究所 | 一种抗RNA病毒siRNA分子的设计方法 |
EP2970976A4 (en) * | 2013-03-15 | 2016-11-02 | Techulon Inc | ANTISENSE MOLECULES FOR THE TREATMENT OF STAPHYLOCOCCUS AUREUS INFECTIONS |
US10137143B1 (en) * | 2014-07-30 | 2018-11-27 | The Administrators Of The Tulane Educational Fund | Preventing tumor development and metastasis |
SG11201703419UA (en) | 2014-11-14 | 2017-05-30 | Voyager Therapeutics Inc | Modulatory polynucleotides |
CN107109407A (zh) | 2014-11-14 | 2017-08-29 | 沃雅戈治疗公司 | 治疗肌萎缩性侧索硬化(als)的组合物和方法 |
CN107090596B (zh) * | 2016-02-18 | 2020-08-28 | 中国科学院分子细胞科学卓越创新中心 | 建立克服基因功能冗余的全基因组功能缺失筛选方法 |
CA3024449A1 (en) | 2016-05-18 | 2017-11-23 | Voyager Therapeutics, Inc. | Compositions and methods of treating huntington's disease |
CN110214187B (zh) | 2016-05-18 | 2024-01-30 | 沃雅戈治疗公司 | 调节性多核苷酸 |
CN108182346B (zh) * | 2016-12-08 | 2021-07-30 | 杭州康万达医药科技有限公司 | 预测siRNA针对某类细胞的毒性的机器学习模型的建立方法及其应用 |
WO2018111978A1 (en) | 2016-12-14 | 2018-06-21 | Janssen Biotech, Inc. | Cd137 binding fibronectin type iii domains |
US10597438B2 (en) | 2016-12-14 | 2020-03-24 | Janssen Biotech, Inc. | PD-L1 binding fibronectin type III domains |
JP7104703B2 (ja) | 2016-12-14 | 2022-07-21 | ヤンセン バイオテツク,インコーポレーテツド | Cd8a結合フィブロネクチンiii型ドメイン |
SG11201909868YA (en) | 2017-05-05 | 2019-11-28 | Voyager Therapeutics Inc | Compositions and methods of treating huntington's disease |
EP3618839A4 (en) | 2017-05-05 | 2021-06-09 | Voyager Therapeutics, Inc. | COMPOSITIONS AND TREATMENT METHODS FOR AMYOTROPHIC LATERAL SCLEROSIS (ALS) |
AU2018352236A1 (en) | 2017-10-16 | 2020-04-23 | The Curators Of The University Of Missouri | Treatment of amyotrophic lateral sclerosis (ALS) |
WO2019079242A1 (en) | 2017-10-16 | 2019-04-25 | Voyager Therapeutics, Inc. | TREATMENT OF AMYOTROPHIC LATERAL SCLEROSIS (ALS) |
TWI709188B (zh) | 2018-09-27 | 2020-11-01 | 財團法人工業技術研究院 | 基於機率融合的分類器、分類方法及分類系統 |
CN110295171B (zh) * | 2019-06-26 | 2022-07-22 | 中山大学附属第六医院 | 用于抑制NPC1基因表达的siRNA的应用 |
US11628222B2 (en) | 2019-10-14 | 2023-04-18 | Aro Biotherapeutics Company | CD71 binding fibronectin type III domains |
US11781138B2 (en) | 2019-10-14 | 2023-10-10 | Aro Biotherapeutics Company | FN3 domain-siRNA conjugates and uses thereof |
WO2023122805A1 (en) | 2021-12-20 | 2023-06-29 | Vestaron Corporation | Sorbitol driven selection pressure method |
CN116798513B (zh) * | 2023-02-21 | 2023-12-15 | 苏州赛赋新药技术服务有限责任公司 | 筛选siRNA序列以降低脱靶效应的方法及系统 |
CN116825199A (zh) * | 2023-02-21 | 2023-09-29 | 王全军 | 筛选siRNA序列以降低脱靶效应的方法及系统 |
Citations (2)
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WO2003065281A1 (en) * | 2002-01-29 | 2003-08-07 | Health Research, Inc. | Statistical algorithms for folding and target accessibility prediction and design of nucleic acids |
US20030175950A1 (en) * | 2001-05-29 | 2003-09-18 | Mcswiggen James A. | RNA interference mediated inhibition of HIV gene expression using short interfering RNA |
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US4980286A (en) | 1985-07-05 | 1990-12-25 | Whitehead Institute For Biomedical Research | In vivo introduction and expression of foreign genetic material in epithelial cells |
CA2092323A1 (en) | 1990-10-01 | 1992-04-02 | George Y. Wu | Targeting viruses and cells for selective internalization by cells |
