ATE121454T1 - Ubiquitinspezifische protease. - Google Patents

Ubiquitinspezifische protease.

Info

Publication number
ATE121454T1
ATE121454T1 AT91910453T AT91910453T ATE121454T1 AT E121454 T1 ATE121454 T1 AT E121454T1 AT 91910453 T AT91910453 T AT 91910453T AT 91910453 T AT91910453 T AT 91910453T AT E121454 T1 ATE121454 T1 AT E121454T1
Authority
AT
Austria
Prior art keywords
ubiquitin
peptide
protease
protein
moiety
Prior art date
Application number
AT91910453T
Other languages
English (en)
Inventor
Alexander J One Lon Varshavsky
John W Tobias
Original Assignee
Massachusetts Inst Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Massachusetts Inst Technology filed Critical Massachusetts Inst Technology
Application granted granted Critical
Publication of ATE121454T1 publication Critical patent/ATE121454T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Detergent Compositions (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AT91910453T 1990-05-09 1991-05-08 Ubiquitinspezifische protease. ATE121454T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US52108990A 1990-05-09 1990-05-09
US57395890A 1990-08-28 1990-08-28

Publications (1)

Publication Number Publication Date
ATE121454T1 true ATE121454T1 (de) 1995-05-15

Family

ID=27060368

Family Applications (1)

Application Number Title Priority Date Filing Date
AT91910453T ATE121454T1 (de) 1990-05-09 1991-05-08 Ubiquitinspezifische protease.

Country Status (9)

Country Link
US (1) US5391490A (de)
EP (1) EP0531404B1 (de)
JP (1) JP3041644B2 (de)
AT (1) ATE121454T1 (de)
CA (1) CA2079229C (de)
DE (1) DE69109109T2 (de)
DK (1) DK0531404T3 (de)
ES (1) ES2071315T3 (de)
WO (1) WO1991017245A1 (de)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5212058A (en) * 1990-05-09 1993-05-18 Massachusetts Institute Of Technology Nucleic acid encoding ubiquitin-specific proteases
WO1995018974A2 (en) * 1994-01-04 1995-07-13 Mitotix, Inc. Ubiquitin conjugating enzymes
US5981699A (en) * 1994-01-04 1999-11-09 Mitotix, Inc. Human ubiquitin conjugating enzyme
US5744343A (en) * 1994-01-04 1998-04-28 Mitotix, Inc. Ubiquitin conjugating enzymes
AU7357896A (en) 1995-08-09 1997-03-05 Dana-Farber Cancer Institute Deubiquitinating enzymes that regulate cell growth
US5770720A (en) * 1995-08-30 1998-06-23 Barnes-Jewish Hospital Ubiquitin conjugating enzymes having transcriptional repressor activity
US6001619A (en) * 1995-10-04 1999-12-14 Cold Spring Harbor Laboratory Ubiquitin ligases, and uses related thereto
US5861483A (en) * 1996-04-03 1999-01-19 Pro-Neuron, Inc. Inhibitor of stem cell proliferation and uses thereof
GB9618960D0 (en) 1996-09-11 1996-10-23 Medical Science Sys Inc Proteases
US6747128B2 (en) 1997-08-20 2004-06-08 Gpc Biotech, Inc. Components of ubiquitin ligase complexes, and uses related thereto
US5932422A (en) * 1997-11-14 1999-08-03 Millennium Pharmaceuticals, Inc. Modulation of drug resistance via ubiquitin carboxy-terminal hydrolase
US7668658B2 (en) * 1999-10-13 2010-02-23 Sequenom, Inc. Methods for generating databases and databases for identifying polymorphic genetic markers
WO2001066763A2 (en) * 2000-03-07 2001-09-13 Millennium Pharmaceuticals, Inc. 23436, a human ubiquitin protease family member and uses thereof
US7070947B2 (en) * 2000-02-29 2006-07-04 Millennium Pharmaceuticals, Inc. Human protein kinase, phosphatase, and protease family members and uses thereof
CZ20024085A3 (cs) 2000-06-15 2003-05-14 Smithkline Beecham Corporation Způsob přípravy fyziologicky aktivního IL-18 polypeptidu
US20030104570A1 (en) * 2000-06-26 2003-06-05 Cabezon Silva Teresa Elisa Virginia Triple fusion proteins comprising ubiquitin fused between thioredoxin and a polypeptide of interest
US7122332B2 (en) * 2001-03-29 2006-10-17 Rigel Pharmaceuticals, Inc. Modulators of leukocyte activation, compositions and methods of use
US7820378B2 (en) * 2002-11-27 2010-10-26 Sequenom, Inc. Fragmentation-based methods and systems for sequence variation detection and discovery
CA2523490A1 (en) * 2003-04-25 2004-11-11 Sequenom, Inc. Fragmentation-based methods and systems for de novo sequencing
US9394565B2 (en) * 2003-09-05 2016-07-19 Agena Bioscience, Inc. Allele-specific sequence variation analysis
EP1727911B1 (de) 2004-03-26 2013-01-23 Sequenom, Inc. Basenspezifische spaltung methylierungsspezifischer amplifikationsprodukte in kombination mit massenanalyse
US7608394B2 (en) 2004-03-26 2009-10-27 Sequenom, Inc. Methods and compositions for phenotype identification based on nucleic acid methylation
US20060073501A1 (en) * 2004-09-10 2006-04-06 Van Den Boom Dirk J Methods for long-range sequence analysis of nucleic acids
EP1841862B1 (de) 2005-01-10 2013-03-06 Instytut Biotechnologii I Antybiotykow Ubp1-proteasemutante sowie ihre codierende sequenz, deren anwendungen und heterogenes proteinexpressionssystem
US8173792B2 (en) * 2007-02-09 2012-05-08 The Board Of Trustees Of The Leland Stanford Junior University Method for regulating protein function in cells using synthetic small molecules
US20090006002A1 (en) * 2007-04-13 2009-01-01 Sequenom, Inc. Comparative sequence analysis processes and systems
US8530636B2 (en) * 2008-05-07 2013-09-10 The Board Of Trustees Of The Leland Stanford Junior University Method for regulating protein function in cells in vivo using synthetic small molecules
JP2021514678A (ja) * 2018-03-06 2021-06-17 ペップバックス・インコーポレイテッド 核酸分子およびそれを使用する方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988002406A2 (en) * 1986-10-02 1988-04-07 Massachusetts Institute Of Technology Methods of regulating metabolic stability of proteins
US5108919A (en) * 1988-06-24 1992-04-28 Genentech, Inc. Dna sequences encoding yeast ubiquitin hydrolase

Also Published As

Publication number Publication date
JPH06500006A (ja) 1994-01-06
JP3041644B2 (ja) 2000-05-15
CA2079229A1 (en) 1991-11-10
DE69109109T2 (de) 1995-09-14
DK0531404T3 (da) 1995-09-18
ES2071315T3 (es) 1995-06-16
EP0531404B1 (de) 1995-04-19
US5391490A (en) 1995-02-21
EP0531404A1 (de) 1993-03-17
WO1991017245A1 (en) 1991-11-14
CA2079229C (en) 1999-09-14
DE69109109D1 (de) 1995-05-24

Similar Documents

Publication Publication Date Title
ATE121454T1 (de) Ubiquitinspezifische protease.
Inouye Intramolecular chaperone: the role of the pro-peptide in protein folding
DE3584260D1 (de) Die synthese eines proteins mittels eines identifizierungspeptids.
Ugai et al. Purification and characterization of the 26S proteasome complex catalyzing ATP-dependent breakdown of ubiquitin-ligated prot from rat liver
NZ232742A (en) Yeast heterologous protein production method, vectors, dna constructs and proteins so produced and aprotinin (1-58) so produced
ATE272109T1 (de) Rekombinante collagenase typ i aus clostridium histolyticum und ihre verwendung zur isolierung von zellen und zellverbänden
WO1994018331A3 (en) Fusion proteins including a cleavage site recognized by a plant virus protease
ZA8860B (en) Pancreatic secretory trypsin inhibitor and variants thereof produced by a recombinant host,process,expression vector and recombinant host therefor and pharmaceutical use thereof
KR940703860A (ko) 신규한 사람 쿠니즈형 프로테아제 저해제 및 그의 변이체(a novel human kunitz-type protease inhibitor and variants thereof)
EP0528686B8 (de) Verfahren zur Herstellung von Peptiden
EP0195680A3 (de) Proteinsynthese mit einem Identifizierungspeptid
ATE317443T1 (de) N-terminal verlängerte proteine exprimiert in hefe
IL90947A (en) Method for production of hiv protease in transformed cells, dna and vectors comprising said dna used in said production
KR940009329A (ko) 딕티오스텔륨 디펩티딜아미노펩티다아제
Ohkubo et al. Molecular cloning and immunohistochemical localization of rat dipeptidyl peptidase III
Sokolovsky et al. Primary structure of cytochrome c from the camel, Camelus dromedarius
Harris et al. Amino acid sequences in penicillo-pepsin: evidence for homology with porcine pepsin and chymosin.
ATE309263T1 (de) Reinigungsverfahren auf grundlage der bindung von enzymen and markierte peptide
WO2001047958A3 (en) Kunitz domain polypeptide zkun10
Pal et al. Crystallization of an enzyme‐inhibitor complex: Proteinase K with its protein inhibitor, PK13
TH17635A (th) อะมิโนเพพติเดสชนิดใหม่ กระบวนการเตรียมอะมิโนเพพติเดสและโปรตีนจากมัน
TH17635EX (th) อะมิโนเพพติเดสชนิดใหม่ กระบวนการเตรียมอะมิโนเพพติเดสและโปรตีนจากมัน
JO2429B1 (en) Ways to produce recombinant peptides of antigen - merger

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee