AU2001218027A1 - Directed evolution of oxidase enzymes - Google Patents

Directed evolution of oxidase enzymes

Info

Publication number
AU2001218027A1
AU2001218027A1 AU2001218027A AU1802701A AU2001218027A1 AU 2001218027 A1 AU2001218027 A1 AU 2001218027A1 AU 2001218027 A AU2001218027 A AU 2001218027A AU 1802701 A AU1802701 A AU 1802701A AU 2001218027 A1 AU2001218027 A1 AU 2001218027A1
Authority
AU
Australia
Prior art keywords
mutation
galactose oxidase
polynucleotide
amino acid
nucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001218027A
Other languages
English (en)
Inventor
Frances H Arnold
Ioanna P. Petrounia
Lianhong Sun
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
California Institute of Technology CalTech
Original Assignee
IOANNA P PETROUNIA
California Institute of Technology CalTech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by IOANNA P PETROUNIA, California Institute of Technology CalTech filed Critical IOANNA P PETROUNIA
Publication of AU2001218027A1 publication Critical patent/AU2001218027A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AU2001218027A 2000-05-16 2000-11-27 Directed evolution of oxidase enzymes Abandoned AU2001218027A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/571,553 US7115403B1 (en) 2000-05-16 2000-05-16 Directed evolution of galactose oxidase enzymes
US09571553 2000-05-16
PCT/US2000/032345 WO2001088110A1 (en) 2000-05-16 2000-11-27 Directed evolution of oxidase enzymes

Publications (1)

Publication Number Publication Date
AU2001218027A1 true AU2001218027A1 (en) 2001-11-26

Family

ID=24284158

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001218027A Abandoned AU2001218027A1 (en) 2000-05-16 2000-11-27 Directed evolution of oxidase enzymes

Country Status (7)

Country Link
US (2) US7115403B1 (ja)
EP (1) EP1290147A4 (ja)
JP (1) JP2003533228A (ja)
AU (1) AU2001218027A1 (ja)
CA (1) CA2408060A1 (ja)
MX (1) MXPA02011232A (ja)
WO (1) WO2001088110A1 (ja)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1470219A4 (en) * 2001-04-16 2005-10-05 California Inst Of Techn PEROXIDE MOLDED CYTOCHROME OXYGENASE P450 OXYGENASE VARIANTS
AU2003230568A1 (en) 2002-02-27 2003-09-09 California Institute Of Technology Novel glucose 6-oxidases
EP1639091B1 (en) 2003-06-17 2012-12-05 California University Of Technology Regio- and enantioselective alkane hydroxylation with modified cytochrome p450
US8715988B2 (en) * 2005-03-28 2014-05-06 California Institute Of Technology Alkane oxidation by modified hydroxylases
CN101389791A (zh) 2005-12-23 2009-03-18 维文蒂阿生物技术股份有限公司 融合蛋白文库的产生和筛选方法及其应用
US8252559B2 (en) * 2006-08-04 2012-08-28 The California Institute Of Technology Methods and systems for selective fluorination of organic molecules
WO2008016709A2 (en) * 2006-08-04 2008-02-07 California Institute Of Technology Methods and systems for selective fluorination of organic molecules
WO2008121435A2 (en) * 2007-02-02 2008-10-09 The California Institute Of Technology Methods for generating novel stabilized proteins
US20080268517A1 (en) * 2007-02-08 2008-10-30 The California Institute Of Technology Stable, functional chimeric cytochrome p450 holoenzymes
US8802401B2 (en) * 2007-06-18 2014-08-12 The California Institute Of Technology Methods and compositions for preparation of selectively protected carbohydrates
US8309333B1 (en) 2009-11-16 2012-11-13 The United States of America, as represented by Department of Energy Regioselective alkane hydroxylation with a mutant AlkB enzyme
US8361769B1 (en) 2009-11-16 2013-01-29 U.S. Department Of Energy Regioselective alkane hydroxylation with a mutant CYP153A6 enzyme
US9322007B2 (en) 2011-07-22 2016-04-26 The California Institute Of Technology Stable fungal Cel6 enzyme variants
WO2020014049A1 (en) * 2018-07-09 2020-01-16 Codexis, Inc. Engineered galactose oxidase variant enzymes
JP2023544408A (ja) * 2020-10-06 2023-10-23 コデクシス， インコーポレイテッド 操作されたガラクトースオキシダーゼ改変体酵素
CN116355872B (zh) * 2023-02-23 2023-09-01 中国水产科学研究院黄海水产研究所 半乳糖氧化酶突变体gao-5f/ar、质粒、重组菌及其应用

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US5741691A (en) 1996-01-23 1998-04-21 California Institute Of Technology Para-nitrobenzyl esterases with enhanced activity in aqueous and nonaqueous media
US6096548A (en) 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
DE69835360T2 (de) 1997-01-17 2007-08-16 Maxygen, Inc., Redwood City EVOLUTION Prokaryotischer GANZER ZELLEN DURCH REKURSIVE SEQUENZREKOMBINATION
IL131966A0 (en) 1997-03-18 2001-03-19 Novo Industri As An in vitro method for construction of a dna library
US6153410A (en) 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
CN1308683A (zh) 1998-06-29 2001-08-15 菲洛斯公司 用于产生高多样性文库的方法
FR2782323B1 (fr) 1998-08-12 2002-01-11 Proteus Procede de production in vitro de sequences polynucleotidiques recombinees, banques de sequences et sequences ainsi obtenues
KR20010085850A (ko) 1998-09-29 2001-09-07 추후제출 코돈 변형 유전자의 재편성
US6090604A (en) * 1999-02-24 2000-07-18 Novo Nordisk Biotech, Inc. Polypeptides having galactose oxidase activity and nucleic acids encoding same
US6498026B2 (en) 2000-02-25 2002-12-24 Hercules Incorporated Variant galactose oxidase, nucleic acid encoding same, and methods of using same

Also Published As

Publication number Publication date
JP2003533228A (ja) 2003-11-11
US7115403B1 (en) 2006-10-03
EP1290147A1 (en) 2003-03-12
EP1290147A4 (en) 2004-12-08
MXPA02011232A (es) 2004-08-19
WO2001088110A1 (en) 2001-11-22
CA2408060A1 (en) 2001-11-22
US7098010B1 (en) 2006-08-29

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