WO1992020316A2 (en) | 1991-05-14 | 1992-11-26 | University Of Connecticut | Targeted delivery of genes encoding immunogenic proteins |
EP0587738B1 (en) | 1991-06-05 | 2000-08-23 | University Of Connecticut | Targeted delivery of genes encoding secretory proteins |
WO1993014188A1 (en) | 1992-01-17 | 1993-07-22 | The Regents Of The University Of Michigan | Targeted virus |
AU3940293A (en) | 1992-04-03 | 1993-11-08 | Alexander T. YOUNG | Gene therapy using targeted viral vectors |
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US20030143597A1 (en) * | 2000-12-28 | 2003-07-31 | Finney Robert E. | Methods for making polynucleotide libraries, polynucleotide arrays, and cell libraries for high-throughput genomics analysis |
KR20040022449A (ko) | 2001-07-12 | 2004-03-12 | 유니버시티 오브 매사추세츠 | 유전자 불활성화를 매개하는 소형 간섭 rna의 생체내제조 |
JP2005525790A (ja) | 2001-11-19 | 2005-09-02 | プロテオロジックス,インク. | 潜在的薬物ターゲットを同定及びバリデートする方法 |
WO2003064621A2 (en) * | 2002-02-01 | 2003-08-07 | Ambion, Inc. | HIGH POTENCY siRNAS FOR REDUCING THE EXPRESSION OF TARGET GENES |
WO2004005547A2 (en) * | 2002-07-04 | 2004-01-15 | Imperial College Innovations Limited | Method for identifying hypersensitive site consensus sequences |
CN1440814A (zh) * | 2002-11-28 | 2003-09-10 | 张辰宇 | 特异性爱滋病病毒rna降解剂及其制备方法 |
US8005620B2 (en) * | 2003-08-01 | 2011-08-23 | Dna Twopointo Inc. | Systems and methods for biopolymer engineering |
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2004
- 2004-10-27 CA CA2543954A patent/CA2543954C/en active Active
- 2004-10-27 WO PCT/US2004/035636 patent/WO2005042708A2/en active Application Filing
- 2004-10-27 AU AU2004286261A patent/AU2004286261B2/en active Active
- 2004-10-27 ES ES04810056.4T patent/ES2687645T3/es active Active
- 2004-10-27 JP JP2006538215A patent/JP4790619B2/ja active Active
- 2004-10-27 CN CN2004800392267A patent/CN1926551B/zh active Active
- 2004-10-27 US US10/577,696 patent/US7962316B2/en active Active
- 2004-10-27 EP EP04810056.4A patent/EP1692262B1/en active Active
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2011
- 2011-05-04 US US13/100,836 patent/US8457902B2/en active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030175950A1 (en) * | 2001-05-29 | 2003-09-18 | Mcswiggen James A. | RNA interference mediated inhibition of HIV gene expression using short interfering RNA |
WO2003065281A1 (en) * | 2002-01-29 | 2003-08-07 | Health Research, Inc. | Statistical algorithms for folding and target accessibility prediction and design of nucleic acids |
Also Published As
Publication number | Publication date |
---|---|
CA2543954C (en) | 2016-11-08 |
US20110250591A1 (en) | 2011-10-13 |
ES2687645T3 (es) | 2018-10-26 |
CA2543954A1 (en) | 2005-05-12 |
US20080234941A1 (en) | 2008-09-25 |
AU2004286261A1 (en) | 2005-05-12 |
EP1692262A4 (en) | 2008-07-09 |
US8457902B2 (en) | 2013-06-04 |
CN1926551A (zh) | 2007-03-07 |
JP4790619B2 (ja) | 2011-10-12 |
EP1692262A2 (en) | 2006-08-23 |
WO2005042708A3 (en) | 2006-10-26 |
EP1692262B1 (en) | 2018-08-15 |
WO2005042708A2 (en) | 2005-05-12 |
US7962316B2 (en) | 2011-06-14 |
JP2007512808A (ja) | 2007-05-24 |
CN1926551B (zh) | 2010-06-16 |
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Owner name: MERCK SHARP & DOHME LLC Free format text: FORMER NAME(S): MERCK SHARP & DOHME CORP. |