ATE85812T1 - Verkuerzter phosphoglycerat-kinase-promotor. - Google Patents
Verkuerzter phosphoglycerat-kinase-promotor.Info
- Publication number
- ATE85812T1 ATE85812T1 AT86907170T AT86907170T ATE85812T1 AT E85812 T1 ATE85812 T1 AT E85812T1 AT 86907170 T AT86907170 T AT 86907170T AT 86907170 T AT86907170 T AT 86907170T AT E85812 T1 ATE85812 T1 AT E85812T1
- Authority
- AT
- Austria
- Prior art keywords
- promoter
- structural gene
- expression
- gene
- pgk
- Prior art date
Links
- 102000011755 Phosphoglycerate Kinase Human genes 0.000 title 1
- 101001099217 Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) Triosephosphate isomerase Proteins 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 abstract 4
- 239000003550 marker Substances 0.000 abstract 3
- 239000013612 plasmid Substances 0.000 abstract 3
- 101150047627 pgk gene Proteins 0.000 abstract 2
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 abstract 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 abstract 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 239000002207 metabolite Substances 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 230000010076 replication Effects 0.000 abstract 1
- 230000028327 secretion Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
- C07K14/43—Sweetening agents, e.g. thaumatin, monellin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/69—Increasing the copy number of the vector
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Mycology (AREA)
- Gastroenterology & Hepatology (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Valve Device For Special Equipments (AREA)
- Valve-Gear Or Valve Arrangements (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Luminescent Compositions (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Ignition Installations For Internal Combustion Engines (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US79747785A | 1985-11-13 | 1985-11-13 | |
| PCT/US1986/002443 WO1987003008A1 (en) | 1985-11-13 | 1986-11-13 | Shortened phosphoglycerate kinase promoter |
| EP86907170A EP0245479B1 (de) | 1985-11-13 | 1986-11-13 | Verkürzter phosphoglycerat-kinase-promotor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE85812T1 true ATE85812T1 (de) | 1993-03-15 |
Family
ID=25170940
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT86907170T ATE85812T1 (de) | 1985-11-13 | 1986-11-13 | Verkuerzter phosphoglycerat-kinase-promotor. |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP0245479B1 (de) |
| JP (1) | JPS63501767A (de) |
| AT (1) | ATE85812T1 (de) |
| AU (1) | AU621277B2 (de) |
| CA (1) | CA1312838C (de) |
| DE (1) | DE3687796T2 (de) |
| WO (1) | WO1987003008A1 (de) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL83435A (en) * | 1986-09-23 | 1993-01-14 | Miles Lab | Dna construct for expression of genes in yeast, an expression vector containing it and yeast transformed with said expression vector |
| DE3888561T2 (de) * | 1987-03-23 | 1994-09-01 | Zymogenetics Inc | Hohe Proteinsyntheserate in Hefe. |
| US5221624A (en) * | 1988-11-08 | 1993-06-22 | International Genetic Engineering, Inc. | DNA encoding (Lys46, Asp97, Asp113) and (Lys46, Asp.sup.137) thaumatin I polypeptides |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK368882A (da) * | 1981-08-25 | 1983-02-26 | Alan John Kingsman | Expessions vektorer |
| AU561343B2 (en) * | 1981-10-19 | 1987-05-07 | Genentech Inc. | Human immune interferon by recombinant dna |
| WO1984004538A1 (fr) * | 1983-05-19 | 1984-11-22 | Unilever Nv | Ameliorations apportees a l'expression de genes fraichement introduits dans des cellules de levure |
| GB8314961D0 (en) * | 1983-05-31 | 1983-07-06 | Kingsman A J | Dna sequence |
| GB8521496D0 (en) * | 1985-08-29 | 1985-10-02 | Ciba Geigy Ag | Repressible yeast promoters |
| GB8620926D0 (en) * | 1986-08-29 | 1986-10-08 | Delta Biotechnology Ltd | Yeast promoter |
-
1986
- 1986-11-13 AT AT86907170T patent/ATE85812T1/de not_active IP Right Cessation
- 1986-11-13 JP JP61506097A patent/JPS63501767A/ja active Pending
- 1986-11-13 DE DE8686907170T patent/DE3687796T2/de not_active Expired - Fee Related
- 1986-11-13 EP EP86907170A patent/EP0245479B1/de not_active Expired - Lifetime
- 1986-11-13 AU AU66222/86A patent/AU621277B2/en not_active Ceased
- 1986-11-13 WO PCT/US1986/002443 patent/WO1987003008A1/en not_active Ceased
- 1986-11-17 CA CA000523097A patent/CA1312838C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP0245479A1 (de) | 1987-11-19 |
| AU6622286A (en) | 1987-06-02 |
| EP0245479B1 (de) | 1993-02-17 |
| JPS63501767A (ja) | 1988-07-21 |
| CA1312838C (en) | 1993-01-19 |
| EP0245479A4 (de) | 1989-04-12 |
| AU621277B2 (en) | 1992-03-12 |
| DE3687796D1 (en) | 1993-03-25 |
| WO1987003008A1 (en) | 1987-05-21 |
| DE3687796T2 (de) | 1993-06-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| IE821985L (en) | Expression vectors | |
| AU590733B2 (en) | Aspergillus niger transformation system | |
| DK75694A (da) | Tumornecrosefaktor og derivater og mutantformer deraf, fremgangsmåde til fremstilling deraf, DNA som koder derfor, replicerbar udtrykkelsesvektor indeholdende DNA'en og celle transformeret dermed samt præparater omfattende tumornecrosefaktoren | |
| UA42715C2 (uk) | Полінуклеотидна вакцина на основі плазмідної днк, імуногенна композиція(варіанти) | |
| YU46695B (sh) | Regulatorni region za ekspresiju heterologog gena u kvascu | |
| IL90423A0 (en) | Interleukin-1 inhibitors | |
| ATE262588T1 (de) | Plasmid für il-2 expression | |
| AU2812484A (en) | Dna vectors and there use in recombinant dna technology | |
| DE69214146D1 (de) | Deletionsmutanten von bovinem herpuvirus-1, auf diese basierte impfstoffe,diagnosesätze für den nachweis von bovinem herpesvirus-1 | |
| Li et al. | Detection of direct binding of human herpesvirus 8-encoded interleukin-6 (vIL-6) to both gp130 and IL-6 receptor (IL-6R) and identification of amino acid residues of vIL-6 important for IL-6R-dependent and-independent signaling | |
| EP0192033A3 (de) | Hauptprotein der Aussenmembran von Chlamydia | |
| EP0077649A3 (de) | Klonierungsvektoren zur Verwendung in Streptomycinen | |
| ATE85812T1 (de) | Verkuerzter phosphoglycerat-kinase-promotor. | |
| NO880470L (no) | Nytt ekspresjonssystem. | |
| FR2679253B1 (fr) | Proteines de resistance a la cycloheximide. utilisation comme marqueur de selection par exemple pour controler le transfert d'acides nucleiques. | |
| AU582288B2 (en) | Vector and method for achieving high level expression in eukaryotic cells | |
| EP0811685A3 (de) | Menschliche Rezeptoren für den von Blutplättchen abstammenden Wachstumsfaktor | |
| ATE53606T1 (de) | Mit einem herpes-simplex-virus-gen inseriertes rekombinantes plasmid. | |
| ATE48156T1 (de) | Verfahren zur heterologen klonierung eines gens in einem bacillus-mikroorganismus. | |
| DK261183D0 (da) | Temperaturfoelsomme multikopiplasmider | |
| EP0232845A3 (de) | Induzierbare Wärmeschock- und Amplifizierungssysteme | |
| EP0068647A3 (de) | Plasmide, Plasmidwirte und deren Herstellung | |
| Garman et al. | Chromatographic separation from known cytokines of a helper factor necessary for the generation of murine cytolytic T lymphocytes. | |
| ATE45185T1 (de) | Plasmid phjl210 und verwandte bifunktionelle klonierungsvektoren zur verwendung in streptomyces. | |
| Frazier et al. | Roles of the Escherichia coli heat shock sigma factor 32 in early and late gene expression of bacteriophage T4 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |