ATE57714T1 - Verfahren zur herstellung eines stammes, insbesondere von hefe, transformiert mit einem expressionsvektor, der gezuechtet werden kann in einem kompletten medium ohne selektionsdruck und auf diese weise erhaltener stamm. - Google Patents

Verfahren zur herstellung eines stammes, insbesondere von hefe, transformiert mit einem expressionsvektor, der gezuechtet werden kann in einem kompletten medium ohne selektionsdruck und auf diese weise erhaltener stamm.

Info

Publication number
ATE57714T1
ATE57714T1 AT85401620T AT85401620T ATE57714T1 AT E57714 T1 ATE57714 T1 AT E57714T1 AT 85401620 T AT85401620 T AT 85401620T AT 85401620 T AT85401620 T AT 85401620T AT E57714 T1 ATE57714 T1 AT E57714T1
Authority
AT
Austria
Prior art keywords
strain
transformed
expression vector
complete medium
selection pressure
Prior art date
Application number
AT85401620T
Other languages
English (en)
Inventor
Gerard Loison
Original Assignee
Transgene Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transgene Sa filed Critical Transgene Sa
Application granted granted Critical
Publication of ATE57714T1 publication Critical patent/ATE57714T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8121—Serpins
    • C07K14/8125—Alpha-1-antitrypsin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
AT85401620T 1984-08-09 1985-08-08 Verfahren zur herstellung eines stammes, insbesondere von hefe, transformiert mit einem expressionsvektor, der gezuechtet werden kann in einem kompletten medium ohne selektionsdruck und auf diese weise erhaltener stamm. ATE57714T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR8412598A FR2568891B1 (fr) 1984-08-09 1984-08-09 Procede de preparation d'une souche, notamment de levure, transformee par un vecteur d'expression, qui peut etre cultivee sur un milieu complet sans pression de selection et souche ainsi obtenue
EP85401620A EP0173619B1 (de) 1984-08-09 1985-08-08 Verfahren zur Herstellung eines Stammes, insbesondere von Hefe, transformiert mit einem Expressionsvektor, der gezüchtet werden kann in einem kompletten Medium ohne Selektionsdruck und auf diese Weise erhaltener Stamm

Publications (1)

Publication Number Publication Date
ATE57714T1 true ATE57714T1 (de) 1990-11-15

Family

ID=9306937

Family Applications (1)

Application Number Title Priority Date Filing Date
AT85401620T ATE57714T1 (de) 1984-08-09 1985-08-08 Verfahren zur herstellung eines stammes, insbesondere von hefe, transformiert mit einem expressionsvektor, der gezuechtet werden kann in einem kompletten medium ohne selektionsdruck und auf diese weise erhaltener stamm.

Country Status (8)

Country Link
EP (1) EP0173619B1 (de)
JP (1) JPS61502939A (de)
AT (1) ATE57714T1 (de)
CA (1) CA1268722A (de)
DE (1) DE3580222D1 (de)
DK (1) DK157486D0 (de)
FR (1) FR2568891B1 (de)
WO (1) WO1986001224A1 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8530631D0 (en) 1985-12-12 1986-01-22 Ciba Geigy Ag Thrombin inhibitors
MY101203A (en) * 1985-12-12 1991-08-17 Ucp Gen Pharma Ag Production of thrombin iinhibitors.
US5922583A (en) * 1995-10-17 1999-07-13 Biostar Inc. Methods for production of recombinant plasmids
GB2318732A (en) 1996-11-01 1998-05-06 Johnson & Johnson Medical Wound healing compositions containing alpha-1-antitrypsin

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2441659A1 (fr) * 1978-11-14 1980-06-13 Anvar Nouveaux plasmides hybrides et microorganismes les contenant
FR2489364A1 (fr) * 1980-08-29 1982-03-05 Centre Nat Rech Scient Nouveaux plasmides hybrides et microorganismes les comportant

Also Published As

Publication number Publication date
WO1986001224A1 (fr) 1986-02-27
FR2568891B1 (fr) 1988-05-27
DK157486A (da) 1986-04-07
CA1268722A (fr) 1990-05-08
DK157486D0 (da) 1986-04-07
JPS61502939A (ja) 1986-12-18
EP0173619A1 (de) 1986-03-05
DE3580222D1 (de) 1990-11-29
EP0173619B1 (de) 1990-10-24
FR2568891A1 (fr) 1986-02-14

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UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee