AU2002217839A1 - A method to measure the activation state of signaling pathways in cells - Google Patents

A method to measure the activation state of signaling pathways in cells

Info

Publication number
AU2002217839A1
AU2002217839A1 AU2002217839A AU1783902A AU2002217839A1 AU 2002217839 A1 AU2002217839 A1 AU 2002217839A1 AU 2002217839 A AU2002217839 A AU 2002217839A AU 1783902 A AU1783902 A AU 1783902A AU 2002217839 A1 AU2002217839 A1 AU 2002217839A1
Authority
AU
Australia
Prior art keywords
reporter
amount
cell
enzyme
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002217839A
Other languages
English (en)
Inventor
Nancy L. Allbritton
Christopher Sims
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California
Original Assignee
University of California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California filed Critical University of California
Publication of AU2002217839A1 publication Critical patent/AU2002217839A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Signal Processing (AREA)
  • Dispersion Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU2002217839A 2000-11-22 2001-11-21 A method to measure the activation state of signaling pathways in cells Abandoned AU2002217839A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US25286100P 2000-11-22 2000-11-22
US60/252,861 2000-11-22
PCT/US2001/043966 WO2002042732A2 (fr) 2000-11-22 2001-11-21 Procede de mesure de l'etat d'activation des voies de signalisation dans des cellules

Publications (1)

Publication Number Publication Date
AU2002217839A1 true AU2002217839A1 (en) 2002-06-03

Family

ID=22957857

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002217839A Abandoned AU2002217839A1 (en) 2000-11-22 2001-11-21 A method to measure the activation state of signaling pathways in cells

Country Status (6)

Country Link
EP (1) EP1336101B1 (fr)
JP (1) JP2004514885A (fr)
AT (1) ATE317546T1 (fr)
AU (1) AU2002217839A1 (fr)
DE (1) DE60117180T2 (fr)
WO (1) WO2002042732A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6335201B1 (en) 1998-03-06 2002-01-01 The Regents Of The University Of California Method and apparatus for detecting enzymatic activity using molecules that change electrophoretic mobility
US6740497B2 (en) * 1998-03-06 2004-05-25 The Regents Of The University Of California Method and apparatus for detecting cancerous cells using molecules that change electrophoretic mobility
US7183066B2 (en) * 2002-09-27 2007-02-27 Allergan, Inc. Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins
DE102005059536B4 (de) * 2005-12-13 2008-08-28 Siemens Ag Verfahren und Biochip zur Untersuchung einer biologischen Probe
US20100291584A1 (en) * 2008-02-01 2010-11-18 The Regents Of The University Of California Microfluidic imaging cytometry
CN101231262B (zh) * 2008-02-22 2011-09-14 东南大学 用于单细胞组学的方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5258289A (en) * 1990-09-05 1993-11-02 Davis Claude G Method for the selecting of genes encoding catalytic antibodies
US5156576B1 (en) * 1991-05-22 1994-08-30 Sauer Inc Compact integrated transaxle
US5958703A (en) * 1996-12-03 1999-09-28 Glaxo Group Limited Use of modified tethers in screening compound libraries
US6335201B1 (en) * 1998-03-06 2002-01-01 The Regents Of The University Of California Method and apparatus for detecting enzymatic activity using molecules that change electrophoretic mobility
US6156576A (en) * 1998-03-06 2000-12-05 The Regents Of The University Of California Fast controllable laser lysis of cells for analysis
EP1121593A2 (fr) * 1998-10-15 2001-08-08 BioImage A/S Procede ameliore destine a une extraction quantitative d'information se rapportant a une influence sur une reponse cellulaire
CA2362117C (fr) * 1999-02-26 2004-11-30 Cellomics, Inc. Systeme de criblage cellulaire
WO2001044269A2 (fr) * 1999-12-17 2001-06-21 Large Scale Proteomics Corporation Proteines cerebrales qui sont des marqueurs

Also Published As

Publication number Publication date
EP1336101A4 (fr) 2004-08-18
DE60117180D1 (de) 2006-04-20
WO2002042732A3 (fr) 2002-08-01
JP2004514885A (ja) 2004-05-20
EP1336101A2 (fr) 2003-08-20
ATE317546T1 (de) 2006-02-15
EP1336101B1 (fr) 2006-02-08
WO2002042732A2 (fr) 2002-05-30
DE60117180T2 (de) 2006-08-10

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