ATE96230T1 - Verfaerbungsverfahren fuer proteine und nukleinsaeuren. - Google Patents

Verfaerbungsverfahren fuer proteine und nukleinsaeuren.

Info

Publication number
ATE96230T1
ATE96230T1 AT85200854T AT85200854T ATE96230T1 AT E96230 T1 ATE96230 T1 AT E96230T1 AT 85200854 T AT85200854 T AT 85200854T AT 85200854 T AT85200854 T AT 85200854T AT E96230 T1 ATE96230 T1 AT E96230T1
Authority
AT
Austria
Prior art keywords
proteins
nucleic acids
staining procedures
metal particles
colloidal metal
Prior art date
Application number
AT85200854T
Other languages
English (en)
Inventor
Marc Karel Julia Joz Moeremans
Guido Franciscus Theop Daneels
Raeymaeker Marc Carl De
Mey Jan Raoul De
Original Assignee
Janssen Pharmaceutica Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Janssen Pharmaceutica Nv filed Critical Janssen Pharmaceutica Nv
Application granted granted Critical
Publication of ATE96230T1 publication Critical patent/ATE96230T1/de

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6827Total protein determination, e.g. albumin in urine
    • G01N33/683Total protein determination, e.g. albumin in urine involving metal ions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44704Details; Accessories
    • G01N27/44717Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6827Total protein determination, e.g. albumin in urine
    • G01N33/683Total protein determination, e.g. albumin in urine involving metal ions
    • G01N33/6836Silver staining
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00Chemistry: analytical and immunological testing
    • Y10T436/14Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333Saccharide [e.g., DNA, etc.]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Organic Chemistry (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT85200854T 1984-06-22 1985-05-29 Verfaerbungsverfahren fuer proteine und nukleinsaeuren. ATE96230T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB848415998A GB8415998D0 (en) 1984-06-22 1984-06-22 Staining method
EP85200854A EP0165633B1 (de) 1984-06-22 1985-05-29 Verfärbungsverfahren für Proteine und Nukleinsäuren

Publications (1)

Publication Number Publication Date
ATE96230T1 true ATE96230T1 (de) 1993-11-15

Family

ID=10562845

Family Applications (1)

Application Number Title Priority Date Filing Date
AT85200854T ATE96230T1 (de) 1984-06-22 1985-05-29 Verfaerbungsverfahren fuer proteine und nukleinsaeuren.

Country Status (8)

