CA2424317A1 - Mammalian two-hybrid system for screening for modulators of the accumulation of metabolic products - Google Patents
Mammalian two-hybrid system for screening for modulators of the accumulation of metabolic products Download PDFInfo
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- CA2424317A1 CA2424317A1 CA002424317A CA2424317A CA2424317A1 CA 2424317 A1 CA2424317 A1 CA 2424317A1 CA 002424317 A CA002424317 A CA 002424317A CA 2424317 A CA2424317 A CA 2424317A CA 2424317 A1 CA2424317 A1 CA 2424317A1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
- A61K48/005—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
- A61K48/0058—Nucleic acids adapted for tissue specific expression, e.g. having tissue specific promoters as part of a contruct
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
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- Animal Behavior & Ethology (AREA)
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- Crystallography & Structural Chemistry (AREA)
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- Analytical Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Immunology (AREA)
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- Chemical Kinetics & Catalysis (AREA)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
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| US09/687,593 US6787321B1 (en) | 2000-10-13 | 2000-10-13 | Mammalian two-hybrid system for screening for modulators of the accumulation of metabolic products |
| US09/687,593 | 2000-10-13 | ||
| PCT/US2001/031745 WO2002031201A2 (en) | 2000-10-13 | 2001-10-09 | Mammalian two-hybrid system for screening for modulators of the accumulation of metabolic products |
Publications (1)
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| CA2424317A1 true CA2424317A1 (en) | 2002-04-18 |
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|---|---|
| US (2) | US6787321B1 (https=) |
| EP (1) | EP1327003B1 (https=) |
| JP (1) | JP2004511237A (https=) |
| AT (1) | ATE520788T1 (https=) |
| AU (1) | AU2001296805A1 (https=) |
| CA (1) | CA2424317A1 (https=) |
| WO (1) | WO2002031201A2 (https=) |
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| EP1573058B1 (en) * | 2002-12-18 | 2011-09-14 | ChromaGenics B.V. | A method for improving protein production |
| EP1896583A2 (en) * | 2005-05-19 | 2008-03-12 | Wyeth | Method of identifying compounds that modulate interaction of androgen receptor with beta-catenin |
| US20100099096A1 (en) * | 2007-02-28 | 2010-04-22 | The Brigham And Women's Hospital, Inc. | Compositions and Methods for Identifying Factors Affecting Protein Stability |
| US20100197767A1 (en) | 2007-07-11 | 2010-08-05 | Lior Nissim | Nucleic acid construct systems capable of daignosing or treating a cell state |
| WO2011019763A2 (en) * | 2009-08-10 | 2011-02-17 | The Board Of Trustees Of The University Of Illinois | Compositions and methods for the treatment of krabbe and other neurodegenerative diseases |
| GB2584211B (en) * | 2017-11-16 | 2023-05-24 | Synthex Inc | Selective modulation of protein-protein interactions |
| JP7773376B2 (ja) | 2019-05-15 | 2025-11-19 | シンセックス， インコーポレイテッド | タンパク質の選択的分解 |
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| EP0370813A3 (en) | 1988-11-25 | 1991-06-19 | Exemplar Corporation | Rapid screening mutagenesis and teratogenesis assay |
| US5283173A (en) | 1990-01-24 | 1994-02-01 | The Research Foundation Of State University Of New York | System to detect protein-protein interactions |
| US5525490A (en) | 1994-03-29 | 1996-06-11 | Onyx Pharmaceuticals, Inc. | Reverse two-hybrid method |
| DE69636866D1 (en) | 1995-04-11 | 2007-03-15 | Gen Hospital Corp | Reverse "two-hybrid"-systeme |
| FR2734567B1 (fr) | 1995-05-26 | 1997-08-08 | Commissariat Energie Atomique | Proteines chimeriques activant la transcription par la polymerase iii, leur utilisation pour la detection et l'analyse des interactions entre proteines, et genes codant pour lesdites proteines |
| USH1892H (en) | 1995-07-27 | 2000-10-03 | Pharmacia And Upjohn Company | High volume nutrient based yeast two-hybrid assay for the identification of specific protein:protein interacting inhibitors |
| US5948620A (en) | 1996-08-05 | 1999-09-07 | Amersham International Plc | Reverse two-hybrid system employing post-translation signal modulation |
| US6133027A (en) | 1996-08-07 | 2000-10-17 | City Of Hope | Inducible expression system |
| DE69732537T2 (de) | 1996-12-11 | 2006-03-30 | Bristol-Myers Squibb Co. | Prokaryotisches zwei-hybrid system |
| US5851775A (en) * | 1997-03-20 | 1998-12-22 | Johns Hopkins University | β-catenin, Tcf-4, and APC interact to prevent cancer |
| US5885779A (en) * | 1997-09-09 | 1999-03-23 | University Of British Columbia | Repressed trans-activator system for characterization of protein-protein interactions |
| US6242253B1 (en) * | 1997-10-09 | 2001-06-05 | Regents Of The University Of California | IkB kinase, subunits thereof, and methods of using same |
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2004
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Also Published As
| Publication number | Publication date |
|---|---|
| AU2001296805A1 (en) | 2002-04-22 |
| JP2004511237A (ja) | 2004-04-15 |
| US7358086B2 (en) | 2008-04-15 |
| ATE520788T1 (de) | 2011-09-15 |
| US6787321B1 (en) | 2004-09-07 |
| WO2002031201A3 (en) | 2002-11-21 |
| US20040265791A1 (en) | 2004-12-30 |
| WO2002031201A2 (en) | 2002-04-18 |
| EP1327003B1 (en) | 2011-08-17 |
| EP1327003A2 (en) | 2003-07-16 |
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