ATE520788T1 - SÄUGETIER-ßTWO-HYBRIDß-SYSTEM ZUM SCREENEN VON AKKUMULATIONS-MODULATOREN VON METABOLISCHEN PRODUKTEN - Google Patents
SÄUGETIER-ßTWO-HYBRIDß-SYSTEM ZUM SCREENEN VON AKKUMULATIONS-MODULATOREN VON METABOLISCHEN PRODUKTENInfo
- Publication number
- ATE520788T1 ATE520788T1 AT01977707T AT01977707T ATE520788T1 AT E520788 T1 ATE520788 T1 AT E520788T1 AT 01977707 T AT01977707 T AT 01977707T AT 01977707 T AT01977707 T AT 01977707T AT E520788 T1 ATE520788 T1 AT E520788T1
- Authority
- AT
- Austria
- Prior art keywords
- metabolic product
- cell
- chimeric protein
- ligand
- protein comprises
- Prior art date
Links
- 230000002503 metabolic effect Effects 0.000 title abstract 10
- 238000009825 accumulation Methods 0.000 title 1
- 238000012216 screening Methods 0.000 title 1
- 102000037865 fusion proteins Human genes 0.000 abstract 6
- 108020001507 fusion proteins Proteins 0.000 abstract 6
- 210000004027 cell Anatomy 0.000 abstract 5
- 108090000623 proteins and genes Proteins 0.000 abstract 5
- 239000003795 chemical substances by application Substances 0.000 abstract 4
- 239000003446 ligand Substances 0.000 abstract 4
- 239000012636 effector Substances 0.000 abstract 3
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 2
- 108091007494 Nucleic acid- binding domains Proteins 0.000 abstract 1
- 230000004075 alteration Effects 0.000 abstract 1
- 239000002299 complementary DNA Substances 0.000 abstract 1
- 230000007547 defect Effects 0.000 abstract 1
- 210000004962 mammalian cell Anatomy 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 238000010396 two-hybrid screening Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
- A61K48/005—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
- A61K48/0058—Nucleic acids adapted for tissue specific expression, e.g. having tissue specific promoters as part of a contruct
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Epidemiology (AREA)
- Crystallography & Structural Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/687,593 US6787321B1 (en) | 2000-10-13 | 2000-10-13 | Mammalian two-hybrid system for screening for modulators of the accumulation of metabolic products |
| PCT/US2001/031745 WO2002031201A2 (en) | 2000-10-13 | 2001-10-09 | Mammalian two-hybrid system for screening for modulators of the accumulation of metabolic products |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE520788T1 true ATE520788T1 (de) | 2011-09-15 |
Family
ID=24761035
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT01977707T ATE520788T1 (de) | 2000-10-13 | 2001-10-09 | SÄUGETIER-ßTWO-HYBRIDß-SYSTEM ZUM SCREENEN VON AKKUMULATIONS-MODULATOREN VON METABOLISCHEN PRODUKTEN |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US6787321B1 (https=) |
| EP (1) | EP1327003B1 (https=) |
| JP (1) | JP2004511237A (https=) |
| AT (1) | ATE520788T1 (https=) |
| AU (1) | AU2001296805A1 (https=) |
| CA (1) | CA2424317A1 (https=) |
| WO (1) | WO2002031201A2 (https=) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE524559T1 (de) * | 2002-12-18 | 2011-09-15 | Chromagenics Bv | Methode zur verbesserung der proteinproduktion |
| WO2006125031A2 (en) * | 2005-05-19 | 2006-11-23 | Wyeth | Method of identifying compounds that modulate interaction of androgen receptor with beta-catenin |
| US20100099096A1 (en) * | 2007-02-28 | 2010-04-22 | The Brigham And Women's Hospital, Inc. | Compositions and Methods for Identifying Factors Affecting Protein Stability |
| EP2176409B1 (en) | 2007-07-11 | 2012-01-04 | Yeda Research And Development Co. Ltd. | Nucleic acid construct systems capable of diagnosing or treating a cell state |
| US20120156180A1 (en) * | 2009-08-10 | 2012-06-21 | The Board Of Trustees Of The University Of Illinois | Compositions and methods for the treatment of krabbe and other neurodegenerative diseases |
| GB2584211B (en) * | 2017-11-16 | 2023-05-24 | Synthex Inc | Selective modulation of protein-protein interactions |
| EP3969592A4 (en) * | 2019-05-15 | 2023-01-04 | Synthex, Inc. | SELECTIVE BREAKDOWN OF PROTEINS |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0370813A3 (en) | 1988-11-25 | 1991-06-19 | Exemplar Corporation | Rapid screening mutagenesis and teratogenesis assay |
| US5283173A (en) | 1990-01-24 | 1994-02-01 | The Research Foundation Of State University Of New York | System to detect protein-protein interactions |
| US5525490A (en) | 1994-03-29 | 1996-06-11 | Onyx Pharmaceuticals, Inc. | Reverse two-hybrid method |
| NZ306767A (en) | 1995-04-11 | 2000-03-27 | Univ Johns Hopkins | Method of identifying molecular interactions employing counterselection and at least two hybrid molecules or two hybrid systems |
| FR2734567B1 (fr) | 1995-05-26 | 1997-08-08 | Commissariat Energie Atomique | Proteines chimeriques activant la transcription par la polymerase iii, leur utilisation pour la detection et l'analyse des interactions entre proteines, et genes codant pour lesdites proteines |