Country Link
US (1) US4920059A (de)
EP (1) EP0165633B1 (de)
JP (1) JPH076991B2 (de)
AT (1) ATE96230T1 (de)
AU (1) AU599304B2 (de)
CA (1) CA1265730A (de)
DE (1) DE3587633T2 (de)
GB (1) GB8415998D0 (de)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1240736A (en) * 1985-12-19 1988-08-16 Robert E. Emmons Use of dry analytical elements to determine electrically separated analytes
FR2598435A1 (fr) * 1986-05-06 1987-11-13 Clonatec Sa Procede et dispositif pour la detection de genomes viraux a adn et arn dans les milieux biologiques, notamment dans le serum sanguin
AU602694B2 (en) * 1986-06-09 1990-10-25 Ortho Diagnostic Systems Inc. Improved colloidal gold membrane assay
US5491098A (en) * 1987-03-09 1996-02-13 Janssen Pharmaceutica N.V. Method for depositing metal particles on a marker
CA1340803C (en) * 1987-03-09 1999-10-26 Janssen Pharmaceutica N.V. Method for depositing metal particles on a marker
JPS63274865A (ja) * 1987-05-06 1988-11-11 Sachimaru Senoo 酸性ないし中性で安定な正及び負荷電の鉄コロイド溶液
JP2581566B2 (ja) * 1987-09-30 1997-02-12 和光純薬工業株式会社 鉄コロイド標識抗体及びその製法並びにこれを用いる組織細胞化学的検出方法
DE3878167T2 (de) * 1987-11-16 1993-05-27 Janssen Pharmaceutica Nv Auf ultrakleine kolloidale metallteilchen gegruendetes, allgemein anwendbares nachweissystem.
JPH01132870A (ja) * 1987-11-18 1989-05-25 Toyo Cloth Co Ltd 鮮明な縦皺模様を有する布地の製造方法
AU7688191A (en) * 1990-03-28 1991-10-21 Board Of Regents, The University Of Texas System Production of electrophoretically-uniform protein-colloidal gold complexes
US5298135A (en) * 1992-07-07 1994-03-29 Board Of Regents, The University Of Texas System Production of electrophoretically-homogenous protein-colloidal gold complexes
US5472881A (en) * 1992-11-12 1995-12-05 University Of Utah Research Foundation Thiol labeling of DNA for attachment to gold surfaces
WO1996005326A1 (en) * 1994-08-17 1996-02-22 Fox John S Method and apparatus for magnetically detecting proteins and nucleic acids
US5851777A (en) * 1996-02-05 1998-12-22 Dade Behring Inc. Homogeneous sol-sol assay
ES2308800T3 (es) * 1997-01-08 2008-12-01 Invitrogen Corporation Metodos para la produccion de proteinas.
US6699507B1 (en) * 1999-08-05 2004-03-02 Wisconsin Alulmni Research Foundation Colloidal particles of different element composition for specific labeling purposes
CA2381732A1 (en) * 1999-08-21 2001-03-01 John S. Fox High sensitivity biomolecule detection with magnetic particles
US7192778B2 (en) * 1999-10-06 2007-03-20 Natan Michael J Surface enhanced spectroscopy-active composite nanoparticles
US8497131B2 (en) * 1999-10-06 2013-07-30 Becton, Dickinson And Company Surface enhanced spectroscopy-active composite nanoparticles comprising Raman-active reporter molecules
US6743639B1 (en) 1999-10-13 2004-06-01 Nve Corporation Magnetizable bead detector
US6875621B2 (en) 1999-10-13 2005-04-05 Nve Corporation Magnetizable bead detector
US6861263B2 (en) 2001-01-26 2005-03-01 Surromed, Inc. Surface-enhanced spectroscopy-active sandwich nanoparticles
US20060263897A1 (en) * 2003-09-09 2006-11-23 Koninklijke Philips Electronics N.V. Nanoparticles for detecting analytes
US7391091B2 (en) 2004-09-29 2008-06-24 Nve Corporation Magnetic particle flow detector
DE102005044286A1 (de) * 2005-09-16 2007-04-05 GM Global Technology Operations, Inc., Detroit Kraftfahrzeug mit einem Spritzwasserbehälter
US20090298197A1 (en) * 2005-11-15 2009-12-03 Oxonica Materials Inc. Sers-based methods for detection of bioagents
US8409863B2 (en) 2005-12-14 2013-04-02 Becton, Dickinson And Company Nanoparticulate chemical sensors using SERS
US7723100B2 (en) 2006-01-13 2010-05-25 Becton, Dickinson And Company Polymer coated SERS nanotag
ES2601391T3 (es) * 2006-01-27 2017-02-15 Becton Dickinson And Company Inmunoensayo de flujo lateral con modalidad de detección encapsulada
EP2069386A4 (de) 2006-07-21 2009-10-28 Life Technologies Corp Proteinmolekülgewichtsstandards mit hoher trennschärfe
ES2649986T3 (es) * 2006-07-24 2018-01-16 Becton Dickinson And Company Aparato y procedimiento para la concentración de partículas de ensayo
WO2009067603A1 (en) * 2007-11-21 2009-05-28 Bio-Rad Laboratories, Inc. Photoluminescent metal complexes for protein staining
WO2013063147A1 (en) 2011-10-24 2013-05-02 Atossa Genetics, Inc. Absorbent paper and use thereof for breast cancer detection

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE869520C (de) * 1944-08-27 1953-03-05 Aeg Verfahren zur uebermikroskopischen Abbildung von Mikroorganismen
NL7807532A (nl) * 1978-07-13 1980-01-15 Akzo Nv Metaal-immunotest.
NL8000173A (nl) * 1980-01-11 1981-08-03 Akzo Nv Toepassing van in water dispergeerbare, hydrofobe kleurstoffen als label in immunochemische testen.
US4416998A (en) * 1981-04-02 1983-11-22 The Upjohn Company Silver stain procedure and kit for same
FI821071A7 (fi) * 1981-04-02 1982-10-03 Health Products Inc Hopeaa sitovan aineen värjäysmenetelmä ja -välinesarja.
JPS58205855A (ja) * 1982-05-27 1983-11-30 Tokyo Seikagaku Kenkyukai 蛋白質の分析方法および分析用キツト
GB8331514D0 (en) * 1983-11-25 1984-01-04 Janssen Pharmaceutica Nv Visualization method
US4555490A (en) * 1984-06-08 1985-11-26 The United States Of America As Represented By The Department Of Health And Human Services Rapid visualization system for gel electrophoresis
US4752567A (en) * 1984-06-21 1988-06-21 Janssen Pharmaceutica N.V. Method of visualizing individual submicroscopic metal particles

Also Published As

Publication number Publication date
EP0165633B1 (de) 1993-10-20
JPH076991B2 (ja) 1995-01-30
DE3587633T2 (de) 1994-02-17
US4920059A (en) 1990-04-24
GB8415998D0 (en) 1984-07-25
JPS6117958A (ja) 1986-01-25
CA1265730A (en) 1990-02-13
DE3587633D1 (de) 1993-11-25
AU4339785A (en) 1986-01-02
AU599304B2 (en) 1990-07-19
EP0165633A3 (en) 1988-11-09
EP0165633A2 (de) 1985-12-27

Similar Documents

Publication Publication Date Title
ATE96230T1 (de) Verfaerbungsverfahren fuer proteine und nukleinsaeuren.
Pacaud et al. Isolation and some properties of a proteolytic enzyme from Escherichia coli (protease I)
FI863951L (fi) Menetelmä tärkkelyshydrolysaatin suodatettavuuden parantamiseksi ja siihen käytettävä entsyymikoostumus
JPS54143297A (en) Method of detecting and identifying nucleic acid or arrangement thereof* and enzyme reacting substance for said method
FI893702A7 (fi) Menetelmä ihmisen seerumialbumiinin ja muiden heterologisten proteiinien mikrobiologiseksi valmistamiseksi hiivasta
IL60888A (en) Substrates and process for the quantitative assay of enzymes
DE68912754D1 (de) Verfahren und Vorrichtung zum gerrichteten Heizen von Glas.
PT83451A (pt) Process for the preparation of derivatives of quinoline benzothiazole and analogous thereof
NO171460C (no) Blanding for inhibering av jern- og staalkorrosjon og anvendelse dera
IL71699A0 (en) Methods and kits for the analysis and detection of biological or genetic material
NO175299C (no) Fremgangsmåte for kontinuerlig raffinering av silisium
FR2609723B1 (fr) Procede de preparation d'une enzyme ou d'un micro-organisme immobilise
FR2509737B1 (fr) Procede de preparation d'une composition organo-silicique avec inhibiteur de durcissement produit in situ et composition ainsi produite
JPS5632999A (en) Experimental method of control zone test in aqueous culture medium
NO850074L (no) Fremgangsmaate for avvanning av mineralkonsentrater
White et al. Identification of aspirinase with one of the carboxylesterases requiring a thiol group
PT88436A (pt) Process for the preparation of modified plasminogen activators
NO803907L (no) Fremgangsmaate for pneumatisk overflateraffinering av staal uten overloep av slagg
FR2509139B1 (fr) Isolement de proteine microbienne d'une teneur reduite en acides nucleiques
AU605754B2 (en) Process for acylating amines with 2-benzothiazolyl thioesters of carboxylic acids
IT1111665B (it) Metodi e reattivi per l'analisi di ferro e di proteine leganti ferro
AT381789B (de) Nachwaermofen zum nachwaermen und zur temperaturvergleichmaessigung von heissem stahlgut
FI911557A7 (fi) Menetelmä nukleiinihappotemplaattien spesifiseen monistamiseen ja nukleiinihappojen määrittämiseen
FR2567640B1 (fr) Sonde pour le controle des defauts superficiels de corps metalliques a temperature elevee
Le Deaut et al. Purification of a soluble ATPase of rat liver

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
EELA Cancelled due to lapse of time