AU1363088A - Method for the generation of antigen-specific t cell lines and therapeutic use thereof - Google Patents
Method for the generation of antigen-specific t cell lines and therapeutic use thereofInfo
- Publication number
- AU1363088A AU1363088A AU13630/88A AU1363088A AU1363088A AU 1363088 A AU1363088 A AU 1363088A AU 13630/88 A AU13630/88 A AU 13630/88A AU 1363088 A AU1363088 A AU 1363088A AU 1363088 A AU1363088 A AU 1363088A
- Authority
- AU
- Australia
- Prior art keywords
- antigen
- cells
- hcmv
- specific
- population
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 239000000427 antigen Substances 0.000 title claims description 264
- 108091007433 antigens Proteins 0.000 title claims description 264
- 102000036639 antigens Human genes 0.000 title claims description 264
- 238000000034 method Methods 0.000 title claims description 81
- 230000001225 therapeutic effect Effects 0.000 title description 17
- 210000004027 cell Anatomy 0.000 claims description 235
- 241000701024 Human betaherpesvirus 5 Species 0.000 claims description 191
- 210000005087 mononuclear cell Anatomy 0.000 claims description 124
- 210000001744 T-lymphocyte Anatomy 0.000 claims description 104
- 230000003612 virological effect Effects 0.000 claims description 68
- 210000002443 helper t lymphocyte Anatomy 0.000 claims description 56
- 230000035755 proliferation Effects 0.000 claims description 51
- 102000000588 Interleukin-2 Human genes 0.000 claims description 49
- 108010002350 Interleukin-2 Proteins 0.000 claims description 49
- 210000000612 antigen-presenting cell Anatomy 0.000 claims description 49
- 230000002062 proliferating effect Effects 0.000 claims description 49
- 230000001472 cytotoxic effect Effects 0.000 claims description 44
- 230000000735 allogeneic effect Effects 0.000 claims description 36
- 239000000203 mixture Substances 0.000 claims description 33
- 231100000433 cytotoxic Toxicity 0.000 claims description 27
- 210000002950 fibroblast Anatomy 0.000 claims description 22
- 230000001575 pathological effect Effects 0.000 claims description 20
- 241000701044 Human gammaherpesvirus 4 Species 0.000 claims description 16
- 241000124008 Mammalia Species 0.000 claims description 15
- DQJCDTNMLBYVAY-ZXXIYAEKSA-N (2S,5R,10R,13R)-16-{[(2R,3S,4R,5R)-3-{[(2S,3R,4R,5S,6R)-3-acetamido-4,5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy}-5-(ethylamino)-6-hydroxy-2-(hydroxymethyl)oxan-4-yl]oxy}-5-(4-aminobutyl)-10-carbamoyl-2,13-dimethyl-4,7,12,15-tetraoxo-3,6,11,14-tetraazaheptadecan-1-oic acid Chemical compound NCCCC[C@H](C(=O)N[C@@H](C)C(O)=O)NC(=O)CC[C@H](C(N)=O)NC(=O)[C@@H](C)NC(=O)C(C)O[C@@H]1[C@@H](NCC)C(O)O[C@H](CO)[C@H]1O[C@H]1[C@H](NC(C)=O)[C@@H](O)[C@H](O)[C@@H](CO)O1 DQJCDTNMLBYVAY-ZXXIYAEKSA-N 0.000 claims description 14
- 108010015899 Glycopeptides Proteins 0.000 claims description 14
- 102000002068 Glycopeptides Human genes 0.000 claims description 14
- 238000004519 manufacturing process Methods 0.000 claims description 14
- 239000002552 dosage form Substances 0.000 claims description 13
- 230000028993 immune response Effects 0.000 claims description 12
- 101710172711 Structural protein Proteins 0.000 claims description 9
- 108060003393 Granulin Proteins 0.000 claims description 8
- 210000004369 blood Anatomy 0.000 claims description 8
- 239000008280 blood Substances 0.000 claims description 8
- 230000002163 immunogen Effects 0.000 claims description 7
- 230000035899 viability Effects 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 5
- 238000007911 parenteral administration Methods 0.000 claims description 5
- 230000001717 pathogenic effect Effects 0.000 claims description 5
- 244000052769 pathogen Species 0.000 claims description 4
- 108010003533 Viral Envelope Proteins Proteins 0.000 claims description 3
- 210000004408 hybridoma Anatomy 0.000 claims description 3
- 210000004779 membrane envelope Anatomy 0.000 claims description 3
- 108010087302 Viral Structural Proteins Proteins 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 description 35
- 102000003886 Glycoproteins Human genes 0.000 description 28
- 102000004169 proteins and genes Human genes 0.000 description 28
- 210000001626 skin fibroblast Anatomy 0.000 description 27
- 108090000288 Glycoproteins Proteins 0.000 description 24
- 241000700605 Viruses Species 0.000 description 24
- 230000009696 proliferative response Effects 0.000 description 23
- 108700018351 Major Histocompatibility Complex Proteins 0.000 description 19
- 230000020382 suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I Effects 0.000 description 19
- 208000015181 infectious disease Diseases 0.000 description 17
- 210000000265 leukocyte Anatomy 0.000 description 14
- 238000011282 treatment Methods 0.000 description 13
- RJURFGZVJUQBHK-UHFFFAOYSA-N actinomycin D Natural products CC1OC(=O)C(C(C)C)N(C)C(=O)CN(C)C(=O)C2CCCN2C(=O)C(C(C)C)NC(=O)C1NC(=O)C1=C(N)C(=O)C(C)=C2OC(C(C)=CC=C3C(=O)NC4C(=O)NC(C(N5CCCC5C(=O)N(C)CC(=O)N(C)C(C(C)C)C(=O)OC4C)=O)C(C)C)=C3N=C21 RJURFGZVJUQBHK-UHFFFAOYSA-N 0.000 description 12
- 239000003599 detergent Substances 0.000 description 11
- 239000000284 extract Substances 0.000 description 10
- 238000002360 preparation method Methods 0.000 description 9
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 8
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 8
- YPHMISFOHDHNIV-FSZOTQKASA-N cycloheximide Chemical compound C1[C@@H](C)C[C@H](C)C(=O)[C@@H]1[C@H](O)CC1CC(=O)NC(=O)C1 YPHMISFOHDHNIV-FSZOTQKASA-N 0.000 description 8
- 238000010790 dilution Methods 0.000 description 8
- 239000012895 dilution Substances 0.000 description 8
- 239000012636 effector Substances 0.000 description 8
- 230000004044 response Effects 0.000 description 8
- 210000001519 tissue Anatomy 0.000 description 8
- 229930006000 Sucrose Natural products 0.000 description 7
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 7
- 210000003719 b-lymphocyte Anatomy 0.000 description 7
- 239000003795 chemical substances by application Substances 0.000 description 7
- 239000012894 fetal calf serum Substances 0.000 description 7
- 239000000047 product Substances 0.000 description 7
- 230000000638 stimulation Effects 0.000 description 7
- 239000005720 sucrose Substances 0.000 description 7
- 108010092160 Dactinomycin Proteins 0.000 description 6
- RJURFGZVJUQBHK-IIXSONLDSA-N actinomycin D Chemical compound C[C@H]1OC(=O)[C@H](C(C)C)N(C)C(=O)CN(C)C(=O)[C@@H]2CCCN2C(=O)[C@@H](C(C)C)NC(=O)[C@H]1NC(=O)C1=C(N)C(=O)C(C)=C2OC(C(C)=CC=C3C(=O)N[C@@H]4C(=O)N[C@@H](C(N5CCC[C@H]5C(=O)N(C)CC(=O)N(C)[C@@H](C(C)C)C(=O)O[C@@H]4C)=O)C(C)C)=C3N=C21 RJURFGZVJUQBHK-IIXSONLDSA-N 0.000 description 6
- 230000003190 augmentative effect Effects 0.000 description 6
- 239000000872 buffer Substances 0.000 description 6
- 229960000640 dactinomycin Drugs 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 206010022000 influenza Diseases 0.000 description 6
- 230000000670 limiting effect Effects 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- 230000007420 reactivation Effects 0.000 description 6
- 230000009257 reactivity Effects 0.000 description 6
- 208000036142 Viral infection Diseases 0.000 description 5
- 230000000890 antigenic effect Effects 0.000 description 5
- 230000008901 benefit Effects 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 210000001151 cytotoxic T lymphocyte Anatomy 0.000 description 5
- 238000004128 high performance liquid chromatography Methods 0.000 description 5
- 238000009169 immunotherapy Methods 0.000 description 5
- 238000000338 in vitro Methods 0.000 description 5
- 239000002245 particle Substances 0.000 description 5
- 210000002966 serum Anatomy 0.000 description 5
- 239000006228 supernatant Substances 0.000 description 5
- 230000009385 viral infection Effects 0.000 description 5
- 108700016947 Bos taurus structural-GP Proteins 0.000 description 4
- 206010011831 Cytomegalovirus infection Diseases 0.000 description 4
- 102100037840 Dehydrogenase/reductase SDR family member 2, mitochondrial Human genes 0.000 description 4
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 4
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 4
- 102100034349 Integrase Human genes 0.000 description 4
- 101710188053 Protein D Proteins 0.000 description 4
- 101710132893 Resolvase Proteins 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 210000005105 peripheral blood lymphocyte Anatomy 0.000 description 4
- 239000002244 precipitate Substances 0.000 description 4
- 238000002560 therapeutic procedure Methods 0.000 description 4
- 238000013518 transcription Methods 0.000 description 4
- 230000035897 transcription Effects 0.000 description 4
- 241000712461 unidentified influenza virus Species 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- 208000003322 Coinfection Diseases 0.000 description 3
- 101710121417 Envelope glycoprotein Proteins 0.000 description 3
- 108700002232 Immediate-Early Genes Proteins 0.000 description 3
- 102000008072 Lymphokines Human genes 0.000 description 3
- 108010074338 Lymphokines Proteins 0.000 description 3
- 102000043129 MHC class I family Human genes 0.000 description 3
- 108091054437 MHC class I family Proteins 0.000 description 3
- 206010028980 Neoplasm Diseases 0.000 description 3
- 108090001074 Nucleocapsid Proteins Proteins 0.000 description 3
- 239000012980 RPMI-1640 medium Substances 0.000 description 3
- 239000006146 Roswell Park Memorial Institute medium Substances 0.000 description 3
- 101000999689 Saimiriine herpesvirus 2 (strain 11) Transcriptional regulator ICP22 homolog Proteins 0.000 description 3
- 230000001154 acute effect Effects 0.000 description 3
- 230000003416 augmentation Effects 0.000 description 3
- 201000011510 cancer Diseases 0.000 description 3
- 230000004663 cell proliferation Effects 0.000 description 3
- 230000009089 cytolysis Effects 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 238000000684 flow cytometry Methods 0.000 description 3
- 230000005714 functional activity Effects 0.000 description 3
- 210000000987 immune system Anatomy 0.000 description 3
- 230000004073 interleukin-2 production Effects 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 210000004698 lymphocyte Anatomy 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 238000013207 serial dilution Methods 0.000 description 3
- ILXAOQAXSHVHTM-UHFFFAOYSA-M sodium;2-amino-2-(hydroxymethyl)propane-1,3-diol;chloride Chemical compound [Na+].[Cl-].OCC(N)(CO)CO ILXAOQAXSHVHTM-UHFFFAOYSA-M 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 238000010600 3H thymidine incorporation assay Methods 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000724252 Cucumber mosaic virus Species 0.000 description 2
- 101710088235 Envelope glycoprotein C homolog Proteins 0.000 description 2
- 108010007403 Immediate-Early Proteins Proteins 0.000 description 2
- 241001529936 Murinae Species 0.000 description 2
- NWIBSHFKIJFRCO-WUDYKRTCSA-N Mytomycin Chemical compound C1N2C(C(C(C)=C(N)C3=O)=O)=C3[C@@H](COC(N)=O)[C@@]2(OC)[C@@H]2[C@H]1N2 NWIBSHFKIJFRCO-WUDYKRTCSA-N 0.000 description 2
- 230000005867 T cell response Effects 0.000 description 2
- 229920004890 Triton X-100 Polymers 0.000 description 2
- 239000013504 Triton X-100 Substances 0.000 description 2
- 108010067390 Viral Proteins Proteins 0.000 description 2
- 238000005571 anion exchange chromatography Methods 0.000 description 2
- 230000007503 antigenic stimulation Effects 0.000 description 2
- 239000002246 antineoplastic agent Substances 0.000 description 2
- 229940041181 antineoplastic drug Drugs 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000012620 biological material Substances 0.000 description 2
- 210000000234 capsid Anatomy 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 238000001516 cell proliferation assay Methods 0.000 description 2
- 230000005859 cell recognition Effects 0.000 description 2
- 238000002659 cell therapy Methods 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 230000001461 cytolytic effect Effects 0.000 description 2
- 230000003013 cytotoxicity Effects 0.000 description 2
- 231100000135 cytotoxicity Toxicity 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000002523 gelfiltration Methods 0.000 description 2
- 102000034356 gene-regulatory proteins Human genes 0.000 description 2
- 108091006104 gene-regulatory proteins Proteins 0.000 description 2
- 239000003365 glass fiber Substances 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 210000002540 macrophage Anatomy 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 210000001978 pro-t lymphocyte Anatomy 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 238000011321 prophylaxis Methods 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000004007 reversed phase HPLC Methods 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 239000002356 single layer Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 2
- 230000002269 spontaneous effect Effects 0.000 description 2
- 210000000130 stem cell Anatomy 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 229960000814 tetanus toxoid Drugs 0.000 description 2
- 239000003104 tissue culture media Substances 0.000 description 2
- 230000036642 wellbeing Effects 0.000 description 2
- IQFYYKKMVGJFEH-OFKYTIFKSA-N 1-[(2r,4s,5r)-4-hydroxy-5-(tritiooxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione Chemical compound C1[C@H](O)[C@@H](CO[3H])O[C@H]1N1C(=O)NC(=O)C(C)=C1 IQFYYKKMVGJFEH-OFKYTIFKSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- MSWZFWKMSRAUBD-IVMDWMLBSA-N 2-amino-2-deoxy-D-glucopyranose Chemical compound N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O MSWZFWKMSRAUBD-IVMDWMLBSA-N 0.000 description 1
- 206010067484 Adverse reaction Diseases 0.000 description 1
- 241000712891 Arenavirus Species 0.000 description 1
- 208000023275 Autoimmune disease Diseases 0.000 description 1
- 102000019260 B-Cell Antigen Receptors Human genes 0.000 description 1
- 108010012919 B-Cell Antigen Receptors Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000222122 Candida albicans Species 0.000 description 1
- 230000004543 DNA replication Effects 0.000 description 1
- 230000006820 DNA synthesis Effects 0.000 description 1
- 101710091045 Envelope protein Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 208000031637 Erythroblastic Acute Leukemia Diseases 0.000 description 1
- 208000036566 Erythroleukaemia Diseases 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 102100036242 HLA class II histocompatibility antigen, DQ alpha 2 chain Human genes 0.000 description 1
- 101710154606 Hemagglutinin Proteins 0.000 description 1
- 108010088652 Histocompatibility Antigens Class I Proteins 0.000 description 1
- 102000008949 Histocompatibility Antigens Class I Human genes 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 101000930801 Homo sapiens HLA class II histocompatibility antigen, DQ alpha 2 chain Proteins 0.000 description 1
- 101000972485 Homo sapiens Lupus La protein Proteins 0.000 description 1
- 206010062016 Immunosuppression Diseases 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- 241000712899 Lymphocytic choriomeningitis mammarenavirus Species 0.000 description 1
- 102000043131 MHC class II family Human genes 0.000 description 1
- 108091054438 MHC class II family Proteins 0.000 description 1
- 101710141347 Major envelope glycoprotein Proteins 0.000 description 1
- 108010090054 Membrane Glycoproteins Proteins 0.000 description 1
- 102000012750 Membrane Glycoproteins Human genes 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 241000186362 Mycobacterium leprae Species 0.000 description 1
- 208000001388 Opportunistic Infections Diseases 0.000 description 1
- 101710093908 Outer capsid protein VP4 Proteins 0.000 description 1
- 101710135467 Outer capsid protein sigma-1 Proteins 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 206010035664 Pneumonia Diseases 0.000 description 1
- 101710186352 Probable membrane antigen 3 Proteins 0.000 description 1
- 101710181078 Probable membrane antigen 75 Proteins 0.000 description 1
- 101710176177 Protein A56 Proteins 0.000 description 1
- 101710188315 Protein X Proteins 0.000 description 1
- 108010004487 SS-B antigen Proteins 0.000 description 1
- 241000700584 Simplexvirus Species 0.000 description 1
- 230000024932 T cell mediated immunity Effects 0.000 description 1
- 101710178472 Tegument protein Proteins 0.000 description 1
- 206010043376 Tetanus Diseases 0.000 description 1
- 206010046865 Vaccinia virus infection Diseases 0.000 description 1
- 206010058874 Viraemia Diseases 0.000 description 1
- 108700005077 Viral Genes Proteins 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 208000021841 acute erythroid leukemia Diseases 0.000 description 1
- 230000006838 adverse reaction Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000030741 antigen processing and presentation Effects 0.000 description 1
- MSWZFWKMSRAUBD-UHFFFAOYSA-N beta-D-galactosamine Natural products NC1C(O)OC(CO)C(O)C1O MSWZFWKMSRAUBD-UHFFFAOYSA-N 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 229940095731 candida albicans Drugs 0.000 description 1
- 230000020411 cell activation Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000009260 cross reactivity Effects 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 230000000120 cytopathologic effect Effects 0.000 description 1
- 230000007402 cytotoxic response Effects 0.000 description 1
- 238000002784 cytotoxicity assay Methods 0.000 description 1
- 231100000263 cytotoxicity test Toxicity 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 230000008260 defense mechanism Effects 0.000 description 1
- 210000004443 dendritic cell Anatomy 0.000 description 1
- 230000002500 effect on skin Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 210000003953 foreskin Anatomy 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 229960002442 glucosamine Drugs 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 239000000185 hemagglutinin Substances 0.000 description 1
- 102000052972 human La Human genes 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 230000004727 humoral immunity Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 230000008073 immune recognition Effects 0.000 description 1
- 230000037451 immune surveillance Effects 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 230000016784 immunoglobulin production Effects 0.000 description 1
- 238000001114 immunoprecipitation Methods 0.000 description 1
- 230000001506 immunosuppresive effect Effects 0.000 description 1
- 230000001024 immunotherapeutic effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000015788 innate immune response Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000000464 low-speed centrifugation Methods 0.000 description 1
- 210000003738 lymphoid progenitor cell Anatomy 0.000 description 1
- 210000003810 lymphokine-activated killer cell Anatomy 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- FBBDOOHMGLLEGJ-UHFFFAOYSA-N methane;hydrochloride Chemical compound C.Cl FBBDOOHMGLLEGJ-UHFFFAOYSA-N 0.000 description 1
- 229960004857 mitomycin Drugs 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000002625 monoclonal antibody therapy Methods 0.000 description 1
- 210000000822 natural killer cell Anatomy 0.000 description 1
- 210000005170 neoplastic cell Anatomy 0.000 description 1
- 230000001613 neoplastic effect Effects 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 229920002113 octoxynol Polymers 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000001959 radiotherapy Methods 0.000 description 1
- 238000000472 rate-zonal centrifugation Methods 0.000 description 1
- 238000006722 reduction reaction Methods 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000000284 resting effect Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 238000001542 size-exclusion chromatography Methods 0.000 description 1