| USH1892H (en) | 1995-07-27 | 2000-10-03 | Pharmacia And Upjohn Company | High volume nutrient based yeast two-hybrid assay for the identification of specific protein:protein interacting inhibitors |
| US5948620A (en) | 1996-08-05 | 1999-09-07 | Amersham International Plc | Reverse two-hybrid system employing post-translation signal modulation |
| US6133027A (en) | 1996-08-07 | 2000-10-17 | City Of Hope | Inducible expression system |
| US6051381A (en) | 1996-12-11 | 2000-04-18 | Kornacker; Michael G. | Prokaryotic two-hybrid system |
| US5851775A (en) * | 1997-03-20 | 1998-12-22 | Johns Hopkins University | β-catenin, Tcf-4, and APC interact to prevent cancer |
| US5885779A (en) * | 1997-09-09 | 1999-03-23 | University Of British Columbia | Repressed trans-activator system for characterization of protein-protein interactions |
| US6242253B1 (en) * | 1997-10-09 | 2001-06-05 | Regents Of The University Of California | IkB kinase, subunits thereof, and methods of using same |
-
2000
- 2000-10-13 US US09/687,593 patent/US6787321B1/en not_active Expired - Lifetime
-
2001
- 2001-10-09 AT AT01977707T patent/ATE520788T1/de not_active IP Right Cessation
- 2001-10-09 CA CA002424317A patent/CA2424317A1/en not_active Abandoned
- 2001-10-09 WO PCT/US2001/031745 patent/WO2002031201A2/en not_active Ceased
- 2001-10-09 EP EP01977707A patent/EP1327003B1/en not_active Expired - Lifetime
- 2001-10-09 AU AU2001296805A patent/AU2001296805A1/en not_active Abandoned
- 2001-10-09 JP JP2002534565A patent/JP2004511237A/ja active Pending
-
2004
- 2004-07-21 US US10/896,600 patent/US7358086B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP1327003B1 (en) | 2011-08-17 |
| AU2001296805A1 (en) | 2002-04-22 |
| CA2424317A1 (en) | 2002-04-18 |
| WO2002031201A3 (en) | 2002-11-21 |
| US20040265791A1 (en) | 2004-12-30 |
| US6787321B1 (en) | 2004-09-07 |
| EP1327003A2 (en) | 2003-07-16 |
| JP2004511237A (ja) | 2004-04-15 |
| US7358086B2 (en) | 2008-04-15 |
| WO2002031201A2 (en) | 2002-04-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Tugyi et al. | Partial D-amino acid substitution: Improved enzymatic stability and preserved Ab recognition of a MUC2 epitope peptide | |
| MXPA04002808A (es) | Acidos nucleicos receptores de ecdisona de mosca blanca, polipeptidos, y sus usos. | |
| ATE301188T1 (de) | Reprimiertes trans-aktivatorsystem zur charakterisierung von protein-protein interaktionen | |
| WO2005010215A3 (en) | Methods for identifying tolerance modulatory compounds and uses therefor | |
| WO2002057303A3 (en) | Protein-protein interactions between shigella flexneri polypeptides and mammalian polypeptides | |
| EP0921395A3 (en) | Assays for measurement of protein fragments in biological media | |
| ATE346160T1 (de) | Proteingerüste für antikörper-mimetika und andere bindungsproteine | |
| DE69929466D1 (de) | Polypeptidzusammensetzungen von bacillus thuringiensis cryet70 die für diabrotica insekten toxisch sind, und methoden zur dessen verwendung | |
| PT692025E (pt) | Um metodo de determinacao do actual montante de dinheiro num portador de dados e sistema para implementar este metodo | |
| DE69834334D1 (de) | Für adipozyten spezifische protein homologe | |
| DE69826888D1 (de) | Bakterielles multi-hybrid system und anwendungen dafür | |
| ATE520788T1 (de) | SÄUGETIER-ßTWO-HYBRIDß-SYSTEM ZUM SCREENEN VON AKKUMULATIONS-MODULATOREN VON METABOLISCHEN PRODUKTEN | |
| DE69834643D1 (de) | Adipozyten-spezifische protein homologe | |
| AU2002239229A1 (en) | Receptor for b. anthracis toxin | |
| CA2117612A1 (en) | Periplasmic membrane-bound system for detecting protein-protein interactions | |
| Onions et al. | A Novel Type of Calmodulin Interaction in the Inhibition of Basic Helix− Loop− Helix Transcription Factors | |
| WO2001072956A3 (en) | Compounds and methods for modulating desmosomal cadherin-mediated functions | |
| Kokai-Kun et al. | Determination of functional regions of Clostridium perfringens enterotoxin through deletion analysis | |
| WO2002061046A3 (en) | Novel nucleic acid and polypeptide molecules | |
| Qin et al. | Alternative mode of binding to phosphotyrosyl peptides by Src homology-2 domains | |
| DE60104019D1 (de) | Zusammensetzungen, Verfahren und Reagentiensätze zur Erkennung von Protein-Protein Wechselwirkung störenden Substanzen | |
| NO20052495L (no) | Induserbar ligand for alfa1 beta1 integrin og anvendelse derav. | |
| WO2003052078A3 (en) | Insect g protein-coupled receptor genes and uses thereof | |
| DE59914992D1 (de) | Methode zur zellulären high-throughput-detektion von nukleären rezeptor-liganden-interaktionen | |
| WO2002050104A3 (en) | Methods and cells for detecting modulators of rgs proteins |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |