AU2002257243A1 - Rapidly cleavable sumo fusion protein expression system for difficult to express proteins - Google Patents

Rapidly cleavable sumo fusion protein expression system for difficult to express proteins

Info

Publication number
AU2002257243A1
AU2002257243A1 AU2002257243A AU2002257243A AU2002257243A1 AU 2002257243 A1 AU2002257243 A1 AU 2002257243A1 AU 2002257243 A AU2002257243 A AU 2002257243A AU 2002257243 A AU2002257243 A AU 2002257243A AU 2002257243 A1 AU2002257243 A1 AU 2002257243A1
Authority
AU
Australia
Prior art keywords
fusion protein
amino acid
expression system
difficult
protein expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002257243A
Other languages
English (en)
Inventor
Christopher D. Lima
Elena Mossessova
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cornell Research Foundation Inc
Original Assignee
Cornell Research Foundation Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cornell Research Foundation Inc filed Critical Cornell Research Foundation Inc
Publication of AU2002257243A1 publication Critical patent/AU2002257243A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/23—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/43—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a FLAG-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2002257243A 2001-05-04 2002-05-06 Rapidly cleavable sumo fusion protein expression system for difficult to express proteins Abandoned AU2002257243A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US28865601P 2001-05-04 2001-05-04
US60/288,656 2001-05-04
US32908001P 2001-10-12 2001-10-12
US60/329,080 2001-10-12
PCT/US2002/014062 WO2002090495A2 (en) 2001-05-04 2002-05-06 Rapidly cleavable sumo fusion protein expression system for difficult to express proteins

Publications (1)

Publication Number Publication Date
AU2002257243A1 true AU2002257243A1 (en) 2002-11-18

Family

ID=26965151

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002257243A Abandoned AU2002257243A1 (en) 2001-05-04 2002-05-06 Rapidly cleavable sumo fusion protein expression system for difficult to express proteins

Country Status (8)

Country Link
US (3) US6872551B2 (https=)
EP (1) EP1392717B1 (https=)
JP (1) JP2004532033A (https=)
AT (1) ATE490968T1 (https=)
AU (1) AU2002257243A1 (https=)
CA (1) CA2445049A1 (https=)
DE (1) DE60238532D1 (https=)
WO (1) WO2002090495A2 (https=)

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US8535908B2 (en) * 2002-01-04 2013-09-17 Brookhaven Science Associates, Llc Facilitating protein solubility by use of peptide extensions
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US7220576B2 (en) * 2002-01-07 2007-05-22 Lifesensors, Inc. Methods and compositions for protein expression and purification
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AU2004254600A1 (en) * 2003-06-26 2005-01-13 Lifesensors, Inc. Methods and compositions for enhanced protein expression and purification
WO2005014796A2 (en) 2003-08-08 2005-02-17 Invitrogen Corporation Methods and compositions for seamless cloning of nucleic acid molecules
US20090123468A1 (en) 2003-10-24 2009-05-14 Gencia Corporation Transducible polypeptides for modifying metabolism
US8133733B2 (en) 2003-10-24 2012-03-13 Gencia Corporation Nonviral vectors for delivering polynucleotides to target tissues
US8507277B2 (en) 2003-10-24 2013-08-13 Gencia Corporation Nonviral vectors for delivering polynucleotides
US8062891B2 (en) 2003-10-24 2011-11-22 Gencia Corporation Nonviral vectors for delivering polynucleotides to plants
US20090208478A1 (en) * 2003-10-24 2009-08-20 Gencia Corporation Transducible polypeptides for modifying metabolism
CA2543257C (en) 2003-10-24 2013-12-31 Gencia Corporation Methods and compositions for delivering polynucleotides
WO2005054438A2 (en) 2003-12-01 2005-06-16 Invitrogen Corporation Nucleic acid molecules containing recombination sites and methods of using the same
ES2306541B1 (es) * 2004-04-16 2009-09-28 Consejo Superior Investig. Cientificas Trasplante funcional de la maquinaria de sumoilacion a escherichia coli.
EP1791961B1 (en) * 2004-08-18 2011-06-08 The Governors of the University of Alberta Protein production method utilizing yebf
EP1836214A4 (en) * 2004-12-30 2009-01-21 Lifesensors Inc COMPOSITIONS, METHODS, AND KITS FOR IMPROVING PROTEIN EXPRESSION, SOLUBILITY, AND ISOLATION
US20100048480A1 (en) * 2006-11-22 2010-02-25 Bettina Bommarius Production of anti-microbial peptides
EP2100961A1 (en) * 2008-03-04 2009-09-16 Icon Genetics GmbH Method of protease production in plants
US8034910B2 (en) * 2008-05-06 2011-10-11 Academia Sinica, Taiwan SUMO fusion protein expression system for producing native proteins
TW200948959A (en) * 2008-05-06 2009-12-01 Academia Sinica Preparation of soluble capsid proteins of picornavirus using SUMO fusion technology
US8440449B2 (en) * 2008-09-30 2013-05-14 The United States Of America, As Represented By The Secretary Of Agriculture Transformed Saccharomyces cerevisiae engineered for xylose utilization
US8663951B2 (en) * 2009-01-09 2014-03-04 Academia Sinica Method of producing virus-like particles of picornavirus using a small-ubiquitin-related fusion protein expression system
CN102533712A (zh) * 2009-05-12 2012-07-04 中国科学院上海生命科学研究院 一种固相化sumo化系统及固相化去sumo化系统
CN101993495B (zh) * 2009-08-12 2013-07-24 上海近岸科技有限公司 一种蛋白质混合物及其制备方法
CN103184228A (zh) * 2011-12-29 2013-07-03 天津市国际生物医药联合研究院 用于可结晶成熟型SARS冠状病毒非结构蛋白12(sars-nsp12)的外源重组表达及纯化方法
WO2013103964A1 (en) 2012-01-06 2013-07-11 Gencia Corporation Methods and compositions for treating proteinopathies
WO2013188873A1 (en) 2012-06-15 2013-12-19 Gencia Corporation Compositions and methods for enhancing immune responses
WO2013188874A1 (en) 2012-06-15 2013-12-19 Gencia Corporation Methods of mitigating side effects of radiation exposure and chemotherapy
DK2904101T3 (da) 2012-10-03 2019-08-12 Agrivida Inc Intein-modificerede proteaser, fremstilling deraf og industrielle anvendelser
KR101911438B1 (ko) 2012-10-31 2018-10-24 삼성전자주식회사 이중 특이 항원 결합 단백질 복합체 및 이중 특이 항체의 제조 방법
US9707244B2 (en) 2013-03-15 2017-07-18 Gencia Corporation Compositions and methods for treating conditions that affect epidermis
US9970945B2 (en) 2013-06-07 2018-05-15 Georgia State University Research Foundtion, Inc. Compositions, methods, and assays using proteo-liposome-transfected oocytes for analyzing the structure, function, and activity of membrane proteins
US9447157B2 (en) 2013-09-13 2016-09-20 Augusta University Research Institute, Inc. Nitration shielding peptides and methods of use thereof
WO2015049230A1 (en) 2013-10-01 2015-04-09 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. An orthogonal set of tag-cleaving proteases for purification of proteins and stoichiometric protein complexes
US10378003B2 (en) 2014-11-10 2019-08-13 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Tag removal from proteins expressed in pro- and eukaryotic hosts
JP6997704B2 (ja) 2015-10-14 2022-02-04 トランスレイト バイオ， インコーポレイテッド 生産性向上のためのｒｎａ関連酵素の修飾
TWI591177B (zh) * 2016-10-27 2017-07-11 中化合成生技股份有限公司 一種胰高血糖素胜肽－２（ｇｌｐ－２）類似物的製備方法
US10913777B2 (en) 2017-05-22 2021-02-09 The Regents Of The University Of California In vitro assembly of bacterial microcompartments
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KR20190135393A (ko) * 2018-05-28 2019-12-06 주식회사 원진바이오테크놀로지 폴리유비퀴틴 스캐폴드에 결합된 생체분자들의 선형 멀티머 중합체 및 이의 용도
CN109517074A (zh) * 2018-12-04 2019-03-26 湖北大学 一种融合标签促进Kuma030蛋白酶可溶性表达及非亲和层析快速纯化的应用
CN109628479A (zh) * 2018-12-25 2019-04-16 天津农学院 一种胰高血糖素样肽-1的重组表达方法
US20220073908A1 (en) * 2019-01-04 2022-03-10 Repertoire Immune Medicines, Inc. Methods of producing high diversity peptide libraries and promoting protein folding
EP4100038A4 (en) 2020-02-03 2024-02-28 Premas Biotech Private Limited RECOMBINANT EXPRESSION PLATFORM, CONSTRUCTS AND METHODS FOR EXPRESSING HARD-TO-EXPRESS PROTEINS (DTE-PS)
CN113025675B (zh) * 2021-05-21 2021-08-20 凯莱英医药集团（天津）股份有限公司 多肽的制备方法
US20250161429A1 (en) 2022-02-14 2025-05-22 University Of Georgia Research Foundation, Inc. Pan-pneumovirus vaccine compositions and methods of use thereof
CN114426586A (zh) * 2022-02-21 2022-05-03 中国农业大学 一种胞外分泌表达Ulp1蛋白酶的大肠杆菌基因工程菌株及其应用
CN114539425B (zh) * 2022-02-25 2023-04-28 湖南中晟全肽生化有限公司 一种提高线性多肽生物表达的方法

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DE60238532D1 (de) * 2001-05-04 2011-01-20 Cornell Res Foundation Inc Schnell spaltbares sumofusionsprotein-expressionssystem für schwer zu exprimierende proteine
CA2472477C (en) * 2002-01-07 2012-03-20 Lifesensors, Inc. Methods and compositions for protein expression and purification

Also Published As

Publication number Publication date
WO2002090495A8 (en) 2003-07-03
EP1392717B1 (en) 2010-12-08
US7910364B2 (en) 2011-03-22
US7498165B2 (en) 2009-03-03
US20090317868A1 (en) 2009-12-24
CA2445049A1 (en) 2002-11-14
WO2002090495A2 (en) 2002-11-14
US6872551B2 (en) 2005-03-29
US20050069988A1 (en) 2005-03-31
EP1392717A4 (en) 2005-06-08
EP1392717A1 (en) 2004-03-03
ATE490968T1 (de) 2010-12-15
JP2004532033A (ja) 2004-10-21
DE60238532D1 (de) 2011-01-20
US20030086918A1 (en) 2003-05-08

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Legal Events

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MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase