ATE544869T1 - Verfahren und substanzen zur isolierung und erkennung kleiner polynukleotide - Google Patents
Verfahren und substanzen zur isolierung und erkennung kleiner polynukleotideInfo
- Publication number
- ATE544869T1 ATE544869T1 AT07841673T AT07841673T ATE544869T1 AT E544869 T1 ATE544869 T1 AT E544869T1 AT 07841673 T AT07841673 T AT 07841673T AT 07841673 T AT07841673 T AT 07841673T AT E544869 T1 ATE544869 T1 AT E544869T1
- Authority
- AT
- Austria
- Prior art keywords
- small
- isolation
- substances
- detection
- small polynucleotide
- Prior art date
Links
- 102000040430 polynucleotide Human genes 0.000 title abstract 6
- 108091033319 polynucleotide Proteins 0.000 title abstract 6
- 239000002157 polynucleotide Substances 0.000 title abstract 6
- 238000000034 method Methods 0.000 title abstract 4
- 238000002955 isolation Methods 0.000 title abstract 2
- 238000001514 detection method Methods 0.000 title 1
- 239000000126 substance Substances 0.000 title 1
- 239000000523 sample Substances 0.000 abstract 4
- 230000003321 amplification Effects 0.000 abstract 1
- 238000003776 cleavage reaction Methods 0.000 abstract 1
- 230000009977 dual effect Effects 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 238000002372 labelling Methods 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 230000007017 scission Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US82406806P | 2006-08-30 | 2006-08-30 | |
| US82588806P | 2006-09-15 | 2006-09-15 | |
| US86388606P | 2006-11-01 | 2006-11-01 | |
| US86621006P | 2006-11-16 | 2006-11-16 | |
| US87109406P | 2006-12-20 | 2006-12-20 | |
| PCT/US2007/077311 WO2008028086A2 (en) | 2006-08-30 | 2007-08-30 | Methods and substances for isolation and detection of small polynucleotides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE544869T1 true ATE544869T1 (de) | 2012-02-15 |
Family
ID=39136920
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT07841673T ATE544869T1 (de) | 2006-08-30 | 2007-08-30 | Verfahren und substanzen zur isolierung und erkennung kleiner polynukleotide |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US8329394B2 (de) |
| EP (1) | EP2057181B1 (de) |
| CN (1) | CN101528763B (de) |
| AT (1) | ATE544869T1 (de) |
| CA (1) | CA2662837C (de) |
| WO (1) | WO2008028086A2 (de) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2445204C (en) | 2002-10-16 | 2014-08-12 | Streck Laboratories, Inc. | Method and device for collecting and preserving cells for analysis |
| DE602006011917D1 (de) * | 2005-08-19 | 2010-03-11 | Bioventures Inc | Verfahren und substanzen zur isolierung von mirnas |
| US20110172105A1 (en) * | 2008-06-04 | 2011-07-14 | Salk Institute For Biological Studies | GREPSEQ: An Almost Inexhaustible, Cost-Effective, High-Throughput Protocol for the Generation of Selector Sequences |
| US11634747B2 (en) | 2009-01-21 | 2023-04-25 | Streck Llc | Preservation of fetal nucleic acids in maternal plasma |
| DK2398912T3 (en) | 2009-02-18 | 2017-10-30 | Streck Inc | Conservation of cell-free nucleic acids |
| US20130296189A1 (en) * | 2009-05-08 | 2013-11-07 | Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences | Probes utilizing universal tags, a kit comprising the same and detection methods |
| ES2571104T3 (es) * | 2009-11-09 | 2016-05-24 | Streck Inc | Estabilización del ARN y extracción del ARN presente en células intactas dentro de una muestra de sangre |
| US9956281B2 (en) | 2011-05-04 | 2018-05-01 | Streck, Inc. | Inactivated virus compositions and methods of preparing such compositions |
| AU2012284202B2 (en) * | 2011-07-15 | 2015-12-17 | Orasure Technologies, Inc. | Sample collection kit |
| GB2493141A (en) * | 2011-07-19 | 2013-01-30 | Gene Onyx Ltd | Method of selecting a product using a DNA sample |
| ES2938048T3 (es) | 2013-07-24 | 2023-04-04 | Streck Llc | Composiciones y procedimientos para estabilizar las células tumorales circulantes |
| CN103484544B (zh) * | 2013-09-17 | 2015-11-25 | 遵义医学院 | 一种微小rna启动子活性检测的方法 |
| CN105274194A (zh) * | 2014-12-10 | 2016-01-27 | 临沂大学 | 一种基于多重放大技术的肿瘤标志物let7a检测方法 |
| US11168351B2 (en) | 2015-03-05 | 2021-11-09 | Streck, Inc. | Stabilization of nucleic acids in urine |
| US20170145475A1 (en) | 2015-11-20 | 2017-05-25 | Streck, Inc. | Single spin process for blood plasma separation and plasma composition including preservative |
| WO2017096322A1 (en) * | 2015-12-03 | 2017-06-08 | Accuragen Holdings Limited | Methods and compositions for forming ligation products |
| US11098360B2 (en) | 2016-06-01 | 2021-08-24 | Roche Sequencing Solutions, Inc. | Immuno-PETE |
| WO2018022991A1 (en) | 2016-07-29 | 2018-02-01 | Streck, Inc. | Suspension composition for hematology analysis control |
| GB201701262D0 (en) * | 2017-01-25 | 2017-03-08 | Sense Biodetection Ltd | Nucleic acid detection method |
| US12378543B2 (en) | 2017-10-19 | 2025-08-05 | Streck Llc | Compositions for hemolysis and coagulation regulation and stabilization of extracellular vesicles |
| JP2021506314A (ja) * | 2017-12-21 | 2021-02-22 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 単一方向デュアルプローブプライマー伸長による標的濃縮 |
| WO2020054700A1 (ja) * | 2018-09-11 | 2020-03-19 | 積水メディカル株式会社 | オリゴヌクレオチドの検出、または定量方法 |
| CN110358810B (zh) * | 2019-07-29 | 2023-06-23 | 江苏省原子医学研究所 | 一种检测miRNA的生物探针及检测方法和用途 |
| CN113088560B (zh) * | 2021-04-16 | 2022-02-25 | 杭州德同生物技术有限公司 | 基于杂交捕获及酶保护的microRNA高通量检测方法及试剂盒 |
| AU2022426779A1 (en) * | 2021-12-29 | 2024-07-18 | Co-Diagnostics, Inc. | Methods and systems for analyzing nucleic acids using increased ifret with multiple acceptor fluorophores |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5629153A (en) * | 1990-01-10 | 1997-05-13 | Chiron Corporation | Use of DNA-dependent RNA polymerase transcripts as reporter molecules for signal amplification in nucleic acid hybridization assays |
| WO1994016105A1 (en) * | 1993-01-15 | 1994-07-21 | The Public Health Research Institute Of The City Of New York, Inc. | Rna assays using rna binary probes and ribozyme ligase |
| US6045802A (en) * | 1994-10-03 | 2000-04-04 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Enhanced immune response to an antigen by a composition of a recombinant virus expressing the antigen with a recombinant virus expressing an immunostimulatory molecule |
| US5750341A (en) * | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
| WO1998006437A2 (en) * | 1996-08-13 | 1998-02-19 | Chiron Corporation | Compositions and methods for polynucleotide delivery |
| US7101672B2 (en) * | 1998-05-05 | 2006-09-05 | Third Wave Technologies, Inc. | Target-dependent reactions using structure-bridging oligonucleotides |
| US6365731B1 (en) * | 1997-08-06 | 2002-04-02 | Ambion, Inc. | Stripping nucleic acids with iodine and sodium thiosulfate |
| AU756301B2 (en) * | 1997-08-20 | 2003-01-09 | Somagenics, Inc. | Antisense and antigene therapeutics with improved binding properties and methods for their use |
| WO1999028505A1 (en) | 1997-12-03 | 1999-06-10 | Curagen Corporation | Methods and devices for measuring differential gene expression |
| US6391551B1 (en) * | 1998-03-13 | 2002-05-21 | Promega Corporation | Detection of nucleic acid hybrids |
| SG2011071982A (en) * | 2001-05-25 | 2016-09-29 | Univ Duke | Modulators of pharmacological agents |
| US8192937B2 (en) | 2004-04-07 | 2012-06-05 | Exiqon A/S | Methods for quantification of microRNAs and small interfering RNAs |
| US20050277139A1 (en) * | 2004-04-26 | 2005-12-15 | Itzhak Bentwich | Methods and apparatus for the detection and validation of microRNAs |
| DE602006011917D1 (de) * | 2005-08-19 | 2010-03-11 | Bioventures Inc | Verfahren und substanzen zur isolierung von mirnas |
| US20070077582A1 (en) * | 2005-09-16 | 2007-04-05 | Primera Biosystems, Inc. | Method for quantitative detection of short RNA molecules |
-
2007
- 2007-08-30 EP EP07841673A patent/EP2057181B1/de active Active
- 2007-08-30 WO PCT/US2007/077311 patent/WO2008028086A2/en not_active Ceased
- 2007-08-30 AT AT07841673T patent/ATE544869T1/de active
- 2007-08-30 CA CA2662837A patent/CA2662837C/en active Active
- 2007-08-30 CN CN2007800319763A patent/CN101528763B/zh not_active Expired - Fee Related
- 2007-12-17 US US11/958,180 patent/US8329394B2/en active Active
-
2012
- 2012-11-15 US US13/678,368 patent/US20130116154A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP2057181B1 (de) | 2012-02-08 |
| WO2008028086A2 (en) | 2008-03-06 |
| US20080102470A1 (en) | 2008-05-01 |
| US8329394B2 (en) | 2012-12-11 |
| WO2008028086A3 (en) | 2008-11-13 |
| CN101528763B (zh) | 2012-06-27 |
| EP2057181A2 (de) | 2009-05-13 |
| WO2008028086A9 (en) | 2009-04-02 |
| US20130116154A1 (en) | 2013-05-09 |
| EP2057181A4 (de) | 2009-12-02 |
| CN101528763A (zh) | 2009-09-09 |
| CA2662837C (en) | 2012-07-31 |
| CA2662837A1 (en) | 2008-03-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ATE544869T1 (de) | Verfahren und substanzen zur isolierung und erkennung kleiner polynukleotide | |
| WO2009022125A8 (en) | Identification of nucleic acid sequences | |
| EP4592400A3 (de) | Verfahren und produkt zur optimierung einer lokalisierten oder räumlichen detektion einer genexpression in einer gewebeprobe | |
| MX2013011737A (es) | Metodo y producto para le deteccion localizada o espacial de acido nucleico en una muestra de tejido. | |
| WO2012149188A3 (en) | Compositions and methods for detecting and identifying nucleic acid sequences in biological samples | |
| WO2011022093A3 (en) | Methods and devices for detecting the presence of an analyte in a sample | |
| WO2008014485A3 (en) | Multiplex q-pcr arrays | |
| WO2011063388A3 (en) | Assay method for target nucleic acid by signal amplification using probe hybridization and restriction | |
| WO2007083147A3 (en) | High throughput testing for presence of microorganisms in a biological sample | |
| WO2007044974A3 (en) | Absolute pcr quantification | |
| WO2011146629A3 (en) | Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids | |
| HK1250245A1 (zh) | 生物样本的空间区别、多重核酸分析 | |
| WO2011143478A3 (en) | Identification of nucleic acids | |
| GB201008439D0 (en) | Method for detecting nucleic acids by simultaneous isothermal amplification of nucleic acids and signal probe | |
| CL2011002228A1 (es) | Aparato para el analisis integrado de acidos nucleicos en tiempo real, y metodo de detecicion de dichas moleculas. | |
| WO2011153354A8 (en) | Methods and kits for in situ detection of nucleotide sequences | |
| WO2013009084A3 (ko) | 평행결합 구조의 이중 혼성화 pna 프로브 시스템을 포함하는 실시간 다중 중합효소 연쇄반응에 의한 결핵균 및 비결핵 항산균의 동시 검출용 조성물 및 이를 이용한 검출 방법 | |
| WO2011087789A3 (en) | Methods for the detection of microorganisms | |
| WO2008054830A3 (en) | Dna array analysis as a diagnostic for current and emerging strains of influenza | |
| WO2013090386A3 (en) | Methods and kits for room temperature in situ detection of a target nucleic acid in a biological sample | |
| WO2010096202A3 (en) | Sequence-specific detection of nucleotide sequences | |
| HK1243421A1 (zh) | 检测核酸的组合物和方法 | |
| WO2007120808A3 (en) | A method of asymmetric helicase-dependent amplification for probe-based detection of targets | |
| ATE546550T1 (de) | Verfahren zum nachweis von hpv sowie sonden, primer und kits | |
| WO2009000764A9 (de) | Verfahren und schnelltest zum nachweis spezifischer nukleinsäuresequenzen |