- 238000007390 skin biopsy Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000007910 systemic administration Methods 0.000 description 1
- 238000009121 systemic therapy Methods 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000014723 transformation of host cell by virus Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 238000011277 treatment modality Methods 0.000 description 1
- 230000002476 tumorcidal effect Effects 0.000 description 1
- 241001529453 unidentified herpesvirus Species 0.000 description 1
- 208000007089 vaccinia Diseases 0.000 description 1
- 210000002845 virion Anatomy 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0636—T lymphocytes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/46—Cellular immunotherapy
- A61K39/461—Cellular immunotherapy characterised by the cell type used
- A61K39/4611—T-cells, e.g. tumor infiltrating lymphocytes [TIL], lymphokine-activated killer cells [LAK] or regulatory T cells [Treg]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/46—Cellular immunotherapy
- A61K39/464—Cellular immunotherapy characterised by the antigen targeted or presented
- A61K39/464838—Viral antigens
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/16011—Herpesviridae
- C12N2710/16111—Cytomegalovirus, e.g. human herpesvirus 5
- C12N2710/16134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Veterinary Medicine (AREA)
- Biotechnology (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Mycology (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Zoology (AREA)
- Epidemiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Hematology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US2457187A | 1987-03-11 | 1987-03-11 | |
| US024571 | 1987-03-11 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU1363088A true AU1363088A (en) | 1988-10-10 |
Family
ID=21821277
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU13630/88A Abandoned AU1363088A (en) | 1987-03-11 | 1988-02-09 | Method for the generation of antigen-specific t cell lines and therapeutic use thereof |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0348413A1 (ko) |
| JP (1) | JPH02502424A (ko) |
| KR (1) | KR890700659A (ko) |
| AU (1) | AU1363088A (ko) |
| WO (1) | WO1988007077A1 (ko) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6207161B1 (en) | 1986-07-16 | 2001-03-27 | City Of Hope | Induction of cytolytic T-lymphocytes with cytomegalovirus polypeptides |
| AU2925789A (en) * | 1987-12-17 | 1989-07-19 | Browning's Clinical Pathology Services Limited | Lymphokine activation of cells for adoptive immunotherapy, e.g. of hiv infection |
| US6905680B2 (en) | 1988-11-23 | 2005-06-14 | Genetics Institute, Inc. | Methods of treating HIV infected subjects |
| US6534055B1 (en) | 1988-11-23 | 2003-03-18 | Genetics Institute, Inc. | Methods for selectively stimulating proliferation of T cells |
| US7479269B2 (en) | 1988-11-23 | 2009-01-20 | Genetics Institute, Llc | Methods for selectively enriching TH1 and TH2 cells |
| JP2639834B2 (ja) * | 1988-11-30 | 1997-08-13 | 有限会社ジオリサーチ | 免疫記憶細胞懸濁液およびその調製方法 |
| DE3921211C1 (ko) * | 1989-06-28 | 1990-11-29 | Idt Ag Fuer In Vivo Diagnostik Und Therapie, Zuerich, Ch | |
| WO1992020783A1 (de) * | 1991-05-16 | 1992-11-26 | Idt Ag Für In Vivo Diagnostik Und Therapie | Verfahren zur bereitstellung von antigen-spezifischen b- und t-lymphozyten sowie davon erhaltene monoklonale antikörper |
| JPH06510799A (ja) * | 1992-06-25 | 1994-12-01 | シティ・オブ・ホープ | サイトメガロウイルスのポリペプチドによる細胞溶解性ｔ−リンパ球の誘導 |
| US7175843B2 (en) | 1994-06-03 | 2007-02-13 | Genetics Institute, Llc | Methods for selectively stimulating proliferation of T cells |
| US5827642A (en) * | 1994-08-31 | 1998-10-27 | Fred Hutchinson Cancer Research Center | Rapid expansion method ("REM") for in vitro propagation of T lymphocytes |
| IL125884A0 (en) * | 1996-03-04 | 1999-04-11 | Targeted Genetics Corp | Modified rapid expansion methods ("modified-rem") for in vitro propagation of t lymphocytes |
| EP0904350B1 (en) | 1996-03-04 | 2010-08-04 | Calyx Bio-Ventures Inc. | Modified rapid expansion methods ("modified-rem") for in vitro propagation of t lymphocytes |
| CA2335516A1 (en) * | 1998-06-26 | 2000-01-06 | Keld Kaltoft | Methods of expanding and selecting disease associated t-cells |
-
1988
- 1988-02-09 EP EP88902046A patent/EP0348413A1/en not_active Withdrawn
- 1988-02-09 AU AU13630/88A patent/AU1363088A/en not_active Abandoned
- 1988-02-09 JP JP63502143A patent/JPH02502424A/ja active Pending
- 1988-02-09 WO PCT/US1988/000383 patent/WO1988007077A1/en not_active Application Discontinuation
- 1988-11-11 KR KR1019880701443A patent/KR890700659A/ko not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| EP0348413A1 (en) | 1990-01-03 |
| WO1988007077A1 (en) | 1988-09-22 |
| KR890700659A (ko) | 1989-04-26 |
| JPH02502424A (ja) | 1990-08-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Nikiforow et al. | Cytolytic CD4+-T-cell clones reactive to EBNA1 inhibit Epstein-Barr virus-induced B-cell proliferation | |
| Szmania et al. | Isolation and expansion of cytomegalovirus-specific cytotoxic T lymphocytes to clinical scale from a single blood draw using dendritic cells and HLA-tetramers | |
| Engers et al. | Inhibition of T cell-mediated functions by MVM (i), a parvovirus closely related to minute virus of mice. | |
| KR102190890B1 (ko) | 암 치료를 위한 t 세포의 활성화 방법 | |
| Burns et al. | Epstein–Barr virus-specific cytotoxic T-lymphocytes for adoptive immunotherapy of post-transplant lymphoproliferative disease | |
| Nandakumar et al. | Natural killer cells as novel helpers in anti-herpes simplex virus immune response | |
| AU1363088A (en) | Method for the generation of antigen-specific t cell lines and therapeutic use thereof | |
| Yasukawa et al. | Functional heterogeneity among herpes simplex virus-specific human CD4+ T cells. | |
| Lee et al. | Cell-mediated immunity (CMI) to human wart virus and wart-associated tissue antigens. | |
| Moody et al. | Lymphocyte transformation induced by autologous cells. VIII. Impaired autologous mixed lymphocyte reactivity in patients with acute infectious mononucleosis. | |
| NZ282348A (en) | Cytotoxic epstein-barr virus t-cell epitopes and medicaments | |
| EP0332659A1 (en) | Immunogenic glycopeptides of human cytomegalovirus | |
| JPH08510118A (ja) | 新規なマクロファージ、その製造方法、及び医薬組成物の活性物質としてのその用途 | |
| Alfieri et al. | Lytic, nontransforming Epstein-Barr virus (EBV) from a patient with chronic active EBV infection. | |
| Takamatsu et al. | Cultivation of bluetongue virus-specific ovine T cells and their cross-reactivity with different serotype viruses. | |
| Avila‐Carino et al. | Paired Epstein‐Barr virus (EBV)‐negative and EBV‐converted Burkitt lymphoma lines: Stimulatory capacity in allogeneic mixed lymphocyte cultures | |
| Kurane et al. | Characterization with monoclonal antibodies of human lymphocytes active in natural killing and antibody-dependent cell-mediated cytotoxicity of dengue virus-infected cells. | |
| Hughes et al. | Immunopotentiation of bovine herpes virus subunit vaccination by interleukin-2. | |
| Moretta et al. | High frequency of Epstein–Barr virus (EBV) lymphoblastoid cell line‐reactive lymphocytes in cord blood: evaluation of cytolytic activity and IL‐2 production | |
| KR20230104844A (ko) | 암 치료를 위한 t 세포의 활성화 방법 | |
| Wiryana et al. | Augmentation of cell-mediated immunotherapy against herpes simplex virus by interleukins: comparison of in vivo effects of IL-2 and IL-7 on adoptively transferred T cells | |
| SULLIVAN | Epstein-Barr virus and the X-linked lymphoproliferative syndrome | |
| Moss et al. | EBV-specific immune response: early research and personal reminiscences | |
| US5248768A (en) | Immunogenic glycoproteins of human cytomegalovirus | |
| Kishimoto et al. | Protection against murine coxsackievirus B3 myocarditis by T cell vaccination |